1. A genome-scale analysis of mRNAs targeting to plant mitochondria: upstream AUGs in 5’untranslated regions reduce mitochondrial association
- Author
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Elodie Ubrig, Audrey Vingadassalon, Laurence Maréchal-Drouard, Thalia Salinas, Timothée Vincent, Anne-Marie Duchêne, Stéfanie Graindorge, Ola Srour, Kevin Azeredo, Valérie Cognat, Institut de biologie moléculaire des plantes (IBMP), and Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,Untranslated region ,Plant Science ,Mitochondrion ,Biology ,Ribosome ,uAUG ,Mitochondrial Proteins ,03 medical and health sciences ,Cytosol ,Gene expression ,Genetics ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,RNA, Messenger ,3' Untranslated Regions ,tRNA ,ComputingMilieux_MISCELLANEOUS ,Solanum tuberosum ,translation tRNA ,Cell Nucleus ,Three prime untranslated region ,3' utr ,Translation (biology) ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,Cell Biology ,Protein subcellular localization prediction ,Mitochondria ,Cell biology ,Protein Transport ,030104 developmental biology ,ribosome ,RNA, Plant ,Mutation ,Transfer RNA ,mRNA localization ,5' Untranslated Regions ,Ribosomes ,respiration ,uORF - Abstract
Summary Intracellular sorting of mRNAs is an essential process for regulating gene expression and protein localization. Most mitochondrial proteins are nuclear-encoded and imported into the mitochondria through post-translational or co-translational processes. In the latter case, mRNAs are found to be enriched in the vicinity of mitochondria. A genome-scale analysis of mRNAs associated with mitochondria has been performed to determine plant cytosolic mRNAs targeted to the mitochondrial surface. Many messengers encoding mitochondrial proteins were found associated with mitochondria. These mRNAs correspond to particular functions and complexes, such as respiration or mitoribosomes, which indicates a coordinated control of mRNA localization within metabolic pathways. In addition, upstream AUGs in 5' untranslated regions (UTRs), which modulate the translation efficiency of downstream sequences, were found to negatively affect the association of mRNAs with mitochondria. A mutational approach coupled with in vivo mRNA visualization confirmed this observation. Moreover, this technique allowed the identification of 3'-UTRs as another essential element for mRNA localization at the mitochondrial surface. Therefore, this work offers new insights into the mechanism, function and regulation of the association of cytosolic mRNAs with plant mitochondria.
- Published
- 2017
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