1. A newly developed consortium with a highly efficient thiocyanate degradation capacity: A comprehensive investigation of the degradation and detoxification potential.
- Author
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Xiao, Xiaoshuang, An, Xuejiao, Jiang, Yuling, Wang, Liuwei, Li, Zelin, Lai, Fenju, and Zhang, Qinghua
- Subjects
MUNG bean ,CELL nuclei ,GERMINATION ,RICE seeds ,REACTIVE oxygen species ,SEWAGE ,TOXICITY testing ,NUCLEOTIDE sequencing - Abstract
Thiocyanate-containing wastewater harms ecosystems and can cause serious damage to animals and plants, so it is urgent to treat it effectively. In this study, a new efficient thiocyanate-degrading consortium was developed and its degradation characteristics were studied. It was found that up to 154.64 mM thiocyanate could be completely degraded by this consortium over 6 days of incubation, with a maximum degradation rate of 1.53 mM h
−1 . High-throughput sequencing analysis showed that Thiobacillus (77.78%) was the predominant thiocyanate-degrading bacterial genus. Plant toxicology tests showed that the germination index of mung bean and rice seeds cultured with media obtained after thiocyanate degradation by the consortium increased by 94% and 84.83%, respectively, compared with the control group without thiocyanate degradation. Cytotoxicity tests showed that thiocyanate without degradation significantly decreased the Neuro-2a cell activity and mitochondrial membrane potential; induced reactive oxygen species generation and apoptosis; increased the cellular Ca2+ concentration; and damaged the cell nucleus and DNA. Furthermore, the thiocyanate degradation products produced the consortium were almost totally non-toxic, revealing the same characteristics as those of the control using distilled water. This study shows that the consortium has a high degradation efficiency and detoxification characteristics, as well as great application potential in bioremediation of industrial thiocyanate-containing wastewater. [Display omitted] • A new consortium with an efficient thiocyanate-degrading ability was enriched. • The consortium can degrade up to 154.64 mM thiocyanate with a V max of 1.53 mM h−1 • The toxic thiocyanate action mechanism was revealed by cytotoxicity tests. • Thiocyanate disrupts the homeostasis of cellular physiological indicators. • Thiocyanate degradation by the consortium could eliminate its toxicity. [ABSTRACT FROM AUTHOR]- Published
- 2023
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