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Fungi have the potential to colonize soybean seeds in the field, during their maturation in the pods and after harvest, during storage. The aim of this study was to identify fungi inhabiting soybean seeds after storage with varying germination capacity and to evaluate their chemical composition. The research material consisted of twelve soybean seed lots collected from the fields in southern Poland and stored over winter. The germination percentage of these lots ranged between 20.67% and 81.33%. The seeds were subjected to analyses of the main chemical components and mycological analysis. Fungal isolates were subjected to taxonomic identification using microscopic methods and DNA sequencing (using internal transcribed spacer region and secondary barcoding regions). A total number of 355 fungal isolates from 16 genera were identified, with Aspergillus, Alternaria, and Fusarium being the most common. Species were successfully identified in 94% of isolates. Twelve examined seed lots varied significantly in the number of isolated fungal species (from 1 to 17). Moreover, they also differed in the isolated species composition. Highly significant positive correlation was found between the number of Aspergillus psedudoglaucus isolates and the content of free fatty acids. In turn, the number of Fusarium spp. isolates correlated negatively with protein and nitrogen content. Similarly, highly significant negative correlation was found between the number of all fungal isolates and the 1000-seed weight, indicating that smaller seeds are more vulnerable to fungal infection. The results obtained in this study identify species of fungi which may be responsible for lowering quality of the seeds obtained in southern Poland. [ABSTRACT FROM AUTHOR]

Fungi have the potential to colonize soybean seeds in the field, during their maturation in the pods and after harvest, during storage. The aim of this study was to identify fungi inhabiting soybean seeds after storage with varying germination capacity and to evaluate their chemical composition. The research material consisted of twelve soybean seed lots collected from the fields in southern Poland and stored over winter. The germination percentage of these lots ranged between 20.67% and 81.33%. The seeds were subjected to analyses of the main chemical components and mycological analysis. Fungal isolates were subjected to taxonomic identification using microscopic methods and DNA sequencing (using internal transcribed spacer region and secondary barcoding regions). A total number of 355 fungal isolates from 16 genera were identified, with Aspergillus, Alternaria, and Fusarium being the most common. Species were successfully identified in 94% of isolates. Twelve examined seed lots varied significantly in the number of isolated fungal species (from 1 to 17). Moreover, they also differed in the isolated species composition. Highly significant positive correlation was found between the number of Aspergillus psedudoglaucus isolates and the content of free fatty acids. In turn, the number of Fusarium spp. isolates correlated negatively with protein and nitrogen content. Similarly, highly significant negative correlation was found between the number of all fungal isolates and the 1000-seed weight, indicating that smaller seeds are more vulnerable to fungal infection. The results obtained in this study identify species of fungi which may be responsible for lowering quality of the seeds obtained in southern Poland.

One of the most common pests in stored grain is Aspergillus. This group of fungi produces a carcinogenic toxin, Aflatoxin during their growth and development. Contamination of Aspergillus in food grains during storage leads to food insecurity. In the present-day scenario, using plant-based derivatives in controlling Aspergillus is one of the efficient and eco-friendly ways. Hence an in silico study was carried out to know the effective phytochemicals present in Citrus, Carum carvi, Coriander sativum, Aloysia citriodora, Mentha citrate, Spent hops, Nardostachys jatamansi, Feoniculum vulgare, Zingiber officinale, Lantana camara, Chamaecyparis obtusa, Ocimum kilimandscharium, Tagetes filifolia against Aspergillus. Results revealed that the photochemicals viz. Eugenol, zingiberene, carvone, citronellal, limonene, coumaran, linalool, linalyl acetate, esdragol, menthol, E-anethole, camphor, bornyl acetate, xanthohumol and aristolone present in the selected plants can inhibit the normal functioning of Ypd1 protein of Aspergillus by blocking its active site. Thus, the present study makes a base for future researchers to find the most effective phytochemicals in controlling Aspergillus following in vivo method. [ABSTRACT FROM AUTHOR]

Abstract Cowpea is one of the main crops in family agriculture, especially in the Northeastern region of Brazil, and it is expanding to other regions in Brazil. The use of seeds with low physiological and health quality is reflected in the plant development and consequently yield, making it important to study the seed physiological and health quality. The objective of the present study was to assess the physiology and health of traditional and biofortified cowpea seeds. The traditional cowpea varieties (Angelim, Mercado and Manteguinha) and the biofortified cowpea cultivars (BRS Aracê, BRS Xique-Xique and BRS Tumucumaque) were assessed for the following physiological parameters: water content (WC), first count (FC), germination test (G), germination speed index (GSI), seedling emergence in the greenhouse (E), emergence speed index (ESI), seedling aerial part and root length (APL and RL) and electric conductivity test (EC). The seed health quality was assessed by the Blotter Test. The water content present in the seeds of the traditional and biofortified varieties ranged from 10% to 14%. All the traditional and biofortified varieties showed high germination and emergence value in the greenhouse. The germination and emergence speed indexes indicated the BRS Aracê and BRS Xique-Xique cultivars as the most vigorous. In the health tests the highest indexes were the storage fungi Aspergillus sp., Cladosporium sp. and Penicillium sp., with the highest prevalence in the BRS Tumucumaque variety, which was probably related to the higher water content present in this variety.

Introduction: Milk thistle (Silybum marianum (L.) Gaertn.) is an important medicinal plant, its achenes contain a silymarin, which has a regenerating and stabilizing effect on liver cells. The milk thistle based dietary supplements are among the best-selling plant pharmaceuticals in the US and the UE.

Aspergillus flavus is a frequent contaminant of maize grain. We isolated this fungus, determined the colony morphology and species (by internal transcribed spacer sequencing) and measured the aflatoxin content. The selected A. flavus fungi were placed into two groups, toxigenic and atoxigenic; both appeared similar morphologically, except that the atoxigenic group lacked sclerotia. An essential oil fumigation test with clove and cinnamon oils as antifungal products was performed on fungal conidial discs and fungal colonies in Petri plates. Cinnamon oil at 2.5 to 5.0 μL/plate markedly inhibited the mycelial growth from conidial discs of both strains, whereas clove oil showed less activity. The oils had different effects on fungal mycelia. The higher clove fumigation doses of 10.0 to 20.0 μL/plate controlled fungal growth, while cinnamon oil caused less inhibition. Compared with atoxigenic groups, toxigenic A. flavus responded stably. Within abnormal A. flavus hyphae, the essential oils degenerated the hyphal morphology, resulting in exfoliated flakes and shrinkage, which were related to fungal membrane injury and collapse of vacuoles and phialide. The treatments, especially those with cinnamon oil, increased the electroconductivity, which suggested a weak mycelium membrane structure. Moreover, the treatments with essential oils reduced the ergosterol content in mycelia and the aflatoxin accumulation in the culture broth. The fumigations with clove and cinnamon oils inhibited the development of both conidia and colonies of A. flavus in dose-dependent manners.

Aspergillus flavus is a frequent contaminant of maize grain. We isolated this fungus, determined the colony morphology and species (by internal transcribed spacer sequencing) and measured the aflatoxin content. The selected A. flavus fungi were placed into two groups, toxigenic and atoxigenic; both appeared similar morphologically, except that the atoxigenic group lacked sclerotia. An essential oil fumigation test with clove and cinnamon oils as antifungal products was performed on fungal conidial discs and fungal colonies in Petri plates. Cinnamon oil at 2.5 to 5.0 μL/plate markedly inhibited the mycelial growth from conidial discs of both strains, whereas clove oil showed less activity. The oils had different effects on fungal mycelia. The higher clove fumigation doses of 10.0 to 20.0 μL/ plate controlled fungal growth, while cinnamon oil caused less inhibition. Compared with atoxigenic groups, toxigenic A. flavus responded stably. Within abnormal A. flavus hyphae, the essential oils degenerated the hyphal morphology, resulting in exfoliated flakes and shrinkage, which were related to fungal membrane injury and collapse of vacuoles and phialide. The treatments, especially those with cinnamon oil, increased the electroconductivity, which suggested a weak mycelium membrane structure. Moreover, the treatments with essential oils reduced the ergosterol content in mycelia and the aflatoxin accumulation in the culture broth. The fumigations with clove and cinnamon oils inhibited the development of both conidia and colonies of A. flavus in dose-dependent manners. [ABSTRACT FROM AUTHOR]

Sweet chestnut fruits are popular fruits commercialized as fresh or processed ready-to-eat products. The major post-harvest problems associated with stored chestnut fruits are fungal rots, which cause major losses in fruit quality. The aims of this work were to determine the incidence, abundance and diversity of rots and fungi in three chestnut varieties (Longal, Judia and Martaínha) of Portugal, collected from an industrial plant, and to identify the stages of storage and processing where fungi and rots are more significant. Thirty-three chestnut samples from the three varieties were collected from different stages of industrial processing. Nuts were internally and externally inspected for damage, infestation and infection, and internal fungi were isolated and molecularly identified. The variety Martaínha was identified as the least susceptible to fungal growth, while Longal was the most susceptible. A high diversity of fungi was detected and identified. The dominant fungi were Mucor racemosus, Penicillium spp. (the causal agents of green rots), Ciboria batschiana (black rot) and Botrytis cinerea (gray rot). Gnomoniopsis smithogilvyi, the causal agent of brown rot, was also frequently detected. Sterilization with hydrothermal bath was effective in the elimination or reduction of most of the rot-causing fungi. These results could serve as a baseline for better monitoring fungal development and chestnut decay, and to develop effective management measures to control post-harvest chestnut rots.

Antifungal activity of extracts of cinnamon (Cinnamomum zeylanicum), Cloves (Syzygium aromaticum), ginger (Zingiber officinale) and turmeric (Curcuma longa) were evaluated in vitro against 17 Penicillium spp. Seed disease and rotten fruit caused by these species cause considerable loss of quality for different agricultural products. Isolates of Penicillium spp. were screened for production of patulin an important serious mycotoxin. About 70.59% of Penicillium spp. produced this toxin in concentrations ranging from 4 to 31 ppb. The response of Penicillium spp. to plant extracts differed according to the plant extract and concentration. Cinnamon extract showed the greatest effect on P. asperosporum, P. aurintogriseum and P. brevicompactum , and cloves extract produced the greatest effect on P. chermesinum and P. duclauxii. Turmeric extract had less effect on P. duclauxii. Cloves extract was the most effective in reducing the growth of Penicillium spp. On the other hand, ginger extract with all concentrations used had less effect against most Penicillium spp in the laboratory. Plant extracts are promising as natural sources of environmentally friendly compounds in laboratory studies. [ABSTRACT FROM AUTHOR]

DOR: 98.1000/2383-1251.1398.6.65.11.1. 1578.1585 Extended Abstract Introduction: Understanding the complex characteristics that control the life span of the seed has ecological, agricultural and economic importance. Inappropriate storage conditions after harvesting destroy a large part of annual yield partly due to microbial activity in the storage. Damage from storage fungi varies based on the climatic conditions, crops and storage facilities. This study was carried out to investigate the effect of storage conditions and initial seed moisture content on the growth of storage fungi and also the relationship between the degree of contamination with fungi and the quality and biochemical changes of the seeds. Materials and Methods: The present study was carried out as a factorial experiment based on a completely randomized design to assess the impact of storage fungi on soybean seed deterioration at different storage conditions. The treatment included three degrees of initial seed moisture content including low moisture content (10%), medium moisture content (12%) and high moisture content (14%) as the first factor. Moreover, two storage conditions including the seed storage in Moghan and controlled seed storage in Seed and Plant Certification and Registration Institute were considered as the second factor. Soybean seeds of Williamschr('39')s cultivar were investigated for the infection of Aspergillus flavus, Aspergillus niger, Fusarium and Penicillium fungi and also related biochemical traits and seed quality such as germination percent, seedling vigor index, soluble sugar and total protein. Results: The results of this experiment showed that the increase of the seed moisture content by 14% can significantly decrease the seed quality. Therefore, the seed moisture content of 14% was identified as unsuitable moisture for the storage of soybean seeds. In addition, the infection with storage fungi has a direct relationship with the degree of seed moisture and seeds with high moisture content are rapidly attacked by the storage fungi which can decrease seed quality and viability. Moreover, the Aspergillus niger infection increased from 27.5 to 43.75 and the germination percent decreased from 52.5 to 23 percent in seeds with a moisture content of 14% in Moghan storage, as compared with the controlled storage. Furthermore, this study showed that when the percentage of storage fungi increases, the soybean seed deterioration increases. Studying the biochemical changes of deteriorated seeds during the storage showed that as the aging of the seeds increases, soluble sugars and protein percentage decrease. The amounts of soluble sugars and total protein of the seed were significantly lower in seeds maintained under unsuitable conditions. Furthermore, the content of soluble sugars and total protein decreased significantly by the increase of the seed moisture, which resulted in the increase in seed deterioration. Conclusions: Based on the obtained results, initial seed moisture and storage conditions are two important determinants of fungi infestation during storage, which can affect the content of soluble sugars and total protein causing seed deterioration, seed vigor and viability. It can be concluded that the soybean seed moisture content of 12%, which is the standard moisture content of soybean seed production in Iran, is regarded as suitable moisture for seed storage. Highlights: Introduction of proper storage conditions and initial seed moisture in order to decrease fungal damage and soybean seed deterioration. Determination of different fungal damages during the storage of soybean seeds. Determination of relationship between the degree of soybean seed infection of storage fungi and the seed’s quality, its amount of protein and soluble sugars.

Presence of fungi causing infestations deteriorates quality of husked and dehisced rice (Oryza sativa L.) seed. So, identifying storage conditions is crucial in food safety programs. Present study deals with isolation of fungi and their purification from rice to know the impact on seed storage quality. Four important fungi causing infestations viz. Aspergillus flavus, A. niger, Penicillium sp. and Fusarium sp. were isolated and characterized. Culture filtrate of fungi were studied on rice seed deterioration on the basis of seed germination percentage, root/shoot length, total carbohydrate, total protein content, α-amylase activity, lipoxygenase activity and storage proteins profiling by SDSPAGE. Maximum deterioration in rice seed was observed with A. flavus spores filtrate inoculated seeds followed by A. niger, Penicillium sp. and Fusarium sp., respectively. Enzyme activity of α-amylase and lipoxygenase were significantly increased with seed storage and their ageing.

In this study, we evaluated the effect of different temperatures (10, 20, 30, and 40 °C) and relative humidities (RHs; 12, 44, 76, and 98%) on populations of predominant grain fungi (Aspergillus candidus, Aspergillus flavus, Aspergillus fumigatus, Penicillium fellutanum, and Penicillium islandicum) and the biocontrol activity of Pseudomonas protegens AS15 against aflatoxigenic A. flavus KCCM 60330 in stored rice. Populations of all the tested fungi in inoculated rice grains were significantly enhanced by both increased temperature and RH. Multiple linear regression analysis revealed that one unit increase of temperature resulted in greater effects than that of RH on fungal populations. When rice grains were treated with P. protegens AS15 prior to inoculation with A. flavus KCCM 60330, fungal populations and aflatoxin production in the inoculated grains were significantly reduced compared with the grains untreated with strain AS15 regardless of temperature and RH (except 12% RH for fungal population). In addition, bacterial populations in grains were significantly enhanced with increasing temperature and RH, regardless of bacterial treatment. Higher bacterial populations were detected in biocontrol strain-treated grains than in untreated control grains. To our knowledge, this is the first report showing consistent biocontrol activity of P. protegens against A. flavus population and aflatoxin production in stored rice grains under various environmental conditions of temperature and RH.

Grain storage plays a crucial role in ensuring food security to Thai farmers so sustainable protection methods against deleterious microorganisms, such as fungi, are necessary. Essential oils (EOs) have demonstrated broad-spectrum fumigant antifungal activity against most storage fungi that are problematic in Thailand. Four storage fungi (Aspergillus flavus, A. niger, Curvularia lunata and Fusarium proliferatum) were isolated from dried rice and corn grains (stored for at least six months). EOs were extracted by hydrodistillation from clove buds (Syzygium aromaticum), fruit peel and leaves of makrut lime (Citrus hystrix), eucalyptus leaves (Eucalyptus sp.) and lemongrass stems (Cymbopogon citratus). The fungi inoculated on PDA in plastic cups were exposed to each EO vapour originating from paper disc attached in the lids in an inverted position. The minimum inhibitory concentration (MIC) for each EO was determined. Selected MICs were combined in a binary manner and similarly tested against the fungi. Fractional inhibitory concentration indices (FICI) were determined for each combination. Lemongrass and makrut lime leaf EOs were the most effective with MICs of 0.09 µL/mL against Curvularia lunata and 0.19-0.28 µL/mL against A. flavus, A. niger and F. proliferatum. Eucalyptus oil produced the least effective vapour (MIC 0.56-0.74 µL/mL) against all tested pathogens. A combination of lemongrass and makrut lime leaf EOs was partially synergistic against A. niger (FICI=0.75) but was fully synergistic against the other three fungi tested (FICI=0.5). The EOs from lemongrass and makrut lime leaf have potential to suppress the growth of the four grain-storage fungi tested. [ABSTRACT FROM AUTHOR]

ABSTRACT Fungi of the genus Aspergillus may promote damage to the physiological quality of forest seeds, and plant extracts have been used to control microflora associated with these seeds. Thus, the aim of the present work was to perform the phytochemical characterization of ethanolic and aqueous extracts obtained from Eugenia dysenterica leaves (cagaita), as well as to evaluate the effect of these extracts on the development of Aspergillus sp. isolated from Hymenaea stigonocarpa seeds (jatobá-do-cerrado). For this, qualitative phytochemical analysis of extracts was carried out and their effect on Aspergillus sp mycelial growth, sporulation and spore germination was evaluated. As a result, phenols, hydrolysable tannins, flavonols, flavanones, xanthones, flavones, free steroids and saponins were found in both extracts, which could be used in the search for new antifungal compounds in the treatment of stored forest seeds, since extracts have secondary compounds that allow inhibiting Aspergillus sp mycelial growth.

Studies were carried out to determine the occurrence of storage fungi and their respective mycotoxin in stored smoked dried fishes. A total of 45 fishes were bought fresh, smoked and stored under three different storage conditions (an air tight container, a bamboo basket and an iron basket). Using the pour plate method, isolation and identification of storage fungi were carried out weekly for four consecutive weeks from each storage container. Mycotoxin detection and quantification were also carried out on each stored smoked fish samples using Agra-Quant kit. The proximate analysis of the stored fish samples prior to storage, and after storage from each storage container was also analysed. Results revealed five fungal isolates associated with the stored samples namely: Aspergillus flavus, A. fumigatus, A. niger, A. wentii and Penicillium species. Four species of Aspergillus and one Penicillium species were isolated and identified from two of three storage condition used (bamboo basket and iron basket) from third week of storage. It was deduced from the mycotoxins analysis results that Aflatoxin B1 and Ochratoxin were present in the stored smoked samples of the two above mentioned storage containers. Results from the proximate compositions revealed a decrease in the ash content, crude fiber and crude protein as the storage period increases. Fat and moisture content of the stored fish samples were observed to increases weekly throughout the storage period in bamboo basket and an iron basket. In conclusion, it can be deduced that storage container play a vital role in the preservation/shelf -life of smoked dried fish.

Structural characteristics, curve dehydration, dry matter production, chemical composition, occurrence of fungi, temperature in the storage of hay bales of Tifton 85 oversowing with long-cycle oat (IPR 126) and oats for short-cycle grains production (Guapa), were evaluated. For chemical composition, the experimental design used was randomized blocks, a split-plot overtime and 3 treatments (single Tifton 85, Tifton 85 with IPR 126 oat oversowing and Tifton 85 in association with Guapa white oat and 3 assessment periods (cutting, baling and 30 days of storage) with 5 repetitions. The dry matter production was higher in single Tifton 85 compared to associations with white oat. The largest stem diameter of guapa white oat contributed to reduce losses of water in the dehydration process, with the hay in this culture system being stored under 800 g kg-1 DM. It was found that the crude protein did not differ between cropping systems, but Tifton 85 hay showed high levels of acid detergent insoluble protein and higher levels of NDF and lignin. The occurrence of fungi was higher after storage predominating fungi of the genus Penicillium.

The incidence and severity of Aspergillus ochraceus can reduce seed quality. In this study, we aimed to study the physiological quality of soybean seeds naturally infected with A. ochraceus. Seeds of soybean cv. Conquest with different levels of inoculum of A. ochraceus were obtained by the technique of water and separated into three classes (N0, N1 and N2) according to the constraint surface area covered with fungal structures. The control samples were taken from the N0 treatment, two samples of 200 soybean seeds, the first being treated with fungicides carboxin + thiram and the second sample subjected to surface disinfestation by immersion in a solution of sodium hypochlorite. Next, we assessed the vigour of seeds through the emergency test in trays and the tetrazolium test. The length, seedling fresh and dry weight of shoot and root were evaluated at 21 days after sowing. There was a reduction in the rate of speed of emergence and seedling emergence in the seeds with levels of up to 50 % of inoculum compared to treatment with fungicide. The length of the seedlings (44 %), fresh weight of shoot and root (82 %), dry weight of shoots (81 %) and root dry mass (76 %) were also reduced . Soybean seeds with incidence above 50 % of A. ochraceus showed total loss of vigor and decay of tissues.

In this study, we evaluated the effect of different temperatures (10, 20, 30, and 4 0 °C) and relative humidities (RHs; 12, 44, 76, and 98%) on populations of predominant grain fungi (Aspergillus candidus, Aspergillus flavus, Aspergillus fumigatus, Penicillium fellutanum, and Penicillium islandicum) and the biocontrol activity of Pseudomonas protegens AS15 against aflatoxigenic A. flavus KCCM 60330 in stored rice. Populations of all the tested fungi in inoculated rice grains were significantly enhanced by both increased temperature and RH. Multiple linear regression analysis revealed that one unit increase of temperature resulted in greater effects than that of RH on fungal populations. When rice grains were treated with P. protegens AS15 prior to inoculation with A. flavus KCCM 60330, fungal populations and aflatoxin production in the inoculated grains were significantly reduced compared with the grains untreated with strain AS15 regardless of temperature and RH (except 12% RH for fungal population). In addition, bacterial populations in grains were significantly enhanced with increasing temperature and RH, regardless of bacterial treatment. Higher bacterial populations were detected in biocontrol strain-treated grains than in untreated control grains. To our knowledge, this is the first report showing consistent biocontrol activity of P. protegens against A. flavus population and aflatoxin production in stored rice grains under various environmental conditions of temperature and RH. [ABSTRACT FROM AUTHOR]

Studies were carried out to determine the occurrence of storage fungi and their respective mycotoxin in stored smoked dried fishes. A total of 45 fishes were bought fresh, smoked and stored under three different storage conditions (an air tight container, a bamboo basket and an iron basket). Using the pour plate method, isolation and identification of storage fungi were carried out weekly for four consecutive weeks from each storage container. Mycotoxin detection and quantification were also carried out on each stored smoked fish samples using Agra-Quant kit. The proximate analysis of the stored fish samples prior to storage, and after storage from each storage container was also analysed. Results revealed five fungal isolates associated with the stored samples namely: Aspergillus flavus, A. fumigatus, A. niger, A. wentii and Penicillium species. Four species of Aspergillus and one Penicillium species were isolated and identified from two of three storage condition used (bamboo basket and iron basket) from third week of storage. It was deduced from the mycotoxins analysis results that Aflatoxin B1 and Ochratoxin were present in the stored smoked samples of the two above mentioned storage containers. Results from the proximate compositions revealed a decrease in the ash content, crude fiber and crude protein as the storage period increases. Fat and moisture content of the stored fish samples were observed to increases weekly throughout the storage period in bamboo basket and an iron basket. In conclusion, it can be deduced that storage container play a vital role in the preservation/shelf-life of smoked dried fish. [ABSTRACT FROM AUTHOR]

Maize plays a key role in household food security in Ethiopia, but its benefit has been limited with high post-harvest losses. This study was initiated to assess post-harvest practices and associated fungi pathogen epidemiology along the maize supply chain in southwestern Ethiopia. The study was conducted in five purposively selected districts and a three-stage sampling procedure was employed for selection of the target groups. In total, 342 participants from different actors were interviewed using a semi-structured questionnaire. Maize samples were collected every month from 63 randomly selected actors for mycological analysis during six months storage period. Post-harvest loss was estimated to be 31% and loss during storage was identified as a critical loss point. Comparing all biological agents, loss due to fungal pathogens in the store ranked on top. Moisture content at loading stage could not increase the shelf life of the commodity. Germination tests showed a significant (P < 0.01) decrease as storage duration increased, while mould incidence on cobs and kernels significantly (P < 0.05) increased. In total, seven fungal genera were isolated, characterized and identified, with Fusarium, Penicillium and Aspergillus being predominant. Most of the post-harvest practices are not effective in reducing post-harvest losses. Especially, farmers’ traditional storage structures can be influenced by external climatic conditions and make the grains liable to develop mould during the rainy season. This research, therefore, highlights the need to design, develop or modify existing storage technologies that reduce post-harvest loss due to mycotoxin-producing fungal pathogens. Furthermore, postharvest drying to obtain optimum moisture content is also crucial to reduce losses. [ABSTRACT FROM AUTHOR]

Cereal grains are the most important food source for humans. As the global population continues to grow exponentially, the need for the enhanced yield and minimal loss of agricultural crops, mainly cereal grains, is increasing. In general, harvested grains are stored for specific time periods to guarantee their continuous supply throughout the year. During storage, economic losses due to reduction in quality and quantity of grains can become very significant. Grain loss is usually the result of its deterioration due to fungal contamination that can occur from preharvest to postharvest stages. The deleterious fungi can be classified based on predominance at different stages of crop growth and harvest that are affected by environmental factors such as water activity (aw) and eco-physiological requirements. These fungi include species such as those belonging to the genera Aspergillus and Penicillium that can produce mycotoxins harmful to animals and humans. The grain type and condition, environment, and biological factors can also influence the occurrence and predominance of mycotoxigenic fungi in stored grains. The main environmental factors influencing grain fungi and mycotoxins are temperature and aw. This review discusses the effects of temperature and aw on fungal growth and mycotoxin production in stored grains. The focus is on the occurrence and optimum and minimum growth requirements for grain fungi and mycotoxin production. The environmental influence on aflatoxin production and hypothesized mechanisms of its molecular suppression in response to environmental changes are also discussed. In addition, the use of controlled or modified atmosphere as an environmentally safe alternative to harmful agricultural chemicals is discussed and recommended future research issues are highlighted. [ABSTRACT FROM AUTHOR]

Eleven plant extracts, viz. Allium sativum (garlic), Adhatoda vasica (malabar nut), Vitex negundo (horseshoe vitex), Melia azadirachta (chinaberry tree), Tagetes erecta (aztec marigold), Cannabis sativa (cannabis), Lantana camara (lantana), Mentha piperata (Pepper mint), Curcuma longa (turmeric), Zingiber officinale (ginger) and Allium cepa (onion) were evaluated for their efficacy against storage rot fungi, germination percentage and seedling vigour of pea. All the plant extracts significantly reduced the storage rot fungi, i.e. Aspergillus sp., Alternaria alternata, A. solani, Phoma sp and Fusarium spp.; increased seed germination and seedling vigour index of pea. Vitex negundo and Melia azadirachta found equally effective with minimum storage rot of 1.94%, while the maximum storage rot was observed in control (13.84%) where no seed treatment was done. Maximum seed germination (89.08%) was found in the seed stored in cloth bags, while in plastic bags seed germination found was 88.40 per cent. Maximum germination (96.41%) was recorded in the seed treated with Mentha piperata, followed by the Tagetes erecta with 94.08 per cent seed germination. Maximum seedling length and dry matter was observed in Allium sativum treated seeds with 28.33 cm and 2.66 g. Maximum (2520) seedling vigour I was observed in Mentha piperata, similarly maximum (308) seedling vigour II was recorded in Mentha piperata. Minimum (2.37%) population of the mycoflora was obtained in seed treated with the Curcuma longa when stored in plastic boxes. Lowest (3.28%) mycoflora was observed in Vitex negundo treated seed when stored in cloth bags. Aspergillus sp. is the most frequently occurring fungi in storage of pea. Pea seeds developed less attack by storage fungi particularly with Alternaria alternata and A. solani while more association of Aspergillus spp., Phoma sp. and Fusarium spp. were observed with the seeds during storage period in different containers. However fewer microflora (16.40%) developed in cloth bags compared to plastic boxes (20.56%).

The study was conducted to estimate the dehydration curves, chemical composition, and occurrence of fungi in white oat hay (Avena sativa L. cv. Guapa BRS) and black oat hay (Avena strigosa Schreb cv. Common) at two cutting heights. Dehydration curves were studied under a randomized block design with a 2 × 2 factorial arrangement using split plots in time, considering two types of oats (white and black), two cutting heights (10 and 20 cm), and 17 sampling times (0, 4, 19, 24, 28, 43, 47, 52, 67, 71, 76, 91, 95, 100, 115, 129, and 124 hours after harvesting) with five replicates. For the chemical composition and occurrence of fungi, the experimental design comprised randomized blocks in a factorial 2 × 2 split-plot in time with two types of oats, two cutting heights, and three assessment periods: before cutting, during baling, and after 30 days of storage, with five replicates. The hay obtained by cutting of the black and white oats at heights of 10 and 20 cm showed similar dehydration curves. The crude protein values were higher in white oats only at the time of cutting (141.5 g/kg). The black oats showed lower nutritional quality, with higher levels of ADF and lignin. There was no effect of cutting height on the chemical composition, but the cutting height interfered with the production of dry matter and residue after cutting, with cutting at 10 cm leading to higher dry matter production and at 20 cm to increased waste production. The cutting heights of the oats interfere directly with the dry matter production and post-harvest residue without changing the chemical composition of the hay.

Mycotoxins are commonly present in feed for farm animals. Sows and gilts are highly susceptible to mycotoxins. This article presents a review describing the main mycotoxins encountered in pig feed which have a negative impact on sow fertility and reproduction. Consumption of feed that is contaminated with these mycotoxins may cause a variety of symptoms, depending on the type of mycotoxin, quantity and duration of exposure, as well as the health status and condition of the animal at the time of exposure. Two types of fungi are recognized, field fungi and storage fungi. Field fungi such as Fusarium spp., Aspergillus spp. and Claviceps spp. may produce toxins that lead to disturbed reproductive performance. Storage fungi occur if the humidity during storage is too high. In daily practice, the symptoms related to mycotoxicosis can occur at toxin concentrations below the detection limit. Knowledge of the effects of mycotoxins is expanding rapidly. Mycotoxins may still be present in feedstuffs despite negative analytical findings and because of the presence of hot spots in feed and or feedstuffs. Clinical symptoms can be very pronounced, making the diagnosis for the practitioner quite easy but in many cases the symptoms are vague and not at all present at herd level on a regular basis. The practitioner is in the first line of raising awareness in all parties whenever the first indication exists of a possible mycotoxicosis problem causing reproductive failure in breeding pigs. The problems can be resolved only if all parties involved in pig herd health take the necessary preventive measures and actions. The main toxins causing reproductive failure discussed in this article are aflatoxins, ergot alkaloids, trichothecenes and zearalenone.

Thirty-five randomly collected samples of stored table grapes ( Vitis vinifera L.) from different markets of Gorakhpur city, Uttar Pradesh, India, revealed occurrence of 11 types of fungi. Of which, Aspergillus flavus, Aspergillus niger, and Aspergillus ochraceus were dominant causing severe decay of grapes with 58%, 52%, and 67% incidence, respectively. On screening of 15 essential oils at 0.33 μL/mL, Cymbopogon citratus oil caused 100% mycelial inhibition against aforesaid dominant fungi. Oil was fungistatic at 0.29 μL/mL and exhibited broad fungitoxicity against other fruit rotting fungi associated with collected samples. C. citratus oil completely inhibited the growth and mycotoxin (AFB1 and OTA) secretion of the aflatoxigenic and ochratoxigenic strains of A. flavus, A. niger, and A. ochraceus at 0.8 μL/mL. E-Citral (52.9%) and Z-Citral (39.38%) were the major components of C. citratus oil during gas chromatography and gas chromatography-mass spectrometry analysis. Application of 200 and 300 μL of C. citratus oil on 1 kg of stored grapes showed enhancement of shelf life up to 10 d. The oil did not exhibit any phytotoxic effect on fruits. These results confirm that C. citratus oil could be a natural alternative to commercial fungicide for control of fruit rotting fungi of stored grapes. [ABSTRACT FROM AUTHOR]

This study was conducted to detection fungi associated with some condiment were selling in local market such as Cumin, Anise, Cinnamon, Sesame, Fennel, Black pill, nutmeg, coriander, Black pepper, Turmeric, Ginger, Galangal, caraway, cardamom. Results were recorded twenty three fungus according to fifteen genus included pathogenic fungi, saprophytic fungi and storage fungi [ABSTRACT FROM AUTHOR]

Almonds ( Prunus dulcis (Miller) D.A. Webb) and European (sweet) chestnuts ( Castanea sativa Miller) are of great economic and social impact in Mediterranean countries, and in some areas they constitute the main income of rural populations. Despite all efforts to control fungal contamination, toxigenic fungi are ubiquitous in nature and occur regularly in worldwide food supplies, and these nuts are no exception. This work aimed to provide knowledge on the general mycobiota of Portuguese almonds and chestnuts, and its evolution from field to the end of storage. For this matter, 45 field chestnut samples and 36 almond samples (30 field samples and six storage samples) were collected in Trás-os-Montes, Portugal. All fungi belonging to genus Aspergillus were isolated and identified to the section level. Fungi representative of other genera were identified to the genus level. In the field, chestnuts were mainly contaminated with the genera Fusarium, Cladosporium, Alternaria and Penicillium, and the genus Aspergillus was only rarely found, whereas almonds were more contaminated with Aspergillus. In almonds, Aspergillus incidence increased significantly from field to the end of storage, but diversity decreased, with potentially toxigenic isolates belonging to sections Flavi and Nigri becoming more significant and widespread throughout storage. These fungi were determined to be moderately associated, which can be indicative of mycotoxin co-contamination problems if adequate storage conditions are not secured. [ABSTRACT FROM AUTHOR]

Abstract: Essential oils (EOs) from boldo [Pëumus boldus Mol.], poleo [Lippia turbinata var. integrifolia (Griseb.)], clove [Syzygium aromaticum L.], anise [Pimpinella anisum] and thyme [Thymus vulgaris]) obtained by hydrodistillation were evaluated for their effectiveness against the growth of Aspergillus niger aggregate and A. carbonarius and accumulation of ochratoxin A (OTA). The evaluation was performed by compound dissolution at the doses of 0, 500, 1500 and 2500μL/L in peanut meal extract agar (PMEA) and exposure to volatiles of boldo, poleo (0, 1000, 2000 and 3000μL/L) and clove oils (0, 1000, 3000 and 5000μL/L), taking into account the levels of the water activity of the medium (aW 0.98, 0.95, 0.93). Statistical analyses on growth of Aspergillus strains indicated that the major effect was produced by oil concentrations followed by substrate aW, and that reductions in antifungal efficiency of the oils tested were observed in vapor exposure assay. At all aW levels, complete fungal growth inhibition was achieved with boldo EO at doses of 1500 and 2000μL/L by contact and volatile assays, respectively. Contact exposure by poleo and clove EOs showed total fungal inhibition at the middle level tested of 1500μL/L, regardless of aW, while their antifungal effects in headspace volatile assay were closely dependent on medium aW. The fumigant activity of poleo (2000μL/L) and clove oils (3000μL/L) inhibited growth rate by 66.0% and 80.6% at aW 0.98 and 0.93, respectively. OTA accumulation was closely dependent on aW conditions. The antiochratoxigenic property of the volatile fractions of boldo, poleo and clove EOs (1000μL/L) was more significant at low aW levels, inhibition percentages were estimated at 14.7, 41.7 and 78.5% at aW 0.98, 0.95 and 0.93, respectively. Our results suggest that boldo, poleo and clove oils affect the OTA biosynthesis pathway of both Aspergillus species. This finding leaves open the possibility of their use by vapor exposure as effective non-toxic biopreservatives against OTA contamination in stored peanuts. [Copyright &y& Elsevier]

The essential oil of Artabotrys odoratissimus R.Br. was evaluated for antifungal activity against some storage fungi causing contamination of food stuffs. Minimum inhibitory concentration of the oil was found to be 750 μL L−1 against Aspergillus flavus Link. It was found superior over different prevalent synthetic fungicides which inhibited the growth of A. flavus between 1000–5000 μL L−1. The oil exhibited broad fungitoxic spectrum against fourteen different storage fungi. Aspergillus fumigatus was inhibited at 1000 μL L−1 whereas Cladosporium cladosporioides, Curvularia lunata, Fusarium oxysporum, Helminthosporium oryzae, Macrophomina phaseolina, Microsporum gypseum, Mucor racemosus, Penicillium italicum, Pythium debaryanum, Rhizoctonia solani, Sclerotium rolfsii and Trichoderma viride at 500 μL L−1. Aspergillus niger was found to be inhibited only 84.9% at 1000 μL L−1. In addition, the oil showed significant efficacy in arresting aflatoxin B1 secretion by the toxigenic strain (Navjot 4NSt) of A. flavus at 750 μL L−1. The efficacy of A. odoratissimus oil as aflatoxin suppressor is being reported for the first time. [ABSTRACT FROM AUTHOR]

The in vitro antifungal effect of the essential oil of Citrus medica L. on storage fungi of Arachis hypogea L. stored for 6 months was evaluated using the disc diffusion agar method. The oil exhibited a wide spectrum of fungitoxicity, inhibiting all 14 fungus species tested. Thus, the oil can be exploited as a fumigant against storage fungi for the preservation of stored legume seeds due to its wide range of activity, non-phytotoxicity, and long-term persistence of fungitoxicity. [ABSTRACT FROM AUTHOR]

When wheat is stored under suboptimal conditions, a further mycotoxin increase of deoxynivalenol (DON), but especially of mycotoxins produced by storage fungi, e.g. ochratoxin A, is possible, lowering wheat quality and food safety. Different storage trials were conducted under suboptimal storage conditions. Fusarium survival during suboptimal storage was monitored by cultural technique and multiplex-PCR and set into relation to DON contents. Furthermore, XANES spectroscopy was applied on a selected storage trial in order to characterize sulfur speciation in low molecular weight (LMW) subunits of glutenin isolated from suboptimally stored wheat samples highly infected with Fusarium and from wheat infected with Aspergillus and Penicillium. Distinct changes in sulfur speciation were observed in grains infected with storage fungi, especially a significant increase of higher oxidation states (sulfoxide state, sulfonate state). [ABSTRACT FROM AUTHOR]

Soybean seed samples inoculated with spores of Aspergillus ruber were stored for 20 to 140 days at 25 ± 1 °C with moisture content varying from 11.3% to 17.7%. Seeds were colonised by the fungus within 20 days at all moisture levels. Ergosterol concentration in seeds increased with time of storage, being slow in samples with moisture content of 11.3% to 13.1% and more rapidly in those with higher moisture content. Free fatty acid (FFA) content also increased following the pattern of ergosterol. Equations were used to predict minimum safe storage period of the seeds at 25 °C. The rise in FFA is due to increase of A. ruber mass within the seed. Fungal growth did not affect fatty acids profiles or iodine index of the extracted oil. The results suggest revision of the present recommendations regarding seed moisture during storage. The FFA concentration of a seed lot can be used as a sensitive indicator of seed deterioration due to storage fungi. [ABSTRACT FROM AUTHOR]

The removal of many synthetic fungicides from the market has created a demand for new, environmentally safe fungicides. Iturin A, a cyclic lipopeptide produced by Bacillus subtilis, has strong antifungal properties and low mammalian toxicity. To determine the efficacy of this compound as a potential fungicide on stored feed grains, lots of corn, peanuts and cottonseed were treated with varying concentrations of iturin A. The mycoflora of treated seed was assayed along with that of untreated seed and seed treated with fungicides used commercially for planting seed. Fungal species varied considerably in their sensitivity to iturin A. Significant reductions in total mycoflora occurred in most seed lots tested at iturin A concentrations of 50 to 100 ppm. [ABSTRACT FROM AUTHOR]

The changes in moisture content, storage mycoflora and aflatoxin B (AFB) in bran from untreated or raw rice (Rr) and parboiled rice (Pbr) stored in small lots in polyethylene bags were studied at 15-day intervals up to 60 days, using five lots of each type of bran. Deterioration was more rapid with reference to all the three parameters, in Rr bran compared to Pbr bran, the former becoming completely overgrown and caked with fungi by the end of 60 days. Aspergillus flavus was the dominant fungus in Pbr bran, whereas A. candidus and Trichoderma viride were abundant in Rr bran. The frequency of incidence as well as concentration of AFB increased with storage time in both types of bran, but the rate of increase as well as overall concentration were much higher in Rr bran. Thus raw rice bran is unsuitable for prolonged storage. [ABSTRACT FROM AUTHOR]

Thirty four samples of rice bran, of which 9 were from raw (untreated) rice (RR) and 25 from parboiled rice (PbR) were collected from commercial rice mills in and around Madras and analysed for storage mycoflora and mycotoxins. Fungi of the Aspergillus flavus group were present in 29 of the 34 samples (8 from RR and 21 from PbR) in quantities ranging from <1-432 thousand propagules/g, though not always as the dominant mycoflora. Fungal numbers were usually higher in RR than in PbR samples. Five of the 9 RR samples and 6 of the 25 PbR samples were positive for aflatoxins. Among 29 isolates of A. flavus obtained from the bran samples, 16 isolates −6 from RR bran and 10 from PbR bran - were found to be toxigenic in vitro. Some isolates of A. candidus also seemed to produce aflatoxin and other fluorescent substances. [ABSTRACT FROM AUTHOR]

120 species and 38 genera were collected from 64 samples of paddy grains on glucose- and cellulose-Czapek's agar at 28 °C. The total count of glycophilic and cellulose-decomposing fungi fluctuated between 216-29760; and 124-11320 colonies/g paddy grains on the two media, respectively. On glucose agar, the most common species were Aspergillus niger, A. flavus, A. sydowi, A. terreus, A. fumigatus, A. ochraceus, Penicillium chrysogenum, P. corylophilum, Fusarium oxysporum, Alternaria alternata, Cladosporium cladosporioides, Trichoderma viride and Mucor racemosus. On cellulose agar with pH 5.5 & 8.0, the most prevalent fungi were Stachybotrys chartarum, S. bisybi, Aspergillus niger, A. flavus, Fusarium oxysporum, Alternaria alternata, Drechslera sativus and Acremonium strictum. Extracts from 64 paddy samples were tested against brine shrimp larvae ( Artemis salina). Of these 9 displayed varying degrees of toxicity. Trichothecene-toxins were detected in the extracts of three paddy samples only. Diacetoxyscirpenol and T-2 toxin were detected in two samples and only T-2 toxin in the other. [ABSTRACT FROM AUTHOR]