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Chlormequat chloride (CCC), as a commonly used plant growth regulator in the production of rhizomatous medicinal herbs, can effectively control the bolting phenomenon in Angelica sinensis, significantly increasing the yield of underground rhizomes (medicinal part). However, its specific effects on the intrinsic quality of Angelica sinensis, especially medicinal components, require further investigation. The objective of this study is to conduct a thorough examination of CCC residue and its influence on the yield and medicinal components of Angelica sinensis. By spraying different concentrations of CCC on Angelica sinensis, we systematically monitored the final yield of Angelica sinensis Radix (ASR) in each treatment group and the residual concentration of CCC in ASR. Using UPLC-QTOF-MS technology, we conducted an in-depth analysis of the metabolic profile of ASR. Subsequently, UFLC-MS/MS was employed to accurately quantify the changes in the content of nine key active components in ASR. The results of this study indicate that the application of CCC significantly improves the yield of ASR, with the best effect observed at 0.1 g/L, resulting in a yield increase of 24.8%. Meanwhile, the residual amount of CCC in ASR is positively correlated with the application concentration, with the residual levels as high as 7.12 mg/kg in the high-concentration treatment group. Metabolomic analysis preliminarily identified 21 chemical components in ASR, including four organic acids and 13 phthalides. It is worth noting that the quantitative analysis results indicate significant changes in active components such as butylphthalide, Z-ligustilide, and ferulic acid after the application of CCC. Specifically, high-concentration CCC significantly increased the content of butylphthalide and levistolide A, while low-concentration CCC significantly promoted the accumulation of coniferyl ferulate and senkyunolide A, accompanied by a significant decrease in Z-ligustilide and ferulic acidy. In conclusion, while CCC use can increase yield, the associated increase in residues and imbalanced composition ratios may threaten the quality and safety of ASR. Therefore, it is crucial to control the amount of CCC used rationally to balance yield enhancement and quality assurance. [ABSTRACT FROM AUTHOR]

[Purpose] To analyze regulation through litigation in the Brazilian legal system, investigating its application in courts and its impact on the legal system and economic relations. [Methodology/approach/design] Theoretical approach based on literature review and analysis of relevant cases. [Findings] Regulation through litigation is an established practice in Brazil, although it is not extensively explored in the national doctrine. It is concluded that the Judiciary can also play a regulatory role, directly influencing the development of regulatory policies and the behavior of economic agents. [Originality/value] Regulation through litigation is a topic largely unexplored in the national doctrine, and this article aims to fill this gap by investigating and analyzing the application of this phenomenon in Brazilian courts.

Myofibrillar protein (MP) is an important protein in muscle, which can form a three-dimensional gel network structure upon heat treatment, ultimately influencing the quality of meat products. In the meat industry, hydrocolloids from different are incorporated to enhance the gel strength, water-holding capacity and functional properties of MP gel and finally optimize the quality of meat products. However, many factors affect the interaction between MP and hydrocolloids during the formation of heat-induced MP gelation, which in turn affects MP gel properties. In this article, the gelation mechanism of MP is systematically reviewed, the interaction mechanism between different hydrocolloids and MP is discussed, and the key factors influencing the regulatory effect of hydrocolloids on the formation of heat-induced MP gel and the effect of hydrocolloids on MP gel properties are summarized. We anticipate that this review will lay a theoretical foundation for establishing the key technical system based on hydrocolloids to regulate the quality of meat products and also provide technical guidance for future practical application.

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Chlormequat chloride (CCC), as a commonly used plant growth regulator in the production of rhizomatous medicinal herbs, can effectively control the bolting phenomenon in Angelica sinensis, significantly increasing the yield of underground rhizomes (medicinal part). However, its specific effects on the intrinsic quality of Angelica sinensis, especially medicinal components, require further investigation. The objective of this study is to conduct a thorough examination of CCC residue and its influence on the yield and medicinal components of Angelica sinensis. By spraying different concentrations of CCC on Angelica sinensis, we systematically monitored the final yield of Angelica sinensis Radix (ASR) in each treatment group and the residual concentration of CCC in ASR. Using UPLC-QTOF-MS technology, we conducted an in-depth analysis of the metabolic profile of ASR. Subsequently, UFLC-MS/MS was employed to accurately quantify the changes in the content of nine key active components in ASR. The results of this study indicate that the application of CCC significantly improves the yield of ASR, with the best effect observed at 0.1 g/L, resulting in a yield increase of 24.8%. Meanwhile, the residual amount of CCC in ASR is positively correlated with the application concentration, with the residual levels as high as 7.12 mg/kg in the high-concentration treatment group. Metabolomic analysis preliminarily identified 21 chemical components in ASR, including four organic acids and 13 phthalides. It is worth noting that the quantitative analysis results indicate significant changes in active components such as butylphthalide, Z-ligustilide, and ferulic acid after the application of CCC. Specifically, high-concentration CCC significantly increased the content of butylphthalide and levistolide A, while low-concentration CCC significantly promoted the accumulation of coniferyl ferulate and senkyunolide A, accompanied by a significant decrease in Z-ligustilide and ferulic acidy. In conclusion, while CCC use can increase yield, the associated increase in residues and imbalanced composition ratios may threaten the quality and safety of ASR. Therefore, it is crucial to control the amount of CCC used rationally to balance yield enhancement and quality assurance.

[Objective] An accelerated shift toward low carbon energy consumption is required in China due to environmental deterioration and energy security concerns. Clarifying the impact and mechanism of the Energy-consuming Right Trading System (ERTS) on the low-carbon transformation of the provincial energy consumption structure provides important policy implications for the development of market-based environmental regulation. [Methods] This study explored the effects of the ERTS from the perspective of a quasi-natural experiment, taking the four pilot provinces (Zhejiang, Fujian, Henan, and Sichuan) as the experimental group and the remaining 26 provinces (excluding Hong Kong, Macao, Taiwan, and Tibet due to missing data) as the control group. The year 2016, when the policy began to be implemented, was seen as the time of policy shocks. The difference-in-differences (DID) method was used to test the relationship between the ERTS and the low-carbon transformation of the energy consumption structure. [Results] (1) The ERTS significantly and positively promoted the low-carbon transformation of the energy consumption structure in the pilot provinces and neighboring provinces. (2) Green technological progress and reduced resource dependency were the main paths for the ERTS to promote the low-carbon transformation of the energy consumption structure, while the mechanisms of industrial structure upgrading and public environmental concerns were not yet available. (3) In central provinces, western provinces, and provinces with low environmental information disclosure levels, the ERTS made a greater contribution to the decarbonization of energy consumption structure. [Conclusion] This study confirmed that the ERTS can achieve a low-carbon transformation of regional energy consumption structure through green technological progress and reduced resource dependency. Therefore, it is recommended to actively and orderly expand the pilot scope of the ERTS. The heterogeneous effects of regional differences and the level of environmental information disclosure on the effectiveness of the ERTS should be fully understood to promote the green and high-quality development of regional economies.

Bambusa bambos (B.B) biomass is cellulose rich lignocellulosic material, containing 47.49% cellulose, 17.49% hemicellulose, 23.56% lignin was used as a potential substrate for bioethanol production. The research paper investigates the use of B.B biomass as a substrate for bio-ethanol production through a two-phase catalytic conversion process. Four water-regulated regimes were identified to optimize the conversion of lignocellulosic biomass to biofuel precursors. The catalytic hydrolysis of B.B using CuCl2 was conducted for 10 hours at 110˚C, in aprotic ionic liquid (1-Butyl-3-methylimidazolium chloride) medium. The concentrations of glucose and 5-hydroxymethylfurfural (5-HMF) were measured while varying the amount of water addition. Water played a crucial role in the conversion of cellulose to glucose and 5-HMF by influencing product yields through the interplay of transport properties like heat conduction and viscosity. The highest glucose yield was achieved at 60.82% when operating at a water inclusion rate of 115.72 µL water/h for a duration of 6 hours at 110˚C. On the other hand, the maximum HMF yield was observed as 5.84% at water inclusion rate of 77.15 µL water/h for 5 hours at 110˚C. Yeast mediated glucose fermentation resulted in a bioethanol concentration of 5.5 mg/mL utilizing 15 mg/mL of catalytically produced glucose at a temperature of 30°C. After catalytic hydrolysis, the ionic liquid was also efficiently recycled for a sustainable economy.

Presently, the utilization of chlormequat in Astragalus mongholicus Bunge (Leguminosae) cultivation is prevalent for augmenting rhizome (Astragali Radix) yield. However, indiscriminate and excessive chlormequat employment can detrimentally influence Astragali Radix quality and safety. This research aimed to comprehensively comprehend chlormequat risks and its influence on Astragali Radix metabolites. Diverse chlormequat concentrations were employed in Astragalus mongholicus cultivation, with subsequent analysis of residual chlormequat levels in Astragali Radix across treatment groups. Astragali Radix metabolic profiling was conducted through UPLC-QTOF-MS, and thirteen principal active components were quantified via UFLC-MS/MS. Findings revealed a direct correlation between chlormequat residue levels in Astragali Radix and application concentration, with high-dose residue surpassing 5.0 mg/kg. Metabolomics analysis identified twenty-six distinct saponin and flavonoid metabolites. Notably, the application of chlormequat led to the upregulation of seven saponins (e.g., astragaloside I and II) and downregulation of six flavonoids (e.g., methylnissolin-3-O-glucoside and astraisoflavan-7-O-β-d-glucoside). Quantitative analysis demonstrated variable contents of active ingredients due to differing chlormequat concentrations, leading to astragaloside I increase (14.59–62.55%) and isoastragaloside II increase (4.8–55.63%), while methylnissolin-3-O-glucoside decreased (22.18–41.69%), as did astraisoflavan-7-O-β-d-glucoside (21.09–47.78%). In conclusion, chlormequat application influenced multiple active components in Astragali Radix, causing constituent proportion variations. Elevated chlormequat concentrations led to increased active components alongside heightened chlormequat residues in Astragali Radix. Consequently, prudent chlormequat application during Astragali Radix production is imperative to avert potential detriments to its quality and safety. [ABSTRACT FROM AUTHOR]

Copyright of Asian Journals of Ecotoxicology is the property of Gai Kan Bian Wei Hui and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The rapid economic growth of China has caused significant adverse effects on the environment. Meanwhile, technological innovation, a fundamental driver of economic development and social progress, plays a pivotal role in mitigating haze pollution. This study comprehensively examines the influence of technological innovation on haze pollution in the Yangtze River urban agglomeration, using the STIRPAT model and analyzing research data from 2004 to 2020. Additionally, the study investigates the specific moderating role of urban spatial structure in the relationship between technological innovation and haze pollution. The findings reveal that technological innovation in the middle reaches of the Yangtze River urban agglomeration can effectively curtail haze pollution, and its impact extends to surrounding areas through spillover effect. The polycentric urban spatial structure significantly enhances the haze-reducing effect of technological innovation in the region. Notably, in cities with a strong level of environmental regulation, the urban spatial structure plays a substantial role in augmenting the haze-reducing impact of technological innovation. The policy implications of this research underscore the importance of continuous improvement in technological innovation within the middle reaches of the Yangtze River urban agglomeration. To address haze pollution in future development, the adoption of a polycentric development strategy and the establishment of sound environmental governance policies are recommended. [ABSTRACT FROM AUTHOR]

Biochar possesses water absorption properties and can impact soil permeability, acidity levels, and other relevant factors. However, its effects are inconsistent as it is highly susceptible to external climatic conditions. Therefore, it is crucial to ensure the stability of biochar during the treatment of saline alkali soil. Achieving this goal requires enhancing the regulation of saline alkali soil and improving the properties of the land, both of which are vital for maintaining optimal conditions for agricultural production. Moreover, research on the use of biochar has predominantly concentrated on acidic or low to medium salt soils with little attention given to soda saline alkali soil. The use of ameliorating agents on saline and alkaline land has been the focus of attention and research, and the application of biochar in most research soils for the acidic or low to medium salt land is a key component in strengthening the regulation of saline and alkaline land and the improvement of land properties to ensure agricultural production. In order to improve biochar, this study suggested applying it to a soda-saline area in Northeast China and adding polymeric aluminum iron sulphate (Pais) for biochar's joint regulation. The study method primarily involved adjusting the pseudo dissociation of various biochar and improvement additives to examine their impact on regulating soil physicochemical properties. The biochar addition amount categorized the groups into Bi0 (0%), Bi1 (1%), Bi2 (2%), and Bi3 (3%). Based on the inclusion of polymeric aluminum iron sulphate modifier, the soil was classified into four groups as Pais0 (0%), Pais1 (1%), Pais2 (2%), and Pais3 (3%). The groups (Bi0 and Pais0) without any added ingredients (0%) were set as controls. The results found that the biocharenhanced amendment greatly impacted the pH values of the saline-alkali soil. Notably, the soil's bulk density showed a remarkable decrease while its porosity showed a significant increase with the maximum bulk density difference of the groups being 0.43 g/cm³. Particularly, the improvement effect of the modified proportion (1% and 2%) was outstanding. Comparing various modified biochar ratios to the untreated group, the difference in soil moisture content was essentially greater than 25%, and the saturated water holding capacity of the soil reached its highest value of 45.89% in the modified ratios (2% and 3%). The sodium adsorption ratio of salinealkaline soil with the improved ratio (1% and 2%) was the smallest (0.1), but the enhanced soil enzyme activity and fungus suppression were both more prominently affected by the improved biochar. The method of preparing this amendment was relatively straightforward, thereby reducing the cost of land treatment significantly. The enhanced biochar exhibited excellent capabilities to alleviate the salt alkalinity of saline land and improve soil fertility. This feat was of great technical significance and should be considered a valuable reference for the agricultural sector. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Technology Economics is the property of Chinese Society of Technology Economics and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Green agriculture is anticipated to be the leading trend for achieving sustainable and high-quality development in the agricultural sector in the future. The success of credit guarantee policies in promoting green agricultural development is closely linked to the level of participation and response from farmers in securing agricultural credit guarantee loans. We examined how farmers in Xiji, Ningxia, perceive agricultural credit guarantee policies and their involvement in such loans by analyzing 706 survey responses. Our analysis utilized a combination of statistical techniques, including principal component analysis, Heckman's two-stage model, and moderating effect model. The results indicate that out of the 706 surveyed farmers, 29.32% of households (207 households) had knowledge of the agricultural credit guarantee policy. While 66.86% of households (472 households) expressed interest in participating in agricultural credit guarantee loans, only 23.65% of households actually took part or participated multiple times. The overall awareness of the agricultural credit guarantee policy among farmers and their participation rate are both low. An increase in farmers' awareness of the agricultural credit guarantee policy can have a significant impact on their willingness and frequency of participation. The farmer's understanding of the agricultural credit guarantee policy has a significant impact on their decision to participate in credit guarantee loans. However, this effect can vary based on the farmer's income level, household capital, and factors such as social security, personal characteristics, location, and type of household business. To improve the support provided to farmers, it is advised to increase their awareness and understanding of agricultural credit guarantee policies. Furthermore, loan products and services should be personalized according to the capital available to each farmer's household, and the agricultural credit guarantee system and process should be enhanced to provide better assistance. [ABSTRACT FROM AUTHOR]

The issuance of digital currency electronic payments (DC/EP), under the supervision of the People's Bank of China, will have a certain impact on commercial banks, and will further affect the areas of internet finance and traditional financing. This paper studies the regulatory performance of DC/EP in the post-lending market under the models of internet finance and bank financing, exploring their theoretical and practical significance. Through the construction of an enterprise profit function and the regulatory utility function under the models of internet finance and bank financing, this study explores the impact of using DC/EP on the post-lending market. The study proves the following:In the absence of government regulation, internet finance platforms will increase the leverage ratio of loans, in order to obtain excess profits. The emergence of regulatory authorities and the use of DC/EP will control the leverage ratio of internet finance platforms, purify market performance, and stabilize the market order. The application of DC/EP provides a risk control method for the financial market, and coupled with government regulatory measures, it will effectively regulate the existing market order of internet finance platforms and increase the credibility of DC/EP. Therefore, the management insights obtained from this study of the application of DC/EP and the government regulation of existing financing and lending methods in the financial sector have practical significance for the current market. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Soochow University Philosophy & Social Sciences Edition is the property of Soochow University and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Atmospheric vapor pressure deficit (VPD, indicative of atmospheric water conditions) has been identified as a major driver of global vegetation dynamics. Drylands, including deserts, temperate grasslands, savannas, and dry forests, are more sensitive to water conditions and affect carbon, nitrogen, and water cycles. However, our knowledge is limited on the way increasing VPD affects vegetation growth and evapotranspiration (ET) in global drylands. In this study, we used long-term satellite datasets combined with multiple statistical analyses to examine the relationship between the satellite-derived normalized difference vegetation index (NDVI), a proxy for vegetation growth, and ET to VPD across global drylands. We found that significant decreases in NDVI and ET predominantly influenced the NDVI (RVPD − NDVI) and ET (RVPD − ET) responses to VPD in both the savannas and dry forests of South American, African, and Australian savannas and dry forests, as well as in temperate grasslands (e.g., Eurasian steppes and American prairies). Notably, more than 60% of global drylands exhibited significantly negative RVPD − NDVI and RVPD − ET values. In contrast, the percentage of significantly negative RVPD − NDVI and RVPD − ET decreased to 60° N). In predominantly warm drylands (60° N~60° S), negative VPD effects were significantly and positively regulated by soil water availability, as determined by multiple linear regression models. However, these significant regulatory effects were not observed in cold drylands. Moving-window analyses further revealed that temporal changes in RVPD − NDVI and RVPD − ET were positively correlated with changes in the Standardized Precipitation Evapotranspiration Index (SPEI). In warm drylands, areas with increasing RVPD − NDVI and RVPD − ET over time showed an increasing trend in the SPEI, whereas areas with a decreasing SPEI showed a negative trend in RVPD − NDVI and RVPD − ET values over time. Given the increasing atmospheric dryness due to climate change, this study highlighted the importance of re-evaluating the representation of the role of water availability in driving the response of the carbon-water cycle to increased VPD across global drylands.

After the share structure reform, controlling shareholder embraces more intimate related-party transactions to tunnel. Under the split of the CEO duality, the agency of the controlling shareholder is more complicated, and its cost is higher. This article focuses on the impact of controlling shareholder features and board characteristics on related-party transactions and examines their influence under the regulation. The empirical research reveals: (1) Cash flow rights are partly negatively related to related-party transactions and deliver a U-shaped non-linear curve relationship, suggesting the "incentive-barrier" effect. Pyramid shareholdings provide a natural barrier for controlling shareholders to the tunnel. (2) From the second-largest shareholders to the tenth-largest shareholders, it is challenging to balance the hidden tunneling behavior of the controlling shareholder, and they are possible to conspire with the controlling shareholder. (3) The CEO duality can enhance the influence of controlling shareholder characteristics on related-party transactions. (4) Under the regulation of the board's independence, the influence of controlling shareholder characteristics has weakened on related-party transactions. These findings ascertain new proof on the influencing factors of related-party transactions. [ABSTRACT FROM AUTHOR]

Presently, the utilization of chlormequat in Astragalus mongholicus Bunge (Leguminosae) cultivation is prevalent for augmenting rhizome (Astragali Radix) yield. However, indiscriminate and excessive chlormequat employment can detrimentally influence Astragali Radix quality and safety. This research aimed to comprehensively comprehend chlormequat risks and its influence on Astragali Radix metabolites. Diverse chlormequat concentrations were employed in Astragalus mongholicus cultivation, with subsequent analysis of residual chlormequat levels in Astragali Radix across treatment groups. Astragali Radix metabolic profiling was conducted through UPLC-QTOF-MS, and thirteen principal active components were quantified via UFLC-MS/MS. Findings revealed a direct correlation between chlormequat residue levels in Astragali Radix and application concentration, with high-dose residue surpassing 5.0 mg/kg. Metabolomics analysis identified twenty-six distinct saponin and flavonoid metabolites. Notably, the application of chlormequat led to the upregulation of seven saponins (e.g., astragaloside I and II) and downregulation of six flavonoids (e.g., methylnissolin-3-O-glucoside and astraisoflavan-7-O-β-d-glucoside). Quantitative analysis demonstrated variable contents of active ingredients due to differing chlormequat concentrations, leading to astragaloside I increase (14.59–62.55%) and isoastragaloside II increase (4.8–55.63%), while methylnissolin-3-O-glucoside decreased (22.18–41.69%), as did astraisoflavan-7-O-β-d-glucoside (21.09–47.78%). In conclusion, chlormequat application influenced multiple active components in Astragali Radix, causing constituent proportion variations. Elevated chlormequat concentrations led to increased active components alongside heightened chlormequat residues in Astragali Radix. Consequently, prudent chlormequat application during Astragali Radix production is imperative to avert potential detriments to its quality and safety.

The rapid economic growth of China has caused significant adverse effects on the environment. Meanwhile, technological innovation, a fundamental driver of economic development and social progress, plays a pivotal role in mitigating haze pollution. This study comprehensively examines the influence of technological innovation on haze pollution in the Yangtze River urban agglomeration, using the STIRPAT model and analyzing research data from 2004 to 2020. Additionally, the study investigates the specific moderating role of urban spatial structure in the relationship between technological innovation and haze pollution. The findings reveal that technological innovation in the middle reaches of the Yangtze River urban agglomeration can effectively curtail haze pollution, and its impact extends to surrounding areas through spillover effect. The polycentric urban spatial structure significantly enhances the haze-reducing effect of technological innovation in the region. Notably, in cities with a strong level of environmental regulation, the urban spatial structure plays a substantial role in augmenting the haze-reducing impact of technological innovation. The policy implications of this research underscore the importance of continuous improvement in technological innovation within the middle reaches of the Yangtze River urban agglomeration. To address haze pollution in future development, the adoption of a polycentric development strategy and the establishment of sound environmental governance policies are recommended.

Natural polysaccharides (NPs) possess numerous health-promoting effects, such as liver protection, kidney protection, lung protection, neuroprotection, cardioprotection, gastrointestinal protection, anti-oxidation, anti-diabetic, and anti-aging. Nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant pathway is an important endogenous antioxidant pathway, which plays crucial roles in maintaining human health as its protection against oxidative stress. Accumulating evidence suggested that Nrf2 antioxidant pathway might be one of key regulatory targets for the health-promoting effects of NPs. However, the information concerning regulation of NPs on Nrf2 antioxidant pathway is scattered, and NPs show different regulatory behaviors in their different health-promoting processes. Therefore, in this article, structural features of NPs having regulation on Nrf2 antioxidant pathway are overviewed. Moreover, regulatory effects of NPs on this pathway for health-promoting effects are summarized. Furthermore, structure-activity relationship of NPs for health-promoting effects by regulating the pathway is preliminarily discussed. Otherwise, the prospects on future work for regulation of NPs on this pathway are proposed. This review is beneficial to well-understanding of underlying mechanisms for health-promoting effects of NPs from the view angle of Nrf2 antioxidant pathway, and provides a theoretical basis for the development and utilization of NPs in promoting human health.

The gut microbiota dysbiosis is a state which the physiological combinations of flora are transformed into pathological combinations caused by factors such as diets, pollution, and drugs. Increasing evidence shows that dysbiosis is closely related to many diseases. With the continuous development and utilization of marine resources, marine polysaccharides have been found to regulate dysbiosis in many studies. In this review, we introduce the types of dysbiosis and the degree of it caused by different factors. We highlight the regulating effects of marine polysaccharides on dysbiosis as a potential prebiotic. The mechanisms of marine polysaccharides to regulate dysbiosis including protection of intestinal barrier, regulatory effect on gut microbiota, alteration for related metabolites, and some other possible mechanisms were summarized. And we aim to provide some references for the high-value utilization of marine polysaccharides and new targets for the treatment of gut microbiota dysbiosis by this review.

Qi Li,1 Suna Cai,1 Mengjiao Li,1 Kab Ibrahim Salma,1 Xiaojie Zhou,1 Feiyu Han,2 Jinzhao Chen,2 Ting Huyan1,3 1Key Laboratory for Space Biosciences and Biotechnology, Institute of Special Environment Biophysics, School of Life Sciences, Northwestern Polytechnical University, Xi’an, 710072, People’s Republic of China; 2Shanxi Weiqidaguangming Pharmaceutical Co., Ltd, The First Medical Park, Economic & Technology Development Zone, Datong, 037000, People’s Republic of China; 3Institute of Flexible Electronics (IFE), Northwestern Polytechnical University, Xi’an, 710072, People’s Republic of ChinaCorrespondence: Ting HuyanKey Laboratory for Space Biosciences and Biotechnology, Institute of Special Environment Biophysics, School of Life Sciences, Northwestern Polytechnical University, Xi’an, 710072, People’s Republic of ChinaTel/Fax +86 29 8846 0332Email huyanting@nwpu.edu.cnAbstract: Nowadays, tumor has been the serious threat to human health and life. To further explore the mechanism of tumor genesis and development is necessarily for developing the effective treatment strategy. Extracellular vesicles are the vesicles secreted by almost all types of cells, and they play an important part in intercellular communication by transporting their cargoes. Immune cells are the vital components of the human defense system, which defense against infection and tumor through cytotoxicity, immune surveillance, and clearance. However, via release tumor-derived extracellular vesicles, tumor could induce immune cells dysfunction to facilitate its proliferation and metastasis. Studies have shown that tumor-derived extracellular vesicles play dual role on immune cells by their specific cargoes. Here, we reviewed the effects of tumor-derived extracellular vesicles on immune cells in recent years and also summarized their research progress in the tumor immunotherapy and diagnosis.Keywords: tumor-derived extracellular vesicles, immune cell, regulatory effects, drug delivery, immunotherapy

HOTAIRM1, a newly found long non-coding RNA (lncRNA), is abnormally expressed in diverse malignant tumors, regulated mRNA, miRNA, relevant target gene or protein through specific signal transduction pathways to function in mechanisms of proliferation, invasion and metastasis of tumor cells. HOTAIRM1 is closely related to the clinicopathology or molecular pathology and have shown great potential in therapy and predicting prognosis of patients.

Bambusa bambos (B.B) biomass is cellulose rich lignocellulosic material, containing 47.49% cellulose, 17.49% hemicellulose, 23.56% lignin was used as a potential substrate for bioethanol production. The research paper investigates the use of B.B biomass as a substrate for bio-ethanol production through a two-phase catalytic conversion process. Four water-regulated regimes were identified to optimize the conversion of lignocellulosic biomass to biofuel precursors. The catalytic hydrolysis of B.B using CuCl 2 was conducted for 10 hours at 110˚C, in aprotic ionic liquid (1-Butyl-3-methylimidazolium chloride) medium. The concentrations of glucose and 5-hydroxymethylfurfural (5-HMF) were measured while varying the amount of water addition. Water played a crucial role in the conversion of cellulose to glucose and 5-HMF by influencing product yields through the interplay of transport properties like heat conduction and viscosity. The highest glucose yield was achieved at 60.82% when operating at a water inclusion rate of 115.72 µL water/h for a duration of 6 hours at 110˚C. On the other hand, the maximum HMF yield was observed as 5.84% at water inclusion rate of 77.15 µL water/h for 5 hours at 110˚C. Yeast mediated glucose fermentation resulted in a bioethanol concentration of 5.5 mg/mL utilizing 15 mg/mL of catalytically produced glucose at a temperature of 30°C. After catalytic hydrolysis, the ionic liquid was also efficiently recycled for a sustainable economy., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2024 The Author(s).)

Summary: Non-syndromic cleft lip with or without cleft palate (nsCL/P) is a common congenital facial malformation with a multifactorial etiology. Genome-wide association studies (GWASs) have identified multiple genetic risk loci. However, functional interpretation of these loci is hampered by the underrepresentation in public resources of systematic functional maps representative of human embryonic facial development. To generate novel insights into the etiology of nsCL/P, we leveraged published GWAS data on nsCL/P as well as available chromatin modification and expression data on mid-facial development. Our analyses identified five novel risk loci, prioritized candidate target genes within associated regions, and highlighted distinct pathways. Furthermore, the results suggest the presence of distinct regulatory effects of nsCL/P risk variants throughout mid-facial development and shed light on its regulatory architecture. Our integrated data provide a platform to advance hypothesis-driven molecular investigations of nsCL/P and other human facial defects.

Abstract Ferritins play vital roles in maintenance of iron homeostasis as iron storage proteins. Recently, the immune function of ferritins have attracted increasing attention, especially their roles in defense against pathogenic infections. However, the immune regulatory mechanism of fish ferritins are not well known. In the present study, comparative digital gene expression (DGE) profiling was performed to explore the regulatory effects of the Megalobrama amblycephala ferritins (MamFers) using MamFers overexpressed and control L8824 cells (Ctenopharyngodon idella hepatic cell line). Clean reads were aligned to the C. idella genome and differential expression analysis was conducted with representative differentially expressed genes pointed out. On that basis, further studies were performed to verify two pivotal regulated pathways in L8824 and EPC (Epithelioma Papulosum Cyprini cell line) cells, respectively. The results showed that NLRC5 (NOD-like Receptor Family CARD Domain Containing 5) mediated the regulation of MamFers on expression of MHC I (Major Histocompatibility Complex Class I) and its chaperone β2M (Beta-2-Microglobulin) in L8824 cells. Then, β2M further mediated the regulation of MamFers on hepcidin expression, indicating that MamFers regulated the expression of hepcidin via NLRC5/MHC I/β2M axis. In addition, MamFers regulated the adhesion of Aeromonas hydrophila to EPC cells by regulating the expression of two extracellular matrix proteins Intgβ1 (integrin β1) and FN (fibronectin). In a word, the present study provided novel insights into the immune regulatory functions of fish ferritins. Highlights • Comparative DGE profiling was performed to explore the regulatory effects of MamFers. • NLRC5 mediated the regulation of MamFers on the expression of MHC I and β2M genes. • β2M could mediate the regulation of MamFers on hepcidin expression. • MamFers regulated bacterial adhesion by regulating the expression of Intgβ1 and FN. [ABSTRACT FROM AUTHOR]

Intestinal metabolites participate in various physiological processes, including energy metabolism, cell-to-cell communication, and host immunity. These metabolites mainly originate from gut microbiota and hosts. Although many host metabolites are dominant in intestines, such as free fatty acids, amino acids and vitamins, the metabolites derived from gut microbiota are also essential for intestinal homeostasis. In addition, some metabolites are only generated and released by gut microbiota, such as bacteriocins, short-chain fatty acids, and quorum-sensing autoinducers. In this review, we summarize recent studies regarding the crosstalk between pathogens and metabolites from different sources, including the influence on bacterial development and the activation/inhibition of immune responses of hosts. All of these functions would affect the colonization of and infection by pathogens. This review provides clear ideas and directions for further exploring the regulatory mechanisms and effects of metabolites on pathogens.

This work employs experiments and statistical modelling to characterize three water-regulated regimes (limited/sufficient/inhibited) in two-phase catalytic conversion of lignocelluloses to biofuel precursors. CuCl 2 -catalyzed conversion of two non-edible lignocelluloses (June grass, Sunn hemp) is performed for 24 h using a protic ionic liquid (Triethylammonium hydrogen sulfate) at 110 °C, wherein the temporal dynamics of glucose, 5-Hydroxymethylfurfural (5-HMF), Levulinic acid (LA), and Formic acid (FA) are measured by varying the water-addition. Water, being a substrate in cellulose hydrolysis to glucose (isomerized to fructose), and an inhibitor in fructose dehydration to 5-HMF, regulates product yields through interactions between transport (heat conduction and viscosity) and reaction (non-linear kinetics and activation energy). The yields of glucose and 5-HMF peak at 6 h to 60.2% and 10.2%, respectively, from Sunn hemp, and at 4 h to 62.7% and 12.8%, respectively, from June grass, while those of LA and FA peak at 8 h to 23% and 9.1%, respectively, from Sunn hemp, and at 5 h from 19.3% to 7.7%, respectively, from June grass. The protic ionic liquid is recovered (91.4%) through biphasic separation, glucose is separated (75%) using an anti-solvent, 5-HMF is separated (54%) by liquid-liquid extraction, and LA (58%) and FA (42%) are separated by reactive extraction. The regulatory effects of water on cellulose hydrolysis from Sunn hemp and June grass are quantified (R2 > 0.92) by a linear superposition of two Gaussian distributions representing 'ignited' and 'extinguished' reaction states that delineate the three water-regulated regimes, and allow us to quantify the water-sufficient regime for maximizing product yields. [Display omitted] • Biofuel precursors (glucose, HMF, levulinic and formic acids) from non-edible lignocelluloses (June grass, Sunn hemp). • Temporal dynamics of biofuel precursors from catalytic hydrolysis experiments. • Statistical modelling of regulatory effects of water addition on product yields. • Linear-superposition of two Gaussian distributions (extinguished and ignited states). • Product separation and ionic liquid recycling for cost-effectiveness. [ABSTRACT FROM AUTHOR]

Under the requirements of China's regional coordination and green low-carbon development strategy, using provincial balanced panel data from 1998 to 2017, this paper reveals how the spatial functional division affects carbon emissions and discusses the regulatory effect of transportation and communication infrastructure on the relationship between the spatial functional division and carbon emissions. The research conclusions are as follows. First, with the deepening of spatial functional division, the carbon emissions show a significant inverted U-shaped trend, and this result remains robust after taking into account location differences and spatial form. Second, the number of provinces where the spatial functional division exceeds the inflection point gradually increases; however, the level of spatial functional division in most provinces is insufficient. Third, the mechanism analysis indicates that market integration and technological progress are important channels through which spatial functional division affects carbon emissions. Finally, the enhancement of transportation and communication infrastructure contributes to the carbon reduction effect of the spatial functional division. In a context where the link between agglomeration and carbon emissions has been widely explored, this paper provides new evidence and cases for thinking about the synergistic development of industrial agglomeration and carbon emissions reduction from the perspective of functional division. [Display omitted] • The impact of spatial functional division on carbon emissions is explored. • The spatial functional division in several provinces beyond the inflection point. • Market integration and technological progress are important influencing paths. • Transportation and communication infrastructure have moderating effects. [ABSTRACT FROM AUTHOR]

Natural polysaccharides (NPs) possess numerous health-promoting effects, such as liver protection, kidney protection, lung protection, neuroprotection, cardioprotection, gastrointestinal protection, anti-oxidation, anti-diabetic, and anti-aging. Nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant pathway is an important endogenous antioxidant pathway, which plays crucial roles in maintaining human health as its protection against oxidative stress. Accumulating evidence suggested that Nrf2 antioxidant pathway might be one of key regulatory targets for the health-promoting effects of NPs. However, the information concerning regulation of NPs on Nrf2 antioxidant pathway is scattered, and NPs show different regulatory behaviors in their different health-promoting processes. Therefore, in this article, structural features of NPs having regulation on Nrf2 antioxidant pathway are overviewed. Moreover, regulatory effects of NPs on this pathway for health-promoting effects are summarized. Furthermore, structure-activity relationship of NPs for health-promoting effects by regulating the pathway is preliminarily discussed. Otherwise, the prospects on future work for regulation of NPs on this pathway are proposed. This review is beneficial to well-understanding of underlying mechanisms for health-promoting effects of NPs from the view angle of Nrf2 antioxidant pathway, and provides a theoretical basis for the development and utilization of NPs in promoting human health., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Luo, Li, Shen, Lin, Chen, Wang, Gong, Liu, Zou and Wang.)

The Ca2+-modulated, dimeric proteins of the EF-hand (helix-loop-helix) type, S100A1 and S100B, that have been shown to inhibit microtubule (MT) protein assembly and to promote MT disassembly, interact with the type III intermediate filament (IF) subunits, desmin and glial fibrillary acidic protein (GFAP), with a stoichiometry of 2 mol of IF subunit/mol of S100A1 or S100B dimer and an affinity of 0.5-1.0 µM in the presence of a few micromolar concentrations of Ca2+. Binding of S100A1 and S100B results in inhibition of desmin and GFAP assemblies into IFs and stimulation of the disassembly of preformed desmin and GFAP IFs. S100A1 and S100B interact with a stretch of residues in the N-terminal (head) domain of desmin and GFAP, thereby blocking the head-to-tail process of IF elongation. The C-terminal extension of S100A1 (and, likely, S100B) represents a critical part of the site that recognizes desmin and GFAP. S100B is localized to IFs within cells, suggesting that it might have a role in remodeling IFs upon elevation of cytosolic Ca2+ concentration by avoiding excess IF assembly and/or promoting IF disassembly in vivo. S100A1, that is not localized to IFs, might also play a role in the regulation of IF dynamics by binding to and sequestering unassembled IF subunits. Together, these observations suggest that S100A1 and S100B may be regarded as Ca2+-dependent regulators of the state of assembly of two important elements of the cytoskeleton, IFs and MTs, and, potentially, of MT- and IF-based activities.

Small firms are popularly viewed as resistant to complying with regulation. Harmonisation of Australia’s state-based work health and safety regimes is a significant regulatory change. In this article, we consider the likely responses of small firms to work health and safety harmonisation and argue that a range of choices are open to small firm owner-managers. These choices are shaped by individuals’ world views and are influenced by elements in the firms’ context. A significant element is the public narrative of work health and safety harmonisation, which can be understood by using discourse and sense-making concepts. Our analysis of small firm owner-manager choices takes into account small firms’ embeddedness in their regulatory context and the influence on organisational decision-making of the narrative of work health and safety harmonisation. The dominant narrative is arguably silent on the benefits of the work health and safety regulatory change and therefore the response of small firms is likely to be avoidance or minimalism. Non-compliance could be the result due to poor awareness of opportunities arising from this regulatory change. [ABSTRACT FROM PUBLISHER]

The gut microbiota dysbiosis is a state which the physiological combinations of flora are transformed into pathological combinations caused by factors such as diets, pollution, and drugs. Increasing evidence shows that dysbiosis is closely related to many diseases. With the continuous development and utilization of marine resources, marine polysaccharides have been found to regulate dysbiosis in many studies. In this review, we introduce the types of dysbiosis and the degree of it caused by different factors. We highlight the regulating effects of marine polysaccharides on dysbiosis as a potential prebiotic. The mechanisms of marine polysaccharides to regulate dysbiosis including protection of intestinal barrier, regulatory effect on gut microbiota, alteration for related metabolites, and some other possible mechanisms were summarized. And we aim to provide some references for the high-value utilization of marine polysaccharides and new targets for the treatment of gut microbiota dysbiosis by this review., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (© 2022 Published by Elsevier Ltd.)

The literature data on glycine metabolism in neural tissue, the mitochondrial Gly-cleaving system, and the Gly uptake system in neural and glial cells are summarized. Brief description of localization and abundance of glycine receptors and specific binding sites in the mammalian nervous tissue is given. Four types of glycine binding receptors are described. These include own specific glycine receptor (GlyR), ionotropic glutamate receptor, which selectively binds N-methyl-D-aspartate selectively (NMDAR), and ionotropic receptors of γ-aminobutyrate (GABAR, GABAR). [ABSTRACT FROM AUTHOR]

The biochemical formation of yeast-derived sensory-active metabolites like higher alcohols and esters determines the different characteristics of aroma and taste in fermented beverages. In yeast fermentation process, a large number of environmental factors affecting the production of volatile aroma compounds are abundant. Factors like substrate composition in fermentation media as well as process parameters influencing these flavor-active metabolites have already been described. These factors can act on the expression of yeast genes involved in aroma metabolism resulting in concentration differences in esters and higher alcohols important for flavor and taste. The understanding of the function of genes involved in biosynthetic pathways of aroma-active substances as well as their regulatory mechanisms is needed to control the production of ester and higher alcohol synthesis to create specific aroma profiles in fermented beverages. This review discusses the known regulation and function of several individual genes ( ATF1, ATF2, EEB1, EHT1, BAT1, BAT2 and BAP2) described in fusel alcohol and ester synthesis mainly in S. cerevisiae and S. pastorianus var. carlsbergensis. Also, different factors like oxygen and temperature that allow ester and higher alcohol synthesis to be controlled during yeast fermentation are described. [ABSTRACT FROM AUTHOR]

Cytosolic sulfotransferases (STs), traditionally viewed as Phase II drug-metabolizing or detoxifying enzymes, are increasingly being implicated in the metabolism of endogenous biologically-active molecules. Except for studies on changes in their levels of expression and activity in the early stage of development in mammals, very little is known about how these enzymes are regulated. In this study, the regulatory effects of divalent metal cations on the activity of human cytosolic STs were quantitatively evaluated. Results obtained indicate that all nine human cytosolic STs examined are partially or completely inhibited/stimulated by the ten divalent metal cations tested at 10mM concentration. Compared with the other metal cations, the inhibitory or stimulatory effect of Mg2+and Ca2+on the activities of the human cytosolic STs appeared to be relatively smaller. Concentration-dependent effects of the divalent metal cations were further examined. The IC50 or EC50 values determined for different divalent metal cations were mostly above their normal physiological concentration ranges. In a few cases, however, IC50 values close to the physiological concentrations of certain divalent metal cations were observed. Using the monoamine (M)-form phenol ST(PST) as a model, it was demonstrated that the Km for dopamine changed only slightly with increasing concentrations of Cd2+ whereas the Vmax was dramatically decreased. [ABSTRACT FROM AUTHOR]

Porcine skin nucleoplasmic extract (PSNE) was shown to alter the incorporation of [H]thymidine into DNA of selected porcine, bovine, and human cell populations in culture. PSNE stimulated incorporation of [H]thymidine into DNA of porcine and bovine dermal cells an average of 300 and 200% of control value, respectively. When porcine and bovine epidermal cells were exposed to PSNE the treatment inhibited [H]thymidine incorporation into DNA by an average of 48 and 45%, respectively. Similar inhibitions were observed for porcine and bovine kidney, porcine lung, and human KB cells. Thus, the effect of PSNE on the incorporation of [H]thymidine into DNA of various cultured cells was either stimulatory to dermal cells or inhibitory to a variety of other cell types, including skin epidermal cells. The stimulatory and inhibitory effects of PSNE were abolished by heating PSNE for 5 min in boiling water before its addition to cell cultures. This suggests that macromolecular structure is important in the action of PSNE. [ABSTRACT FROM AUTHOR]

The intervention of human in phosphorus pool seems to be a vicious circle. The rapid population growth leads to the global food shortage, which leads to the massive use of phosphate fertilizer and the continuous exploitation of phosphate rocks. With the massive loss and fixation of phosphate fertilizer in the soil, the unavailable phosphorus in the soil becomes superfluous, while the phosphate mineral resources turn to scarce. Interestingly, exogenous carbonaceous materials, notably, biochar and humic substances, have been widely used as soil conditioners in agricultural production up to date, among other actions to interfere with the balance between the different phosphate species, which offer effective roles for increasing soil available phosphorus. This article reviews the regulation mechanisms of biochar and humic substances on phosphorus availability and circulation, including improving soil physicochemical characteristics, regulating microbial community structure, and directly interacting with phosphorus to affect the fate of phosphorus in soil. Finally, the prospects for future research directions are made, and it is hoped that the review of this article can arouse people's attention to the current plight of agricultural production and provide some methods for improving the efficiency of phosphate fertilizer use in the future. Unlabelled Image • Mechanisms of biochar and humic substances affecting the behavior of soil phosphorus were systematically summarized. • Key interfacial behaviors of phosphorus with biochar and humic substances were revealed. • The novel hydrothermal humification technology to alleviate the phosphorus crisis was emphasized. [ABSTRACT FROM AUTHOR]

Non-syndromic cleft lip with or without cleft palate (nsCL/P) is a common congenital facial malformation with a multifactorial etiology. Genome-wide association studies (GWASs) have identified multiple genetic risk loci. However, functional interpretation of these loci is hampered by the underrepresentation in public resources of systematic functional maps representative of human embryonic facial development. To generate novel insights into the etiology of nsCL/P, we leveraged published GWAS data on nsCL/P as well as available chromatin modification and expression data on mid-facial development. Our analyses identified five novel risk loci, prioritized candidate target genes within associated regions, and highlighted distinct pathways. Furthermore, the results suggest the presence of distinct regulatory effects of nsCL/P risk variants throughout mid-facial development and shed light on its regulatory architecture. Our integrated data provide a platform to advance hypothesis-driven molecular investigations of nsCL/P and other human facial defects., Competing Interests: The authors declare no competing interests., (© 2021 The Authors.)