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M70 M70 Sa namerom definisanja hidrogeološke uloge i značaja mikrobiološkog diverziteta odabranih pojava mineralnih voda Srbije, primenom sistema biodetektora, dokazano je prisustvo gvožđevitih, sulfato-redukujućih, sluz-produkujućih, heterotrofnih aerobnih i denitrifikacionih bakterija, na osnovu čega je proračunat rizik od razvoja procesa biokorozije i biohemijske inkrustacije, te zdravstveni rizik. Uz procenu rizika, izvedene su analize skenirajuće elektronske mikroskopije biofilmova i hidrogeohemijsko modeliranje, te makroskopska, otpička i difrakciona ispitivanja naslaga inkrustacije, dok je fizičko-hemijskim analizama utvrđena vrednost ukupne mineralizacije iznad 1 g/L, kao i povišeni sadržaji odgovarajućih metala i nemetala. Metabarkoding analizama mikrobioma mineralnih voda dokazano je prisustvo razdela Proteobacteria, Bacteroidetes, Patescibacteria, Chlorflexi, Firmicutes, Spirochaetes i Acidobacteria u većem procentu. Pseudomonas, Limnobacter i Rheinheimera su bili najzastupljeniji rodovi u uzorku mineralnih voda Torde, Acinetobacter i Massilia u uzorku iz Obrenovačke Banje, Maritimimonas, Phreatobacter, Planktosalinus, Acinetobacter, Geminocystis i Sulfuritalea u uzorku iz Lomnice, Pseudorhodobacter i Limnohabitans u mineralnim vodama Velike Vrbnice, dok su u uzorku iz Slankamen Banje dominirale zelene sumporne bakterije, Methylotenera, Flavobacterium, Melioribacter i Dechloromonas. Realizovanim istraživanjima zaključeno je da odgovarajući predstavnici mikroorganizama utiču na starenje bunara i definišu ekološki status vodnih resursa, dok s druge strane pojedini rodovi poseduju biotehnološki potencijal u tretmanu remedijacije kontaminiranih voda, zbog čega postoji neophodnost implementacije biohidrogeologije u okviru standardne metodike hidrogeoloških istraživanja. The presence of iron related, sulphate reducing, slime producing, heterotrophic aerobic and denitrifying bacteria was proved applying biodetectors, in order to define the hydrogeological role and importance of the microbiological diversity of select occurrences of mineral waters in Serbia. Then the biocorrosion, biochemical encrustation and health risks were assessed. The research included scanning electron microscopy of biofilms, hydrogeochemical modelling and macroscopic, optical and diffraction examination of the encrustations. Physical and chemical analyses indicated total dissolved salts of more than 1 g/L and elevated concentrations of certain metals and non-metals. Metabarcoding of the microbiomes of the mineral waters revealed the presence of a high percentage of the Proteobacteria, Bacteroidetes, Patescibacteria, Chlorflexi, Firmicutes, Spirochaetes and Acidobacteria divisions. Pseudomonas, Limnobacter and Rheinheimera were the most representative genera in the mineral water sample from Torda, Acinetobacter and Massilia in the sample collected in Obrenovačka Banja, Maritimimonas, Phreatobacter, Planktosalinus, Acinetobacter, Geminocystis and Sulfuritalea in the sample from Lomnica, and Pseudorhodobacter and Limnohabitans in the sample from Velika Vrbnica, while in the sample from Slankamen Banja was dominated by green sulphur bacteria, Methylotenera, Flavobacterium, Melioribacter and Dechloromonas. The research concludes that the respective microorganisms cause water well aging and define the ecological status of the water resources, while, on the other hand, some of the genera possess the biotechnological potential for treating and remediating contaminated waters, which is a compelling reason for implementing biohydrogeology in the standard hydrogeological research methodology.

Tri uzorka glina: TWVA, VB i Stanari, istraživana su metodom rendgenske difrakcijske analize. U svrhu nedvojbene identifikacije minerala glina koje uzorci sadrže provedene su analize izvornih uzoraka, uzoraka termički obrađenih pri temperaturi od 350 i 550 °C te uzoraka obrađenih s etilen glikolom. Utvrđeno je da se uzorak TWVA sastoji od kvarca, kaolinita i muskovita; uzorak VB od kvarca, kristobalita, montmorilonita i glinenca te uzorak Stanari od kvarca, montmorilonita i kaolinita. Naime, udaljenost slojeva u kaolinitu se termičkom obradom pri 350°C ne mijenja, dok termičkom obradom pri 550°C dolazi do raspada kaolinita. Udaljenost slojeva u kaolinitu glikolacijom se ne mijenja. Zagrijavanjem montmorilonita pri 350°C i 550 °C dolazi do smanjenja udaljenosti slojeva, dok glikolacijom dolazi do povećanja udaljenosti slojeva. Three clay samples: TWVA, VB and Stanari, have been investigated via X-ray diffraction analysis. In order to make undoubted identification of clay minerals that samples are composed of, the analysis of as received samples, samples thermally treated at temperatures of 350 and 550 °C and samples treated with ethylene glycol have been conducted. It has been determined that sample TWVA is composed of quartz, kaolinite and muscovite, sample VB of quartz, montmorillonite and feldspars and sample Stanari of quartz, montmorillonite and kaolinite. Namely, the distance between kaolinite layers doesn’t change with thermal treatment at 350°C, while thermal treatment at 550°C causes the decomposition of kaolinite. Glycolation of kaolinite doesn’t affect the interlayer distance. Heating of montmorillonite at 350°C as well at 550 °C causes the reduction of interlayer distance while glycolation causes the increase of interlayer distance.

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