7 results on '"tb test"'
Search Results
2. Is TB Testing Associated With Increased Blood Interferon-Gamma Levels?
- Author
-
Aideen E. Kennedy, Jim O’Mahony, Noel Byrne, John MacSharry, and Riona G. Sayers
- Subjects
Johne’s disease ,TB test ,interferon-gamma ,purified protein derivative ,ELISA ,Veterinary medicine ,SF600-1100 - Abstract
The Republic of Ireland reports a relatively low prevalence of Johne’s disease (JD) compared to international counterparts. Postulated reasons for this include a lower average herd size and a grass-based production system. Ireland also engages in high levels of bovine tuberculosis (bTB) testing. As interferon-gamma (IFN-γ) is believed to play a key role in protecting against JD, it is our hypothesis that administration of purified protein derivative (PPD), as part of the bTB test, is associated with a systemic increase in IFN-γ production, which may potentially limit clinical progression of the disease. We studied 265 cows (202 Friesian and 63 “Non-Friesian,” e.g., JerseyX, Norwegian Red) to assess IFN-γ levels and Mycobacterium avium subspecies paratuberculosis (MAP) antibody response before and after the bTB test. As part of the compulsory annual bTB test, avian and bovine PPD were administered at two separate cervical sites. To assess IFN-γ production, blood samples were taken before and 72 h after PPD administration. MAP antibody response was assessed before and 10 days post-PPD administration. A significant increase in MAP antibody response was identified post-bTB compared to pre-bTB response (p
- Published
- 2017
- Full Text
- View/download PDF
3. DRUG SUSCEPTIBILITY AND GENOTYPE AFFINITY OF MYCOBACTERIUM TUBERCULOSIS ISOLATED FROM SURGICAL SPECIMENS OF PULMONARY TUBERCULOSIS PATIENTS
- Author
-
K. V. Belousovа, T. V. Umpelevа, N. I. Eremeevа, L. A. Golubevа, T. Yu. Botevа, and D. V. Vаkhrushevа
- Subjects
0301 basic medicine ,medicine.medical_specialty ,tb test ,Tuberculosis ,lung surgical specimens ,030106 microbiology ,Drug resistance ,Gastroenterology ,drug susceptibility ,Mycobacterium tuberculosis ,Diseases of the respiratory system ,03 medical and health sciences ,Internal medicine ,Genotype ,medicine ,mycobacterium tuberculosis ,RC705-779 ,biology ,business.industry ,Isoniazid ,General Medicine ,biology.organism_classification ,medicine.disease ,Multiple drug resistance ,Beijing family ,030104 developmental biology ,biochip diagnostics ,business ,bacteriological diagnostics ,Rifampicin ,medicine.drug - Abstract
The objective of the study : to investigate the spectrum of drug resistance and genotypes of Mycobacterium tuberculosis isolated from lung surgical specimens of tuberculosis patients. Subjects and methods . Bacteriological testing of surgical specimens of 287 patients operated due to tuberculosis was performed. Molecular genetic tests detected Mycobacterium tuberculosis with multiple drug resistance in 66.7% of patients. Test results of 42 samples were compared with culture results and a high level of coincidence was observed for isoniazid (90.9%), rifampicin (89.3%) and fluoroquinolones (81.2%). It was found out that the majority of samples (80.8%) belonged to Beijing family.
- Published
- 2019
4. The single intradermal cervical comparative test interferes with Johne's disease ELISA diagnostics.
- Author
-
Kennedy, Aideen E., Da Silva, Ana T., Byrne, Noel, Govender, Rodney, MacSharry, John, O'Mahony, Jim, and Sayers, Riona G.
- Subjects
PARATUBERCULOSIS ,ENZYME-linked immunosorbent assay ,MYCOBACTERIUM avium paratuberculosis ,SENSITIVITY analysis ,ENZYME specificity ,COMPARATIVE studies ,DIAGNOSIS - Abstract
Enzyme-linked immunosorbent assays (ELISA) of milk and serum samples are a routinely used method of screening herds for Mycobacterium avium subspecies paratuberculosis (MAP). Infection with MAP causes granulomatous enteritis of ruminants known as Johne's disease (JD). The sensitivity (Se) and specificity (Sp) of MAP ELISAs leads to difficulties in the identification of both infected and infectious animals. Interference with MAP ELISA Se and Sp has been reported in MAP seronegative cows following administration of purified protein derivative (PPD) as part of intradermal testing for bovine tuberculosis (bTB). The aim of this study is to examine the impact of the single intradermal cervical comparative test (SICCT) for bTB, on both serum and milk MAP ELISA tests, in a herd containing both seropositive and seronegative cows pre-SICCT. A secondary objective is to provide appropriate timing of JD ELISA tests in relation to the SICCT. A herd of 139 cowswere serum and milk sampled pre- and post-SICCT administration. Prior to SICCT, 6% of the herd tested seropositive for MAP using milk ELISA, with 8% positive on serum. ID Screen Paratuberculosis Indirect Screening Test (ID Vet) was used to screen the herd. Within 14 days of PPD administration, a significant increase in the prevalence of seropositive cows was recorded. Identical prevalence's were recorded with both test matrices (39%). ELISA values remained significantly higher until day 43 post-SICCT in milk (P D0.850), and day 71 in serum (P D0.602). If the "new" positives detected post-b TB testing are deemed false positives due to generation of cross-reacting antibodies by administration of PPD, milk would appear a more suitable sample for JD ELISA testing within 2 months of SICCT. In summary, sampling for JD utilizing milk ELISA should be avoided in the 43-day period following PPD administration, with serum ELISA sampling avoided for an additional 28 days. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
5. The Single Intradermal Cervical Comparative Test Interferes with Johne’s Disease ELISA Diagnostics
- Author
-
Riona G. Sayers, John MacSharry, Jim O'Mahony, Aideen Kennedy, Rodney Govender, Ana T. Da Silva, and N. Byrne
- Subjects
lcsh:Immunologic diseases. Allergy ,Veterinary medicine ,Immunology ,Paratuberculosis ,Disease ,Enteritis ,Bovine tuberculosis ,Medicine ,Immunology and Allergy ,Original Research ,Mycobacteriacea ,biology ,Comparative test ,Johne’s disease ,business.industry ,medicine.disease ,biology.organism_classification ,Mycobacterium avium subspecies paratuberculosis ,TB test ,TB testing ,biology.protein ,Herd ,ELISA ,Antibody ,lcsh:RC581-607 ,business ,PPD - Abstract
Enzyme-linked immmunosorbent assays (ELISA) of milk and serum samples are a routinely used method of screening herds for Mycobacterium avium subspecies paratuberculosis (MAP). Infection with MAP causes a granulomatous enteritis of ruminants known as Johne’s disease (JD). The sensitivity (Se) and specificity (Sp) of MAP ELISAs leads to difficulties in the identification of both infected and infectious animals. Interference with MAP ELISA Se and Sp has been reported in MAP seronegative cows following administration of purified protein derivative (PPD) as part of intradermal testing for bovine tuberculosis (bTB). The aim of this study is to examine the impact of the single intradermal cervical comparative test (SICCT) for bTB, on both serum and milk MAP ELISA tests, in a herd containing both seropositive and seronegative cows pre- SICCT. A secondary objective is to provide appropriate timing of JD ELISA tests in relation to the SICCT. A herd of 139 cows were serum and milk sampled pre and post SICCT administration. Prior to SICCT, 6% of the herd tested seropositive for MAP using milk ELISA, with 8% positive on serum. ID Screen Paratuberculosis Indirect Screening Test (ID Vet) was used to screen the herd. Within 14 days of PPD administration, a significant increase in the prevalence of seropositive cows was recorded. Identical prevalence’s were recorded with both test matrices (39%). ELISA values remained significantly higher until day 43 post-SICCT in milk (P=0.850), and day 71 in serum (P=0.602). If the ‘new’ positives detected post-bTB testing are deemed false positives due to generation of cross-reacting antibodies by administration of PPD, milk would appear a more suitable sample for JD ELISA testing within two months of SICCT. In summary, sampling for JD utilising milk ELISA should be avoided in the 43 day period following PPD administration, with serum ELISA sampling avoided for an additional 28 days.
- Published
- 2014
- Full Text
- View/download PDF
6. Is TB Testing Associated With Increased Blood Interferon-Gamma Levels?
- Author
-
Kennedy AE, O'Mahony J, Byrne N, MacSharry J, and Sayers RG
- Abstract
The Republic of Ireland reports a relatively low prevalence of Johne's disease (JD) compared to international counterparts. Postulated reasons for this include a lower average herd size and a grass-based production system. Ireland also engages in high levels of bovine tuberculosis (bTB) testing. As interferon-gamma (IFN-γ) is believed to play a key role in protecting against JD, it is our hypothesis that administration of purified protein derivative (PPD), as part of the bTB test, is associated with a systemic increase in IFN-γ production, which may potentially limit clinical progression of the disease. We studied 265 cows (202 Friesian and 63 "Non-Friesian," e.g., JerseyX, Norwegian Red) to assess IFN-γ levels and Mycobacterium avium subspecies paratuberculosis (MAP) antibody response before and after the bTB test. As part of the compulsory annual bTB test, avian and bovine PPD were administered at two separate cervical sites. To assess IFN-γ production, blood samples were taken before and 72 h after PPD administration. MAP antibody response was assessed before and 10 days post-PPD administration. A significant increase in MAP antibody response was identified post-bTB compared to pre-bTB response ( p < 0.001). Additionally, IFN-γ production significantly increased at the post-bTB time point ( p < 0.001) compared to the pre-bTB test readings. This may indicate a beneficial effect of bTB testing in controlling JD.
- Published
- 2017
- Full Text
- View/download PDF
7. 27 People at Kansas High School Test Positive for Tuberculosis.
- Author
-
Frizell, Sam
- Abstract
More than 300 students and staff were tested for TB at the school [ABSTRACT FROM PUBLISHER]
- Published
- 2015
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