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Nanodiamonds (NDs) are utilized for various purposes in dentistry, such as tissue regeneration, gene therapy, and medication delivery. Despite the examination of their potential for dental uses, there is currently no published review summarizing the conducted investigations. Additional investigations are required to comprehend the biological compatibility of NDs in dentistry. NDs have been proven effective for a variety of applications in dentistry because of unique qualities such as target cell selectivity, nanometer size, and fluorescence. Nanodiamonds find various applications in dentistry, encompassing roles in directed tissue regeneration, reinforcing polymers, and administering medications for treating infections and cancer. Researchers have also suggested the potential use of nanodiamonds as coatings for antibacterial or bioactive dental implants. Nevertheless, investigations on their biological compatibility have been scarce and insufficient so far, necessitating significantly further studies to envision the potential use of NDs in dentistry. It is believed that within the ten years to come, these emerging substances will discover several kinds of daily usage in dental practice. [ABSTRACT FROM AUTHOR]

Background and Objectives: Cleft lip alone or a combination of cleft lip and palate (CLP) is a common developmental abnormality in the craniofacial region. This umbrella review aims to identify promising avenues for treatment using stem cell therapy. Materials and Methods: Systematic reviews from 2014 to 2024 were searched among databases like PubMed, Medline, and Google Scholar. PRISMA guidelines were employed to ensure the thoroughness of the search. A quality assessment (ROBIS) of the included reviews was conducted to ensure the reliability and validity of the synthesized evidence. Results: Five systematic reviews were selected for this umbrella review. Results show that stem cell therapy, specifically using mesenchymal stem cells (MSCs) and adipocyte stem cells (ADSCs), promotes bone regeneration in CLP deformities. Although multiple studies have established the effectiveness of diverse types of stem cells in treating CLP, important considerations including safety concerns, methodological variability, and the need for standardization have been identified. The fact that the number of relevant systematic reviews that matched our inclusion criteria was limited could affect this research's robustness and may limit the breadth and depth of evidence synthesis. Definitive conclusions could not be reached due to variation among treatments and outcomes. Conclusions: The examined studies highlight the potential of stem cell therapy as a complementary approach to existing treatments for CLP. However, there are challenges that need to be addressed, including concerns regarding safety, variations in methodologies, and the need for standardization. Exploring the potential of other stem cell types may further enhance treatment outcomes for CLP patients. [ABSTRACT FROM AUTHOR]

Aims: Dental pulp stem cells (DPSCs) contain a population of stem cells with a broad range of differentiation potentials, as well as more lineage‐committed progenitors. Such heterogeneity is a significant obstacle to experimental and clinical applications. The aim of this study is to isolate and characterize a homogenous neuronal progenitor cell population from human DPSCs. Methodology: Polysialylated‐neural cell adhesion molecule (PSA‐NCAM+) neural progenitors were isolated from the dental pulp of three independent donors using magnetic‐activated cell sorting (MACS) technology. Immunofluorescent staining with a panel of neural and non‐neural markers was used to characterize the magnetically isolated PSA‐NCAM+ fraction. PSA‐NCAM+ cells were then cultured in Neurobasal A supplemented with neurotrophic factors: dibutyryl cyclic‐AMP, neurotrophin‐3, B27 and N2 supplements to induce neuronal differentiation. Both PSA‐NCAM+ and differentiated PSA‐NCAM+ cells were used in Ca2+ imaging studies to assess the functionality of P2X3 receptors as well as membrane depolarization. Results: PSA‐NCAM+ neural progenitors were isolated from a heterogeneous population of hDPSCs using magnetic‐activated cell sorting and anti‐PSA‐NCAM MicroBeads. Flow cytometry analysis demonstrated that immunomagnetic sorting significantly increased the purity of PSA‐NCAM+ cells. Immunofluorescent staining revealed expression of pan‐neuronal and mature neuronal markers, PGP9.5 and MAP2, respectively, as well as weak expression of the mature sensory markers, peripherin and islet1. ATP‐induced response was mediated predominately by P2X3 receptors in both undifferentiated and differentiated cells, with a greater magnitude observed in the latter. In addition, membrane depolarizations were also detected in cells before and after differentiation when loaded with fast‐voltage‐responding fluorescent molecule, FluoVolt™ in response to potassium chloride. Interestingly, only differentiated PSA‐NCAM+ cells were capable of spontaneous membrane oscillations. Conclusions: In summary, DPSCs contain a population of neuronal progenitors with enhanced neural differentiation and functional neural‐like properties that can be effectively isolated with magnetic‐activated cell sorting (MACS). [ABSTRACT FROM AUTHOR]

This article contributes to the growing literature on human-soil relations by exploring how care for agricultural soils unfolds among farmers who engage with alternative agricultural practices across different productions and sites in Norway and Costa Rica. These farmers approach soil as a living being and seek to approach care with macro- and microorganisms in response to soil challenges and economic instabilities. The article follows recent literature on soil care in showing how agricultural practices challenge the dominant approach to soils as passive. However, the article argues the necessity of expanding on existing notions of care. This, I argue, involves ethnographically "unearthing" care: unpacking and situating a diversity of soil care practices, their human and other-than-human entanglements, and how these relations are conditioned by environmental, genealogical, sociocultural, temporal, epistemic, economic, and political mechanisms within and beyond the farm. Considering these variables is essential to keep soil care in the emerging literature from following a romanticizing path toward abstract individualism. [ABSTRACT FROM AUTHOR]

Transforming Wisconsin's dairy production from annual crop-based to perennial grassland-based is critical for human health and well-being, but the ramifications for land use under this scenario have not been explored. I considered whether the amount of milk currently produced (~14.4B kg yr−1) with the confinement model (~2.57 AU ha−1) could be produced with a grassland model (~1.6 AU ha−1) without displacing production of other food and feed crops. Converting all land currently producing grains and forages for dairy (~796k ha) to grazed grassland would result in ~57% less milk production because there would be fewer cows producing less milk per animal. While the current system efficiently feeds dairy cattle when considering only milk yield per animal, it does so at the expense of environmental and social well-being, which are fundamental to the agroecological equation. To match current milk production exclusively on grassland would require ~1.04 M additional hectares, displacing other land uses in ways that would be initially very disruptive with significant transition costs, but eventually would improve ecosystem, community, and individual well-being. Planning for and realizing this vision requires major investments in markets, policies, and technical support of place-making and enterprise transitions to make Wisconsin America's Dairy Grassland. [ABSTRACT FROM AUTHOR]

Aquaculture plays an important role in the world's productive, social, and economic growth. The production of low-trophic-level fish such as Clupeids, aimed at the sustainable growth of the aquaculture industry, is a desirable and promising strategy, considering that low-trophic-level species represent approximately 30% of global fish production and contribute directly to worldwide food security. Clupeids, consisting mainly of herring, sardines, shads, and anchovies, form one of the main fisheries resources in the oceans, and the use of fish meal and fish oil to manufacture feed used to nourish fish in aquaculture also affects aquatic productivity through the capture of species, including small pelagic fish. The effects of overfishing on low-trophic-level species can have major impacts on marine ecosystems, and reducing the pressure on these natural stocks would be of extreme ecological importance. Aquaculture is an important tool for alleviating hunger and poverty in developing countries, providing a source of high-quality animal protein and generating employment and income. In Brazil, the cultivation of the Brazilian sardine Sardinella brasiliensis has been shown to be a viable alternative in marine fish farming due to its biological characteristics and economic and ecological importance. The present review addressed the interrelationship between fishing and the cultivation of S. brasiliensis, and how marine fish farming can be used as a compensatory tool in the supply of small marine pelagic fish threatened by overfishing in different regions of the world. [ABSTRACT FROM AUTHOR]

The regenerative ABS is developed to avoid slip and to regenerate energy using two types of braking: friction and motor. This paper discusses the experimental results of combining the two types of braking which are applied to a quarter-electric vehicle model. The control algorithm is developed using sliding mode control (SMC), where continuous action is performed to produce motor braking torque while discrete action is performed to produce hydraulic braking torque (friction). Furthermore, the proposed control system also implements control coordination to distribute braking torque, enhances the occurrence of the non-slip process, and generates energy for vehicle batteries. From the experimental results, it is concluded that the slip ratio value can be used to obtain the optimum conditions for the braking process, where the hydraulic torque as the cause of the slip, is limited through the braking distribution algorithm. The proposed control system produces a response with a brake speed of 2 m/s and a 3.39% increase in battery SOC.

Over the course of the postglacial period has managed to add degrade a substantial portion of the world's potential agricultural land. The soil loss and degradation that has repeatedly impacted regional societies around the world resulted from agricultural practices that increased the physical loss of soil (erosion), reduced soil organic matter, changed pH (acidification) or salinity, and disrupted or altered communities of soil life. In the coming century, as continued soil degradation threatens global food security while the global population keeps rising it is imperative that farmers develop and adopt soilhealth building (regenerative) practices to solve a problem that has plagued societies throughout history. Growing evidence suggests that agricultural systems that combine cover crops, reduced tillage, and diverse crop rotations can reduce erosion, enhance soil health and rebuild soil organic matter to cultivate beneficial soil life and harvest both economic and environmental benefits. In the coming post-oil world, global food security would benefit from a global effort to promote soil restoration to help addresses the challenge of sustainably feeding the world, increase soil-based carbon sequestration, protect on-farm biodiversity and reduce off-farm water pollution. Because soil security sets a solid foundation for global food security, agricultural policies and subsidies should be reformed to encourage farmers to adopt regenerative, soil-building practices. [ABSTRACT FROM AUTHOR]

This paper considers the relationship between biophilia and well-being in a sample of users of urban green spaces in the city of Porto (Portugal) as a case study. The biophilia hypothesis is explored through the responses of 131 participants in a survey. Users of urban green spaces valued their provision of quiet and shade, with a high diversity of plant species and tranquillity. The survey also found that being satisfied with urban green spaces enhances self-perceived health status, which means that being satisfied with a green space that fulfils users' expectations can be a way to improve well-being in urban areas. Creating stimulating regenerative urban green spaces offers the possibility to experience significant connections to nature. [ABSTRACT FROM AUTHOR]

Introduction: nanoparticles are tiny-sized materials whose characteristics and properties mean that their association with dental materials is being investigated to ascertain their effects and possible benefits on tooth structures. This systematic review aimed to qualitatively collect in vitro studies that address the potential application of different nanoparticles in dental regeneration. Following an exhaustive search and article selection process, 16 in vitro studies that met our eligibility criteria were included. BG-NPs were analyzed across five studies, with three demonstrating their impact on the growth and differentiation of human hDPSCs. CS-NPs were examined in three studies, with findings from two indicating a significant effect on the differentiation of SCAPs. Nanoparticles' therapeutic potential and their stimulatory effect on promoting the regeneration of cells of the dentin-pulp complex have been proven. Their effect is altered according to the type of nanoparticle, concentration, and substances associated with them and, depending on these variables, they will affect the pulp, dentine, and dental cementum differently. [ABSTRACT FROM AUTHOR]

South American camelids (SACs) play an increasing role in veterinary care in Europe. Many alpacas or llamas presented to veterinarians suffer from anaemia, regularly with a packed cell volume (PCV) below 0.10 l/l, which is a life-threatening condition for the animals. This review article presents clinical and laboratory diagnostic tools for the diagnosis of anaemia in SACs. Clinical identification of anaemic animals can be performed by assessing the FAMACHA© score and the Body Condition Score (BCS), since anaemia in alpacas and llamas correlates with pale mucous membranes and a lowered BCS. Haematological examination of a blood sample can provide a more differentiated diagnosis of anaemia in SACs. A common finding is regenerative anaemia with an increased number of reticulocytes that is often caused by blood loss due to Haemonchus contortus. Changes in a blood smear from an alpaca or llama with regenerative anaemia may include normoblasts (nucleated red blood cells), anisocytosis, poikilocytosis, polychromasia, Howell-Jolly bodies or basophilic stippling. Furthermore, non-regenerative anaemia, often caused by trace element deficiency or cachexia, can also occur. [ABSTRACT FROM AUTHOR]

Urine-derived stem cells (USCs) have gained the attention of researchers in the biomedical field in the past few years . Regarding the several varieties of cells that have been used for this purpose, USCs have demonstrated mesenchymal stem cell-like properties, such as differentiation and immunomodulation. Furthermore, they could be differentiated into several lineages. This is very interesting for regenerative techniques based on cell therapy. This review will embark on describing their separation, and profiling. We will specifically describe the USCs characteristics, in addition to their differentiation potential. Then, we will introduce and explore the primary uses of USCs. These involve thier utilization as a platform to produce stem cells, however, we shall concentrate on the utilization of USCs for therapeutic, and regenerative orofacial applications, providing an in-depth evaluation of this purpose. The final portion will address the limitations and challenges of their implementation in regenerative dentistry.

Morgan P Lorio,1 Jordan Lee Tate,2 Thomas J Myers,3 Jon E Block,4 Douglas P Beall5 1Advanced Orthopedics, Altamonte Springs, FL, USA; 2Southern Pain and Spine Associates, Jasper, GA, USA; 3Paradigm Health System, Slidell, LA, USA; 4Private Practice, San Francisco, CA, USA; 5Comprehensive Specialty Care, Edmond, OK, USACorrespondence: Jon E Block, Private Practice, 2210 Jackson Street, Ste. 401, San Francisco, CA, 94115, USA, Tel +1(415) 775-7947, Email jb@drjonblock.comAbstract: Specific clinical diagnostic criteria have established a consensus for defining patients with lumbar discogenic pain. However, if conservative medical management fails, these patients have few treatment options short of surgery involving discectomy often coupled with fusion or arthroplasty. There is a rapidly-emerging research effort to fill this treatment gap with intradiscal therapies that can be delivered minimally-invasively via fluoroscopically guided injection without altering the normal anatomy of the affected vertebral motion segment. Viable candidate products to date have included mesenchymal stromal cells, platelet-rich plasma, nucleus pulposus structural allograft, and other cell-based compositions. The objective of these products is to repair, supplement, and restore the damaged intervertebral disc as well as retard further degeneration. In doing so, the intervention is meant to eliminate the source of discogenic pain and avoid surgery. Methodologically rigorous studies are rare, however, and based on the best clinical evidence, the safety as well as the magnitude and duration of clinical efficacy remain difficult to estimate. Further, we summarize the US Food and Drug Administration’s (FDA) guidance regarding the interpretation of the minimal manipulation and homologous use criteria, which is central to designating these products as a tissue or as a drug/device/biologic. We also provide perspectives on the core evidence and knowledge gaps associated with intradiscal therapies, propose imperatives for evaluating effectiveness of these treatments and highlight several new technologies on the horizon.Keywords: degenerative, disc, intradiscal, autologous, allogeneic, injection, regenerative, discogenic, pain

The regenerative ABS is developed to avoid slip and to regenerate energy using two types of braking: friction and motor. This paper discusses the experimental results of combining the two types of braking which are applied to a quarter-electric vehicle model. The control algorithm is developed using sliding mode control (SMC), where continuous action is performed to produce motor braking torque while discrete action is performed to produce hydraulic braking torque (friction). Furthermore, the proposed control system also implements control coordination to distribute braking torque, enhances the occurrence of the non-slip process, and generates energy for vehicle batteries. From the experimental results, it is concluded that the slip ratio value can be used to obtain the optimum conditions for the braking process, where the hydraulic torque as the cause of the slip, is limited through the braking distribution algorithm. The proposed control system produces a response with a brake speed of 2 m/s and a 3.39% increase in battery SOC. [ABSTRACT FROM AUTHOR]

In the face of multiple crises, there is a pressing need to increase knowledge, skills and commitment to sustainability and climate action both amongst tourism practitioners and academics. There is an opportunity to deepen the concept of literacy in ways that not only capture operational aspects of tourism and carbon, but also embrace cultural and contextual knowledge and practices, so that the host-guest relationship is mutually enriching and contributes to regenerating destinations. This conceptual paper proposes a pathway that moves beyond building domain-specific carbon literacy to growing "Green Service Literacy" and ultimately "Regenerative Literacy." The vision of Regenerative Literacy connects to deep ecology thinking and decade-long efforts such as those proffered in the Earth Charter. Ultimately, it seeks to (re-)align our lifestyles with nature and ensure the human footprint remains within planetary boundaries. Changes across the tourism system are recommended to enable this transformation. [ABSTRACT FROM AUTHOR]

Introduction: Initial macrophage response to biostimulatory substances is key in determining the subsequent behavior of fibroblasts and the organization of newly synthesized collagen. Though histological studies suggest that calcium hydroxylapatite (CaHA) filler initiates a regenerative healing response with collagen and elastin deposition similar to natural, healthy tissue rather than an inflammatory response with fibrosis, the relative activity of macrophages stimulated by CaHA, as well as how this activity compares to that induced by other biostimulatory fillers, has not been explored. The aim of the study is to characterize the in vitro macrophage response to two biostimulory fillers, CaHA and PLLA (poly‐L lactic acid), and to evaluate their inflammatory potential. Methods: Primary human macrophages were incubated with two dilutions (1:50 and 1:100) of commercially available CaHA or PLLA. After 24 h incubation, an inflammation array was used to screen for the expression of 40 cytokines, released by macrophages. ELISA was used to confirm array results. Results: Four cytokines were significantly upregulated in M1 macrophages incubated with PLLA compared to both unstimulated controls and CaHA: CCL1 (p < 0.001), TNFRII (p < 0.01), MIP‐1α (p < 0.05), and IL‐8 (p < 0.001). In M2 macrophages, MIP‐1α (p < 0.01) and MIP‐1β (p < 0.01) were significantly upregulated by PLLA compared to CaHA and unstimulated controls. Conclusion: Together, these findings indicate that the CaHA mode of action is a non‐inflammatory response while PLLA initiates expression of several cytokines known to play a role in inflammation. Our study supports the concept that these two "biostimulatory" fillers follow distinct pathways and should be considered individually with regard to mechanism of action. [ABSTRACT FROM AUTHOR]

The Latin roots of the word reparations are "re" (again) plus "parere" which means "to give birth to, bring into being, produce". Together they mean "to make generative once again". In this sense, the extraction processes that cause labor injustice, ecological devastation, and social degradation cannot be repaired by simply transferring money. Reparations need to take on the full sense of "restorative": the transition to a decolonial system that can support value generators in the control of their own systems of production, protect the value they create from extraction, and circulate value in unalienated forms that benefit the human and non-human communities that produced that value. With funding from the National Science Foundation, we have developed a research framework for this process that starts with "artisanal labor": employee-owned business and worker collectives that have people doing what they love, despite low incomes. Focusing primarily on Detroit's Black-owned urban farms, artisanal textile businesses, Black hair salons, worker collectives, and other community-based production, with additional connections to Indigenous and other communities, we have introduced digital fabrication technologies, sensors, artificial intelligence, server-side apps and other computational support for a transition to unalienated circular value flow. We will report on our investigations with the challenges at multiple scales. At each level, we show how computational supports can act as restorative mechanisms for lost circular value flows, and thus address both past and ongoing disenfranchisement. [ABSTRACT FROM AUTHOR]

Copyright of Neutrosophic Computing & Machine Learning is the property of Multimedia Larga and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Introduction: Baseline expression of p16INK4a characterises cellular senescence. Similarly, the measurement of γ-GammaH2AX (γ-H2AX) foci levels in cells provides a reliable method for quantification of Deoxyribonucleic Acid (DNA) damage. Aim: To explore the role of p16INK4a and γ-H2AX as biomarkers of senescence in skin tissue. Materials and Methods: This was a cross-sectional study conducted in the Department of Anatomy, PGIMER, Chandigarh, Punjab, India from June 2022 to January 2023. Skin tissue was obtained from 30 cadavers, aged 20-90 years, from the anterior abdominal wall. The time duration of sample collection after death varied from six hours to 12 hours. Samples were divided into two groups: Group-I 70 years, with n=15 in each group. The relative change in the expression pattern of p16INK4a and γH2AX markers, as well as the microstructure of the skin (thickness of epidermis and dermis, distribution of collagen I/II/III fibres, architecture of sebaceous glands), were statistically analysed using an unpaired t-test. Results: Intense staining was observed with p16INK4a in Group-II, showing positivity in 60.75% of the cells, while Group-I depicted a weak staining pattern (15%). On immunostaining with γ-H2AX, only Group-II cells showed intense positivity (45%). Significant differences were observed in the epidermal and dermal thickness: Group-I (165.5267±37.73 μm; 2394.6±874.13 μm); Group-II (54.6±22.79 μm; 566.67±242.23 μm), and collagen Type-II/III fibres were found predominantly in aging skin tissue. Conclusion: The present study provides comprehensive data regarding age-associated changes between p16INK4a, γ-H2AX, and skin microstructure, which result in decreased repair and regenerative capacity of skin tissue and various age-related skin diseases.

Copyright of Riwayat: Educational Journal of History & Humanities is the property of Riwayat: Educational Journal of History & Humanities and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of TDRI Quarterly Review is the property of Thailand Development Research Institute and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

With the rapid development of stem cell research in modern times, stem cell-based therapy has opened a new era of tissue regeneration, becoming one of the most promising strategies for currently untreatable retinal diseases. Among the various sources of stem cells, adipose tissue-derived mesenchymal stem cells (ADSCs) have emerged as a promising therapeutic modality due to their characteristics and multiple functions, which include immunoregulation, anti-apoptosis of neurons, cytokine and growth factor secretion, and antioxidative activities. Studies have shown that ADSCs can facilitate the replacement of dying cells, promote tissue remodeling and regeneration, and support the survival and growth of retinal cells. Recent studies in this field have provided numerous experiments using different preclinical models. The aim of our review is to provide an overview of the therapeutic strategies, modern-day clinical trials, experimental models, and potential clinical use of this fascinating class of cells in addressing retinal disorders and diseases. [ABSTRACT FROM AUTHOR]

The expression of receptor activator of Nuclear Factor Kappa Beta (RANK) and its ligand (RANKL), as well as osteoprotegrin (OPG), in the alveolar bone (AB), may improve bone remodeling during orthodontic tooth movement (OTM). It is hypothesized that hypoxia-preconditioned gingival mesenchymal stem cells (GMSC) may be more effective than normoxia-preconditioned GMSC in this regard. This study aims to investigate the expression of RANK, RANKL, and OPG in the compression and tension sides of AB after allogeneic administration of GMSC that were normoxia or hypoxia-preconditioned in rabbits (Oryctolagus cuniculus) undergoing OTM. Twenty-four healthy young male rabbits were divided into two groups: T1, which underwent OTM and received normoxia-preconditioned GMSC, and T2, which underwent OTM and received hypoxia-preconditioned GMSC. A ligature wire was attached to the mandibular first molar and connected to a 50 g/mm2 closed coil spring, exerting force on the central incisor and left mandibular molar of the experimental animals. After 24 h of OTM, either normoxia- or hypoxia-preconditioned GMSC were injected into the gingiva of the samples in a single dose of 20 μl of phosphate-buffered saline (PBS). All samples were sacrificed on days 7, 14, and 28, and immunohistochemistry was performed to analyze the expression of RANK, RANKL, and OPG on the tension and compression sides. The expressions of RANK-RANKL-OPG in the alveolar bone of the compression and tension sides were significantly different during the 14-day period of OTM following allogeneic administration of GMSC that were normoxia or hypoxia-preconditioned (p < 0.05). The expression of RANK-RANKL was significantly increased on the compression side of the alveolar bone during OTM after the administration of hypoxia-preconditioned allogeneic GMSC but not on the tension side. Conversely, RANKL-OPG expression was enhanced on the tension side but not on the compression side, as observed through immunohistochemical analysis in vivo. [Display omitted] • Allogenic GMSCs Hypoxia Application during OTM in vivo enhance RANKL-OPG expression in alveolar bone of compression and tension side than Allogenic GMSCs Normoxia Application in day 14 immunohistochemically in vivo. • In the compression side of alveolar bone during OTM, RANK-RANKL expression was significantly enhanced after the application of GMSCs hypoxic-preconditioned allogeneic but not in tension side immunohistochemically in vivo. • RANKL-OPG expression significantly improved in the tension side but not in compression side immunohistochemically in vivo. [ABSTRACT FROM AUTHOR]

Introduction: Baseline expression of p16INK4a characterises cellular senescence. Similarly, the measurement of 1-Gamma-H2AX (1-H2AX) foci levels in cells provides a reliable method for quantification of Deoxyribonucleic Acid (DNA) damage. Aim: To explore the role of p16INK4a and 1-H2AX as biomarkers of senescence in skin tissue. Materials and Methods: This was a cross-sectional study conducted in the Department of Anatomy, PGIMER, Chandigarh, Punjab, India from June 2022 to January 2023. Skin tissue was obtained from 30 cadavers, aged 20-90 years, from the anterior abdominal wall. The time duration of sample collection after death varied from six hours to 12 hours. Samples were divided into two groups: Group-I <30 years and Group-II >70 years, with n=15 in each group. The relative change in the expression pattern of p16INK4a and 1H2AX markers, as well as the microstructure of the skin (thickness of epidermis and dermis, distribution of collagen I/II/III fibres, architecture of sebaceous glands), were statistically analysed using an unpaired t-test. Results: Intense staining was observed with p16INK4a in Group-II, showing positivity in 60.75% of the cells, while Group-I depicted a weak staining pattern (15%). On immunostaining with 1-H2AX, only Group-II cells showed intense positivity (45%). Significant differences were observed in the epidermal and dermal thickness: Group-I (165.5267±37.73 µm; 2394.6±874.13 µm); Group-II (54.6±22.79 µm; 566.67±242.23 µm), and collagen Type-II/III fibres were found predominantly in aging skin tissue. Conclusion: The present study provides comprehensive data regarding age-associated changes between p16INK4a, 1-H2AX, and skin microstructure, which result in decreased repair and regenerative capacity of skin tissue and various age-related skin diseases. [ABSTRACT FROM AUTHOR]

The flexible broadband communication system (FBCS) of the GEO-KOMSAT-3 satellite consists of a digital and analog hybrid type, and the digital signal is processed using digital processing equipment (DPE). Digital signal processing device design includes transparent and regenerative signal relay functions and interfaces with payload, TC/TM, and operation plan design for operation in the ground Spacecraft Operation Center (SOC) and Payload Operation Center (POC). In this study, the development status of the DPE mounted on GEO-KOMSAT-3 is discussed, and the main functions to be considered when designing a geostationary DPE based on the actual design for each function are explained. [ABSTRACT FROM AUTHOR]

Collagen VI and collagen XII are structurally complex collagens of the extracellular matrix (ECM). Like all collagens, type VI and XII both possess triple-helical components that facilitate participation in the ECM network, but collagen VI and XII are distinct from the more abundant fibrillar collagens in that they also possess arrays of structurally globular modules with the capacity to propagate signaling to attached cells. Cell attachment to collagen VI and XII is known to regulate protective, proliferative or developmental processes through a variety of mechanisms, but a growing body of genetic and biochemical evidence suggests that at least some of these phenomena may be potentiated through mechanisms that require coordinated interaction between the two collagens. For example, genetic studies in humans have identified forms of myopathic Ehlers-Danlos syndrome with overlapping phenotypes that result from mutations in either collagen VI or XII, and biochemical and cell-based studies have identified accessory molecules that could form bridging interactions between the two collagens. However, the demonstration of a direct or ternary structural interaction between collagen VI or XII has not yet been reported. This Hypothesis and Theory review article examines the evidence that supports the existence of a functional complex between type VI and XII collagen in the ECM and discusses potential biological implications.

With the rapid development of synthetic biology, recombinant human collagen has emerged as a cutting-edge biological material globally. Its innovative applications in the fields of material science and medicine have opened new horizons in biomedical research. Recombinant human collagen stands out as a highly promising biomaterial, playing a pivotal role in crucial areas such as wound healing, stroma regeneration, and orthopedics. However, realizing its full potential by efficiently delivering it for optimal therapeutic outcomes remains a formidable challenge. This review provides a comprehensive overview of the applications of recombinant human collagen in biomedical systems, focusing on resolving this crucial issue. Additionally, it encompasses the exploration of 3D printing technologies incorporating recombinant collagen to address some urgent clinical challenges in regenerative repair in the future. The primary aim of this review also is to spotlight the advancements in the realm of biomaterials utilizing recombinant collagen, with the intention of fostering additional innovation and making significant contributions to the enhancement of regenerative biomaterials, therapeutic methodologies, and overall patient outcomes.

Introduction: nanoparticles are tiny-sized materials whose characteristics and properties mean that their association with dental materials is being investigated to ascertain their effects and possible benefits on tooth structures. This systematic review aimed to qualitatively collect in vitro studies that address the potential application of different nanoparticles in dental regeneration. Following an exhaustive search and article selection process, 16 in vitro studies that met our eligibility criteria were included. BG-NPs were analyzed across five studies, with three demonstrating their impact on the growth and differentiation of human hDPSCs. CS-NPs were examined in three studies, with findings from two indicating a significant effect on the differentiation of SCAPs. Nanoparticles’ therapeutic potential and their stimulatory effect on promoting the regeneration of cells of the dentin-pulp complex have been proven. Their effect is altered according to the type of nanoparticle, concentration, and substances associated with them and, depending on these variables, they will affect the pulp, dentine, and dental cementum differently.

Cell therapies commonly pursue tissue stimulation for regenerative purposes by replacing cell numbers or supplying for functional deficiencies. To this aim, monodispersed cells are usually transplanted for incorporation by local injection. The limitations of this strategy include poor success associated with cell death, insufficient retention, or cell damage due to shear forces associated with the injection. Spheroids have recently emerged as a model that mimics an in vivo environment with more representative cell-to-cell interactions and better intercellular communication. Nevertheless, cost-effective and lab friendly fabrication and effectively performed recovery are challenges that restrict the broad application of spheroids. In this work, glass surfaces were modified with an environmentally friendly superhydrophobic coating. The superhydrophobic surfaces were used for the 3D spheroid preparation of fibroblasts (3T3 cell line) and keratinocytes (HaCaT cell line). The effectiveness of the spheroids to be recovered and grown under 2D culture conditions was evaluated. The morphology of the migrated cells from the 3D spheroids was characterized at the nano-microscale through 3D profilometry. The results demonstrated improved adhesion and proliferation in the migrated cells, both advanced properties for regenerative applications. [ABSTRACT FROM AUTHOR]

Over the years, aquaponics has become a powerful technological tool that allows the sustainable production of food, integrating conventional fish farming with vegetable production. The present study evaluated the production of late seedlings of açai Euterpe oleraceae in an aquaponic system with tambaqui Colossoma macropomum. A total of 36 tambaquis with an initial average weight and length of 1086.75 ± 16.38 g and 38.49 ± 0.90 cm were distributed in 12 independent aquaponic units, totaling 3.62 kg m−3. The fish were fed three times daily with commercial feed at a rate of 3%. Three flooding levels of 5, 10 and 15 cm, with constant water flow through the hydroponic bed (0.5 m2), were evaluated, and a control-hydroponic bed with flooding levels of 10 cm was established, all in triplicate. In the 5 and 10 cm treatments, 3450 açai seedlings with an initial height of 12.3 ± 1.9 cm were used (575 per aquaponic units), while the 15 cm treatment contained non-germinated açai seeds. The control group did not receive açai seeds and remained empty. Analyses to monitor total dissolved solids (TDS), electrical conductivity, dissolved oxygen, temperature, pH, alkalinity, hardness, nitrogenous compounds, and phosphate levels were performed. At the end of the 30-day trial, the growth performance of tambaqui and plants was evaluated. Water quality was significantly (p < 0.05) affected by different flooding levels. Electrical conductivity and TDS decreased with an increasing in flooding levels. The flooding levels significantly influenced (p < 0.05) the concentration of total ammonia and nitrate between the treatments. The 5 cm flooding level showed the best plant development indexes for total height, aerial portion height, root height and aerial portion fresh mass. No significant differences (p < 0.05) were observed in tambaqui growth performance. The aquaponic system proved to be effective in reusing fish waste (excreta and feed leftovers) generated in the system. The biotransformation of waste into nutrients allowed the growth of plants and nitrifying bacteria, which, through their metabolic pathways, ensured the purification and reuse of water, avoiding the discharge of this waste into the environment. [ABSTRACT FROM AUTHOR]

Abstract Objective Single‐cell RNA‐sequencing of middle turbinate mucosa was performed to create the first single‐cell transcriptome catalog of this part of the human body. Study Design Basic science research. Setting Single center, tertiary care center. Methods Samples were obtained from the head of the middle turbinate from a healthy volunteer. After the specimen was prepared per lab protocol, cells were dissociated, resuspended, and counted. Single‐cell libraries were then prepared according to the 10x Genomics protocol and sequenced using NovaSeq 6000 (Illumina). Sequencing data were processed using Cell Ranger, and clustering and gene expression analysis was performed using Seurat. Cell types were annotated through expression profiling of single cells using known markers and data from other single‐cell studies. Results Fourteen unique cell types were identified, including serous, goblet, club, basal, ciliated, endothelial, and mesenchymal cells, as well as multiple types of blood cells. Conclusion This catalog provides a comprehensive depiction of the cellular composition of middle turbinate mucosa. By uncovering the cellular stratification of gene expression profiles in the healthy middle turbinate epithelium, the groundwork has been laid for further investigation into the molecular pathogenesis and targeted therapy of sinonasal disease.

Marumoside, Rutin and Quercetin are Moringa oleifera (MO) bioactive compounds which may act as anti-oxidant, anti-bacterial, anti-bone resorption, anti-inflammatory agent, pro-osteogenic and pro-growth factor that beneficial during bone remodeling. Aim: to analyze the Marumoside, Rutin and Quercetin of MO to Tartate Resistant Acid Phosphatase (TRAP), Nuclear Factor T-Cell (NFATc1), Nuclear Factor Kappa Beta (NFKB), Tumor Necrosis Factor Alpha (TNF-a), Heat Shock Protein (HSP)-70, HSP-10, receptor activator nuclear kappa beta and its ligand (RANK-RANKL), matrix metalloproteinase-9 (MMP-9), Peptidoglycan, Flagellin, Dectin, Runt Related Transcription Factor-2 (RUNX2), Osterix, Osteoprogetrin (OPG), Vascular Endothelial Growth Factor (VEGF), fibroblast growth factor-2 (FGF-2), collagen type 1 alpha-1 Coll1a1) through bioinformatics approach, an in silico study. Chemical compounds from MO used in this study consisted of Marumoside, Rutin and Quercetin obtained from PubChem database. The target proteins with 3D structures obtained from RCSB-PDB database. Canonical SMILE from MO compound was used to predict absorption, distribution, metabolism, excretion, toxicity (ADMET) consisting of physicochemical properties, water solubility, and drug-likeness, toxicity level based on LD50. Marumoside, rutin, and quercetin of MON act as drug-like molecules and toxicity were low. Quercetin has greatest negative binding energy and it was predicted to inhibit TRAP, NFATc1, NFKB, TNF-a, HSP-70, RANK, MMP-9, Peptidoglycan, Flagellin, Dectin and upregulate RUNX2, Osterix, HSP-10, RANKL, OPG, VEGF, FGF-2, Coll1a1 in silico. Conclusion Quercetin of MO is predicted to trigger inhibition activity of bone resorption, pro-inflammatory cytokines as well as triggering the increased activity of bone apposition, antioxidant, anti-inflammatory cytokine and growth factor. [ABSTRACT FROM AUTHOR]

Stem cells (SCs) have evolved as an interesting and viable factor in ophthalmologic patient care in the past decades. SCs have been classified as either embryonic, mesenchymal, tissue-specific, or induced pluripotent cells. Multiple novel management techniques and clinical trials have been established to date. While available publications are predominantly animal-model-based, significant material is derived from human studies and case-selected scenarios. This possibility of explanting cells from viable tissue to regenerate/repair damaged tissue points to an exciting future of therapeutic options in all fields of medicine, and ophthalmology is surely not left out. Adipose tissue obtained from lipo-aspirates has been shown to produce mesenchymal SCs that are potentially useful in different body parts, including the oculo-visual system. An overview of the anatomy, physiology, and extraction process for adipose-tissue-derived stem cells (ADSC) is important for better understanding the potential therapeutic benefits. This review examines published data on ADSCs in immune-modulatory, therapeutic, and regenerative treatments. We also look at the future of ADSC applications for ophthalmic patient care. The adverse effects of this relatively novel therapy are also discussed.