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2 results on '"therapeutic venom extract"'

1. Allergen Content of Therapeutic Preparations for Allergen-Specific Immunotherapy of European Paper Wasp Venom Allergy.

Author

Grosch, Johannes, Lesur, Antoine, Kler, Stéphanie, Bernardin, François, Dittmar, Gunnar, Francescato, Elisabetta, Hewings, Simon J., Jakwerth, Constanze A., Zissler, Ulrich M., Heath, Matthew D., Ollert, Markus, Kramer, Matthias F., Hilger, Christiane, Bilò, Maria Beatrice, Schmidt-Weber, Carsten B., and Blank, Simon

Subjects
*ALLERGENS, *SNAKE venom, *VENOM, *ALLERGENIC extracts, *WASPS, *IMMUNOTHERAPY, *LIQUID chromatography-mass spectrometry, *VENOM hypersensitivity
Abstract

Allergy to Polistes dominula (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have an impact on diagnostic and therapeutic approaches, the aim was to compare five therapeutic preparations for VIT of P. dominula venom allergy available in Spain. Products from five different suppliers were analyzed by SDS-PAGE and LC-MS/MS and compared with a reference venom sample. Three products with P. dominula venom and one product with a venom mixture of American Polistes species showed a comparable band pattern in SDS-PAGE as the reference sample and the bands of the major allergens phospholipase A1 and antigen 5 were assignable. The other product, which consists of a mixture of American Polistes species, exhibited the typical band pattern in one, but not in another sample from a second batch. All annotated P. dominula allergens were detected at comparable levels in LC-MS/MS analysis of products containing P. dominula venom. Due to a lack of genomic information on the American Polistes species, the remaining products were not analyzed by this method. The major Polistes allergens were present in comparable amounts in the majority, but not in all investigated samples of venom preparations for VIT of P. dominula venom allergy. [ABSTRACT FROM AUTHOR]

Published
2022
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2. Allergen content of therapeutic preparations for allergen-specific immunotherapy of European paper wasp venom allergy

Author

Johannes Grosch, Antoine Lesur, Stéphanie Kler, François Bernardin, Gunnar Dittmar, Elisabetta Francescato, Simon J. Hewings, Constanze A. Jakwerth, Ulrich M. Zissler, Matthew D. Heath, Markus Ollert, Matthias F. Kramer, Christiane Hilger, Maria Beatrice Bilò, Carsten B. Schmidt-Weber, and Simon Blank

Subjects
allergen-specific immunotherapy, American Polistes species, European paper wasp, European Polistes species, Hymenoptera venom allergy, Polistes dominula, therapeutic venom extract, venom immunotherapy, Health, Toxicology and Mutagenesis, Wasps, Wasp Venoms, Allergens, American Polistes Species, European Polistes Species, European Paper Wasp, Hymenoptera Venom Allergy, Polistes Dominula, Allergen-specific Immunotherapy, Therapeutic Venom Extract, Venom Immunotherapy, Toxicology, complex mixtures, ddc, Article, American, European, \n, Desensitization, Immunologic, Tandem Mass Spectrometry, Hypersensitivity, Animals, Chromatography, Liquid
Abstract

Allergy to Polistes dominula (European paper wasp) venom is of particular relevance in Southern Europe, potentially becoming a threat in other regions in the near future, and can be effectively cured by venom immunotherapy (VIT). As allergen content in extracts may vary and have an impact on diagnostic and therapeutic approaches, the aim was to compare five therapeutic preparations for VIT of P. dominula venom allergy available in Spain. Products from five different suppliers were analyzed by SDS-PAGE and LC-MS/MS and compared with a reference venom sample. Three products with P. dominula venom and one product with a venom mixture of American Polistes species showed a comparable band pattern in SDS-PAGE as the reference sample and the bands of the major allergens phospholipase A1 and antigen 5 were assignable. The other product, which consists of a mixture of American Polistes species, exhibited the typical band pattern in one, but not in another sample from a second batch. All annotated P. dominula allergens were detected at comparable levels in LC-MS/MS analysis of products containing P. dominula venom. Due to a lack of genomic information on the American Polistes species, the remaining products were not analyzed by this method. The major Polistes allergens were present in comparable amounts in the majority, but not in all investigated samples of venom preparations for VIT of P. dominula venom allergy.

Published
2022

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