1. Selenized Plant Oil Is an Efficient Source of Selenium for Selenoprotein Biosynthesis in Human Cell Lines
- Author
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Katarzyna Bierla, Karolina Modzelewska, Maurine Mosca, Elżbieta Anuszewska, Jordan Sonet, Anne-Laure Bulteau, Iza Ksiazek, Joanna Szpunar, Piotr Suchocki, Laurent Chavatte, Ryszard Lobinski, Anna Flis-Borsuk, Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), ANR-09-BLAN-0048,SELENOPROTEOME(2009), Medical University of Warsaw - Poland, National Medicines Institute - Narodowy Instytut Leków [Warsaw] (NIL), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Lobinski, Ryszard, Blanc - - SELENOPROTEOME2009 - ANR-09-BLAN-0048 - Blanc - VALID, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,GPX1 ,Antioxidant ,medicine.medical_treatment ,selenoprotein ,Selenic Acid ,GPX4 ,Selenious Acid ,chemistry.chemical_compound ,0302 clinical medicine ,Selenium Compounds ,Selenoproteins ,selenium ,[SDV.BBM.BC] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,chemistry.chemical_classification ,Nutrition and Dietetics ,Selenocysteine ,[CHIM.MATE]Chemical Sciences/Material chemistry ,Amino acid ,[CHIM.THEO]Chemical Sciences/Theoretical and/or physical chemistry ,Biochemistry ,030220 oncology & carcinogenesis ,lcsh:Nutrition. Foods and food supply ,[CHIM.POLY] Chemical Sciences/Polymers ,Selol ,[CHIM.ANAL] Chemical Sciences/Analytical chemistry ,Txnrd2 ,Txnrd1 ,chemistry.chemical_element ,lcsh:TX341-641 ,Selenate ,Article ,03 medical and health sciences ,[CHIM.ANAL]Chemical Sciences/Analytical chemistry ,Cell Line, Tumor ,medicine ,Humans ,Plant Oils ,ICP-MS ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,Triglycerides ,[CHIM.MATE] Chemical Sciences/Material chemistry ,selenized lipids ,[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition ,[CHIM.THEO] Chemical Sciences/Theoretical and/or physical chemistry ,030104 developmental biology ,HEK293 Cells ,[CHIM.POLY]Chemical Sciences/Polymers ,chemistry ,Gpx1 ,Selenoprotein ,[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition ,Gpx4 ,Selenium ,Food Science - Abstract
International audience; Selenium is an essential trace element which is incorporated in the form of a rare amino acid, the selenocysteine, into an important group of proteins, the selenoproteins. Among the twenty-five selenoprotein genes identified to date, several have important cellular functions in antioxidant defense, cell signaling and redox homeostasis. Many selenoproteins are regulated by the availability of selenium which mostly occurs in the form of water-soluble molecules, either organic (selenomethionine, selenocysteine, and selenoproteins) or inorganic (selenate or selenite). Recently, a mixture of selenitriglycerides, obtained by the reaction of selenite with sunflower oil at high temperature, referred to as Selol, was proposed as a novel non-toxic, highly bioavailable and active antioxidant and antineoplastic agent. Free selenite is not present in the final product since the two phases (water soluble and oil) are separated and the residual water-soluble selenite discarded. Here we compare the assimilation of selenium as Selol, selenite and selenate by various cancerous (LNCaP) or immortalized (HEK293 and PNT1A) cell lines. An approach combining analytical chemistry, molecular biology and biochemistry demonstrated that selenium from Selol was efficiently incorporated in selenoproteins in human cell lines, and thus produced the first ever evidence of the bioavailability of selenium from selenized lipids.
- Published
- 2019
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