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Dogs are susceptible to a diverse spectrum of dermatologic conditions, several of these skin disorders are analogous to dermatologic conditions occurring in humans. Pool toes appears on the plantar surface of the feet and toes; it is an aquatic pool-associated frictional dermatosis that results from repeated rubbing of the feet and toes against the rough cement at the bottom of the pool or its border. A frictional dermatosis that can develop on the uncalloused paw pads of puppies, resulting from repeated rubbing of the paws against ambient temperature, not heated, paved road is pavement paws. Neither pool toes nor pavement paws result from contact with a hot surface; these dermatoses are distinctive from beach feet, a thermal-associated superficial burn to the skin resulting from the hot temperature of the sand that contacts that the plantar feet and toes. In conclusion, similar to pool toes, the pathogenesis of pavement paws is a frictional dermatosis that can develop on the uncallused paw pads of puppies resulting from repeated rubbing of the paws against ambient temperature, not heated, pavement.

Background: Autosomal recessive ichthyosis leads to structural or biochemical changes that impair skin barrier function. Hypothesis/Objectives: To assess (1) the phenotype and genotype in a litter of Jack Russell Terriers with autosomal recessive congenital ichthyosis (ARCI), and (2) the defective skin barrier and determine if a topical ceramide can modulate the barrier. Animals: A healthy dam and litter of Jack Russell Terrier puppies (healthy male, affected male and female), one affected adult Jack Russell Terrier and one unrelated healthy Jack Russell Terrier. Materials and Methods: A severe cornification defect was identified via examination of affected puppies. As the phenotype worsened, the affected puppies received a topical application of ω‐0‐acylceramide for 10 days. Before humane euthanasia, the skin barrier was evaluated via transepidermal water loss (TEWL), corneometry and pH in affected dogs. Genomic testing was performed, and skin samples were analysed by light and electron microscopy. Results: Affected puppies were homozygous for the 1980 bp LINE‐1 insertion in the TGM1 (transglutaminase 1) gene; the unaffected littermate and the dam were heterozygous carriers. ARCI puppies were underweight and had a severe hyperkeratotic phenotype that impaired mobility. TEWL was markedly higher in affected dogs. The cutaneous pH of affected puppies was higher than the normal littermate. Treatment of the skin with ω‐0‐acylceramide normalised the pH to match the littermate and decreased TEWL. Electron microscopy revealed marked attenuation of the cornified envelope. Conclusions and Clinical Relevance: Dogs with TGM1‐deficient ARCI have an impaired skin barrier. Topical therapy can partially repair the barrier defect. [ABSTRACT FROM AUTHOR]

Urinary N-acetyl-β-D-glucosaminidase (NAG) activity is associated with kidney disease. In our study, we focused on evaluating the ratio of NAG to the urinary creatinine concentration (NAG/Cr) in the German Shepherd breed. Sixty-two healthy dogs and thirteen dogs with chronic kidney disease (CKD) were examined. The healthy dogs were divided into groups based on their sex and age (puppies and adolescent dogs under 2 years, adult dogs from 2 to 6 years and seniors over 6 years), while the dogs with CKD were divided based on the disease stage (CKD stage 1 and 2). No significant difference was detected between the males and females. Regarding the age dependence, significantly higher values were found in dogs older than 6 years (P = 0.020 3) compared to dogs aged 2-6 years. When comparing the healthy dogs to the dogs with CKD, the lowest NAG/Cr ratio was observed in the dogs with CKD stage 2. The reference interval for NAG/Cr in the German Shepherd was established in the range of 0.78-7.86 U/gCr. We observed no correlations with the commonly used markers of kidney disease such as creatinine, urea, or symmetrical dimethylarginine. Nevertheless, we encourage the further investigation of NAG in relation to CKD, taking the breed, sex and age of the patients into account. [ABSTRACT FROM AUTHOR]

Background: L. Australis is one of the most prevalent Leptospira strains infecting dogs, leading, in natural conditions, to severe life-threatening cases. Objective: The objective was to evaluate the onset and duration of immunity (OOI and DOI) induced by a new licensed quadrivalent antileptospiral vaccine (EURICAN® L4) including four Leptospira components (Canicola, Icterohaemorrhagiae, Grippotyphosa and Australis) against L. Australis. To this end, a severe L. Australis challenge model was developed, using a canine strain recently isolated from the field. Material and Methods: Seven- to ten-week-old puppies received two doses of the vaccine four weeks apart and were challenged with an L. Australis isolate two weeks (OOI) and 12 months (DOI) later. Mortality, clinical signs, leptospiremia, leptospiruria, renal carriage, and renal lesions were assessed after challenge. Results: The challenge induced multiple severe clinical signs in controls, leading to the death or euthanasia of 83% of puppies and 57% of adults. In controls, leptospiremia was detected in all dogs, leptospiruria in 67% of puppies and 86% of adults, kidneys tested positive for Leptospira in 83% of puppies and 71% of adults, and kidney lesions were observed in 100% of puppies and 86% of adults. In addition, thrombocytopenia associated with increased concentrations of urea, creatinine, and aspartate aminotransferase was recorded in controls displaying severe clinical signs. In both OOI and DOI studies, none of the vaccinates had clinical signs, no Leptospira was detected in blood, urine, and kidney samples, and no kidney lesions were observed in vaccinates. No significant changes in hematological and biochemical parameters in vaccinates were recorded. Conclusion: EURICAN® L4 was shown to induce quick and long-lasting protection against a severe L. Australis infectious challenge, preventing mortality, clinical signs, infection, bacterial excretion, renal lesions, and renal carriage. [ABSTRACT FROM AUTHOR]

Simple Summary: The increase in respiratory infections, particularly acute pneumonia, in puppies poses a significant health risk due to the disease's rapid severity. This study successfully identified four multidrug-resistant strains of extraintestinal pathogenic Escherichia coli from puppies with acute pneumonia, which contained various virulence genes and were classified using sequence typing. This study emphasizes the need to understand the molecular traits of Escherichia coli strains causing canine pneumonia. Such an understanding facilitates the formulation of targeted intervention strategies and the exploration of novel therapeutic approaches for the effective treatment of acute pneumonia in puppies. To determine the etiological agents responsible for acute pneumonia in puppies in China, this study utilized bronchoalveolar lavage (BAL) fluid extraction to enable the isolation, culture, biochemical identification, and 16S rRNA PCR amplification of the pathogens. Following preliminary identification, the pathogens underwent analysis for antibiotic resistance phenotypes and resistance genes. Additionally, the study examined the presence of virulence genes, conducted multilocus sequence typing (MLST), and performed whole-genome sequencing (WGS). The findings revealed that all four isolated pathogens were characterized as extraintestinal pathogenic Escherichia coli (ExPEC). The examined ExPEC strains demonstrated resistance to cephalosporins, tetracyclines, and penicillins, while remaining susceptible to aminoglycosides, beta-lactamase inhibitors, carbapenems, chloramphenicols, and sulfonamides. An analysis of virulence genes identified the presence of eight genes, namely CNF-I, fyuA, fimC, papC, ompA, fimH, irp2, and iroN, which are implicated in their invasiveness and potential to inflict tissue damage. The MLST analysis revealed that all ExPEC strains were classified under either sequence type ST131 (Achtman database) or ST43 (Pasteur database). The study further determined that these strains were absent in the kennel's drinking water source, thereby ruling out water contamination as a potential factor in the emergence of ST131-type ExPEC. This study offers a theoretical framework and empirical evidence for elucidating the potential pathogenic mechanisms and clinical therapeutic strategies of ExPEC in the etiology of acute pneumonia in puppies. [ABSTRACT FROM AUTHOR]

AbstractIn a retrospective metatranscriptomics study, we identified tick-borne encephalitis virus (TBEV) to be the causative agent for a fatal non-suppurative meningoencephalitis in a three-week-old Dalmatian puppy in Switzerland. Further investigations showed that the two other littermates with similar signs and pathological lesions were also positive for TBEV. By using an unbiased approach of combining high-throughput sequencing (HTS) and bioinformatics we were able to solve the etiology and discover an unusual case of TBEV in three young puppies. Based on our findings, we suggest that a vector-independent transmission of TBEV occurred and that most likely an intrauterine infection led to the severe and fulminant disease of the entire litter. We were able to demonstrate the presence of TBEV RNA by in situ hybridization (ISH) in the brain of all three puppies. Furthermore, we were able to detect TBEV by RT-qPCR in total RNA extracted from formalin-fixed and paraffin embedded (FFPE) blocks containing multiple peripheral organs. Overall, our findings shed light on alternative vector-independent transmission routes of TBEV infections in dogs and encourage veterinary practitioners to consider TBEV as an important differential diagnosis in neurological cases in dogs.

In human infants, the ability to show gaze alternations between an object of interest and another individual is considered fundamental to the development of complex social-cognitive abilities. Here we show that well-socialised dog puppies show gaze alternations in two contexts at an early age, 6–7 weeks. Thus, 69.4% of puppies in a novel object test and 45.59% of puppies during an unsolvable task alternated their gaze at least once between a person's face and the object. In both contexts, the frequency of gaze alternations was positively correlated with the duration of whimpering, supporting the communicative nature of puppies' gazing. Furthermore, the number of gaze alternations in the two contexts was correlated, indicating an underlying propensity for gazing at humans despite likely different motivations in the two contexts. Similar to humans, and unlike great apes or wolves, domestic dogs show gaze alternations from an early age if they are well-socialised. They appear to have a genetic preparedness to communicate with humans via gaze alternations early in ontogeny, but they may need close contact with humans for this ability to emerge, highlighting the interactive effects of domestication and environmental factors on behavioural development in dogs. [ABSTRACT FROM AUTHOR]

Simple Summary: Campus-based dog programs can reduce stress and enhance wellbeing in university students and staff. However, most research has focused on adult dogs, who are well-trained and accompanied by a handler. In our study, participants came to the university campus for 20 min interactions with an assistance dog puppy in training and their handler during one visit, and the handler alone in another visit. We asked participants about their stress and vitality levels before and after each interaction. Stress was lower after the interaction with the puppy and handler compared to the handler alone. Vitality was greater after both visits among participants who had their session with the puppy and handler first. This study indicates that assistance dog puppies in training, with knowledgeable handlers, can be effective in campus-based visitation dog programs. Brief interactions with a well-behaved dog can enhance wellbeing, but most campus-based dog visitation programs employ adult, trained dogs. There is little research about the impact of puppies on wellbeing or stress in this context. The aim of this study was to examine changes in perceived stress and vitality after interacting with a puppy. Staff and students (N = 32) at an Australian university attended the campus on two occasions, one week apart, as part of a crossover design. Participants were pseudo-randomly allocated to a group whereby they spent 20 min interacting with a handler alone at Visit 1 and interacted with a puppy and handler at Visit 2, or another group which reversed the interaction order. Perceived stress and subjective vitality were measured before and after each interaction. The increase in vitality was greater in the group experiencing the puppy and handler interaction first (significant main effect, F(1,49) = 646.89, p = 0.024, η2p = 1.00), regardless of the visit, possibly due to a social lubricating effect by the puppy, which carried over to the 'handler alone' second visit. Reductions in perceived stress were greater after the interaction with the puppy, for both groups (significant interaction effect, F(1,49) = 5.13, p = 0.029, η2p = 0.11), indicating that the puppy's presence can reduce stress more than the handler alone. This extends the evidence for university-based dog-facilitated wellbeing programs, by showing that interactions with puppies can also be effective. This is important as it may mean that puppies already on campus as part of a socialization/training program can be incorporated into wellbeing programs for staff and students. [ABSTRACT FROM AUTHOR]

Generalized hair loss is a problem associated with the loss of the entire coat and is one of the main causes of veterinary visits to animals. In most cases, it is an acquired condition, although there are also congenital forms of hair loss, caused by abnormal development of hair follicles. This article discusses both types of causes of hair loss in dogs. Congenital hair loss can occur in the first weeks of puppies' life, but it does not progress with age. Although not always, most often congenital hair loss has a genetic basis. Dysplasia with bleaching of hair color and follicular dysplasia of black hair are examples of congenital skin neuroectodermal disorders. Acquired hair loss is usually caused by hypothyroidism, hyperadrenocorticism and hyperestrogenism, and occurs in dogs at a later age associated with hormonal disorders. [ABSTRACT FROM AUTHOR]

The study conducted in Morocco focused on addressing the challenges posed by canine parvovirus (CPV-2) through comprehensive research, vaccine development, and efficacy assessment. Through real-time PCR screening and genotyping, CPV-2 variants were identified circulating in the region. An inactivated vaccine, derived from a CPV-2 strain isolated from a symptomatic dog, was produced and evaluated for safety and efficacy. The vaccine, from the strain named "CaPV M/3-2022", demonstrated safety in vaccinated puppies, with no adverse reactions observed during the trial period. Efficacy trials showed that vaccinated puppies remained healthy and exhibited lower viral excretion post-challenge compared to unvaccinated controls. These results indicate that the vaccine effectively protects against illness related to CPV-2 and reduces viral shedding. The study provides valuable insights into CPV-2 epidemiology in Morocco, offers a promising vaccine solution, and underscores the importance of vaccination in controlling CPV-2 outbreaks and protecting canine health. [ABSTRACT FROM AUTHOR]

Background: Microbial colonization during early life has a pivotal impact on the host health, shaping immune and metabolic functions, but little is known about timing and features of this process in dogs. The objectives of this study were to characterize the first step of intestinal microbiota development in naturally delivered canine puppies and to investigate its relationship with the maternal bacterial flora, using traditional culture and molecular analyses. Sixty puppies of two breeds, Appenzeller Cattle Dog (n = 3 dams) and Lagotto Romagnolo (n = 6), housed in the same breeding kennel, were included in the study. Swabs were collected in duplicate (for culture and for molecular analysis) from the dams' vagina and rectum at the end of parturition, from puppies' rectum, before maternal care, and from the environment (floor of the nursery and parturition box). Results: 93.3% meconium samples showed bacterial growth, limited to a few colonies in 57.0% of cases. High growth was detected for Enterococcus faecalis, which was the most frequently isolated bacterium. The genus Enterococcus was one of the most represented in the dams' rectum and vagina (88.9% and 55.6%, respectively). The genera Staphylococcus, Enterococcus, Escherichia and Proteus were also often isolated in meconium but were usually present in maternal samples as well, together with ubiquitous bacteria (Acinetobacter, Psychrobacter). In the environmental samples, just a few bacterial species were found, all with low microbial load. Additionally, bacteria of the phyla Proteobacteria, Firmicutes, and Actinobacteria were identified in meconium through molecular analysis, confirming the culture results and the early colonization of the newborn gut. Maternal, meconium and environmental samples had similar alpha diversity, while beta-diversity showed differences among families (i.e. a dam and her litter), and association indexes revealed a significant correlation between family members and between sample origin, suggesting a strong contribution of the maternal flora to the initial seeding of the canine neonatal gut and a strong individual dam imprint. Conclusion: This study showed that the meconium of vaginally delivered puppies has its own microbiota immediately after birth, and that it is shaped by the dam, which gives a specific imprint to her litter. [ABSTRACT FROM AUTHOR]

Introduction: While rabies remains a global concern, detailed studies on human rabies, particularly regarding causal animals and the reasons for not receiving postexposure prophylaxis (PEP), are lacking. Methods: We conducted a 3-year prospective study (October 2019–September 2022) at the Philippines’ largest rabies referral center. We interviewed patients with suspected rabies and their families. We used LN34 qRT-PCR and rapid fluorescent focus inhibition test on saliva samples. We also compared our findings with two retrospective studies at the same hospital. Results: We enrolled 151 patients, including 131 with potential rabies exposure. Similar to retrospective studies, the participants were predominantly males (75.5%), adults (76.8%), low-income individuals (91.4%), and rural dwellers (62.3%). The causal animals were mainly dogs (97.0%), with similar incubation periods, clinical symptoms, and a high proportion not receiving vaccines or immunoglobulins (93.2%). Most causal animals were owned by either the patients’ households or their neighbors (60.2%), with a significant proportion being puppies (58.8%). Most patients had knowledge of rabies; however, reasons for not seeking PEP included misconceptions about minor bites not causing rabies (51.3%), beliefs in traditional healers (33.9%), and economic constraints (22.6%). Despite completing the WHO regimen, two PEP failures were observed. LN34 qRT-PCR detected 98 positive cases (sensitivity, 64.9%; 95% CI 56.7–72.5). These strains belong to the Southeast Asia 4 subclade. Discussion: In conclusion, this study highlights the role of puppies as primary causal animals and the presence of misconceptions that preclude patients from acquiring PEP. [ABSTRACT FROM AUTHOR]

Capnocytophaga canimorsus and Capnocytophaga cynodegmi are commensal bacteria in the oral cavities of dogs. Both are zoonotic pathogens that could infect humans via dog bites. C. canimorsus may cause life-threatening infections in humans, whereas C. cynodegmi infections tend to be milder and more localized. Capsular serovars A–C of C. canimorsus seem to be virulence-associated. Some of the C. canimorsus serovars described to date can also be detected in other Capnocytophaga species, including C. cynodegmi. The objective of this pilot study was to investigate the emergence of C. canimorsus and C. cynodegmi after birth in oral cavities of puppies and to evaluate the impact of the dam's Capnocytophaga spp. carrier status on the emergence. Ten litters, altogether 59 puppies, were included in the study. The puppies and their dams were sampled at five time points over seven weeks after whelping. Oral swab samples taken were investigated for the presence of C. canimorsus and C. cynodegmi by species-specific polymerase chain reaction (PCR), the specificity of which was verified by sequencing a selection of the PCR products. Samples that were positive in Capnocytophaga PCR reactions were also capsular-typed by PCR to gain more knowledge about the Capnocytophaga spp. present in the samples. Altogether 10.2% and 11.9% of puppies, or 20.0% and 30.0% of litters tested PCR-positive for C. canimorsus and C. cynodegmi, respectively. Capnocytophaga PCR-positive puppy samples were always positive for only C. cynodegmi or C. canimorsus, not both. Most Capnocytophaga PCR-positive puppies became positive at the age of 5 to 7 weeks. Only a minority (5/16) of the C. cynodegmi PCR-positive dog samples were positive in capsular typing PCR, whereas all C. canimorsus PCR-positive dog samples were negative in capsular typing PCR. For all Capnocytophaga PCR-positive puppies, their dam was positive for the same Capnocytophaga species. These results suggest that puppies become colonized by C. cynodegmi or C. canimorsus from their dams at the time of deciduous teeth eruption. [ABSTRACT FROM AUTHOR]

Jesus' response to the Syrophoenician woman in Mark 7.27 is sometimes seen as sexist, racist, or abusive. The force of his response depends in part on the diminutive form κυναριον, which is often dismissed as a faded diminutive that lacks true force. But a statistical, semantic, and contextual analysis of the word indicates that it does, in fact, have diminutive force in Mark 7:27. Because of this, the pejorative force found in direct insults employing the word 'dog' is lacking in Jesus' response. In addition to failing to recognise the diminutive force of κυναριον, interpreters sometimes assume a social context in which Jews routinely referred to Gentiles as dogs. Finally, the analogy that Jesus makes is often read allegorically, assuming that 'children' and 'dogs' have direct counterparts in 'Jews' and 'Gentiles'. These assumptions are found to be dubious. The point of Jesus' analogy is about the proper order of events: children eat before the puppies; Jews receive the benefits of his ministry before Gentiles. The Syrophoenician woman outwits Jesus by arguing that the puppies may eat simultaneously with the children. The interpretive upshot is that Jesus' saying is unlikely to be misogynistic or abusive, but simply asserts Jewish priority, a priority that admits of exceptions and change. [ABSTRACT FROM AUTHOR]

Most canine gliomas occur in adult and aged dogs, and reports in puppies < 12-mo-old are exceedingly rare. Here we describe the occurrence of gliomas in 5 dogs ≤ 12-mo-old. The affected patients (4 males, 1 female) were 3–12-mo-old (x̄ = 6.6-mo-old). None of the dogs were brachycephalic. Clinical signs consisted of dullness (2 cases), seizures (2 cases), vestibular signs, and deafness (1 case each). All patients were euthanized. Grossly, neoplasms were pale-tan or red, soft masses in the telencephalon (4 cases) or gelatinous leptomeningeal thickening in the brain and spinal cord (1 case). Neoplasms were classified as astrocytomas (3 cases) and oligodendrogliomas (2 cases) based on histology or histology and IHC. Our findings confirm that, while exceptionally rare, canine gliomas occur in the first year of life, and are clinically, morphologically, and immunohistochemically similar to gliomas in adult and aged dogs. [ABSTRACT FROM AUTHOR]

Babesia gibsoni is a parasitic protozoan transmitted through tick bites and can cause severe disease in dogs. It can also be transmitted through direct contact with infected blood during dog fights, blood transfusions, and from dam to offspring during the perinatal period, resulting in stillborn or dead newborn puppies. This study aimed to determine the incidence of infection, the viability of newborn puppies, and the degree of B. gibsoni transmission from infected dam to offspring during pregnancy and lactation. Using PCR-based molecular methods, B. gibsoni infection in a pregnant American Pit Bull Terrier and her newborn puppies was confirmed. The incidence of B. gibsoni infection in the litter reached 75%. Out of eight puppies, six were infected with B. gibsoni, and one died. A therapeutic protocol comprising Malarone®, azithromycin, and artesunate was administered to a lactating B. gibsoni-positive bitch. By day 77 after birth, three out of five positive puppies showed negative PCR tests for B. gibsoni, indicating successful treatment through breast milk during nursing. In the two remaining positive puppies, therapy was started and parasitemia was successfully eliminated. [ABSTRACT FROM AUTHOR]

Abstract. The temperament of a total of 30 small puppies of 4 breeds in the period of their primary socialization was typified. The number of sanguines, cholerics, phlegmatics and melancholics was determined separately according to the breed of the animals along with the possibility of building a successful behavioral model for representatives of different breeds according to their temperament. The fastest socialization was reported in Shih Tzu puppies, followed by Bichon Frise, Pekingese, Dachshund, and lastly Poodle. There was a relationship between temperament and breed in dogs.

Simple Summary: Screening puppies carefully for heart murmurs during the first veterinary health visit is essential to disclose hidden congenital heart diseases. However, murmurs in puppies can also be innocent. Ideally, puppies with a possible congenital heart disease should not be sold to a new owner by their breeders. Our study aimed to investigate how easy veterinarians find differentiating innocent from pathologic murmurs, and how they manage puppies with a pathological murmur. We sent a questionnaire to veterinary practices in the Netherlands and Belgium, and analyzed 452 responses. Though 88% of the respondents find detecting a heart murmur easy, only 9% find differentiating innocent from pathologic murmurs in puppies easy. Only 80% of veterinarians recommend immediate referral of puppies with a pathologic heart murmur. Most respondents recognize that normal growth and lack of clinical signs do not rule out congenital heart disease. Though there are several centers with a veterinary cardiology specialist employed in the surveyed countries, only 43% of the respondents recommended the referral of a puppy to a veterinary cardiology specialist for murmur investigation. In conclusion, most veterinarians understand the importance of an echocardiography of puppies with loud murmurs, but they acknowledge the difficulty in distinguishing innocent from pathological murmurs. Background: Heart murmurs in puppies can be innocent or pathologic; the latter is almost always related to a congenital heart disease. Differentiating between these murmurs can be challenging for practicing veterinarians, but this differentiation is essential to ensure the best prognosis for puppies having a congenital heart disease. Our study aimed to reveal how veterinarians manage puppies with a heart murmur. Methods: A web-based questionnaire was sent to Dutch and Belgian veterinary practices. Results: Data from 452 respondents were analyzed. Though 88% of the respondents find detecting a heart murmur easy, only 9% find differentiating innocent murmurs from pathologic murmurs in puppies easy. Of the respondents, only 80% recommend immediate additional examination when detecting a loud heart murmur during the first veterinary health check at 6 weeks of age. Most of the respondents are aware that normal growth and the absence of clinical signs do not exclude severe congenital heart disease. Of the respondents, 31% were uncertain whether early surgical intervention could lead to improved outcomes. Conclusions: Veterinarians are aware of the importance of echocardiography for puppies with a loud heart murmur, and recognize their limitations when differentiating an innocent from a pathological heart murmur in a puppy. [ABSTRACT FROM AUTHOR]

Introduction: A healthy gastrointestinal (GI) microbiome has been shown to be essential for proper nutrient absorption and metabolism, maintenance of intestinal epithelial integrity and osmolarity, gut immunomodulation, and overall health. One of the most effective ways to promote a healthy GI microbiome is through dietary interventions, such as the addition of prebiotics. Prebiotics are substrates that are selectively utilized by the host GI microbiome through fermentation to confer a health benefit. However, research on prebiotics in companion animals is limited, especially in growing animals. Thus, this study was conducted to assess the effects of a novel prebiotic fiber blend on key parameters related to intestinal health and growth in puppies. Methods: Twenty-two puppies at least 4 months of age but not older than 10 months were fed a commercially available dry food during a prefeed period, and then fed a similarly formulated test food with the addition of the prebiotic fiber blend for a minimum of 90 days. Serum and fecal samples were collected at the end of the prefeed period and throughout the test period. Results: Puppies fed the test food grew as expected for puppies of this age. Complete blood count and serum chemistry analyses were clinically normal for all animals. Fecal score increased linearly, fecal moisture decreased linearly, and pH exhibited a cubic trend throughout the study duration. There was a linear increase in short-chain fatty acids throughout the study, which is associated with favorable digestive and overall health. The inflammatory cytokine interleukin-7 decreased linearly and interleukin-18 trended towards linear decrease. Conclusion: This study showed that puppies continued to grow and develop normally, and experienced serum and stool characteristics indicative of improved GI health when fed a growth food fortified with a novel prebiotic fiber blend. Furthermore, these results contribute to the overall understanding of the effects of prebiotics on the GI health of growing companion animals. [ABSTRACT FROM AUTHOR]

The temperament of a total of 30 small puppies of 4 breeds in the period of their primary socialization was typified. The number of sanguines, cholerics, phlegmatics and melancholics was determined separately according to the breed of the animals along with the possibility of building a successful behavioral model for representatives of different breeds according to their temperament. The fastest socialization was reported in Shih Tzu puppies, followed by Bichon Frise, Pekingese, Dachshund, and lastly Poodle. There was a relationship between temperament and breed in dogs. [ABSTRACT FROM AUTHOR]

Canine parvovirus 2 (CPV-2) is a highly contagious enteric virus that causes high morbidity and mortality, especially in dogs under six months of age. Recovery from this illness is dependent on several factors, including the patient's prognosis for adequate therapy. The aim of this study was to evaluate the risk factors associated with the death outcome in CPV-2 positive dogs in a case-control study conducted at the Veterinary Hospital of the Universidade Federal de Lavras (HV-UFLA) in Lavras, Minas Gerais, Brazil. Twenty-six dogs with CPV-2 symptoms that arrived at the HV-UFLA between 2017 and 2018 were evaluated for inclusion in the study. Data on medical history, clinical signs, blood count and rapid test of parvovirus and faecal test for polymerase chain reaction (PCR) were collected for all the animals. All the dogs received treatment at the HV-UFLA, and the overall fatality rate due to canine parvovirus was 30.77%. Descriptive analysis and univariate and multivariate statistical analyses (logistic regression) were performed to assess the variables that were possibly associated with an unfavourable prognosis (death). In the univariate and multivariate analyses, Systemic Inflammatory Response Syndrome (SIRS) was observed to be a significant risk factor for an unfavourable prognosis in canine parvovirus, as it increased the risk of death by 12.96 times (95% CI 1.85-133.70; P < 0.01) compared with patients who did not exhibit SIRS. Thus, SIRS was strongly associated with an unfavourable prognosis, suggesting that it can be used as a prognostic indicator for canine parvovirus in veterinary practice. [ABSTRACT FROM AUTHOR]

A 10‐week‐old Pembroke Welsh Corgi was presented for unproductive retching and abdominal distension to a referral hospital in Brisbane, Australia. The puppy had a history of polydipsia and was reported to have been drinking excessively prior to presentation. A 360° gastric dilatation and volvulus (GDV) was diagnosed radiographically. The puppy underwent surgery for gastric derotation and incisional gastropexy. Follow‐up testing was consistent with congenital renal dysplasia. To the author's knowledge, there are no previous case reports of a GDV in a medium‐breed puppy. Overindulgence of water has not previously been identified as a risk factor for GDV in the literature. [ABSTRACT FROM AUTHOR]

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In mammals, offspring vocalizations typically encode information about identity and body condition, allowing parents to limit alloparenting and adjust care. But how do these vocalizations mediate parental behavior in species faced with the problem of rearing not one, but multiple offspring, such as domestic dogs? Comprehensive acoustic analyses of 4,400 whines recorded from 220 Beagle puppies in 40 litters revealed litter and individual (within litter) differences in call acoustic structure. By then playing resynthesized whines to mothers, we showed that they provided more care to their litters, and were more likely to carry the emitting loudspeaker to the nest, in response to whine variants derived from their own puppies than from strangers. Importantly, care provisioning was attenuated by experimentally moving the fundamental frequency (fo, perceived as pitch) of their own puppies' whines outside their litter-specific range. Within most litters, we found a negative relationship between puppies' whine fo and body weight. Consistent with this, playbacks showed that maternal care was stronger in response to high-pitched whine variants simulating relatively small offspring within their own litter's range compared to lower-pitched variants simulating larger offspring. We thus show that maternal care in a litter-rearing species relies on a dual assessment of offspring identity and condition, largely based on level-specific inter- and intra-litter variation in offspring call fo. This dual encoding system highlights how, even in a long-domesticated species, vocalizations reflect selective pressures to meet species-specific needs. Comparative work should now investigate whether similar communication systems have convergently evolved in other litter-rearing species. [ABSTRACT FROM AUTHOR]

Simple Summary: For dogs and their owners to live in harmony, owner expectations and the behaviour of their dogs need to be aligned. Limited socialisation can contribute to the development of undesirable behaviours, so the reduced socialisation opportunities for many puppies during the COVID-19 lockdown is a concern. This United Kingdom/Republic of Ireland study used data collected between May 2016 and November 2022 to examine the impact of age and lockdown phase (pre-, during, and post-) on the types of socialisation experiences of 8-to-19-week-old puppies and the recency of socialisation experiences of 6-month-old puppies. The findings showed that puppies under 19-weeks had more types of experiences as they aged, and during pre-lockdown compared to post-lockdown, but not between other lockdown phases. Most 6-month-old puppies had met new adults or dogs, familiar dogs, or children within the last 1–7 days, regardless of lockdown phase. However, during lockdown, 6-month-old puppies experienced longer periods between meeting new adults in their homes. Overall, lockdown had a smaller impact on socialisation experiences than expected, but the quantity and quality of these experiences may have been affected. Future research will explore whether these early-life experiences relate to adult behaviour as the dogs in the study grow older. Limited socialisation can contribute to the development of undesirable dog behaviours. The COVID-19 lockdown potentially limited socialisation opportunities, which may negatively impact the future behaviour of puppies raised during lockdown. Data were gathered from longitudinal study participants in the United Kingdom/Republic of Ireland via multiple questionnaires between May 2016 and November 2022. The impact of age and lockdown phase (pre-, during, and post-) on the types of socialisation experiences of 8-to-19-week-old puppies and the recency of socialisation experiences of approximately 6-month-old puppies were examined. Puppies under 19-weeks had significantly more types of socialisation experiences (from a predefined list) as they aged, and pre-lockdown compared to post-lockdown, but not between other lockdown phases. Most 6-month-old puppies had met a new adult or dog outside the household, a familiar dog, and/or a child within the last 1–7 days, and this was similar between lockdown phases. During lockdown, 6-month-old puppies experienced longer periods between meeting a new adult in their home. Puppies were hypothesised to have had fewer experiences during lockdown, but this was not found. However, the quantity and quality of these experiences may have been affected. Future research within this longitudinal study will explore relationships between the timing and type of experiences had by puppies and their subsequent behaviour. [ABSTRACT FROM AUTHOR]

Objective: To assess whether gonioscopy performed on Samoyed puppies (6–14 weeks of age) is a reliable predictor of their gonioscopy results as adults (>11 months of age). Procedure: A retrospective medical record search was performed to identify Samoyed dogs that had undergone gonioscopy as a puppy (6–14 weeks of age). A second prospective gonioscopy examination was performed as an adult (>11 months of age) and the results were compared. Gonioscopy was assessed on two characteristics: percent of iridocorneal angle (ICA) unaffected by pectinate ligament abnormalities (PLA) and ICA width, with PLA results categorized into unaffected (≥75% open), moderately affected (50%–74% open), and severely affected (<50% open) and the width categorized into wide, moderate, narrow, and closed groups. A multivariate model was created that considered factors such as PLA, angle width, sex, puppy age, neuter status, and time between examinations. Results: The eyes of 77 Samoyed dogs (154 eyes) met inclusion criteria. When assessing PLA, 90% of dogs had the same categorization as a puppy and as an adult. When assessing ICA width, 53.2% of dogs had the same width classification as a puppy and as an adult. There is strong evidence that variation between puppy and adult results increases with time between examination (p =.03). Conclusion: Puppies unaffected by PLA and with a wide ICA angle, are likely to retain these features as adults. Selection of puppies with desirable gonioscopic traits may help breeders to choose dogs likely to retain these traits as adults for breeding. This may reduce the incidence of glaucoma in the breed. Puppy examinations should be performed in both eyes. Examination of adults prior to breeding remains prudent. [ABSTRACT FROM AUTHOR]

Two 9‐week‐old female littermate German Shepherd puppies showed severe high‐frequency low‐amplitude trembling that worsened with movement. The white matter (WM) of the central nervous system (CNS) showed bilateral diffuse severe spongiosis in the cerebellum, brainstem, spinal cord, and the neuropil of the oculomotor and red nuclei. The cortical corona radiata was less severely affected. Rare necrotic or apoptotic glia‐like cells also were identified in the WM. Luxol fast blue staining disclosed severe diffuse myelin loss in the entire CNS; peripheral nerves were spared. Glial fibrillary acidic protein immunohistochemistry showed diffuse astrogliosis and astrocytosis in the WM. Genetic analyses of the littermates excluded the aspartoacylase (ASPA) gene as a candidate for this condition in dogs. In conclusion, this description of a rare congenital spongiform leukodystrophy in the German Shepherd breed, closely resembling to Canavan disease in humans, is likely caused by a genetic alteration unrelated to the ASPA gene. [ABSTRACT FROM AUTHOR]

Families commonly document their outings by capturing their experiences through digital photographs and videos. However, little is known about the ways in which families engage their personal mobile devices to document educational family outings and how they subsequently talk about the digital artifacts that captured their informal learning experiences. This paper presents new evidence on family digital artifact creation during an informal science institution (ISI) visit, the expected and actual uses of their digital artifacts after the visit, and family conversations reminiscing about the ISI visit with the digital artifacts. Using a concurrent triangulation mixed‐methods design, data on family digital artifact creation during an aquarium visit (N = 204) and digital artifact use after the visit (n = 67) were collected using parent surveys. Audio‐recorded parent‐child conversations with a subset of families (n = 25) document whether and how families use their digital artifacts to reminisce about the aquarium visit. Quantitative findings detail family digital technology practices during informal learning experiences, and qualitative findings suggest evidence of informal science learning in the everyday interactions of the families who elected to continue the study. The study indicates that family storytelling and digital technology practices may help to bridge informal science learning from ISI to home and frames family non‐participation within an anti‐deficit perspective. [ABSTRACT FROM AUTHOR]

The emergence of canine parvovirus (CPV) in 1978, probably as a result of the cross-species incursion of feline panleukopenia virus, resulted in the current pandemic of canine parvoviral enteritis. It has been 40 years since the virus was first identified in Nigeria and it has been afflicting dog population in the country unabatedly. As such, in this review, CPV molecular epidemiology in Nigeria entailing its prevalence, occurrence of subtypes, co-infection and genetic evolution are analysed. All the three subtypes of the virus have been identified in the country with CPV-2a subtype being preponderant. However, in recent years there has been an upsurge in the number of CPV-2c and it is often associated with bloody diarrhoea even in vaccinated puppies. Therefore, there is need for proper assessment of the molecular epidemiology of the virus for proper institution of effective control policies to eradicate this pathogen. [ABSTRACT FROM AUTHOR]

Background and Aim: The Apgar score is a useful assessment of neonatal viability in dogs. The Apgar score in puppies born by cesarean section can be lower than vaginal delivery because all anesthetic drugs can cross the placenta. Therefore, anesthetic drugs with minimal cardiorespiratory effect and rapid elimination are recommended for cesarean section. The present study aimed to compare Apgar scores in puppies born after the induction of etomidate, alfaxalone or propofol, and those maintained with isoflurane inhalation during cesarean section. Materials and Methods: Thirty-six bitches were equally divided in the three anesthetic drug groups. Modified Apgar scores were assessed at 5, 15, and 60 min after delivery. Intraoperative vital signs and Apgar scores were compared using a linear mixed model and adjusted pairwise comparisons using Bonferroni analysis. Results: A total of 125 puppies were included in this study. Age, body weight, litter size, type of surgery, delivery time, anesthetic and surgical duration, and intraoperative vital signs did not significantly differ between the groups. Puppies in the alfaxalone and propofol groups had significantly higher Apgar scores than the etomidate group in both elective and emergency surgery. In elective surgery, Apgar scores at 5 min after delivery did not differ significantly between groups. At 15 and 60 min after delivery, Apgar scores in the etomidate group were significantly lower than those in the other groups. In emergency surgery, Apgar scores were significantly lower in the etomidate group than in the alfaxalone group at all time points. Conclusion: Induction with alfaxalone and propofol resulted in better outcomes with higher Apgar scores and neonatal viability than etomidate. Therefore, alfaxalone and propofol should be used as anesthetic induction drugs in both elective and emergency cesarean sections.

ABSTRACT Canine parvovirus 2 (CPV-2) is a contagious high-risk virus in dogs, which emerged as an important pathogen in 1978. There are limited investigations that explore maternally derived antibody (MDA) in canine parvovirus in puppies around the world. Furthermore, there is no such research in any province of Iran. This study measured the serum level of MDA against parvovirus in 42 puppies (21 puppies with vaccinated bitches and 21 puppies with unvaccinated bitches) and the serum level of canine parvovirus antibodies of their bitches (n=28) (21 vaccinated and 7 unvaccinated bitches). Antibodies against parvovirus were measured using quantitative, enzyme-linked immunosorbent assay (ELISA). Our results showed that 62% (13 out of 21) of puppies from vaccinated bitches and 76% (16 out of 21) of puppies from unvaccinated bitches were positive for anti-parvovirus antibodies, which wasn’t significantly different (P=0.253). Moreover, puppies’ titers weren’t statistically different in vaccinated and unvaccinated groups (P=0.476). There was a similar condition between vaccinated and non-vaccinated bitches (P=0.583). There was no relationship between breed and sexuality with vaccination status (Ps>0.05).

To determine the etiological agents responsible for acute pneumonia in puppies in China, this study utilized bronchoalveolar lavage (BAL) fluid extraction to enable the isolation, culture, biochemical identification, and 16S rRNA PCR amplification of the pathogens. Following preliminary identification, the pathogens underwent analysis for antibiotic resistance phenotypes and resistance genes. Additionally, the study examined the presence of virulence genes, conducted multilocus sequence typing (MLST), and performed whole-genome sequencing (WGS). The findings revealed that all four isolated pathogens were characterized as extraintestinal pathogenic Escherichia coli (ExPEC). The examined ExPEC strains demonstrated resistance to cephalosporins, tetracyclines, and penicillins, while remaining susceptible to aminoglycosides, beta-lactamase inhibitors, carbapenems, chloramphenicols, and sulfonamides. An analysis of virulence genes identified the presence of eight genes, namely CNF-I, fyuA, fimC, papC, ompA, fimH, irp2, and iroN, which are implicated in their invasiveness and potential to inflict tissue damage. The MLST analysis revealed that all ExPEC strains were classified under either sequence type ST131 (Achtman database) or ST43 (Pasteur database). The study further determined that these strains were absent in the kennel’s drinking water source, thereby ruling out water contamination as a potential factor in the emergence of ST131-type ExPEC. This study offers a theoretical framework and empirical evidence for elucidating the potential pathogenic mechanisms and clinical therapeutic strategies of ExPEC in the etiology of acute pneumonia in puppies.

Background and Aim: The Apgar score is a useful assessment of neonatal viability in dogs. The Apgar score in puppies born by cesarean section can be lower than vaginal delivery because all anesthetic drugs can cross the placenta. Therefore, anesthetic drugs with minimal cardiorespiratory effect and rapid elimination are recommended for cesarean section. The present study aimed to compare Apgar scores in puppies born after the induction of etomidate, alfaxalone or propofol, and those maintained with isoflurane inhalation during cesarean section. Materials and Methods: Thirty-six bitches were equally divided in the three anesthetic drug groups. Modified Apgar scores were assessed at 5, 15, and 60 min after delivery. Intraoperative vital signs and Apgar scores were compared using a linear mixed model and adjusted pairwise comparisons using Bonferroni analysis. Results: A total of 125 puppies were included in this study. Age, body weight, litter size, type of surgery, delivery time, anesthetic and surgical duration, and intraoperative vital signs did not significantly differ between the groups. Puppies in the alfaxalone and propofol groups had significantly higher Apgar scores than the etomidate group in both elective and emergency surgery. In elective surgery, Apgar scores at 5 min after delivery did not differ significantly between groups. At 15 and 60 min after delivery, Apgar scores in the etomidate group were significantly lower than those in the other groups. In emergency surgery, Apgar scores were significantly lower in the etomidate group than in the alfaxalone group at all time points. Conclusion: Induction with alfaxalone and propofol resulted in better outcomes with higher Apgar scores and neonatal viability than etomidate. Therefore, alfaxalone and propofol should be used as anesthetic induction drugs in both elective and emergency cesarean sections. [ABSTRACT FROM AUTHOR]

Social support can play a crucial role in enabling individuals to cope better with environmental stressors when accompanied by a social partner, but this effect varies strongly across species and often with partner identity. While generally conspecifics can provide social support most effectively, domesticated species might benefit also from the presence of a human partner; even more than their intensively human-socialized wild relatives. Here we set out to compare stress support provided by conspecific and human partners in equally hand-raised and kept wolf, Canis lupus , and dog, Canis lupus familiaris , puppies. Given their similar developmental trajectories and evolutionary background, we expected a similar supportive role of conspecifics in both species. Moreover, we hypothesized that domestication has prepared dogs to benefit from the presence of a human more than wolves, which, however, may be masked when intensive socialization is provided to both species. Therefore, we expected a similar stress buffering effect of a human partner in both dog and wolf puppies. To test this, we had puppies of both species participate in two neophobia tasks and one isolation-reunion-separation task. Although we found no direct differences between the two species when tested with either a conspecific or a human partner, there were a few behavioural differences between wolf and dog puppies suggesting that dogs rely more on humans than wolves. Our present results provide evidence that stress buffering has not evolved anew during the process of domestication, but also that both socialization and domestication might play a role in shaping the extent to which young dogs and wolves rely on their human partners. • We analysed social stress buffering in intensively socialized dog and wolf puppies. • Both conspecifics and humans provided similar social support to both species. • Dog puppies seem to rely on the human presence more than wolves. • When intensively socialized, also wolves benefit from the presence of a human. • Domestication did not affect the ability to benefit from the presence of a partner. [ABSTRACT FROM AUTHOR]

A 2‐year‐old Pomeranian‐Spitz dog (5.8 kg) was admitted with symptoms of uterine prolapse and lethargy 1 day after whelping three puppies. The prolapsed uterus was corrected, but the next day, prolapse reoccurred. To fix the cervix to the abdominal wall, the dog underwent a surgery operation where intussusception in the left horn of the uterus was found. Ovariohysterectomy was performed as the treatment of choice. The diagnosis of intussusception is challenging, and exploratory laparotomy is the reliable diagnostic approach. Although uterine intussusception is rare, it should be one of the important considerations in dogs within the post‐partum period. [ABSTRACT FROM AUTHOR]

Pet food manufacturers aim to balance the palatability with the nutritional value of their products. Ensuring that young canines receive the best nourishment is crucial, while also promoting long-lasting engagement and satisfaction during mealtime. The study aimed to investigate if the nutritional content of food can affect food preferences in 2-month-old Sharplanina shepherd dogs and the development of neophobia when introduced to a new diet. Three different dietary plans were tested, each with varying percentages of animal-derived proteins (97% in A, 77% in B, and 94% in C). Observations of behavior were documented on camera at the beginning and end of a 10-day feeding cycle. The observations were made during the scheduled feeding times of 7 a.m., noon, and 5 p.m. According to the study, puppies showed neophobic behavior when a new diet was introduced. The puppies displayed a notable decrease in meal rate of consumption, heightened distraction during diet consumption, and increased hesitation on the first day of each new diet, specifically on day 9 and day 10. Post-consumption interest peaked significantly on day 9 and day 10, particularly when dogs consumed diet C. Through the study, it was observed that Diet C had an impact on the puppies' feed preferences, indicating a possible link between the diet's nutritional content and their food preferences. Based on the study results, it appears that puppies need at least 9 days to reduce neophobia and adapt to new food flavors and feeding schedules. • Single pan test was used to examine feeding behavior in puppies. • The puppies experienced neophobia at the beginning of observation period. • Adapting to new food takes at least 9 days. • Diet with 94% animal derived proteins increased puppies' feed preferences. [ABSTRACT FROM AUTHOR]

A 4‐year‐old, female, entire, miniature, smooth‐haired dachshund was admitted for investigation of acute onset of spinal hyperalgesia and paraplegia. Ten days before presentation, the dog had undergone a caesarean section. Five dead/necrotic puppies were removed. A diagnosis of an aortic thrombosis was reached. Medical management resulted in a significant improvement of the neurological function. The aortic thrombosis was suspected to be secondary to the pregnancy and Caesarean section. [ABSTRACT FROM AUTHOR]

Simple Summary: When dealing with fragile subjects such as newborns, veterinarians can face clinical challenges. Data on the safety and efficacy of many medications used in puppies are surprisingly scarce. As a result, puppies are sometimes given ineffective medications or medications with unknown harmful side effects. This has prompted many professionals, and even more dog owners, to turn to the use of herbal remedies because of their easy access, convenience, greater tolerability, and wide margin of safety, thus leading them to becoming more popular worldwide. However, natural = safe is not always the case. The purpose of this review is to illustrate, based on the limited bibliography available, the areas of application of phytotherapeutic drugs in canine pediatrics while also pointing out the limitations of this therapeutic practice. Phytotherapy is a clinical modality that incorporates botanical remedies as part of the therapeutic approach. It is a very ancient branch of medicine that is currently undergoing a renaissance, evident in the numerous preparations available on the market. The majority of these formulations are for preventive and curative use in adult animals. Experimental experiences in the pediatric age group are particularly scarce within the existing literature. Since these products are readily accessible, dog owners often turn to them due to their ease of availability, a preference for self-medication, and the perception that herbs are safer, gentler, and less expensive than conventional medications, often leading them to bypass seeking the advice of experienced professionals. The purpose of this review is to illustrate, on the basis of the currently available bibliography, phytotherapeutic approaches in canine pediatrics, paying particular attention to the adverse effects resulting from the use of certain plants, even when used in conjunction with some synthetic drugs. Consequently, it becomes evident that further clinical and more relevant studies, specifically focusing on puppies, are needed to increase knowledge about the effects of herbal remedies. [ABSTRACT FROM AUTHOR]

A 7‐month‐old intact female bearded collie dog was admitted after a 2‐week history of progressive cough, inappetence, and lethargy, with no response to previous treatment with doxycycline and steroids. Mild attenuation of lung sounds in the right middle hemithorax was the only abnormality detected on physical examination. Abdominal ultrasound and thoracic radiographs were performed and revealed multifocally distributed nodules and masses, well‐circumscribed and of variable size in the kidneys and pulmonary parenchyma. Ultrasound‐guided fine needle aspirates of the renal and pulmonary masses were taken. A cytologic evaluation of these lesions pointed towards a malignant mesenchymal neoplasia. Euthanasia was elected due to the poor prognosis and rapid progression. The post‐mortem histopathology, a positive result to IBA1 immunoperoxidase staining, and a lack of detection of infectious agents, and negative E‐cadherin immunostaining enabled the final diagnosis of a disseminated histiocytic sarcoma. We report an atypical form, both in breed and age, of canine disseminated histiocytic sarcoma. While all breeds can be affected, there is a clear predisposition in some, and no cases have been previously described in bearded collies. Moreover, to the authors' knowledge, this is the youngest dog with this histiocytic disorder described to date. Disseminated histiocytic sarcoma should be considered as a differential diagnosis of multinodular tumors in dogs, regardless of the anatomic location and age of the dogs, even in puppies. [ABSTRACT FROM AUTHOR]

Canine herpesvirus 1 (CaHV-1) infects dogs, causing neonatal death and ocular, neurological, respiratory, and reproductive problems in adults. Although CaHV-1 is widespread in canine populations, only four studies have focused on the CaHV-1 whole genome. In such context, two CaHV-1 strains from both the kidney and spleen of 20-day-old deceased French Bulldog puppies were recently isolated in Sardinia, Italy. The extracted viral DNA underwent whole-genome sequencing using the Illumina MiSeq platform. The Italian CaHV-1 genomes were nearly identical (>99%), shared the same tree branch, and clustered near the ELAL-1 (MW353125) and BTU-1 (KX828242) strains, enlarging the completely separated clade discussed by Lewin et al., in 2020. This study aims to provide new insights on the evolution of the CaHV-1, based on high-resolution whole-genome phylogenetic analysis, and on its clinicopathological characterization during a fatal outbreak in puppies. [ABSTRACT FROM AUTHOR]

Simple Summary: Life inside the uterus is possible only thanks to the placenta, an essential structure that undergoes modifications to support the growth and development of the fetus. For this reason, the placenta is also described providing as a "diary of intrauterine life" and is a potential source of information regarding events that occur during gestation. Few studies, however, have considered the evaluation of this organ in the management of canine neonates. In the present study, the efficiency of the placenta was evaluated in 69 live puppies delivered by 15 large-breed bitches. The data reported in the present study show that in a subgroup of puppies, despite a normal placental weight and structure, reduced placental efficiency was identified. Although the findings should be confirmed in a larger population, the significantly increased risk of death in low-efficiency placental puppies reported in this study suggest the usefulness of this parameter in the evaluation of the puppy in the first 7 days after delivery. Background: The anatomical and functional characteristics of the placenta influence the adaptive ability of the fetus to the extrauterine environment. Placental efficiency, measured as the gram of neonate produced by each gram of placenta, summarizes these characteristics. In the present study, placental efficiency and its impact on the 7-day postpartum life of the puppies were studied in canine large breeds. Methods: Placental efficiency (PE) was computed using chorioallantois weight (WPE) and surface (SPE) efficiency for puppies born from natural delivery or elective cesarean section. Capillary density was also histologically determined. Neonate viability was estimated by the APGAR score and the daily weight gain (DWG) was recorded on day 7 after delivery. Results: from 15 large-breed bitches, 69 live puppies were born by natural delivery (24 puppies) and elective cesarean section (45 puppies). Cluster analysis detected a group of neonates for which reduced placental efficiency (8 for the WPE, 9 for the SPE) was identified, despite a placental weight and surface within the mean and no difference in capillary density. In this group, the DWG was lower and the mortality within 7 days was higher. Conclusions: the results suggest that placental efficiency could be an additional tool for the evaluation of a puppy in the first 7 days after delivery. [ABSTRACT FROM AUTHOR]