5 results on '"somah"'
Search Results
2. Wasta and Favoritism: The Case of Kuwait
- Author
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Alenezi, Marim, Hassan, Samia, Abdelrahim, Yousif, Albadry, Omaima, Kacprzyk, Janusz, Series Editor, Gomide, Fernando, Advisory Editor, Kaynak, Okyay, Advisory Editor, Liu, Derong, Advisory Editor, Pedrycz, Witold, Advisory Editor, Polycarpou, Marios M., Advisory Editor, Rudas, Imre J., Advisory Editor, Wang, Jun, Advisory Editor, Alareeni, Bahaaeddin, editor, and Hamdan, Allam, editor
- Published
- 2023
- Full Text
- View/download PDF
3. EFFICACY ASSESSMENT OF CRYO STORAGE OF DONOR HEARTS BY IMAGEJ BASED IMAGE ANALYSIS
- Author
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Bao-quan Lin, Sheng-sheng Yang, Zhi-yong Zeng, and Cong-wen Zhuang
- Subjects
orthotopic cardiac transplantation ,university of wisconsin solution ,celsior ,somah ,cardiac storage solution ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Aim. Donor organ injury during cold preservation before transplantation negatively impacts graft survival. The current study was to examine available evidences for the efficacy of different cold storage solutions that are used to preserve donor hearts in vitro prior to orthotopic transplantation.Material and methods. A systematic search of full-length articles published from 1980 to August 2012 was performed in PubMed and Google Scholar. Detailed searches were also made for availability of any sourceware for histopathology images of endomyocardial biopsies of stored hearts.Results. Not even a single controlled trial has been published relating to this topic. However, we assessed all available literature pertaining to this topic, and performed original, simple yet innovative analyses using ImageJ, a Java based image analyses program, to show the tremendous power to objectively examine the efficacy of the storage solution. Our analysis suggest that ImageJ may be conveniently used to obtain evidences (or lack of it) of ischemic injury of donor hearts during cold storage.Conclusions. Even the UNOS database does not provide histopathological evidences of cardiac biopsies of orthotopically transplanted hearts. We, however, make the case of the need for image analyses and making availability of images to allow establishing evidence of the usefulness of these storage solutions. We recommend obtaining endomyocardial biopsy prior to orthotopic transplantation and create a registry of H&E stained slides. This is the only step that will direct us towards evidence based care of such highly critical patients who need the equally challenging surgical intervention of cardiac transplantation.
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- 2014
- Full Text
- View/download PDF
4. Novel vs clinical organ preservation solutions: improved cardiac mitochondrial protection.
- Author
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Ferng, Alice S., Schipper, David, Connell, Alana M., Marsh, Katherine M., Knapp, Shannon, and Khalpey, Zain
- Subjects
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HEART transplantation , *HEART failure , *CORONARY disease , *OXYGEN consumption , *CELLULAR bioenergetics - Abstract
Background: Heart transplantation remains the gold standard for end-stage heart failure, with current ex vivo organ storage times limited to 4 to 6 h before critical tissue damage occurs. Many preservation solutions exist in an attempt to limit both ischemic and reperfusion damage. In order to compare the effects of various storage solutions, mitochondrial function can be used to provide a sensitive analysis of cellular metabolic function.Methods: Experimental plates were seeded with cardiac myoblasts and kept in suspended animation for either 4 or 8 h at either 4o or 21 °C, in Celsior®, Perfadex®, or Somah storage solutions. Cells were then reanimated for 1 h at 37 °C to simulate a reperfusion or clinical transplant scenario. Cellular bioenergetics were measured immediately thereafter to examine biochemical differences between preservation solutions and their effectiveness on preserving metabolic function.Results: The oxygen consumption rates of Somah solution were significantly higher than Celsior® and Perfadex® at 4 °C, with the exception of Perfadex® at 4o for 4 h. This effect was sustained up to 8 h. At 21 °C, oxygen consumption rates of Somah solution are significantly higher than Celsior® and Perfadex® at basal conditions after 4 h, but this effect is not sustained after 8 h.Conclusions: The purpose of this experiment was to study the efficacy of various preservation solutions on a mitochondrial level. The significantly higher oxygen consumption rates of Somah at 4 °C suggests that Somah solution may have the ability to protect cellular mitochondrial integrity, improve transplanted organ function by reducing ischemic-reperfusion injury, and thereby improve transplant outcomes. Given that Somah offers benefits over Celsior® and Perfadex® at 4 °C, it should be a target in future organ preservation solution research. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
5. Novel vs clinical organ preservation solutions: improved cardiac mitochondrial protection
- Author
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Katherine M. Marsh, David Schipper, Zain Khalpey, Alice S. Ferng, Alana M. Connell, and Shannon M. Knapp
- Subjects
Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Pathology ,Cardiac myoblasts ,Bioenergetics ,medicine.medical_treatment ,Organ Preservation Solutions ,Transplants ,030204 cardiovascular system & hematology ,030230 surgery ,Mitochondrion ,Mitochondria, Heart ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Somah ,Perfadex ,Heart transplantation ,Metabolic function ,business.industry ,Organ preservation solution ,Heart ,General Medicine ,Organ Preservation ,medicine.disease ,Celsior ,Mitochondria ,Heart failure ,Cardiology ,Surgery ,Cardiology and Cardiovascular Medicine ,business ,Ex vivo ,Research Article - Abstract
Background Heart transplantation remains the gold standard for end-stage heart failure, with current ex vivo organ storage times limited to 4 to 6 h before critical tissue damage occurs. Many preservation solutions exist in an attempt to limit both ischemic and reperfusion damage. In order to compare the effects of various storage solutions, mitochondrial function can be used to provide a sensitive analysis of cellular metabolic function. Methods Experimental plates were seeded with cardiac myoblasts and kept in suspended animation for either 4 or 8 h at either 4o or 21 °C, in Celsior®, Perfadex®, or Somah storage solutions. Cells were then reanimated for 1 h at 37 °C to simulate a reperfusion or clinical transplant scenario. Cellular bioenergetics were measured immediately thereafter to examine biochemical differences between preservation solutions and their effectiveness on preserving metabolic function. Results The oxygen consumption rates of Somah solution were significantly higher than Celsior® and Perfadex® at 4 °C, with the exception of Perfadex® at 4o for 4 h. This effect was sustained up to 8 h. At 21 °C, oxygen consumption rates of Somah solution are significantly higher than Celsior® and Perfadex® at basal conditions after 4 h, but this effect is not sustained after 8 h. Conclusions The purpose of this experiment was to study the efficacy of various preservation solutions on a mitochondrial level. The significantly higher oxygen consumption rates of Somah at 4 °C suggests that Somah solution may have the ability to protect cellular mitochondrial integrity, improve transplanted organ function by reducing ischemic-reperfusion injury, and thereby improve transplant outcomes. Given that Somah offers benefits over Celsior® and Perfadex® at 4 °C, it should be a target in future organ preservation solution research. Electronic supplementary material The online version of this article (doi:10.1186/s13019-017-0564-x) contains supplementary material, which is available to authorized users.
- Published
- 2017
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