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37 results on '"van den Broek, Tim J."'

14. Impact of Microbiota and Metabolites on Intestinal Integrity and Inflammation in Severe Obesity.

Author

Custers, Emma, Vreeken, Debby, Schuren, Frank, van den Broek, Tim J., van Dongen, Lieke, Geenen, Bram, de Blaauw, Ivo, Wiesmann, Maximilian, Hazebroek, Eric J., Kleemann, Robert, and Kiliaan, Amanda J.

Subjects
CYTOTOXIC T cells, GUT microbiome, TIGHT junctions, CELL junctions, MAST cells
Abstract

Obesity is a multifactorial disease associated with low-grade inflammation. The gut is thought to be involved in obesity-related inflammation, as it is continuously exposed to antigens from food, microbiota and metabolites. However, the exact underlying mechanisms are still unknown. Therefore, we examined the relation between gut pathology, microbiota, its metabolites and cytokines in adults with severe obesity. Individuals eligible for bariatric surgery were included. Fecal and plasma samples were collected at surgery timepoint, to assess microbiota and metabolite composition. Jejunal biopsies were collected during surgery and stained for cytotoxic T cells, macrophages, mast cells and tight junction component zonula occludens-1. Based on these stainings, the cohort was divided into four groups: high versus low intestinal inflammation and high versus low intestinal integrity. We found no significant differences in microbiota diversity between groups, nor for individual bacterial species. No significant differences in metabolites were observed between the intestinal inflammatory groups. However, some metabolites and cytokines differed between the intestinal integrity groups. Higher plasma levels of interleukin-8 and tauro-chenodeoxycholic acid were found, whereas isovaleric acid and acetic acid were lower in the high intestinal integrity group. As the results were very subtle, we suggest that our cohort shows very early and minor intestinal pathology. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Uncovering a Predictive Molecular Signature for the Onset of NASH-Related Fibrosis in a Translational NASH Mouse Model

Author

van Koppen, Arianne, Verschuren, Lars, van den Hoek, Anita M., Verheij, Joanne, Morrison, Martine C., Li, Kelvin, Nagabukuro, Hiroshi, Costessi, Adalberto, Caspers, Martien P.M., van den Broek, Tim J., Sagartz, John, Kluft, Cornelis, Beysen, Carine, Emson, Claire, van Gool, Alain J., Goldschmeding, Roel, Stoop, Reinout, Bobeldijk-Pastorova, Ivana, Turner, Scott M., Hanauer, Guido, and Hanemaaijer, Roeland

Published
2018
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19. 2’-Fucosyllactose inhibits proliferation of Clostridioides difficile ATCC 43599 in the CDi-screen, an in vitro model simulating Clostridioides difficile infection

Author

Wiese, Maria, primary, Schuren, Frank H. J., additional, Smits, Wiep Klaas, additional, Kuijper, Ed J., additional, Ouwens, Anita, additional, Heerikhuisen, Margreet, additional, Vigsnaes, Louise, additional, van den Broek, Tim J., additional, de Boer, Paulo, additional, Montijn, Roy C., additional, and van der Vossen, Jos M. B. M., additional

Published
2022
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21. The Skin and Nose Microbiome and Its Association with Filaggrin Gene Mutations in Pediatric Atopic Dermatitis

Author

Van Mierlo, Minke M.F., Pardo, Luba M., Fieten, Karin B., Van Den Broek, Tim J., Schuren, Frank H.J., Van Geel, Michel, Pasmans, Suzanne G.M.A., Van Mierlo, Minke M.F., Pardo, Luba M., Fieten, Karin B., Van Den Broek, Tim J., Schuren, Frank H.J., Van Geel, Michel, and Pasmans, Suzanne G.M.A.

Abstract

Background: Interactions between the skin barrier, immune system, and microbiome underlie the development of atopic dermatitis (AD). Objective: To investigate the skin and nasal microbiome in relation to filaggrin gene (FLG) mutations. Methods: A cross-sectional study including 77 children with difficult-to-treat AD. The entire encoding region of FLG was screened for mutations using single molecule molecular inversion probes and next-generation sequencing. Bacterial swabs from the anterior nares, lesional and nonlesional skin were analyzed using 16S rRNA sequencing. For skin samples, additional qPCR was performed for Staphylococcus aureus and Staphylococcus epidermidis. Results: The prevalence of patients with a mutation in FLG was 40%, including 10 different mutations. Analyzing bacterial swabs from all three niches showed a significant effect for both niche and FLG mutation status on the overall microbiome composition. Using a subset analysis to test the effect of FLG mutation status per niche separately did not show a significant association to the microbiome. Shannon diversity and S. aureus abundance were significantly affected by the niche, but not by the presence of an FLG mutation. Conclusions: Our results suggest only a minor role for FLG mutation status on the overall microbiome, which is rather caused by differences in the present genera than by microbe richness and evenness.

Published
2022

24. A Machine Learning Algorithm for Quantitatively Diagnosing Oxidative Stress Risks in Healthy Adult Individuals Based on Health Space Methodology: A Proof-of-Concept Study Using Korean Cross-Sectional Cohort Data

Author

Kim, Youjin, primary, Kim, Yunsoo, additional, Hwang, Jiyoung, additional, van den Broek, Tim J., additional, Oh, Bumjo, additional, Kim, Ji Yeon, additional, Wopereis, Suzan, additional, Bouwman, Jildau, additional, and Kwon, Oran, additional

Published
2021
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25. A Novel Personalized Systems Nutrition Program Improves Dietary Patterns, Lifestyle Behaviors and Health-Related Outcomes: Results from the Habit Study

Author

de Hoogh, Iris M., primary, Winters, Barbara L., additional, Nieman, Kristin M., additional, Bijlsma, Sabina, additional, Krone, Tanja, additional, van den Broek, Tim J., additional, Anderson, Barbara D., additional, Caspers, Martien P. M., additional, Anthony, Joshua C., additional, and Wopereis, Suzan, additional

Published
2021
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27. The influence of treatment in alpine and moderate maritime climate on the composition of the skin microbiome in patients with difficult to treat atopic dermatitis

Author

van Mierlo, Minke M F, Totté, Joan E E, Fieten, Karin B, van den Broek, Tim J, Schuren, Frank H J, Pardo, Luba M, Pasmans, Suzanne G M A, University of Zurich, and van Mierlo, Minke M F

Subjects
2403 Immunology, 10183 Swiss Institute of Allergy and Asthma Research, 2723 Immunology and Allergy, 610 Medicine & health
Published
2019
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31. A network-based approach for identifying suitable biomarkers for oral immunotherapy of food allergy

Author

Van Bilsen, Jolanda H.M., Verschuren, Lars, Wagenaar, Laura, Vonk, Marlotte M., Van Esch, Betty C.A.M., Knippels, Léon M.J., Garssen, Johan, Smit, Joost J., Pieters, Raymond H.H., Van Den Broek, Tim J., Van Bilsen, Jolanda H.M., Verschuren, Lars, Wagenaar, Laura, Vonk, Marlotte M., Van Esch, Betty C.A.M., Knippels, Léon M.J., Garssen, Johan, Smit, Joost J., Pieters, Raymond H.H., and Van Den Broek, Tim J.

Abstract

Background Oral immunotherapy (OIT) is a promising therapeutic approach to treat food allergic patients. However, concerns with regards to safety and long-term efficacy of OIT remain. There is a need to identify biomarkers that predict, monitor and/or evaluate the effects of OIT. Here we present a method to select candidate biomarkers for efficacy and safety assessment of OIT using the computational approaches Bayesian networks (BN) and Topological Data Analysis (TDA). Results Data were used from fructo-oligosaccharide diet-supported OIT experiments performed in 3 independent cow’s milk allergy (CMA) and 2 independent peanut allergy (PNA) experiments in mice. Bioinformatical approaches were used to understand the data structure. The BN predicted the efficacy of OIT in the CMA with 86% and indicated a clear effect of scFOS/lcFOS on allergy parameters. For the PNA model, this BN (trained on CMA data) predicted an efficacy of OIT with 76% accuracy and shows similar effects of the allergen, treatment and diet as compared to the CMA model. The TDA identified clusters of biomarkers closely linked to biologically relevant clinical symptoms and also unrelated and redundant parameters within the network. Conclusions Here we provide a promising application of computational approaches to a) compare mechanistic features of two different food allergies during OIT b) determine the biological relevance of candidate biomarkers c) generate new hypotheses to explain why CMA has a different disease pattern than PNA and d) select relevant biomarkers for future studies.

Published
2019

32. A network-based approach for identifying suitable biomarkers for oral immunotherapy of food allergy

Author

Sub IRAS Tox ITX (immunotoxicologie), Afd Pharmacology, One Health Toxicologie, dIRAS RA-1, Pharmacology, Van Bilsen, Jolanda H.M., Verschuren, Lars, Wagenaar, Laura, Vonk, Marlotte M., Van Esch, Betty C.A.M., Knippels, Léon M.J., Garssen, Johan, Smit, Joost J., Pieters, Raymond H.H., Van Den Broek, Tim J., Sub IRAS Tox ITX (immunotoxicologie), Afd Pharmacology, One Health Toxicologie, dIRAS RA-1, Pharmacology, Van Bilsen, Jolanda H.M., Verschuren, Lars, Wagenaar, Laura, Vonk, Marlotte M., Van Esch, Betty C.A.M., Knippels, Léon M.J., Garssen, Johan, Smit, Joost J., Pieters, Raymond H.H., and Van Den Broek, Tim J.

Published
2019

34. Uncovering a Predictive Molecular Signature for the Onset of NASH-Related Fibrosis in a Translational NASH Mouse Model

Author

Pathologie Groep Goldschmeding, Pathologie Pathologen staf, Circulatory Health, van Koppen, Arianne, Verschuren, Lars, van den Hoek, Anita M., Verheij, Joanne, Morrison, Martine C., Li, Kelvin, Nagabukuro, Hiroshi, Costessi, Adalberto, Caspers, Martien P.M., van den Broek, Tim J., Sagartz, John, Kluft, Cornelis, Beysen, Carine, Emson, Claire, van Gool, Alain J., Goldschmeding, Roel, Stoop, Reinout, Bobeldijk-Pastorova, Ivana, Turner, Scott M., Hanauer, Guido, Hanemaaijer, Roeland, Pathologie Groep Goldschmeding, Pathologie Pathologen staf, Circulatory Health, van Koppen, Arianne, Verschuren, Lars, van den Hoek, Anita M., Verheij, Joanne, Morrison, Martine C., Li, Kelvin, Nagabukuro, Hiroshi, Costessi, Adalberto, Caspers, Martien P.M., van den Broek, Tim J., Sagartz, John, Kluft, Cornelis, Beysen, Carine, Emson, Claire, van Gool, Alain J., Goldschmeding, Roel, Stoop, Reinout, Bobeldijk-Pastorova, Ivana, Turner, Scott M., Hanauer, Guido, and Hanemaaijer, Roeland

Published
2018

35. Determinants of postprandial plasma bile acid kinetics in human volunteers

Author

Fiamoncini, Jarlei, primary, Yiorkas, Andrianos M., additional, Gedrich, Kurt, additional, Rundle, Milena, additional, Alsters, Sanne I., additional, Roeselers, Guus, additional, van den Broek, Tim J., additional, Clavel, Thomas, additional, Lagkouvardos, Ilias, additional, Wopereis, Suzan, additional, Frost, Gary, additional, van Ommen, Ben, additional, Blakemore, Alexandra I., additional, and Daniel, Hannelore, additional

Published
2017
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36. The impact of micronutrient status on health: correlation network analysis to understand the role of micronutrients in metabolic-inflammatory processes regulating homeostasis and phenotypic flexibility

Author

van den Broek, Tim J., primary, Kremer, Bas H. A., additional, Marcondes Rezende, Marisa, additional, Hoevenaars, Femke P. M., additional, Weber, Peter, additional, Hoeller, Ulrich, additional, van Ommen, Ben, additional, and Wopereis, Suzan, additional

Published
2017
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37. The influence of treatment in alpine and moderate maritime climate on the composition of the skin microbiome in patients with difficult to treat atopic dermatitis.

Author

van Mierlo MMF, Totté JEE, Fieten KB, van den Broek TJ, Schuren FHJ, Pardo LM, and Pasmans SGMA

Subjects
Adolescent, Child, Female, Humans, Male, Climate, Dermatitis, Atopic microbiology, Dermatitis, Atopic therapy, Microbiota, Skin microbiology, Staphylococcus aureus, Staphylococcus epidermidis
Abstract

Background: The skin microbiome, characterized by an overgrowth of Staphylococcus aureus, plays an important role in the pathogenesis of atopic dermatitis (AD). Multidisciplinary treatment in alpine climate is known for its positive effect on disease severity in children with AD and can result in a different immune response compared with moderate maritime climate. However, the effect on the composition of the skin microbiome in AD is unknown., Objective: To determine the effect of treatment in alpine climate and moderate maritime climate on the microbiome for lesional and non-lesional skin in children with difficult to treat AD., Results: Alpine climate treatment led to a significant change in the microbiota on lesional skin, whereas no significant change was found after moderate maritime climate. On both lesional and non-lesional skin, we observed a significant increase in Shannon diversity and a significant decrease in both Staphylococcus abundance and S aureus load after alpine climate treatment. The decrease in S aureus was significantly larger on lesional skin following alpine climate treatment compared with moderate maritime climate treatment. Staphylococcus epidermidis load was stable over time., Conclusions and Clinical Relevance: Alpine climate treatment leads to significant changes in the composition of the skin microbiome in children with AD, mainly caused by a reduction in the Staphylococcus genus. This study shows new perspectives in the potential mode of action for therapies in AD., (© 2019 John Wiley & Sons Ltd.)

Published
2019
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