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Seed coating is commonly applied in agricultural seed industry to alleviate biotic and edaphic barriers and improve seed germination, seedling emergence and establishment. Recently, this seed enhancement technology has been tested and applied to seeds of native species for ecological restoration and conservation. This work presents a novel application of seed coating for the reintroduction of species with dust‐like seeds that render direct seeding and cultivation unfeasible. Here we have used seed coating on Lindernia procumbens (Krocker) Philcox (Linderniaceae), a threatened annual species of paddy fields. Lindernia procumbens seeds were encrusted to the surface of rice seeds that acted as carrier to spread the species along rice sowing. We first tested how two selected concentrations of binder affected L. procumbens germination, then we investigated sowing depth (i.e. surface level—light, and buried—darkness) effect. Rice encrusting is a feasible and valuable tool for preserving threatened species with tiny seeds, since binder did not limit L. procumbens germination. Light increased L. procumbens seedlings emergence compared to buried conditions. Although L. procumbens emergence was limited in a paddy field trial, we promoted the formation of a soil seed bank for the species. As such, this should not discourage the possibility to reintroduce rare species with tiny seeds in agroecosystems. This pioneering technique can be extended to ecological restoration, where there is an urgent need for new seed‐based approaches and seed‐coating technologies to improve seedling establishment and restoration efficacy. [ABSTRACT FROM AUTHOR]

Salt stress is one of the most important abiotic stress factors limiting crop production. Therefore, improving the stress resistance of seeds is very important for crop growth. Our previous studies have shown that using microcapsules encapsulating bacteria (Pontibacter actiniarum DSM 19842) as seed coating for wheat can alleviate salt stress. In this study, the genes and pathways involved in the response of wheat to salt stress were researched further. The results showed that compared with the control, the coating can improve osmotic stress and decrease oxidative damage by increasing the content of proline (29.1%), the activity of superoxide dismutase (SOD) (94.2%), peroxidase (POD) (45.7%) and catalase (CAT) (3.3%), reducing the content of hydrogen peroxide (H2O2) (39.8%) and malondialdehyde (MDA) (45.9%). In addition, ribonucleic acid (RNA) sequencing data showed that 7628 differentially expressed genes (DEGs) were identified, and 4426 DEGs up-regulated, 3202 down-regulated in the coated treatment. Many DEGs related to antioxidant enzymes were up-regulated, indicating that coating can promote the expression of antioxidant enzyme-related genes and alleviate oxidative damage under salt stress. The differential gene expression analysis demonstrated up-regulation of 27 genes and down-regulation of 20 genes. Transcription factor families, mostly belonging to bHLH, MYB, B3, NAC, and WRKY. Overall, this seed coating can promote the development of sustainable agriculture in saline soil. [ABSTRACT FROM AUTHOR]

There are many challenges in cotton cultivation, which are mainly linked to management practices and market demands. The textile commerce requirements are increasing but the effects of climate change on cotton cultivation are becoming an issue, as its commercial development depends significantly on the availability of favorable climatic parameters and the absence of insect pests. In this research, it was studied whether the use of two commercial strains as cotton seed coatings could effectively contribute to the previous obstacles. The experiment was carried out in semi-field conditions at the University of Ioannina. It used a completely randomized design and lasted for 150 days. The following treatments were tested: (a) coated seeds with a commercial strain of Beauveria bassiana (Velifer®); (b) coated seeds with a combination of Velifer® and a commercial strain of Beauveria bassiana (Selifer®); and (c) uncoated cotton seeds (control). The biostimulant effect of the two seed coatings was assessed against the growth characteristics of cotton, and the total chlorophyll and proline content. The bioinsecticidal effect was evaluated by measuring the population of Aphis gossypii on the cotton leaves. The proline effect increased by 15% in the treated plants, whereas the total chlorophyll was higher in the use of both Velifer® and Velifer®–Selifer® treatments by 32% and 19%, respectively. Aphid populations also decreased in the treated plants compared to the control plants (29.9% in Velifer® and 22.4% in Velifer®–Selifer®). Based on an assessment of the above parameters, it follows that the two seed coatings can significantly enhance the growth performance of cotton and reduce the abundance of A. gossypii. [ABSTRACT FROM AUTHOR]

This study aims to investigate the impact of sustained delivery of bacterial culture through polymeric nanofibers on rice seed coating — to further enhance rice output, crop yield, and soil improvement in a particular area. A bacterial species, identified as Paenibacillus IBGE-MAB1 through 16S rRNA analysis, was isolated from fertile soil cultivated with rice. The electrospinning technique was used to immobilize bacteria with nanofibers. The SEM and FTIR spectroscopy tests were used to observe morphology and chemical changes in nanofibers. The viability test and release profile from nanofibers before and after coating were evaluated. The cumulative impact of nanofibers coating was evaluated by observing germination and vegetative growth in IRRI-6 and IRRI-8 rice seeds. The Paenibacillus-immobilized nanofibers measured 355.3 ± 67.5 nm in size, showing no change in functional groups. The viability test of bacteria after immobilization in nanofibers indicated a high survival rate of 98%. Coated seeds IRRI-6 and IRRI-8 exhibited a significant improvement in the germination index, up to twice as compared to the control. The higher Vigor index of IRRI-6 and IRRI-8 was measured at 1253 and 1173, respectively. This study presents a novel technique for coating rice seeds with Paenibacilus-immobilized polymeric nanofibers, demonstrating its efficacy as an environmentally friendly technology. The coated seeds exhibited enhanced germination and vegetative growth in rice crops. [ABSTRACT FROM AUTHOR]

Abstract Salt stress is one of the most important abiotic stress factors limiting crop production. Therefore, improving the stress resistance of seeds is very important for crop growth. Our previous studies have shown that using microcapsules encapsulating bacteria (Pontibacter actiniarum DSM 19842) as seed coating for wheat can alleviate salt stress. In this study, the genes and pathways involved in the response of wheat to salt stress were researched further. The results showed that compared with the control, the coating can improve osmotic stress and decrease oxidative damage by increasing the content of proline (29.1%), the activity of superoxide dismutase (SOD) (94.2%), peroxidase (POD) (45.7%) and catalase (CAT) (3.3%), reducing the content of hydrogen peroxide (H2O2) (39.8%) and malondialdehyde (MDA) (45.9%). In addition, ribonucleic acid (RNA) sequencing data showed that 7628 differentially expressed genes (DEGs) were identified, and 4426 DEGs up-regulated, 3202 down-regulated in the coated treatment. Many DEGs related to antioxidant enzymes were up-regulated, indicating that coating can promote the expression of antioxidant enzyme-related genes and alleviate oxidative damage under salt stress. The differential gene expression analysis demonstrated up-regulation of 27 genes and down-regulation of 20 genes. Transcription factor families, mostly belonging to bHLH, MYB, B3, NAC, and WRKY. Overall, this seed coating can promote the development of sustainable agriculture in saline soil.

This study focused on evaluating the effectiveness of seed treatments and different sugar beet varieties in controlling flea beetles (Chaetocnema tibialis) and sugar beet weevils (Asproparthenis punctiventris) in Croatia. The field trials were conducted in Vukovar-Sirmia County and targeted the developmental stages of sugar beet from BBCH 12 to BBCH 31. Although the sowing was done within the optimal period, no clear pattern between germination of the seeds and susceptibility was identified as the results showed different responses at different development stages and among the three variants. The experimental design comprised no insecticide, thiamethoxam + tefluthrin, cyantraniliprole, flupyradifurone and Beauveria bassiana + Metarhizium anisopliae. The results show that the treatments with thiamethoxam + tefluthrin effectively reduced pest damage only at the critical stages of development. The current findings suggest that While some of these alternative methods offer good control, they may prove insufficient when applied individually. Hence, integrating them into a comprehensive pest management approach could be necessary for effectively safeguarding sugar beet yields. Further studies should explore potential additive or synergistic benefits to enhance these strategies.

BACKGROUND: Low recovery of conventional fertilizers remains a significant bottleneck for maize production globally. In particular, with phosphate fertilization, zinc (Zn) is prone to precipitation in soil, reducing recovery of both phosphorus (P) and Zn by maize. RESULTS: The present study was designed to investigate the synergistic effect of zinc oxide (ZnO) nanoparticles (NPs) and P on maize crop growth, yield, and nutrient uptake under ZnO seed coating and foliar application in a randomized complete block design. However, plants were subjected to two ZnO NPs levels (0.5 and 12 kg ha−1) amended with two P levels (45 and 90 kg ha−1). ZnO NPs, especially in the form of foliar application, with a P dose of 90 Kg ha−1 significantly (P < 0.05) improved maize crop growth, yield, and nutrient uptake compared with control. In comparison with the control group, plants grown in these conditions absorbed higher levels of Zn and P. Zn uptake rose to 16.34 g ha−1, 137.88 g ha−1, and 166.89 g ha−1 in roots, grains, and stover respectively, and P uptake increased to 0.80 mg kg−1, 10.066 mg kg−1, and 12.17 mg kg−1 respectively. Additionally, seed emergence rate, plant height, and cob length increased by up to 2%, 1177 cm2, and 3.3 cm respectively compared with control. Furthermore, Zn use efficiency was increased up to 38.55% in ZnO NPs foliar application. CONCLUSIONS: Application of ZnO NPs at 0.5 kg ha−1 in the form of foliar application with 90 kg ha−1 P dose produced a more pronounced increment in the parameters studied than ZnO NPs seed coating did. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

This review addresses the issue of replacing manufactured microplastics in seed coatings used in agriculture. Firstly, it focuses on the policy and regulatory actions taken on microplastics at a global level. There is no consensus within the scientific community on the definition of a microplastic and, more generally, on the classification of plastic debris. Nevertheless, several decision schemes have been proposed in an attempt to define the notion of microplastics. The different criteria relevant to this definition, such as the size, physical state, chemical structure, origin, and persistence of microplastics, are discussed, with a comparison being made between the REACH regulation and the scientific literature. Seed production and processing are also discussed, with the functions of seed coatings being explained in order to gain a better understanding of the properties to be considered in a substitution strategy for currently used microplastics. The main challenges are multiple; substitutes must provide the same performance as microplastics: (i) improving the adherence of the treatment to the seed, (ii) distributing the treatment more evenly over the seed, (iii) reducing the amount of dust-off when handling treated seed, and (iv) improving the seed flowability, which is particularly important during the sowing stage, all while preserving the physiological properties of the seed. Substitute polymers are proposed according to the desired performance and functional properties: two main chemical families of biopolymers were identified in the literature: polysaccharides and proteins. Among them, 13 and 6 polymers, respectively, complied with REACH regulation, demonstrating adhesion, dust reduction performances, and preservation of seed physiological quality in particular. This work aims to guide future studies on microplastic substitution in seed coatings, and to highlight research needs in this area. It is based on an analysis and discussion of the literature, identifying and listing potential substitutes. [ABSTRACT FROM AUTHOR]

In this work, we studied the direct and indirect plant protection effects of an Artemisia absinthium essential oil (AEO) on tomato seedlings against Fusarium oxysporum sp. oxysporum radicis lycopersici (Fol). AEO exhibited a toxic effect in vitro against Fol. Additionally, tomato seedlings germinated from seeds pretreated with AEO and grown hydroponically were protected against Fol. Plant disease symptoms, including, water and fresh weight loss, tissue necrosis, and chlorosis were less pronounced in AEO-treated seedlings. AEO also contributed to plant defenses by increasing callose deposition and the production of reactive oxygen species (ROS) on seed surfaces without affecting seed germination or plant development. The essential oil seed coating also primed a durable tomato seedling defense against the fungus at later stages of plant development. RNA-seq and metabolomic analysis performed on seedlings after 12 days showed that the AEO treatment on seeds induced transcriptomic and metabolic changes. The metabolomic analysis showed an induction of vanillic acid, coumarin, lycopene, oleamide, and an unknown metabolite of m/z 529 in the presence of Fol. The StNRPD2 gene, the second largest component of RNA polymerases IV and V directly involved in de novo cytosine methylation by RNA-directed DNA methylation (RdDM), was highly induced in the presence of AEO. The host methionine cycle (MTC) controlling trans-methylation reactions, was also altered by AEO through the high induction of S-adenosyl methionine transferases (SAMts). Our results suggest that AEO treatment could induce de novo epigenetic changes in tomato, modulating the speed and extent of its immune response to Fol. The EO-seed coating could be a new strategy to prime durable tomato resistance, compatible with other environmentally friendly biopesticides. [ABSTRACT FROM AUTHOR]

This study focused on evaluating the effectiveness of seed treatments and different sugar beet varieties in controlling flea beetles (Chaetocnema tibialis) and sugar beet weevils (Asproparthenis punctiventris) in Croatia. The field trials were conducted in Vukovar-Sirmia County and targeted the developmental stages of sugar beet from BBCH 12 to BBCH 31. Although the sowing was done within the optimal period, no clear pattern between germination of the seeds and susceptibility was identified as the results showed different responses at different development stages and among the three variants. The experimental design comprised no insecticide, thiamethoxam + tefluthrin, cyantraniliprole, flupyradifurone and Beauveria bassiana + Metarhizium anisopliae. The results show that the treatments with thiamethoxam + tefluthrin effectively reduced pest damage only at the critical stages of development. The current findings suggest that While some of these alternative methods offer good control, they may prove insufficient when applied individually. Hence, integrating them into a comprehensive pest management approach could be necessary for effectively safeguarding sugar beet yields. Further studies should explore potential additive or synergistic benefits to enhance these strategies. [ABSTRACT FROM AUTHOR]

Pyrolyzed organic matter, commonly referred to as biochar, generally has positive effects on plant growth and can enhance early seedling development. Biochar-based seed coatings may thus be beneficial, specifically under adverse soil conditions such as high soil salinity. Using a series of lab and greenhouse experiments, we evaluated germination and early plant development in radish (Raphinus sativa) seeds coated with conifer-feedstock slow-pyrolysis biochar. Following the precedent of prior studies, polyvinyl acetate (PVAc) was used as the binding agent. Biochar-based seed coatings reduced germination rates and radicle extension in Petri dish trials; however, leaching of coated seeds largely eliminated this effect. PVAc applied in solution inhibited seed germination to a similar extent, suggesting that toxicity of PVAc or chemical products derived from it is the main mechanism for germination inhibition. A meta-analysis of published literature also indicates negative effects of biochar-based seed coatings using PVAc. In greenhouse trials, the same biochar seed coatings improved aspects of plant growth and physiological status relative to controls, specifically enhancing root development. We conclude that biochar-based seed coatings have promise in promoting early seedling growth and potentially in combating salinity stress in agronomic and restoration applications. However, our findings contraindicate PVAc use as a binding agent. Further research is needed to test non-phytotoxic, readily biodegradable binding agents and to optimize biochars and seed coating processes for specific applications in agronomy and ecological restoration. [ABSTRACT FROM AUTHOR]

Rice is a crucial staple crop cultivated extensively in command areas worldwide. In the command areas, the release of water varies due to the inadequate amount of rainfall in the source area and its proper supply. To combat such situations, direct seeding (DS) methods can be opted for; however, lacunae like excess seeds per drop, scattering of seeds, and intercultural operations like thinning, gap filling, and excess weed growth need a clear refinement to enhance the profitability of rice cultivation. Therefore, a research trial was conducted during 2022-24 at Central Farm, Agricultural College & Research Institute, Madurai, to evolve a suitable direct seeding method. The present investigation was laid out under a split-plot design comprising four main plot treatments and four subplot treatments replicated thrice. The main plot treatments comprised different seeding methods (S) and the subplot treatments comprised different seed coating techniques (P). Based on the experimental results, paper roll seeding and DS + Seed coating @ 2% KNO3 fb Bacillus sp. and Azospirillum (PR + P-KN) combination performed better on establishment with grain (6150 kg/ha) and straw yield (7824 kg/ha) respectively. Regarding the B:C ratio, OS-GP + P-KN resulted in higher with 2.64, closely followed by PR + P-KN with 2.61. The least effect was recorded in the combination of round slots in rice drum seeder and dry seeding (RS + DS). Investigating different techniques of direct seeding and seed coating is essential across various regions, particularly in regulated areas where water discharge remains unpredictable. [ABSTRACT FROM AUTHOR]

The development of a seed coating with micronutrient release system is of extreme research importance for sustainable agriculture. In this work, a zinc release system was developed, using zinc-loaded PVA/Zein nanofibers. Solution blow spinning was used to produce nanofibers (average diameter of approximately 160 nm), as demonstrated by field-emission scanning electron microscopy (FE-SEM), with some bead presence, which served as main zinc reservoir, as evidenced by energy-dispersive X-ray spectroscopy (EDX). Biodegradation assay showed the biodegradability of the PVA/Zein nanofibers with over 30% mass loss after 31 days, an important and desired ability for the proposed application. Encapsulation efficiency of the nanofibers was measured at more than 90%. The release profile of zinc-loaded nanofibers was observed to be a two-step release, with an initial burst release (approximately 52% in 3 h) followed by a slow release (approximately 6% in 189 h), which is related to the zinc reservoir in the nanofibers that was observed by EDX and is a profile that is advantageous for the proposed application. Germination assay shows that the seed coating applied to the soybean seeds was effective, with the zinc released from the nanofibers being absorbed by the seeds and, acting as fertilizer, improved some of the analyzed parameters (approximately 24, 59 and 127% increase in shoot length, α-chlorophyll and zinc content, respectively). Results demonstrated that zinc-loaded PVA/Zein nanofibers are a promising material for to be used as seed coating, being able to improve crop performance. [ABSTRACT FROM AUTHOR]

Sustainability in crop production is highly dependent on favorable indicators of germination and seedling establishment. In this research seed coating different treatments data prepared and membership function defined in fuzzy inference system then used rule editor tool for determining parameters weight for achieved the best coating treatment in salinity and drought stress conditions. Seed coating formulations were investigated in laboratory and greenhouse experiments for their potential to increase maximum germination, germination rate, germination uniformity, and seedling growth of sugar beet seeds. Sugar beet seeds were coated with different compounds and combinations, including micro- (Fe, Zn, Cu, Mn, Co, Mo) and macro- (N, P, K) nutrients, humic acid, gibberellic acid, kaolin, and chitosan. Coated and non-coated sugar beet seeds were evaluated for germination and seedling growth after 10 and 21 days, respectively. In total, 30 different treatments were used to assess the effects of seed coating treatments. In laboratory experiments, sugar beet seeds were placed on paper in Petri dishes and maintained in a germinator at 25 °C. Sodium chloride and polyethylene glycol 8000 were used to apply salinity and drought stresses at three levels each and achieve the results of seed coatings on reducing the effects of salinity and drought stresses. To determine the indices related to emergence and establishment, cultivation trays were utilized with four replications for each treatment. The trays were kept inside a greenhouse. Coating treatments significantly improved total germination percentage, germination rate, seedling growth, and uniformity compared with the non-treated controls. In all treatments, polyvinylpyrrolidone was utilized as a binder. The best treatment with respect to germination and seedling growth indices was number 21 (micro and macronutrients, humic acid, gibberellic acid) in salinity and drought stress conditions. The results by fuzzy inference system illustrated that micronutrients, humic acid, and gibberellic acid create the best seed coating for sugar beet seeds, especially when combined at the specified amounts.

ABSTRACT Cobalt (Co) and molybdenum (Mo) are essential elements with a fundamental role in biological nitrogen fixation in legumes. Vitamins such as thiamine, despite being required in small quantities, influence plant growth. This study aimed to assess the efficiency of different doses of thiamine applied as seed treatment, combined or not with Co and Mo, in enhancing the development of soybean crops. The experiment was conducted in a greenhouse according to a randomized block design with a 6 × 2 factorial arrangement, comprising six thiamine doses (0, 10, 25, 50, 100, and 200 mg kg-1) in the presence or absence of Co and Mo at the recommended rate of 100 mL ha-1. At 40 days after sowing, plants were analyzed for root length, root dry weight, shoot length, shoot dry weight, nodule number, and nodule dry weight. Principal component analysis showed that combined application of Co and Mo with thiamine was negatively associated with all variables and that the thiamine doses most positively associated with the analyzed variables were 50 and 200 mg kg-1. In treatments containing thiamine alone, there was a linear direct relationship between thiamine dose and nodule dry weight. Root and shoot lengths and dry weights were highest in plants treated with thiamine only at a dose of 122 mg kg-1. Application of Co and Mo combined with thiamine via seed treatment does not promote the development of soybean crops. Thiamine application is a promising treatment to increase shoot length, root dry weight, and nodule dry weight in soybean.

Soil salinity represents a significant environmental stressor that impairs crop production and yield. A wide range of treatments have been used to reduce the effects of salinity in plants. Among the treatments used, functionalized nanoparticles (NPs) have shown great results. In light of recently demonstrated beneficial effects of chitosan-melatonin nanoparticles (CTS-Mel NPs) and chitosan-salicylic acid nanoparticles (CTS-SA NPs) in mitigating the deleterious consequences of major stress factors and boosting secondary metabolite biosynthesis, the current study aimed to investigate their potential as seed priming coatings to enhance plant performance under both control and salinity stress conditions. For this purpose, CTS (0.1% w/v), Mel (50 µM), SA (0.5 mM), CTS-Mel NPs and CTS-SA NPs were applied as seed priming agents on corn salad (Valerianella locusta) seeds, and subsequently key morphophysiological and biochemical properties were assayed under salinity conditions (0, 30 and 60 mM NaCl). Accordingly, salinity stress caused significant reduction in fresh and dry weights (FW and DW) of leaves, chl a, total chl, SPAD and enhancement in content of proline, phenolics, MDA, as well as protein content, and activities of SOD and CAT antioxidant enzymes. Concerning the phenolic compounds analyzed, salinity stress did not affect the dominant phenolic constituents with the exception of naringine. Regarding the protective effects of the various priming treatments, the adverse effects of salinity stress were ameliorated with the application of most of the applied treatments, and with CTS-Mel NPs in particular, by enhancing biomass, pigments, total phenols, protein, SOD and CAT antioxidant enzymatic activities, as well as the content of some dominant constituents of phenolic profile. CTS-Mel NPs enhanced chlorogenic acid, naringine, o-coumaric acid and catechin hydrate under both control and salinity conditions. Overall, CTS-Mel NPs outperformed CTS-SA NPs as a seed priming coating and could potentially be widely introduced as an innovative, sustainable approach to mitigate the effects of salinity and other abiotic stress conditions in crop plants.

This study evaluated the effects of Bacillus amyloliquefaciens D203 encapsulated in alginate-bentonite coating enriched with titanium nanoparticles on rice germination and Magnaporthe oryzae infection. Seed coating was achieved using the ionic gelation/extrusion method with modifications. The study investigated various concentrations of strain D203 on biomass and bacterial release from the beads, along with its influence on detached leaves. Results showed that treatment with a strain D203 cell suspension (2 × 107 CFU/ml) significantly enhanced root and shoot lengths, despite a slightly lower germination rate (90%) compared to the control. Treatment with a concentration of 1 × 107 proved to be optimum for growth because it maintained germination at 100% and boosted biomass growth of shoot and root by 49.57% and 16.44%, respectively. Colonization by strain D203, explored through seed coating and subsequent resuspension from roots and media, peaked in roots, indicating preferential root zone inhabitation. In vitro studies demonstrated strain D203’s efficacy against M. oryzae at 21 and 40 days, with the 1 × 107 CFU/ml spray significantly reducing disease symptoms. Strain D203 exhibited a preventive effect, especially in early growth stages. The encapsulated form of strain D203 had a control effect comparable to 40% Hexaconazole but less effective than 75% Tricyclazole. Uncoated and encapsulated B. amyloliquefaciens treatments significantly improved sterile rice plant growth over 21 days, with the encapsulated bead treatment surpassing both controls and free cell treatments by days 14 and 21, despite slower initial growth. Encapsulation presents a promising alternative, potentially matching the effectiveness of chemical fungicides, emphasizing the importance of environmentally friendly alternatives in sustainable disease management strategies.

Seed coating techniques had emerged as a promising approach for precise planting and safeguarding crops against environmental stresses. Simultaneously, infrared drying, renowned for its cost-effectiveness and efficiency, held promises for enhancing the quality of drying processes. This investigation delved into the procedures applied to Khao Dawk Mali 105 rice seeds during the coating and drying process using a prototype of the hexagonal drum. The results found that the drying temperature (factor B) and duration of drying time (factor C) had substantial primary effects (p ≤ 0.05), while the drum rotational speed (factor A) did not exert a noteworthy influence. Additionally, meaningful interactions were observed among the factors AB, AC, BC, and ABC. Impressively, it resulted in germination rates of 80.56 and 80.25 %, coupled with germination indices of 84.88 and 85.47 %, when utilizing a drum speed of 20 rpm, a drying temperature of 45 °C, and a drying time of 8 min (R-204508), and a drum speed of 10 rpm, a drying temperature of 45 °C, and a drying time of 8 min (R-104508), respectively. Additionally, the mean germination time amounted to 9.64 days, and the length of the rice seedling stem reached 13.66 cm when employing a drum speed of 20 rpm, a drying temperature of 50 °C, and a drying time of 8 min (R-205008). These discoveries emphasized the potential of the prototype's seed coating and infrared drying technology integration to improve rice germination and subsequent growth. This enhancement could ultimately result in higher yields and increased productivity, especially in small-scale agricultural settings. [ABSTRACT FROM AUTHOR]

The technology of seed coating with superabsorbent polymer (SAP) has the potential to mitigate the negative impact of drought on seed germination and crop establishment. However, their application on the seed surface can affect the effectiveness of pesticides used for seed treatment in the protection against phytopathogens. In our work, the influence of the Aquaholder®Seed polymer coating on the effectiveness of fungicides in the protection of germinating seeds of spring barley cv. Bojos and Laudis against the fungal pathogen Bipolaris sorokiniana was studied. One-half of the seeds were first treated with fungicides, and then a polymer was applied. Fungicide efficacy was evaluated in a Petri dish test and pot test under the pathogen attack. Seed coating with SAP did not negatively affect fungicide efficacy. The percentage of germinated seeds, seedling emergence, plant height, and symptoms of the disease in the fungicide-treated variants were not significantly changed by the SAP application. Moreover, in cv. Laudis, the application of SAP alone partially protected germinating seeds against pathogen attack. The amount of pathogen DNA in plant tissues of cv. Laudis was not significantly different among seed treatments, while in cv. Bojos, the pathogen DNA increased in seeds coated with SAP alone but decreased in combined treatment with fungicides. These results demonstrated that SAP seed coating does not negatively affect the efficacy of fungicides used for seed protection against fungal pathogens. [ABSTRACT FROM AUTHOR]

Questions: Native grasses are widespread with high commercial value and demand across the restoration sector, though their uptake is often hampered by seed‐use challenges. Seed enhancement technologies (SETs) provide a valuable tool for improving plant establishment outcomes for species (such as native grasses) where seed‐based approaches are essential to achieve large‐scale targets. However, due to the increased investment in resources and time associated with application of SETs, their adoption is dependent on the ability of these treatments to consistently provide benefits to one or more demographic life stages, particularly under field conditions. This meta‐analysis addresses the following research question: what are the overall effects of SETs on plant establishment outcomes compared to untreated florets or seeds in native grasses globally? Location: Global. Methods: The effects of six major SET categories (acid treatment, coating, pelleting, flash flaming, priming, and treatment combinations) on germination, emergence, and shoot and root length and biomass were explored. Furthermore, the consistency of the effects of SETs across study types (i.e., laboratory, glasshouse, and field) was investigated. Results: The overall effects of SETs on native grasses in this meta‐analysis were positive for germination (16% improvement), emergence (22% improvement), and growth (6.42–8.86 cm and 2.26–2.77 g increase in seedling length and biomass, respectively). However, effects ranged from neutral to positive when data were grouped by SET type, with coating, pelleting and priming delivering consistent benefits across multiple life stages, and acid treatments, flash flaming, and combination treatments having mixed effects. This analysis also provided evidence that benefits observed from SET application in laboratory and glasshouse studies are not translating to field environments. Conclusions: Overall, SETs generally had positive effects and can therefore provide a relatively low‐risk approach to improving seed‐based restoration outcomes in native grasses. Continued SET research focussed on long‐term plant establishment outcomes in field environments is needed to further advance SET uptake. [ABSTRACT FROM AUTHOR]

Poor seedling emergence often limits the success of direct seeding in ecological restoration. New techniques for maximising seed use efficiency and seedling emergence are needed to help meet global targets for nature repair in the UN Decade on Restoration. Extruded pellets are widely used in agriculture and represent a promising advancement in seed‐based restoration. However, extruded pellets must be optimised for diverse suites of native species that possess a range of seed sizes and morphotypes.We investigated how seed mass affects the performance of native plant seeds (total % seedling emergence) when encapsulated in extruded pellets designed for revegetation. Two glasshouse trials were undertaken using seeds from 30 native Australian plant species. In Trial 1, we encapsulated seeds in the centre of pellets and determined the relationship between seed mass and emergence. In Trial 2, we encapsulated seeds nearer the periphery of pellets and determined whether the position of seeds (central vs. peripheral) affected emergence for a subset of 10 small‐seeded species. In both trials, emergence from pellets was compared to an optimal, bare‐seeded control to identify any barriers to seed encapsulation under well‐watered conditions.In Trial 1, when seeds were centrally encapsulated, emergence was generally higher for bare‐seeded controls relative to pelleted seeds. However, seed mass predicted emergence when seeds were encapsulated in the pellet centre (R2 = 0.32, p = 0.002), such that larger‐seeded species tended to have higher emergence than smaller‐seeded species.In Trial 2, encapsulating seeds nearer the pellet periphery (relative to the centre) resulted in an average 28‐fold increase in emergence for all 10 small‐seeded species. For half of the small‐seeded species trialled, emergence from the pellet periphery was equivalent to that of bare‐seeded controls.Synthesis and applications: Collectively, our results demonstrate: (1) a positive relationship between seed mass and emergence under central‐encapsulation, and (2) that emergence can be significantly improved for small‐seeded species when seeds are positioned nearer the pellet periphery. Translation of these findings into practice can help optimise emergence outcomes for native species with different sized seeds and nuanced germination requirements. [ABSTRACT FROM AUTHOR]

Straw returning to the field has an inhibitory effect on seed germination and the early stage of seedling growth. Nanoparticles may contribute to good seed germination and seedling growth under stress. However, the effects of nanoparticles on seed germination and seedling growth of rice under straw returning remain unknown. Two popular aromatic rice varieties, Xiangyaxiangzhan and Meixiangzhan 2, were used as experimental materials. Three doses of iron oxide nanoparticles (Fe2O3 NPs) (i.e., 0 mg L−1, 25 mg L−1, and 50 mg L−1) were used for seed coating, and the nanoparticle-coated seeds were grown at three concentrations of straw extract (i.e., 0 g L−1, 10 g L−1, and 20 g L−1). The seed germination, seedling growth, antioxidant response, and nitrogen metabolism of aromatic rice were studied. The results showed that the aromatic rice varieties responded differently to straw concentration. Straw extract at 10 mg L−1 promoted the germination of Xiangyaxiangzhan but inhibited the germination of Meixiangzhan 2. With increasing straw extract concentration, the antioxidant response of Xiangyaxiangzhan increased. Fe2O3 NPs significantly increased seed germination under straw stress by 5–44%. The growth response of aromatic rice to iron oxide nanoparticle coating treatments varied among the different aromatic rice varieties and straw extract treatments. Therefore, iron oxide nanoparticle coating improved seed germination and regulated early growth and physiological parameters in aromatic rice. [ABSTRACT FROM AUTHOR]

Beneficial plant microbes can enhance the growth and quality of field crops. However, the benefits of microbes using cheap and efficient inoculation methods are still uncommon. Seed coating with biocontrol agents can reduce the amount of inocula along with having the potential for large-scale application. Hence, in this research work, the comparative potential of tomato seed coating and biopriming with Bacillus aryabhattai Z-48, harboring multiple plant-beneficial traits, to suppress Fusarium wilt disease along with its beneficial effect on seedling and plant growth promotion was analyzed. Among two bacterial strains, B. aryabhattai Z-48 was able to antagonize the mycelial growth of Fusarium oxysporum f.sp. lycopersici in vitro and its application as a seed coating superiorly benefited seedling traits like the germination percentage, vigor index, and seedling growth index along with a reduced germination time. The seed coating with B. aryabhattai Z-48 resulted in significant increases in the shoot length, root length, dry biomass, and total chlorophyll contents when compared with the bioprimed seeds with the same bacterial strain and non-inoculated control plants. The seed coating with B. aryabhattai Z-48 significantly reduced the disease index (>60%) compared with the pathogen control during pot trials. Additionally, the seed coating with B. aryabhattai Z-48 resulted in a significantly higher production of total phenolics, peroxidase, polyphenol oxidase, and phenylalanine ammonia lyase enzyme in tomato plants. The GC/MS-based non-targeted metabolic profiling indicated that the seed coating with B. aryabhattai Z-48 could cause large-scale metabolite perturbations in sugars, sugar alcohols, amino acids, and organic acids to increase the fitness of tomato plants against biotic stress. Our study indicates that a tomato seed coating with B. aryabhattai Z-48 can improve tomato growth and suppress Fusarium wilt disease effectively under conventional agricultural systems. [ABSTRACT FROM AUTHOR]

Copyright of Indonesian Journal of Forestry Research is the property of Indonesian Journal of Forestry Research and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The aim of this study was to obtain a hydrogel coating based on crosslinked alginate enriched with tannery waste-derived biochar and macro- and micronutrients. Sodium alginate (4% wt./wt. and 6% wt./wt.), carboxymethyl cellulose (0.1% wt./wt.), and biochar based on non-chrome waste from the tanning industry (5% wt./wt. and 10% wt./wt.) were used to produce polymeric coatings. The effect of micronutrient concentration (Cu(II), Mn(II), and Zn(II)) in the crosslinking solution on the content of elements in the seed coat was evaluated. SEM–EDS surface analysis showed that the applied method enables/allows uniform coverage of the seeds with the composite. At the same time, the addition of biochar causes significant surface development and slight cracking. The study showed that an alginate coating (6% wt./wt.) with biochar (5% wt./wt.) crosslinked in a 1500 mg/L solution results in a swelling index of 490%. The controlled release of macro- and micronutrients was confirmed in/by in vitro tests. The effectiveness of the coatings was analyzed in germination tests. The addition of biochar was found to have a biostimulatory effect on the growth of the underground parts of the plant (the root length increased by approximately 50% compared to seeds without coating). Seed coating with immobilized biochar can advantageously be commercialized since stimulating early root growth can lead to increased yields. [ABSTRACT FROM AUTHOR]

ABSTRACT: Seed treatment can be carried out in two modalities: On-Farm or via industrial treatment (IST). Evaluating the quality of the treatment is crucial, considering the percentage of coating by the phytosanitary product and the adequacy of the dose. In this context, the aim of this study was to evaluate the use of high-resolution computerized image analysis to assess the coating of treated soybean seeds. In addition, it was sought to characterize the quality profiles of coating and dose adequacy in treated seeds in the Brazilian agribusiness. 150 samples of seed treated in both modalities were collected from various soybean producers in Brazil and evaluated for the percentage of coating by means of color quantification in high-resolution image analysis and for the percentage of the active insecticide ingredient by means of High Performance Liquid Chromatography (HPLC). Image analysis was effective in quantifying coating, regardless of the dominant color. Coating correlated directly with dose effectiveness in the IST modality. Seeds treated via IST tended to have higher quality and homogeneity of treatment compared to On-Farm treated seeds, showing adequate coating and dose effectiveness. On-Farm treated seeds showed heterogeneity in treatment quality, with significant variation in coating and dose effectiveness.

There are many challenges in cotton cultivation, which are mainly linked to management practices and market demands. The textile commerce requirements are increasing but the effects of climate change on cotton cultivation are becoming an issue, as its commercial development depends significantly on the availability of favorable climatic parameters and the absence of insect pests. In this research, it was studied whether the use of two commercial strains as cotton seed coatings could effectively contribute to the previous obstacles. The experiment was carried out in semi-field conditions at the University of Ioannina. It used a completely randomized design and lasted for 150 days. The following treatments were tested: (a) coated seeds with a commercial strain of Beauveria bassiana (Velifer®); (b) coated seeds with a combination of Velifer® and a commercial strain of Beauveria bassiana (Selifer®); and (c) uncoated cotton seeds (control). The biostimulant effect of the two seed coatings was assessed against the growth characteristics of cotton, and the total chlorophyll and proline content. The bioinsecticidal effect was evaluated by measuring the population of Aphis gossypii on the cotton leaves. The proline effect increased by 15% in the treated plants, whereas the total chlorophyll was higher in the use of both Velifer® and Velifer®–Selifer® treatments by 32% and 19%, respectively. Aphid populations also decreased in the treated plants compared to the control plants (29.9% in Velifer® and 22.4% in Velifer®–Selifer®). Based on an assessment of the above parameters, it follows that the two seed coatings can significantly enhance the growth performance of cotton and reduce the abundance of A. gossypii.

Infections of agricultural crops caused by pathogen ic fungi are among the most widespread and harmful, as they not only reduce the quantity of the harvest but also significantly deteriorate its quality. This study aims to develop unique seed-coating formulations incorporating biopolymers (polyhydroxyalkanoate and pullulan) and beneficial microorganisms for plant protection against phytopathogens. A microbial association of biocompatible endophytic bacteria has been created, including Pseudomonas flavescens D5, Bacillus aerophilus A2, Serratia proteamaculans B5, and Pseudomonas putida D7. These strains exhibited agronomically valuable properties: synthesis of the phytohormone IAA (from 45.2 to 69.2 µg mL−1), antagonistic activity against Fusarium oxysporum and Fusarium solani (growth inhibition zones from 1.8 to 3.0 cm), halotolerance (5–15% NaCl), and PHA production (2.77–4.54 g L−1). A pullulan synthesized by Aureobasidium pullulans C7 showed a low viscosity rate (from 395 Pa·s to 598 Pa·s) depending on the concentration of polysaccharide solutions. Therefore, at 8.0%, w/v concentration, viscosity virtually remained unchanged with increasing shear rate, indicating that it exhibits Newtonian flow behavior. The effectiveness of various antifungal seed coating formulations has been demonstrated to enhance the tolerance of barley plants to phytopathogens. [ABSTRACT FROM AUTHOR]

Coating seeds in taste-averting chemical defenses (e.g., capsaicin from Capsicum spp.) can reduce rodent seed predation, and thus might promote plant establishment and forest regeneration. However, the efficacy of such seed coatings remains unknown for many woody plant species, and seed coatings have not been evaluated across different habitats where forest managers might seek to promote forest regeneration. We used two complementary seed-removal experiments in closed-canopy forests (Michigan) and an old field undergoing reforestation (New York) to examine whether coating seeds of four native tree species (Acer rubrum, Fagus grandifolia, Pinus banksiana, and Pinus resinosa) with chili powder reduces seed removal by rodents. In all species and habitats, control seeds were removed more than seeds treated with capsaicin (94% more in the closed-canopy forest, 17% more in the old field). Seed coatings containing capsaicin may provide a generally effective tool to support native tree recruitment and promote restoration success. [ABSTRACT FROM AUTHOR]

Coating seeds with amendments to increase germination, emergence, and establishment is a promising strategy for dryland restoration. Seed coatings containing fungicides offer a potential solution in regions where fungal pathogens cause seed mortality during the winter stratification period between late fall seeding and spring germination. The effectiveness of the fungicide treatment, however, may be dictated by weather and within‐site microenvironment. We tested how fungicide coating influenced seedling emergence of native grasses within sagebrush stands by planting untreated seeds and seeds coated (encrusted) with and without active fungicide ingredients in furrows that extended from the canopy edge of sagebrush plants (Artemisia tridentata ssp. wyomingensis) into the interspace. This was replicated at four sites across the Intermountain West in two successive years. We planted two native grasses, bottlebrush squirreltail (Elymus elymoides) and bluebunch wheatgrass (Pseudoroegneria spicata). Emergence was extremely low in both years, with a complete seeding failure (i.e. zero emergence) at two sites in the first year and three sites in the second year. At one site where emergence was sufficient for statistical analysis: (1) the coating on the fungicide and blank treatments inhibited emergence under anomalously dry conditions and (2) across seed treatments, proximity to a sagebrush canopy slightly increased seedling emergence. The variable emergence patterns across sites and years (i.e. the highest emergence was for the site–year combination with the lowest precipitation) highlight the sensitivity of seeding outcomes to, and dependence of fungicide seed coatings on, site conditions, and the necessity of repeating experiments across different weather years. [ABSTRACT FROM AUTHOR]

Physcion can induce plant resistance to disease, and is registered to control powdery mildew by spraying in China. Seed coating is a widely applied precision method for pest prevention and control. To explore its potential in disease control and yield increase by seed coating, mixtures containing physcion and commonly used fungicides were designed and applied in a field trial. Greenhouse experiments screened the optimal concentration of physcion for seed coating an found it to be 1:50, with excellent promotion of plant growth and powdery mildew control. In field trials, seeds coated with a combinations of physcion with validamycin and pyrimidine nucleotide (2#) at 1:50 exhibited the highest emergence rate, tillering number, control effect of wheat powdery mildew, enzyme activity of ascorbate peroxidase (APX), glutathione reductase (GR), and peroxidase (POD), photosynthetic pigment content, and yield. These results provide an effective approach to wheat disease control and yield increase in wheat fields, and can lay the basis for reasonable application of physcion in Huang-huai-hai plain in China. [ABSTRACT FROM AUTHOR]

Copyright of Jurnal Tanah dan Sumberdaya Lahan is the property of Brawijaya University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Witchweed (Striga hermonthica), also called striga, is a parasitic weed that causes high yield losses in maize on more than 200 000 ha in Kenya alone. A new commercial, biological herbicide developed in Kenya is able to control striga effectively. The product was approved for use by the Pest Control Products Board in Kenya in September, 2021. It is self‐produced in villages using a secondary inoculum provided by a commercial company. The formulated product has some disadvantages, which are a complicated production process, a very short shelf life and high application rate. Additionally, the product has to be applied manually and therefore can only be used in manual production, leaving out the opportunity for farmers using mechanization. For this reason, efforts have been made to formulate the active ingredient Fusarium oxysporum f. sp. strigae strain DSM 33471, as a powder and to use it as a seed coating agent. This article deals with the production of the Fusarium spore powder, its properties, its application to the seed, and its herbicidal effect demonstrated in the first two field trials. The F. oxysporum strain was originally isolated from a wilting striga plant in Kenya. The strain was virulence enhanced to over produce the amino acids leucine, methionine and tyrosine. These amino acids are responsible for a second mode of action apart from the wilting causing effect of the fungus on striga. Whereas leucine and tyrosine have a herbicidal effect, ethylene from methionine triggers the germination of striga seeds in the soil. Additionally, the strain has been improved to be resistant to the fungicide captan, which is commonly used to treat maize seed in Kenya. Seed coating tests conducted on 25 striga‐infested small holder farms spread out in six counties of western Kenya reported yield increases of up to 88%. A second trial carried out by the Kenyan Agricultural and Livestock Research Organization showed a 93% reduction of emerged striga plants. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Seed dormancy often hinders direct seeding efforts that are attempting to restore degraded landscapes. Gibberellic acid (GA3) can be applied to physiologically dormant seeds to induce germination, but this hormone is rarely effective, as it can degrade or be leached from the seed. We tested different polymer matrixes (polylactic acid, polyvinylpyrrolidone, and ethylcellulose) to apply and slowly release GA3 to the seed. These polymers were tested as seed coatings in either a powder, liquid, or a combination of powder and liquid forms. We found that a liquid ethylcellulose/GA3 coating generally outperformed the other polymers and applications methods using our test species Penstemon palmeri. With this top-performing treatment, seed germination was 3.0- and 3.9-fold higher at 15 °C and 25 °C, respectively. We also evaluated the liquid ethylcellulose/GA3 coating on P. comharrenus, P. strictus, P. pachyphyllus, and P. eatonii. Again, the coating had a strong treatment response, with the degree of difference related to the relative level of dormancy of the species. Growth studies were also performed in pots to ensure that the side effects of GA3 overdosing were not present. Here, we found minimal differences in root length, shoot length, or biomass between plants grown from untreated and GA3-coated seeds. [ABSTRACT FROM AUTHOR]

Introduction: Salt stress in seed germination and early seedling growth is the greatest cause of crop loss in saline-alkali soils. Microbial seed coating is an effective way to promote plant growth and salt resistance, but these coatings suffer from poor seed adhesion and low survival rates under typical storage conditions. Methods: In this study, the marine bacterium Pontibacter actiniarum DSM 19842 from kelp was isolated and microencapsulated with calcium alginate using the emulsion and internal gelation method. Results: Compared to unencapsulated seeds, the spherical microcapsules demonstrated a bacterial encapsulation rate of 65.4% and survival rate increased by 22.4% at 25°C for 60 days. Under salt stress conditions, the seed germination percentage of microcapsule-embedded bacteria (M-Embed) was 90%, which was significantly increased by 17% compared to the germination percentage (73%) of no coating treatment (CK). Root growth was also significantly increased by coating with M-Embed. Chlorophyll, peroxidase, superoxide dismutase, catalase, proline, hydrogen peroxide and malondialdehyde levels indicated that the M-Embed had the best positive effects under salt stress conditions. Discussion: Therefore, embedding microorganisms in suitable capsule materials provides effective protection for the survival of the microorganism and this seed coating can alleviate salt stress in wheat. This process will benefit the development of sustainable agriculture in coastal regions with saline soils. [ABSTRACT FROM AUTHOR]

Seed coating plays a crucial role in agriculture technology as a defence mechanism for crop protection and development. Conventional seed coating methods typically involve excessive material usage, high production costs and negatively impact human health and the environment. Orthodox approaches often require the use of bulk and hazardous substances, resulting in the inefficient delivery of active ingredients. Nanotechnology has emerged as a promising alternative with its small size, high surface area, and instantaneous reactivity leading to improved efficiency and reduced material usage. Recent studies have highlighted the use of nanomaterials, specifically nanoparticles and nanofibers which offer significant benefits in boosting the seed mechanical properties, germination and vigor index by enhancing seed water uptake, and nutrient absorption due to their permeability, small size and high surface area. Nanomaterials can provide better seed protection against biotic and abiotic stresses, including pests, diseases, and environmental factors such as drought and salinity. The controlled release of active ingredients from nanomaterials enhances plant development by ensuring the seeds receive the necessary nutrients over an extended period. Nanomaterials impregnated with biochemical agents, such as hormones and enzymes, can enhance the viability of these agents and improve crop growth by enabling a systematic release mechanism. This review provides an overview of the latest developments and understanding of how nanomaterials can be applied for seed coating purposes, including their mechanism of action and potential benefits. It is expected to provide valuable insights for researchers and practitioners in the field of agriculture and contribute to the development of sustainability. [ABSTRACT FROM AUTHOR]

Ebony is one of slow-growing spe- cies), that of suspected to be the triggering factors to prone to extinction. In addition, the nature of ebony seeds is recalcitrant, so they cannot be stored for a long time. Other factors affecting seed germination are the degree of maturity, size, and weight of the seeds. Seeds harvested before the physiological maturity level would not have high viability and even could not germinate well due to the in-sufficient of food reserves, and un-perfect embryo formatio. Bio-fertilizer is used to help accelerate the growth of ebony. This study aims to analyze the combination of bio fertilizers which would be giving a better effect on the physiological activity and growth of ebony seedlings. Morphological and physiological variables measurement were used in this study. The research design used a Completely Randomized Design at a real level of 5%. If the fingerprint results obtained have a real effect, then a further test of the Duncan Multiple Range Test is carried out. Re- search results showed that seed coating treatment of 45% Penicillium microbial solution + seed coating) gave the best results in each variable of both growth and physiology of ebony seedlings, while in the treatment without seed coating, Sargassum microbial solution 30% indicates good results in each variable of growth and physiology of ebony seedlings. Treatment with the immersion of seeds in microbes can produce a high percentage in each variable.

This review addresses the issue of replacing manufactured microplastics in seed coatings used in agriculture. Firstly, it focuses on the policy and regulatory actions taken on microplastics at a global level. There is no consensus within the scientific community on the definition of a microplastic and, more generally, on the classification of plastic debris. Nevertheless, several decision schemes have been proposed in an attempt to define the notion of microplastics. The different criteria relevant to this definition, such as the size, physical state, chemical structure, origin, and persistence of microplastics, are discussed, with a comparison being made between the REACH regulation and the scientific literature. Seed production and processing are also discussed, with the functions of seed coatings being explained in order to gain a better understanding of the properties to be considered in a substitution strategy for currently used microplastics. The main challenges are multiple; substitutes must provide the same performance as microplastics: (i) improving the adherence of the treatment to the seed, (ii) distributing the treatment more evenly over the seed, (iii) reducing the amount of dust-off when handling treated seed, and (iv) improving the seed flowability, which is particularly important during the sowing stage, all while preserving the physiological properties of the seed. Substitute polymers are proposed according to the desired performance and functional properties: two main chemical families of biopolymers were identified in the literature: polysaccharides and proteins. Among them, 13 and 6 polymers, respectively, complied with REACH regulation, demonstrating adhesion, dust reduction performances, and preservation of seed physiological quality in particular. This work aims to guide future studies on microplastic substitution in seed coatings, and to highlight research needs in this area. It is based on an analysis and discussion of the literature, identifying and listing potential substitutes.

Pyrolyzed organic matter, commonly referred to as biochar, generally has positive effects on plant growth and can enhance early seedling development. Biochar-based seed coatings may thus be beneficial, specifically under adverse soil conditions such as high soil salinity. Using a series of lab and greenhouse experiments, we evaluated germination and early plant development in radish (Raphinus sativa) seeds coated with conifer-feedstock slow-pyrolysis biochar. Following the precedent of prior studies, polyvinyl acetate (PVAc) was used as the binding agent. Biochar-based seed coatings reduced germination rates and radicle extension in Petri dish trials; however, leaching of coated seeds largely eliminated this effect. PVAc applied in solution inhibited seed germination to a similar extent, suggesting that toxicity of PVAc or chemical products derived from it is the main mechanism for germination inhibition. A meta-analysis of published literature also indicates negative effects of biochar-based seed coatings using PVAc. In greenhouse trials, the same biochar seed coatings improved aspects of plant growth and physiological status relative to controls, specifically enhancing root development. We conclude that biochar-based seed coatings have promise in promoting early seedling growth and potentially in combating salinity stress in agronomic and restoration applications. However, our findings contraindicate PVAc use as a binding agent. Further research is needed to test non-phytotoxic, readily biodegradable binding agents and to optimize biochars and seed coating processes for specific applications in agronomy and ecological restoration.

Pre-sowing inoculation of agricultural crops increases the parameters that affect the yield and quality of the crop, which has been proven by many Ukrainian and foreign researchers. The purpose of the study was to set the operating parameters of the injectors at different pressures, which allowed calculating the rate of discharge of the working solution during sowing to create a system of pre-sowing inoculation, which will simplify the process of seed treatment and increase the energy efficiency of farms. Methods of system and structural analysis, mathematical statistics, abstraction, and mathematical modelling based on the Euler-Lagrange equation, and using the foundations of theoretical mechanics, physics and machine theory were used for experimental research. The planning of the experiment met the current standards, and computer graphics systems and Microsoft Excel software suite were used for statistical data processing. Based on the conducted studies, the amount of liquid that one nozzle can pass in 1 min at pressures of 300, 400, and 500 kPA was determined. It was found that the lowest result was shown by a nozzle with a diameter of 0.1 mm with a pressure of 300 kPA – 10.493 ml/min, and the highest – a nozzle with a diameter of 0.8 mm and a pressure of 500 kPA – 146.379 ml/min. At the same time, injectors with a diameter of 0.4-0.6 mm used almost the same amount of liquid at pressures of 300 and 500 kPA. The amount of liquid that can be poured per 1 ha using a six-row seeding machine with a working width of 4.2 m per sowing unit of corn was calculated. The obtained data can be used to improve any systems that use similar nozzles, such as calculating the rate of discharge of agricultural sprayers or systems that allow cooling pig farms during the summer period

Part of the dry land in Madura developed from parent material, namely sand sediments which have a low organic matter content. This study aimed to determine differences in the effectiveness of corn seed coating materials from bentonite, biochar, lignite, and biochar compost on the growth and yield of maize (Zea mays L.) under drought stress conditions. This study was conducted at the Integrated Laboratory and screen house of the Faculty of Agriculture, University of Jember, using a completely randomized design. The treatments tested in this study comprised two factors. The first factor was the treatment of coating materials, namely C0 = without coating (control), C1 = bentonite coating, C2 = biochar coating, C3 = lignite coal coating, and C4 = biochar compost coating. The second factor is the level of drought stress, namely K0 = control (100% KL moisture), K1 = Light stress (80% KL moisture), K2 = moderate stress (60% KL moisture), and K3 = severe stress (40% KL moisture). The results showed that the combination of seed coating agent treatment and drought stress had a significant effect on the parameters of plant height, number of leaves, leaf area, age of male and female flowers, cob length, cob diameter without husks, biomass dry weight, and production potential per hectare. The coal coating material and light drought stress 80% KL (C3K1) was the best combination of seed coating and drought stress treatment.