1. Metabolomic screening and urolithins metabotypes identification in the urinary metabolome of Costa Rican volunteers after blackberry (<italic>Rubus adenotrichos</italic>)-based drink consumption.
- Author
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Fallas-Ramirez, José-Manuel, Baltodano-Viales, Eleaneth, Mora-Román, Juan-José, Hernandez, Lorena, Pérez, Ana M., and Vaillant, Fabrice
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FUNCTIONAL foods , *NUTRITIONAL value , *LIQUID chromatography , *LIQUID analysis , *VOLUNTEERS - Abstract
Besides providing nutritional value, functional foods have potential beneficial effects on health. Consumers worldwide seek food products with added value based on physical and mental well-being.This study aimed to analyze the urinary metabolome of volunteers who consumed a drink based on Costa Rican blackberries (
Rubus adenotrichos ) to identify the presence of urolithins and other possible bioactive compounds related to beneficial effects on health.Fifteen healthy non-smoking male volunteers aged between 19 and 43 years old who consumed 250 ml of blackberry-based drink for breakfast for eight consecutive days were selected. Urine samples were obtained before and after drink consumption, which were submitted to analysis by Ultra-Performance Liquid Chromatography coupled to a Mass Detector (UPLC/ESI-Q-TOF-MS). Subsequently, a metabolomic analysis of the results was performed to identify the presence or absence of urolithins and other bioactive metabolites related to blackberry juice consumption.It was possible to detect high intensity presence of urolithin A (n = 6) and high intensity presence of both urolithin A and B (n = 5) in urine samples in several volunteers. However, finding them was impossible in others, or they were at very low intensities (n = 4). Likewise, it was possible to identify Menth-1-en-4,8 diol glucuronide and 1-O-E-Cinnamoyl-(6-arabinosylglucose) as other compounds related to the consumption of blackberries.The consumption of Costa Rican blackberries allowed us to demonstrate the presence of three metabotypes within the study population: 40% of strong urolithin A (UAP) producers, 33.3% of both urolithin (A and B) producers (UBP), and 26.7% of weak urolithin A producers (0P). Finally, it is recommended to carry out more research in this regard, considering a greater number of volunteers and including people of both sexes. [ABSTRACT FROM AUTHOR]- Published
- 2024
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