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Background: Ejaculatory abstinence (EA) duration influences semen parameters. However, the impact of penultimate ejaculatory abstinence (PEA) on conventional and functional sperm parameters remains underexplored. Method: A cross-sectional study recruited 1,503 men from a reproductive center between November 2023 and July 2024. Each participant underwent a physical examination, completed clinical questionnaires, and provided a semen sample for analysis. Generalized linear models were adjusted for potential confounders such as EA to investigate the association between PEA and various sperm parameters. Logistic regression was used to evaluate the relationship between PEA and the risk of high sperm DNA fragmentation index (DFI), oligozoospermia, asthenozoospermia, and necrozoospermia. Results: Participants were categorized into four quartiles based on PEA duration. (Q1: 1-3 days; Q2: 4-5 days; Q3: 6-9 days; Q4: > 9 days). After adjusting for potential confounders, a significant positive linear association was found between PEA and DFI, while a significant negative linear association was observed with progressive sperm motility. The longest PEA duration (Q4) correlated positively with semen concentration (P = 0.025), total sperm count (P < 0.001), and sperm vitality (P < 0.001). Compared to Q1, a PEA of > 9 days (Q4) was associated with higher risks of sperm DFI > 30% (adjusted odds ratio [OR] = 4.25; 95% confidence interval [CI]: 2.37-7.62), asthenozoospermia (adjusted OR = 1.45; 95% CI: 1.07-1.96), and necrozoospermia (adjustedOR = 1.99; 95% CI: 1.07-3.69). Moreover, the risk of sperm DFI > 15% was higher in Q2, Q3, and Q4 compared to Q1. Conclusion: Prolonged PEA adversely affects sperm DFI, progressive motility, and sperm vitality, increasing the likelihood of asthenozoospermia, necrozoospermia, and elevated DFI levels. These findings suggest that both EA and PEA should be considered in fertility assessments, with shorter PEA durations potentially yielding higher quality sperm, thereby enhancing male fertility evaluation and outcomes. [ABSTRACT FROM AUTHOR]

Purpose: Chromosomal abnormalities play an important role in male infertility, which is becoming a significant issue in human fertility. Aim of this study was to evaluate the incidence of spermatic aneuploidies and diploidies in human sperm, according to semen parameters. Methods: We performed semen analysis according to the 6th edition of WHO criteria in 50 male subjects; samples were divided into normozoospermic (n = 23) or those with altered seminal parameters (n = 27). To assess chromosomal numerical alterations of sperm, fluorescence in situ hybridization (FISH) was used. Result: A significant increase in aneuploidies and diploidies was observed in samples with altered seminal parameters. Furthermore, stratifying this group, we observed a significant increase in aneuploidies and total abnormalities in oligozoospermic, asthenoteratozoospermic (AT), and oligoteratoasthenozoospermic (OAT) samples compared to normozoospermic. Conclusion: Our results showed the correlation between altered seminal parameters and numerical chromosomal abnormalities, confirming that sperm FISH analysis could be an additional clinical tool to assess reproductive potential in infertile males. Moreover, our results point to the importance of updating the normality ranges for detecting chromosomal aneuploidies using FISH. [ABSTRACT FROM AUTHOR]

Varicocele (VC) is widely recognized as a prevalent etiological factor contributing to male infertility. It has been established that the generation of reactive oxygen species (ROS) plays a significant role in the progression and development of VC. Antioxidants may regulate ROS levels in these patients. Astaxanthin (ASX) is a carotenoid compound with notable antioxidant and anti‐inflammatory characteristics. The current study postulated that the administration of ASX following varicocelectomy (VCT) could potentially enhance antioxidant status and semen quality in these patients. A total of 40 infertile males with clinical VC and abnormal semen analyses were randomly assigned to take part in the current trial. For 3 months following surgery, the intervention group took ASX (6 mg/day) while the control group received a placebo. After intervention, semen parameters, antioxidant status, and pro‐inflammatory cytokines were compared between the two groups. Regarding semen parameters, antioxidant treatment led to a significant improvement in total and progressive motility in the treatment group (p < 0.05). Additionally, ASX led to a considerable increase in the expression levels of NRF2, Keap1, SOD2, SOD3, and BCL2, though the enhancement in the expression level of SOD3 was not statistically significant (p >.05). However, ASX significantly decreased the BAX expression level (p <.05). Even though the level of total antioxidant capacity (TAC) of seminal fluid (SF) increased significantly in the treatment group (p <.05), the level of total oxidative stress (TOS) in SF did not differ substantially between treatment and control groups (p >.05). Based on inflammatory factors in SF, ASX led to a considerable reduction in levels of TNF‐α, IL‐1β, and IL‐6 (p <.05). Our findings demonstrated that ASX treatment provides an important contribution to VCT outcomes by modulating antioxidant status and pro‐inflammatory cytokines. Our results indicated that ASX may be beneficial as an adjuvant therapy for infertile men following VCT. [ABSTRACT FROM AUTHOR]

There has been much controversy about the true value of varicocele repair for improving fertility and the chances of natural conception. This review summarizes the latest research findings, current professional societies' recommendations, and the conclusions of recent systematic reviews and meta-analyses. The current evidence suggests a positive role for varicocele repair in improving basic sperm parameters and increasing the likelihood of natural pregnancy. [ABSTRACT FROM AUTHOR]

Background: Varicocelectomy was considered to be beneficial to patients with varicocele-related infertility. However, there are only a few researchers who have explored the relationship between better timing and postoperative semen improvement in patients. Methods: We conducted this meta-analysis by enrolling published prospective studies to find out the best waiting time after varicocelectomy to wait for better improvement of semen quality. An extensive search was conducted in PubMed, Web of Science, and Cochrane Library to identify eligible studies. The included studies were then analyzed comprehensively using STATA software and standardized mean differences (SMDs) and their corresponding 95% confidence intervals were calculated. Results: Our comprehensive analysis showed that after varicocelectomy, follow-up results within 3 months or longer showed a significant improvement in semen parameters compared to the preoperative period. Notably, no further improvement in semen parameters was observed when the follow-up period reached six months or longer (semen volume: WMD: − 0.07 (− 0.29, 0.16); sperm concentration: WMD: − 1.33 (− 2.33, − 4.99); sperm motility: WMD: 2.31 (− 0.55, 5.18); sperm morphology: WMD: 1.29 (− 0.66, 3.24); sperm total motile count: WMD: 3.95 (− 6.28, 14.19)). Conclusions: Three months after varicocelectomy may be the optimal time for semen parameters compared to six months or even longer, which means it is also the preferable time for conception. However, more well-designed prospective studies are needed in the future to validate our conclusion. [ABSTRACT FROM AUTHOR]

Male infertility along with altered semen parameters have been related to smoking. Smoking-related elevations in serum and seminal lead (Pb) and arsenic (As) may play a role in mediating the toxic effects of smoking on seminogram. This research aims to determine whether smoking has any significant impact on Pb and As levels in the seminal plasma and serum, as well as on the various semen parameters, when compared to nonsmokers. In total, 80 adult males were included: 60 smokers and 20 age-matched nonsmokers. Based on the number of cigarettes smoked/day (CPD), the smokers were categorized into mild (1–10), moderate (11–20), and severe (> 20). The analysis of semen was conducted in accordance with the 2010 WHO laboratory manual. Using an atomic absorption spectrophotometer, Pb and As concentrations in the serum and seminal plasma of all groups were determined. Compared to nonsmokers, smokers had a significantly reduced sperm count, motility, and viability, as well as a larger percentage of aberrant forms (P = 0.001, 0.025, 0.034, 0.002 respectively). Smokers had higher Pb concentrations in their serum and seminal fluid than nonsmokers (P = 0.002, 0.001 respectively). Seminal Pb had a significant negative correlation with sperm count (P = 0.004, r = -0.320). Serum Pb levels were found to positively correlate with seminal Pb levels (P 0.001, r = 0.648), and cigarette smokers had substantially greater seminal As levels than nonsmokers (P = 0.024). Sperm viability was strongly inversely related to seminal As (P = 0.042, r = -0.264). Seminal As levels and aberrant sperm shapes were found to be significantly correlated (P = 0.001, r = 0.414). In smokers, a significant positive relationship between seminal As and seminal Pb was observed. Therefore, semen parameters could be adversely affected by smoking through high levels of Pb and As (P = 0.012, r = 0.298). [ABSTRACT FROM AUTHOR]

Cryptorchidism is a common congenital abnormality encountered in dogs. The commonly used treatment for this condition is orchiectomy or orchidectomy. Surgical placement and fixation of the cryptorchid testicle into the scrotum, referred to as orchiopexy, is used in humans. The treatment of cryptorchidism by orchiopexy in dogs is a controversial topic due to the hereditary nature of cryptorchidism in dogs. The present study was focusing for the first time on long-term monitoring of the testicular soundness and function following laparoscopic orchidopexy in young dogs. Seventeen (n.= 17) German shepherd young dogs (180±14 days) were included in the study. All cases enrolled in this study underwent laparoscopic testicular descent and orchidopexy. Ten days postoperatively, the dogs were evaluated by Doppler ultrasound to assess the testicular blood flow. At age of 18-20 months, semen parameters were evaluated in all treated dogs. Clinically, the abdominal wounds were healed 3-4 days after surgery with complete absence of all signs of infection, pain or discomfort. Testicular volume (TV; cm3) and thickness of the mediastinum line (MT; mm) were within normal values (8.65±0.25 cm3 and 1.11±0.85 mm), with normal testicular blood flow. The mean values of semen parameters were (82.68% for individual motility and 90.35% for the percentage of live sperm). In conclusion, laparoscopic descent and orchidopexy can be utilized with keeping the normal testicular functions when performed at young, aged dogs (180±14 days). [ABSTRACT FROM AUTHOR]

Introduction The research regarding the effect of hookah smoking on health is still deficient, even though it has been proven to jeopardize human health by raising the hazard of different types of cancers, infections, and cardiovascular disease. We aimed to study the effect of dual tobacco smoking (hookah and cigarettes) on semen parameters of infertile men. Methods In this cross-sectional study, we studied the effect of different types of smoking patterns on human semen parameters among men who visited IVF laboratories to do a seminal fluid analysis (SFA). A total number of 761 participants were included, divided into the following: 108 dual smokers, 219 hookah smokers, 222 cigarette smokers, and 212 non-smokers. To analyze the effect of dual smoking on normal morphology, an interaction term between the cigarette index and hookah index was used. Results Multivariable regression analysis after adjustment for age, BMI, education level, children, chronic diseases, varicocele, testicular surgery history, infertility duration, and cause revealed no significant difference in the sperm concentration and the percentage of progressive motility between non-smokers, cigarette smokers, or hookah smokers. However, there was a significant difference in the log of normal morphology percentage between the three groups. Cigarette and hookah smoking were significantly associated with having lower percentages of normal morphology. There was a significant difference in the log-normal morphology %, where light and heavy dual smokers had the least exponential beta of log-normal morphology %, 0.43 (95% CI: 0.33–0.55) and 0.36 (95% CI: 0.24–0.53), respectively. Conclusions Dual tobacco smoking can adversely affect sperm morphology.

Abstract Objective Evaluate the impact of SARS-CoV-2 on male fertility. Design Comprehensive analysis of studies exploring virus presence, inflammation, and altered semen parameters. Result(s) While some studies report absence of SARS-CoV-2 in semen, others highlight testicular injury, inflammation, and potential viral orchitis. Scrotal discomfort and altered sperm parameters indicate reproductive implications. Conclusion SARS-CoV-2 may pose significant challenges to male fertility, necessitating further research for a comprehensive understanding of its long-term effects. Limitations include varied testing methods and sample sizes, emphasizing the need for confirmation and detailed post-COVID examinations.

Background: Men's tobacco usage may be a contributing factor in up to 12% of cases of infertility in couples due to decreased semen parameters. It is the reality that Sudan is home to the greatest number of nicotine snuffers (toombaks) worldwide. Unlike snuff, the detrimental effects of cigarette smoking on sperm parameters are well-documented, even though they apply to other smokeless nicotine products. Aim: to research the influence on semen variables related to toombak snuffing. Methods: A comparative study analysed 120 subjects, out of which 50 were toombak snuffers (cases), (mean age = 33.9±6.4 years), and 70 were non-snuffers (control), (mean age= 33.5±6.9 years) attended Hawa Fertility Centre in the period from November 2021 to November 2022. Data regarding demographics, duration of tobacco snuffing, frequency of snuffing per day, diagnosis, and seminal analysis parameters were compared between groups. Results: Among patients in the snuffer group, the majority of them had snuffing duration from 10-20 years (n = 20; 40%) and had snuffing frequency >20 times per day (n = 27; 54%). Compared to the control group, snuffing was a significant predictor of low count. (oligiospermia and azoospermia) (OR = 3.8; 95% CI: 1.6-9.1; P = 0.002), low motility <42% (OR = 3.6; 95% CI: 1.7-7.9; P = 0.001), low progressive motility <30% (OR = 2.0; 95%CI: 1.3-4.2; P = 0.018) and normal morphs <4% (OR = 2.7; 95%CI: 1.2-5.7; P = 0.009). The snuffing duration above 20 years was a significant risk factor for a low count. (oligiospermia and azoospermia) (OR = 16.8; 95% CI: 2.6-46.3; P = 0.003), low motility <42% (OR = 11.0; 95% CI: 2.0-60.0; P = 0.006), low progressive motility <30% (OR = 10.8; 95%CI: 1.9-59.8; P = 0.007) and normal morphs <4% (OR = 10.6; 95%CI: 2.1-60.0; P= 0.007). The snuffing frequency above 20 times per day was a significant risk factor for low count (oligiospermia and azoospermia) (OR = 7.9; 95% CI: 1.8-34.5; P = 0.008), low motility <42% (OR = 3.4; 95% CI: 1.7-12.1; P = 0.041), low progressive motility <30% (OR = 4.1; 95% CI: 1.1-14.9; P = 0.033), but not normal morphs <4% (P = 0.083). Conclusion: Toombak snuffing had a major detrimental impact on spermatogenesis, which in turn affected sperm motility, measure, and shape. Furthermore, longer duration (>20 years) and intensive toombak snuffing use (>20 times per day) were significantly correlated with low motility, shape, and quantity of sperm. [ABSTRACT FROM AUTHOR]

Introduction: The research regarding the effect of hookah smoking on health is still deficient, even though it has been proven to jeopardize human health by raising the hazard of different types of cancers, infections, and cardiovascular disease. We aimed to study the effect of dual tobacco smoking (hookah and cigarettes) on semen parameters of infertile men. Methods: In this cross-sectional study, we studied the effect of different types of smoking patterns on human semen parameters among men who visited IVF laboratories to do a seminal fluid analysis (SFA). A total number of 761 participants were included, divided into the following: 108 dual smokers, 219 hookah smokers, 222 cigarette smokers, and 212 non-smokers. To analyze the effect of dual smoking on normal morphology, an interaction term between the cigarette index and hookah index was used. Results: Multivariable regression analysis after adjustment for age, BMI, education level, children, chronic diseases, varicocele, testicular surgery history, infertility duration, and cause revealed no significant difference in the sperm concentration and the percentage of progressive motility between non-smokers, cigarette smokers, or hookah smokers. However, there was a significant difference in the log of normal morphology percentage between the three groups. Cigarette and hookah smoking were significantly associated with having lower percentages of normal morphology. There was a significant difference in the log-normal morphology %, where light and heavy dual smokers had the least exponential beta of log-normal morphology %, 0.43 (95% CI: 0.33–0.55) and 0.36 (95% CI: 0.24–0.53), respectively. Conclusions: Dual tobacco smoking can adversely affect sperm morphology. [ABSTRACT FROM AUTHOR]

Objective: Evaluate the impact of SARS-CoV-2 on male fertility. Design: Comprehensive analysis of studies exploring virus presence, inflammation, and altered semen parameters. Result(s): While some studies report absence of SARS-CoV-2 in semen, others highlight testicular injury, inflammation, and potential viral orchitis. Scrotal discomfort and altered sperm parameters indicate reproductive implications. Conclusion: SARS-CoV-2 may pose significant challenges to male fertility, necessitating further research for a comprehensive understanding of its long-term effects. Limitations include varied testing methods and sample sizes, emphasizing the need for confirmation and detailed post-COVID examinations. [ABSTRACT FROM AUTHOR]

Background & Objective: The viruses are considered as one of the most prevalent factors that cause infertility because they represent a significant role in sexual transmission disease. The purpose of this study was to evaluate the correlations between viruses (HSV) viral load and semen parameters in groups of fertile and infertile Iraqi men. Materials & Methods: This study comprised fifty infertile males and fifty fertile healthy males. Seminal fluid analysis was conducted on all participants to detect the presence of HSV using the HSV1, 2 quantification kits. Results: There was a high significant correlation between sperm concentration and percentage of rapid linear progression motility (r= 0.478**) of infertile men. A strong meaningful connection was seen between the percentage of sperm concentration and both the percentage of sluggish linear or nonlinear motility (r = 0.681**) and the percentage of non-progressive motility (r = 0.604**). There was a strong negative connection (r = -0.725**) between the concentration of sperm and the number of immotile spermatozoa. On the others hand, there was a high significant correlation between rapid linear progression and slow linear or nonlinear (r =0 .413**) non-progressive motility (r = 0.704**) respectively, and high negative significant between rapid linear progression and immotile spermatozoa. The correlation between slow linear or nonlinear and non-progressive motility was highly significant (r= 0.555**) and negative significant between slow linear or nonlinear and immotile spermatozoa. Finally, A strong negative correlation (-0.813**) was observed between non-progressive motility and immotile spermatozoa. Conclusion: The study found that HSV infection had an effect on semen parameters and will cause male infertility. [ABSTRACT FROM AUTHOR]

OBJECTIVE: The aim of this study is to evaluate the relationship between semen parameters, complete blood count, and hormone levels on the day of spermiogram. METHODS: Semen parameters of 230 patients who were examined for full blood count test and hormone levels on the day of spermiogram were included in the study. Patients were grouped according to the total motile sperm count (TMSC), semen parameters, hemogram, and hormone levels were compared between groups. RESULTS: No statistically significant difference was found between groups in neutrophil ratios, neutrophil, lymphocyte, platelet counts, neutrophile-to-lymphocyte ratio (N/L), and platelet-to-lymphocyte ratio (P/L). However, white blood cell (WBC) and lymphocyte counts were weakly positively correlated with sperm concentration (p=0.021, p=0.026), and a weakly significant positive correlation was found with WBC and neutrophil count for motility (p=0.038, p=0.004). FSH level was found to be lower in cases with TMSC >20 m than those with TMSC <5 m and 5-10 m (p=0.004, p=0.022). LH was found to be lower in cases with TMSC >20 m than those with TMSC <5 m (p=0.048). A negative correlation was found for both FSH and LH levels with sperm concentration, motility, and TMSC (p<0.001, p=0.014). CONCLUSION: In this study, a significant negative correlation was demonstrated between FSH, LH levels and sperm concentration, motility, TMSC. N/L and P/L cannot be used as predictive markers of sperm quality. The results of a significant positive correlation between WBC, neutrophil counts, and sperm parameters encourage researchers to conduct prospective randomized controlled trials with larger sample sizes and different inflammatory and hormonal markers. [ABSTRACT FROM AUTHOR]

IntroductionUreaplasma urealyticum (UU) is a common pathogen associated with genital tract infections in infertile males. However, its impact on semen quality, embryo development, and in vitro fertilization (IVF) outcomes remains underexplored. This study aims to evaluate the effect of male UU infection on semen parameters, embryo development, pregnancy outcomes, and neonatal health in infertile couples.MethodsA retrospective analysis was conducted on 1,215 infertile couples at the First Hospital of USTC. Participants were divided into two groups based on the male partner’s UU infection status: UU-positive (n=249) and UU-negative (n=966). Semen parameters (sperm concentration, motility, morphology, anti-sperm antibodies, DNA stainability) were assessed. Embryo development was evaluated through fertilization rates and blastocyst formation rates. Pregnancy outcomes (clinical pregnancy, live birth rates, miscarriage rate) and neonatal health (gestational age, birth weight, Apgar scores, preterm delivery) were also compared.ResultsSemen parameters, including sperm concentration, motility, and morphology, were similar between the UU-positive and UU-negative groups. However, the UU-positive group had significantly higher levels of anti-sperm antibodies (ASA) (p=0.020) and higher DNA stainability (HDS) (p=0.014). Despite these differences, embryo quality, as measured by fertilization rates and blastocyst formation rates, was not significantly different between the two groups. Pregnancy outcomes, including clinical pregnancy and live birth rates, were also comparable. While the UU-positive group had a slightly higher miscarriage rate, this difference was not statistically significant. Neonatal outcomes, including gestational age, birth weight, Apgar scores, and preterm delivery rate, did not differ significantly between the two groups.DiscussionThe study suggests that while male UU infection may adversely affect certain semen parameters, its impact on IVF outcomes—such as embryo quality, pregnancy rates, and neonatal health—appears to be minimal. These findings support the continued use of IVF as a viable and safe option for infertile couples with male UU infection, as it does not significantly influence reproductive or neonatal outcomes.

Objectives: The current study assessed the epidemiological trends of semen phenotypes and their association with ethnicity among men seeking fertility treatment in the United Arab Emirates (UAE). Methods: This retrospective study assessed the anthropometric information including age, body mass index (BMI), and nationality, along with semen parameters of men who visited a Fertility Center in Abu Dhabi, UAE between January 2011 and July 2022. To understand the epidemiological trend of semen parameters amongst UAE nationals, propensity score analysis and logistic regression were performed. Thus, the exposure variable of interest is ethnicity, categorized into UAE nationals (Emirati) and Others (minus UAE; Global). Statistical analysis was performed using SPSS, R packages and STATA. Results: In this study, 32,664 samples were collected from 19,482 patients from 113 countries worldwide over a period of 11 years. Most participants made multiple visits, with around 40 % attending at least once. Following covariates adjustment, logistic regression indicated a non-significant increase (4 %) in the prevalence of asthenozoospermia among the UAE population compared to Global. Further modeling adjusted for propensity score and Emirati status suggested that Emiratis were 13 % less likely to have lower total sperm count (TSC) compared to Global (p

Objective: This study focuses on the association between seminal concentration of prosaposin and ambient air pollutants and whether the association affects the normal fertilization rate in vitro fertilization (IVF) treatment. Methods: The cohort of 323 couple participants aged 22–46 was recruited from Jan. 2013 to Jun. 2018. At enrollment, resident address information was obtained and semen parameters of male counterparts were evaluated according to WHO criteria. We used inverse distance weighting interpolation to estimate the levels of ambient pollutants (SO2, O3, CO, NO2, PM2.5, and PM10) in the surrounding area. The exposure of each participant was estimated based on the data gathered from air quality monitoring stations and their home address over various periods (0–9, 10–14, and 0–90 days) before semen sampling. The generalized linear regression model (GLM) and the Bayesian kernel machine regression (BKMR) were used to analyze the associations between pollutants, semen parameters, prosaposin, and normal fertilization. Additionally, the mediating effect of prosaposin and semen parameters on the link between pollutants and normal fertilization was investigated. Results: GLM and BKMR showed exposure to ambient air pollutants was all associated with the concentration of seminal prosaposin, among them, O3 and CO were also associated with normal fertilization (-0.10, 95 %CI: −0.13, −0.06; −26.43, 95 %CI: −33.79, −19.07). Among the semen parameters, only the concentration of prosaposin and total motile sperm count (TMC) was associated with normal fertilization (0.059, 95 %CI: 0.047, 0.071; 0.016, 95 %CI: 0.012, 0.020). Mediation analysis showed that prosaposin played a stronger mediating role than TMC in the relationship between short-term exposure to O3 and fertilization (66.83 %, P0.05). Conclusion: Seminal plasma prosaposin showed a stronger meditating effect reflect the correlation between ambient air pollutants and normal fertilization rate than conventional semen parameters, which may be used as one of the indicators between pollution and fertilization in IVF.

This study was aimed to elucidate the relationship between sperm DNA fragmentation index (DFI) and semen parameters, and to investigate the impact of these parameters on in vitro fertilization-embryo transfer (IVF-ET) outcomes. The study was conducted on 159 couples undergoing IVF-ET treatment at the Department of Reproductive Health from January 2019 to October 2023. The case group was comprised of 79 patients with sperm DFI of ≥15%, and the control group had 80 patients with 0.05). However, marked differences were found in the rates of sperm progressive motility, total sperm motility, normal morphology, high-quality embryos, and transferable embryos (p < 0.05). The correlation analysis between sperm DFI and semen parameters exhibited positive correlation between sperm DFI and total sperm count (p < 0.05). The negative correlations were found between the sperm DFI and sperm progressive motility, total sperm motility, or normal morphology (p < 0.01). The findings demonstrated that incorporating sperm DFI as a standard component of semen analysis was advisable, and the sperm DFI as reference tool assisted in predicting the early embryonic development in IVF-ET patients.

Abstract To figure out how does SARS-CoV-2 affect sperm parameters and what influencing factors affect the recovery of sperm quality after infection? We conducted a prospective cohort study and initially included 122 men with SARS-CoV-2 infection. The longest time to track semen quality after infection is 112 days and 58 eligible patients were included in our study eventually. We subsequently exploited a linear mixed-effects model to statistically analyze their semen parameters at different time points before and after SARS-CoV-2 infection. Semen parameters were significantly reduced after SARS-CoV-2 infection, including total sperm count (211 [147; 347] to 167 [65.0; 258], P

Introduction. Today, infertility is a global problem that affects about 48.5 million married couples worldwide. It has been suggested that epigenetic aberrations are of great importance for reproductive health, as they account for an interactive relationship between genomic landscape, interplay with gene environment and disease phenotype. A new understanding on etiology of complex non-Mendelian disease traits has aroused a growing interest in reproductive epigenetics.Aim: to analyze available publications on epigenetic aspects of male and female infertility as well as nutrition-related risk factors.Materials and Methods. There was conducted a search for publications in the electronic databases PubMed, Google Scholar and Library to be selected in accordance with PRISMA recommendations. All relevant articles published before November 2023 were included in this review. As a result of the search, there were extracted 530 publications from PubMed, 57 publications – from eLibrary and 23 publications – from Google Scholar. Duplicates and non-full-text article versions were excluded.Results. Environmental factors play an important role in generation and maintenance of epigenetic marks. DNA methylation abnormalities can lower human fertility. Altered protamine level may affect epigenetic paternally transmitted DNA information. Long-term infertility is associated with a modified methylome in euploid blastocysts primarily affecting regulation of genomic imprinting. Both excess and deficiency of trace elements are associated with adverse pregnancy outcomes, similarly applied infertility.Conclusion. Despite that epigenetic mechanisms, genes, nutrition and dietary supplements discussed here affect infertility, while a relevant recommended dose has not yet been determined, it was noted that such parameters may positively influence fertility. However, more comprehensive and longitudinal human studies are required to examine their relationship to male and female reproductive functions.

Abstract Many viruses infect the male genital tract with harmful consequences at individual and population levels. In fact, viral infections may induce damage to different organs of the male genital tract (MGT), therefore compromising male fertility. The oxidative stress, induced during viral-mediated local and systemic inflammation, is responsible for testicular damage, compromising germinal and endocrine cell functions. A reduction in sperm count, motility, number of normal sperm and an increase in DNA fragmentation are all common findings in the course of viral infections that, however, generally regress after infection clearance. In some cases, however, viral shedding persists for a long time leading to unexpected sexual transmission, even after the disappearance of the viral load from the blood. The recent outbreak of Zika and Ebola Virus evidenced how the MGT could represent a reservoir of dangerous emergent viruses and how new modalities of surveillance of survivors are strongly needed to limit viral transmission among the general population. Here we reviewed the evidence concerning the presence of relevant viruses, including emergent and re-emergent, on the male genital tract, their route of entry, their adverse effects on male fertility and the pattern of viral shedding in the semen. We also described laboratory strategies to reduce the risk of horizontal or vertical cross-infection in serodiscordant couples undergoing assisted reproductive technologies.

Purpose: This study aimed to investigate the effects of ejaculatory abstinence on sperm parameters. Methods: This analysis was registered in PROSPERO (CRD42023472124). We performed a search on PubMed using the following text terms: (("sperm parameters" OR "sperm analysis" [Mesh]) AND ("sperm DNA fragmentation" OR "DNA fragmentation" [Mesh]) AND ("sexual abstinence" [Mesh] OR "abstinence")) and an advanced search in Scopus using the terms ("sperm parameters" OR "sperm parameters" OR "DNA fragmentation") AND ("abstinence"). The sperm parameters that were investigated were sperm volume, total sperm motility, progressive sperm motility, sperm concentration, sperm morphology, and sperm DNA fragmentation (SDF). A two-day cut-off as a "short" or "long" abstinence period has been defined. Results: Thirteen studies published between 2013 and 2022 were included in this meta-analysis. A total of 2,315 patients, ranging from 6 to 836 from each cohort, were enrolled in the study. We showed that longer abstinence time was associated with greater sperm concentration (mean difference [MD]: 8.19; p <0.01), sperm volume (MD: 0.96; p <0.01), and higher SDF (MD: 3.46; p <0.01), but lower progressive sperm motility (MD: -1.83; p <0.01). Otherwise, no statistically significant difference was observed in patients with longer vs. shorter abstinence times regarding total sperm motility (MD: -1.83; p = 0.06). Meta-regression analysis showed that days of abstinence were positively and linearly related to sperm concentration (slope: 3.74; p <0.01) and SDF (slope: 0.65; p = 0.044). Conclusions: According to our data, short ejaculatory abstinence is associated with better sperm quality. Indeed, a higher percentage of progressive sperm motility and lower levels of SDF have been reported in a short abstinence cohort. In contrast, the long abstinence group reported a higher sperm concentration. [ABSTRACT FROM AUTHOR]

This study investigated the association of blood and semen Bisphenol A (BPA) levels of the male partner on the reproductive outcome in intracytoplasmic sperm injection (ICSI) treatment cycles. For this prospective study (ClinicalTrials.gov identifier: NCT02703584), blood and semen samples of the male partner of the 75 women who had ICSI were analyzed. The study group consisted of men who had ICSI for male factor infertility other than azoospermia, while men with normal spermiogram whose partners underwent ICSI due to tubal factor infertility were taken as the study group. Habitual consumption of drinking water from plastic carboys/bottles (PBW) at home was also questioned in both groups as it was considered as chronic BPA exposure. The association of ICSI outcome with blood BPA (bBPA) and semen BPA (sBPA) levels was analyzed in both groups. No significant correlation was found between sperm parameters and bBPA levels in both groups. A negative correlation was found between sBPA levels and total sperm count and progressive sperm motility in men who consumed PBW. Embryo development arrest was found to be significantly higher in patients who have high sBPA levels. Although sBPA levels were not different in PBW consumers, bBPA levels were found to be significantly lower in those who consumed tap water (TW) than those who used PBW. Elevated bBPA were associated with a significant decrease in clinical pregnancy rate. Considering the widespread human exposure to BPA, the effect of BPA on the male reproductive system needs to be further examined. [ABSTRACT FROM AUTHOR]

Aim: Recurrent pregnancy loss (RPL) is a common clinical reproductive problem. With research advancements, an increasing number of studies have suggested that male factors play an important role in RPL. However, the evaluation results of male sperm quality in published meta‐analyses are inconsistent. We aimed to summarize the evidence of the association between semen factors and RPL and evaluate the level and validity of the evidence. Methods: We searched PubMed, Cochrane Library, EMBASE, Web of Science, and Scopus databases for systematic reviews or meta‐analyses to evaluate the association between male semen parameters and RPL. The methodological quality of the included meta‐analyses was assessed, and data and evidence were re‐synthesized and stratified using a random‐effects model. Results: Seven meta‐analyses and nine semen parameters were included in the final analysis. The methodological quality of all publications was considered low or very low. There was highly suggestive evidence for the association between sperm DNA fragmentation (SDF), sperm progressive motility rate, and RPL (class II). The evidence level for the association between sperm concentration, normal sperm morphology, sperm deformity rate, total motility, and RPL was suggestive evidence (class III). The evidence level for the association between sperm volume and sperm count and RPL was weak (class IV). There was no significant association between sperm pH and RPL (class NS). Conclusions: Our results suggest level II evidence for the association between male SDF and RPL, while the evidence level for the association between conventional semen routine parameters and RPL was low (classes III and IV). [ABSTRACT FROM AUTHOR]

Coronavirus disease 2019, which is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), remains an ongoing global public health challenge. This disease causes damage not only to the respiratory system, affecting the normal physiological function of the lungs, but also to other vital organs, such as the heart and testicles. Existing studies have shown that co-expression of angiotensin-converting enzyme 2 and transmembrane serine protease 2 is the main mechanism by which SARS-CoV-2 invades host cells. Angiotensin-converting enzyme 2-expressing cells are widespread in the corpus cavernosum, reproductive tract and testis of men, which has raised concerns. Furthermore, abnormal sex hormone levels and decreased semen parameters were observed in coronavirus disease 2019 patients. This study comprehensively assessed the effects of SARS-CoV-2 infection on the testis, semen parameters, sex hormone levels and erectile function, and discussed possible transmission routes during sexual intercourse and the effect of vaccination on male fertility. Since the outbreak of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic in December 2019, coronavirus disease 2019 (COVID-19) has already had a widespread impact on fertility. This study comprehensively assessed the effects of SARS-CoV-2 infection on the testis, semen parameters, sex hormone levels and erectile function, and discussed possible transmission routes during sexual intercourse and the effect of vaccination on male fertility. It provides a reference for maintaining male reproductive health under the background of normalised prevention and control of COVID-19. [ABSTRACT FROM AUTHOR]

Background: L-carnitine therapy for idiopathic sperm abnormalities exhibits variable effectiveness, and currently, there are no established criteria to predict patient response. This study investigated correlations between seminal plasma markers and semen parameters to identify biomarkers that can guide indications for L-carnitine therapy indications in patients with idiopathic sperm abnormalities. Methods: A retrospective review was conducted on 223 male patients with idiopathic oligoasthenoteratospermia, who sought medical attention at our clinic between January 2020 and October 2022. These patients underwent a pretreatment seminal plasma biochemical analysis, followed by a three-month continuous L-carnitine treatment. The correlation between seminal plasma biochemical parameters and pretreatment semen parameters was analyzed. Semen quality was compared between cases with normal and abnormal seminal plasma biochemical parameters, both pretreatment and posttreatment. The correlation between the changes in semen parameters after treatment and seminal plasma biochemical parameters were investigated. Results: Correlation analyses revealed significant associations between all pretreatment semen parameters and seminal plasma biochemical markers, except for liquefying time and the ratio of normal morphology. Subgroup analysis, stratified by seminal fructose, zinc, citric acid, and neutral glycosidase levels, demonstrated that abnormal groups exhibited significantly different levels of semen parameters compared with the normal groups. The changing difference and changing ratio in the ratio of forward motile sperm showed a negative correlation with seminal fructose levels (r=-0.165 and -0.144). The changing difference in semen volume was negatively correlated with the level of seminal neutral glycosidase (r=-0.158). The changing ratio in semen volume, sperm concentration, total sperm count, and count of forward motile sperm all exhibited negative correlations with the levels of seminal neutral glycosidase (range from -0.178 to -0.224). Conclusion: Seminal plasma biochemical markers, particularly fructose and neutral glycosidase, may serve as valuable indicators for determining the eligibility of patients with idiopathic sperm abnormalities for L-carnitine therapy [ABSTRACT FROM AUTHOR]

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Background: The inactivated severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccine has made significant contributions to fighting the epidemic in the past three years. However, the rapid development and application raised concerns about its safety in reproductive health, especially after several studies had observed a decrease in semen parameters following two doses of mRNA SARS-CoV-2 vaccination. Thus, it is necessary to comprehensively evaluate the effect of inactivated SARS-CoV-2 vaccine on male fertility. Methods: A retrospective cohort study was conducted in the Center for Reproductive Medicine of the Affiliated Hospital of Jining Medical University between July 2021 and March 2023. A total of 409 men with different vaccination status and no history of SARS-CoV-2 infection were included in this study. Their sex hormone levels and semen parameters were evaluated and compared separately. Results: The levels of FSH and PRL in one-dose vaccinated group were higher than other groups, while there were no significant changes in other sex hormone levels between the control and inactivated SARS-CoV-2 vaccinated groups. Most semen parameters such as volume, sperm concentration, total sperm count, progressive motility and normal forms were similar before and after vaccination with any single dose or combination of doses (all P > 0.05). Nevertheless, the total motility was significantly decreased after receiving the 1 + 2 doses of vaccine compared to before vaccination (46.90 ± 2.40% vs. 58.62 ± 2.51%; P = 0.001). Fortunately, this parameter was still within the normal range. Conclusion: Our study demonstrated that any single dose or different combined doses of inactivated SARS-CoV-2 vaccination was not detrimental to male fertility. This information could reassure men who want to conceive after vaccination and be incorporated into future fertility recommendations. [ABSTRACT FROM AUTHOR]

In female increase urea intake may affect negatively in fertility as a result of the change in oviduct acidity this effect of urea noticed also in male semen quality. We expect that inclusion urea instead of soya protein may not affect in semen qualities in male duo to high starch ratio in the ration. The study aimed to find out the effect of partial or total replacement urea as a substitute for soybean meal on the reproductive performance of Awassi male sheep. In this study, 15 Awassi lambs were used with an average initial weight of 60.30 kg, aged 11-13 months. Lambs were randomly divided according to their weight into three groups. The first group (T1) animals were fed a ration containing soybean meal, the second group (T2) 50% of soybean meal was replaced by urea, and the third group (T3) urea was totally replaced with soybean meal. The results showed a feed intake decrease consumed 1.14 kg, in the second treatment compared with the first and third treatments (1.34, 1.34 kg). The average daily and total weight gain 0.116 and 7.00 kg, decreased significantly (P < 0.05) in total replacement of urea compared with partial replacement of urea and control treatment 0.191 and 11.50 kg and 0.174 and 10.50 kg, respectively. Partial replacement urea for soybean meal led to a significant decrease (P < 0.05) in semen characteristics compared with T1 and T2. On the other hand, partial urea replacement led to a significant (P < 0.05) increase in the concentration of blood plasma cholesterol 172.48 mg/dl, compared to the second and third treatments, 134.28 and 137.14 mg/dl, respectively. with feeding starch diets inclusion urea Partially are totally instead of soybean meal has determine effect in semen quality. [ABSTRACT FROM AUTHOR]

The well-documented relationship between chronological age and the sperm methylome has allowed for the construction of epigenetic clocks that estimate the biological age of sperm based on DNA methylation, which we previously termed sperm epigenetic age (SEA). Our lab demonstrated that SEA is positively associated with the time taken to achieve pregnancy; however, its relationship with semen parameters is unknown. A total of 379 men from the Longitudinal Investigation of Fertility and Environment (LIFE) study, a non-clinical cohort, and 192 men seeking fertility treatment from the Sperm Environmental Epigenetics and Development Study (SEEDS) were included in the study. Semen analyses were conducted for both cohorts, and SEA was previously generated using a machine learning algorithm and DNA methylation array data. Association analyses were conducted via multivariable linear regression models adjusting for BMI and smoking status. We found that SEA was not associated with standard semen characteristics in SEEDS and LIFE cohorts. However, SEA was significantly associated with higher sperm head length and perimeter, the presence of pyriform and tapered sperm, and lower sperm elongation factor in the LIFE study (p < 0.05). Based on our results, SEA is mostly associated with defects in sperm head morphological factors that are less commonly evaluated during male infertility assessments. SEA shows promise to be an independent biomarker of sperm quality to assess male fecundity.

The clinical applications of acrosin activity are limited. We analyzed 61 578 male partners in infertile couples who visited the outpatient department of the Reproductive and Genetic Hospital of CITIC-Xiangya (Changsha, China) between August 2014 and December 2019 to determine the reference ranges and thresholds for acrosin activity in infertile Chinese men; to determine whether correlations exist between acrosin activity and age, sperm concentration, sperm morphology, or sperm motility; and to evaluate whether acrosin activity could serve as an effective prognostic indicator for choosing between in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) in the clinic. The cut-off value for the normal reference range of acrosin activity for male partners in infertile couples was 24.78 μIU per 10[6] sperm. There was no significant association between acrosin activity and age, sperm concentration, semen volume, total sperm count, progressive motility, or total motile spermatozoa. A weak positive correlation was found between acrosin activity and normal sperm morphology. There was a statistically significant difference in abnormal acrosome morphology between the group with high acrosin activity (>24.78 μIU per 10[6] sperm) and the group with low acrosin activity (

Objective: Varicocele is the most frequently observed correctable cause of infertility in men. In this study, we aimed to evaluate the influence of age at the time of varicocelectomy on semen parameters and fertility. Methods: Infertile men who underwent microsurgical varicocelectomy between January 2012 and December 2019 were retrospectively evaluated. Patients were divided into 4 age groups as follows: group 1 including patients aged ≤25 years old, group 2 including patients aged ≥26 and ≤30 years old, group 3 including patients aged ≥31 and ≤35 years old, and group 4 including patients aged ≥36 years old. Results: A total of 138 infertile men were divided into 4 groups. There were 18 men in group 1, 58 men in group 2, 44 men in group 3, and 18 men in group 4. There were significant improvements in the mean sperm concentration, progressive motility, and total motile sperm count values in all groups after surgery. Significant improvement in sperm morphology was detected only in groups 1 and 2 (p=0.007 and p=0.005, respectively). There was no significant difference between the groups in terms of preoperative and postoperative sperm parameters. Total fertility rate and the number of patients having children with natural conception or assisted reproductive techniques were lower in group 4 but these differences were not statistically significant (p=0.083 and p=0.454, respectively). Conclusion: Varicocelectomy can be recommended for all infertile men regardless of age. There was no difference in postoperative semen parameters and fertility rates between the age groups.

Abstract Background The Corona Virus Disease 2019 (COVID-19) pandemic has raised concerns regarding its potential impact on male reproductive health. However, the impact of COVID-19 on sperm quality remains uncertain. This retrospective study aimed to investigate the short-term and relatively long-term effects of COVID-19 infection on sperm quality. Methods A total of 85 males with fertility requirements, who underwent semen evaluation at Guilin People’s Hospital between June 2022 and July 2023, were included in the study. Changes in semen parameters were analyzed across three specific timeframes: within 6 months before COVID-19 infection, within 3 months after COVID-19 infection, and 3–6 months after COVID-19 recovery. Results The results revealed that the sperm concentration and total sperm number were significantly lower after infection compared to before, while in the recovery period, the sperm concentration, total sperm count, progressive motility, and normal morphology significantly increased. Comparing the three periods, the most significant difference was observed in sperm concentration, which exhibited a significant decrease after infection but returned to normal levels after recovery from COVID-19. Conclusions These findings suggest that COVID-19 may exert some impact on sperm quality, particularly evidenced by decreased sperm concentration post-infection. Fortunately, these effects on semen parameters appear to be temporary, with gradual restoration of semen parameters within 3–6 months after recovery. However, further research is needed to explore the underlying mechanisms and long-term implications of these observed changes in semen parameters.

Background & aim: At least 50% of infertile couples' problems are related to the male factor infertility. There is widespread use of herbal medicine among men for infertility problems. This study was conducted to determine the effect of Ceratonia siliqua L. (Carob) on semen parameters in idiopathic male infertility.Methods: This systematic review was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA). Articles were searched without time restrictions till September 2023 through searching English databases including PubMed, Scopus, Web of Science, Cochrane Library, EMBASE and ProQuest using MeSH terms of male infertility, RCT, herbal medicine, idiopathic male infertility, Ceratonia siliqua L, Carob, semen parameters and their equivalent words in Persian, which were searched individually or in combination. Two reviewers independently assessed eligibility and quality of included articles and any disagreements were resolved by consensus with a third party.Results: Out of 140 articles, 97 documents were removed due to duplication. After screening 43 remaining articles, 30 articles were excluded based on the title and abstract, and eight articles due to being conducted on animals. One article was the study protocol, which was also excluded. Finally, four articles were included in systematic review. All studies reported Carob is effective in improving some or all parameters of semen including count, motility, and morphology.Conclusion: Considering small number of human studies related to the effect of Carob on semen parameters, conducting more robust clinical trials are recommended.

Context: The COVID-19 pandemic has caused widespread concern about its potential impact on various aspects of human health. Aims: This narrative review aims to summarise the current knowledge about the impact of COVID-19 on sperm quality and its effect on assisted reproductive technology. Methods: In this narrative review, a literature search using the PubMed and MEDLINE databases was conducted to identify relevant original research articles published up to 29 January 2023. Results: Thirty original studies were included in our review. There is evidence that SARS-CoV-2 is detectable in seminal fluid during the acute phase of infection and for up to 1 month. However, the fact that SARS-CoV-2 is barely detectable in semen makes sexual transmission very unlikely. COVID-19 infection has been associated with the following changes in sperm quality: morphology, altered motility, changed DNA fragmentation-index (DFI), decreased sperm concentration, lower total number of sperm, and a significant increase in leukocytes and cytokines. The effects mostly seem to be reversible and have not been shown to negatively affect the outcome of assisted reproductive technology but should lead to further research concerning the health of the offspring, because a correlation of increased DFI after COVID-19 even 5 months after disease could be assumed. Conclusions: The findings of this narrative review suggest that SARS-CoV-2 may harm sperm quality in the acute phase. Implications: A recovery time of at least 3 months regarding assisted reproductive therapy could be reasonable. Is sperm quality reduced after COVID-19 infection? In everyday counselling, we often have to answer questions like these and advise people who are undergoing assisted reproductive medicine after a COVID-19 infection. Fortunately, although sperm quality is reduced after COVID-19 infection, this effect appears to be reversible, leading to a recommended 3-month recovery period regarding assisted reproductive therapy. Image by Julia Stark. [ABSTRACT FROM AUTHOR]

Background: The Corona Virus Disease 2019 (COVID-19) pandemic has raised concerns regarding its potential impact on male reproductive health. However, the impact of COVID-19 on sperm quality remains uncertain. This retrospective study aimed to investigate the short-term and relatively long-term effects of COVID-19 infection on sperm quality. Methods: A total of 85 males with fertility requirements, who underwent semen evaluation at Guilin People's Hospital between June 2022 and July 2023, were included in the study. Changes in semen parameters were analyzed across three specific timeframes: within 6 months before COVID-19 infection, within 3 months after COVID-19 infection, and 3–6 months after COVID-19 recovery. Results: The results revealed that the sperm concentration and total sperm number were significantly lower after infection compared to before, while in the recovery period, the sperm concentration, total sperm count, progressive motility, and normal morphology significantly increased. Comparing the three periods, the most significant difference was observed in sperm concentration, which exhibited a significant decrease after infection but returned to normal levels after recovery from COVID-19. Conclusions: These findings suggest that COVID-19 may exert some impact on sperm quality, particularly evidenced by decreased sperm concentration post-infection. Fortunately, these effects on semen parameters appear to be temporary, with gradual restoration of semen parameters within 3–6 months after recovery. However, further research is needed to explore the underlying mechanisms and long-term implications of these observed changes in semen parameters. [ABSTRACT FROM AUTHOR]

A growing interest has been drawn to the use of traditional medicinal plants for the treatment of human diseases and, in particular, infertility and reproductive toxicity associated with environmental factors. The Mediterranean basin area is a recognized source of plant species with therapeutic interest. In this frame, Eruca sativa (ES) is an annual edible plant and a member of the Brassicaceae family. A relatively large number of studies, focusing on the biological effects of the extract from the leaves of ES on in vitro and in vivo models of disease, have been published in recent years. The present narrative review aims to analyze the phytochemical constituents, traditional uses, possible pharmacological activities, and recognized effects of ES on male reproductive outcomes. Available investigations have revealed the presence of a number of compounds with antioxidant properties, such as polyphenols, glucosinolates, flavonoids, and carotenoids in extracts from ES. Based on the chemical and pharmacological characteristics of the aforementioned compounds, we show that ES has possible preventive properties and therapeutic uses, especially in the functional derangements of the male reproductive system. [ABSTRACT FROM AUTHOR]

Objective: Varicocele is the most frequently observed correctable cause of infertility in men. In this study, we aimed to evaluate the influence of age at the time of varicocelectomy on semen parameters and fertility. Methods: Infertile men who underwent microsurgical varicocelectomy between January 2012 and December 2019 were retrospectively evaluated. Patients were divided into 4 age groups as follows: group 1 including patients aged ≤25 years old, group 2 including patients aged ≥26 and ≤30 years old, group 3 including patients aged ≥31 and ≤35 years old, and group 4 including patients aged ≥36 years old. Results: A total of 138 infertile men were divided into 4 groups. There were 18 men in group 1, 58 men in group 2, 44 men in group 3, and 18 men in group 4. There were significant improvements in the mean sperm concentration, progressive motility, and total motile sperm count values in all groups after surgery. Significant improvement in sperm morphology was detected only in groups 1 and 2 (p=0.007 and p=0.005, respectively). There was no significant difference between the groups in terms of preoperative and postoperative sperm parameters. Total fertility rate and the number of patients having children with natural conception or assisted reproductive techniques were lower in group 4 but these differences were not statistically significant (p=0.083 and p=0.454, respectively). Conclusion: Varicocelectomy can be recommended for all infertile men regardless of age. There was no difference in postoperative semen parameters and fertility rates between the age groups. [ABSTRACT FROM AUTHOR]

(1) Background: We aimed to investigate the effect of change in pre-wash and post-wash semen parameters on intrauterine insemination (IUI) success in a homogenous study group. (2) Methods: IUI cycles conducted at an infertility clinic were included in this study. Patient records were examined retrospectively. Δ sperm count (per mL) was calculated as [pre-wash sperm count (per mL)–post-wash sperm count (per mL)]. Δ Total progressive motile sperm count (TPMSC) was also calculated as (post-wash TPMSC-pre-wash TPMSC). (3) Results: No statistically significant difference was detected in terms of Δ sperm count (p = 0.38), and Δ TPMSC (p = 0.76) regarding the clinical pregnancy rate (CPR). There was no statistically significant difference between CPR (+) and CPR (−) groups in terms of post-wash sperm count, TPMSC, TPMSC ≥ 10 × 10⁶, TPMSC ≥ 5 × 10⁶ (p = 0.65, p = 0.79, p = 0.49, p = 0.49, respectively). The live birth rate (LBR) showed no statistically significant differences except for a pre-wash TPMSC ≥ 10 × 10⁶ (p = 0.02). Through the performed ROC analysis, no statistically significant cutoff value could be set for the pre-wash TPMSC. (4) Conclusions: There is only a pre-wash TPMSC ≥ 10 × 10⁶ that showed a significant role in the success of IUI, even when considering all other pre-wash and post-wash semen parameters. Δ sperm count and Δ are not useful markers for IUI success. [ABSTRACT FROM AUTHOR]

One type of epigenetic modification is genomic DNA methylation, which is induced by smoking, and both are associated with male infertility. In this study, the relationship between smoking and CHD5 gene methylation and semen parameters in infertile men was determined. After the MS‐PCR of blood in 224 samples, 103 infertile patients (62 smokers and 41 non‐smokers) and 121 fertile men, methylation level changes between groups and the effect of methylation and smoking on infertility and semen parameters in infertile men were determined. The results showed that there is a significant difference in the methylation status (MM, MU, UU) of the CHD5 gene between the patient and the control group, and this correlation also exists for the semen parameters (p <.001). The average semen parameters in smokers decreased significantly compared to non‐smokers and sperm concentration was (32.21 ± 5.27 vs. 55.27 ± 3.38), respectively. MM methylation status was higher in smokers (22.5%) compared to non‐smokers (14.6%). Smoking components affect the methylation pattern of CHD5 gene, and smokers had higher methylation levels and lower semen parameters than non‐smokers, which can be biomarkers for evaluating semen quality and infertility risk factors. Understanding the epigenetic effects of smoking on male infertility can be very useful for predicting negative consequences of smoking and providing therapeutic solutions. [ABSTRACT FROM AUTHOR]

Background and objective—Obesity is a prevalent health concern that notably impairs male fertility through hormonal disruptions and other pathophysiological alterations. Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) can significantly reduce weight. This narrative review synthesizes the existing literature discussing the impact of glucagon-like peptide-GLP-1 RAs on the male reproductive system, particularly on the hypothalamic–pituitary–gonadal axis and spermatogenesis, highlighting their potential impact on male fertility. Material and methods—PubMed database was used for the retrieval of English-language articles published up to November 2023. This non-systematic literature review predominantly concentrates on both pre-clinical and clinical studies pertaining to GLP-1 RAs, specifically exploring their impact on male reproductive hormones and sperm parameters. Results—GLP-1 receptors have been identified within the male reproductive system according to the existing literature. While the exact mechanisms are not well understood, they appear to be involved in glucose homeostasis and energy metabolism, both vital processes in spermatogenesis. Multiple clinical trials have demonstrated the efficacy of GLP-1 RAs for promoting weight loss. Recent studies show that the use of GLP-1 RAs in obese males may enhance sperm metabolism, motility, and insulin secretion in vitro, along with positive effects on the human Sertoli cells. Recent clinical trials discussed in this review demonstrate weight loss associated with GLP-1 RAs is correlated with improvements in sperm count, concentration, and motility. However, the direct impact of GLP-1 RAs on male reproductive hormones remains unclear, necessitating further research to confirm their potential role in treating male infertility. Conclusions—This narrative review summarizes the existing literature discussing the potential impact of GLP-1 RA on the male reproductive system, emphasizing their potential therapeutic role in addressing idiopathic infertility in obese men. Despite numerous studies exploring the influence of GLP-1 and GLP-1 RAs on reproductive hormones, testicular function, and spermatogenesis, further clinical trials are crucial to validate initial evidence. Longer follow-up periods are essential to address uncertainties regarding the long-term repercussions and outcomes of GLP-1 RA use. While this holds true, the current literature suggests that GLP-1RAs show promise as a potential therapeutic approach for improving sperm parameters in obese men. [ABSTRACT FROM AUTHOR]

Donor sperm treatment is advised to be performed with frozen-thawed donor semen. A disadvantage of frozen-thawed semen is lower pregnancy rates compared to inseminations with fresh semen. Semen parameters affect ongoing pregnancy rates in intracervical inseminations with frozen-thawed donor semen. In an attempt to translate this into clinical relevance, cohort studies have tried to find cut-off values for semen parameters after thawing for intracervical insemination, but these studies assessed only one semen parameter per study, thereby overlooking the intricate interplay between all semen parameters. We performed a retrospective cohort study and tried to calculate thresholds for all semen parameters that lead to the best possible ongoing pregnancy rates in intracervical insemination with frozen-thawed donor semen. Between April 1999 and December 2015, data from 1,186 women who underwent 7,103 cycles of intracervical insemination with donor semen from 129 sperm donors were available for analysis. Our results showed that total motility and total motile count (TMC) after thawing were associated with ongoing pregnancy rate. The best possible ongoing pregnancy chances after intracervical insemination were obtained at a total motility of ≥20% and a total motile count (TMC) of ≥8 × 106 after thawing. [ABSTRACT FROM AUTHOR]

Seasonal variations in semen parameters play a critical role in the reproductive efficiency and fertility of domestic animals. Factors such as temperature, photoperiod, and humidity directly impact sperm concentration, viability, motility, morphology, seminal plasma composition, and other semen parameters. Understanding these seasonal variations in semen parameters is crucial for optimising reproductive management practices in domestic animal populations. This comprehensive review provides valuable insights for improving breeding programs, artificial insemination techniques, and overall reproductive management in domestic animals. [ABSTRACT FROM AUTHOR]

The impact of paternal obesity and metabolic disease on semen quality and fertility outcomes is not fully appreciated. With increasing obesity rates, researchers have studied the intricate relationship between paternal body mass index, metabolic health, and male fertility. This systematic review identified 112 articles in the MEDLINE database between 2013 and 2023 that investigated the effects of body mass index, diabetes, metabolic syndrome, exercise, weight loss medication, or bariatric surgery on semen parameters, sperm quality, or fertility outcomes. This review suggests that obesity, diabetes, and metabolic syndrome have a negative impact on various parameters of male fertility, from semen quality to sperm deoxyribonucleic acid integrity. There is also mounting evidence that male obesity is correlated negatively with live births via both natural conception and assisted reproductive technologies. Lifestyle interventions, such as physical exercise, generally appear to improve male fertility markers; however, the type and intensity of exercise may play a crucial role. Pharmacologic treatments for weight loss, such as metformin and glucagon-like peptide 1 agonists, present a more complex picture, with studies suggesting both beneficial and detrimental effects on male reproductive health. Similarly, surgical interventions, such as gastric bypass surgery, show promise in improving hormonal imbalances but have mixed effects on semen parameters. Future research is needed to clarify these associations and inform clinical guidelines. In the interim, health practitioners should incorporate these insights into clinical practices, encouraging proactive lifestyle changes and providing targeted treatments to improve male reproductive health. [ABSTRACT FROM AUTHOR]

Vitamin A has multiple functions in the human body, being involved in growth, epithelial differentiation, vision, immune function and reproduction. While normal spermatogenesis is influenced by several factors, it requires vitamin A. Systemic isotretinoin is a vitamin A derivative that is used in the treatment of many dermatological diseases, especially acne vulgaris (AV). There is limited research on the changes in semen parameters after systemic isotretinoin therapy in humans. Our study investigates the presence of varicoceles in patients undergoing systemic isotretinoin therapy for AV and examines whether there were any changes in the semen parameters before and after treatment. Included in the study were 46 men patients who were scheduled for systemic isotretinoin therapy for AV. Before treatment, the patients underwent a physical examination and ultrasonography for varicoceles assessment. The patients underwent spermiogram before treatment and after 6 months of treatment. The spermiogram assessments included semen volume, sperm concentration, total sperm count, progressive motility, viability and sperm morphology. After treatment, there was an increase in semen volume, sperm concentration, total sperm count, progressive motility and vitality from the pre-treatment values, but a deterioration in the sperm morphology (p <.05). Comparing patients with and without varicoceles revealed more changes in semen parameters after treatment in those with varicoceles. There was a statistically significant difference in sperm concentration (p <.001). Systemic isotretinoin therapy negatively affects sperm morphology, but has positive effect on other semen parameters, and these changes in semen parameters occur more frequently in patients with varicoceles. Acne vulgaris is a very common disease and systemic isotretinoin is used as the most effective agent in its treatment. Systemic isotretinoin positively affects semen parameters except sperm morphology. Changes in semen parameters are more common in patients with varicocele. [ABSTRACT FROM AUTHOR]

PurposeThis study aimed to investigate the effects of ejaculatory abstinence on sperm parameters.MethodsThis analysis was registered in PROSPERO (CRD42023472124). We performed a search on PubMed using the following text terms: ((“sperm parameters” OR “sperm analysis” [Mesh]) AND (“sperm DNA fragmentation” OR “DNA fragmentation” [Mesh]) AND (“sexual abstinence” [Mesh] OR “abstinence”)) and an advanced search in Scopus using the terms (“sperm parameters” OR “sperm parameters” OR “DNA fragmentation”) AND (“abstinence”). The sperm parameters that were investigated were sperm volume, total sperm motility, progressive sperm motility, sperm concentration, sperm morphology, and sperm DNA fragmentation (SDF). A two-day cut-off as a “short” or “long” abstinence period has been defined.ResultsThirteen studies published between 2013 and 2022 were included in this meta-analysis. A total of 2,315 patients, ranging from 6 to 836 from each cohort, were enrolled in the study. We showed that longer abstinence time was associated with greater sperm concentration (mean difference [MD]: 8.19; p

Objective: This study aims to investigate the current evidence regarding the impact of oral antioxidant supplementation on semen parameters of infertile men. Materials and Methods: We conducted a systematic search of PubMed, and Cochrane electronic databases, adhering to modified Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. The focus was on studies exploring the effects of antioxidant therapy on infertile men, with an examination of antioxidants in terms of types, doses, rationale for use, and their impact on semen parameters measures. Results: A total of 18 studies that met the inclusion criteria were included in this study. Out of these, 14 studies reported a significantly positive influence of antioxidant therapy on basic semen parameters and advanced sperm function. These comprised 11 randomized clinical trials and 7 prospective studies. Commonly utilized antioxidants included Vitamin E, Vitamin C, carnitines, co-enzyme Q10, N-acetyl cysteine, zinc, selenium, folic acid, and lycopene. Conclusions: Overall, antioxidants generally demonstrate a favorable effect on semen parameters of infertile men. However, further research is necessary to pinpoint the optimal antioxidant regimen that can be applied safely and effectively in clinical practice.

Background: Bisphenol A (BPA) is known as an endocrine disruptor that has harmful effects on general health. It iscommonly used in various industrial products. In this study we tried to evaluate the amount of BPA in urine samplesof the men referred to an infertility center.Materials and Methods: The cross-sectional study population consisted of male partners of infertile couples, who werereferred to infertility clinic in Mazandaran, a northern state of Iran. Questionnaires included demographic characteristics,medical history, lifestyle factors, physical examinations. A semen sample and a spot urine sample were taken from eachparticipant. In the initial study group of 240 men, 3 groups were excluded, and 122 men remained for the analysis. Highperformanceliquid chromatography (HPLC) was applied to measure the amount of BPA in the urine samples.Results: BPA was not detected in about half of the samples (53.3%). Multiple linear regression analysis showed thatno significant relationship existed between the urine concentrations of BPA, semen parameters and male reproductivehormones. However, in a comparison with semen parameters in people with detectable urine BPA versus nondetectableones, an inverse association was noticed with sperm concentration. In other parameters, differences were notsignificant. Smoking had no effects on sperm parameters, but body mass index (BMI) ≥25 reduced the percentage ofnormal sperm parameters.Conclusion: In most participants, urinary BPA was not detected. Probably in this study low environmental exposure toBPA is the cause of lower urine BPA concentrations compared to other industrially developed countries. Therefore, nooverall relationship was observed between BPA level and male infertility.