1. Prolyl carboxypeptidase mRNA expression in the mouse brain
- Author
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Sabrina Diano, Jin Kwon Jeong, Jeong, Jin Kwon, and Diano, Sabrina
- Subjects
Male ,third ventricle ,cerebral aqueduct ,ECu ,PV ,lateral septal nucleu ,Carboxypeptidase ,cingulate cortex ,spinal trigeminal nucleu ,ventromedial nucleus of the thalamu ,Gi ,Prolyl carboxypeptidase ,Cing Ctx ,medial preoptic nucleu ,Mice ,external cuneate nucleu ,dorsomedial hypothalamic nucleu ,HP ,medial habenular nucleu ,reticulotegmental nucleus of the pon ,Gene expression ,LSN ,melanocortin 3 receptor ,Xi ,Brain ,amygdala ,nucleus of solitarius tract ,hypothalamu ,Melanocortin 3 receptor ,fourth ventricle ,Cell biology ,Melanocortin 4 receptor ,substantia nigra ,external capsule ,paraventricular nucleus of the thalamu ,Melanocortin ,paraventricular nucleus of hypothalamu ,medial vestibular nucleu ,RtTg ,ventral tegmental area ,pontine reticular nucleu ,Article ,Amyg ,DMH ,PRCP: CNS ,MVe ,dorsal motor nucleus of the vagu ,D3v ,RNA, Messenger ,Molecular Biology ,DMV ,Pn ,hippocampu ,Animal ,MC3R ,PnO ,Pr ,ac ,ventrotegmental area ,Mice, Inbred C57BL ,melanocortin 4 receptor ,xiphoid thalamic nucleu ,prepositus nucleu ,Neurology (clinical) ,PVN ,ZI ,Developmental Biology ,Central Nervous System ,LH ,Indoles ,zona incerta ,MPO ,ventromedial nucleus of the hypothalamu ,lateral ventricle ,Galactoside ,Gene Expression ,DG ,anterior commissure ,Carboxypeptidases ,fornix ,Distribution ,MC4R ,Cb ,Pir ctx ,piriform cortex ,LVe ,α–MSH: alpha-melanocyte stimulating hormone ,MHb ,dorsal third ventricle ,SO ,SN ,lateral vestibular nucleu ,CA ,Arc (protein) ,General Neuroscience ,BLA ,ARC ,lateral preoptic area ,Thal ,Biochemistry ,VTA ,NTS ,cerebellum ,VM ,LPO ,supraoptic nucleu ,Mice, Transgenic ,In situ hybridization ,Sp5 ,Biology ,4v ,HYP ,arcuate nucleus of the hypothalamu ,Animals ,mammillary body ,Thalamu ,Serine protease ,ec ,lateral hypothalamu ,dentate gyru ,Galactosides ,3v ,medial preoptic area ,MM ,VMH ,gigantocellular reticular nucleu ,Indole ,Lv ,biology.protein ,pontine nuclei ,basolateral amygdala - Abstract
Prolyl carboxypeptidase (PRCP), a serine protease, is widely expressed in the body including liver, lung, kidney and brain, with a variety of known substrates such as plasma prekallikrein, bradykinin, angiotensins II and III, and α-MSH, suggesting its role in the processing of tissue-specific substrates. In the brain, PRCP has been shown to inactivate hypothalamic α-MSH, thus modulating melanocortin signaling in the control of energy metabolism. While its expression pattern has been reported in the hypothalamus, little is known on the distribution of PRCP throughout the mouse brain. This study was undertaken to determine PRCP expression in the mouse brain. Radioactive in situ hybridization was performed to determine endogenous PRCP mRNA expression. In addition, using a gene-trap mouse model for PRCP deletion, X-gal staining was performed to further determine PRCP distribution. Results from both approaches showed that PRCP gene is broadly expressed in the brain.
- Published
- 2014