The continual increase in domestic and industrial wastewater poses a significant challenge, as conventional wastewater treatment methods struggle to effectively remove persistent organic micropollutants (OMPs). OMPs, including pharmaceuticals, personal care products (PPCPs), and pesticides, persist in treated water, posing risks to human health and ecosystems. Current water quality in Europe is compromised by the prevalence of OMPs. While various technologies exist for OMP removal, they often entail high costs and carbon footprints. This study explores the potential of bioremediation, particularly using wood and litter-degrading fungi (WLDF), such as Agaricus bisporus, as a sustainable and cost-effective solution. A. bisporus, commonly known as the champignon mushroom, is a prolific WLDF. The mushroom's substrate, a waste product after harvest, is rich in lignin-modifying enzymes (LMEs) that have demonstrated OMP removal capabilities. LMEs, including lignin peroxidase (LiP), manganese peroxidase (MnP), versatile peroxidase (VP), and laccase (Lcc), are essential in mineralizing lignin. This study investigates whether A. bisporus spent mushroom substrate (SMS) can be utilized for OMP removal from water and aims to elucidate the underlying mechanisms. Research indicates that A. bisporus SMS and its tea exhibit substantial OMP removal capabilities. They effectively remove a variety of dyes, PPCPs, pesticides, and even industrial compounds like per- and polyfluoroalkyl substances (PFAS). This study reports, for the first time, the removal of PFAS using SMS and its tea. Furthermore, other WLDF, such as Pleurotus ostreatus and Trametes versicolor, also show potential for OMP removal. Mechanisms of OMP removal by A. bisporus involve both enzymatic and non-enzymatic processes. LMEs play a crucial role, but non-enzymatic activities, such as the Fenton reaction, also contribute to OMP removal. The study suggests that a combination of these mechanisms is responsible for the obs