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AbstractThe aim of this study was to investigate the rehydration characteristics of shrimp treated by osmotic dehydration packaging (ODP). The rehydration rate and moisture distribution of dried shrimp during rehydration were evaluated. Besides, the changes in properties and structure of proteins, digestibility and microstructure of shrimp dehydrated to 60%, 45% and 30% moisture content by ODP were characterized compared with those of the hot air dried (HAD). The results showed that the rehydration rate of dried shrimps treated by ODP to different moisture contents was higher than that of the corresponding HAD. Low-field nuclear magnetic resonance analysis revealed that the relaxation times (T22 and T23) of ODP-treated shrimp increased over time, which indicated an increase in water mobility, contributing to faster and better water uptake. Furthermore, ODP-treated shrimp had less variation in actomyosin solubility, turbidity, total sulfhydryl and carbonyl content. Not only the network structure of myofibers could be preserved, but also the tertiary structure of the actomyosin was more stable. These findings emphasized the effectiveness of low-temperature ODP in mitigating oxidation and aggregation processes, enhancing rehydration and digestibility of the shrimp. [ABSTRACT FROM AUTHOR]

Summary Leaf architecture impacts the ease of gases diffusion, biochemical process, and photosynthetic performance. For balsam poplar, a widespread North American species, the influence of water availability on leaf anatomy and subsequent photosynthetic performance remains unknown. To address this shortcoming, we characterized the anatomical changes across the leaf profile in three‐dimensional space for saplings subjected to soil drying and rewatering using X‐ray microcomputed tomography. Our hypothesis was that higher abundance of bundle sheet extensions (BSE) minimizes drought‐induced changes in intercellular airspace volume relative to mesophyll volume (i.e. mesophyll porosity, θIAS) and aids recovery by supporting leaf structural integrity. Leaves of ‘Carnduff‐9’ with less abundant BSEs exhibited greater θIAS, higher spongy mesophyll surface area, reduced palisade mesophyll surface area, and less veins compared with ‘Gillam‐5’. Under drought conditions, Carnduff‐9 showed significant changes in θIAS across leaf profile while that was little for ‘Gillam‐5’. Under rewatered conditions, drought‐induced changes in θIAS were fully reversible in ‘Gillam‐5’ but not in ‘Carnduff‐9’. Our data suggest that a ‘robust’ leaf structure with higher abundance of BSEs, reduced θIAS, and relatively large mesophyll surface area provides for improved photosynthetic capacity under drought and supports recovery in leaf architecture after rewatering in balsam poplar. [ABSTRACT FROM AUTHOR]

AbstractIn order to reduce the drying time of broccoli and to investigate rehydration and color changes associated with the drying process, infrared drying method and blanching pretreatment were used in this study. Boccoli florets were dried using infrared drying at power levels of 50, 62, 74 and 88 W to investigate drying kinetics. Blanching was used as a pretreatment and it was found that the blanched samples dried faster. Effective moisture diffusion coefficients were also calculated. While Deff values between 9.26 × 10−11 and 2.15 × 10−10 m2/s were observed for the control samples, this value varied between 1.10 × 10−10 and 2.87 × 10−10 m2/s for the blanched samples. The calculated activation energy values for the blanched and control samples are 2.11 and 2.0 kW/kg, respectively. The drying process was modeled using 12 different thin-layer drying models and it was found that the Midilli & Kucuk model best represented the process. Color analysis was performed by measuring L, a and b values as well as chroma, hue angle and total color change values. It was observed that increasing infrared power affected color parameters, leading to a decrease in L values and an increase in a and b values. In addition, blanching pretreatment preserved color to some extent. The rehydration behavior of dried samples was also analyzed. It was found that the highest rehydration capacity was achieved in blanched samples dried at 74 W. [ABSTRACT FROM AUTHOR]

Objectives: Systemic dehydration may induce osmotic and oxidative stress in the vocal folds, but our knowledge of the biology and mitigation with rehydration is limited. The purpose of this experiment was to evaluate whether systemic dehydration induces vocal fold oxidative and osmotic stress and to compare the impact of rehydration by water intake versus electrolyte intake on osmotic and oxidative stress‐related gene expression. Methods: Four‐month‐old male Sprague–Dawley rats (N = 32) underwent water restriction. Rehydration was achieved with ad libitum access to water or electrolytes for 24 hours. Rats were divided into four groups: euhydration control, dehydration‐only, dehydration followed by either water or electrolyte rehydration (n = 8/group). Gene expression was assessed via RT2 Gene Expression Profiler arrays. Results: With respect to oxidative stress, 10 genes were upregulated and 2 were downregulated after vocal fold dehydration compared with the euhydrated control. Concerning osmotic stress, six genes were upregulated with dehydration only, six genes were upregulated following rehydration with water, whereas a single gene was upregulated with electrolyte rehydration. All genes with significantly different expression between the rehydration groups showed lower expression with electrolytes compared with water. Conclusions: The results support a potential role of oxidative and osmotic stresses in vocal folds related to systemic dehydration. The differences in stress‐related gene expression in vocal fold tissue between rehydration with electrolytes or water, albeit modest, suggest that both rehydration options offer clinical utility to subjects experiencing vocal fold dehydration with preliminary evidence that electrolytes may be more effective than water in resolving osmotic stress. Level of Evidence: NA (prospective animal study) Laryngoscope, 134:4636–4641, 2024 [ABSTRACT FROM AUTHOR]

Variable-temperature convective drying (VTCD) is a promising technology for obtaining high-quality dried mushrooms, particularly when considering rehydration capacity. However, accurate numerical models for variable-temperature convective drying and rehydration of shiitake mushrooms are lacking. This study addresses this gap by employing a model with thermo–hydro and mechanical bidirectional coupling to investigate five dehydration characteristics (moisture ratio, drying rate, temperature, evaporation rate, and volume shrinkage ratio) and a drying load characteristic (enthalpy difference) during VTCD. Additionally, a mathematical model combining drying and rehydration is proposed to analyze the effect of VTCD processes on the rehydration performance of shiitake mushrooms. The results demonstrate that, compared to constant-temperature drying, VTCD-dried mushrooms exhibit moderate shrinkage rates and drying time (16.89 h), along with reduced temperature variation and evaporation rate gradient (Max. 1.50 mol/(m3·s)). VTCD also improves enthalpy stability, reducing the maximum drying load by 58.84% compared to 338.15 K constant-temperature drying. Furthermore, drying time at medium temperatures (318.15–328.15 K) greatly influences rehydration performance. This study quantitatively highlights the superiority of variable-temperature convective drying, offering valuable insights for optimizing the shiitake mushroom drying processes. [ABSTRACT FROM AUTHOR]

Drought is the most significant abiotic stress that impedes agroforestry development. In nature, drought tolerance also depends on the ability to compensate after water restoration. Dark septate endophytes (DSEs) are believed to enhance plant tolerance in drought environments. However, the compensatory mechanisms of DSEs for rehydration after drought stress have not been reported. To assess the drought tolerance and compensatory capacity of DSEs, the following DSEs were investigated in this study using solid–liquid screening and potting tests under different drought gradients, rehydration conditions, and field water-holding capacities: Stagonosporopsis lupini, Microsphaeropsis cytisi, Macrophomina pseudophaseolina, Paraphoma radicina, Alternaria alstroemeriae, Alternaria tellustris, and Papulaspora equi. The results showed that M. pseudophaseolina reached the maximum diameter for plate growth in only 4 d. In a liquid shaker, the biomass of S. lupini peaked after rehydration. The Mantel heatmap indicated that lipid metabolites were significantly expressed in M. pseudophaseolina and S. lupini under drought stress. Correlations between drought tolerance indexes and amino acid metabolites increased dramatically in both DSEs after rehydration. Moreover, in rehydration after drought, the treatments inoculated with M. pseudophaseolina and S. lupini showed significant increases in root weight of 20.36% and 23.82%, respectively, compared with the uninoculated treatment. [ABSTRACT FROM AUTHOR]

Subduction-zone fluid–rock interactions have a direct impact onto elemental and isotopic homogeneity of progressively buried and exhumed crustal lithologies by providing an interface for local mass-transfer and enhancing metamorphic reactions. In order to assess the scales of fluid mobility, chemical and isotopic inheritance, as well as resulting degrees of isotopic heterogeneity in the exhumed high-pressure lithologies, we performed the detailed mineralogical, in-situ trace-element and Rb–Sr isotope studies, combined with P–T–X thermodynamic modelling of representative eclogites from the Alag Khadny accretionary complex (SW Mongolia). The eclogites (garnet + omphacite + phengite + rutile + quartz + retrograde amphibole and clinozoisite) display records of subduction-related burial to 540–625 °C and 1.7–2.1 GPa, with the enclosed phengite supposed to be in equilibrium at prograde-to-peak conditions. Trace-element signatures, including Cs/Rb (0.03–0.08) and Ba/Rb (7.1–13.8) ratios of phengite, are consistent with moderately to strongly altered protoliths of eclogites, which is supported by elevated δ18O values and in-situ Rb–Sr constraints on the initial (87Sr/86Sr)I ratios of phengite within 0.70549–0.70957. Partial backward rehydration (~ 0.5–1.0 wt% of H2O added) during decompression from 1.6 to 1.2 GPa produced amphibole- and clinozoisite-bearing assemblages, did not significantly affect LILE systematics and variable 87Rb/86Sr ratios of phengite. Limited Rb and Ba loss from phengite during recrystallization is suspected in the evidently deformed eclogites based on the LILE mineral–fluid and phengite–amphibole partitioning data. No exclusive evidence is found in amphibole for LILE-rich metasedimentary fluid with high (87Sr/86Sr)I released into eclogites. Instead, unradiogenic 87Sr/86Sr (0.70279–0.70301) of clinozoisite highlights metasomatic addition from the underlying mafic crust or dehydrated peridotitic mantle. Variable deformation-enhanced fluid-rock interaction during early exhumation was recorded by in-situ phengite Rb-Sr geochronology at 568 ± 9 Ma, which is considered a direct fluid flow snapshot and place a new minimum age constraint for the peak subduction burial. We argue that, except cases of apparent metasomatic origin of phengite, its (87Sr/86Sr)I ratios may be a sensitive tracer for the eclogite precursor alteration due to limited Sr mobility. Sample-scale Rb-Sr isotopic heterogeneities may be preserved in the orogenic eclogites due to multi-stage retrograde hydration and should be taken into account while interpreting the bulk-rock Sr isotope data. [ABSTRACT FROM AUTHOR]

A solar-electrical hybrid dryer (SEHD) of 10 kg capacity was designed and developed for drying of fishery products under controlled and hygienic conditions. SEHD consisted of a drying chamber with total drying area of 3.06 m2, solar air collector, electric heating coil, blower, exhaust fans, temperature sensor, and control panels. Fabrication of dryer was done using stainless steel (SS 304) for cabinet and drying trays, galvanized iron for the frame, MS angle frame for support, polyurethane foam and aluminum foil for insulation of drying chamber, and glazing and fiber boards for outer covering and structure. SEHD is designed to utilize solar energy for drying and is supplemented with 750 W electric heating coil to operate during off-sunshine hours. The shrimp moisture content reduced from 80.05% (w.b.) to 14.38% (w.b.) in 6 h in SEHD. The maximum collector temperature obtained during the experiment was 64.4°C with an average incident radiation of 710 W/m2. The collector efficiency was found to be 37.39%. The drying efficiency of 28.65% was observed in SEHD. The exergy efficiency of the drying chamber and solar air collector was estimated to be 28.57 and 62.50%, respectively. The energy payback period of the designed SEHD was found to be 0.58 years, with a sustainability index of 1.4. The economic payback period was estimated to be 0.73 years. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

AbstractStarch-based foods have a large consumer base worldwide. It is known that the quality of freeze-dried starch-based products is subject to deterioration during the production process. The present study was unprecedented carried out to examine the effects of freeze-drying on the cross-linking between components of gelatinized starch-based system. The results showed that the retrogradation of starch in the process of dehydration, the weakening of the binding capacity between the substrate and water of the freeze-dried product and the weakening of the interaction strength between the components of the product were the key mechanisms leading to the quality deterioration of the wheat starch-based conditioned food during the freeze-drying. The addition of lipid and protein can inhibit starch retrogradation during freeze-drying, and the inhibition effect of oil is better. However, the intervention of oil and fat will cause dehydrated starch to re-adsorb the melted oil/water mixture during the freeze-drying process, resulting in the deterioration of product quality. The intervention of protein will improve the binding ability of freeze-dried wheat starch-based product matrix to water. In the ternary system, the intervention of protein will weaken the inhibition effect of lipid on retrogradation of starch in the freeze-drying process. In addition, the lower drying temperature will lead to the decrease of the interaction strength between the components of ternary mixed system. Avoiding the melting of frozen materials during drying is the key control point to improve the quality of freeze-dried wheat starch-based products. [ABSTRACT FROM AUTHOR]

Summary: This study investigates the reversible and irreversible structural changes in proteins induced by different salting methods (brining, mixed salting, and dry salting) during the desalting process of Merluccius hubbsi fillets. We focused on mass changes, water content, and NaCl content, employing mathematical models for kinetic analysis and sorption isotherms. Image analysis was utilised to examine tissue microstructure and texture profile analysis assessed the mechanical properties. Our results indicate that salting methods significantly influence mass changes and yield during desalting. Specifically, brining led to the highest weight gain due to controlled salt absorption and enhanced water retention. The Peleg model accurately described NaCl and water content dynamics during desalting. Microscopic analysis revealed substantial structural alterations, while mechanical properties showed significant differences; dry and mixed salting methods caused notable tissue damage and protein denaturation. These findings provide valuable insights for enhancing salting processes and improving the quality of desalted seafood products. [ABSTRACT FROM AUTHOR]

To assess the regulatory mechanisms involved in the transcriptomic response of Ginkgo biloba to water shock and rehydration, ginkgo seedlings were subjected to dehydration for 0, 3, 6, 12, and 24 h, followed by rehydration for 12 h (Re12 h). A total of 1388, 1802, 2267, 2667, and 3352 genes were upregulated, whereas 1604, 1839, 1934, 2435, and 3035 genes were downregulated, at 3, 6, 12, 24, and Re12 h, respectively, compared to 0 h. Two KEGG pathways—the plant pathogen interaction pathway and mitogen-activated protein kinase (MAPK) signaling pathway—were enriched under water shock but not under rehydration. Moreover, plant hormone signal transduction was enriched under both water shock and rehydration. Differentially expressed genes (DEGs) involved in the ABA signaling pathway (PYR/PYLs, PP2Cs, and SnRK2s) and major differentially expressed transcription factors (MYB, bHLH, AP2/ERF, NAC, WRKY, and bZIP TFs) were identified. qRT-PCR analysis further revealed GbWRKY3 as a negative regulator of the water shock response in G. biloba. The subcellular localization results revealed GbWRKY3 as a nuclear protein. These phenotype-related DEGs, pathways, and TFs provide valuable insight into the water shock and rehydration response in G. biloba. [ABSTRACT FROM AUTHOR]

A key element in the effective recovery of plants after drought is slowing down of their senescence accelerated during the drought. Therefore, in this paper the relationships between hydrogen peroxide content and effective recovery during rehydration after water stress were analyzed. The study focused on two DH lines of winter triticale with different regeneration potential, as shown by different numbers of lateral stems with ears, grown during rehydration after water stress. During rehydration, the GZDH27 line grows two to three lateral stems with ears, whereas the GZDH88 line grows a single lateral stem with ear. The two DH lines of winter triticale showed comparable responses to the drought stress but their responses to rehydration after the drought stress were clearly different. Results revealed, that the increased accumulation of hydrogen peroxide, which was induced during the drought, can be maintained during rehydration, thus stimulating plant senescence. In the line GZDH88, rehydration did not slow down drought-induced senescence. This was manifested by higher levels of hydrogen peroxide, accompanied by lower levels of chlorophyll in the flag leaves of GZDH88 plants, as the result of which only one lateral stem was formed during rehydration. However, development of one lateral stems during rehydration in the GZDH88 line allowed for smaller yield loss than in the GZDH77 line, that developed of three lateral stems during rehydration. The efficient production of carbohydrates and their effective allocation into the ears of the lateral stems required considerable energy expenditure in the GZDH27 line. Our results indicate that growing three lateral stems with ears during rehydration does not guarantee limitation of grain yield loss. However, to reach the final conclusion, it is important to evaluate a greater number of triticale genotypes under drought stress and rehydration during various stages of a plants development. [ABSTRACT FROM AUTHOR]

Fresh shiitake mushroom is rich in various nutrients such as carbohydrates, proteins, fats, vitamins and minerals, with antioxidant, anti-tumor, antibacterial and immunoenhancing effects. Due to its short storage life and susceptibility to deterioration, fresh shiitake mushroom is usually processed into dried products, which can effectively extend the storage period. The Maillard reaction during the drying process can provide a special flavor to shiitake mushroom, and the rehydration characteristics of dried shiitake mushroom are important factors determining its quality. This paper introduces the drying and rehydration processes of shiitake mushroom and the advantages and disadvantages of different rehydration methods, and reviews the characteristics of different rehydration methods, the factors affecting the rehydration process and the influence of rehydration on the quality of shiitake mushroom as well as recent progress on the modeling of rehydration kinetics. In addition, it deeply discusses the changes of texture, flavor, color and cell structure during the rehydration process of shiitake mushroom. The aim of this review is to provide new ideas and references for research on shiitake mushroom rehydration and processing.

BACKGROUND: The effect of dehydration of ex vivo cartilage samples and rehydration with native synovial fluid or normal saline on quantitative ultrashort echo time (UTE) biomarkers are unknown. We aimed to investigate the effect of cartilage dehydration-rehydration on UTE biomarkers and to compare the rehydration capabilities of native synovial fluid and normal saline. METHODS: A total of 37 cartilage samples were harvested from patients (n=5) who underwent total knee replacement. Fresh cartilage samples were exposed to air to dehydrate for 2 hours after baseline magnetic resonance (MR) scanning, then randomly divided into two groups: one soaking in native synovial fluid (n=17) and the other in normal saline (n=20) to rehydrate for 4 hours. UTE-based biomarkers [T1, adiabatic T1r (AdiabT1r), macromolecular fraction (MMF), magnetization transfer ratio (MTR), and T2*] and sample weights were evaluated for fresh, dehydrated, and rehydrated cartilage samples. Differences and agreements between groups were assessed using the values of fresh cartilage samples as reference standard. RESULTS: Dehydrating in air for 2 hours resulted in significant weight loss (P=0.000). T1, AdiabT1r, and T2* decreased significantly while MMF and MTR increased significantly (all P0.0167, with Bonferroni correction) while T2* (P=0.001) still had significant differences compared with fresh samples. However, no significant differences were detected for any of the evaluated UTE biomarkers after rehydrating in normal saline (all P>0.05). No differences were detected in the agreement of UTE biomarker measurements between fresh samples and samples rehydrated with synovial fluid and normal saline. CONCLUSIONS: Cartilage dehydration resulted in significant changes in UTE biomarkers. Rehydrating with synovial fluid or normal saline had non-significant effect on all the evaluated UTE biomarkers except T2* values, which still had significant differences compared with fresh samples after rehydrating with synovial fluid. No significant difference was observed in the rehydration capabilities of native synovial fluid and normal saline.

Hydration status refers to the balance between the intake and discharge of water in the body. When the ingested and discharged water are roughly equal and the body is in water balance, it is the normal hydration status, and when the water intake is too little or too much, it is the "dehydration" or "overhydration status". The hydration status of the body not only affects metabolism, but also affects the functions of the urinary system, cardiovascular system, nervous system, etc. In order to further clarify the relationship between body hydration status and decompression sickness (DCS), this paper reviewed relevant studies and analyzed the interaction between hydration and decompression safety during diving. The primary causes of dehydration in diving are "hyperbaric diuresis", "immersion diuresis", breathing dry gas, heat, and cold. Dehydration not only promotes the occurrence of DCS but also reduces the aerobic work efficiency and athletic performance of divers, as well as affects cognition and mood. A study found that appropriate rehydration before and during diving can reduce the risk of DCS, which possibly associates with the increase of blood volume, plasma surface tension, and vasoconstriction. Fluid therapy is also important for those who already have DCS. This paper analyzed the amount, nature, timing, and effect of rehydration involved in the above links, comprehensively sorted out the relationship between hydration and diving safety, summarized the existing problems, and provided reference for practical application and future research.

Abstract The growth, yield, and seed quality of rapeseed are negatively affected by drought stress. Therefore, it is of great value to understand the molecular mechanism behind this phenomenon. In a previous study, long non-coding RNAs (lncRNAs) were found to play a key role in the response of rapeseed seedlings to drought stress. However, many questions remained unanswered. This study was the first to investigate the expression profile of lncRNAs not only under control and drought treatment, but also under the rehydration treatment. A total of 381 differentially expressed lncRNA and 10,253 differentially expressed mRNAs were identified in the comparison between drought stress and control condition. In the transition from drought stress to rehydration, 477 differentially expressed lncRNAs and 12,543 differentially expressed mRNAs were detected. After identifying the differentially expressed (DE) lncRNAs, the comprehensive lncRNAs-engaged network with the co-expressed mRNAs in leaves under control, drought and rehydration was investigated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of co-expressed mRNAs identified the most significant pathways related with plant hormones (expecially abscisic acid, auxin, cytokinins, and gibberellins) in the signal transduction. The genes, co-expressed with the most-enriched DE-lncRNAs, were considered as the most effective candidates in the water-loss and water-recovery processes, including protein phosphatase 2 C (PP2C), ABRE-binding factors (ABFs), and SMALL AUXIN UP-REGULATED RNAs (SAURs). In summary, these analyses clearly demonstrated that DE-lncRNAs can act as a regulatory hub in plant-water interaction by controlling phytohormone signaling pathways and provided an alternative way to explore the complex mechanisms of drought tolerance in rapeseed.

Neurostimulation/neurorecording are tools to study, diagnose, and treat neurological/psychiatric conditions. Both techniques depend on volume conduction between scalp and excitable brain tissue. Here, we examine how neurostimulation with transcranial magnetic stimulation (TMS) is affected by hydration status, a physiological variable that can influence the volume of fluid spaces/cells, excitability, and cellular/global brain functioning. Normal healthy adult participants (32, 9 males) had common motor TMS measures taken in a repeated-measures design from dehydrated (12-h overnight fast/thirst) and rehydrated (identical dehydration protocol followed by rehydration with 1 L water in 1 h) testing days. The target region was left primary motor cortex hand area. Response at the target muscle was recorded with electromyography. Urinalysis confirmed hydration status. Motor hotspot shifted in half of participants. Motor threshold decreased in rehydration, indicating increased excitability. Even after redosing/relocalizing TMS to the new threshold/hotspot, rehydration still showed evidence of increased excitability: recruitment curve measures generally shifted upward and the glutamate-dependent paired-pulse protocol, short intracortical facilitation (SICF), was increased. Short intracortical inhibition (SICI), long intracortical inhibition (LICI), long intracortical facilitation (LICF), and cortical silent period (CSP) were relatively unaffected. The hydration perturbations were mild/subclinical based on the magnitude/speed and urinalysis. Motor TMS measures showed evidence of expected physiological changes of osmotic challenges. Rehydration showed signs of macroscopic and microscopic volume changes including decreased scalp-cortex distance (brain closer to stimulator) and astrocyte swelling-induced glutamate release. Hydration may be a source of variability affecting any techniques dependent on brain volumes/volume conduction. These concepts are important for researchers/clinicians using such techniques or dealing with the wide variety of disease processes involving water balance. NEW & NOTEWORTHY: Hydration status can affect brain volumes and excitability, which should affect techniques dependent on electrical volume conduction, including neurostimulation/recording. We test the previously unknown effects of hydration on neurostimulation with TMS and briefly review relevant physiology of hydration. Rehydration showed lower motor threshold, shifted motor hotspot, and generally larger responses even after compensating for threshold/hotspot changes. This is important for clinical and research applications of neurostimulation/neurorecording and the many clinical disorders related to water balance. [ABSTRACT FROM AUTHOR]

Background: Considering the high amount of water in watermelon, this study aimed to investigate the effect of watermelon juice compared to carbohydrate drinks and water on rehydration and anaerobic performance. Methods: In this quasi-experimental study, twelve active adolescents experienced weight loss of about 2% in three separate sessions, one week apart, by running a treadmill exercise program including two sets of 30-minute aerobic activity separated by a 10-minute rest. Then, they consumed one of the three drinks of water, carbohydrate drink, or watermelon juice at 125% of their weight loss. Two hours after each session, they performed a 300-yard shuttle run test. Urine specific gravity (USG) and participants’ weight were measured three times: before and after the dehydration protocol and before the anaerobic performance. Results: Data analysis showed no significant difference in USG (P=0.41) and the 300-yard shuttle run test (P=0.87) after consuming the three drinks. There was a significant difference (P=0.001) in weight gain after consuming the three drinks, with greater mean weight gain for watermelon juice and carbohydrate drinks compared to water. Conclusion: The results indicated the same effect in carbohydrate drinks and watermelon juice on weight return, rehydration, and subsequent anaerobic performance. However, because watermelon juice contains vitamins and minerals compared with carbohydrate drinks, it is suggested that athletes use this drink. [ABSTRACT FROM AUTHOR]

As a renewable, biocompatible, biodegradable soft material, chitin hydrogels have better advantages in stability, antibacterial activity, antifouling, cost, immunogenicity, and so on than most polymer hydrogels. However, compared with other widely used polymer hydrogels with high strength and toughness, the practical applications of chitin-based hydrogels have been limited by their weak mechanical properties, such as cartilage repair and meniscus replacement. Here, we present the design and fabrication of chitin hydrogels with excellent mechanical strength and toughness by a dehydration and rehydration strategy. By sequential dehydration and rehydration processes, the crystalline domains in the chitin hydrogels can be properly controlled. With optimized crystallinity, the elastic modulus of the chitin hydrogels exceeds all previously reported values, and the fracture toughness is even comparable to some synthetic polymer hydrogels, while maintaining a high-water-content of about 80 wt.%. At the same water content, the mechanical properties of the chitin hydrogels are positively correlated with the hydrogel crystallinity, which proves that the change of mechanical properties of hydrogels is not simply dependent on weight concentration. The hydrogels can be further strengthened by incorporating other biopolymers that are intrinsically weak, which makes the hydrogels promising for applications in fields such as cartilage repair and meniscus replacement. Moreover, the hydrogels enable loading and release of water-soluble and poorly water-soluble drugs. This highly extendable strengthening and toughening strategy of chitin and chitin-based biopolymer hydrogels paves the way for their widely applications. [ABSTRACT FROM AUTHOR]

Copyright of Food & Fermentation Industries is the property of Food & Fermentation Industries and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The growth, yield, and seed quality of rapeseed are negatively affected by drought stress. Therefore, it is of great value to understand the molecular mechanism behind this phenomenon. In a previous study, long non-coding RNAs (lncRNAs) were found to play a key role in the response of rapeseed seedlings to drought stress. However, many questions remained unanswered. This study was the first to investigate the expression profile of lncRNAs not only under control and drought treatment, but also under the rehydration treatment. A total of 381 differentially expressed lncRNA and 10,253 differentially expressed mRNAs were identified in the comparison between drought stress and control condition. In the transition from drought stress to rehydration, 477 differentially expressed lncRNAs and 12,543 differentially expressed mRNAs were detected. After identifying the differentially expressed (DE) lncRNAs, the comprehensive lncRNAs-engaged network with the co-expressed mRNAs in leaves under control, drought and rehydration was investigated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of co-expressed mRNAs identified the most significant pathways related with plant hormones (expecially abscisic acid, auxin, cytokinins, and gibberellins) in the signal transduction. The genes, co-expressed with the most-enriched DE-lncRNAs, were considered as the most effective candidates in the water-loss and water-recovery processes, including protein phosphatase 2 C (PP2C), ABRE-binding factors (ABFs), and SMALL AUXIN UP-REGULATED RNAs (SAURs). In summary, these analyses clearly demonstrated that DE-lncRNAs can act as a regulatory hub in plant-water interaction by controlling phytohormone signaling pathways and provided an alternative way to explore the complex mechanisms of drought tolerance in rapeseed. [ABSTRACT FROM AUTHOR]

Rehydration of food products was carried out in various amounts of liquid. The effects of the solid-to-liquid ratio on rehydration characteristics of dried red beets were investigated. Six values of the solid-to-liquid ratio (1:20, 1:15, 1:10, 1:5, 1:3, and 1:1) were taken into consideration. Red beets cubes (10 mm) were dried in a forced convection (2 m/s; 60 °C) and then rehydrated in distilled water at a temperature of 20 °C. The kinetics of mass gain, dry matter loss, water gain, moisture content increase, and volume increase were modeled using the empirical Peleg model which parameters have physical meanings. Equations were formulated to make the Peleg model parameters dependent on the solid-to-liquid ratio. The obtained results indicated that the amount of rehydrating water influenced the values of the model parameters and the course of dried red beets rehydration and helped explain the rehydration kinetics. The Peleg model can be recommended for describing the kinetics of mass gain (R2 = 0.9662–0.9895), dry matter loss (R2 = 0.7042–0.9881), water gain (R2 = 0.9732–0.9913), moisture content increase (R2 = 0.9770–0.9929), and volume increase (R2 = 0.9374–0.9847) during the rehydration of dried red beet cubes at all considered values of the solid-to-liquid ratio. [ABSTRACT FROM AUTHOR]

Introduction: Hydrates are often used as pharmaceutical active pharmaceutical ingredients (API), especially when anhydrates may not be feasible likely due to physicochemical properties concerns. Pharmaceutical hydrates, whereas water is present as crystal adduct, are feasible for drug products as they do not pose any safety concern. Hydrates can impart many different advantages; therefore, they are quite common and preferred solid forms for numerous pharmaceutical materials on market. However, hydrates may involve various phase transitions, which may impact the stability and processability of drug substance. Methods: Phase transitions, which include temperature-induced dehydration and moisture-facilitated rehydration are investigated by different solid-state analytical techniques such as powder x-ray diffraction, thermogravimetric analysis, differential scanning calorimetry, polarized light microscopy, and single-crystal x-ray diffraction. Results: This research investigation focuses on the different phase transition behaviors of a newly discovered pharmaceutical compound with three channel hydrates, two of which confirmed by single-crystal analysis. The retention or rearrangement of crystal structures over the transitions are studied. Hydrate 3 exhibits a characteristic feature of channel hydrate that involves symmetric lattice relaxation. Unlike hydrate 3, hydrate 2 results in a potentially new unit cell upon dehydration due to asymmetric lattice relaxation, which converted back to Hydrate 2 in presence of water, a very unique behavior for a channel hydrate, rarely observed, which entails novelty of this research work. Conclusion: The relationship among crystal forms of different hydrates of this new compound is thus established. The current investigation is a vital part of drug product risk assessment for hydrates to avoid any challenges during manufacturing operations and/or stability studies. This investigation was successfully applied in the present study and can be expanded to other newly discovered APIs in future. [ABSTRACT FROM AUTHOR]

Researchers have observed that rainfall may re‐initiate decomposition in desiccated tissue; however, no conclusive research‐based evidence exists on the specific effects of rehydration on decomposition. Therefore, this study aimed to assess the effects of artificial rehydration on the progression of decomposition following the advanced stage of decomposition. Twelve adult pig cadavers (8 experimental; 4 controls) were placed in the central Highveld of South Africa during cooler (April–July 2021) and warmer (August–November 2021) months. Decomposition was scored approximately biweekly to obtain the total body score, and accumulated degree days (ADD) were calculated for each pig. All pig cadavers were covered by chicken wire cages with transparent tarps to control for natural rehydration and scavenging. Once the experimental pig cadavers reached a three‐visit stasis in the advanced phase of decomposition, they were artificially rehydrated, and changes in the progression of decomposition between the control and experimental groups were plotted (ADD against TBS) for observation. The rehydrated experimental pig cadavers showed re‐initiation of decay and insect re‐colonization, while the control cadavers mainly remained in a state of stasis with insect activity ceased altogether. Greater cadaver decomposition islands and a color change post‐rehydration were also noted in some experimental cadavers. This supports the need for future research on the impact of rehydration, including associated soil moisture on decomposition rates, progression, and invertebrate colonization, which will enhance our understanding of the effects these environmental factors have on the accuracy of post‐mortem interval estimation. [ABSTRACT FROM AUTHOR]

Background and Objectives: This study investigated different drying temperatures (40°C, 50°C, and 60°C) during the preparation of dehydrated quick‐cooking beans and verified the physical and chemical effects and digestibility of the grains. Two genotypes (BRS Esteio and BRS Estilo), freshly harvested and with hard‐to‐cook (HTC) defects, were used. Findings: Lower drying temperature favors producing quick‐cooking beans with reduced cooking times. As the temperature increases, there is an increase in grain browning. Higher temperature (60°C) promotes the most significant damage, reaching 36.35% in BRS Estilo. Phytic acid content reduced considerably at drying temperatures of 50°C and 60°C in HTC beans, and the lower content of tannin was obtained after drying at 40°C (0.45 and 0.60 mg/100 g). All quick‐cooking beans had over 90% protein digestibility. The development of quick‐cooking beans using a drying temperature of 40°C provided higher starch digestibility after 180 min of simulated intestinal digestion. Conclusions: The drying process at 40°C improved the technological, physical, and nutritional characteristics of quick‐cooking dehydrated beans. Significance and Novelty: The drying temperature directly influences the final quality of quick‐cooking beans; so, it is important to consider the use of low temperatures. [ABSTRACT FROM AUTHOR]

Coconut consumption provides nutritional and health benefits to humans. Yet, poor postharvest and preservation methods result in the loss of coconut fruit bunches yearly. Investigations into achieving drying techniques that result in optimum drying rates coupled with consumer's desired end products that are both commercially and nutritionally viable remain paramount in the food industry. Consequently, osmotic dehydration is commendable for its low-cost pretreatment merit. Therefore, the study examined the drying-kinetics, energy consumption, effective moisture diffusivity (Deff), vitamin C retention, color, and rehydration behavior of coconut cultivars (Sri Lanka Green Dwarf × Vanuatu Tall (SGD × VTT), Catigan (CAT), and Tacunan Green Dwarf (TGD)) meat slices. Samples were osmotically pretreated with sucrose solution (30 %/30 min) and subjected to oven drying (80, 90, 100, and 110 °C (air velocity of 2 m/s) and lyophilization (−45 ± 2 °C) methods. A mathematical model was employed to predict the effect of osmotic pretreatment on drying dynamics and the assessment of utilized energy, vitamin C content, color, and rehydration of coconut meat slices at different drying conditions. The oven-dried sample's color changed (p

Abstract [Objective] Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao is an important primitive plant of astragali radix, and its main planting areas are arid and semi-arid areas in Inner Mongolia, Shanxi, Gansu, and other places. Irregular intermittent rainfalls cause drought and rehydration cycle in plants. Studying the characteristics of metabolites in A . membranaceus var. mongholicus during drought and rehydration is crucial to understand the drought tolerance mechanism in response to natural intermittent rainfalls. [Methods] The seedlings of annual A . membranaceus var. mongholicus were used as materials. After repeated drought and rehydration treatment, soil nutrients and root growth in potted plants were measured. The primary metabolites of A . membranaceus var. mongholicus were analyzed using NMR based technology. Differential metabolite screening and metabolic pathway were analyzed. Total flavonoids, total saponins, and polysaccharides in the roots of A . membranaceus var. mongholicus were determined. [Results] (1) A . membranaceus var. mongholicus showed a trend of decreasing in root diameter and increasing in root length and fibrous root density under drought stress. (2) Under drought and rehydration treatment, a total of 42 metabolites were detected, containing mainly amino acids and their derivatives, organic acids, amines, ammonia compounds, and sugars. Metabolic pathway analysis showed that amino acid metabolism pathways were mainly affected during repeated drought stress. Plant drought resistance was enhanced by increasing the content of aspartate, alanine, glutamate, proline, and arginine, as well as reducing the content of asparagine, tryptophan, and 4-aminobutyrate. Specifically, the increase in metabolites such as alanine, glutamate, and proline was 1-3 times, while metabolites such as tryptophan and 4-aminobutyric acid were decreased by 1-2 times. (3) Under the first round of drought stress, the accumulation of astragaloside was decreased; after the second round of drought stress, the production of three active substances, namely astragaloside, total flavonoids, and polysaccharides, showed a downward trend. [Conclusion] Amino acids and their derivatives are closely related in response to drought stress in A . membranaceus var. mongholicus. Plant regulates its secondary metabolites in response to environmental changes

To investigate the effects of different drying methods on the rehydration and quality characteristic of low salt pickled abalone, this study used vacuum freeze drying, cold air drying and hot air drying to dry them. The mass volume and rehydration rate of the rehydration process were studied. Low field nuclear magnetic resonance (LF-NMR) and magnetic resonance imaging (MRI) were used to study the water distribution during the rehydration process and investigated the changes in the samples of color, texture, tissue morphology and free amino acids after rehydration. The results showed that the rehydration rate of vacuum freeze-dried abalone was 278.73% at 72 hours, which was significantly higher than that of cold-air dried abalone and hot-air dried abalone (P

[Objective] Crops often suffer from interval drought and rehydration (rainfall or irrigation) during the whole growth period, especially in arid and semi-arid areas. Under drought stress, the growth and development of crops are inhibited, and the yield is reduced; meanwhile, the plants drought tolerance will be induced. In order to compensate loss of yield or growth induced by drought stress, crop often shows compensatory after rehydration, which produces compensation or even over compensation effect on yield. The compensation effect of post drought is the result of crop’s positive response to drought stress and compensatory growth after rehydration. It can effectively improve crop yield and water use efficiency in crop production in arid areas. [Methods] Source sink relationship is widely used to explain the crop yield formation, and regulating the source sink relationship is one of key way to improve crop yield. The compensation effect of post drought is highly related to the source sink response of crops. Based on the current research progress, this paper tries to explain the compensation effect of post drought in source and sink way. [Results] Under the drought stress, in the source, the stomata were closed, photosynthesis was decreased and soluble carbohydrate accumulation was increased, while sink activity was often increased. After rehydration, the metabolism of soluble carbohydrate in the source (leaf) was enhanced, which enhanced the photosynthesis and the ability of carbohydrate synthesis; meanwhile, the sink activity (grain) was maintained at a high level, and the rate of assimilate unloading and accumulation was accelerated; the assimilate in the flow remained at a high level. During this process, the activities of sucrose invertase (INV), cell wall invertase (CW-INV) and fructan exohydrolases (1-FEH w3) play important roles in the source sink response to the compensation effect of post-drought. [Conclusion] This paper integrated the mechanism of compensation effect of post drought in the perspective of source sink relationship, which provides a theoretical basis for understanding the mechanism of compensation effect of post drought and improving crop water efficient in arid areas.

Noodles are popular staple foods globally, and dried noodle products (DNPs) have gained increasing attention due to recent changes in consumer diet behavior. Rapid rehydration and excellent texture quality are the two major demands consumers make of dried noodle products. Unfortunately, these two qualities conflict with each other: the rapid rehydration of DNPs generally requires a loose structure, which is disadvantageous for good texture qualities. This contradiction limits further development of the noodle industry, and overcoming this limitation remains challenging. Starch is the major component of noodles, and it has two main roles in DNPs. It serves as a skeleton for the noodle in gel networks form or acts as a noodle network filler in granule form. In this review, we comprehensively investigate the different roles of starch in DNPs, and propose strategies for balancing the conflicts between texture and rehydration qualities of DNPs by regulating the gel network and granule structure of starch. Current strategies in regulating the gel network mainly focused on the hydrogen bond strength, the orientation degree, and the porosity; while regulating granule structure was generally performed by adjusting the integrity and the gelatinization degree of starch. This review assists in the production of instant dried noodle products with desired qualities, and provides insights into promising enhancements in the quality of starch-based products by manipulating starch structure. [ABSTRACT FROM AUTHOR]

Purpose: Rapid gastric emptying and intestinal absorption of beverages is essential for rapid rehydration, and certain amino acids (AA) may augment fluid delivery. Three sugar-free beverages, containing differing AA concentrations (AA + PZ), were assessed for fluid absorption kinetics against commercial sugar-free (PZ, GZ) and carbohydrate-containing (GTQ) beverages. Methods: Healthy individuals (n = 15–17 per study) completed three randomised trials. Three beverages (550–600 mL) were ingested in each study (Study 1: AA + PZ [17.51 g/L AA], PZ, GZ; Study 2: AA + PZ [6.96 g/L AA], PZ, GZ; Study 3: AA + PZ [3.48 g/L AA], PZ, GTQ), containing 3.000 g deuterium oxide (D2O). Blood samples were collected pre-, 2-min, 5-min, and every 5-min until 60-min post-ingestion to quantify maximal D2O enrichment (Cmax), time Cmax occurred (Tmax) and area under the curve (AUC). Results: Study 1: AUC (AA + PZ: 15,184 ± 3532 δ‰ vs. VSMOW; PZ: 17,328 ± 3153 δ‰ vs. VSMOW; GZ: 17,749 ± 4204 δ‰ vs. VSMOW; P ≤ 0.006) and Tmax (P ≤ 0.005) were lower for AA + PZ vs. PZ/GZ. Study 2: D2O enrichment characteristics were not different amongst beverages (P ≥ 0.338). Study 3: Cmax (AA + PZ: 440 ± 94 δ‰ vs. VSMOW; PZ: 429 ± 83 δ‰ vs. VSMOW; GTQ: 398 ± 81 δ‰ vs. VSMOW) was greater (P = 0.046) for AA + PZ than GTQ, with no other differences (P ≥ 0.106). Conclusion: The addition of small amounts of AA (3.48 g/L) to a sugar-free beverage increased fluid delivery to the circulation compared to a carbohydrate-based beverage, but greater amounts (17.51 g/L) delayed delivery. [ABSTRACT FROM AUTHOR]

Copyright of Storage & Process is the property of Tianjin Academy of Agricultural Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Science & Technology of Food Industry is the property of Science & Technology of Food Industry Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

A direct flow evacuated tube solar dryer (DF_ETSD), a novel drying system, was used for drying pre-treated okra (Abelmoschus esculentus). The performance of DF_ETSD was analysed by determining thermal profiling, dryer and collector efficiency hourly. The maximum 3-day average ambient temperature, collector outlet temperature and solar radiation were 35.6 °C, 66.4 °C and 976 W m−2 respectively. The collector efficiency increased as solar radiation increased over time due to a higher temperature difference between the collector outlet and ambient temperature. The maximum collector and dryer efficiency observed were 30.19% and 21.47%, respectively. A pre-treatment of okra was done in hot water at 70, 80 and 90 °C for 5 min. Okra samples were dried from an initial moisture content of 87.42 ± 1.49% (wb) to a final value of 10.77 ± 1.03% (wb) in 9 h. The pre-treatment temperature of 80 °C is suitable for maximum drying rate, colour retention and rehydration ratio and minimum water activity, which signifies the longer shelf-life of okra. Midilli and Kucuk model was best fitted (highest R2, lowest χ2 and RMSE) for the control and samples pre-treated at 80 °C; however, Verma model was suitably fitted for the sample pre-treated at 70 and 90 °C. The payback period of DF_ETSD was found to be 1.27 years. Environmental analysis shows the CO2 emission and net CO2 mitigation ranged between 1.24 and 18.65 t and 9.86 and 154.05 t respectively for different selected lifecycles of the dryer. Due to its environmental sustainability and low payback period, the presented drying system is recommended for okra and other fruits and vegetables. [ABSTRACT FROM AUTHOR]

Spray drying is regarded as one of the most economical methods utilized in the production of liposome powder in industry. However, the poor reconstruction of the powder following rehydration imposes constraints on its potential utilization. In this study, three membrane stabilizers, Poloxamer 188 (P188), trehalose (Tre), and Tween 80 (T80) were selected due to their distinct localization behaviors, and their impact on the reconstruction of rehydrated liposomes was discussed. The mannitol-based spray-dried liposome powder with excellent reconstruction was successfully developed. After rehydrating the powder, the particle size (Dh) and polydispersion index (PDI) of mannitol-encapsulated liposome powder (Bla) significantly increase. Although adding leucine improved the dispersion of the powder (Bla/Leu), the increase in Dh and PDI after rehydration was more significant, changed from 172.79 nm and 0.075 for Bla to 459.97 nm and 0.291 for Bla/Leu, the micropolarity and hydrophobicity of membrane also increased, indicating a poorer reconstruction. The addition of extra membrane stabilizers enhanced the stability of the liposomes after rehydration. Specifically, the addition of T80 reduced the changes in Dh and PDI caused by leucine to 118.39 nm and 0.015, while the changes of ANS fluorescence intensity and I1/I3 to 178.66 a.u and 0.02. Raman spectroscopy revealed that the addition of membrane stabilizer greatly reduced the disorder of the acyl chain, especially the T80. It could be the embedding mode of T80 on liposomes and its excellent solubilization played the best role. It also showed the best stability in extreme environments such as acidity and ionic strength. [ABSTRACT FROM AUTHOR]

Background: Sour cherry has unique anthocyanin content, and it is rich in phenolic compounds. Dried sour cherry has a long shelf life and therefore may be a fine alternative to fresh fruit all year round. Aims: In this research, it was tried to evaluate the ability of ultrasonic method in reducing the drying time of sour cherry, while also introducing a mathematical model that best describes the drying kinetics. Methods: To apply ultrasound pre-treatment, the sour cherries were placed inside the ultrasonic bath device for 0, 4, 8, and 12 min, and after leaving the device and removing extra moisture, the samples in thin layers were placed in the dryer. In order to investigate the kinetics and predict the drying process of treated sour cherries, kinetic modeling was done with the help of experimental data and using the mathematical models. Results: The results showed that sonication treatment, causes an increase in moisture removal rate from the sour cherries, an increase in the effective moisture diffusivity coefficient, and as a result, reduces the drying time. By increasing the sonication time from 0 to 12 min, the average drying time of sour cherries in the hot-air dryer was decreased by approximately 27%. Also, increasing the sonication time from 0 to 12 min increased the average effective moisture diffusivity coefficient by 38%. The sonication time had no significant effect on the rehydration of dried sour cherries, and increasing pretreatment time from 4 to 12 min only increased the rehydration of the samples by 7%. The Page model was selected as the best model to predict moisture ratio changes under the selected experimental conditions. Conclusion: Generally, it is recommended to apply 12 min of ultrasound before the sour cherry drying process due to increasing the mass transfer rate and reducing the drying time. [ABSTRACT FROM AUTHOR]

In contrast to the freeze-drying process, the rehydration process of freeze-dried foods remains unclear. This study investigated the rehydration of freeze-dried soybean curd, also known as tofu, and elucidated its breakage mechanism during rehydration, which impairs texture. The rehydration of freeze-dried tofu was observed at different water temperatures (20, 40, 70, and 100 °C), with the required rehydration time found to decrease with increasing water temperature. Furthermore, tofu was found to absorb water faster in cracks formed during production than in the porous body. Crack expansion was observed only in hightemperature water, leading to breakage of the tofu. Environmental scanning electron microscopy revealed that tofu expanded when a sufficient amount of water was absorbed. Accordingly, crack expansion in hightemperature water is attributed to the stress concentration at the tip of the crack, which is caused by differences in the rehydration rate and resulting stiffness between the porous body and cracks. [ABSTRACT FROM AUTHOR]

Copyright of Acta Prataculturae Sinica is the property of Acta Prataculturae Sinica Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This present study reports the effect of different storage temperatures on the film‐dried chicken breast (FDCB) by film‐drying technique for 15 days, including FDCB‐(−18), FDCB‐4, FDCB‐18, FDCB‐28 and FDCB‐38 (°C). Both storage time and temperature negatively affected the water‐holding capacity and moisture ratio, except for FDCB‐(−18). To ensure the quality of film‐dried chicken meats, they were rehydrated and evaluated in terms of colour values, tissue microstructure and hardness. The results showed that the rehydrated samples FDCB‐28 and FDCB‐38 promoted redness loss and increased the hardness values compared with other samples at low temperatures. Moreover, the storage temperature below 28°C was the most effective strategy for best maintaining the physicochemical, antioxidative, microbiological and sensory quality of film‐dried chicken samples, similar to those of raw meat. Interestingly, the FDCB‐(−18), FDCB‐4 and FDCB‐18 samples showed a significant (p < 0.05) inhibitory effect on microbial growth and lipid oxidation for 15 days. [ABSTRACT FROM AUTHOR]

Purpose: In 2020, an outbreak of Salmonella Stanley linked to imported dried wood ear mushrooms affected 55 individuals in the United States of America. These mushrooms, commonly used in Asian cuisine, require processing, like rehydration and cutting, before serving. The US Centres for Disease Control and Prevention advise food preparers to use boiling water for rehydration to inactivate vegetative bacterial pathogens. Little is known about how food handlers prepare this ethnic ingredient and which handling procedures could enable Salmonella proliferation. Design/methodology/approach: This study used content analysis to investigate handling practices for dried wood ear mushrooms as demonstrated in YouTube recipe videos and to identify food safety implications during handling of the product. A total of 125 Chinese- and English-language YouTube videos were analysed. Findings: Major steps in handling procedures were identified, including rehydration, cutting/tearing and blanching. Around 62% of the videos failed to specify the water temperature for rehydration. Only three videos specified a water temperature of 100 °C for rehydrating the mushrooms, and 36% of the videos did not specify the soaking duration. Only one video showed handwashing, cleaning and sanitising of surfaces when handling the dried wood ear mushrooms. Practical implications: This study found that most YouTube videos provided vague and inconsistent descriptions of the rehydration procedure, including water temperature and soaking duration. Food preparers were advised to use boiling water for rehydration to inactivate vegetative bacterial pathogens. However, boiling water alone is insufficient to inactivate all bacterial spores. Extended periods of soaking and storage could be of concern for spore germination and bacterial growth. More validation studies need to be conducted to provide guidance on how to safely handle the mushrooms. Originality/value: This study will make a distinctive contribution to the field of food safety by being the first to investigate the handling procedure of a unique ethnic food ingredient, dried wood ear mushrooms, which has been linked to a previous outbreak and multiple recalls in the United States of America. The valuable data collected from this study can help target food handling education as well as influence future microbial validation study design and risk assessment. [ABSTRACT FROM AUTHOR]

In the Mediterranean region, reforestation programs record failures following successive drought periods. The use of different plant-growth-promoting amendments and the understanding of drought-induced physiological and biochemical responses of carob will contribute to the reforestation program's success. In this study, the effects of arbuscular-mycorrhizal-fungi (AMF), vermicompost (VC), and rock phosphate (RP) on carob seedlings under drought stress (DS) and recovery (REC) conditions were evaluated. A greenhouse experiment was conducted with carob seedlings grown in the presence of AMF, VC, and RP, applied alone or in combination under well-watered (WW), DS (by stopping irrigation for 12 days), and recovery (REC) conditions. The obtained results indicated that the triple combination (AMF + VC + RP) presented the highest improvement in water potential, photosynthetic pigment content, stomatal conductance, and chlorophyll fluorescence compared to the controls under DS and after REC. In addition, this combination resulted in improved tolerance of carob seedlings to DS and a high potential for rapid recovery after rehydration due to a high accumulation of sugars, proteins, and antioxidant enzymes. In summary, the results underline the importance of inoculating carob with AMF in combination with (in)-organic amendments in improving its tolerance to DS and its recovery performances. [ABSTRACT FROM AUTHOR]

Instant rice is well-suited for ready-to-use applications as low-moisture, light-weight military ration and emergency food for our Armed Forces, offering longer shelf life with rapid rehydration characteristics. Present investigation demonstrated the effect of different salt pretreatment during soaking as precooking operation on the physico-chemical, cooking and rehydration kinetics of instant rice. Application of salt pretreatment reduced bulk density and damaged grain percentage, while enhanced the porosity, volume expansion percent, weight gain percentage, and rehydration characteristics. The grain elongation ratio was not affected significantly by the application of salt pretreatments; however, water uptake and chemical composition were significantly affected. Soaking pretreatment with 1% calcium chloride, followed by open pan cooking and subsequently freeze-thaw-dehydrating until attainment of 5–6% moisture content was found to be the optimal processing condition for developing instant rice with less than 2 min of rehydration time by mere addition of hot water. Modelling of water absorption behaviour revealed that both Peleg (R2 0.980–0.999) and Singh and Kulshrestha (R2 0.966–0.999) models fitted well. [ABSTRACT FROM AUTHOR]

Sprouting enhances the nutritional and quality characteristics of mung beans (Vigna radiata L.). In this study, the impacts of infrared radiation and hot-air drying on microwave-pretreated mung bean sprouts were examined. The microwave process was performed for 0, 15, 30, 45, and 60 s before hot-air and infrared dehydration of sprouted mung beans. Microwave pretreatment decreased drying time of sprouted mung beans. Increasing the microwave pretreatment time from 0 to 60 s caused drying time of samples inside the hot-air dryer to decrease from 205 min to 130 min (p<0.05). Drying time of sprouts in the infrared dryer was significantly less than the hot-air dryer (p<0.05). Effective moisture diffusivity coefficient (Deff) calculated by Fick's second law, varied in the range of 1.06×10-10 - 1.60×10-10 m2 s -1, and 0.98×10-9 - 1.15×10-9 m2 s -1, for hot-air and infrared dried samples, respectively. Experimental data for drying curves were fitted to various thin-layer equations, and the Midilli equation was best suited to explain drying kinetics of sprouted mung beans. Average rehydration ratio of dried sprouted mung beans in hot-air and infrared dryers were 262.55%, and 211.65%, respectively. Briefly, the microwave pretreatment (about 60 sec) and use of infrared dryer is appropriate drying technique for sprouted mung beans with faster mass transfer and shorter drying time. [ABSTRACT FROM AUTHOR]

The effects of mixed culture fermentation with yeast, Lactobacillus bulgaricus and Streptococcus thermophilus on the quality of non-fried instant noodles were studied by rehydration test, texture analysis, electronic nose analysis and scanning electron microscopic (SEM) observation. The results showed that yeast largely affected the rehydration property and texture, while L. bulgaricus and S. thermophilus were mainly responsible for improving the flavor of non-fried instant noodles. The primary fermentation process was conducive to maintaining the cooking tolerance of non-fried instant noodles, while the secondary fermentation process was beneficial to improve the rehydration property and flavor, reducing the rehydration time to 4.0 min; the R(2) and R(7) responses of the rehydrated sample were 2.8 and 2.9, respectively. The SEM results showed that the samples had a uniform pore distribution after the secondary fermentation. Taken collectively, the optimal starter culture for the primary and secondary fermentation was 1.5% yeast + 0.075% L. bulgaricus + 0.075% S. thermophilus.

Abstract Drought stress can significantly affect plant growth, development, and yield. Fewer comparative studies have been conducted between different species of pines, particularly involving Pinus yunnanensis var. pygmaea (P. pygmaea). In this study, the physiological indices, photosynthetic pigment and related antioxidant enzyme changes in needles from P. pygmaea, P. elliottii and P. massoniana under drought at 0, 7, 14, 21, 28 and 35 d, as well as 7 days after rehydration, were measured. The PacBio single-molecule real-time (SMRT) and Illumina RNA sequencing were used to uncover the gene expression differences in P. pygmaea under drought and rehydration conditions. The results showed that the total antioxidant capacity (TAOC) of P. pygmaea was significantly higher than P. massoniana and P. elliottii. TAOC showed a continuous increase trend across all species. Soluble sugar (SS), starch content and non-structural carbohydrate (NSC) of all three pines displayed a "W" pattern, declining initially, increasing, and then decreasing again. P. pygmaea exhibits stronger drought tolerance and greater recovery ability under prolonged drought conditions. Through the PacBio SMRT-seq, a total of 50,979 high-quality transcripts were generated, and 6,521 SSR and 5,561 long non-coding RNAs (LncRNAs) were identified. A total of 2310, 1849, 5271, 5947, 7710, and 6854 differentially expressed genes (DEGs) were identified compared to the control (Pp0D) in six pair-wise comparisons of treatment versus control. bHLH, NAC, ERF, MYB_related, C3H transcription factors (TFs) play an important role in drought tolerance of P. pygmaea. KEGG enrichment analysis and Gene set enrichment analysis (GSEA) analysis showed that P. pygmaea may respond to drought by enhancing metabolic processes such as ABA signaling pathway, alpha-linolenic acid. Weighted gene co-expression network analysis (WGCNA) revealed GST, CAT, LEC14B, SEC23 were associated with antioxidant enzyme activity and TAOC. This study provides a basis for further research on drought tolerance differences among coniferous species.