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12 results on '"van Bilsen, Kiki"'

1. Long-Term Follow-Up of Patients with Scleritis After Rituximab Treatment Including B Cell Monitoring.

Author

van Bilsen, Kiki, Vergouwen, Daphne P. C., van Velthoven, Mirjam E. J., Missotten, Tom O. A. R., Rombach, Saskia M., van Zelm, Menno C., Berkowska, Magdalena A., van Hagen, P. Martin, Kuijpers, Robert W. A. M., and van Laar, Jan A. M.

Subjects
*B cells, *SCLERITIS, *RITUXIMAB, *REFRACTORY materials
Abstract

Purpose: We report the long-term effect of rituximab (RTX) in scleritis and determine the value of B-cell monitoring for the prediction of relapses. Methods: We retrospectively studied 10 patients with scleritis, who were treated with RTX. Clinical characteristics were collected, and blood B-cell counts were measured before the start of RTX, and at various time points after treatment. Results: Clinical activity of scleritis decreased after RTX treatment in all patients within a median time of 8 weeks (range 3–13), and all reached remission. The median follow-up was 101 months (range 9–138). Relapses occurred in 6 out of 10 patients. All relapses, where B-cell counts were measured (11 out of 19), were heralded by returning B cells. However, B cells also returned in patients with long-term remissions. Conclusions: RTX is a promising therapeutic option for scleritis. Recurrence of B cells after initial depletion does not always predict relapse of scleritis. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Long-Term Follow-Up of Patients with Scleritis After Rituximab Treatment Including B Cell Monitoring

Author

van Bilsen, Kiki, primary, Vergouwen, Daphne P. C., additional, van Velthoven, Mirjam E. J., additional, Missotten, Tom O. A. R., additional, Rombach, Saskia M., additional, van Zelm, Menno C., additional, Berkowska, Magdalena A., additional, van Hagen, P. Martin, additional, Kuijpers, Robert W. A. M., additional, and van Laar, Jan A. M., additional

Published
2023
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3. Long-Term Follow-Up of Patients with Scleritis After Rituximab Treatment Including B Cell Monitoring

Author

van Bilsen, Kiki, Vergouwen, Daphne P.C., van Velthoven, Mirjam E.J., Missotten, Tom O.A.R., Rombach, Saskia M., van Zelm, Menno C., Berkowska, Magdalena A., van Hagen, P. Martin, Kuijpers, Robert W.A.M., van Laar, Jan A.M., van Bilsen, Kiki, Vergouwen, Daphne P.C., van Velthoven, Mirjam E.J., Missotten, Tom O.A.R., Rombach, Saskia M., van Zelm, Menno C., Berkowska, Magdalena A., van Hagen, P. Martin, Kuijpers, Robert W.A.M., and van Laar, Jan A.M.

Abstract

Purpose: We report the long-term effect of rituximab (RTX) in scleritis and determine the value of B-cell monitoring for the prediction of relapses. Methods: We retrospectively studied 10 patients with scleritis, who were treated with RTX. Clinical characteristics were collected, and blood B-cell counts were measured before the start of RTX, and at various time points after treatment. Results:Clinical activity of scleritis decreased after RTX treatment in all patients within a median time of 8 weeks (range 3–13), and all reached remission. The median follow-up was 101 months (range 9–138). Relapses occurred in 6 out of 10 patients. All relapses, where B-cell counts were measured (11 out of 19), were heralded by returning B cells. However, B cells also returned in patients with long-term remissions.Conclusions: RTX is a promising therapeutic option for scleritis. Recurrence of B cells after initial depletion does not always predict relapse of scleritis.

Published
2023

9. Chloroquine and Hydroxychloroquine Increase Retinal Pigment Epithelial Layer Permeability

Author

Korthagen, Nicoline, Bastiaans, Jeroen, van Meurs, Johan, van Bilsen, Kiki, van Hagen, P.M., Dik, Wim, Immunology, Ophthalmology, and Internal Medicine

Abstract

Antimalarials chloroquine (CQ) and hydroxychloroquine (HCQ) are widely used as antiinflammatory drugs, but side effects include retinopathy and vision loss. The objective of this study was to examine the effect of CQ and HCQ on the barrier integrity of retinal pigment epithelial (RPE) cell monolayers in vitro. Permeability of ARPE-19 cell monolayers was determined using Fluorescein isothiocyanate (FITC)-labeled dextran. The influence of CQ and HCQ on cell death and the expression tight junction molecules was examined. CQ and HCQ significantly increased ARPE-19 monolayer permeability after 3 and 18 h, respectively, and enhanced mRNA levels for claudin-1 and occludin. Cytotoxicity was only observed after 18 h exposure. Thus, CQ and HCQ rapidly enhance RPE barrier permeability in vitro, independent of cytotoxicity or loss of zonula occludens-1, claudin-1, and occludin expression. Our findings suggest that CQ/HCQ-induced permeability of the RPE layer may contribute to blood-retinal barrier breakdown in case of CQ/HCQ-induced retinopathy.

Published
2015

12. Chloroquine and Hydroxychloroquine Increase Retinal Pigment Epithelial Layer Permeability.

Author

Korthagen NM, Bastiaans J, van Meurs JC, van Bilsen K, van Hagen PM, and Dik WA

Subjects
Blood-Retinal Barrier metabolism, Claudin-1 drug effects, Claudin-1 genetics, Gene Expression Regulation, Humans, Occludin drug effects, Occludin genetics, Permeability drug effects, RNA, Messenger, Retinal Pigment Epithelium metabolism, Tight Junctions metabolism, Antimalarials pharmacology, Blood-Retinal Barrier drug effects, Chloroquine pharmacology, Hydroxychloroquine pharmacology, Retinal Pigment Epithelium drug effects, Tight Junctions drug effects
Abstract

Antimalarials chloroquine (CQ) and hydroxychloroquine (HCQ) are widely used as antiinflammatory drugs, but side effects include retinopathy and vision loss. The objective of this study was to examine the effect of CQ and HCQ on the barrier integrity of retinal pigment epithelial (RPE) cell monolayers in vitro. Permeability of ARPE-19 cell monolayers was determined using Fluorescein isothiocyanate (FITC)-labeled dextran. The influence of CQ and HCQ on cell death and the expression tight junction molecules was examined. CQ and HCQ significantly increased ARPE-19 monolayer permeability after 3 and 18 h, respectively, and enhanced mRNA levels for claudin-1 and occludin. Cytotoxicity was only observed after 18 h exposure. Thus, CQ and HCQ rapidly enhance RPE barrier permeability in vitro, independent of cytotoxicity or loss of zonula occludens-1, claudin-1, and occludin expression. Our findings suggest that CQ/HCQ-induced permeability of the RPE layer may contribute to blood-retinal barrier breakdown in case of CQ/HCQ-induced retinopathy., (© 2015 Wiley Periodicals, Inc.)

Published
2015
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