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Red spinach (Amaranthus tricolor L.) is a vegetable plant that contains vitamins A and C, mineral salts and antioxidants. Red spinach can be cultivated to produce a high vitamin and antioxidant content plant by providing shade so as to obtain optimal sunlight intensity. In this study, shading of various colors and percentages was carried out with the aim of regulating the entry of light wavelengths and the amount of sunlight intensity to be absorbed by plants, so it was known what wavelengths were absorbed by plants and their effect on ß-carotene content in red spinach. The equipment used in the study were meter, luxmeter, paranet, Leaf Area Meter (LAM), RHS color chart and the materials used were red spinach seeds of Mira variety, soil, water, goat manure and pearl NPK fertilizer. The experimental design used is a simple Randomized Group Design (RBD) method, consisting of 7 shade treatments, namely no shade and white, green, blue shades (50% and 85% density respectively). The treatment factors used different colors and levels of shade net density with observation parameters including plant height, number of leaves, leaf area, fresh weight of plants, fresh weight of consumption and leaf color. Observations were made at the age of the plants 10, 15, 20, 25 DAP. The data were analyzed by RBD and then analyzed by LSD test at 5% real level. Based on the researc, it is known that the combined treatment of blue shade color with a density of 50% gives the best results. [ABSTRACT FROM AUTHOR]

Textile industry waste such as methylene blue can pose a serious threat to health and ecosystems. One solution to overcome this problem is to utilize photocatalytic technology using TiO2 semiconductors. This research aims to make green synthetic TiO2 nanoparticles (NPs) using red spinach (Amaranthus Tricolor L.) leaf extract and used for photodegradation of methylene blue. Extraction of red spinach leaves showed that 30 min and a temperature of 95°C showed the highest phenolic content. In this research, several phenolic compounds were also identified in red spinach extract. For TiO2 NPs, the X-ray diffraction results show that TiO2 has a pure anatase phase. Electron microscope results show that TiO2 has the shape of agglomerated spherical clusters. The particle size distribution shows that TiO2 has an average size range of 77–90 nm. The methylene blue photodegradation showed that TiO2 had an efficiency of 90.36% for 180 min, higher than commercial TiO2 Evonic. This result can be attributed to the small particle size and broadband spectra so that photocatalysis is more effective.

Red spinach (Amaranthus cruentus), a rich source of antioxidant anthocyanins, is susceptible to light degradation. Foam mat drying (FMD) is a promising technique to preserve these pigments. This study compared the physicochemical properties and antioxidant activity of gels incorporating FMD and ethanolic extracts of A. cruentus (EEAC). Both extracts were incorporated into gels at varying concentrations (1%, 3%, and 5%). FMD-EEAC gels exhibited a darker red color and significantly higher total anthocyanin content (8.33 ± 0.25/100 g) and stronger antioxidant activity (IC50 of 35.67 ± 1.87 ppm) compared to EEAC gels (10.45 ± 0.15/100 g and IC50 of 47.88 ± 2.45 ppm, respectively). Both gel types had similar pH values (5.32-5.77). Increasing the concentration of either extract affected the viscosity, spreadability, and adhesion of the gels. Importantly, FMD-EEAC gels displayed significantly higher antioxidant activity (58.75 ± 2.12 to 64.72 ± 2.01%) than EEAC gels (31.75 ± 2.13 to 50.12 ± 3.01%) across all formulations. These findings suggest that FMD-based gels offer a superior delivery system for A. cruentus antioxidants, potentially leading to innovative food products with enhanced nutritional value and health benefits.

Limiting factors are very important for plant growth. The aim of environmental limiting factor research is to examine several limiting factors and identify how significant the role of limiting factors is in plant growth. The way the work is done is to observe 4 variations which will be given different treatment. The method used is an experimental study with quantitative analysis, that is, each existing data is processed in tables and explained. The results of this research show significant differences, namely variation A1 has a height of 6.9 cm and a number of leaves of 7 pieces, variation A2 has a height of 19.8 cm with a number of leaves of 72 pieces, variation A3 has a height of 5.3 cm and a number of leaves of 7 pieces., and the A4 variation has a height of 9.3 cm and a total of 24 leaves. Of all the variations carried out, it is proven that water, soil, air, temperature, and sunlight play an important role in plant growth. A policy recommendation that can be implemented is the use of screen houses. A screen house is a building made of plastic which functions to protect against pest attacks and supports direct sunlight exposure to plants. [ABSTRACT FROM AUTHOR]

Textile industry waste such as methylene blue can pose a serious threat to health and ecosystems. One solution to overcome this problem is to utilize photocatalytic technology using TiO2 semiconductors. This research aims to make green synthetic TiO2 nanoparticles (NPs) using red spinach (Amaranthus Tricolor L.) leaf extract and used for photodegradation of methylene blue. Extraction of red spinach leaves showed that 30 min and a temperature of 95°C showed the highest phenolic content. In this research, several phenolic compounds were also identified in red spinach extract. For TiO2 NPs, the X-ray diffraction results show that TiO2 has a pure anatase phase. Electron microscope results show that TiO2 has the shape of agglomerated spherical clusters. The particle size distribution shows that TiO2 has an average size range of 77–90 nm. The methylene blue photodegradation showed that TiO2 had an efficiency of 90.36% for 180 min, higher than commercial TiO2 Evonic. This result can be attributed to the small particle size and broadband spectra so that photocatalysis is more effective. [ABSTRACT FROM AUTHOR]

VP3 biofertilizer is a biological agent that has been formulated in previous research. It was applied once with compost and has been tested on red spinach and green spinach in the greenhouse to see its effect on yield and quality (chlorophyll content, anthocyanins, and shelf life at room temperature). Red spinach and green spinach had shown a significantly different growth response to applying biological fertilizers. VP3 biofertilizer with compost had the highest yield effect but was not significantly different from the recommended Nitrogen Phosphate Potassium (NPK) and Effective Microorganism4(EM4) treatment with compost. The application of VP3 Biofertilizer with NPK fertilizer showed a darker color (higher chlorophyll and anthocyanin content) than the application of biological fertilizers, but the values were not significantly different. The application of VP3 biofertilizer with compost affected the shelf life of both red spinach and green spinach. Spinach plants remained fresh at room temperature for three days of storage, while the other treatments lost freshness on the 3rd day after harvesting and even loosened the leaf on the 4th day after harvesting. Therefore, VP3 biofertilizer has potential to increase the yield and quality of spinach and other vegetable crops. The addition of the application period of VP3 biofertilizer needs to be carried out and observed to increase the yield and quality of plants further. Canonical Variate Analyses (CVA) can distinguish the grouping of treatments based on the selected parameter. Biplot assessment provides information on the strong relationship between the yield of green spinach and red spinach and a number of leaves, leaf area, and plant height. The application of VP3 biofertilizer with compost had a significant effect on the yield and quality and produced different effects between treatments, both in the case of green spinach and red spinach. [ABSTRACT FROM AUTHOR]

VP3 biofertilizer is a biological agent that has been formulated in previous research. It was applied once with compost and has been tested on red spinach and green spinach in the greenhouse to see its effect on yield and quality (chlorophyll content, anthocyanins, and shelf life at room temperature). Red spinach and green spinach had shown a significantly different growth response to applying biological fertilizers. VP3 biofertilizer with compost had the highest yield effect but was not significantly different from the recommended Nitrogen Phosphate Potassium (NPK) and Effective Microorganism4(EM4) treatment with compost. The application of VP3 Biofertilizer with NPK fertilizer showed a darker color (higher chlorophyll and anthocyanin content) than the application of biological fertilizers, but the values ​​were not significantly different. The application of VP3 biofertilizer with compost affected the shelf life of both red spinach and green spinach. Spinach plants remained fresh at room temperature for three days of storage, while the other treatments lost freshness on the 3rd day after harvesting and even loosened the leaf on the 4th day after harvesting. Therefore, VP3 biofertilizer has potential to increase the yield and quality of spinach and other vegetable crops. The addition of the application period of VP3 biofertilizer needs to be carried out and observed to increase the yield and quality of plants further. Canonical Variate Analyses (CVA) can distinguish the grouping of treatments based on the selected parameter. Biplot assessment provides information on the strong relationship between the yield of green spinach and red spinach and a number of leaves, leaf area, and plant height. The application of VP3 biofertilizer with compost had a significant effect on the yield and quality and produced different effects between treatments, both in the case of green spinach and red spinach.

Fish color brightness is influenced by food intake, but no prior reviews have explored the use of red spinach extract in fish feed to enhance color brightness and promote growth in guppy (Poecilia reticulata). Therefore, this study aims to determine the suitable concentration of red spinach extract Amaranthus tricolor color brightness, growth, and survival of guppy. The experiment followed a completely randomized design with three concentrations of red spinach extract (3%, 4%, and 5%) replicated three times at the UPTD Fish Breeding Center in North Binjai Regency, North Sumatra Province. Furthermore, red spinach extract was mixed with the commercial diet containing 41% crude protein and administered to fish at a rate of 3% of their body weight daily for 40 days. The results showed a significant effect of red spinach extract doses on guppy color brightness (P<0.05), but no significant influence on weight gain and fish survival (P>0.05). It was also discovered that the average color increase was 2,652 in feed mixed with 5% red spinach extract. Similarly, the highest fish weight gain was observed at the 5% concentration, which did not significantly differ from the other concentrations. Fish survival rates were high, with no significant differences among the treatments. Therefore, it was concluded that the optimal concentration of red spinach extract for enhancing guppy color brightness was 5%. [ABSTRACT FROM AUTHOR]

Background and aim: Indonesia is one of the countries with the highest incidence of type 2 diabetes in the world. Management of diabetes in management of food that does not cause depression in patients and is rich in nutrients as a consequence of consumption drug routine is things to do searching for Street exit. Study this aim for With Thus, we explore the effect of rice red and spinach red in the form of cookies to index glycemic and haemoglobin levels. Methods: as many as 50 respondents diagnosed with type 2 DM with a history of more than two years registered in a quasi-experimental test pre-pest design with the control group in Sorong, West Papua. Cookies consisting of 200 grams of flour rice red mixed with 20 grams of flour spinach red were given for 30 days. Inspection Check blood sugar and haemoglobin levels Before and after the intervention. Data analysis used an independent sample t-test. Results: we found that occur drop index glycemic as big as 81.8 Mg/dl and increased haemoglobin by 1.161 g/dl in the group intervention Conclusions: Rice red and spinach red in the form of cookies show benefit potential at index glycemic and haemoglobin levels among participants with diabetes mellitus type 2. [ABSTRACT FROM AUTHOR]

The red velvet that many people like is a pastry food that is usually found in restaurants and cafes. Red Velvet, which usually uses red food coloring, in this study replaced it with red beet and spinach coloring. Beets, which have high nutrition, are very useful and suitable to be processed into natural dyes in the home industry that are easy to process. One of the products to apply it is Red Velvet cake. Red Velvet cake basically uses a mixture of food coloring. This research tries to replace red food coloring by utilizing beet and red spinach which are very good for the body. This study aims to provide a new alternative to natural dyes in the making of Red Velvet cake. Experimental research was carried out by conducting systematic and planned experiments and tests on Red Velvet cake and collecting primary and secondary data as well. All data were presented descriptively with SPSS. From the mean results, it can be concluded that the Red Velvet Cake using red beet and red spinach dye is acceptable to the public.

Red spinach (Amaranthus tricolor L.) contains various secondary metabolites that act as antioxidants. These activities can be used to maintain the health of facial skin due to the negative impact of free radicals in the form of cosmetic preparations of peel-off gel mask. This study aims to make a peel-off gel mask preparation from red spinach extract that meets the requirements for physical evaluation of the preparation. The extraction process was carried out by maceration method using 96% ethanol as solvent. The thick extract was then made into a peel-off gel mask preparation with variations in extract concentration of 0.5% (F1), 1% (F2), and 2% (F3). Furthermore, physical evaluation of the preparation at room temperature for 4 weeks of storage, drying time test, irritation test, preference test, and cycling test was carried out. The red spinach used has been determined as Amaranthus tricolor L. The results of phytochemical screening of the thick extract showed that the extract contained positive alkaloids, saponins and flavonoids. The results of the evaluation showed that all preparations met the physical evaluation requirements and were not irritating. The higher the concentration of the extract, the darker the color of the preparation and the higher the pH and viscosity values. The preparation was stable during storage at room temperature but decreased in pH value and dispersion after passing 6 cycles of cycling test. The results of the preference test showed 60% of respondents liked F3, which is a formula with a dark green color, a distinctive aroma of red spinach, homogeneous, pH 7.54, dispersion of 6.2 cm, the viscosity of 7200 cps and drying time of 20.23 minutes.

The synthesis of few-layer graphene from graphite typically uses N, N methyl pyrrolidone (NMP) or dimethylformamide (DMF) due to the strong affinity of both solvents for graphite. However, NMP and DMF are known as carcinogens and a long-time exposure to these substances may subject users to potential risk of major health issue later. Therefore, a replacement with dispersing solvent that is not only harmless but also able to exfoliate graphite at an excellent concentration yield must be outlined for a sustainable mass-production of graphene. In this work, we have successfully exfoliated graphite to few-layer graphene with a recorded yield concentration of up to 0.75°mg/ml (2.5°h) just by using extracted red spinach/water mixture as an exfoliating medium. The prepared graphene was found to possess less structural defect (I D /I G : 0.5) and high C/O ratio (6.8) and can be used further as an electrical conductive ink for smart "Sticky Note" sensor. The fabricated device was able to detect strain and temperature with gauge factor and temperature coefficient resistance of 23.5 and −32.14 × 10-4°Ω/°;C, respectively. We believe that this study would be useful for the preparation of environmental-friendly graphene that is not only strain and thermally sensitive but also producible at low -cost. Image 1 [ABSTRACT FROM AUTHOR]

Background: Postpartum mothers are susceptible to anemia, due to loss of blood count during labor. Postpartum anemia causes uterine subinvolution which causes postpartum hemorrhage, facilitates puerperium infection, decreases breastfeeding and easy mamae infection. One alternative to prevent anemia in postpartum is to consume vegetables with high iron one red spinach. Red spinach is one of the non-heme iron sources needed to synthesize hemoglobin. Objective: To determine the effect of red spinach in increasing the levels of hemoglobin in postpastum mothers. Methods: This was a quasy experiment with pretest posttest with control group design in October-December 2017 at the Community Health Center of PONED Tarub, Tegal regency. Thirty postpartum mothers were included using purposive sampling, which 15 assigned in each group. Blood sampling and examination were performed in each respondent. Paired and Independent t-test were done for data analysis. Results: The results of the 14-day intervention of given red spinach extract showed that there was a significant increase of hemoglobin level (1.25 g / dL) with p-value 0.047. Conclusion: There is a significant effect of consuming red spinach extract (amaranthus tricolor L.) on the increase of hemoglobin level in postpartum mother. It is recommended that midwives could apply this intervention and cooperate with agriculture and food processing in order to produce red spinach extract that increases hemoglobin, hematocrit and erythrocytes levels.

Antioxidants in red spinach (Amaranthus gangeticus) extract are proposed to combat the toxicity of lead acetate in the central nervous system. The effect of red spinach extract to the morphology of post weaning mouse hippocampus model (Mus musculus) in pregnant mice that received oral lead acetate during pregnancy was analyzed. Twenty seven post-weaning mice offsprings were grouped into 3 groups: M0 (sodium carboxymethyl cellulose or CMC Na 0.5%), M1 (CMC Na 0.5%+lead acetate 1.16 mg/10 g of body weight/day) and M2 (CMC Na 0.5%+lead acetate 1.16 mg/10 g of body weight/day+red spinach extract 382.2 mg/10 g of body weight/day). Brains were histologically processed and stained with hematoxylin-eosin (HE). Width of stratum granulare (SG) of the dentate gyrus (GD) and each of stratum oriens-pyramidale (SOP) of CA1, CA2, CA3 from each mouse hippocampus were obtained and analyzed statistically using analysis of variance (ANOVA) and least significant difference (LSD) (p0.05). In conclusion, red spinach extract might dampen the adverse effects of oral lead acetate in post-weaning mouse hippocampus model from pregnant mice orally exposed to lead acetate during pregnancy.

Betacyanins are reddish to violet pigments that can be found in red pitahaya (Hylocereus polyrhizus) and red spinach (Amaranthus dubius). This study investigated the impact of sub-fractionation (solvent partitioning) on betacyanin content in both plants. Characterization of betacyanins and evaluation of their antimicrobial activities were also carried out. Betanin was found in both plants. In addition, isobetanin, phyllocactin and hylocerenin were found in red pitahaya whereas amaranthine and decarboxy-amaranthine were found in red spinach. Sub-fractionated red pitahaya and red spinach had 23.5 and 121.5 % more betacyanin content, respectively, than those without sub-fractionation. Sub-fractionation increased the betanin and decarboxy-amaranthine content in red pitahaya and red spinach, respectively. The betacyanin fraction from red spinach (minimum inhibitory concentration [MIC] values: 0.78-3.13 mg/mL) demonstrated a better antimicrobial activity profile than that of red pitahaya (MIC values: 3.13-6.25 mg/mL) against nine Gram-positive bacterial strains. Similarly, the red spinach fraction (MIC values: 1.56-3.13 mg/mL) was more active than the red pitahaya fraction (MIC values: 3.13-6.25 mg/mL) against five Gram-negative bacterial strains. This could be because of a higher amount of betacyanin, particularly amaranthine in the red spinach. [ABSTRACT FROM AUTHOR]

Introduction: Inorganic nitrate ingestion has been posited to affect arterial blood pressure and vascular function. Purpose: We sought to determine the acute effect of a red spinach extract (RSE) high in inorganic nitrate on vascular reactivity 1-h after ingestion in peripheral conduit and resistance arteries. Methods: Fifteen ( n = 15; males 8, females 7) apparently healthy subjects (aged 23.1 ± 3.3 years; BMI 27.2 ± 3.7 kg/m) participated in this crossover design, double-blinded study. Subjects reported to the lab ≥2-h post-prandial and consumed RSE (1000 mg dose; ~90 mg nitrate) or placebo (PBO). Venipuncture was performed on three occasions: baseline, 30-min post-ingestion and between 65 to 75-min post-ingestion. Baseline vascular measurements [i.e., calf venous occlusion plethysmography, brachial artery flow-mediated dilation (FMD)], 30-min of continuous blood pressure (BP) and heart rate (HR) analysis, and follow-up vascular measurements beginning at 40-min post-ingestion were also performed. Results: Humoral nitrate following RSE ingestion was significantly higher at 30- (+54 %; P = 0.039) and 65 to 75-min post-ingestion compared to baseline (+255 %, P < 0.001) and PBO at the same time points ( P < 0.05). No significant changes in BP or HR occurred in either condition. Peak reactive hyperemia (RH) calf blood flow increased significantly (+13.7 %; P = 0.016) following RSE ingestion, whereas it decreased (−14.0 %; P = 0.008) following PBO ingestion. No significant differential FMD responses were detected ( P > 0.05), though RH was decreased following the baseline measure in both conditions. Conclusions: RSE significantly increased plasma nitrate 30-min post-ingestion, but acute microvascular (i.e., resistance vasculature) reactivity increases were isolated to the lower limb and no appreciable change in brachial artery FMD was observed. [ABSTRACT FROM AUTHOR]

In this work, natural dye (anthocyanin) was extracted from red spinach (Amaranthus dubius) and refined by solvent extraction process which was used as a sensitizer to enhance the overall cell performance of the prepared DSSC. Unwanted impurities have been separated with the organic layer from the bottom of the separatory funnel and refined anthocyanin dye extract was collected from the aqueous layer. Extract of crude red spinach dye-sensitized DSSCs have shown the cell parameters of short circuit current (I sc) = 0.642 mA, open-circuit voltage (V oc) = of 0.329 V, fill factor (FF) = 0.434 and efficiency= 0.091%. For refined anthocyanin dye extract, the photoelectrical properties of DSSCs were recorded as, I sc varied from 0.642 mA to 1.385 mA, V oc ranged from 0.329 V to 0.379 V, and FF ranged from 0.434 to 0.469. DSSC sensitized with purified anthocyanin dye showed an efficiency of 0.247%, which was almost 2.7 times higher than that of the crude red spinach extract (without refinement) sensitized DSSC's cell conversion efficiency. Finally, degradation in the cell efficiency of crude and refined anthocyanin dye based DSSCs were measured after 48 h. • For dye refinement, the solvent-solvent extraction method was used for dye extracted from red spinach (Amaranthus dubius). • DSSC sensitized by crude natural dye yields only 0.091%; however, DSSC sensitized by refined natural dye yields 0.247%. • After 48h aging, crude red spinach extract-based DSSC's cell performance degrades about 62.6%. • After 48h aging, refined red spinach extract-based DSSC's cell performance degrades about 41.7%. [ABSTRACT FROM AUTHOR]

The objective of this study was to determine the anti cancer effects of red spinach Amaranthus gangeticus Linn) in vitro and in viva. For in vitro study, microtitration cytotoxic assay was done using 3-(4,5-dimethylthiazol-2-il)-2,5-diphenil tetrazoliurn bromide (MTT) kit assay. Results showed that aqueous extract of A gangeticus inhibited the proliferation of liver cancer cell line (HepG2) and breast cancer cell line (MCF-7). The IC50 values were 93.8 μg/ml and 98.8 μg/ml for HepG2 and MCF-7, respectively. The inhibitory effect was also observed in colon cancer cell line (Caco-2), but a lower percentage compared to HepG2 and MCF-7. For normal cell line (Chang Liver), there was no inhibitory effect. In the in vivo study, hepatocarcinogenesis was monitored in rats according to Solt and Farber (1976) without partial hepatectomy. Assay of tumour marker enzymes such as glutathione S-transferase (GST), gamma-glutamyl transpeptidase (GGT), uridyl diphosphoglucuronyl transferase (UDPGT) and alkaline phosphatase (ALP) were carried out to determine the severity of hepatocarcinogenesis. The result found that supplementation of 5%, 7.5% and 10% of A. gangeticus aqueous extract to normal rats did not show any significant difference towards normal control (P <0.05). The exposure of the rats to chemical carcinogens diethylnitrosamine (DEN) and 2- acetylaminofluorene (AAF) showed a significant increase in specific enzyme activity of GGT, GST, UDPGT and ALP compared to normal control (P <0.05). However, it was found that the supplementation of A. gangeticus aqueous extract in 5%, 7.5% and 10% to cancer-induced rats could inhibit the activity of alt tumour marker enzymes especially at 10% (P <0.05). Supplementation of anti cancer drug glycyrrhizin at suggested dose (0.005%) did not show any suppressive effect towards cancer control (P <0.05). In conclusion, A. gangeticus showed anticancer potential in in vitro and in vivo studies. [ABSTRACT FROM AUTHOR]

Background: Dietary supplements purported to increase circulating nitric oxide are very popular among consumers. We determined the acute impact of two novel dietary supplements on plasma nitrate/nitrite (NOx) and nitrite alone. Methods: 20 men and women (age: 24 ± 5 years) ingested two different nitrate-rich supplements (Resync Recovery Blend at 7.5 g and 15 g; Resync Collagen Blend at 21 g), or placebo, on four different days. Fasting blood samples were obtained before and 75 min following ingestion and analyzed for NOx and nitrite. Results: Nitrite was not differently impacted by treatment (p > 0.05). The NOx response for men and women was very similar, with no sex interactions noted (p > 0.05). Condition (p < 0.0001), time (p < 0.0001), and condition x time (p < 0.0001) effects were noted for NOx. Values increased from baseline to post-ingestion for the Resync Recovery Blend at 7.5 g (11 ± 9 to 101 ± 48 µM) and at 15 g (9 ± 5 to 176 ± 91 µM), as well as for the Resync Collagen Blend (9 ± 9 to 46 ± 21 µM), while values for placebo remained stable (9 ± 7 to 8 ± 5 µM). Conclusion: While nitrite alone was not impacted by treatment, both Resync products result in an increase in plasma NOx, with the increase proportionate to the quantity of "nitric oxide blend" ingredients contained within each product. Future studies are needed to determine the physiological implications of the increased NOx, as pertaining to exercise performance and recovery, in addition to other aspects of human health. [ABSTRACT FROM AUTHOR]

Objective: Nitric oxide (NO) is one of the most important signaling molecules produced within the body. Continuous generation of NO is essential for the integrity of the cardiovascular system. The aim of this study was to assess whether oral intake of a nitrate (NO3-)-rich dietary supplement (amaranth extract) is able to increase NO3- and nitrite (NO2-) levels in blood plasma and saliva of healthy adults., Methods: In the present study, bioavailability and pharmacokinetics of NO3- and NO2- from amaranth extract (2 g as single dose) was studied in 16 healthy individuals and compared with placebo in a crossover design. The NO3- and NO2- levels in plasma as well as saliva were measured up to 24 h., Results: After administration of amaranth extract, the NO3- levels in plasma as well as saliva were found to be significantly (P < 0.001) higher than in the placebo group. The NO2- level in plasma was slightly higher (P < 0.05) in the amaranth group (test group) compared with that in the placebo group, whereas the saliva NO2- level was significantly high (P < 0.001) in the amaranth extract-treated group than the placebo group., Conclusions: These results clearly indicate that a single oral dose of amaranth extract is able to increase the NO3- and NO2- levels in the body for at least 8 h. The increase in NO3- and NO2- levels can help to improve the overall performance of people involved in vigorous physical activities or sports., (Copyright © 2016 Elsevier Inc. All rights reserved.)