28 results on '"van Ineveld, Ravian"'
Search Results
2. Uncovering the mode of action of engineered T cells in patient cancer organoids
- Author
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Dekkers, Johanna F., Alieva, Maria, Cleven, Astrid, Keramati, Farid, Wezenaar, Amber K. L., van Vliet, Esmée J., Puschhof, Jens, Brazda, Peter, Johanna, Inez, Meringa, Angelo D., Rebel, Heggert G., Buchholz, Maj-Britt, Barrera Román, Mario, Zeeman, Amber L., de Blank, Sam, Fasci, Domenico, Geurts, Maarten H., Cornel, Annelisa M., Driehuis, Else, Millen, Rosemary, Straetemans, Trudy, Nicolasen, Mara J. T., Aarts-Riemens, Tineke, Ariese, Hendrikus C. R., Johnson, Hannah R., van Ineveld, Ravian L., Karaiskaki, Froso, Kopper, Oded, Bar-Ephraim, Yotam E., Kretzschmar, Kai, Eggermont, Alexander M. M., Nierkens, Stefan, Wehrens, Ellen J., Stunnenberg, Henk G., Clevers, Hans, Kuball, Jürgen, Sebestyen, Zsolt, and Rios, Anne C.
- Published
- 2023
- Full Text
- View/download PDF
3. Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D
- Author
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van Ineveld, Ravian L., Collot, Raphaël, Román, Mario Barrera, Pagliaro, Anna, Bessler, Nils, Ariese, Hendrikus C. R., Kleinnijenhuis, Michiel, Kool, Marcel, Alieva, Maria, Chuva de Sousa Lopes, Susana M., Wehrens, Ellen J., and Rios, Anne C.
- Published
- 2022
- Full Text
- View/download PDF
4. Single‐cell RNA sequencing of pediatric Hodgkin lymphoma to study the inhibition of T cell subtypes.
- Author
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de Kanter, Jurrian K., Steemers, Alexander S., Gonzalez, Daniel Montiel, van Ineveld, Ravian L., Blijleven, Catharina, Groenen, Niels, Trabut, Laurianne, Scheijde‐Vermeulen, Marijn A., Westera, Liset, Beishuizen, Auke, Rios, Anne C., Holstege, Frank C. P., Brandsma, Arianne M., Margaritis, Thanasis, van Boxtel, Ruben, and Meyer‐Wentrup, Friederike
- Published
- 2024
- Full Text
- View/download PDF
5. Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D
- Author
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van Ineveld, Ravian L., Kleinnijenhuis, Michiel, Alieva, Maria, de Blank, Sam, Barrera Roman, Mario, van Vliet, Esmée J., Martínez Mir, Clara, Johnson, Hannah R., Bos, Frank L., Heukers, Raimond, Chuva de Sousa Lopes, Susana M., Drost, Jarno, Dekkers, Johanna F., Wehrens, Ellen J., and Rios, Anne C.
- Published
- 2021
- Full Text
- View/download PDF
6. Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signalling
- Author
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Ponsioen, Bas, Post, Jasmin B., Buissant des Amorie, Julian R., Laskaris, Dimitrios, van Ineveld, Ravian L., Kersten, Simone, Bertotti, Andrea, Sassi, Francesco, Sipieter, François, Cappe, Benjamin, Mertens, Sander, Verlaan-Klink, Ingrid, Boj, Sylvia F., Vries, Rob G. J., Rehmann, Holger, Vandenabeele, Peter, Riquet, Franck B., Trusolino, Livio, Bos, Johannes L., and Snippert, Hugo J. G.
- Published
- 2021
- Full Text
- View/download PDF
7. 3D imaging for driving cancer discovery
- Author
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van Ineveld, Ravian L, van Vliet, Esmée J, Wehrens, Ellen J, Alieva, Maria, and Rios, Anne C
- Published
- 2022
- Full Text
- View/download PDF
8. An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
- Author
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Calandrini, Camilla, Schutgens, Frans, Oka, Rurika, Margaritis, Thanasis, Candelli, Tito, Mathijsen, Luka, Ammerlaan, Carola, van Ineveld, Ravian L., Derakhshan, Sepide, de Haan, Sanne, Dolman, Emmy, Lijnzaad, Philip, Custers, Lars, Begthel, Harry, Kerstens, Hindrik H. D., Visser, Lindy L., Rookmaaker, Maarten, Verhaar, Marianne, Tytgat, Godelieve A. M., Kemmeren, Patrick, de Krijger, Ronald R., Al-Saadi, Reem, Pritchard-Jones, Kathy, Kool, Marcel, Rios, Anne C., van den Heuvel-Eibrink, Marry M., Molenaar, Jan J., van Boxtel, Ruben, Holstege, Frank C. P., Clevers, Hans, and Drost, Jarno
- Published
- 2020
- Full Text
- View/download PDF
9. Uncovering the mode of action of engineered T cells in patient cancer organoids
- Author
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Dekkers, Johanna F, Alieva, Maria, Cleven, Astrid, Keramati, Farid, Wezenaar, Amber K L, van Vliet, Esmée J, Puschhof, Jens, Brazda, Peter, Johanna, Inez, Meringa, Angelo D, Rebel, Heggert G, Buchholz, Maj-Britt, Barrera Román, Mario, Zeeman, Amber L, de Blank, Sam, Fasci, Domenico, Geurts, Maarten H, Cornel, Annelisa M, Driehuis, Else, Millen, Rosemary, Straetemans, Trudy, Nicolasen, Mara J T, Aarts-Riemens, Tineke, Ariese, Hendrikus C R, Johnson, Hannah R, van Ineveld, Ravian L, Karaiskaki, Froso, Kopper, Oded, Bar-Ephraim, Yotam E, Kretzschmar, Kai, Eggermont, Alexander M M, Nierkens, Stefan, Wehrens, Ellen J, Stunnenberg, Henk G, Clevers, Hans, Kuball, Jürgen, Sebestyen, Zsolt, Rios, Anne C, Dekkers, Johanna F, Alieva, Maria, Cleven, Astrid, Keramati, Farid, Wezenaar, Amber K L, van Vliet, Esmée J, Puschhof, Jens, Brazda, Peter, Johanna, Inez, Meringa, Angelo D, Rebel, Heggert G, Buchholz, Maj-Britt, Barrera Román, Mario, Zeeman, Amber L, de Blank, Sam, Fasci, Domenico, Geurts, Maarten H, Cornel, Annelisa M, Driehuis, Else, Millen, Rosemary, Straetemans, Trudy, Nicolasen, Mara J T, Aarts-Riemens, Tineke, Ariese, Hendrikus C R, Johnson, Hannah R, van Ineveld, Ravian L, Karaiskaki, Froso, Kopper, Oded, Bar-Ephraim, Yotam E, Kretzschmar, Kai, Eggermont, Alexander M M, Nierkens, Stefan, Wehrens, Ellen J, Stunnenberg, Henk G, Clevers, Hans, Kuball, Jürgen, Sebestyen, Zsolt, and Rios, Anne C
- Abstract
Extending the success of cellular immunotherapies against blood cancers to the realm of solid tumors will require improved in vitro models that reveal therapeutic modes of action at the molecular level. Here we describe a system, called BEHAV3D, developed to study the dynamic interactions of immune cells and patient cancer organoids by means of imaging and transcriptomics. We apply BEHAV3D to live-track >150,000 engineered T cells cultured with patient-derived, solid-tumor organoids, identifying a 'super engager' behavioral cluster comprising T cells with potent serial killing capacity. Among other T cell concepts we also study cancer metabolome-sensing engineered T cells (TEGs) and detect behavior-specific gene signatures that include a group of 27 genes with no previously described T cell function that are expressed by super engager killer TEGs. We further show that type I interferon can prime resistant organoids for TEG-mediated killing. BEHAV3D is a promising tool for the characterization of behavioral-phenotypic heterogeneity of cellular immunotherapies and may support the optimization of personalized solid-tumor-targeting cell therapies.
- Published
- 2023
10. Uncovering the mode of action of engineered T cells in patient cancer organoids
- Author
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CTI, CTI Sebestyen, Infection & Immunity, Cancer, Hematologie ICAT, CTI Nierkens, MS Hematologie, CTI Straetemans, CTI Beringer, CDL Patiëntenzorg MI, CTI Research, CMM, Hubrecht Institute with UMC, CTI Kuball, Regenerative Medicine and Stem Cells, Dekkers, Johanna F., Alieva, Maria, Cleven, Astrid, Keramati, Farid, Wezenaar, Amber K.L., van Vliet, Esmée J., Puschhof, Jens, Brazda, Peter, Johanna, Inez, Meringa, Angelo D., Rebel, Heggert G., Buchholz, Maj Britt, Barrera Román, Mario, Zeeman, Amber L., de Blank, Sam, Fasci, Domenico, Geurts, Maarten H., Cornel, Annelisa M., Driehuis, Else, Millen, Rosemary, Straetemans, Trudy, Nicolasen, Mara J.T., Aarts-Riemens, Tineke, Ariese, Hendrikus C.R., Johnson, Hannah R., van Ineveld, Ravian L., Karaiskaki, Froso, Kopper, Oded, Bar-Ephraim, Yotam E., Kretzschmar, Kai, Eggermont, Alexander M.M., Nierkens, Stefan, Wehrens, Ellen J., Stunnenberg, Henk G., Clevers, Hans, Kuball, Jürgen, Sebestyen, Zsolt, Rios, Anne C., CTI, CTI Sebestyen, Infection & Immunity, Cancer, Hematologie ICAT, CTI Nierkens, MS Hematologie, CTI Straetemans, CTI Beringer, CDL Patiëntenzorg MI, CTI Research, CMM, Hubrecht Institute with UMC, CTI Kuball, Regenerative Medicine and Stem Cells, Dekkers, Johanna F., Alieva, Maria, Cleven, Astrid, Keramati, Farid, Wezenaar, Amber K.L., van Vliet, Esmée J., Puschhof, Jens, Brazda, Peter, Johanna, Inez, Meringa, Angelo D., Rebel, Heggert G., Buchholz, Maj Britt, Barrera Román, Mario, Zeeman, Amber L., de Blank, Sam, Fasci, Domenico, Geurts, Maarten H., Cornel, Annelisa M., Driehuis, Else, Millen, Rosemary, Straetemans, Trudy, Nicolasen, Mara J.T., Aarts-Riemens, Tineke, Ariese, Hendrikus C.R., Johnson, Hannah R., van Ineveld, Ravian L., Karaiskaki, Froso, Kopper, Oded, Bar-Ephraim, Yotam E., Kretzschmar, Kai, Eggermont, Alexander M.M., Nierkens, Stefan, Wehrens, Ellen J., Stunnenberg, Henk G., Clevers, Hans, Kuball, Jürgen, Sebestyen, Zsolt, and Rios, Anne C.
- Published
- 2023
11. Rapid tissue prototyping with micro-organospheres
- Author
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Wang, Zhaohui, primary, Boretto, Matteo, additional, Millen, Rosemary, additional, Natesh, Naveen, additional, Reckzeh, Elena S., additional, Hsu, Carolyn, additional, Negrete, Marcos, additional, Yao, Haipei, additional, Quayle, William, additional, Heaton, Brook E., additional, Harding, Alfred T., additional, Bose, Shree, additional, Driehuis, Else, additional, Beumer, Joep, additional, Rivera, Grecia O., additional, van Ineveld, Ravian L., additional, Gex, Donald, additional, DeVilla, Jessica, additional, Wang, Daisong, additional, Puschhof, Jens, additional, Geurts, Maarten H., additional, Yeung, Athena, additional, Hamele, Cait, additional, Smith, Amber, additional, Bankaitis, Eric, additional, Xiang, Kun, additional, Ding, Shengli, additional, Nelson, Daniel, additional, Delubac, Daniel, additional, Rios, Anne, additional, Abi-Hachem, Ralph, additional, Jang, David, additional, Goldstein, Bradley J., additional, Glass, Carolyn, additional, Heaton, Nicholas S., additional, Hsu, David, additional, Clevers, Hans, additional, and Shen, Xiling, additional
- Published
- 2022
- Full Text
- View/download PDF
12. Uncovering the mode of action of engineered T cells in patient cancer organoids
- Author
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Dekkers, Johanna F., primary, Alieva, Maria, additional, Cleven, Astrid, additional, Keramati, Farid, additional, Wezenaar, Amber K. L., additional, van Vliet, Esmée J., additional, Puschhof, Jens, additional, Brazda, Peter, additional, Johanna, Inez, additional, Meringa, Angelo D., additional, Rebel, Heggert G., additional, Buchholz, Maj-Britt, additional, Barrera Román, Mario, additional, Zeeman, Amber L., additional, de Blank, Sam, additional, Fasci, Domenico, additional, Geurts, Maarten H., additional, Cornel, Annelisa M., additional, Driehuis, Else, additional, Millen, Rosemary, additional, Straetemans, Trudy, additional, Nicolasen, Mara J. T., additional, Aarts-Riemens, Tineke, additional, Ariese, Hendrikus C. R., additional, Johnson, Hannah R., additional, van Ineveld, Ravian L., additional, Karaiskaki, Froso, additional, Kopper, Oded, additional, Bar-Ephraim, Yotam E., additional, Kretzschmar, Kai, additional, Eggermont, Alexander M. M., additional, Nierkens, Stefan, additional, Wehrens, Ellen J., additional, Stunnenberg, Henk G., additional, Clevers, Hans, additional, Kuball, Jürgen, additional, Sebestyen, Zsolt, additional, and Rios, Anne C., additional
- Published
- 2022
- Full Text
- View/download PDF
13. Uncovering the mode of action of engineered T cells in patient cancer organoids
- Author
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Dekkers, Johanna F, Alieva, Maria, Cleven, Astrid, Keramati, Farid, Wezenaar, Amber K L, van Vliet, Esmée J, Puschhof, Jens, Brazda, Peter, Johanna, Inez, Meringa, Angelo D, Rebel, Heggert G, Buchholz, Maj-Britt, Barrera Román, Mario, Zeeman, Amber L, de Blank, Sam, Fasci, Domenico, Geurts, Maarten H, Cornel, Annelisa M, Driehuis, Else, Millen, Rosemary, Straetemans, Trudy, Nicolasen, Mara J T, Aarts-Riemens, Tineke, Ariese, Hendrikus C R, Johnson, Hannah R, van Ineveld, Ravian L, Karaiskaki, Froso, Kopper, Oded, Bar-Ephraim, Yotam E, Kretzschmar, Kai, Eggermont, Alexander M M, Nierkens, Stefan, Wehrens, Ellen J, Stunnenberg, Henk G, Clevers, Hans, Kuball, Jürgen, Sebestyen, Zsolt, Rios, Anne C, Dekkers, Johanna F, Alieva, Maria, Cleven, Astrid, Keramati, Farid, Wezenaar, Amber K L, van Vliet, Esmée J, Puschhof, Jens, Brazda, Peter, Johanna, Inez, Meringa, Angelo D, Rebel, Heggert G, Buchholz, Maj-Britt, Barrera Román, Mario, Zeeman, Amber L, de Blank, Sam, Fasci, Domenico, Geurts, Maarten H, Cornel, Annelisa M, Driehuis, Else, Millen, Rosemary, Straetemans, Trudy, Nicolasen, Mara J T, Aarts-Riemens, Tineke, Ariese, Hendrikus C R, Johnson, Hannah R, van Ineveld, Ravian L, Karaiskaki, Froso, Kopper, Oded, Bar-Ephraim, Yotam E, Kretzschmar, Kai, Eggermont, Alexander M M, Nierkens, Stefan, Wehrens, Ellen J, Stunnenberg, Henk G, Clevers, Hans, Kuball, Jürgen, Sebestyen, Zsolt, and Rios, Anne C
- Abstract
Extending the success of cellular immunotherapies against blood cancers to the realm of solid tumors will require improved in vitro models that reveal therapeutic modes of action at the molecular level. Here we describe a system, called BEHAV3D, developed to study the dynamic interactions of immune cells and patient cancer organoids by means of imaging and transcriptomics. We apply BEHAV3D to live-track >150,000 engineered T cells cultured with patient-derived, solid-tumor organoids, identifying a 'super engager' behavioral cluster comprising T cells with potent serial killing capacity. Among other T cell concepts we also study cancer metabolome-sensing engineered T cells (TEGs) and detect behavior-specific gene signatures that include a group of 27 genes with no previously described T cell function that are expressed by super engager killer TEGs. We further show that type I interferon can prime resistant organoids for TEG-mediated killing. BEHAV3D is a promising tool for the characterization of behavioral-phenotypic heterogeneity of cellular immunotherapies and may support the optimization of personalized solid-tumor-targeting cell therapies.
- Published
- 2022
14. Rapid tissue prototyping with micro-organospheres
- Author
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Wang, Zhaohui, Boretto, Matteo, Millen, Rosemary, Natesh, Naveen, Reckzeh, Elena S, Hsu, Carolyn, Negrete, Marcos, Yao, Haipei, Quayle, William, Heaton, Brook E, Harding, Alfred T, Bose, Shree, Driehuis, Else, Beumer, Joep, Rivera, Grecia O, van Ineveld, Ravian L, Gex, Donald, DeVilla, Jessica, Wang, Daisong, Puschhof, Jens, Geurts, Maarten H, Yeung, Athena, Hamele, Cait, Smith, Amber, Bankaitis, Eric, Xiang, Kun, Ding, Shengli, Nelson, Daniel, Delubac, Daniel, Rios, Anne, Abi-Hachem, Ralph, Jang, David, Goldstein, Bradley J, Glass, Carolyn, Heaton, Nicholas S, Hsu, David, Clevers, Hans, Shen, Xiling, Wang, Zhaohui, Boretto, Matteo, Millen, Rosemary, Natesh, Naveen, Reckzeh, Elena S, Hsu, Carolyn, Negrete, Marcos, Yao, Haipei, Quayle, William, Heaton, Brook E, Harding, Alfred T, Bose, Shree, Driehuis, Else, Beumer, Joep, Rivera, Grecia O, van Ineveld, Ravian L, Gex, Donald, DeVilla, Jessica, Wang, Daisong, Puschhof, Jens, Geurts, Maarten H, Yeung, Athena, Hamele, Cait, Smith, Amber, Bankaitis, Eric, Xiang, Kun, Ding, Shengli, Nelson, Daniel, Delubac, Daniel, Rios, Anne, Abi-Hachem, Ralph, Jang, David, Goldstein, Bradley J, Glass, Carolyn, Heaton, Nicholas S, Hsu, David, Clevers, Hans, and Shen, Xiling
- Abstract
In vitro tissue models hold great promise for modeling diseases and drug responses. Here, we used emulsion microfluidics to form micro-organospheres (MOSs), which are droplet-encapsulated miniature three-dimensional (3D) tissue models that can be established rapidly from patient tissues or cells. MOSs retain key biological features and responses to chemo-, targeted, and radiation therapies compared with organoids. The small size and large surface-to-volume ratio of MOSs enable various applications including quantitative assessment of nutrient dependence, pathogen-host interaction for anti-viral drug screening, and a rapid potency assay for chimeric antigen receptor (CAR)-T therapy. An automated MOS imaging pipeline combined with machine learning overcomes plating variation, distinguishes tumorspheres from stroma, differentiates cytostatic versus cytotoxic drug effects, and captures resistant clones and heterogeneity in drug response. This pipeline is capable of robust assessments of drug response at individual-tumorsphere resolution and provides a rapid and high-throughput therapeutic profiling platform for precision medicine.
- Published
- 2022
15. Rapid tissue prototyping with micro-organospheres
- Author
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CMM, Cancer, Hubrecht Institute with UMC, Wang, Zhaohui, Boretto, Matteo, Millen, Rosemary, Natesh, Naveen, Reckzeh, Elena S., Hsu, Carolyn, Negrete, Marcos, Yao, Haipei, Quayle, William, Heaton, Brook E., Harding, Alfred T., Bose, Shree, Driehuis, Else, Beumer, Joep, Rivera, Grecia O., van Ineveld, Ravian L., Gex, Donald, DeVilla, Jessica, Wang, Daisong, Puschhof, Jens, Geurts, Maarten H., Yeung, Athena, Hamele, Cait, Smith, Amber, Bankaitis, Eric, Xiang, Kun, Ding, Shengli, Nelson, Daniel, Delubac, Daniel, Rios, Anne, Abi-Hachem, Ralph, Jang, David, Goldstein, Bradley J., Glass, Carolyn, Heaton, Nicholas S., Hsu, David, Clevers, Hans, Shen, Xiling, CMM, Cancer, Hubrecht Institute with UMC, Wang, Zhaohui, Boretto, Matteo, Millen, Rosemary, Natesh, Naveen, Reckzeh, Elena S., Hsu, Carolyn, Negrete, Marcos, Yao, Haipei, Quayle, William, Heaton, Brook E., Harding, Alfred T., Bose, Shree, Driehuis, Else, Beumer, Joep, Rivera, Grecia O., van Ineveld, Ravian L., Gex, Donald, DeVilla, Jessica, Wang, Daisong, Puschhof, Jens, Geurts, Maarten H., Yeung, Athena, Hamele, Cait, Smith, Amber, Bankaitis, Eric, Xiang, Kun, Ding, Shengli, Nelson, Daniel, Delubac, Daniel, Rios, Anne, Abi-Hachem, Ralph, Jang, David, Goldstein, Bradley J., Glass, Carolyn, Heaton, Nicholas S., Hsu, David, Clevers, Hans, and Shen, Xiling
- Published
- 2022
16. Snake Venom Gland Organoids
- Author
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Post, Yorick, Puschhof, Jens, Beumer, Joep, Kerkkamp, Harald M, de Bakker, Merijn A G, Slagboom, Julien, de Barbanson, Buys, Wevers, Nienke R, Spijkers, Xandor M, Olivier, Thomas, Kazandjian, Taline D, Ainsworth, Stuart, Iglesias, Carmen Lopez, van de Wetering, Willine J, Heinz, Maria C, van Ineveld, Ravian L, van Kleef, Regina G D M, Begthel, Harry, Korving, Jeroen, Bar-Ephraim, Yotam E, Getreuer, Walter, Rios, Anne C, Westerink, Remco H S, Snippert, Hugo J G, van Oudenaarden, Alexander, Peters, Peter J, Vonk, Freek J, Kool, Jeroen, Richardson, Michael K, Casewell, Nicholas R, Clevers, Hans, Landdegradatie en aardobservatie, One Health Toxicologie, dIRAS RA-1, Sub Developmental Biology, Dep Farmaceutische wetenschappen, Developmental Biology, Landdegradatie en aardobservatie, One Health Toxicologie, dIRAS RA-1, Sub Developmental Biology, Dep Farmaceutische wetenschappen, Developmental Biology, Faculteit FHML Centraal, Institute of Nanoscopy (IoN), RS: M4I - Nanoscopy, BioAnalytical Chemistry, AIMMS, and Hubrecht Institute for Developmental Biology and Stem Cell Research
- Subjects
Cell Culture Techniques ,Venom ,Stem cell marker ,Salivary Glands ,Transcriptome ,transcriptomics ,0302 clinical medicine ,0303 health sciences ,snake ,Stem Cells ,TERM PRIMARY CULTURE ,LGR5 ,Snakes ,knock-in reporter ,Cell biology ,Organoids ,RECEPTORS ,Adult Stem Cells ,Snake venom ,Stem cell ,STEM-CELLS ,Adult stem cell ,wd_410 ,Snake Venoms ,PROTEINS ,organoid ,qu_475 ,Coral Snakes ,Biology ,SECRETORY-CELLS ,complex mixtures ,General Biochemistry, Genetics and Molecular Biology ,Lgr5 ,03 medical and health sciences ,stem cells ,single cell RNA sequencing ,Organoid ,Animals ,SDG 14 - Life Below Water ,030304 developmental biology ,Toxins, Biological ,venom gland ,IDENTIFICATION ,Gene Expression Profiling ,qu_300 ,IN-VITRO ,NEUROTOXINS ,EVOLUTION ,qu_550 ,heterogeneity ,030217 neurology & neurosurgery - Abstract
Wnt dependency and Lgr5 expression define multiple mammalian epithelial stem cell types. Under defined growth factor conditions, such adult stem cells (ASCs) grow as 3D organoids that recapitulate essential features of the pertinent epithelium. Here, we establish long-term expanding venom gland organoids from several snake species. The newly assembled transcriptome of the Cape coral snake reveals that organoids express high levels of toxin transcripts. Single-cell RNA sequencing of both organoids and primary tissue identifies distinct venom-expressing cell types as well as proliferative cells expressing homologs of known mammalian stem cell markers. A hard-wired regional heterogeneity in the expression of individual venom components is maintained in organoid cultures. Harvested venom peptides reflect crude venom composition and display biological activity. This study extends organoid technology to reptilian tissues and describes an experimentally tractable model system representing the snake venom gland.
- Published
- 2020
17. LGR6 marks nephron progenitor cells
- Author
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Groep Holstege, Hubrecht Institute with UMC, van Ineveld, Ravian L., Margaritis, Thanasis, Kooiman, Berend A.P., Groenveld, Femke, Ariese, Hendrikus C.R., Lijnzaad, Philip, Johnson, Hannah R., Korving, Jeroen, Wehrens, Ellen J., Holstege, Frank, van Rheenen, Jacco, Drost, Jarno, Rios, Anne C., Bos, Frank L., Groep Holstege, Hubrecht Institute with UMC, van Ineveld, Ravian L., Margaritis, Thanasis, Kooiman, Berend A.P., Groenveld, Femke, Ariese, Hendrikus C.R., Lijnzaad, Philip, Johnson, Hannah R., Korving, Jeroen, Wehrens, Ellen J., Holstege, Frank, van Rheenen, Jacco, Drost, Jarno, Rios, Anne C., and Bos, Frank L.
- Published
- 2021
18. Quantifying single-cell ERK dynamics in colorectal cancer organoids reveals EGFR as an amplifier of oncogenic MAPK pathway signalling
- Author
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CMM Groep Snippert, Cancer, CMM Groep Bos, Hubrecht Institute with UMC, Ponsioen, Bas, Post, Jasmin B, Buissant des Amorie, Julian R, Laskaris, Dimitrios, van Ineveld, Ravian L, Kersten, Simone, Bertotti, Andrea, Sassi, Francesco, Sipieter, François, Cappe, Benjamin, Mertens, Sander, Verlaan-Klink, Ingrid, Boj, Sylvia F, Vries, Rob G J, Rehmann, Holger, Vandenabeele, Peter, Riquet, Franck B, Trusolino, Livio, Bos, Johannes L, Snippert, Hugo J G, CMM Groep Snippert, Cancer, CMM Groep Bos, Hubrecht Institute with UMC, Ponsioen, Bas, Post, Jasmin B, Buissant des Amorie, Julian R, Laskaris, Dimitrios, van Ineveld, Ravian L, Kersten, Simone, Bertotti, Andrea, Sassi, Francesco, Sipieter, François, Cappe, Benjamin, Mertens, Sander, Verlaan-Klink, Ingrid, Boj, Sylvia F, Vries, Rob G J, Rehmann, Holger, Vandenabeele, Peter, Riquet, Franck B, Trusolino, Livio, Bos, Johannes L, and Snippert, Hugo J G
- Published
- 2021
19. Behavioral-transcriptomic landscape of engineered T cells targeting human cancer organoids
- Author
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Dekkers, Johanna F., primary, Alieva, Maria, additional, Cleven, Astrid, additional, Keramati, Farid, additional, Brazda, Peter, additional, Rebel, Heggert G., additional, Wezenaar, Amber K.L., additional, Puschhof, Jens, additional, Buchholz, Maj-Britt, additional, Román, Mario Barrera, additional, Johanna, Inez, additional, Meringa, Angelo D., additional, Fasci, Domenico, additional, Geurts, Maarten H., additional, Ariese, Hendrikus C.R., additional, van Vliet, Esmée J., additional, van Ineveld, Ravian L., additional, Karaiskaki, Effrosyni, additional, Kopper, Oded, additional, Bar-Ephraim, Yotam E., additional, Kretzschmar, Kai, additional, Eggermont, Alexander M.M., additional, Wehrens, Ellen J., additional, Stunnenberg, Henk G., additional, Clevers, Hans, additional, Kuball, Jürgen, additional, Sebestyen, Zsolt, additional, and Rios, Anne C., additional
- Published
- 2021
- Full Text
- View/download PDF
20. An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
- Author
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UU BETA RESEARCH, Faculteit Diergeneeskunde, Calandrini, Camilla, Schutgens, Frans, Oka, Rurika, Margaritis, Thanasis, Candelli, Tito, Mathijsen, Luka, Ammerlaan, Carola, van Ineveld, Ravian L, Derakhshan, Sepide, de Haan, Sanne, Dolman, Emmy, Lijnzaad, Philip, Custers, Lars, Begthel, Harry, Kerstens, Hindrik H D, Visser, Lindy L, Rookmaaker, Maarten, Verhaar, Marianne, Tytgat, Godelieve A M, Kemmeren, Patrick, de Krijger, Ronald R, Al-Saadi, Reem, Pritchard-Jones, Kathy, Kool, Marcel, Rios, Anne C, van den Heuvel-Eibrink, Marry M, Molenaar, Jan J, van Boxtel, Ruben, Holstege, Frank C P, Clevers, Hans, Drost, Jarno, UU BETA RESEARCH, Faculteit Diergeneeskunde, Calandrini, Camilla, Schutgens, Frans, Oka, Rurika, Margaritis, Thanasis, Candelli, Tito, Mathijsen, Luka, Ammerlaan, Carola, van Ineveld, Ravian L, Derakhshan, Sepide, de Haan, Sanne, Dolman, Emmy, Lijnzaad, Philip, Custers, Lars, Begthel, Harry, Kerstens, Hindrik H D, Visser, Lindy L, Rookmaaker, Maarten, Verhaar, Marianne, Tytgat, Godelieve A M, Kemmeren, Patrick, de Krijger, Ronald R, Al-Saadi, Reem, Pritchard-Jones, Kathy, Kool, Marcel, Rios, Anne C, van den Heuvel-Eibrink, Marry M, Molenaar, Jan J, van Boxtel, Ruben, Holstege, Frank C P, Clevers, Hans, and Drost, Jarno
- Published
- 2020
21. Snake Venom Gland Organoids
- Author
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Landdegradatie en aardobservatie, One Health Toxicologie, dIRAS RA-1, Sub Developmental Biology, Dep Farmaceutische wetenschappen, Developmental Biology, Post, Yorick, Puschhof, Jens, Beumer, Joep, Kerkkamp, Harald M, de Bakker, Merijn A G, Slagboom, Julien, de Barbanson, Buys, Wevers, Nienke R, Spijkers, Xandor M, Olivier, Thomas, Kazandjian, Taline D, Ainsworth, Stuart, Iglesias, Carmen Lopez, van de Wetering, Willine J, Heinz, Maria C, van Ineveld, Ravian L, van Kleef, Regina G D M, Begthel, Harry, Korving, Jeroen, Bar-Ephraim, Yotam E, Getreuer, Walter, Rios, Anne C, Westerink, Remco H S, Snippert, Hugo J G, van Oudenaarden, Alexander, Peters, Peter J, Vonk, Freek J, Kool, Jeroen, Richardson, Michael K, Casewell, Nicholas R, Clevers, Hans, Landdegradatie en aardobservatie, One Health Toxicologie, dIRAS RA-1, Sub Developmental Biology, Dep Farmaceutische wetenschappen, Developmental Biology, Post, Yorick, Puschhof, Jens, Beumer, Joep, Kerkkamp, Harald M, de Bakker, Merijn A G, Slagboom, Julien, de Barbanson, Buys, Wevers, Nienke R, Spijkers, Xandor M, Olivier, Thomas, Kazandjian, Taline D, Ainsworth, Stuart, Iglesias, Carmen Lopez, van de Wetering, Willine J, Heinz, Maria C, van Ineveld, Ravian L, van Kleef, Regina G D M, Begthel, Harry, Korving, Jeroen, Bar-Ephraim, Yotam E, Getreuer, Walter, Rios, Anne C, Westerink, Remco H S, Snippert, Hugo J G, van Oudenaarden, Alexander, Peters, Peter J, Vonk, Freek J, Kool, Jeroen, Richardson, Michael K, Casewell, Nicholas R, and Clevers, Hans
- Published
- 2020
22. Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
- Author
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Hubrecht Institute with UMC, van Ineveld, Ravian L, Ariese, Hendrikus C R, Wehrens, Ellen J, Dekkers, Johanna F, Rios, Anne C, Hubrecht Institute with UMC, van Ineveld, Ravian L, Ariese, Hendrikus C R, Wehrens, Ellen J, Dekkers, Johanna F, and Rios, Anne C
- Published
- 2020
23. An organoid biobank for childhood kidney cancers that captures disease and tissue heterogeneity
- Author
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Nefro Vasculaire Geneeskunde, Groep Holstege, CMM Groep Klumperman, MS Nefrologie, Regenerative Medicine and Stem Cells, Circulatory Health, Pathologie Groep Blokx, Pathologie Pathologen staf, Child Health, CMM Groep Cuppen, Cancer, CMM Sectie Molecular Cancer Research, Hubrecht Institute with UMC, Calandrini, Camilla, Schutgens, Frans, Oka, Rurika, Margaritis, Thanasis, Candelli, Tito, Mathijsen, Luka, Ammerlaan, Carola, van Ineveld, Ravian L., Derakhshan, Sepide, de Haan, Sanne, Dolman, Emmy, Lijnzaad, Philip, Custers, Lars, Begthel, Harry, Kerstens, Hindrik H.D., Visser, Lindy L., Rookmaaker, Maarten, Verhaar, Marianne, Tytgat, Godelieve A.M., Kemmeren, Patrick, de Krijger, Ronald R., Al-Saadi, Reem, Pritchard-Jones, Kathy, Kool, Marcel, Rios, Anne C., van den Heuvel-Eibrink, Marry M., Molenaar, Jan J., van Boxtel, Ruben, Holstege, Frank C.P., Clevers, Hans, Drost, Jarno, Nefro Vasculaire Geneeskunde, Groep Holstege, CMM Groep Klumperman, MS Nefrologie, Regenerative Medicine and Stem Cells, Circulatory Health, Pathologie Groep Blokx, Pathologie Pathologen staf, Child Health, CMM Groep Cuppen, Cancer, CMM Sectie Molecular Cancer Research, Hubrecht Institute with UMC, Calandrini, Camilla, Schutgens, Frans, Oka, Rurika, Margaritis, Thanasis, Candelli, Tito, Mathijsen, Luka, Ammerlaan, Carola, van Ineveld, Ravian L., Derakhshan, Sepide, de Haan, Sanne, Dolman, Emmy, Lijnzaad, Philip, Custers, Lars, Begthel, Harry, Kerstens, Hindrik H.D., Visser, Lindy L., Rookmaaker, Maarten, Verhaar, Marianne, Tytgat, Godelieve A.M., Kemmeren, Patrick, de Krijger, Ronald R., Al-Saadi, Reem, Pritchard-Jones, Kathy, Kool, Marcel, Rios, Anne C., van den Heuvel-Eibrink, Marry M., Molenaar, Jan J., van Boxtel, Ruben, Holstege, Frank C.P., Clevers, Hans, and Drost, Jarno
- Published
- 2020
24. Abstract IA27: Patient-derived organoids in pediatric cancer research
- Author
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Calandrini, Camilla, primary, Schutgens, Frans, additional, Oka, Rurika, additional, Margaritis, Thanasis, additional, Candelli, Tito, additional, Mathijsen, Luka, additional, Ammerlaan, Carola, additional, van Ineveld, Ravian, additional, Derakhshan, Sepideh, additional, Custers, Lars, additional, Lijnzaad, Philip, additional, Begthel, Harry, additional, Kerstens, Hinri, additional, Rookmaker, Maarten, additional, Verhaar, Marianne, additional, Kemmeren, Patrick, additional, de Krijger, Ronald, additional, Pritchard-Jones, Kathy, additional, Rios, Anne, additional, van den Heuvel-Eibrink, Marry, additional, Holstege, Frank, additional, van Boxtel, Ruben, additional, Clevers, Hans, additional, and Drost, Jarno, additional
- Published
- 2020
- Full Text
- View/download PDF
25. Single-Cell Resolution Three-Dimensional Imaging of Intact Organoids
- Author
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van Ineveld, Ravian L., primary, Ariese, Hendrikus C.R., primary, Wehrens, Ellen J., primary, Dekkers, Johanna F., primary, and Rios, Anne C., primary
- Published
- 2020
- Full Text
- View/download PDF
26. LGR6 marks nephron progenitor cells.
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van Ineveld, Ravian L., Margaritis, Thanasis, Kooiman, Berend A.P., Groenveld, Femke, Ariese, Hendrikus C.R., Lijnzaad, Philip, Johnson, Hannah R., Korving, Jeroen, Wehrens, Ellen J., Holstege, Frank, van Rheenen, Jacco, Drost, Jarno, Rios, Anne C., and Bos, Frank L.
- Subjects
PROGENITOR cells ,KIDNEY tubules ,EPITHELIAL-mesenchymal transition ,NEPHRONS ,RNA sequencing - Abstract
Background: Nephron progenitor cells (NPCs) undergo a stepwise process to generate all mature nephron structures. Mesenchymal to epithelial transition (MET) is considered a multistep process of NPC differentiation to ensure progressive establishment of new nephrons. However, despite this important role, to date, no marker for NPCs undergoing MET in the nephron exists. Results: Here, we identify LGR6 as a NPC marker, expressed in very early cap mesenchyme, pre‐tubular aggregates, renal vesicles, and in segments of S‐shaped bodies, following the trajectory of MET. By using a lineage tracing approach in embryonic explants in combination with confocal imaging and single‐cell RNA sequencing, we provide evidence for the multiple fates of LGR6+ cells during embryonic nephrogenesis. Moreover, by using long‐term in vivo lineage tracing, we show that postnatal LGR6+ cells are capable of generating the multiple lineages of the nephrons. Conclusions: Given the profound early mesenchymal expression and MET signature of LGR6+ cells, together with the lineage tracing of mesenchymal LGR6+ cells, we conclude that LGR6+ cells contribute to all nephrogenic segments by undergoing MET. LGR6+ cells can therefore be considered an early committed NPC population during embryonic and postnatal nephrogenesis with potential regenerative capability. Key Findings: Lgr6 is expressed in the earliest cap mesenchyme pool, a niche where nephrogenic progenitor cells (NPCs) are found.Lgr6 marks NPCs undergoing mesenchymal to epithelial transition, following the main process of nephron development.Using ex vivo and vivo lineage tracing, we show that mesenchymal Lgr6 expressing cells give rise to multiple types of mesenchymal derived nephron segments, including specialized glomerular epithelium, such as podocytes. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
27. Intraclonal Plasticity in Mammary Tumors Revealed through Large-Scale Single-Cell Resolution 3D Imaging
- Author
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Rios, Anne C., primary, Capaldo, Bianca D., additional, Vaillant, François, additional, Pal, Bhupinder, additional, van Ineveld, Ravian, additional, Dawson, Caleb A., additional, Chen, Yunshun, additional, Nolan, Emma, additional, Fu, Nai Yang, additional, Jackling, Felicity C., additional, Devi, Sapna, additional, Clouston, David, additional, Whitehead, Lachlan, additional, Smyth, Gordon K., additional, Mueller, Scott N., additional, Lindeman, Geoffrey J., additional, and Visvader, Jane E., additional
- Published
- 2019
- Full Text
- View/download PDF
28. Cover Image.
- Author
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van Ineveld, Ravian L., Margaritis, Thanasis, Kooiman, Berend A.P., Groenveld, Femke, Ariese, Hendrikus C.R., Lijnzaad, Philip, Johnson, Hannah R., Korving, Jeroen, Wehrens, Ellen J., Holstege, Frank, van Rheenen, Jacco, Drost, Jarno, Rios, Anne C., and Bos, Frank L.
- Subjects
KIDNEY development ,PROGENITOR cells ,KIDNEY tubules - Abstract
Tracking and Tracing: Lgr6 marks nephron progenitor cells during kidney development. From: LGR6 marks nephron progenitor cells; Ravian L. van Ineveld, Thanasis Margaritis, Berend A.P. Kooiman, et al.; https://doi.org/10.1002/dvdy.346; DevDyn 250:11; pp. 1568–1583. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
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