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Background: Establishment of daycare blood transfusion service in a licensed blood transfusion center of Dhaka city for patients needed blood transfusion is a milestone for improving blood service of Bangladesh. Aim: The aim of this study is to assess the daycare blood transfusion service of a licensed blood transfusion center in Dhaka city and evaluation of patients receiving blood transfusion and to determine the pattern of blood groups of the patients. Materials and Methods: This cross-sectional study was done in a licensed blood transfusion center in Dhaka city. The study reveals that 383 patients of various modalities of ailments had their blood transfusion from August 2012 to January 2014. The patient age, sex, diagnosis, and levels of hemoglobin were taken into consideration. The patterns of their blood groups were determined for the study. Results: This cross-sectional study revealed that among 383 patients, 148 were male and 235 were female. With the age range of 14–80 years, mean age was 53.9 ± 2.77 years. Among them, most (148) were in the age range of 55–64 years. Among the patients, 47.00% (180) were suffering from anemia according to severe, moderate, and mild anemia with various diseases. Out of 383 patients, 31.33% (120) were suffering from carcinomas. Among those carcinomas, breast, colon, lung, larynx, tongue, stomach, and esophagus carcinoma and cholangiocarcinoma were prevalent. Others such as chronic kidney disease and leukemia 7.31% (28) were next to take transfusion. Maximum of them, i.e. 235 (61.36%), were in the hemoglobin range of 5.81–7.80 g/dl range and 60 (15.67%) had their hemoglobin level at 3.81–5.80 g/dl range. Out of them, 160 were of A group, 106 were B group, 88 were O group, and 29 were AB group. Conclusion: Although daycare transfusion centers are very few in licensed blood transfusion centers, the need for daycare transfusion is increasing gradually. We recommend establishing more daycare transfusion services for the transfusion needed patients.