Search

Study was conducted to evaluate the optimal utilization of protected soy bean meal with secondary compound from banana stem juice in ration for sheep and its effect on sheep performance. Soy bean meal was mixed with banana stem juice at ratio 1:1 (w/v) then dried in the oven at temperature 90oC. This protected soy bean meal was used to substitute commercial concentrate in sheep ration. The experiment used 24 head male lamb Sumatera composite breed. The lambs were grouped into six group based on its body weight and was assigned to one of 4 diets treatment. The sheep was fed on grass basal diet and supplemented with commercial concentrate. Data recorded were feed consumption, nutrient digestibility, average daily gain, feed efficiency and nitrogen utilization. Study was conducted in randomized complete block design and data obtained were analyzed using general linier model from SAS program. Results show that dry matter intake (DMI) significantly (P < 0.05) increased with concentrate substitution by protected soy bean meal, however, there was no significant different (P > 0.05) between R10, R20 and R30. The increasing in DMI is followed by the increasing crude protein (CP) from 8.75 (R0) to 10.64; 11.68 and 12.32 g/BB0.75 respectively for R10; R20 and R30. Commercial concentrate substitution by protected soy bean meal significantly increased DM and CP digestibility at all levels. However, this substitution did not significantly affected organic matter (OM), neutral detergent fiber (NDF) and acid detergent fiber (ADF) digestibility. Nitrogen excretion in urine was only increased at this level 30% substitution but nitrogen retention increased at substitution levels 20 and 30%. From this study can be concluded that commercial concentrate substitution with protected soy bean meal in the diet only increased CP consumption and CP digestibility but not average daily gain of lamb.

The study was conducted to evaluate the rumen degradability of soy bean meal when it was mixed with banana stem juice and was subsequently heated. Banana stem juice was used as source of tanin to protect protein in soy bean meal from degradation in the rumen. The banana stem juice (in volume) was mixed with soy bean meal at three ratio, which were 1 : 1, 1:2 and 1 : 3 (w/v). These mixing was then heated at 60oC and 90oC. The heating was done in an oven. Sample from each treatment was then filled into polyester bag with porosity of 45 mm and the size of 5 x 9 cm. The bags were incubated in the rumen of fistulated sheep for 0, 2, 4, 8, 16, 24 and 48 hours. The experiment was carried out in factorial 3 x 2, with three levels of banana stem juice and 2 levels of heating and was arranged in completely randomized design. Untreated soy bean meal was also incubated used as control. Degradation data of dry matter and crude protein at each incubation time was fitted into exponential equation, P = a + b (1 - ect). Results shows that there was a significant interaction factor of stem juice ratio and heating on water soluble substrate (constant A) where heating at 90oC with ratio 1 : 2 (w/v) resulted in the highest a. The insoluble but fermentable substrate (B) was decreased by the increasing concentration of banana stem juice, while the rate of protein degradation of b fraction was lower compared to control. In conclusion, banana stem juice can reduce the rate of degradation of soy bean meal in the rumen.

Protein with low resistant of rumen degradability must be protected, which part of its protein could reach post ruminal and able to supply amino acids for ruminant. The experiment was conducted to obtain the best ratio of soy bean meal with banana stem juice as feed supplement protecting protein. Rumen degradability using rumen fistulated sheep were evaluated in saccous at incubation times of 0, 2, 4, 6, 12, and 24 hours. The best ratio in the in sacco experiment was used for further in vivo experiment. Fifteen growing lambs with average live weight of 18.6 ± 2.2 kg were grouped according to body weight in trial with a simple randomized design. Three treated rations were R0 = control diet with 0% protected soy bean meal, R50 = diet with 50% protected soy bean meal, and R100 = diet with 100% protected soy bean meal. All diets were iso nitrogen and iso energy (CP 18% and TDN 75%). Diet consisted approximately 30% king grass and 70% concentrate. The experimental diets were offered for 12 weeks. The result from in sacco experiment showed that the best ratio between soybean meal and banana stem juice was 2:1 w/v (R2). This mixture had the highest rumen undegradable dry matter and protein. Result from in vivo experiment showed that the intake and digestibility of dry matters, the intake and digestibility of crude protein. The intake and digestibilities of dry matter intakes and digestibilitie of crude protein, pH value, NH3-N, total bacteria, purin and total VFA in rumen fluid were not affected by feed supplement. The same average body weight at the beginning of study resulted same body weight at the end of study. The ADG of R50 and R100 (138.1 and 122.2 g) were respectively not different with the control, RO (120.9g). It was concluded that soy bean meal and banana stem juice 2:1 w/v was the best ratio, but as feed supplement with high resistant rumen degradable protein can not produce higher growth rate than the control group.

El objetivo del estudio fue comparar el método de digestibilidad proteica aparente in vivo en ratas con el método alternativo in vitro con pepsina, así como determinar el grado de correlación entre ambosmétodos. Para ello, se determinó la digestibilidad proteica de tres insumos utilizados en la elaboración de alimentos comerciales para perros (torta de soya, harina de carne y harina de pollo), tres alimentos comerciales para cachorros (C1, C2 yC3); tres alimentos comerciales para perros adultos (A1,A2 yA3); y un control que fue caseinato de sodio. Para la prueba in vivo se utilizaron 60 ratas albinas de laboratorio (Rattus norvegicus) de 23 días de edad ypara la prueba in vitro se utilizó el método de la pepsina estandarizado en el Laboratorio deNutrición de la Facultad deMedicina Veterinaria de la UNMSM. El coeficiente de correlación entre ambos fue de 0.94 y la regresión fue: Y, in vivo = 11.037 + 0.804 (X, in vitro), siendo la digestibilidad proteica in vitro estadísticamentemayor que la digestibilidad aparente in vivo (p< 0.05). Se concluye que existe una alta correlación entre los métodos empleados para medir la digestibilidad proteica de insumos y alimentos usados en la alimentación canina., The aim of this study was to compare the in vivo apparent protein digestibility method in rats and the in vitro pepsin digestibility method as well as their level of correlation. Three protein sources used in commercial dog foods (soybean meal, meat meal, and chicken meal), three commercial adult dog foods (A1, A2, and A3), three commercial puppy foods (C1, C2, and C3), and sodium caseinate as a control groupwere evaluated. For the in vivo assay, 60 twenty-three-day-old albino laboratory rats (Rattus norvegicus) were used and for the in vitromethod the standardized pepsin method used at the Nutrition Laboratory, Faculty of Veterinary Medicine, UNMSM was evaluated. The correlation coefficient between the in vivo and in vitro methods was 0.94, whereas the in vitro digestibility was statistically higher than the in vivo digestibility (p

El objetivo del estudio fue comparar el método de digestibilidad proteica aparente in vivo en ratas con el método alternativo in vitro con pepsina, así como determinar el grado de correlación entre ambos métodos. Para ello, se determinó la digestibilidad proteica de tres insumos utilizados en la elaboración de alimentos comerciales para perros (torta de soya, harina de carne y harina de pollo), tres alimentos comerciales para cachorros (C1, C2 y C3); tres alimentos comerciales para perros adultos (A1, A2 y A3); y un control que fue caseinato de sodio. Para la prueba in vivo se utilizaron 60 ratas albinas de laboratorio (Rattus norvegicus) de 23 días de edad y para la prueba in vitro se utilizó el método de la pepsina estandarizado en el Laboratorio de Nutrición de la Facultad de Medicina Veterinaria de la UNMSM. El coeficiente de correlación entre ambos fue de 0.94 y la regresión fue: Y, in vivo = 11.037 + 0.804 (X, in vitro), siendo la digestibilidad proteica in vitro estadísticamente mayor que la digestibilidad aparente in vivo (p< 0.05). Se concluye que existe una alta correlación entre los métodos empleados para medir la digestibilidad proteica de insumos y alimentos usados en la alimentación canina. The aim of this study was to compare the in vivo apparent protein digestibility method in rats and the in vitro pepsin digestibility method as well as their level of correlation. Three protein sources used in commercial dog foods (soybean meal, meat meal, and chicken meal), three commercial adult dog foods (A1, A2, and A3), three commercial puppy foods (C1, C2, and C3), and sodium caseinate as a control group were evaluated. For the in vivo assay, 60 twenty-three-day-old albino laboratory rats (Rattus norvegicus) were used and for the in vitro method the standardized pepsin method used at the Nutrition Laboratory, Faculty of Veterinary Medicine, UNMSM was evaluated. The correlation coefficient between the in vivo and in vitro methods was 0.94, whereas the in vitro digestibility was statistically higher than the in vivo digestibility (p