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6. Skin grafting with the modified Meek technique in the standing horse using full thickness skin: Evaluation of acceptance, wound contraction and wound closure in chronic wounds.

Author

Wilmink, Jacintha M. and van Weeren, P. René

Abstract

Background: The modified Meek technique is not commonly used in equine wound management, despite the consistent reliable and superior results compared with other grafting techniques. Major drawbacks are the need for specialised, expensive equipment and general anaesthesia. Objectives: To describe adjustments of the modified Meek technique enabling use in the standing horse without the need for the full equipment. This implied the use of full‐thickness skin grafts manually harvested from the pectoral area and manually cut into micrografts. Graft acceptance; healing progress; and final functional and cosmetic result were outcome parameters. Study design: Descriptive case series. Methods: Eight horses with traumatic wounds at the dorsal side of the carpus or tarsus, healing by second intention, were treated. Original wound areas and areas of graft acceptance and rejection were determined from post‐processing of digital photographs and percentage acceptance, wound contraction and epithelialisation were calculated. Results: The initial mean wound area was 55.4 cm2. Graft acceptance was 95.3 ± 2.5%. Wound closure was due to 46.0 ± 25.6% wound contraction and 54.0 ± 25.6% epithelialisation and resulted in 96.8 ± 1.9% reduction of the initial wound area 28.0 ± 8.5 days after grafting. The scar was flat, flexible and functional, usually with thin and regular hair growth. The adapted procedure was fast and efficient, with a learning curve for the increased manual work. Main limitations: Small study population. Conclusions: This adapted modified Meek technique can successfully be performed in the standing horse and obviates the need for the full expensive equipment and general anaesthesia. The acceptance of the full‐thickness grafts is excellent resulting in fast and satisfactory healing. [ABSTRACT FROM AUTHOR]

Published
2024
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7. Orthotopic equine study confirms the pivotal importance of structural reinforcement over the pre-culture of cartilage implants

Author

de Ruijter, Mylène, Diloksumpan, Paweena, Dokter, Inge, Brommer, Harold, Smit, Ineke H., Levato, Riccardo, van Weeren, P. René, Castilho, Miguel, Malda, Jos, de Ruijter, Mylène, Diloksumpan, Paweena, Dokter, Inge, Brommer, Harold, Smit, Ineke H., Levato, Riccardo, van Weeren, P. René, Castilho, Miguel, and Malda, Jos

Abstract

In articular cartilage (AC), the collagen arcades provide the tissue with its extraordinary mechanical properties. As these structures cannot be restored once damaged, functional restoration of AC defects remains a major challenge. We report that the use of a converged bioprinted, osteochondral implant, based on a gelatin methacryloyl cartilage phase, reinforced with precisely patterned melt electrowritten polycaprolactone micrometer-scale fibers in a zonal fashion, inspired by native collagen architecture, can provide long-term mechanically stable neo-tissue in an orthotopic large animal model. The design of this novel implant was achieved via state-of-the-art converging of extrusion-based ceramic printing, melt electrowriting, and extrusion-based bioprinting. Interestingly, the cell-free implants, used as a control in this study, showed abundant cell ingrowth and similar favorable results as the cell-containing implants. Our findings underscore the hypothesis that mechanical stability is more determining for the successful survival of the implant than the presence of cells and pre-cultured extracellular matrix. This observation is of great translational importance and highlights the aptness of advanced 3D (bio)fabrication technologies for functional tissue restoration in the harsh articular joint mechanical environment.

Published
2024

8. Osseous pathologic changes in the lumbar region of the equine vertebral column: A descriptive post-mortem study in three breeds

Author

Spoormakers, Tijn J.P., Veraa, Stefanie, Graat, E. A.M., van Weeren, P. René, Brommer, Harold, Spoormakers, Tijn J.P., Veraa, Stefanie, Graat, E. A.M., van Weeren, P. René, and Brommer, Harold

Abstract

Background: Data on equine lumbar pathology hardly exist in breeds other than Thoroughbreds. Objectives: To describe pathological changes of the osseous lumbar vertebral column in Warmblood horses, Shetland ponies and Konik horses. Study design: Descriptive post-mortem study. Methods: The lumbar vertebral columns of 34 Warmblood horses, 28 Shetland ponies, and 18 Konik horses were examined by computed tomography (CT). Osteoarthritis (OA) of articular processes (APJs), OA of intertransverse joints (ITJs), intervertebral disc (IVD) mineralisation, impingement of spinous (SPs) and transverse (TPs) processes and spondylosis were scored. Breed differences in prevalence and severity of pathologies were analysed by linear regression analysis and by calculating Spearman's rank correlation coefficients (rs). Results: In Warmblood horses, the prevalence of OA of APJs, impingement of SPs and TPs was respectively 90%, 36%, and 35%, significantly higher than in the other breeds (p < 0.001). In Konik horses, IVD mineralisation (40%) and spondylosis (10%) were more frequent than in Warmbloods and Shetland ponies (p = 0.03). Severity score for OA of ITJs was highest in Shetland ponies (p < 0.001). For impingement of SPs, severity score was highest in Warmbloods (p = 0.03), and of TPs lowest in Shetland ponies (p = 0.003). For all parameters, except for spondylosis in Shetland ponies, there was a positive correlation between percentage of vertebrae affected and age, with IVD mineralisation scores increasing faster in Konik horses (p < 0.001). In all breeds, there was also a positive relation between scores of severity and age for OA of APJs and ITJs and for IVD mineralisation, with severity scores increasing faster in Shetland ponies (p = 0.04). Strong left/right correlations of the severity scores were seen for OA of the APJ, ITJ, impingement of TPs, and paramedian spondylosis (rs = 0.74–0.86, all p < 0.001). Main limitations: Clinical histories

Published
2024

9. Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in equine osteoarthritis: An exploratory 'multi-omics' study to identify composite biomarkers

Author

IOV Facs, Dep Clinical Sciences, Equine Musculoskeletal Biology, CS_Locomotion, Veterinaire biochemie, Celbiologie, Cell Biology, Metabolism & Cancer - Cellbiology, Cell Biology, Metabolism & Cancer - Metabolomics, Research Department OH - Biomolecular Health Sciences, Locomotion - Equine Sciences, Clarke, Emily, Varela, Laura, Jenkins, Rosalind E, Lozano-Andrés, Estefanía, Cywińska, Anna, Przewozny, Maciej, van Weeren, P René, van de Lest, Chris H A, Peffers, Mandy, Wauben, Marca H M, IOV Facs, Dep Clinical Sciences, Equine Musculoskeletal Biology, CS_Locomotion, Veterinaire biochemie, Celbiologie, Cell Biology, Metabolism & Cancer - Cellbiology, Cell Biology, Metabolism & Cancer - Metabolomics, Research Department OH - Biomolecular Health Sciences, Locomotion - Equine Sciences, Clarke, Emily, Varela, Laura, Jenkins, Rosalind E, Lozano-Andrés, Estefanía, Cywińska, Anna, Przewozny, Maciej, van Weeren, P René, van de Lest, Chris H A, Peffers, Mandy, and Wauben, Marca H M

Published
2024

10. Orthotopic equine study confirms the pivotal importance of structural reinforcement over the pre-culture of cartilage implants

Author

Equine Musculoskeletal Biology, Heelkunde, CS_Locomotion, Dep Clinical Sciences, Locomotion - Equine Sciences, de Ruijter, Mylène, Diloksumpan, Paweena, Dokter, Inge, Brommer, Harold, Smit, Ineke H., Levato, Riccardo, van Weeren, P. René, Castilho, Miguel, Malda, Jos, Equine Musculoskeletal Biology, Heelkunde, CS_Locomotion, Dep Clinical Sciences, Locomotion - Equine Sciences, de Ruijter, Mylène, Diloksumpan, Paweena, Dokter, Inge, Brommer, Harold, Smit, Ineke H., Levato, Riccardo, van Weeren, P. René, Castilho, Miguel, and Malda, Jos

Published
2024

11. Orthotopic equine study confirms the pivotal importance of structural reinforcement over the pre-culture of cartilage implants

Author

Orthopaedie Onderzoek, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, de Ruijter, Mylène, Diloksumpan, Paweena, Dokter, Inge, Brommer, Harold, Smit, Ineke H., Levato, Riccardo, van Weeren, P. René, Castilho, Miguel, Malda, Jos, Orthopaedie Onderzoek, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, de Ruijter, Mylène, Diloksumpan, Paweena, Dokter, Inge, Brommer, Harold, Smit, Ineke H., Levato, Riccardo, van Weeren, P. René, Castilho, Miguel, and Malda, Jos

Published
2024

13. Osseous pathologic changes in the lumbar region of the equine vertebral column: A descriptive post‐mortem study in three breeds.

Author

Spoormakers, Tijn J. P., Veraa, Stefanie, Graat, E. A. M., van Weeren, P. René, and Brommer, Harold

Abstract

Background: Data on equine lumbar pathology hardly exist in breeds other than Thoroughbreds. Objectives: To describe pathological changes of the osseous lumbar vertebral column in Warmblood horses, Shetland ponies and Konik horses. Study design: Descriptive post‐mortem study. Methods: The lumbar vertebral columns of 34 Warmblood horses, 28 Shetland ponies, and 18 Konik horses were examined by computed tomography (CT). Osteoarthritis (OA) of articular processes (APJs), OA of intertransverse joints (ITJs), intervertebral disc (IVD) mineralisation, impingement of spinous (SPs) and transverse (TPs) processes and spondylosis were scored. Breed differences in prevalence and severity of pathologies were analysed by linear regression analysis and by calculating Spearman's rank correlation coefficients (rs). Results: In Warmblood horses, the prevalence of OA of APJs, impingement of SPs and TPs was respectively 90%, 36%, and 35%, significantly higher than in the other breeds (p < 0.001). In Konik horses, IVD mineralisation (40%) and spondylosis (10%) were more frequent than in Warmbloods and Shetland ponies (p = 0.03). Severity score for OA of ITJs was highest in Shetland ponies (p < 0.001). For impingement of SPs, severity score was highest in Warmbloods (p = 0.03), and of TPs lowest in Shetland ponies (p = 0.003). For all parameters, except for spondylosis in Shetland ponies, there was a positive correlation between percentage of vertebrae affected and age, with IVD mineralisation scores increasing faster in Konik horses (p < 0.001). In all breeds, there was also a positive relation between scores of severity and age for OA of APJs and ITJs and for IVD mineralisation, with severity scores increasing faster in Shetland ponies (p = 0.04). Strong left/right correlations of the severity scores were seen for OA of the APJ, ITJ, impingement of TPs, and paramedian spondylosis (rs = 0.74–0.86, all p < 0.001). Main limitations: Clinical histories were not available. Conclusions: There are distinct breed differences between prevalence and severity of osseous pathologies of the lumbar spine. Warmblood horses have higher scores for most pathologies with IVD mineralisation being more important in Konik horses and OA of ITJs in Shetland ponies. [ABSTRACT FROM AUTHOR]

Published
2024
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15. Lipidome profiling of human and equine neutrophil-derived extracellular vesicles and their potential contribution to the ensemble of synovial fluid-derived extracellular vesicles during joint inflammation

Author

Varela, Laura, primary, Mol, Sanne, additional, Taanman-Kueter, Esther W., additional, Ryan, Sarah E., additional, Taams, Leonie S., additional, de Jong, Esther, additional, van Weeren, P. René, additional, van de Lest, Chris H.A., additional, and Wauben, Marca H.M., additional

Published
2023
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20. Proteome and phospholipidome interrelationship of synovial fluid-derived extracellular vesicles in equine osteoarthritis: An exploratory ‘multi-omics’ study to identify composite biomarkers

Author

Clarke, Emily J, primary, Varela, Laura, additional, Jenkins, Rosalind E, additional, Lozano−Andrés, Estefanía, additional, Cywińska, Anna, additional, Przewozny, Maciej, additional, van Weeren, P. René, additional, van de Lest, Chris H.A., additional, Peffers, Mandy, additional, and Wauben, Marca H.M., additional

Published
2023
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24. Contributors

Author

Aleman, Monica, primary, Annear, Matthew J., additional, Auer, Jörg A., additional, Bailey, Jeremy V., additional, Bartoe, Joshua T., additional, Barton, Michelle Henry, additional, Bettschart-Wolfensberger, Regula, additional, Bischofberger, Andrea S., additional, Blikslager, Anthony T., additional, Boone, Lindsey, additional, Bramlage, Larry R., additional, Carmalt, James L., additional, Carr, Elizabeth A., additional, Chalmers, Heather J., additional, Cheetham, Jonathan, additional, Cook, Vanessa L., additional, Davidson, Elizabeth J., additional, Davis, Jennifer L., additional, Disegi, John A., additional, Dixon, Padraic M., additional, Driessen, Bernd, additional, Duan, Wei, additional, Ducharme, Norm G., additional, Fogle, Callie, additional, Fortier, Lisa A., additional, Fowlie, Jennifer G., additional, Franklin, Samantha H., additional, Freeman, David E., additional, Frisbie, David D., additional, Fubini, Susan L., additional, Fürst, Anton E., additional, Gerard, Mathew P., additional, Glass, Kati G., additional, Hawkins, Jan F., additional, Hendrickson, Dean A., additional, Jackson, Michelle A., additional, Johnson, Sherry A., additional, Kidd, Jessica A., additional, Kümmerle, Jan M., additional, Lischer, Christoph J., additional, Lopez, Mandi J., additional, Love, Emma J., additional, Lugo, Joel, additional, MacKay, Robert J., additional, Mama, Khursheed R., additional, Marshall, John F., additional, Martens, Ann, additional, Mitchell, Katharyn, additional, Mowat, Freya M., additional, Mudge, Margaret C., additional, Munsterman, Amelia S., additional, Nelson, Nathan C., additional, Nickels, Frank A., additional, Nixon, Alan J., additional, O'Neill, Henry, additional, Ortved, Kyla F., additional, Pader, Karine, additional, Pease, Anthony P., additional, Peroni, John F., additional, Petersen-Jones, Simon M., additional, Pierce, Kenneth E., additional, Prange, Timo, additional, Provost, Patricia J., additional, Rakestraw, Peter C., additional, Richardson, Dean W., additional, Ringer, Simone K., additional, Rossignol, Fabrice, additional, Ruggles, Alan J., additional, Schnabel, Lauren V., additional, Schoster, Angelika, additional, Schott, Harold C., additional, Schramme, Michael, additional, Schumacher, James, additional, Schumacher, John, additional, Sherlock, Ceri, additional, Smith, Roger K.W., additional, Southwood, Louise L., additional, Stewart, Suzanne, additional, Theiss, Felix, additional, Tóth, Ferenc, additional, Townsend, Wendy M., additional, van Weeren, P. René, additional, Verwilghen, Denis, additional, Watkins, Jeffrey P., additional, Wilson, David A., additional, and Woodie, J. Brett, additional

Published
2019
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26. A Translational Model for Repeated Episodes of Joint Inflammation: Welfare, Clinical and Synovial Fluid Biomarker Assessment

Author

Kearney, Clodagh M, Korthagen, Nicoline M, Plomp, Saskia G M, Labberté, Margot C, de Grauw, Janny C, van Weeren, P René, Brama, Pieter A J, Kearney, Clodagh M, Korthagen, Nicoline M, Plomp, Saskia G M, Labberté, Margot C, de Grauw, Janny C, van Weeren, P René, and Brama, Pieter A J

Abstract

This study investigates repeated low-dose lipopolysaccharide (LPS) injections in equine joints as a model for recurrent joint inflammation and its impact on animal welfare. Joint inflammation was induced in eight horses by injecting 0.25 ng of LPS three times at two-week intervals. Welfare scores and clinical parameters were recorded at baseline and over 168 h post-injection. Serial synoviocentesis was performed for the analysis of a panel of synovial fluid biomarkers of inflammation and cartilage turnover. Clinical parameters and a final synoviocentesis were also performed eight weeks after the last sampling point to assess the recovery of normal joint homeostasis. Statistical methods were used to compare the magnitude of response to each of the 3 LPS inductions and to compare the baseline and final measurements. Each LPS injection produced consistent clinical and biomarker responses, with minimal changes in welfare scores. General matrix metalloproteinase (MMP) activity and joint circumference showed greater response to the second LPS induction, but response to the third was comparable to the first. Gylcosaminoglycans (GAG) levels showed a significantly decreased response with each induction, while collagen-cleavage neoepitope of type II collagen (C2C) and carboxypropetide of type II collagen epitope (CPII) showed quicker responses to the second and third inductions. All parameters were comparable to baseline values at the final timepoint. In conclusion, a consistent, reliable intra-articular inflammatory response can be achieved with repeated injections of 0.25 ng LPS, with minimal impact on animal welfare, suggesting potential as a refined translational model of recurrent joint inflammation.

Published
2023

27. Dual-contrast micro-CT enables cartilage lesion detection and tissue condition evaluation ex vivo

Author

Honkanen, Miitu K M, Mohammadi, Ali, Te Moller, Nikae C R, Ebrahimi, Mohammadhossein, Xu, Wujun, Plomp, Saskia, Pouran, Behdad, Lehto, Vesa-Pekka, Brommer, Harold, van Weeren, P René, Korhonen, Rami K, Töyräs, Juha, Mäkelä, Janne T A, Honkanen, Miitu K M, Mohammadi, Ali, Te Moller, Nikae C R, Ebrahimi, Mohammadhossein, Xu, Wujun, Plomp, Saskia, Pouran, Behdad, Lehto, Vesa-Pekka, Brommer, Harold, van Weeren, P René, Korhonen, Rami K, Töyräs, Juha, and Mäkelä, Janne T A

Abstract

Background: Post-traumatic osteoarthritis is a frequent joint disease in the horse. Currently, equine medicine lacks effective methods to diagnose the severity of chondral defects after an injury. Objectives: To investigate the capability of dual-contrast-enhanced computed tomography (dual-CECT) for detection of chondral lesions and evaluation of the severity of articular cartilage degeneration in the equine carpus ex vivo. Study design: Pre-clinical experimental study. Methods: In nine Shetland ponies, blunt and sharp grooves were randomly created (in vivo) in the cartilage of radiocarpal and middle carpal joints. The contralateral joint served as control. The ponies were subjected to an 8-week exercise protocol and euthanised 39 weeks after surgery. CECT scanning (ex vivo) of the joints was performed using a micro-CT scanner 1 hour after an intra-articular injection of a dual-contrast agent. The dual-contrast agent consisted of ioxaglate (negatively charged, q = −1) and bismuth nanoparticles (BiNPs, q = 0, diameter ≈ 0.2 µm). CECT results were compared to histological cartilage proteoglycan content maps acquired using digital densitometry. Results: BiNPs enabled prolonged visual detection of both groove types as they are too large to diffuse into the cartilage. Furthermore, proportional ioxaglate diffusion inside the tissue allowed differentiation between the lesion and ungrooved articular cartilage (3 mm from the lesion and contralateral joint). The mean ioxaglate partition in the lesion was 19 percentage points higher (P < 0.001) when compared with the contralateral joint. The digital densitometry and the dual-contrast CECT findings showed good subjective visual agreement. Main limitations: Ex vivo study protocol and a low number of investigated joints. Conclusions: The dual-CECT methodology, used in this study for the first time to image whole equine joints, is capable of effective lesion detection and simultaneous evaluation of the condition of the artic

Published
2023

28. Dual-contrast micro-CT enables cartilage lesion detection and tissue condition evaluation ex vivo

Author

Orthopaedie Onderzoek, Honkanen, Miitu K.M., Mohammadi, Ali, te Moller, Nikae C.R., Ebrahimi, Mohammadhossein, Xu, Wujun, Plomp, Saskia, Pouran, Behdad, Lehto, Vesa Pekka, Brommer, Harold, van Weeren, P. René, Korhonen, Rami K., Töyräs, Juha, Mäkelä, Janne T.A., Orthopaedie Onderzoek, Honkanen, Miitu K.M., Mohammadi, Ali, te Moller, Nikae C.R., Ebrahimi, Mohammadhossein, Xu, Wujun, Plomp, Saskia, Pouran, Behdad, Lehto, Vesa Pekka, Brommer, Harold, van Weeren, P. René, Korhonen, Rami K., Töyräs, Juha, and Mäkelä, Janne T.A.

Published
2023

29. Microstructural differences in the osteochondral unit of terrestrial and aquatic mammals

Author

Mancini, Irina A.D., Levato, Riccardo, Ksiezarczyk, Marlena M., Castilho, Miguel Dias, Chen, Michael, van Rijen, Mattie H.P., IJsseldijk, Lonneke L., Kik, Marja, van Weeren, P. René, Malda, Jos, Mancini, Irina A.D., Levato, Riccardo, Ksiezarczyk, Marlena M., Castilho, Miguel Dias, Chen, Michael, van Rijen, Mattie H.P., IJsseldijk, Lonneke L., Kik, Marja, van Weeren, P. René, and Malda, Jos

Abstract

During evolution, animals have returned from land to water, adapting with morpho-logical modifications to life in an aquatic environment. We compared the osteochondral units of the humeral head of marine and terrestrial mammals across species spanning a wide range of body weights, focusing on microstructural organization and biomechanical performance. Aquatic mammals feature cartilage with essentially random collagen fiber configuration, lacking the depth-dependent, arcade-like organization characteristic of terrestrial mammalian species. They have a less stiff articular cartilage at equilibrium with a significantly lower peak modulus, and at the osteochondral interface do not have a calcified cartilage layer, displaying only a thin, highly porous subchondral bone plate. This totally different constitution of the osteochondral unit in aquatic mammals reflects that accommodation of loading is the primordial function of the osteochondral unit. Recognizing the crucial importance of the microarchitecture-function relationship is pivotal for understanding articular biology and, hence, for the development of durable functional regenerative approaches for treatment of joint damage, which are thus far lacking.

Published
2023

30. A Translational Model for Repeated Episodes of Joint Inflammation: Welfare, Clinical and Synovial Fluid Biomarker Assessment

Author

Equine Musculoskeletal Biology, CS_Locomotion, Locomotion - Equine Sciences, Research Department VM - Clinical Sciences, Kearney, Clodagh M, Korthagen, Nicoline M, Plomp, Saskia G M, Labberté, Margot C, de Grauw, Janny C, van Weeren, P René, Brama, Pieter A J, Equine Musculoskeletal Biology, CS_Locomotion, Locomotion - Equine Sciences, Research Department VM - Clinical Sciences, Kearney, Clodagh M, Korthagen, Nicoline M, Plomp, Saskia G M, Labberté, Margot C, de Grauw, Janny C, van Weeren, P René, and Brama, Pieter A J

Published
2023

31. Acute joint inflammation induces a sharp increase in the number of synovial fluid EVs and modifies their phospholipid profile

Author

CS_Locomotion, Equine Musculoskeletal Biology, Veterinaire biochemie, Celbiologie, IOV Facs, Dep Clinical Sciences, Cell Biology, Metabolism & Cancer - Metabolomics, Cell Biology, Metabolism & Cancer - Cellbiology, Research Department OH - Biomolecular Health Sciences, Locomotion - Equine Sciences, Varela, Laura, van de Lest, Chris H A, Boere, Janneke, Libregts, Sten F W M, Lozano-Andrés, Estefania, van Weeren, P René, Wauben, Marca H M, CS_Locomotion, Equine Musculoskeletal Biology, Veterinaire biochemie, Celbiologie, IOV Facs, Dep Clinical Sciences, Cell Biology, Metabolism & Cancer - Metabolomics, Cell Biology, Metabolism & Cancer - Cellbiology, Research Department OH - Biomolecular Health Sciences, Locomotion - Equine Sciences, Varela, Laura, van de Lest, Chris H A, Boere, Janneke, Libregts, Sten F W M, Lozano-Andrés, Estefania, van Weeren, P René, and Wauben, Marca H M

Published
2023

32. Dual-contrast micro-CT enables cartilage lesion detection and tissue condition evaluation ex vivo

Author

Equine Musculoskeletal Biology, dES RMSC, Heelkunde, dES AVR, Dep Clinical Sciences, CS_Locomotion, Locomotion - Equine Sciences, Honkanen, Miitu K M, Mohammadi, Ali, Te Moller, Nikae C R, Ebrahimi, Mohammadhossein, Xu, Wujun, Plomp, Saskia, Pouran, Behdad, Lehto, Vesa-Pekka, Brommer, Harold, van Weeren, P René, Korhonen, Rami K, Töyräs, Juha, Mäkelä, Janne T A, Equine Musculoskeletal Biology, dES RMSC, Heelkunde, dES AVR, Dep Clinical Sciences, CS_Locomotion, Locomotion - Equine Sciences, Honkanen, Miitu K M, Mohammadi, Ali, Te Moller, Nikae C R, Ebrahimi, Mohammadhossein, Xu, Wujun, Plomp, Saskia, Pouran, Behdad, Lehto, Vesa-Pekka, Brommer, Harold, van Weeren, P René, Korhonen, Rami K, Töyräs, Juha, and Mäkelä, Janne T A

Published
2023

33. Microstructural differences in the osteochondral unit of terrestrial and aquatic mammals

Author

Orthopaedie Onderzoek, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, Mancini, Irina A.D., Levato, Riccardo, Ksiezarczyk, Marlena M., Castilho, Miguel Dias, Chen, Michael, van Rijen, Mattie H.P., Ijsseldijk, Lonneke L., Kik, Marja, van Weeren, P. René, Malda, Jos, Orthopaedie Onderzoek, Other research (not in main researchprogram), Regenerative Medicine and Stem Cells, Mancini, Irina A.D., Levato, Riccardo, Ksiezarczyk, Marlena M., Castilho, Miguel Dias, Chen, Michael, van Rijen, Mattie H.P., Ijsseldijk, Lonneke L., Kik, Marja, van Weeren, P. René, and Malda, Jos

Published
2023

34. Histological tissue healing following high-power laser treatment in a model of suspensory ligament branch injury

Author

Pluim, Mathilde, Heier, Annabelle, Plomp, Saskia, Boshuizen, Berit, Gröne, Andrea, van Weeren, P René, Vanderperren, Katrien, Martens, Ann, Dewulf, Jeroen, Chantziaras, Ilias, Koene, Marc, Luciani, Antonio, Oosterlinck, Maarten, Van Brantegem, Leen, Delesalle, Cathérine, Equine Musculoskeletal Biology, dES RMSC, LS Pharma, VP pathologie, dPB CR, dPB I&I, Dep Clinical Sciences, dES AVR, LS Equine Internal Medicine, CS_Locomotion, Equine Musculoskeletal Biology, dES RMSC, LS Pharma, VP pathologie, dPB CR, dPB I&I, Dep Clinical Sciences, dES AVR, LS Equine Internal Medicine, and CS_Locomotion

Subjects
collagen, tendon, TENDINOPATHY, LEVEL, INDUCED CORE LESIONS, HORSES, THERAPY, histology, ligament, Animals, Horses, Veterinary Sciences, ACHILLES TENDINITIS, Mammals, REPAIR, Factor VIII, Ligaments, TENDONS, Equine, General Medicine, IRRADIATION, horse, fibre, Eosine Yellowish-(YS), Horse Diseases, Joint Diseases
Abstract

Background High-power laser therapy gained popularity recently as a regenerative treatment for tendinitis and desmitis in the horse. However, studies evaluating the effects of laser therapy on tissue repair at the histological level in large mammals are lacking. Objectives To evaluate the effects of high-power laser therapy on suspensory desmitis healing, using a model of suspensory ligament branch injury. Study design In vivo experiments. Methods Standardised lesions were surgically induced in all four lateral suspensory branches of 12 healthy Warmblood horses. Laser therapy (class 4, 15W) was applied daily on two of four induced lesions for four consecutive weeks. Horses were randomly assigned to either short-term study (horses were sacrificed after 4 weeks) or long-term study (6 months). Suspensory ligament samples were scored after staining with haematoxylin-eosin and immunostaining for collagen 1- collagen 3- and factor VIII. Results In the short-term study, significantly better (lower) scores for variation in density (17% above cut-off score in treated lesions vs. 31% above cut-off score in controls, P = .03), shape of nuclei (54% vs 92%, P = .02), fibre alignment (32% vs 75%, P = .003) and fibre structure (38% vs 71%, P = .02) were found in laser-treated lesions when compared to controls. Collagen 3 expression was significantly higher (32% vs 19%, P = .006) in control lesions. In both short- and long-term studies combined, parameters lesion size (44% vs 56%, P = .02) and shape of nuclei (53% vs 84%, P = .05) scored significantly better in treated lesions. Long-term, significantly better (lower) scores were found in the laser-treated group for lesion size (15% vs 45%, P = .008) and a higher percentage above cut-off score for density of the nuclei (27% vs 9%, P = .02), compared to controls. Main limitations The model of suspensory branch injury is not an exact representation of clinical overstrain lesions. Conclusions These results suggest that high-power laser therapy enables better lesion healing than conservative treatment.

Published
2022

40. Folate Receptor Expression by Human Monocyte-Derived Macrophage Subtypes and Effects of Corticosteroids

Author

Chirurgie, dCSCA RMSC-1, Equine Musculoskeletal Biology, dES RMSC, Dep Clinical Sciences, CS_Locomotion, Warmink, Kelly, Siebelt, Michiel, Low, Philip S, Riemers, Frank M, Wang, Bingbing, Plomp, Saskia G M, Tryfonidou, Marianna A, van Weeren, P René, Weinans, Harrie, Korthagen, Nicoline M, Chirurgie, dCSCA RMSC-1, Equine Musculoskeletal Biology, dES RMSC, Dep Clinical Sciences, CS_Locomotion, Warmink, Kelly, Siebelt, Michiel, Low, Philip S, Riemers, Frank M, Wang, Bingbing, Plomp, Saskia G M, Tryfonidou, Marianna A, van Weeren, P René, Weinans, Harrie, and Korthagen, Nicoline M

Published
2022

41. Site- and Zone-Dependent Changes in Proteoglycan Content and Biomechanical Properties of Bluntly and Sharply Grooved Equine Articular Cartilage

Author

Equine Musculoskeletal Biology, dES RMSC, Heelkunde, dES AVR, Dep Clinical Sciences, CS_Locomotion, Mohammadi, Ali, Te Moller, Nikae C R, Ebrahimi, Mohammadhossein, Plomp, Saskia, Brommer, Harold, van Weeren, P René, Mäkelä, Janne T A, Töyräs, Juha, Korhonen, Rami K, Equine Musculoskeletal Biology, dES RMSC, Heelkunde, dES AVR, Dep Clinical Sciences, CS_Locomotion, Mohammadi, Ali, Te Moller, Nikae C R, Ebrahimi, Mohammadhossein, Plomp, Saskia, Brommer, Harold, van Weeren, P René, Mäkelä, Janne T A, Töyräs, Juha, and Korhonen, Rami K

Published
2022

42. Histological tissue healing following high-power laser treatment in a model of suspensory ligament branch injury

Author

Equine Musculoskeletal Biology, dES RMSC, LS Pharma, VP pathologie, dPB CR, dPB I&I, Dep Clinical Sciences, dES AVR, LS Equine Internal Medicine, CS_Locomotion, Pluim, Mathilde, Heier, Annabelle, Plomp, Saskia, Boshuizen, Berit, Gröne, Andrea, van Weeren, P René, Vanderperren, Katrien, Martens, Ann, Dewulf, Jeroen, Chantziaras, Ilias, Koene, Marc, Luciani, Antonio, Oosterlinck, Maarten, Van Brantegem, Leen, Delesalle, Cathérine, Equine Musculoskeletal Biology, dES RMSC, LS Pharma, VP pathologie, dPB CR, dPB I&I, Dep Clinical Sciences, dES AVR, LS Equine Internal Medicine, CS_Locomotion, Pluim, Mathilde, Heier, Annabelle, Plomp, Saskia, Boshuizen, Berit, Gröne, Andrea, van Weeren, P René, Vanderperren, Katrien, Martens, Ann, Dewulf, Jeroen, Chantziaras, Ilias, Koene, Marc, Luciani, Antonio, Oosterlinck, Maarten, Van Brantegem, Leen, and Delesalle, Cathérine

Published
2022

43. Folate Receptor Expression by Human Monocyte-Derived Macrophage Subtypes and Effects of Corticosteroids

Author

ORT Research, Regenerative Medicine and Stem Cells, Orthopaedie Onderzoek, Other research (not in main researchprogram), Warmink, Kelly, Siebelt, Michiel, Low, Philip S, Riemers, Frank M, Wang, Bingbing, Plomp, Saskia G M, Tryfonidou, Marianna A, van Weeren, P René, Weinans, Harrie, Korthagen, Nicoline M, ORT Research, Regenerative Medicine and Stem Cells, Orthopaedie Onderzoek, Other research (not in main researchprogram), Warmink, Kelly, Siebelt, Michiel, Low, Philip S, Riemers, Frank M, Wang, Bingbing, Plomp, Saskia G M, Tryfonidou, Marianna A, van Weeren, P René, Weinans, Harrie, and Korthagen, Nicoline M

Published
2022

49. Contributors

Author

Barrett, Myra F., primary, Brama, Pieter A.J., additional, de Grauw, Janny C., additional, Frisbie, David D., additional, Goodrich, Laurie R., additional, Haussler, Kevin K., additional, Kawcak, Christopher E., additional, King, Melissa R., additional, McIlwraith, C. Wayne, additional, Selberg, Kurt, additional, van Weeren, P. René, additional, and Werpy, Natasha M., additional

Published
2016
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