6 results on '"the Replica Project Group"'
Search Results
2. Cytotoxicity, mutagenicity and genotoxicity of electronic cigarettes emission aerosols compared to cigarette smoke: the REPLICA project
- Author
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Rosalia Emma, Virginia Fuochi, Alfio Distefano, Konstantinos Partsinevelos, Sonja Rust, Fahad Zadjali, Mohammed Al Tobi, Razan Zadjali, Zaina Alharthi, Roberta Pulvirenti, Pio Maria Furneri, Riccardo Polosa, Ang Sun, Massimo Caruso, Giovanni Li Volti, and the Replica Project Group
- Subjects
Medicine ,Science - Abstract
Abstract Concerns have recently increased that the integrity of some scientific research is questionable due to the inability to reproduce the claimed results of some experiments and thereby confirm that the original researcher's conclusions were justified. This phenomenon has been described as 'reproducibility crisis' and affects various fields from medicine to basic applied sciences. In this context, the REPLICA project aims to replicate previously conducted in vitro studies on the toxicity of cigarette smoke and e-cigarette aerosol, sometimes adding experiments or conditions where necessary, in order to verify the robustness and replicability of the data. In this work the REPLICA Team replicated biological and toxicological assessment published by Rudd and colleagues in 2020. As in the original paper, we performed Neutral Red Uptake (NRU) assay for the evaluation of cytotoxicity, Ames test for the evaluation of mutagenesis and In Vitro Micronuclei (IVMN) assay for the evaluation of genotoxicity on cells treated with cigarette smoke or e-cigarette aerosol. The results showed high cytotoxicity, mutagenicity and genotoxicity induced by cigarette smoke, but slight or no cytotoxic, mutagenic and genotoxic effects induced by the e-cigarette aerosol. Although the two studies presented some methodological differences, the findings supported those previously presented by Rudd and colleagues.
- Published
- 2023
- Full Text
- View/download PDF
3. Electronic nicotine delivery systems exhibit reduced bronchial epithelial cells toxicity compared to cigarette: the Replica Project
- Author
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Massimo Caruso, Rosalia Emma, Alfio Distefano, Sonja Rust, Konstantinos Poulas, Fahad Zadjali, Antonio Giordano, Vladislav Volarevic, Konstantinos Mesiakaris, Mohammed Al Tobi, Silvia Boffo, Aleksandar Arsenijevic, Pietro Zuccarello, Cesarina Giallongo, Margherita Ferrante, Riccardo Polosa, Giovanni Li Volti, and the Replica Project Group
- Subjects
Medicine ,Science - Abstract
Abstract Electronic nicotine delivery systems (ENDS) may reduce health risks associated with chronic exposure to smoke and their potential benefits have been the matter of intense scientific debate. We aimed to replicate three published studies on cytotoxic and inflammatory effects of cigarette smoke and ENDS aerosol in an independent multi-center ring study. We aimed to establish the reliability of results and the robustness of conclusions by replicating the authors’ experimental protocols and further validating them with different techniques. Human bronchial epithelial cells (NCI-H292) were exposed to cigarette whole smoke and vapor phase and to aerosol from ENDS. We also assessed the inflammatory cytokines interleukin-6 and interleukin-8 and the remodeling mediator matrix metalloproteinase-1. We replicated cell viability results and confirmed that almost 80% of cytotoxic effects are due to volatile compounds in the vapor phase of smoke. Our findings substantiated the reduced cytotoxic effects of ENDS aerosol. However, our data on inflammatory and remodeling activity triggered by smoke differed significantly from those in the original reports. Taken together, independent data from multiple laboratories clearly demonstrated the reduced toxicity of ENDS products compared to cigarettes.
- Published
- 2021
- Full Text
- View/download PDF
4. Cytotoxicity, mutagenicity and genotoxicity of electronic cigarettes emission aerosols compared to cigarette smoke: the REPLICA project.
- Author
-
Emma, Rosalia, Fuochi, Virginia, Distefano, Alfio, Partsinevelos, Konstantinos, Rust, Sonja, Zadjali, Fahad, Al Tobi, Mohammed, Zadjali, Razan, Alharthi, Zaina, Pulvirenti, Roberta, Furneri, Pio Maria, Polosa, Riccardo, Sun, Ang, Caruso, Massimo, Li Volti, Giovanni, the Replica Project Group, Giordano, Antonio, Volarevic, Vladislav, Lesmana, Ronny, and Poulas, Konstantinos
- Subjects
ELECTRONIC cigarettes ,SMOKING ,CYTOTOXINS ,AEROSOLS ,CIGARETTE smoke ,GENETIC toxicology ,APPLIED sciences - Abstract
Concerns have recently increased that the integrity of some scientific research is questionable due to the inability to reproduce the claimed results of some experiments and thereby confirm that the original researcher's conclusions were justified. This phenomenon has been described as 'reproducibility crisis' and affects various fields from medicine to basic applied sciences. In this context, the REPLICA project aims to replicate previously conducted in vitro studies on the toxicity of cigarette smoke and e-cigarette aerosol, sometimes adding experiments or conditions where necessary, in order to verify the robustness and replicability of the data. In this work the REPLICA Team replicated biological and toxicological assessment published by Rudd and colleagues in 2020. As in the original paper, we performed Neutral Red Uptake (NRU) assay for the evaluation of cytotoxicity, Ames test for the evaluation of mutagenesis and In Vitro Micronuclei (IVMN) assay for the evaluation of genotoxicity on cells treated with cigarette smoke or e-cigarette aerosol. The results showed high cytotoxicity, mutagenicity and genotoxicity induced by cigarette smoke, but slight or no cytotoxic, mutagenic and genotoxic effects induced by the e-cigarette aerosol. Although the two studies presented some methodological differences, the findings supported those previously presented by Rudd and colleagues. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
5. Comparative assessment of electronic nicotine delivery systems aerosol and cigarette smoke on endothelial cell migration: the Replica Project
- Author
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Caruso, Massimo, Emma, Rosaliaa, Distefano, Alfio, Rust, Sonja, Poulas, Konstantinos, Giordano, Antonio, Volarevic, Vladislav, Mesiakaris, Konstantinos, Boffo, Silvia, Arsenijevic, Aleksandar, Karanasios, Georgios, Pulvirenti, Roberta, Ilic, Aleksandar, Canciello, Angelo, Zuccarello, Pietro, Ferrante, Margherita, Polosa, Riccardo, Li Volti, Giovanni, and the Replica Project Group
- Abstract
Cigarette smoking is associated with impairment of repair mechanisms necessary for vascular endothelium homeostasis. Reducing the exposure to smoke toxicants may result in the mitigation of the harmful effect on the endothelium and cardiovascular disease development. Previous investigations performed by the tobacco industries evaluated in vitro the effect of electronic cigarette (e-cig) compared to cigarette smoke demonstrating a significant reduction in endothelial cell migration inhibition following e-cig aerosol exposure. In the present study, we replicated one of these studies, evaluating the effects of cigarette smoke on endothelial cell migration compared to e-cig and heated tobacco products. We used a multi-center approach (ring-study) to verify the robustness and reliability of the results obtained in the replicated study. Consistently with the original study, we observed a substantial reduction of the effects of e-cig and tobacco heated products on endothelial cell migration compared to cigarette smoke. In conclusion, our study further confirms the importance of e-cig and tobacco heated products as a possible harm reduction strategy for cardiovascular diseases development in smokers.
- Published
- 2021
6. Electronic nicotine delivery systems exhibit reduced bronchial epithelial cells toxicity compared to cigarette: the Replica Project.
- Author
-
Caruso, Massimo, Emma, Rosalia, Distefano, Alfio, Rust, Sonja, Poulas, Konstantinos, Zadjali, Fahad, Giordano, Antonio, Volarevic, Vladislav, Mesiakaris, Konstantinos, Al Tobi, Mohammed, Boffo, Silvia, Arsenijevic, Aleksandar, Zuccarello, Pietro, Giallongo, Cesarina, Ferrante, Margherita, Polosa, Riccardo, Li Volti, Giovanni, the Replica Project Group, Pulvirenti, Roberta, and Carota, Giuseppe
- Subjects
ELECTRONIC cigarettes ,EPITHELIAL cells ,NICOTINE ,CIGARETTES ,CIGARETTE smoke ,SMOKING - Abstract
Electronic nicotine delivery systems (ENDS) may reduce health risks associated with chronic exposure to smoke and their potential benefits have been the matter of intense scientific debate. We aimed to replicate three published studies on cytotoxic and inflammatory effects of cigarette smoke and ENDS aerosol in an independent multi-center ring study. We aimed to establish the reliability of results and the robustness of conclusions by replicating the authors' experimental protocols and further validating them with different techniques. Human bronchial epithelial cells (NCI-H292) were exposed to cigarette whole smoke and vapor phase and to aerosol from ENDS. We also assessed the inflammatory cytokines interleukin-6 and interleukin-8 and the remodeling mediator matrix metalloproteinase-1. We replicated cell viability results and confirmed that almost 80% of cytotoxic effects are due to volatile compounds in the vapor phase of smoke. Our findings substantiated the reduced cytotoxic effects of ENDS aerosol. However, our data on inflammatory and remodeling activity triggered by smoke differed significantly from those in the original reports. Taken together, independent data from multiple laboratories clearly demonstrated the reduced toxicity of ENDS products compared to cigarettes. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
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