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The research was conducted to complete technological protocol of biomass production of algae Nannochloropsis oculata with both culture methods in plastic bags and photo-bioreactor systems. The results show that in the nylon bag system, algae reached a peak of biomass density at day-8 of culture with 80 million cells per ml. In the photo-bioreactor systems, algae were higher density (180 million cells per ml), and longer period of culture (15 days of culture) than in plastic bags. The total products of 404.4 kg of fresh concetrated algae and 57.6 kg of dried algae powder which were suitable produced under climate conditions in Nghe An. All the algae products met the quality and requirements for use as food for aquatic animals as well as raw materials for the production of functional foods for humans.