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2 results on '"style cysteine-rich adhesin"'

1. A multifaceted study of stigma/style cysteine-rich adhesin (SCA)-like Arabidopsis lipid transfer proteins (LTPs) suggests diversified roles for these LTPs in plant growth and reproduction

Author

Chae, Keun, Gonong, Benedict J, Kim, Seung-Chul, Kieslich, Chris A, Morikis, Dimitrios, Balasubramanian, Shruthi, and Lord, Elizabeth M

Subjects
Plant Biology, Biological Sciences, Antigens, Plant, Arabidopsis, Arabidopsis Proteins, Carrier Proteins, Cluster Analysis, Cysteine, Flowers, Gene Expression Regulation, Plant, Genes, Plant, Mutation, Phylogeny, Plant Proteins, Reproduction, Seedlings, Stress, Physiological, Structural Homology, Protein, Arabidopsis thaliana, electrostatics, extracellular matrix, lipid transfer protein, small secreted peptide, stigma, style cysteine-rich adhesin, Genetics, Crop and Pasture Production, Plant Biology & Botany, Crop and pasture production, Biochemistry and cell biology, Plant biology
Abstract

Lily stigma/style cysteine-rich adhesin (SCA), a plant lipid transfer protein (LTP) which is secreted into the extracellular matrix, functions in pollen tube guidance in fertilization. A gain-of-function mutant (ltp5-1) for Arabidopsis LTP5, an SCA-like molecule, was recently shown to display defects in sexual reproduction. In the current study, it is reported that ltp5-1 plants have dwarfed primary shoots, delayed hypocotyl elongation, various abnormal tissue fusions, and display multibranching. These mutant phenotypes in vegetative growth are recessive. No abnormality was found in ltp5-1/+ plants. In a phylogenetic analysis of plant LTPs, SCA-like Arabidopsis LTPs were classified with conventional plant LTPs. Homology modelling-based electrostatic similarity index (ESI) clustering was used to show diversity in spatial distributions of electrostatic potentials of SCA-like LTPs, suggestive of their various roles in interaction in the extracellular matrix space. β-Glucuronidase (GUS) analysis showed that SCA-like Arabidopsis LTP genes are diversely present in various tissues. LTP4 was found specifically in the guard cells and LTP6 in trichomes as well as in other tissues. LTP1 levels were specifically abundant in the stigma, and both LTP3 and LTP6 in the ovules. LTP2 and LTP4 gene levels were up-regulated in whole seedlings with 20% polyethylene glycol (PEG) and 300 mM NaCl treatments, respectively. LTP5 was up-regulated in the hypocotyl with 3 d dark growth conditions. LTP6 was specifically expressed in the tip of the cotyledon under drought stress conditions. The results suggest that SCA-like Arabidopsis LTPs are multifunctional, with diversified roles in plant growth and reproduction.

Published
2010

2. A multifaceted study of stigma/style cysteine-rich adhesin (SCA)-like Arabidopsis lipid transfer proteins (LTPs) suggests diversified roles for these LTPs in plant growth and reproduction

Author

Elizabeth M. Lord, Seung-Chul Kim, Chris A. Kieslich, Dimitrios Morikis, Benedict J. Gonong, Shruthi Balasubramanian, and Keun Chae

Subjects
0106 biological sciences, Arabidopsis thaliana, Physiology, Mutant, Arabidopsis, Plant Biology, Plant Science, 01 natural sciences, Hypocotyl, Gene Expression Regulation, Plant, Cluster Analysis, Phylogeny, style cysteine-rich adhesin, Plant Proteins, 0303 health sciences, biology, Reproduction, food and beverages, lipid transfer protein (LTP), Research Papers, Biochemistry, Pollen tube, Plant lipid transfer proteins, small secreted peptide, Cotyledon, stigma/style cysteine-rich adhesin (SCA), Crop and Pasture Production, food.ingredient, Physiological, extracellular matrix, Plant Biology & Botany, Flowers, Genes, Plant, Stress, 03 medical and health sciences, food, Stress, Physiological, Genetics, Cysteine, Antigens, 030304 developmental biology, Structural Homology, Arabidopsis Proteins, Protein, fungi, Plant, lipid transfer protein, Antigens, Plant, extracellular matrix (ECM), biology.organism_classification, electrostatics, Trichome, Sexual reproduction, Gene Expression Regulation, Genes, Structural Homology, Protein, Seedlings, stigma, Mutation, Carrier Proteins, 010606 plant biology & botany
Abstract

Lily stigma/style cysteine-rich adhesin (SCA), a plant lipid transfer protein (LTP) which is secreted into the extracellular matrix, functions in pollen tube guidance in fertilization. A gain-of-function mutant (ltp5-1) for Arabidopsis LTP5, an SCA-like molecule, was recently shown to display defects in sexual reproduction. In the current study, it is reported that ltp5-1 plants have dwarfed primary shoots, delayed hypocotyl elongation, various abnormal tissue fusions, and display multibranching. These mutant phenotypes in vegetative growth are recessive. No abnormality was found in ltp5-1/+ plants. In a phylogenetic analysis of plant LTPs, SCA-like Arabidopsis LTPs were classified with conventional plant LTPs. Homology modelling-based electrostatic similarity index (ESI) clustering was used to show diversity in spatial distributions of electrostatic potentials of SCA-like LTPs, suggestive of their various roles in interaction in the extracellular matrix space. β-Glucuronidase (GUS) analysis showed that SCA-like Arabidopsis LTP genes are diversely present in various tissues. LTP4 was found specifically in the guard cells and LTP6 in trichomes as well as in other tissues. LTP1 levels were specifically abundant in the stigma, and both LTP3 and LTP6 in the ovules. LTP2 and LTP4 gene levels were up-regulated in whole seedlings with 20% polyethylene glycol (PEG) and 300 mM NaCl treatments, respectively. LTP5 was up-regulated in the hypocotyl with 3 d dark growth conditions. LTP6 was specifically expressed in the tip of the cotyledon under drought stress conditions. The results suggest that SCA-like Arabidopsis LTPs are multifunctional, with diversified roles in plant growth and reproduction.

Published
2010

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