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This research project was aimed at monitoring the quality of drinking water that is supplied to the inhabitants of small villages. The quality of drinking water produced by small waterworks in South Bohemia, in municipalities Mazelov, Ortvínovice, Doubravka and Rábín, was studied. Sanitary analyses of drinking water samples carried out by the waterworks operators in 2004-2009 showed some variability in the concentrations of free chlorine, nitrates, pH, turbidity and the content of Escherichia coli in all the waterworks during the investigated period. The hypothesis assuming that the quality of drinking water produced by water treatment from small water sources is stable and that it does not vary in some key indicators, such as nitrates, the contents of Escherichia coli etc., throughout the year was not confirmed. The other hypothesis assuming that the number of small water sources used for public drinking water supplies decreases during the period was confirmed. The causes of these changes depend on many factors, such as the location and source of drinking water, the type of treatment plant, and, last but not least, the quality of service and economic potential of the waterworks operators play a negative role.