Search

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:45:37Z No. of bitstreams: 1 S0074-02762010000300004.pdf: 1219265 bytes, checksum: 13239bf33cbaa540276f11cc07374c16 (MD5) Made available in DSpace on 2013-08-22T18:45:37Z (GMT). No. of bitstreams: 1 S0074-02762010000300004.pdf: 1219265 bytes, checksum: 13239bf33cbaa540276f11cc07374c16 (MD5) Previous issue date: 2010-05-01 Made available in DSpace on 2013-09-30T19:34:44Z (GMT). No. of bitstreams: 2 S0074-02762010000300004.pdf: 1219265 bytes, checksum: 13239bf33cbaa540276f11cc07374c16 (MD5) S0074-02762010000300004.pdf.txt: 30503 bytes, checksum: d6f0b5a4a1f020d070e9ab33f3f9b953 (MD5) Previous issue date: 2010-05-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:50:42Z No. of bitstreams: 2 S0074-02762010000300004.pdf: 1219265 bytes, checksum: 13239bf33cbaa540276f11cc07374c16 (MD5) S0074-02762010000300004.pdf.txt: 30503 bytes, checksum: d6f0b5a4a1f020d070e9ab33f3f9b953 (MD5) Made available in DSpace on 2014-05-20T13:50:42Z (GMT). No. of bitstreams: 2 S0074-02762010000300004.pdf: 1219265 bytes, checksum: 13239bf33cbaa540276f11cc07374c16 (MD5) S0074-02762010000300004.pdf.txt: 30503 bytes, checksum: d6f0b5a4a1f020d070e9ab33f3f9b953 (MD5) Previous issue date: 2010-05-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Experimental autoimmune encephalomyelitis (EAE) is mediated by CD4+ Th1 cells that mainly secrete IFN-γ and TNF-α, important cytokines in the pathophysiology of the disease. Spontaneous remission is, in part, attributed to the down regulation of IFN-γ and TNF-α by TGF-β. In the current paper, we compared weight, histopathology and immunological parameters during the acute and recovery phases of EAE to establish the best biomarker for clinical remission. Female Lewis rats were immunised with myelin basic protein (MBP) emulsified with complete Freund's adjuvant. Animals were evaluated daily for clinical score and weight prior to euthanisation. All immunised animals developed the expected characteristics of EAE during the acute phase, including significant weight loss and high clinical scores. Disease remission was associated with a significant reduction in clinical scores, although immunised rats did not regain their initial weight values. Brain inflammatory infiltrates were higher during the acute phase. During the remission phase, anti-myelin antibody levels increased, whereas TNF-α and IFN-γ production by lymph node cells cultured with MBP or concanavalin A, respectively, decreased. The most significant difference observed between the acute and recovery phases was in the induction of TNF-α levels in MBP-stimulated cultures. Therefore, the in vitro production of this cytokine could be used as a biomarker for EAE remission. Universidade Estadual Paulista Instituto de Biociências Departamento de Patologia Universidade Estadual Paulista Instituto de Biociências Departamento de Patologia

Levels of mRNA and protein encoded by the TATA-binding protein (tbp) gene are shown to increase dramatically during late spermatogenesis in rodents, culminating in a highly testis-enriched expression pattern. Whereas adult spleen and liver contained roughly 0.7 and 2.3 molecules of TBP mRNA per haploid genome-equivalent, respectively, adult testis contained 80-200 molecules of TBP mRNA per haploid genome-equivalent. Comparison of nuclear and cytoplasmic levels of TBP mRNA in liver and testis suggested that nuclear events (transcription or processing) contribute roughly 12-fold, and cytoplasmic events (mRNA stability) roughly 6-fold, to testis-specific overaccumulation. Levels of nuclear TBP protein in testis cells were, on average, 8- and 11-fold higher than those in liver and spleen cells, respectively. Overexpression of TBP mRNA in testis began about 20 days after birth and reached a plateau around day 40, corresponding to the developmental emergence of haploid cells. Besides TBP, two other components of the general RNA polymerase II machinery, TFIIB and RNA polymerase II, were also overexpressed in testis. By immunostaining, it was found that TBP and RNA polymerase II were particularly rich in round spermatid nuclei. Our results suggest a molecular explanation for how early spermatids are able to accumulate all of the mRNA necessary for the final week of spermiogenesis.