1. Molecular Surveillance of Antimicrobial Resistance of Mycobacterium leprae from Leprosy Patients in Zhejiang Province, China
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Shi Y, Kong W, Jiang H, Zhang W, Wang C, Wu L, Shen Y, Yao Q, and Wang H
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purpose: reports on antimicrobial resistance (amr) of mycobacterium leprae (m. leprae) in zhejiang province are limited. thus ,this study aimed to investigate the drug resistance of new leprosy cases within several years and analyse the emergence of amr mutations from zhejiang province. methods: this study enrolled 34 leprosy cases in zhejiang province ,china ,from 2018 to 2021. gene mutation of who-recommended drdrs (folp1 ,rpob and gyra) and genes of compensatory amr-associated drdrs ,including nth ,rpoa ,rpoc ,gyrb and 23s rrna ,were detected by amplification. clinical data analysis was performed to investigate the epidemiological association of leprosy. results: of the 34 samples ,two (5.9%) strains showed drug resistance ,which were mutated to dapsone and ofloxacin ,separately. two single mutations in gyrb were detected in different strains (5.9%) ,whereas one of the rpoc mutation was also detected in one strain each (2.9%) ,which were proved to be polymorphs. no correlation of drug resistance proportion was identified in male vs. female ,nerve vs. no nerve involvement ,deformity vs. no deformity and reaction vs. non-reaction cases. conclusion: results showed well control of leprosy patients in zhejiang province. gene mutations of who-recommended drdrs folp1 and gyra confirmed the resistance to dapsone and ofloxacin. compensatory amr-associated mutations confirmed to be polymorphs still require further study to determine their phenotypic outcomes in m. leprae. the results demonstrated that drug-resistant strains are not epidemic in this area. given the few cases of leprosy ,analysing the amr of m. leprae in zhejiang province more comprehensively is difficult. however ,regular mdt treatment and population management in the early stage may contribute to the low prevalence of leprosy. ,Infectious and parasitic diseases ,RC109-216 - Abstract
Ying Shi1– 3*, Wenming Kong1 *, Haiqin Jiang,2,3 Wenyue Zhang,2,3 Chen Wang,2– 4 Limei Wu,1 Yunliang Shen,1 Qiang Yao,1 Hongsheng Wang1– 3,5 1Department of Leprosy Control, Zhejiang Provincial Institute of Dermatology, Huzhou, People’s Republic of China; 2Hospital of Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, Jiangsu Key Laboratory of Molecular Biology for Skin Diseases and STIs, Nanjing, People’s Republic of China; 3National Centre for STD and Leprosy Control, China CDC, Nanjing, People’s Republic of China; 4Department of Epidemiology and Biostatistics, School of Public Health, Nanjing Medical University, Nanjing, People’s Republic of China; 5Centre for Global health, School of Public Health, Nanjing Medical University, Nanjing, People’s Republic of China* These authors contributed equally to this workCorrespondence: Qiang Yao, Department of Leprosy Control, Zhejiang Provincial Institute of Dermatology, St 61, Zhejiang, People’s Republic of China, Email 7793908@qq.com Hongsheng Wang, Institute of Dermatology, Chinese Academy of Medical Sciences & Peking Union Medical College, No. 12 Jiangwangmiao Street, Nanjing, People’s Republic of China, Tel: +86 025 8547 8953, Email whs33@vip.sina.comPurpose: Reports on antimicrobial resistance (AMR) of Mycobacterium leprae (M. leprae) in Zhejiang Province are limited. Thus, this study aimed to investigate the drug resistance of new leprosy cases within several years and analyse the emergence of AMR mutations from Zhejiang Province.Methods: This study enrolled 34 leprosy cases in Zhejiang Province, China, from 2018 to 2021. Gene mutation of WHO-recommended DRDRs (folP1, rpoB and gyrA) and genes of compensatory AMR-associated DRDRs, including nth, rpoA, rpoC, gyrB and 23S rRNA, were detected by amplification. Clinical data analysis was performed to investigate the epidemiological association of leprosy.Results: Of the 34 samples, 2 (5.9%) strains showed drug resistance, which were mutated to dapsone and ofloxacin, separately. Two single mutations in gyrB were detected in different strains (5.9%), whereas one of the rpoC mutation was also detected in one strain each (2.9%), which were proved to be polymorphs. No correlation of drug resistance proportion was identified in male vs female, nerve vs no nerve involvement, deformity vs no deformity and reaction vs non-reaction cases.Conclusion: Results showed well control of leprosy patients in Zhejiang Province. Gene mutations of WHO-recommended DRDRs folP1 and gyrA confirmed the resistance to dapsone and ofloxacin. Compensatory AMR-associated mutations confirmed to be polymorphs still require further study to determine their phenotypic outcomes in M. leprae. The results demonstrated that drug-resistant strains are not epidemic in this area. Given the few cases of leprosy, analysing the AMR of M. leprae in Zhejiang Province more comprehensively is difficult. However, regular MDT treatment and population management in the early stage may contribute to the low prevalence of leprosy.Keywords: M. leprae, antimicrobial resistance, gene mutation
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- 2022