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Predation structures food webs, influences energy flow, and alters rates and pathways of nutrient cycling through ecosystems, effects that are well documented for macroscopic predators. In the microbial world, predatory bacteria are common, yet little is known about their rates of growth and roles in energy flows through microbial food webs, in part because these are difficult to quantify. Here, we show that growth and carbon uptake were higher in predatory bacteria compared to nonpredatory bacteria, a finding across 15 sites, synthesizing 82 experiments and over 100,000 taxon-specific measurements of element flow into newly synthesized bacterial DNA. Obligate predatory bacteria grew 36% faster and assimilated carbon at rates 211% higher than nonpredatory bacteria. These differences were less pronounced for facultative predators (6% higher growth rates, 17% higher carbon assimilation rates), though high growth and carbon assimilation rates were observed for some facultative predators, such as members of the genera Lysobacter and Cytophaga, both capable of gliding motility and wolf-pack hunting behavior. Added carbon substrates disproportionately stimulated growth of obligate predators, with responses 63% higher than those of nonpredators for the Bdellovibrionales and 81% higher for the Vampirovibrionales, whereas responses of facultative predators to substrate addition were no different from those of nonpredators. This finding supports the ecological theory that higher productivity increases predator control of lower trophic levels. These findings also indicate that the functional significance of bacterial predators increases with energy flow and that predatory bacteria influence element flow through microbial food webs.IMPORTANCE The word "predator" may conjure images of leopards killing and eating impala on the African savannah or of great white sharks attacking elephant seals off the coast of California. But microorganisms are also predators, including bacteria that kill and eat other bacteria. While predatory bacteria have been found in many environments, it has been challenging to document their importance in nature. This study quantified the growth of predatory and nonpredatory bacteria in soils (and one stream) by tracking isotopically labeled substrates into newly synthesized DNA. Predatory bacteria were more active than nonpredators, and obligate predators, such as Bdellovibrionales and Vampirovibrionales, increased in growth rate in response to added substrates at the base of the food chain, strong evidence of trophic control. This work provides quantitative measures of predator activity and suggests that predatory bacteria-along with protists, nematodes, and phages-are active and important in microbial food webs.

Nitrous oxide (N2O) is one of the most important climate-forcing gases, and a large portion of global anthropogenic N2O emissions come from agricultural soils. Yet, how contrasting global change factors and agricultural management can interact to drive N2O emissions remains poorly understood. Here, conducted within a rice–wheat cropping system, we combined a two-year field experiment with two pot experiments to investigate the influences of elevated atmospheric carbon dioxide (eCO2) and crop straw addition to soil in altering N2O emissions under wheat cropping. Our analyses identified consistent and significant interactions between eCO2 and straw addition, whereby eCO2 increased N2O emissions (+ 19.9%) only when straw was added, and independent of different N fertilizer gradients and wheat varieties. Compared with the control (i.e., ambient CO2 without straw addition), eCO2 + straw addition increased N2O emission by 44.7% and dissolved organic carbon to total dissolved nitrogen (DOC/TDN) ratio by 115.3%. Similarly, eCO2 and straw addition significantly impacted soil N2O-related microbial activity. For instance, the ratio of the abundance of N2O production genes (i.e., nirK and nirS) to the abundance of the N2O reduction gene (i.e., nosZ) with straw addition was 26.0% higher than that without straw under eCO2. This indicates an increased denitrification potential and suggests a change in the stoichiometry of denitrification products, affecting the balance between N2O production and reduction, leading to an increase in N2O emissions. Taken together, our results emphasize the critical role of the interaction between the specific agronomic practice of straw addition and eCO2 in shaping greenhouse gas emissions in the wheat production system studied, and underline the need to test the efficacy of greenhouse gas mitigation measures under various management practices and global change scenarios. [ABSTRACT FROM AUTHOR]

The majority of the Earth's terrestrial carbon is stored in the soil. If anthropogenic warming stimulates the loss of this carbon to the atmosphere, it could drive further planetary warming. Despite evidence that warming enhances carbon fluxes to and from the soil, the net global balance between these responses remains uncertain. Here we present a comprehensive analysis of warming-induced changes in soil carbon stocks by assembling data from 49 field experiments located across North America, Europe and Asia. We find that the effects of warming are contingent on the size of the initial soil carbon stock, with considerable losses occurring in high-latitude areas. By extrapolating this empirical relationship to the global scale, we provide estimates of soil carbon sensitivity to warming that may help to constrain Earth system model projections. Our empirical relationship suggests that global soil carbon stocks in the upper soil horizons will fall by 30 ± 30 petagrams of carbon to 203 ± 161 petagrams of carbon under one degree of warming, depending on the rate at which the effects of warming are realized. Under the conservative assumption that the response of soil carbon to warming occurs within a year, a business-as-usual climate scenario would drive the loss of 55 ± 50 petagrams of carbon from the upper soil horizons by 2050. This value is around 12-17 per cent of the expected anthropogenic emissions over this period. Despite the considerable uncertainty in our estimates, the direction of the global soil carbon response is consistent across all scenarios. This provides strong empirical support for the idea that rising temperatures will stimulate the net loss of soil carbon to the atmosphere, driving a positive land carbon-climate feedback that could accelerate climate change.

Soil carbon (C) is a critical component of Earth system models (ESMs), and its diverse representations are a major source of the large spread across models in the terrestrial C sink from the third to fifth assessment reports of the Intergovernmental Panel on Climate Change (IPCC). Improving soil C projections is of a high priority for Earth system modeling in the future IPCC and other assessments. To achieve this goal, we suggest that (1) model structures should reflect real-world processes, (2) parameters should be calibrated to match model outputs with observations, and (3) external forcing variables should accurately prescribe the environmental conditions that soils experience. First, most soil C cycle models simulate C input from litter production and C release through decomposition. The latter process has traditionally been represented by first-order decay functions, regulated primarily by temperature, moisture, litter quality, and soil texture. While this formulation well captures macroscopic soil organic C (SOC) dynamics, better understanding is needed of their underlying mechanisms as related to microbial processes, depth-dependent environmental controls, and other processes that strongly affect soil C dynamics. Second, incomplete use of observations in model parameterization is a major cause of bias in soil C projections from ESMs. Optimal parameter calibration with both pool- and flux-based data sets through data assimilation is among the highest priorities for near-term research to reduce biases among ESMs. Third, external variables are represented inconsistently among ESMs, leading to differences in modeled soil C dynamics. We recommend the implementation of traceability analyses to identify how external variables and model parameterizations influence SOC dynamics in different ESMs. Overall, projections of the terrestrial C sink can be substantially improved when reliable data sets are available to select the most representative model structure, constrain parameters, and prescribe forcing fields.

Conservation agriculture (CA) has been promoted as a method of sustainable intensification and climate change mitigation and is being widely practiced and implemented globally. However, no-till (NT), a fundamental component of CA, has been shown to reduce yields in many cases. In order to maintain yields following adoption of CA, it has been recently suggested that fertilizer application should be an integral component of CA. To determine the contribution of nitrogen (N) fertilizer in minimizing yield declines following NT implementation, we assessed 2759 paired comparisons of NT and conventional tillage (CT) systems from 325 studies reported in the peer-reviewed literature between 1980 and 2013. Overall, we found that NT yields decreased −10.7% (−14.8% to −6.5%) and −3.7% (−5.3% to −2.2%) relative to CT in tropical/subtropical and temperate regions, respectively. Among management and environmental variables that included: the rate of N fertilization; the duration of the NT/CT comparison; residue, rotation, and irrigation practices; the crop type; and the site aridity, N rate was the most important explanatory variable for NT yield declines in tropical/subtropical regions. In temperate regions, N fertilization rates were relatively less important. NT yield declines were most consistently observed at low rates of N fertilization during the first 2 years of NT adoption in tropical/subtropical regions. Applications of N fertilizer at rates of up to 85±12kgNha−1yr−1 significantly reduced NT yield declines in these scenarios. While this result should not be viewed as a rate recommendation, it does suggest that farmers applying rates of N fertilizer that are low for their specific system will, on average, see higher NT yields if they increase application rates. In addition, when crop rotation was not practiced or residues were removed from the field, NT yield declines were magnified by low rates of N fertilization in tropical/subtropical regions. These results, based on a global data set and across a broad range of crops, highlight the importance of N fertilization in counteracting yield declines in NT systems, particularly in tropical/subtropical regions.

No-till agriculture represents a relatively widely adopted management system that aims to reduce soil erosion, decrease input costs, and sustain long-term crop productivity. However, its impacts on crop yields are variable, and an improved understanding of the factors limiting productivity is needed to support evidence-based management decisions. We conducted a global meta-analysis to evaluate the influence of various crop and environmental variables on no-till relative to conventional tillage yields using data obtained from peer-reviewed publications (678 studies with 6005 paired observations, representing 50 crops and 63 countries). Side-by-side yield comparisons were restricted to studies comparing conventional tillage to no-till practices in the absence of other cropping system modifications. Crop category was the most important factor influencing the overall yield response to no-till followed by aridity index, residue management, no-till duration, and N rate. No-till yields matched conventional tillage yields for oilseed, cotton, and legume crop categories. Among cereals, the negative impacts of no-till were smallest for wheat (−2.6%) and largest for rice (−7.5%) and maize (−7.6%). No-till performed best under rainfed conditions in dry climates, with yields often being equal to or higher than conventional tillage practices. Yields in the first 1–2 years following no-till implementation declined for all crops except oilseeds and cotton, but matched conventional tillage yields after 3–10 years except for maize and wheat in humid climates. Overall, no-till yields were reduced by 12% without N fertilizer addition and 4% with inorganic N addition. Our study highlights factors contributing to and/or decreasing no-till yield gaps and suggests that improved targeting and adaptation, possibly including additional system modifications, are necessary to optimize no-till performance and contribute to food production goals. In addition, our results provide a basis for conducting trade-off analyses to support the development of no-till crop management and international development strategies based on available scientific evidence.

Rising temperatures are expected to reduce global soil carbon (C) stocks, driving a positive feedback to climate change1-3. However, the mechanisms underlying this prediction are not well understood, including how temperature affects microbial enzyme kinetics, growth efficiency (MGE), and turnover4,5. Here, in a laboratory study, we show that microbial turnover accelerates with warming and, along with enzyme kinetics, determines the response of microbial respiration to temperature change. In contrast, MGE, which is generally thought to decline with warming6-8, showed no temperature sensitivity. A microbial-enzyme model suggests that such temperature sensitive microbial turnover would promote soil C accumulation with warming, in contrast to reduced soil C predicted by traditional biogeochemical models. Furthermore, the effect of increased microbial turnover differs from the effects of reduced MGE, causing larger increases in soil C stocks. Our results demonstrate that the response of soil C to warming is affected by changes in microbial turnover. This control should be included in the next generation of models to improve prediction of soil C feedbacks to warming.

The article discusses the estimation of nitrous oxide (N2O) emissions during the fallow period in cropland and the adjustment of the global-scale N2O emission factor (EF) based on the Intergovernmental Panel on Climate Change (IPCC) Tier 1 method. The authors address concerns raised by Cardinael et al. regarding the extrapolation of EF defaults, the adjusted EF for countries with dry fallow periods, and the representativeness of observations. They argue that their approach is robust and that including fallow-period precipitation (FP) does not significantly improve model performance. The authors agree on the need for more observations in under-represented regions to further improve global estimates. [Extracted from the article]

Croplands account for ~ one‐third of global anthropogenic nitrous oxide (N2O) emissions. A number of recent field experiments found substantial fallow‐period N2O emissions, which have been neglected for decades. However, the global contribution of the fallow‐period emissions and the associated drivers remain unclear. Based on 360 observations across global agroecosystems, we simulated the ratio of the fallow to the whole‐year N2O emissions (Rfallow) by developing a mixed‐effect model and compiling cropping‐system‐specific input data. Our results revealed that the mean global gridded Rfallow was 44% (15%–75%, 95% confidence interval), with hotspots mainly in the northern high latitudes. For most cropping systems, soil pH was the dominant driver of global variation in Rfallow. Global cropland emission factors (i.e., the percentage of fertilizer N emitted as N2O, EFs) in EF‐based models doubled to 1.9% when the fallow‐period N2O emissions were included in our simulation, similar to EFs estimated by process‐based and atmospheric inversion models (1.8%–2.3%). Overall, our study highlights the importance of fallow‐period N2O emissions in annual totals, especially for single cropping systems and croplands in acidic areas. To accurately estimate N2O emissions for national greenhouse gas inventories, it is crucial to update current EFs with full consideration of the fallow‐period N2O emissions in the Intergovernmental Panel on Climate Change (IPCC) Tier 1 method. [ABSTRACT FROM AUTHOR]

Mineral‐associated soil organic matter (MAOM) is the largest, slowest cycling pool of carbon (C) in the terrestrial biosphere. MAOM is primarily derived from plant and microbial sources, yet the relative contributions of these two sources to MAOM remain unresolved. Resolving this issue is essential for managing and modeling soil carbon responses to environmental change. Microbial biomarkers, particularly amino sugars, are the primary method used to estimate microbial versus plant contributions to MAOM, despite systematic biases associated with these estimates. There is a clear need for independent lines of evidence to help determine the relative importance of plant versus microbial contributions to MAOM. Here, we synthesized 288 datasets of C/N ratios for MAOM, particulate organic matter (POM), and microbial biomass across the soils of forests, grasslands, and croplands. Microbial biomass is the source of microbial residues that form MAOM, whereas the POM pool is the direct precursor of plant residues that form MAOM. We then used a stoichiometric approach—based on two‐pool, isotope‐mixing models—to estimate the proportional contribution of plant residue (POM) versus microbial sources to the MAOM pool. Depending on the assumptions underlying our approach, microbial inputs accounted for between 34% and 47% of the MAOM pool, whereas plant residues contributed 53%–66%. Our results therefore challenge the existing hypothesis that microbial contributions are the dominant constituents of MAOM. We conclude that biogeochemical theory and models should account for multiple pathways of MAOM formation, and that multiple independent lines of evidence are required to resolve where and when plant versus microbial contributions are dominant in MAOM formation. [ABSTRACT FROM AUTHOR]

Anthropogenic nitrogen (N) loading alters soil ammonia‐oxidizing archaea (AOA) and bacteria (AOB) abundances, likely leading to substantial changes in soil nitrification. However, the factors and mechanisms determining the responses of soil AOA:AOB and nitrification to N loading are still unclear, making it difficult to predict future changes in soil nitrification. Herein, we synthesize 68 field studies around the world to evaluate the impacts of N loading on soil ammonia oxidizers and nitrification. Across a wide range of biotic and abiotic factors, climate is the most important driver of the responses of AOA:AOB to N loading. Climate does not directly affect the N‐stimulation of nitrification, but does so via climate‐related shifts in AOA:AOB. Specifically, climate modulates the responses of AOA:AOB to N loading by affecting soil pH, N‐availability and moisture. AOB play a dominant role in affecting nitrification in dry climates, while the impacts from AOA can exceed AOB in humid climates. Together, these results suggest that climate‐related shifts in soil ammonia‐oxidizing community maintain the N‐stimulation of nitrification, highlighting the importance of microbial community composition in mediating the responses of the soil N cycle to N loading. [ABSTRACT FROM AUTHOR]

The article "Climate change mitigation through soil carbon sequestration in working lands: A reality check" questions the validity and realism of claims made by Almaraz et al. regarding the potential magnitude of soil carbon sequestration (SCS) in agricultural soils. The authors argue that Almaraz et al. overestimate the availability of organic matter for compost and biochar, inaccurately interpret carbon sequestration rates, and make premature assumptions about the applicability of enhanced rock weathering. They emphasize the need for realistic evaluations of the limitations and trade-offs of SCS practices and caution against overly optimistic estimates that can mislead policymakers. The authors declare no conflicts of interest. [Extracted from the article]

A global meta-analysis of conservation agriculture principles indicates that the potential contribution of no-till to the sustainable intensification of agriculture is more limited than often assumed. Does conservation agriculture work? Advocates of conservation agriculture, which integrates ecological management (namely zero tillage, permanent soil cover and crop rotation) with modern agricultural production techniques, see it as a means of sustainably increasing global food supply. So far it has proved difficult to establish conclusively whether crop yields are maintained by conservation agriculture and whether it can be applied effectively in widely differing farming contexts. In this meta-analysis of more than 5,000 observations from 610 studies, Cameron Pittelkow and colleagues show that farming using a combination of conservation agriculture techniques can yield as much or more than conventional farming under certain conditions. The use of 'no-till' alone, the central concept of conservation agriculture, negatively impacts yields. But used with the other two conservation agriculture techniques of residue retention and crop rotation, no-till can increase rainfed crop productivity in dry climates, suggesting it may become an important climate-change adaptation strategy. One of the primary challenges of our time is to feed a growing and more demanding world population with reduced external inputs and minimal environmental impacts, all under more variable and extreme climate conditions in the future.sup.1,2,3,4. Conservation agriculture represents a set of three crop management principles that has received strong international support to help address this challenge.sup.5,6, with recent conservation agriculture efforts focusing on smallholder farming systems in sub-Saharan Africa and South Asia.sup.7. However, conservation agriculture is highly debated, with respect to both its effects on crop yields.sup.8,9,10 and its applicability in different farming contexts.sup.7,11,12,13. Here we conduct a global meta-analysis using 5,463 paired yield observations from 610 studies to compare no-till, the original and central concept of conservation agriculture, with conventional tillage practices across 48 crops and 63 countries. Overall, our results show that no-till reduces yields, yet this response is variable and under certain conditions no-till can produce equivalent or greater yields than conventional tillage. Importantly, when no-till is combined with the other two conservation agriculture principles of residue retention and crop rotation, its negative impacts are minimized. Moreover, no-till in combination with the other two principles significantly increases rainfed crop productivity in dry climates, suggesting that it may become an important climate-change adaptation strategy for ever-drier regions of the world. However, any expansion of conservation agriculture should be done with caution in these areas, as implementation of the other two principles is often challenging in resource-poor and vulnerable smallholder farming systems, thereby increasing the likelihood of yield losses rather than gains. Although farming systems are multifunctional, and environmental and socio-economic factors need to be considered.sup.14,15,16, our analysis indicates that the potential contribution of no-till to the sustainable intensification of agriculture is more limited than often assumed., Author(s): Cameron M. Pittelkow [sup.1] [sup.8] , Xinqiang Liang [sup.2] , Bruce A. Linquist [sup.1] , Kees Jan van Groenigen [sup.3] , Juhwan Lee [sup.4] , Mark E. Lundy [sup.1] [...]