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2 results on '"temperature-responsive polymer coatings"'

1. Temperature-responsive peptide-mimetic coating based on poly(N-methacryloyl-l-leucine): Properties, protein adsorption and cell growth.

Author

Raczkowska, Joanna, Ohar, Mariya, Stetsyshyn, Yurij, Zemła, Joanna, Awsiuk, Kamil, Rysz, Jakub, Fornal, Katarzyna, Bernasik, Andrzej, Ohar, Halyna, Fedorova, Svitlana, Shtapenko, Oksana, Polovkovych, Svyatoslav, Novikov, Volodymyr, and Budkowski, Andrzej

Subjects
*LEUCINE, *PEPTIDES, *GABA agonists, *PROTEIN analysis, *ADSORPTION (Chemistry), *GROWTH factors
Abstract

Highlights: [•] Poly(N-methacryloyl-l-leucine) coatings were polymerized from peroxided substrate. [•] Temperature-responsive properties of the peptide-mimetic coating were discovered. [•] High affinity of PNML coating to two proteins: LcH and BSA was observed. [•] Excellent cytocompatibility of PNML coating was demonstrated for HEK 293 cells. [ABSTRACT FROM AUTHOR]

Published
2014
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2. Temperature-responsive peptide-mimetic coating based on poly(N-methacryloyl-l-leucine) : properties, protein adsorption and cell growth

Author

Joanna Zemła, Andrzej Bernasik, Katarzyna Fornal, Halyna Ohar, Volodymyr Novikov, O. V. Shtapenko, Joanna Raczkowska, Kamil Awsiuk, Mariya Ohar, Jakub Rysz, Andrzej Budkowski, Svyatoslav Polovkovych, Yurij Stetsyshyn, and Svitlana Fedorova

Subjects
Spectrometry, Mass, Secondary Ion, wettability, engineering.material, Microscopy, Atomic Force, Peroxide, Polymerization, chemistry.chemical_compound, Colloid and Surface Chemistry, Adsorption, Coated Materials, Biocompatible, Coating, Leucine, temperature-responsive polymer coatings, Polymer chemistry, Animals, Humans, Physical and Theoretical Chemistry, Bovine serum albumin, Fluorescein, biomimetic polymer coatings, Cell Proliferation, biology, Temperature, Water, Substrate (chemistry), Serum Albumin, Bovine, Surfaces and Interfaces, General Medicine, protein adsorption, graft polymerization, HEK293 Cells, Microscopy, Fluorescence, chemistry, Chemical engineering, Wettability, engineering, biology.protein, Cattle, Peptides, poly(N-methacryloyl amino acids), Biotechnology, Protein adsorption
Abstract

Poly( N -methacryloyl- l -leucine) (PNML) coatings were successfully fabricated via polymerization from peroxide initiator grafted to premodified glass substrate. Chemical composition and thickness of PNML coatings were determined using time of flight-secondary ion mass spectrometry (TOF- SIMS) and ellipsometry, respectively. PNML coatings exhibit thermal response of the wettability, between 4 and 28 °C, which indicates a transition between hydrated loose coils and hydrophobic collapsed chains. Morphology of the PNML coating was observed with the AFM, transforming with increasing temperature from initially relatively smooth surface to rough and more structured surface. Protein adsorption observed by fluorescence microscopy for model proteins (bovine serum albumin and lentil lectin labeled with fluorescein isothiocyanate) at transition from 5 to 25 °C, showed high affinity of PNML coating to proteins at all investigated temperatures and pH. Thus, PNML coating have significant potential for medical and biotechnological applications as protein capture agents or functional replacements of antibodies (“plastic antibodies”). The high proliferation growth of the human embryonic kidney cell (HEK 293) onto PNML coating was demonstrated, indicating its excellent cytocompatibility.

Published
2014

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