753 results on '"plaagresistentie"'
Search Results
2. Bijzondere tuinen : De mooiste groenprojecten op een rij
- Published
- 2020
3. Resistentie tegen Phytophthora : voorkom doorbraak
- Abstract
Nieuwe Phytophthora resistente aardappelrassen vormen de inzet om biologische aardappelteelt zonder toepassing van gewasbescherming rendabel te maken voor de biologische telers. Het aanbod resistente aardappelrassen groeit gestaag. Veredelaars maken gebruik van verschillende resistentie bronnen tegen Phytophthora, maar het aantal resistentiegenen is beperkt dus daar moeten we heel zuinig op zijn. Een resistentie-doorbraak ligt altijd op de loer. Resistentie-management verkleint dit risico en is daarom noodzakelijk. Hoe is een doorbraak te voorkomen en wat vragen de nieuwe rassen aan maatregelen?
- Published
- 2018
4. Stadsnatuur en natuurinclusief plaagdierbeheer
- Abstract
Natuurinclusief ontwerpen en bouwen binnen de stad is trendy geworden. Het recente inspiratieboek Stadsnatuur Maken is een illustratie van die trend. Stadsnatuur is een rijke natuur, in tegenstelling tot onze agrarische buitengebieden die in toenemende mate worden bepaald door monoculturen van slechts een paar soorten planten. Sloten worden geschoond. Bermen worden geklepeld in plaats van gefaseerd maaien en afvoeren. Populaties van schadelijke diersoorten worden met biociden geminimaliseerd, dikwijls preventief. De biodiversiteit van onze buitengebieden is relatief arm geworden. Dat is de prijs die wordt betaald voor agrarische succesverhalen. De toepassing van gewasbeschermingsmiddelen draagt positief bij aan landbouwefficiency, maar de verarmde biodiversiteit is de andere kant van de medaille. Het buitengebied verwordt tot zombienatuur: het lijkt groen maar is zo dood als een pier. Natuurinclusieve steden bieden tegenwicht.
- Published
- 2017
5. Teeltrecepten voor nieuwe rassen : de strijd tegen phytophthora gaat altijd door, nieuwe rassen vragen extra aandacht van de telers
- Abstract
Nieuwe resistentie aardappelrassen moeten biologische aardappelteelt zonder toepassing van gewasbescherming rendabel maken voor de biologische telers. Het telen van deze resistente rassen vraagt aandacht en zorg. Zonder maatregelen in de vorm van resistentiemanagement ligt het risico van een ziektedoorbraak om de hoek. Welke teeltmaatregelen vragen de nieuwe rassen?
- Published
- 2017
6. Rassenproef aardappelen biologische teelt 2017
- Abstract
Droogte overheerste de rassenproef bio aardappel 2017. In het aanbod zien we het aandeel plaagresistente rassen sterk toenemen met ook nieuwe opties in het vastkokende segment.
- Published
- 2017
7. Host-plant resistance to western flower thrips in Arabidopsis
- Author
-
Manus P. M. Thoen, Wageningen University, Marcel Dicke, Harro Bouwmeester, and Maarten Jongsma
- Subjects
genomica ,data analysis ,Population ,optical tracking ,insect plant relations ,Biology ,Quantitative trait locus ,Plant disease resistance ,waardplanten ,Botany ,genomics ,plaagresistentie ,Plant defense against herbivory ,Laboratorium voor Plantenfysiologie ,Laboratory of Entomology ,education ,genoomanalyse ,genome analysis ,host-seeking behaviour ,education.field_of_study ,gedrag bij zoeken van een gastheer ,insect pests ,Thrips ,Abiotic stress ,defence mechanisms ,arabidopsis thaliana ,fungi ,food and beverages ,Laboratorium voor Entomologie ,insectenplagen ,biology.organism_classification ,pest resistance ,gegevensanalyse ,Western flower thrips ,insect-plant relaties ,host plants ,BIOS Applied Metabolic Systems ,PEST analysis ,EPS ,optisch sporen ,Laboratory of Plant Physiology ,verdedigingsmechanismen ,frankliniella occidentalis - Abstract
Western flower thrips is a pest on a large variety of vegetable, fruit and ornamental crops. The damage these minute slender insects cause in agriculture through feeding and the transmission of tospoviruses requires a sustainable solution. Host-plant resistance is a cornerstone of Integrated Pest Management (IPM). Plants have many natural defense compounds and morphological features that aid in the protection against herbivorous insects. However, the molecular and physiological aspects that control host-plant resistance to thrips are largely unknown. A novel and powerful tool to study host-plant resistance to insects in natural populations is genome-wide association (GWA) mapping. GWA mapping provides a comprehensive untargeted approach to explore the whole array of plant defense mechanisms. The development of high-throughput phenotyping (HTP) systems is a necessity when large plant panels need to be screened for host-plant resistance to insects. An automated video-tracking platform that could screen large plant panels for host-plant resistance to thrips, and dissect host-plant resistance to thrips in component traits related to thrips behavior, was developed. This phenotyping platform allows the screening for host-plant resistance against thrips in a parallel two-choice setup using EthoVision tracking software. The platform was used to establish host-plant preference of thrips with a large plant population of 345 wild Arabidopsis accessions (the Arabidopsis HapMap population) and the method was optimized with two extreme accessions from this population that differed in resistance to thrips. This method can be a reliable and effective high throughput phenotyping tool to assess host-plant resistance to thrips in large plant populations. EthoAnalysis, a novel software package was developed to improve the analyses of insect behavior. There were several benefits from using EthoAnalysis to analyze EthoVision data. The detailed event statistics that could be extracted from EthoAnalysis allows researchers to distinguish detailed differences in moving and feeding behavior of thrips. The potential of this additional information is discussed in the light of quantitative genetic studies. Stress resistance was studied in the HapMap population on a total of 15 different biotic and abiotic stresses ranging from biotic stresses like insects and nematodes, to abiotic stresses like drought and salt. A multi-trait GWA study to unravel the genetic architecture underlying plant responses to the different stresses was performed. A genetic network in this study revealed little correlation between the plant responses to the different insect herbivores studied (aphids, whiteflies, thrips and caterpillars). For thrips resistance a weak positive correlation with resistance to drought stress and Botrytis, and a negative correlation with resistance to parasitic plants were observed. One of the surprising outcomes of this study was the absence of shared major QTLs for host-plant resistance and abiotic stress tolerance mechanisms. RESISTANCE METHYLATED GENE 1 (RMG1) was one of the candidate genes in this multi-trait GWA study that could be controlling shared resistance mechanisms against many different stresses in Arabidopsis. RMG1 is a nucleotide-binding site Leucine-rich repeat (NB-LRR) disease resistance protein and its potential relation to several resistance/tolerance traits was successfully demonstrated with T-DNA insertion lines. The 15 stresses were used in a comparison with a metabolomics dataset on this Arabidopsis HapMap population. It was discovered that levels of certain aliphatic glucosinolates correlated positively with the levels of resistance to thrips. This correlation was further investigated with the screening of a RIL (Recombinant Inbred Line) population for resistance to thrips, several knockout mutants and the analysis of co-localization of GWA mapping results between glucosinolates genes and thrips resistance. In a GWA analysis, the C4 alkenyl glucosinolates that correlated the strongest with thrips resistance mapped to the genomic regions containing genes known to regulate the biosynthesis of these compounds. However, thrips resistance did not co-localize with any of the GSL genes, unless a correction for population stratification was omitted. Additional screening of a Cvi x Ler RIL population showed a QTL for thrips resistance on chromosome 2, but no co-localization with any known glucosinolate genes, nor with thrips resistance loci identified by GWA mapping. Knock-out mutants and overexpressors of glucosinolate synthesis genes could also not confirm a causal link between glucosinolates and resistance to thrips. It is possible that the crucial factors that control resistance to thrips may not have been present in sufficient quantities or in the right combinations in the mutants, RILs and NIL screened in this study. Alternatively, the correlation between thrips feeding damage and glucosinolate profiles could be based on independent geographical clines. More research should be conducted to assess which of these explanations is correct. In the general discussion, the results from this thesis are discussed in a broader perspective. Some prototypes of new phenotyping platforms that could further aid screening for resistance to thrips in the future are presented. Natural variation in host-plant resistance to thrips is compared to the variation in host-plant resistance to aphids and caterpillars. The geographic distribution of host-plant resistance to thrips is not evident in the other insects, in line with the distribution of glucosinolate profiles and other climate factors. The chapter concludes with some suggestions for future research in the field of host-plant resistance to thrips.
- Published
- 2016
8. Mapping moves on Arabidopsis : from natural variation to single genes affecting aphid behaviour
- Subjects
aphidoidea ,insect pests ,arabidopsis thaliana ,insect plant relations ,quantitative traits ,Laboratorium voor Entomologie ,functionele genomica ,genexpressie ,insectenplagen ,pest resistance ,insect-plant relaties ,feeding behaviour ,voedingsgedrag ,016-3933 ,BIOS Applied Metabolic Systems ,gene expression ,plaagresistentie ,genetic mapping ,Laboratorium voor Plantenfysiologie ,genetische kartering ,EPS ,Laboratory of Entomology ,functional genomics ,kwantitatieve kenmerken ,Laboratory of Plant Physiology - Published
- 2016
9. Unraveling molecular mechanisms underlying plant defense in response to dual insect attack : studying density-dependent effects
- Author
-
A. Kroes, Wageningen University, Marcel Dicke, and Joop van Loon
- Subjects
media_common.quotation_subject ,Insect ,insect plant relations ,Parasitoid ,moleculaire plantenziektekunde ,chemistry.chemical_compound ,Botany ,Plant defense against herbivory ,plaagresistentie ,molecular plant pathology ,Laboratory of Entomology ,media_common ,2. Zero hunger ,Herbivore ,Aphid ,density ,biology ,insect pests ,Diaeretiella rapae ,herbivory ,Jasmonic acid ,defence mechanisms ,fungi ,arabidopsis thaliana ,dichtheid ,food and beverages ,15. Life on land ,biology.organism_classification ,Laboratorium voor Entomologie ,insectenplagen ,pest resistance ,insect-plant relaties ,Brevicoryne brassicae ,chemistry ,016-3953 ,EPS ,herbivorie ,verdedigingsmechanismen - Abstract
In the field, plants suffer from attack by herbivorous insects. Plants have numerous adaptations to defend against herbivory. Not only do these defense responses reduce performance of the feeding herbivore, they also result in the attraction of natural enemies of herbivores. The majority of studies investigating plant-insect interactions addressed mainly the effects of attack by a single herbivore species on induced plant defenses. However, because plants are members of complex communities, plants are exposed to different insect attackers at the same time. Moreover, attacks by different herbivores interact at different levels of biological organization, ranging from the level of gene expression, phytohormone production and biochemical changes up to the individual level. Effects of plant responses to feeding by two or more herbivore species simultaneously might cascade through the community and thereby affect insect community composition. The induction of plant defense responses is regulated by a network of signaling pathways that mainly involve the phytohormones jasmonic acid (JA), salicylic acid (SA) and ethylene (ET). The signaling pathways of the two phytohormones SA and JA interact antagonistically, whereas JA and ET signaling pathways can interact both synergistically and antagonistically in regulating plant defense responses. In general, JA-mediated signaling underlies defense responses against leaf-chewing herbivores, such as caterpillars, whereas phloem-feeding insects, such as aphids, mainly induce SA-regulated defenses. When caterpillars and aphids simultaneously feed on the same host plant, crosstalk between phytohormonal signaling pathways may affect the regulation of plant defenses. Consequently, multiple insect herbivores feeding on plants interact indirectly through plant-mediated effects. Studies investigating molecular mechanisms underlying interference by multiple attacking insects with induced plant defenses will benefit studies on the ecological consequences of induced plant responses. The aim of this thesis was to elucidate molecular mechanisms that underlie plant-mediated interactions between attacking herbivores from different feeding guilds, namely Brevicoryne brassicae aphids and Plutella xylostella caterpillars. Because herbivore density affects the regulation of plant defense responses, it may also influence the outcome of multiple insect-plant interactions. To study if modulation of induced plant defenses in response to dual insect attack depends on insect density, plants were infested with two densities of aphids. Responses of Arabidopsis thaliana plants to simultaneous feeding by aphids and caterpillars were investigated by combining analyses of phytohormone levels, defense gene expression, volatile emission, insect performance and behavioral responses of parasitoids. To better predict consequences of interactions between plants and multiple insect attackers for herbivore communities, the regulation of defense responses against aphids and caterpillars was also studied in the ecological model plant wild Brassica oleracea. Transcriptomic changes of plants during multiple insect attack and their consequences for the plant’s interactions with members of the associated insect community take place at different time scales. Direct correlation of transcriptomic responses with community development is, therefore, challenging. However, detailed knowledge of subcellular mechanisms can provide tools to address this challenge. One of the objectives of this thesis, therefore, was to investigate the involvement of phytohormonal signaling pathways and their interactions during defense responses against caterpillars or aphids at different densities, when feeding alone or simultaneously on the model plant A. thaliana. The studies show that aphids at different densities interfere in contrasting ways with caterpillar-induced defenses, which required both SA- and JA-signal-transduction pathways. Transcriptional analysis revealed that expression of the SA transcription factor gene WRKY70 was differentially affected upon infestation by aphids at low or high densities. Interestingly, the expression data indicated that a lower expression level of WRKY70 led to significantly higher MYC2 expression through SA-JA crosstalk. Based on these findings, it is proposed that by down-regulating WRKY70 expression, the plant activates JA-dependent defenses which could lead to a higher resistance against aphids and caterpillars. Plutella xylostella caterpillars also influenced plant defense responses when feeding simultaneously with aphids. Caterpillar feeding affected aphid-induced defenses which had negative consequences for aphid performance. Induction of both ET- and JA-mediated defense responses is required for this interference. Moreover, aphid density also played an important role in the modulation by P. xylostella of aphid-induced defenses: P. xylostella caterpillars induced changes in levels of JA and its biologically active from, JA-Ile, only when feeding simultaneously with aphids at a high density. To study the overall effect of dual herbivory on induced plant defenses, not only interference with induced direct defense, but also with induced indirect defenses was addressed in A. thaliana. We found a significant preference of the aphid parasitoid Diaeretiella rapae for volatiles from aphid-infested A. thaliana wild-type plants and ein2-1 (ET-insensitive) mutants. Interestingly, simultaneous feeding by P. xylostella caterpillars on wild-type plants increased D. rapae’s preference for odors from aphid-infested plants. However, upon disruption of the ET-signaling pathway, D. rapae did not distinguish between ein2-1 mutants infested by aphids or by both aphids and caterpillars. This showed that intact ET signaling is needed for caterpillar modulation of the attraction of D. rapae parasitoids. On the other hand, attraction of the caterpillar parasitoid Diadegma semiclausum to volatiles emitted by A. thaliana plants simultaneously infested by caterpillars and aphids was influenced by the density of the feeding aphids. Biosynthesis and emission of the terpene (E,E)-α-farnesene could be linked to the observed preference of D. semiclausum parasitoids for the HIPV blend emitted by plants dually infested by caterpillars and aphids at a high density, compared to dually infested plants with a low aphid density. Transcriptomic changes in the response of A. thaliana wild-type plants to simultaneous feeding by P. xylostella caterpillars and B. brassicae aphids compared to plants infested by P. xylostella caterpillars alone were assessed using a microarray analysis. I particularly addressed the question whether the transcriptomic response to simultaneously attacking aphids and caterpillars was dependent on aphid density and time since initiation of herbivory. The data show that in response to simultaneous feeding by P. xylostella caterpillars and B. brassicae aphids the number of differentially expressed genes was higher compared to plants on which caterpillars had been feeding alone. Additionally, specific genes were differentially expressed in response to aphids feeding at low or high density. Cluster analysis showed that the pattern of gene expression over the different time points in response to dual infestation was also affected by the density of the attacking aphids. These results suggest that insects attacking at a high density cause an acceleration in plant responses compared to insects attacking at low density. As a next step in the study of multiple interacting herbivores, I studied whether plant responses to dual herbivory have consequences for the performance of a subsequently arriving herbivore, Mamestra brassicae caterpillars. The ecological consequences of plant responses to dual herbivory cascading into a chain of interactions affecting other community members have remained unstudied so far. We used wild B. oleracea plants to evaluate dual herbivore-induced plant adaptations for subsequent herbivory. We found that simultaneous feeding by P. xylostella and B. brassicae resulted in different plant defense-related gene expression and differences in plant hormone levels compared to single herbivory, and this had a negative effect on subsequently arriving M. brassicae caterpillars. Differential induction of JA-regulated transcriptional responses to dual insect attack was observed which could have mediated a decrease in M. brassicae performance. The induction of plant defense signaling also affected both P. xylostella and B. brassicae performance. This study further helps to understand herbivore community build-up in the context of plant-mediated species interactions. Altogether, findings from this thesis reveal a molecular basis underlying plant responses against multiple herbivory and provide insight in plant-mediated interactions between aphids and caterpillars feeding on plants growing in the field or used in agriculture.
- Published
- 2016
10. Mapping moves on Arabidopsis : from natural variation to single genes affecting aphid behaviour
- Author
-
Kloth, K.J., Wageningen University, Marcel Dicke, Harro Bouwmeester, and Maarten Jongsma
- Subjects
insect plant relations ,quantitative traits ,functionele genomica ,feeding behaviour ,voedingsgedrag ,plaagresistentie ,Laboratorium voor Plantenfysiologie ,Laboratory of Entomology ,aphidoidea ,insect pests ,arabidopsis thaliana ,Laboratorium voor Entomologie ,genexpressie ,insectenplagen ,pest resistance ,insect-plant relaties ,016-3933 ,BIOS Applied Metabolic Systems ,gene expression ,genetic mapping ,genetische kartering ,EPS ,functional genomics ,kwantitatieve kenmerken ,Laboratory of Plant Physiology - Published
- 2016
11. Plant responses to multiple herbivory : phenotypic changes and their ecological consequences
- Author
-
Yehua Li, Wageningen University, Marcel Dicke, and Rieta Gols
- Subjects
brevicoryne brassicae ,brassica oleracea ,fenotypen ,insect plant relations ,natural enemies ,natuurlijke vijanden ,plant-herbivoor relaties ,parasitic diseases ,plaagresistentie ,Laboratory of Entomology ,genetische variatie ,plant-herbivore interactions ,parasitoïden ,aphidoidea ,insect pests ,defence mechanisms ,fungi ,phenotypes ,food and beverages ,caterpillars ,biochemical phenomena, metabolism, and nutrition ,Laboratorium voor Entomologie ,insectenplagen ,parasitoids ,pest resistance ,insect-plant relaties ,herbivoor-geinduceerde plantengeuren ,genetic variation ,herbivore induced plant volatiles ,EPS ,rupsen ,verdedigingsmechanismen - Abstract
This thesis explores whether aphid-infestation interferes with the plant response to chewing herbivores and whether this impacts performance and behaviour of individual chewing insect herbivores and their natural enemies, as well as the entire insect community. I investigated this using three wild cabbage populations (Brassica oleracea) that are known to differ in inducible secondary chemistry, to reveal whether patterns were consistent. A literature review on recent developments in the field of plant interactions with multiple herbivores (Chapter 2) addressed how plant traits mediate interactions with various species of the associated insect community and their dynamics. In addition, the mechanisms underlying phenotypic changes in response to different herbivores were discussed from the expression of defence-related genes, phytohormones and secondary metabolites in plants to their effects on the performance and behaviour of individual insects as well as the entire insect community. In Chapter 3, I investigated the effects of early-season infestation by the aphid Brevicoryne brassicae on the composition and dynamics of the entire insect community throughout the season in a garden experiment replicated in two consecutive years. Aphid infestation in the early season only affected a subset of the community, i.e. the natural enemies of aphids, but not the chewing herbivores and their natural enemies. Moreover, the effects were only significant in the first half (June & July), but waned in the second half of the season (August & September). The effect of aphid infestation on the community of natural enemies also varied among the cabbage populations. Chapter 4 investigated the effects of aphid infestation on plant direct defences against chewing herbivores in laboratory experiments by comparing the performance of chewing herbivores and their parasitoids on aphid-infested and aphid-free plants. The performance of the specialist herbivore Plutella xylostella and its parasitoid Diadegma semiclausum was better on plants infested with aphids than on aphid-free plants, whereas the performance of the generalist herbivore Mamestra brassicae and its parasitoid Microplitis mediator was not affected by aphid infestation. These results suggest that aphid induced changes in plant traits may differentially affect the performance of leaf-chewing herbivore species attacking the same host plant, and also varied among the cabbage populations. Chapter 5 examined the effects of B. brassicae aphid infestation on plant indirect defences against chewing herbivores. In a two-choice olfactometer bioassay, preference behaviour for volatiles emitted by plants infested with hosts alone and those emitted by plants infested with aphids and hosts was compared for D. semiclausum and M mediator, larval endoparasitoids of caterpillars of P. xylostella and M. brassicae, respectively. In addition, the headspace volatiles emitted by host-infested and dually-infested plants were collected and analyzed. Co-infestation with aphids differentially affected volatile-mediated foraging behaviour of the two parasitoid species in an infestation period-dependent manner. Diadegma semiclausum preferred dually infested plants over host-infested plants when aphids infested the plants for a short time period, i.e. 7 days, but the volatile preference of D. semiclausum was reversed when aphid infestation was extended to 14 days. In contrast, M. mediator consistently preferred volatiles emitted by the dually-infested plants over those emitted by host-infested plants. The patterns of preference behaviour of the two wasp species were consistent across the three cabbage populations. Interestingly, the emission rate of most volatile compounds was reduced in plants dually-infested with caterpillars and aphids compared to singly-infested with caterpillars. This study showed that aphid infestation increased plant indirect defences against caterpillars, but depended on the aphid infestation period and specific caterpillar-parasitoid association. We hypothesized a negative interference of aphid infestation on plant defences against chewing herbivores based on previously reported SA-JA antagonism. In Chapter 6, we assessed the activation of SA and JA signaling pathways in plants infested by both aphids (B. brassicae) and various caterpillar species (P. xylostella, M. brassicae and Pieris brassicae) in different time sequences by quantifying transcription levels of the SA- and JA-responsive marker genes, PR-1 and LOX respectively. The results did not provide support for SA-JA antagonism. Compared to single infestation with each of the herbivore species, dual infestation with aphid and caterpillars had no interactive effects on the transcription levels of the SA- and JA-responsive maker genes, regardless of the temporal sequence of aphid and caterpillar attack, or the identity of the attacking caterpillar species. The findings of this thesis contribute to our understanding of plant responses to herbivory by insect species belonging to different feeding guilds and their ecological effects on other associated community members. Aphid infestation may interfere with plant direct and indirect defences against leaf-chewing herbivores at the individual species level, but the effects are species-specific and also depend on the infestation period of aphids. Early-season aphid infestation may further affect the composition of the insect community, but the effect is smaller influencing only a subset of the community compared to early infestation by chewing herbivores. The molecular mechanism underlying plant responses to both phloem-feeding and leaf-chewing herbivores are complex and require the investigation of a range of genes involved in JA- and SA-mediated defence signal transduction. Plant interact with multiple herbivores at different levels of biological organization ranging from the subcellular level to the individual and the community level, and an integrated multidisciplinary approach is required to investigate plant-insect interactions.
- Published
- 2016
12. Host-plant resistance to western flower thrips in Arabidopsis
- Abstract
Western flower thrips is a pest on a large variety of vegetable, fruit and ornamental crops. The damage these minute slender insects cause in agriculture through feeding and the transmission of tospoviruses requires a sustainable solution. Host-plant resistance is a cornerstone of Integrated Pest Management (IPM). Plants have many natural defense compounds and morphological features that aid in the protection against herbivorous insects. However, the molecular and physiological aspects that control host-plant resistance to thrips are largely unknown. A novel and powerful tool to study host-plant resistance to insects in natural populations is genome-wide association (GWA) mapping. GWA mapping provides a comprehensive untargeted approach to explore the whole array of plant defense mechanisms. The development of high-throughput phenotyping (HTP) systems is a necessity when large plant panels need to be screened for host-plant resistance to insects. An automated video-tracking platform that could screen large plant panels for host-plant resistance to thrips, and dissect host-plant resistance to thrips in component traits related to thrips behavior, was developed. This phenotyping platform allows the screening for host-plant resistance against thrips in a parallel two-choice setup using EthoVision tracking software. The platform was used to establish host-plant preference of thrips with a large plant population of 345 wild Arabidopsis accessions (the Arabidopsis HapMap population) and the method was optimized with two extreme accessions from this population that differed in resistance to thrips. This method can be a reliable and effective high throughput phenotyping tool to assess host-plant resistance to thrips in large plant populations. EthoAnalysis, a novel software package was developed to improve the analyses of insect behavior. There were several benefits from using EthoAnalysis to analyze EthoVision data. The detailed event statistics that could be extracted fro
- Published
- 2016
13. Mapping moves on Arabidopsis : from natural variation to single genes affecting aphid behaviour
- Published
- 2016
14. Unraveling molecular mechanisms underlying plant defense in response to dual insect attack : studying density-dependent effects
- Abstract
In the field, plants suffer from attack by herbivorous insects. Plants have numerous adaptations to defend against herbivory. Not only do these defense responses reduce performance of the feeding herbivore, they also result in the attraction of natural enemies of herbivores. The majority of studies investigating plant-insect interactions addressed mainly the effects of attack by a single herbivore species on induced plant defenses. However, because plants are members of complex communities, plants are exposed to different insect attackers at the same time. Moreover, attacks by different herbivores interact at different levels of biological organization, ranging from the level of gene expression, phytohormone production and biochemical changes up to the individual level. Effects of plant responses to feeding by two or more herbivore species simultaneously might cascade through the community and thereby affect insect community composition. The induction of plant defense responses is regulated by a network of signaling pathways that mainly involve the phytohormones jasmonic acid (JA), salicylic acid (SA) and ethylene (ET). The signaling pathways of the two phytohormones SA and JA interact antagonistically, whereas JA and ET signaling pathways can interact both synergistically and antagonistically in regulating plant defense responses. In general, JA-mediated signaling underlies defense responses against leaf-chewing herbivores, such as caterpillars, whereas phloem-feeding insects, such as aphids, mainly induce SA-regulated defenses. When caterpillars and aphids simultaneously feed on the same host plant, crosstalk between phytohormonal signaling pathways may affect the regulation of plant defenses. Consequently, multiple insect herbivores feeding on plants interact indirectly through plant-mediated effects. Studies investigating molecular mechanisms underlying interference by multiple attacking insects with induced plant defenses will benefit studies on the ecological
- Published
- 2016
15. Plant responses to multiple herbivory : phenotypic changes and their ecological consequences
- Abstract
This thesis explores whether aphid-infestation interferes with the plant response to chewing herbivores and whether this impacts performance and behaviour of individual chewing insect herbivores and their natural enemies, as well as the entire insect community. I investigated this using three wild cabbage populations (Brassica oleracea) that are known to differ in inducible secondary chemistry, to reveal whether patterns were consistent. A literature review on recent developments in the field of plant interactions with multiple herbivores (Chapter 2) addressed how plant traits mediate interactions with various species of the associated insect community and their dynamics. In addition, the mechanisms underlying phenotypic changes in response to different herbivores were discussed from the expression of defence-related genes, phytohormones and secondary metabolites in plants to their effects on the performance and behaviour of individual insects as well as the entire insect community. In Chapter 3, I investigated the effects of early-season infestation by the aphid Brevicoryne brassicae on the composition and dynamics of the entire insect community throughout the season in a garden experiment replicated in two consecutive years. Aphid infestation in the early season only affected a subset of the community, i.e. the natural enemies of aphids, but not the chewing herbivores and their natural enemies. Moreover, the effects were only significant in the first half (June & July), but waned in the second half of the season (August & September). The effect of aphid infestation on the community of natural enemies also varied among the cabbage populations. Chapter 4 investigated the effects of aphid infestation on plant direct defences against chewing herbivores in laboratory experiments by comparing the performance of chewing herbivores and their parasitoids on aphid-infested and aphid-free plants. The performance of the specialist herbivore Plutella xylostella and its parasit
- Published
- 2016
16. Use compost to increase resistance to plant diseases : robust cultivation system removes the need for disinfection
- Abstract
In biological cultivation every opportunity to control soil-borne diseases is a bonus. The idea of providing compost components that can make the soil more resistant to pests and diseases is therefore logical. But its implementation is difficult. The material is complex.
- Published
- 2016
17. Het waarom en hoe van DuRPh: duurzame resistentie tegen Phytophthora in aardappel door cisgene merkervrije modificatie
- Subjects
cell cloning ,genetic control ,plantenziekteverwekkende schimmels ,genetische transformatie ,rassen (planten) ,environmental impact ,PRI Agrosysteemkunde ,innovations ,Biointeractions and Plant Health ,celkloneren ,Laboratorium voor Plantenveredeling ,duurzaamheid (sustainability) ,pesticiden ,plaagresistentie ,potatoes ,phytophthora infestans ,vermeerderingsmateriaal ,genetic engineering ,aardappelen ,milieueffect ,EPS-4 ,economics ,pesticides ,sustainability ,pest resistance ,Plant Breeding ,plant pathogenic fungi ,varieties ,genetische gewasbescherming ,genetische modificatie ,genetic transformation ,Agrosystems ,propagation materials ,economie ,innovaties ,veredelingsmethoden ,breeding methods - Abstract
Begin 2005 verzocht de interdepartementale Commissie Biotechnologie Wageningen UR een onderzoeksprogramma op te stellen voor een perspectiefvol GMO-project. Dit met het oog op de Nederlandse innovatieagenda en behoud of vergroting van de concurrentiekracht van de Nederlandse economie. Uitgangspunt daarbij was de Integrale Nota Biotechnologie (2000) met als centraal motto het verantwoord en zorgvuldig benutten van kansen ten behoeve van economie en duurzame landbouw. De keuze viel op een 'proof of principle' project van een duurzaam Phytophthoraresistente aardappel vanwege de economische aspecten zoals de kosten van beheersing van de ziekte en het veiligstellen van de winstgevendheid van de pootgoedsector. Milieuaspecten zoals reductie van de emissie van chemicaliën en een verminderd energiegebruik voor de toediening telden eveneens mee alsook het vergroten van de wetenschappelijk innovatiekracht
- Published
- 2007
18. Adaptation of the brown planthopper, Nilaparvata lugens (Sta°l), to resistant rice varieties
- Subjects
insect pests ,rice ,food and beverages ,yeasts ,symbionten ,adaptation ,adaptatie ,PE&RC ,Laboratorium voor Entomologie ,insectenplagen ,pest resistance ,gisten ,endosymbionten ,rijst ,endosymbionts ,oryza sativa ,cultivars ,plaagresistentie ,Laboratory of Entomology ,nilaparvata lugens ,symbionts - Abstract
This thesis examines the three-way interaction between yeast-like symbionts, an insect herbivore [Nilaparvata lugens (Stål)] and its rice (Oryza sativa L.) host, during adaptation of the herbivore to resistant rice varieties. A long-term selection study (20 generations of continuous rearing, ca. 24 months) was conducted with N. lugens populations on four rice varieties (IR22, a susceptible variety and IR65482, IR62, and PTB33, three resistant varieties). Planthopper performance and the abundance of yeast-like symbionts (YLS) were monitored throughout the selection process. N. lugens populations adapted to the resistant varieties as noted by increasing body size and increased egglaying. Xylem feeding was observed as a possible behavioural adaptation of N. lugens: planthoppers on resistant plants had relatively high levels of xylem feeding compared with planthoppers on susceptible plants. Planthoppers selected on resistant varieties, had clear differences in YLS densities that were not related to fitness on the varieties and, therefore, did not support a YLS density-mediated adaptation hypothesis. Furthermore, this study examined whether YLS density affected the capacity of planthoppers to switch between hosts on which they have been selected for several generations (natal plant) to new varieties (exposed plants) under normal YLS densities (symbiotic) and after reduction of YLS densities by heat treatment (aposymbiotic). The results suggested that YLS do not mediate host plant switching in planthoppers as removal of symbionts influenced body weight but not the relative capacity of nymphs to feed on different plants. This study also tested if virulence is acquired by shared feeding sites with virulent and avirulent planthoppers. In the study, planthoppers with varying levels of virulence affected the host plants differently: The most virulent hoppers appeared to suppress rice defences to a greater extent than non-virulent planthoppers. Planthoppers attained highest weights on those plants on which virulent planthoppers had previously fed which suggests that feeding by the virulent planthoppers facilitated subsequent planthopper feeding on the same plant. Our preliminary results indicate that feeding by mixed virulent-avirulent populations could potentially accelerate adaptation by N. lugens to resistant rice varieties. The capacity of virulent and avirulent planthoppers to feed on a range of 24 resistant rice varieties was examined using a series of bioassays. Planthoppers were observed to feed and lay eggs on all the varieties tested, many of which have never been widely deployed in the field. Furthermore, planthoppers selected on resistant varieties often had increased fitness on other resistant varieties, even when these possess different resistance genes. However, there was no strong evidence that once planthoppers have adapted to a resistant variety, they will exhibit fitness costs on other varieties with dissimilar genes. The mechanisms underlying insect virulence are complex and further research on planthopper adaptation is necessary to help conserve genetic resources and prolong the durability of available resistant varieties.
- Published
- 2015
19. Resistente biologische aardappelrassen in de etalage
- Author
-
Sikkema, A. and Lammerts Van Bueren, E.
- Subjects
plant protection ,resistentie van variëteiten ,aardappelen ,gewasbescherming ,resistance breeding ,varietal resistance ,phytophthora ,rassen (planten) ,arable farming ,pest resistance ,Plant Breeding ,Corporate Communications & Marketing ,Laboratorium voor Plantenveredeling ,biologische landbouw ,organic farming ,varieties ,organic plant breeding ,plaagresistentie ,potatoes ,akkerbouw ,biologische plantenveredeling ,resistentieveredeling - Abstract
Er zijn zes nieuwe biologische aardappelrassen gepresenteerd die resistent zijn tegen phytophthora.
- Published
- 2015
20. Adaptation of the brown planthopper, Nilaparvata lugens (Sta°l), to resistant rice varieties
- Author
-
Ferrater, J.B., Wageningen University, Marcel Dicke, F.G. Horgan, and Peter de Jong
- Subjects
insect pests ,rice ,food and beverages ,yeasts ,symbionten ,adaptation ,adaptatie ,PE&RC ,Laboratorium voor Entomologie ,insectenplagen ,pest resistance ,gisten ,endosymbionten ,rijst ,endosymbionts ,oryza sativa ,cultivars ,plaagresistentie ,Laboratory of Entomology ,nilaparvata lugens ,symbionts - Abstract
This thesis examines the three-way interaction between yeast-like symbionts, an insect herbivore [Nilaparvata lugens (Stål)] and its rice (Oryza sativa L.) host, during adaptation of the herbivore to resistant rice varieties. A long-term selection study (20 generations of continuous rearing, ca. 24 months) was conducted with N. lugens populations on four rice varieties (IR22, a susceptible variety and IR65482, IR62, and PTB33, three resistant varieties). Planthopper performance and the abundance of yeast-like symbionts (YLS) were monitored throughout the selection process. N. lugens populations adapted to the resistant varieties as noted by increasing body size and increased egglaying. Xylem feeding was observed as a possible behavioural adaptation of N. lugens: planthoppers on resistant plants had relatively high levels of xylem feeding compared with planthoppers on susceptible plants. Planthoppers selected on resistant varieties, had clear differences in YLS densities that were not related to fitness on the varieties and, therefore, did not support a YLS density-mediated adaptation hypothesis. Furthermore, this study examined whether YLS density affected the capacity of planthoppers to switch between hosts on which they have been selected for several generations (natal plant) to new varieties (exposed plants) under normal YLS densities (symbiotic) and after reduction of YLS densities by heat treatment (aposymbiotic). The results suggested that YLS do not mediate host plant switching in planthoppers as removal of symbionts influenced body weight but not the relative capacity of nymphs to feed on different plants. This study also tested if virulence is acquired by shared feeding sites with virulent and avirulent planthoppers. In the study, planthoppers with varying levels of virulence affected the host plants differently: The most virulent hoppers appeared to suppress rice defences to a greater extent than non-virulent planthoppers. Planthoppers attained highest weights on those plants on which virulent planthoppers had previously fed which suggests that feeding by the virulent planthoppers facilitated subsequent planthopper feeding on the same plant. Our preliminary results indicate that feeding by mixed virulent-avirulent populations could potentially accelerate adaptation by N. lugens to resistant rice varieties. The capacity of virulent and avirulent planthoppers to feed on a range of 24 resistant rice varieties was examined using a series of bioassays. Planthoppers were observed to feed and lay eggs on all the varieties tested, many of which have never been widely deployed in the field. Furthermore, planthoppers selected on resistant varieties often had increased fitness on other resistant varieties, even when these possess different resistance genes. However, there was no strong evidence that once planthoppers have adapted to a resistant variety, they will exhibit fitness costs on other varieties with dissimilar genes. The mechanisms underlying insect virulence are complex and further research on planthopper adaptation is necessary to help conserve genetic resources and prolong the durability of available resistant varieties.
- Published
- 2015
21. Bruine rat resistent tegen rattengif : alternatieven zijn dringend gewenst
- Subjects
rats ,plaagresistentie ,pesticidal action ,Emissie & Mestverwaarding ,Emissions & Manure Valorisation ,Laboratory of Entomology ,Laboratorium voor Entomologie ,ratten ,fauna ,pest resistance ,pesticidenwerking - Abstract
Overlast door ratten wordt sinds jaar en dag tegengegaan met rodenticiden (beter bekend als rattengif). Ze zijn toegelaten voor de bestrijding van deze knaagdieren. Maar wat als bruine ratten niet meer doodgaan van deze middelen en hiervoor dus resistent zijn? Wat weten we allemaal over resistentie bij deze soort? En wat zijn de consequenties hiervan voor de bestrijding van de bruine rat?
- Published
- 2013
22. Adaptation of the brown planthopper, Nilaparvata lugens (Sta°l), to resistant rice varieties
- Abstract
This thesis examines the three-way interaction between yeast-like symbionts, an insect herbivore [Nilaparvata lugens (Stål)] and its rice (Oryza sativa L.) host, during adaptation of the herbivore to resistant rice varieties. A long-term selection study (20 generations of continuous rearing, ca. 24 months) was conducted with N. lugens populations on four rice varieties (IR22, a susceptible variety and IR65482, IR62, and PTB33, three resistant varieties). Planthopper performance and the abundance of yeast-like symbionts (YLS) were monitored throughout the selection process. N. lugens populations adapted to the resistant varieties as noted by increasing body size and increased egglaying. Xylem feeding was observed as a possible behavioural adaptation of N. lugens: planthoppers on resistant plants had relatively high levels of xylem feeding compared with planthoppers on susceptible plants. Planthoppers selected on resistant varieties, had clear differences in YLS densities that were not related to fitness on the varieties and, therefore, did not support a YLS density-mediated adaptation hypothesis. Furthermore, this study examined whether YLS density affected the capacity of planthoppers to switch between hosts on which they have been selected for several generations (natal plant) to new varieties (exposed plants) under normal YLS densities (symbiotic) and after reduction of YLS densities by heat treatment (aposymbiotic). The results suggested that YLS do not mediate host plant switching in planthoppers as removal of symbionts influenced body weight but not the relative capacity of nymphs to feed on different plants. This study also tested if virulence is acquired by shared feeding sites with virulent and avirulent planthoppers. In the study, planthoppers with varying levels of virulence affected the host plants differently: The most virulent hoppers appeared to suppress rice defences to a greater extent than non-virulent planthoppers. Planthoppers attained highest wei
- Published
- 2015
23. Resistente biologische aardappelrassen in de etalage
- Abstract
Er zijn zes nieuwe biologische aardappelrassen gepresenteerd die resistent zijn tegen phytophthora.
- Published
- 2015
24. Bodembacterie helpt plant tegen rupsenvraat
- Abstract
Bodembacteriën die in het wortelmilieu van planten leven, verminderen de vatbaarheid van planten voor rupsenvraat. Dat blijkt uit onderzoek van Wageningse entomologen. In de modelplant Arabidopsis konden ze aantonen dat rhizobacteriën de plant in verhoogde staat van paraatheid brengen.
- Published
- 2015
25. Chloroplast levert rna-insecticide : coloradokevers effectiever bestrijden door rna-interferentie via chloroplasten
- Abstract
Duitse onderzoekers claimen vraat door coloradokevers volledig te kunnen voorkomen door rna-interferentie via chloroplasten te organiseren.
- Published
- 2015
26. Resistente biologische aardappelrassen in de etalage
- Abstract
Er zijn zes nieuwe biologische aardappelrassen gepresenteerd die resistent zijn tegen phytophthora.
- Published
- 2015
27. Induction of indirect plant defense in the context of multiple herbivory : gene transcription, volatile emission, and predator behavior
- Subjects
geïnduceerde resistentie ,mites ,transcriptie ,multitrophic interactions ,mijten ,planten ,induced resistance ,phytoseiulus persimilis ,feeding behaviour ,voedingsgedrag ,predatory mites ,plaagresistentie ,genen ,Laboratory of Entomology ,genes ,tetranychus urticae ,multitrofe interacties ,roofmijten ,plants ,defence mechanisms ,fungi ,food and beverages ,Laboratorium voor Entomologie ,genexpressie ,pest resistance ,phaseolus lunatus ,herbivoor-geinduceerde plantengeuren ,gene expression ,herbivore induced plant volatiles ,EPS ,transcription ,verdedigingsmechanismen - Abstract
Plants live in complex environments and are under constant threat of being attacked by herbivorous arthropods. Consequently plants possess an arsenal of sophisticated mechanisms in order to defend themselves against their ubiquitous attackers. Induced indirect defenses involve the attraction of natural enemies of herbivores, such as predators and parasitoids. Predators and parasitoids use odors emitted by damaged plants that serve as a “cry for help” to find their respective prey or host herbivore. The aim of this thesis was to use a multidisciplinary approach, with focus on molecular and chemical methods, combined with behavioral investigations, to elucidate the mechanisms of plant responses to multiple herbivory that affect a tritrophic system consisting of a plant, an herbivore and a natural enemy. Induced plant defenses are regulated by a network of defense signaling pathways in which phytohormones act as signaling molecules. Accordingly, simulation of herbivory by exogenous application of phytohormones and actual herbivory by the two-spotted spider mite Tetranychus urticae affected transcript levels of a defense gene involved in indirect defense in Lima bean. However, two other genes involved in defense were not affected at the time point investigated. Moreover, application of a low dose of JA followed by minor herbivory by T. urticae spider mites affected gene transcript levels and emissions of plant volatiles commonly associated with herbivory. Only endogenous phytohormone levels of jasmonic acid (JA), but not salicylic acid (SA), were affected by treatments. Nevertheless, the low-dose JA application resulted in a synergistic effect on gene transcription and an increased emission of a volatile compound involved in indirect defense after herbivore infestation. Caterpillar feeding as well as application of caterpillar oral secretion on mechanically inflicted wounds are frequently used to induce plant defense against biting-chewing insects, which is JA-related. Feeding damage by two caterpillar species caused mostly identical induction of gene transcription, but combination of mechanical damage and oral secretions of caterpillars caused differential induction of the transcription of defense genes. Nevertheless, gene transcript levels for plants that subsequently experienced an infestation by T. urticae were only different for a gene potentially involved in direct defense of plants that experienced a single event of herbivory by T. urticae. Indirect defense was not affected. Also sequential induction of plant defense by caterpillar oral secretion and an infestation by T. urticae spider mites did not interfere with attraction of the specialist predatory mite P. persimilis in olfactometer assays. The predator did distinguish between plants induced by spider mites and plants induced by the combination of mechanical damage and caterpillar oral secretion but not between plants with single spider mite infestation and plants induced by caterpillar oral secretion prior to spider mite infestation. The composition of the volatile blends emitted by plants induced by spider mites only or by the sequential induction treatment of caterpillar oral secretion followed by spider mite infestation were similar. Consequently, the induction of plant indirect defense as applied in these experiments was not affected by previous treatment with oral secretion of caterpillars. Moreover, herbivory by conspecific T. urticae mites did not affect gene transcript levels or emission of volatiles of plants that experienced two bouts of herbivore attack by conspecific spider mites compared to plants that experienced only one bout of spider mite attack. This suggests that Lima bean plants do no increase defense in response to sequential herbivory by T. urticae. In conclusion, using a multidisciplinary approach new insights were obtained in the mechanisms of induction of indirect plant defense and tritrophic interactions in a multiple herbivore context, providing helpful leads for future research on plant responses to multiple stresses.
- Published
- 2014
28. Genetics of insect resistance to plant defence
- Author
-
Vermeer, K.M.C.A., Wageningen University, Marcel Dicke, and Peter de Jong
- Subjects
insect pests ,genetic resistance ,plants ,secondary metabolites ,defence mechanisms ,phyllotreta nemorum ,planten ,genetic analysis ,genetisch bepaalde resistentie ,insect plant relations ,PE&RC ,Laboratorium voor Entomologie ,insectenplagen ,barbarea vulgaris ,pest resistance ,secundaire metabolieten ,co-evolutie ,insect-plant relaties ,genetische analyse ,coevolution ,plaagresistentie ,Laboratory of Entomology ,verdedigingsmechanismen - Abstract
Plants are chemically defended against insect herbivory in various ways. They produce a broad range of secondary metabolites that may be toxic or deterrent to insects. Specialist insects, however, are often capable of overcoming these defences. The yellow striped flea beetle (Phyllotreta nemorumL.) is a specialist that feeds on crucifers (Brassicaceae) such as Sinapis arvensisand Barbarea vulgaris. In Denmark, two types of Barbarea vulgarisvar. arcuataare distinguished: one with pubescent leaves (P-type) and one with glabrous leaves (G-type). All individuals of P. nemorumcan feed on B. vulgarisP-type. Barbarea vulgarisG-type, on the other hand, is chemically defended against most P. nemorumindividuals during the flea beetle reproductive season. The defence compounds are hypothesized to be saponins, a class of compounds with various biological effects and insecticidal properties. Despite high levels of these saponins during summer, some flea beetles can and do feed on B. vulgaris G-type. The ability of P. nemorumto feed on B. vulgarisG-type is heritable; resistance against the defence of B. vulgarisG-type is controlled by dominant major resistance genes (R-genes). One dominant R-allele of an R-gene is enough to convert a susceptible beetle into a resistant one. Despite knowledge of the inheritance patterns of resistance in the flea beetles, which have been demonstrated to be variable, the underlying mechanism of flea beetle resistance has, so far, remained unclear. This prompted me to investigate, as an initial part of my thesis, the genetic basis of the flea beetle adaptation to the defence of B. vulgarisG-type. The interaction between B. vulgarisand the flea beetle is a unique natural model system to study chemical defences in plants and counter-adaptations in insects. Plant and insect are both polymorphic with respect to the trait involved in resistance and hereby provide an excellent opportunity to study the geographic aspects of the evolution of the resistance trait in both interacting species. In this thesis, I focus on the resistance of the flea beetle, and take the presence of different genotypes of the plant as a given. Phyllotreta nemorumis a major pest, for example in oil seed rape. Understanding how resistance evolves in P. nemorumwill not only benefit flea beetle control, but also control of other pest insects. Understanding insect resistance includes knowledge of seasonal, geographic and genetic variation in both plant defense and herbivore adaptation.The R-gene has a remarkable distribution. Flea beetle populations living on B. vulgarisG-type consist solely of resistant individuals, but on host plant patches nearby B. vulgarisG-type lower frequencies of resistant beetles are found than one would expect with the amount of gene flow found at the neutral level between these subpopulations. The aim of this thesis was to find the gene that is held responsible for the resistance of P. nemorumto the defences of B. vulgaris, investigate the distribution of this resistance trait and explain the distribution of this trait in natural populations. The following questions were addressed: (1) what is the genetic basis of the adaptation under study? (2) how is the resistance distributed across flea beetle populations in Denmark? and (3) which factors underlie this distribution? In order to answer these questions, I used an integrated approach. I have combined a candidate gene approach (CHAPTER3) with an empirical approach via the study of variation in resistance in flea beetle populations (CHAPTER4), and a population genomics approach by using molecular markers to gain insight in the genomic make-up of the population and its connection with the resistance trait (CHAPTERS5 and 6). The population genomics approach is a recent advance in methods to detect the involvement of selection in the distribution of alleles at presumably adaptive loci. Using this approach one can distinguish locus-specific effects, like directional selection, from genome-wide effects, on the distribution of alleles at loci of interest. The population genomics approach is introduced in CHAPTER2 together with the Geographic Mosaic Theory of Coevolution. I illustrate how processes underlying this theory of coevolution can be investigated with the population genomics approach. According to the geographic mosaic theory of coevolution, reciprocal selection between interacting species only happens in so-called hot-spots. Hot spots can be identified using population genomics and genetic variation found at specific loci can be attributed to locus-specific processes such as directional selection. For the B. vulgaris- flea beetle system this means that with a population genomics approach we can examine whether the distribution of resistant flea beetles on alternative host plants is only influenced by migration, or also by selection (CHAPTER5). Another valuable utility of the population genomics approach is to investigate whether a candidate gene for the R-gene is under selection, by looking whether a candidate gene is experiencing locus-specific effects beside genome-wide effects when comparing flea beetle populations living on B. vulgarisG-type with populations living on alternative host plants (CHAPTER6). However, before using a population genomics approach to compare the resistance trait or a candidate gene with parts of the genome that only experience genome-wide effects, I have tried to identify the genetic basis of the flea beetle adaptation to the defence ofB. vulgarisG-type. In CHAPTER3, I have addressed this question by using a candidate gene approach to examine the involvement of a possible detoxifying enzyme in P. nemorum. Genes coding for β-glucosidase were a candidate for genes underlying the difference between resistant and susceptible beetles, because β-glucosidase is used as detoxifying enzyme by other organisms resistant to saponin defence. Three different β-glucosidase cDNA sequences were cloned from Danish flea beetle lines. We named them β-glucosidase A, B and C. β-glucosidase C was only found in resistant lines and not in the susceptible line. We then tested if recombinant β-glucosidase C breaks down the most abundant and most effective defence compound in B. vulgarisG-type, hederagenin cellobioside. β-glucosidase C was able to deglycosylate one glucose unit of hederagenin cellobioside, when expressed in an insect cell line. This suggests that expressed β-glucosidase C can deglycosylate antifeedant saponins and may play a role in the resistant flea beetle’s ability to overcome the defence of B. vulgaris. Next, a segregating family was created in which offspring differed in resistance genotype. Again β-glucosidase cDNA sequences were cloned to find a difference in the presence of these β-glucosidases between resistant and susceptible individuals. This time cDNA sequences of β-glucosidases A, B and C were present in both resistant and susceptible individuals although significantly fewer β-glucosidase C cDNA sequence variants were found in susceptible individuals than in resistant individuals. Thus, the genetic basis of flea beetle resistance remains unclear. Further investigation is needed to explore if the β-glucosidase C protein is also capable of inactivating hederagenin cellobioside by hydrolysizing the second glucose unit from the saponin and if there is a difference in enzyme activity of β-glucosidase C between resistant and susceptible beetles. Subsequently, in CHAPTER4 I have investigated whether the frequency of resistant beetles decreased in populations living on other host plant patches than B. vulgarisG-type and whether the change in frequency was significant within the flea beetle season. I found that the frequency of resistant beetles varied significantly among years, but there was no evidence for a decrease in the frequency of resistant beetles, the latter being expected if selection acts against the resistance on other host plants than B. vulgarisG-type. Furthermore, I found that the frequency of resistant beetles varied significantly within a flea beetle season. This study demonstrates that relative frequencies of different resistance phenotypes of P. nemorumon other host plants than B. vulgarisG-type are highly dynamic, both within and across years. It is, therefore, important to sample season-wide when one wants to monitor the changes in frequencies of insect resistance in natural systems. In CHAPTERS5 and 6 I took a population genomics approach to investigate if the observed geographicaldistribution of resistance of P. nemorumto chemically defendedB. vulgarisin flea beetle populations could be explained by factors that are solely associated with genome-wide effects, such as migration, or also by locus-specific factors like selection at the resistance locus. First, neutral microsatellites were used to reveal the genetic differentiation at parts of the genome that are only influenced by genome-wide processes. Next, the level of neutral genetic differentiation was compared with the genetic differentiation found for the resistance trait. The resistance trait was an outlier in pairwise comparisons between flea beetle populations on B. vulgarisand S. arvensis, meaning that the level of genetic differentiation was significantly higher than expected if the resistance trait experiences only genome-wide effects. The resistance trait was also an outlier in the pairwise comparison between populations on S. arvensis, which suggests that the resistance trait is also under directional selection on other host plants than B. vulgarisG-type. Additionally, I examined in CHAPTER6 if the homologous β-glucosidases B and C sequences found in CHAPTER3 correspond to two alleles of the major resistance gene, because of their similarity and their presence in flea beetle lines. The sequence of β-glucosidases C had so far only been found in resistant individuals, so we hypothesized it to be the dominant resistance allele and the sequence of β-glucosidases B would then correspond to the susceptible allele. In order to find out if this hypothesized PneR-gene (Phyllotreta nemorum R-gene) is the resistance gene, we first directly compared resistance phenotypes of beetles collected from populations on B. vulgarisG-type and S. arvensiswith genotypes derived with primers developed for β-glucosidase B and C. The phenotype of the flea beetles did not match the genotype derived with the β-glucosidase primers. Additionally, the frequency of heterozygotes and homozygotes of the PneR-gene genotype was not significantly deviating from Hardy-Weinberg Equilibrium which implies that there are no locus-specific effects involved when both sequences are seen as one gene with two alleles. A population approach was taken like in CHAPTER5, this time including the genetic differentiation estimated for the candidate gene as well. The candidate gene behaved similar to the neutral loci while the resistance trait was an outlier in most pairwise comparisons between flea beetle populations. If both sequences are alleles of the same gene, then the candidate gene is not directly responsible for the flea beetle resistance to B. vulgarisG-type defence. The results presented in this thesis show the complexity of genetic processes (either genome-wide or locus specific) affecting local adaptation and the distribution of a resistance trait in insects in natural populations. Furthermore, the present study shows that when studying coevolution between insect and host plant by means of adaptive traits, also geographical and seasonal variation in allele frequencies should be considered. A multidisciplinary approach to study adaptation in plant-insect interactions such as used in this thesis, will benefit research on plant-insect interactions, including applied research such as studying the potential of host plants as dead-end traps for pest insects and preventing/diminishing the development of resistance by pest insects to crop defences.
- Published
- 2014
29. Resistentie toets van aardappel tegen Meloidogyne Chitwoodi 2010-2013 : overzicht van het onderzoek medegefinancierd door Productschap Akkerbouw
- Author
-
Molendijk, L.P.G. and Been, T.H.
- Subjects
resistentie van variëteiten ,meloidogyne chitwoodi ,aardappelen ,detectie ,Toegepaste Ecologie ,detection ,netherlands ,varietal resistance ,arable farming ,pest resistance ,OT Team Bedrijfssyst.onderz./Bodemkwaliteit ,nederland ,solanum tuberosum ,plaagresistentie ,potatoes ,akkerbouw ,Applied Ecology ,plant parasitic nematodes ,plantenparasitaire nematoden - Published
- 2014
30. Genetics of insect resistance to plant defence
- Subjects
insect pests ,genetic resistance ,plants ,secondary metabolites ,defence mechanisms ,phyllotreta nemorum ,planten ,genetic analysis ,genetisch bepaalde resistentie ,insect plant relations ,PE&RC ,Laboratorium voor Entomologie ,insectenplagen ,barbarea vulgaris ,pest resistance ,secundaire metabolieten ,co-evolutie ,insect-plant relaties ,genetische analyse ,coevolution ,plaagresistentie ,Laboratory of Entomology ,verdedigingsmechanismen - Abstract
Plants are chemically defended against insect herbivory in various ways. They produce a broad range of secondary metabolites that may be toxic or deterrent to insects. Specialist insects, however, are often capable of overcoming these defences. The yellow striped flea beetle (Phyllotreta nemorumL.) is a specialist that feeds on crucifers (Brassicaceae) such as Sinapis arvensisand Barbarea vulgaris. In Denmark, two types of Barbarea vulgarisvar. arcuataare distinguished: one with pubescent leaves (P-type) and one with glabrous leaves (G-type). All individuals of P. nemorumcan feed on B. vulgarisP-type. Barbarea vulgarisG-type, on the other hand, is chemically defended against most P. nemorumindividuals during the flea beetle reproductive season. The defence compounds are hypothesized to be saponins, a class of compounds with various biological effects and insecticidal properties. Despite high levels of these saponins during summer, some flea beetles can and do feed on B. vulgaris G-type. The ability of P. nemorumto feed on B. vulgarisG-type is heritable; resistance against the defence of B. vulgarisG-type is controlled by dominant major resistance genes (R-genes). One dominant R-allele of an R-gene is enough to convert a susceptible beetle into a resistant one. Despite knowledge of the inheritance patterns of resistance in the flea beetles, which have been demonstrated to be variable, the underlying mechanism of flea beetle resistance has, so far, remained unclear. This prompted me to investigate, as an initial part of my thesis, the genetic basis of the flea beetle adaptation to the defence of B. vulgarisG-type. The interaction between B. vulgarisand the flea beetle is a unique natural model system to study chemical defences in plants and counter-adaptations in insects. Plant and insect are both polymorphic with respect to the trait involved in resistance and hereby provide an excellent opportunity to study the geographic aspects of the evolution of the resistance trait in both interacting species. In this thesis, I focus on the resistance of the flea beetle, and take the presence of different genotypes of the plant as a given. Phyllotreta nemorumis a major pest, for example in oil seed rape. Understanding how resistance evolves in P. nemorumwill not only benefit flea beetle control, but also control of other pest insects. Understanding insect resistance includes knowledge of seasonal, geographic and genetic variation in both plant defense and herbivore adaptation.The R-gene has a remarkable distribution. Flea beetle populations living on B. vulgarisG-type consist solely of resistant individuals, but on host plant patches nearby B. vulgarisG-type lower frequencies of resistant beetles are found than one would expect with the amount of gene flow found at the neutral level between these subpopulations. The aim of this thesis was to find the gene that is held responsible for the resistance of P. nemorumto the defences of B. vulgaris, investigate the distribution of this resistance trait and explain the distribution of this trait in natural populations. The following questions were addressed: (1) what is the genetic basis of the adaptation under study? (2) how is the resistance distributed across flea beetle populations in Denmark? and (3) which factors underlie this distribution? In order to answer these questions, I used an integrated approach. I have combined a candidate gene approach (CHAPTER3) with an empirical approach via the study of variation in resistance in flea beetle populations (CHAPTER4), and a population genomics approach by using molecular markers to gain insight in the genomic make-up of the population and its connection with the resistance trait (CHAPTERS5 and 6). The population genomics approach is a recent advance in methods to detect the involvement of selection in the distribution of alleles at presumably adaptive loci. Using this approach one can distinguish locus-specific effects, like directional selection, from genome-wide effects, on the distribution of alleles at loci of interest. The population genomics approach is introduced in CHAPTER2 together with the Geographic Mosaic Theory of Coevolution. I illustrate how processes underlying this theory of coevolution can be investigated with the population genomics approach. According to the geographic mosaic theory of coevolution, reciprocal selection between interacting species only happens in so-called hot-spots. Hot spots can be identified using population genomics and genetic variation found at specific loci can be attributed to locus-specific processes such as directional selection. For the B. vulgaris- flea beetle system this means that with a population genomics approach we can examine whether the distribution of resistant flea beetles on alternative host plants is only influenced by migration, or also by selection (CHAPTER5). Another valuable utility of the population genomics approach is to investigate whether a candidate gene for the R-gene is under selection, by looking whether a candidate gene is experiencing locus-specific effects beside genome-wide effects when comparing flea beetle populations living on B. vulgarisG-type with populations living on alternative host plants (CHAPTER6). However, before using a population genomics approach to compare the resistance trait or a candidate gene with parts of the genome that only experience genome-wide effects, I have tried to identify the genetic basis of the flea beetle adaptation to the defence ofB. vulgarisG-type. In CHAPTER3, I have addressed this question by using a candidate gene approach to examine the involvement of a possible detoxifying enzyme in P. nemorum. Genes coding for β-glucosidase were a candidate for genes underlying the difference between resistant and susceptible beetles, because β-glucosidase is used as detoxifying enzyme by other organisms resistant to saponin defence. Three different β-glucosidase cDNA sequences were cloned from Danish flea beetle lines. We named them β-glucosidase A, B and C. β-glucosidase C was only found in resistant lines and not in the susceptible line. We then tested if recombinant β-glucosidase C breaks down the most abundant and most effective defence compound in B. vulgarisG-type, hederagenin cellobioside. β-glucosidase C was able to deglycosylate one glucose unit of hederagenin cellobioside, when expressed in an insect cell line. This suggests that expressed β-glucosidase C can deglycosylate antifeedant saponins and may play a role in the resistant flea beetle’s ability to overcome the defence of B. vulgaris. Next, a segregating family was created in which offspring differed in resistance genotype. Again β-glucosidase cDNA sequences were cloned to find a difference in the presence of these β-glucosidases between resistant and susceptible individuals. This time cDNA sequences of β-glucosidases A, B and C were present in both resistant and susceptible individuals although significantly fewer β-glucosidase C cDNA sequence variants were found in susceptible individuals than in resistant individuals. Thus, the genetic basis of flea beetle resistance remains unclear. Further investigation is needed to explore if the β-glucosidase C protein is also capable of inactivating hederagenin cellobioside by hydrolysizing the second glucose unit from the saponin and if there is a difference in enzyme activity of β-glucosidase C between resistant and susceptible beetles. Subsequently, in CHAPTER4 I have investigated whether the frequency of resistant beetles decreased in populations living on other host plant patches than B. vulgarisG-type and whether the change in frequency was significant within the flea beetle season. I found that the frequency of resistant beetles varied significantly among years, but there was no evidence for a decrease in the frequency of resistant beetles, the latter being expected if selection acts against the resistance on other host plants than B. vulgarisG-type. Furthermore, I found that the frequency of resistant beetles varied significantly within a flea beetle season. This study demonstrates that relative frequencies of different resistance phenotypes of P. nemorumon other host plants than B. vulgarisG-type are highly dynamic, both within and across years. It is, therefore, important to sample season-wide when one wants to monitor the changes in frequencies of insect resistance in natural systems. In CHAPTERS5 and 6 I took a population genomics approach to investigate if the observed geographicaldistribution of resistance of P. nemorumto chemically defendedB. vulgarisin flea beetle populations could be explained by factors that are solely associated with genome-wide effects, such as migration, or also by locus-specific factors like selection at the resistance locus. First, neutral microsatellites were used to reveal the genetic differentiation at parts of the genome that are only influenced by genome-wide processes. Next, the level of neutral genetic differentiation was compared with the genetic differentiation found for the resistance trait. The resistance trait was an outlier in pairwise comparisons between flea beetle populations on B. vulgarisand S. arvensis, meaning that the level of genetic differentiation was significantly higher than expected if the resistance trait experiences only genome-wide effects. The resistance trait was also an outlier in the pairwise comparison between populations on S. arvensis, which suggests that the resistance trait is also under directional selection on other host plants than B. vulgarisG-type. Additionally, I examined in CHAPTER6 if the homologous β-glucosidases B and C sequences found in CHAPTER3 correspond to two alleles of the major resistance gene, because of their similarity and their presence in flea beetle lines. The sequence of β-glucosidases C had so far only been found in resistant individuals, so we hypothesized it to be the dominant resistance allele and the sequence of β-glucosidases B would then correspond to the susceptible allele. In order to find out if this hypothesized PneR-gene (Phyllotreta nemorum R-gene) is the resistance gene, we first directly compared resistance phenotypes of beetles collected from populations on B. vulgarisG-type and S. arvensiswith genotypes derived with primers developed for β-glucosidase B and C. The phenotype of the flea beetles did not match the genotype derived with the β-glucosidase primers. Additionally, the frequency of heterozygotes and homozygotes of the PneR-gene genotype was not significantly deviating from Hardy-Weinberg Equilibrium which implies that there are no locus-specific effects involved when both sequences are seen as one gene with two alleles. A population approach was taken like in CHAPTER5, this time including the genetic differentiation estimated for the candidate gene as well. The candidate gene behaved similar to the neutral loci while the resistance trait was an outlier in most pairwise comparisons between flea beetle populations. If both sequences are alleles of the same gene, then the candidate gene is not directly responsible for the flea beetle resistance to B. vulgarisG-type defence. The results presented in this thesis show the complexity of genetic processes (either genome-wide or locus specific) affecting local adaptation and the distribution of a resistance trait in insects in natural populations. Furthermore, the present study shows that when studying coevolution between insect and host plant by means of adaptive traits, also geographical and seasonal variation in allele frequencies should be considered. A multidisciplinary approach to study adaptation in plant-insect interactions such as used in this thesis, will benefit research on plant-insect interactions, including applied research such as studying the potential of host plants as dead-end traps for pest insects and preventing/diminishing the development of resistance by pest insects to crop defences.
- Published
- 2014
31. Whitefly resistance in tomato: from accessions to mechanisms
- Author
-
Lucatti, A.F., Wageningen University, Richard Visser, Ben Vosman, and Sjaak van Heusden
- Subjects
insect pests ,defence mechanisms ,fungi ,food and beverages ,insect plant relations ,plantenveredeling ,insectenplagen ,pest resistance ,PRI Biodiversity and Breeding ,insect-plant relaties ,Plant Breeding ,Laboratorium voor Plantenveredeling ,wild relatives ,wilde verwanten ,bemisia tabaci ,solanum lycopersicum ,PRI Biodiversiteit en Veredeling ,plant breeding ,plaagresistentie ,tomaten ,EPS ,tomatoes ,verdedigingsmechanismen - Abstract
Tomato (Solanum lycopersicum) is affected by a wide range of biotic stresses, of which Bemisia tabaci is one of the most important.Bemisia tabaci affects tomato directly through phloem sap feeding, and indirectly through its ability to be the vector of a large number of viruses. Different methods are available for whitefly control, and although several biological control agents are used against whiteflies in greenhouse cultivation, chemical control still is an essential component in open field tomato production. Breeding for host plant resistance is considered as one of the most promising methods in insect pest control in crop plants, and especially it is a promising alternative in whitefly control. Resistance to whiteflies was found in several wild relatives of tomato like Solanum peruvianum, S. pennellii, S. habrochaites, S. lycopersicum var. cerasiforme, S. pimpinellifolium andS. galapagense. In spite of previous breeding efforts, whiteflies are still a problem in tomato cultivation. The aim of my research was to identify and understand resistance mechanisms targeting specific stages of the whitefly life cycle in order to provide breeders with tools for developing whitefly resistant varieties. I assessed the natural variation and whitefly resistance in Solanum galapagense and S. cheesmaniae, two wild tomato species endemic to the Galapagos Islands. Previously, Solanum galapagense and S. cheesmaniae were classified as two species based on a morphological species concept, but with molecular markers no clear separation could be made. So far, only a limited number of accessions/populations of S. galapagense and S. cheesmaniae have been evaluated for insect resistance and therefore it was unknown if the insect resistance coincides with the morphological species boundaries. Neither was there any knowledge about the relation between geographical and climatic conditions today on the Galapagos and the occurrence of the two species. We characterized twelve accessions of S. galapagense, 22 of S. cheesmaniae, and as reference one of S. lycopersicum for whitefly resistance using no-choice experiments. Whitefly resistance was found in S. galapagense only and was associated with the presence of relatively high levels of acyl sugars and the presence of glandular trichomes of type I and IV.It is likely that a minimum level of acyl sugars and the presence of glandular trichomes type IV are needed to achieve an effective level of resistance. Genetic fingerprinting using 3316 polymorphic SNP markers did not show a clear differentiation between the two species endemic to the Galapagos. Acyl sugar accumulation as well as the climatic and geographical conditions at the collection sites of the accessions did not follow the morphological species boundaries. Altogether, our results suggest that S. galapagense and S. cheesmaniae might be considered as morphotypes rather than two species and that their co-existence is likely the result of selective pressure. Plants possess several resistance mechanisms acting at different time points during the interaction with herbivorous insect. Before any contact with the insects, plants emit an array of volatile organic compounds that can act as attractant or repellent of insects.Bemisia tabaci use a set of plant-derived cues in the process of host plant selection. It recognizes mainly monoterpenes (p-cymene, γ-terpinene and β-myrcene, α-phellandrene) and sesquiterpenes (7-epizingiberene and R-curcumene). Previously the line FCN93-6-2, which was derived from a cross between a susceptible tomato cultivar (Uco Plata INTA) and S. habrochaites (FCN3-5) was proved to be non-preferred by the greenhouse whitefly Trialeurodes vaporariorum. We identified chemical cues produced by FCN93-6-2 and S. habrochaites that can affect the preference of the whitefly B. tabaci as well as the potential chromosomal region(s) of S. habrochaites harbouring the genes involved in the preference. Two S. habrochaites accessions (CGN1.1561 and in FCN3-5) and the line FCN93-6-2 were non-preferred by B. tabaci when the whiteflies could get in direct contact with the plant and also when the whiteflies were offered olfactory cues only. The non-preference was independent of trichome type IV and of the presence of methyl-ketones but associated to the presence of monoterpenes in lower concentrations. Functional validation of the candidate metabolites and of the different introgressions is still needed. Once the insect has landed on a plant, another set of resistance mechanisms enter into action. We have described a 3.06 Mbp introgression on top of Chromosome 5 (OR-5) from the wild tomato species S. habrochaites (CGN1.1561). For the identification of OR-5, we went from the selection of specific F2 plants to the development of F2BC4S1 and F2BC4S2 families. This introgression was sufficient to reduce whitefly fecundity without an evident effect on whitefly survival. The identification of mechanisms exclusively affecting whitefly fecundity and independent of trichomes type IV opens new doors for resistance breeding to whiteflies that may be especially interesting in greenhouse cultivation combination with natural enemies of the whitefly. As an additional layer of defences, plants can perceive stress signals and respond to them in a specific way through induction of their immune system. This induction can also be triggered by exposing the plants to priming agents like hormones, some xenobiotic chemicals, like benzothiadiazole (BTH), β-aminobutyric acid (BABA), and sugars. Although the effect of priming agents was shown in laboratory and field studies, little is known about the effect of the genetic background of tomato on the extent of the priming, e.g. do genotypes varying in their level of resistance to insects and pathogens respond in the same way to a priming agent. We assessed the effect of selected priming agents on the effectiveness of natural defence in tomato. A set of no-choice and choice bioassays was conducted using tomato genotypes varying in their level of basal resistance to Bemisia tabaci and pathogens. We observed that whitefly survival and oviposition were not affected by the priming treatment in no-choice assays. Overall, in choice assays, fructose treated plants were more preferred by whiteflies than control plants. A genotype specific effect of priming was seen for the line FCN93-6-2. On this tomato line, JA and BABA applications decreased the number of whiteflies, e.g. making them less preferred. In this thesis, I have gone from the screening of wild relatives of tomatoes to in depth characterization of resistance mechanisms. I have identified resistance mechanisms targeting specific stages of the whitefly life cycle, thus providing new tools for breeding durable whitefly resistance in tomato.
- Published
- 2014
32. Resistentie toets van aardappel tegen Meloidogyne Chitwoodi 2010-2013 : overzicht van het onderzoek medegefinancierd door Productschap Akkerbouw
- Subjects
resistentie van variëteiten ,meloidogyne chitwoodi ,aardappelen ,detectie ,detection ,Toegepaste Ecologie ,netherlands ,varietal resistance ,arable farming ,pest resistance ,OT Team Bedrijfssyst.onderz./Bodemkwaliteit ,nederland ,solanum tuberosum ,plaagresistentie ,potatoes ,akkerbouw ,Applied Ecology ,plant parasitic nematodes ,plantenparasitaire nematoden - Published
- 2014
33. Whitefly resistance in tomato: from accessions to mechanisms
- Subjects
insect pests ,defence mechanisms ,fungi ,food and beverages ,insect plant relations ,plantenveredeling ,insectenplagen ,pest resistance ,PRI Biodiversity and Breeding ,insect-plant relaties ,Plant Breeding ,Laboratorium voor Plantenveredeling ,wild relatives ,wilde verwanten ,bemisia tabaci ,solanum lycopersicum ,PRI Biodiversiteit en Veredeling ,plaagresistentie ,tomaten ,EPS ,tomatoes ,verdedigingsmechanismen - Abstract
Tomato (Solanum lycopersicum) is affected by a wide range of biotic stresses, of which Bemisia tabaci is one of the most important.Bemisia tabaci affects tomato directly through phloem sap feeding, and indirectly through its ability to be the vector of a large number of viruses. Different methods are available for whitefly control, and although several biological control agents are used against whiteflies in greenhouse cultivation, chemical control still is an essential component in open field tomato production. Breeding for host plant resistance is considered as one of the most promising methods in insect pest control in crop plants, and especially it is a promising alternative in whitefly control. Resistance to whiteflies was found in several wild relatives of tomato like Solanum peruvianum, S. pennellii, S. habrochaites, S. lycopersicum var. cerasiforme, S. pimpinellifolium andS. galapagense. In spite of previous breeding efforts, whiteflies are still a problem in tomato cultivation. The aim of my research was to identify and understand resistance mechanisms targeting specific stages of the whitefly life cycle in order to provide breeders with tools for developing whitefly resistant varieties. I assessed the natural variation and whitefly resistance in Solanum galapagense and S. cheesmaniae, two wild tomato species endemic to the Galapagos Islands. Previously, Solanum galapagense and S. cheesmaniae were classified as two species based on a morphological species concept, but with molecular markers no clear separation could be made. So far, only a limited number of accessions/populations of S. galapagense and S. cheesmaniae have been evaluated for insect resistance and therefore it was unknown if the insect resistance coincides with the morphological species boundaries. Neither was there any knowledge about the relation between geographical and climatic conditions today on the Galapagos and the occurrence of the two species. We characterized twelve accessions of S. galapagense, 22 of S. cheesmaniae, and as reference one of S. lycopersicum for whitefly resistance using no-choice experiments. Whitefly resistance was found in S. galapagense only and was associated with the presence of relatively high levels of acyl sugars and the presence of glandular trichomes of type I and IV.It is likely that a minimum level of acyl sugars and the presence of glandular trichomes type IV are needed to achieve an effective level of resistance. Genetic fingerprinting using 3316 polymorphic SNP markers did not show a clear differentiation between the two species endemic to the Galapagos. Acyl sugar accumulation as well as the climatic and geographical conditions at the collection sites of the accessions did not follow the morphological species boundaries. Altogether, our results suggest that S. galapagense and S. cheesmaniae might be considered as morphotypes rather than two species and that their co-existence is likely the result of selective pressure. Plants possess several resistance mechanisms acting at different time points during the interaction with herbivorous insect. Before any contact with the insects, plants emit an array of volatile organic compounds that can act as attractant or repellent of insects.Bemisia tabaci use a set of plant-derived cues in the process of host plant selection. It recognizes mainly monoterpenes (p-cymene, γ-terpinene and β-myrcene, α-phellandrene) and sesquiterpenes (7-epizingiberene and R-curcumene). Previously the line FCN93-6-2, which was derived from a cross between a susceptible tomato cultivar (Uco Plata INTA) and S. habrochaites (FCN3-5) was proved to be non-preferred by the greenhouse whitefly Trialeurodes vaporariorum. We identified chemical cues produced by FCN93-6-2 and S. habrochaites that can affect the preference of the whitefly B. tabaci as well as the potential chromosomal region(s) of S. habrochaites harbouring the genes involved in the preference. Two S. habrochaites accessions (CGN1.1561 and in FCN3-5) and the line FCN93-6-2 were non-preferred by B. tabaci when the whiteflies could get in direct contact with the plant and also when the whiteflies were offered olfactory cues only. The non-preference was independent of trichome type IV and of the presence of methyl-ketones but associated to the presence of monoterpenes in lower concentrations. Functional validation of the candidate metabolites and of the different introgressions is still needed. Once the insect has landed on a plant, another set of resistance mechanisms enter into action. We have described a 3.06 Mbp introgression on top of Chromosome 5 (OR-5) from the wild tomato species S. habrochaites (CGN1.1561). For the identification of OR-5, we went from the selection of specific F2 plants to the development of F2BC4S1 and F2BC4S2 families. This introgression was sufficient to reduce whitefly fecundity without an evident effect on whitefly survival. The identification of mechanisms exclusively affecting whitefly fecundity and independent of trichomes type IV opens new doors for resistance breeding to whiteflies that may be especially interesting in greenhouse cultivation combination with natural enemies of the whitefly. As an additional layer of defences, plants can perceive stress signals and respond to them in a specific way through induction of their immune system. This induction can also be triggered by exposing the plants to priming agents like hormones, some xenobiotic chemicals, like benzothiadiazole (BTH), β-aminobutyric acid (BABA), and sugars. Although the effect of priming agents was shown in laboratory and field studies, little is known about the effect of the genetic background of tomato on the extent of the priming, e.g. do genotypes varying in their level of resistance to insects and pathogens respond in the same way to a priming agent. We assessed the effect of selected priming agents on the effectiveness of natural defence in tomato. A set of no-choice and choice bioassays was conducted using tomato genotypes varying in their level of basal resistance to Bemisia tabaci and pathogens. We observed that whitefly survival and oviposition were not affected by the priming treatment in no-choice assays. Overall, in choice assays, fructose treated plants were more preferred by whiteflies than control plants. A genotype specific effect of priming was seen for the line FCN93-6-2. On this tomato line, JA and BABA applications decreased the number of whiteflies, e.g. making them less preferred. In this thesis, I have gone from the screening of wild relatives of tomatoes to in depth characterization of resistance mechanisms. I have identified resistance mechanisms targeting specific stages of the whitefly life cycle, thus providing new tools for breeding durable whitefly resistance in tomato.
- Published
- 2014
34. Induction of indirect plant defense in the context of multiple herbivory : gene transcription, volatile emission, and predator behavior
- Author
-
Menzel, T.R., Wageningen University, Marcel Dicke, and Joop van Loon
- Subjects
geïnduceerde resistentie ,mites ,transcriptie ,multitrophic interactions ,mijten ,planten ,induced resistance ,phytoseiulus persimilis ,feeding behaviour ,voedingsgedrag ,predatory mites ,plaagresistentie ,genen ,Laboratory of Entomology ,genes ,tetranychus urticae ,multitrofe interacties ,roofmijten ,plants ,defence mechanisms ,fungi ,food and beverages ,Laboratorium voor Entomologie ,genexpressie ,pest resistance ,phaseolus lunatus ,herbivoor-geinduceerde plantengeuren ,gene expression ,herbivore induced plant volatiles ,EPS ,transcription ,verdedigingsmechanismen - Abstract
Plants live in complex environments and are under constant threat of being attacked by herbivorous arthropods. Consequently plants possess an arsenal of sophisticated mechanisms in order to defend themselves against their ubiquitous attackers. Induced indirect defenses involve the attraction of natural enemies of herbivores, such as predators and parasitoids. Predators and parasitoids use odors emitted by damaged plants that serve as a “cry for help” to find their respective prey or host herbivore. The aim of this thesis was to use a multidisciplinary approach, with focus on molecular and chemical methods, combined with behavioral investigations, to elucidate the mechanisms of plant responses to multiple herbivory that affect a tritrophic system consisting of a plant, an herbivore and a natural enemy. Induced plant defenses are regulated by a network of defense signaling pathways in which phytohormones act as signaling molecules. Accordingly, simulation of herbivory by exogenous application of phytohormones and actual herbivory by the two-spotted spider mite Tetranychus urticae affected transcript levels of a defense gene involved in indirect defense in Lima bean. However, two other genes involved in defense were not affected at the time point investigated. Moreover, application of a low dose of JA followed by minor herbivory by T. urticae spider mites affected gene transcript levels and emissions of plant volatiles commonly associated with herbivory. Only endogenous phytohormone levels of jasmonic acid (JA), but not salicylic acid (SA), were affected by treatments. Nevertheless, the low-dose JA application resulted in a synergistic effect on gene transcription and an increased emission of a volatile compound involved in indirect defense after herbivore infestation. Caterpillar feeding as well as application of caterpillar oral secretion on mechanically inflicted wounds are frequently used to induce plant defense against biting-chewing insects, which is JA-related. Feeding damage by two caterpillar species caused mostly identical induction of gene transcription, but combination of mechanical damage and oral secretions of caterpillars caused differential induction of the transcription of defense genes. Nevertheless, gene transcript levels for plants that subsequently experienced an infestation by T. urticae were only different for a gene potentially involved in direct defense of plants that experienced a single event of herbivory by T. urticae. Indirect defense was not affected. Also sequential induction of plant defense by caterpillar oral secretion and an infestation by T. urticae spider mites did not interfere with attraction of the specialist predatory mite P. persimilis in olfactometer assays. The predator did distinguish between plants induced by spider mites and plants induced by the combination of mechanical damage and caterpillar oral secretion but not between plants with single spider mite infestation and plants induced by caterpillar oral secretion prior to spider mite infestation. The composition of the volatile blends emitted by plants induced by spider mites only or by the sequential induction treatment of caterpillar oral secretion followed by spider mite infestation were similar. Consequently, the induction of plant indirect defense as applied in these experiments was not affected by previous treatment with oral secretion of caterpillars. Moreover, herbivory by conspecific T. urticae mites did not affect gene transcript levels or emission of volatiles of plants that experienced two bouts of herbivore attack by conspecific spider mites compared to plants that experienced only one bout of spider mite attack. This suggests that Lima bean plants do no increase defense in response to sequential herbivory by T. urticae. In conclusion, using a multidisciplinary approach new insights were obtained in the mechanisms of induction of indirect plant defense and tritrophic interactions in a multiple herbivore context, providing helpful leads for future research on plant responses to multiple stresses.
- Published
- 2014
35. Innovaties in de beheersing van plagen
- Subjects
genetic engineering ,plant protection ,disease resistance ,feromonen ,gewasbescherming ,biological control ,biologische bestrijding ,pest resistance ,attractants ,insecten ,Biointeractions and Plant Health ,onderzoeksprojecten ,ziekteresistentie ,lokstoffen ,research projects ,genetische modificatie ,plaagresistentie ,insects ,pheromones - Abstract
Gewasbescherming publiceert een serie artikelen over de verschillende DLO-PO onderzoekprogramma's. In dit artikel aandacht voor signalering en beheersing van plaaginsecten, mijten en slakken. Rubrieken in dit artikel: signaalstoffen, biologische bestrijding en trangene resistente gewassen
- Published
- 2001
36. Duurzame ziekteresistentie, een internationaal symposium
- Subjects
disease resistance ,plant pathology ,partial resistance ,plantenveredeling ,PE&RC ,sustainability ,pest resistance ,Laboratorium voor Phytopathologie ,conferences ,ziekteresistentie ,duurzaamheid (sustainability) ,Laboratory of Phytopathology ,plant breeding ,plaagresistentie ,conferenties ,partiële resistentie ,plantenziektekunde - Abstract
Beeld wordt geschets van de drie orden van resistentieveredeling. De 1e orde is veredeling op hoog niveau van veelal monogene resistentie. De 2e orde is veredeling op partiële resistentie, vaak polygeen. De 3e orde is veredeling op verhoogde predatie, competitie of resistentie (a: verbetering van predatie (b.v. door verminderde harigheid van het blad: b) verbetering van competitie (door biota in de rhizosfeer), c: planten die om hulp roepen (b.v. kairomonen die roofmijten lokken, d: systemic acquired resistance, e: induced systemic resistance
- Published
- 2001
37. Vier jaar onderzoek naar de kastanjeziekte
- Subjects
plant protection ,gewasbescherming ,PPO Bloembollen en Bomen ,pseudomonas syringae ,epidemiologie ,plantenziekteverwekkende bacteriën ,aesculus hippocastanum ,pathogens ,plant pathogenic bacteria ,pest resistance ,LEI Regionale Economie en Ruimtegebruik ,LEI Regional economy & land use ,Nursery Stock-Flower Bulbs ,plaagresistentie ,pathogenen ,epidemiology ,LEI Regionale Economie & Ruimtegebruik - Abstract
De afgelopen jaren heeft de kastanjeziekte zich in snel tempo over Nederland verspreid. De ziekte in paardenkastanje komt ook voor in België, Duitsland, Frankrijk en het Verenigd Koninkrijk. Met steun van het ministerie van Landbouw, Natuur en Voedselkwaliteit heeft de werkgroep Aesculaap de afgelopen vier jaar onderzoek naar de kastanjeziekte gecoördineerd. Het onderzoek in 2008 was gebaseerd op aanbevelingen uit de voorgaande jaren. Het rapport Behoud de kastanje deel 3 is daar de neerslag van. Een samenvatting.
- Published
- 2009
38. Biologische bestrijding van plagen : planten zijn doe-het-zelvers (deel 2)
- Subjects
plant protection ,disease resistance ,nuttige organismen ,gewasbescherming ,biological control ,host range ,biologische bestrijding ,insect plant relations ,Laboratorium voor Entomologie ,ecosystemen ,gastheerreeks ,pest resistance ,insecten ,nuttige insecten ,beneficial insects ,insect-plant relaties ,ziekteresistentie ,beneficial organisms ,plaagresistentie ,EPS ,Laboratory of Entomology ,insects ,ecosystems - Abstract
Biologische bestrijding wordt gezien als noodzakelijk hulmiddel voor de plant bij de verdediging tegen natuurlijke vijanden. Onderzoek toont aan dat planten in staat zijn zich te verdedigen. Ze kunnen biologische bestrijders recruteren om zich tegen plaaginsecten te weren. Planten grijpen daarmee duidelijk in de relaties die er bestaan tussen plantenetende en insectenetende insecten en spelen daarmee een rol in de interacties die in ecosystemen bestaan. In dit verband komen in dit artikel de volgende onderwerpen aan de orde: 1) variatie en het belang van onderzoek naar het gedrag van insecteneters; 2) chemische informatie van planten in een voedselnetwerk; 3) het 'hoe' en 'waarom' van SOS-signalen van planten; 4) 'hard roepende' planten en biologische bestrijders met een keurmerk; 5) consequenties voor biologische bestrijding en waardplantresistentie
- Published
- 1999
39. Genetica, chemie en ecologie van een kwalitatief glucosinolaatpolymorfisme in barbarakruid (Barbarea vulgaris)
- Subjects
experimenteel veldonderzoek ,plant protection ,glucosinolaten ,gewasbescherming ,EPS-2 ,field experimentation ,chemotypen ,genetica ,barbarea vulgaris ,pest resistance ,chemotypes ,plant-herbivoor relaties ,plaagresistentie ,genetics ,nematoda ,Laboratory of Nematology ,glucosinolates ,plant-herbivore interactions ,Laboratorium voor Nematologie - Abstract
Op 17 maart 2008 promoveerde Hanneke van Leur aan Wageningen Universiteit op het proefschrift getiteld 'Genetics, chemistry and ecology of a qualitative glucosinolate polymorphism in Barbarea vulgaris). Uit haar onderzoek kan op basis van de gehouden kas- en veldproeven geconcludeerd worden dat de structuur van de glucosinaten significante verschillen veroorzaakt in resistentie tegen verschillende herbivoren. Het is echter niet zo dat het ene chemotype in alle gevallen meer resistent is dan het andere chemotype.
- Published
- 2008
40. Induction of indirect plant defense in the context of multiple herbivory : gene transcription, volatile emission, and predator behavior
- Abstract
Plants live in complex environments and are under constant threat of being attacked by herbivorous arthropods. Consequently plants possess an arsenal of sophisticated mechanisms in order to defend themselves against their ubiquitous attackers. Induced indirect defenses involve the attraction of natural enemies of herbivores, such as predators and parasitoids. Predators and parasitoids use odors emitted by damaged plants that serve as a “cry for help” to find their respective prey or host herbivore. The aim of this thesis was to use a multidisciplinary approach, with focus on molecular and chemical methods, combined with behavioral investigations, to elucidate the mechanisms of plant responses to multiple herbivory that affect a tritrophic system consisting of a plant, an herbivore and a natural enemy. Induced plant defenses are regulated by a network of defense signaling pathways in which phytohormones act as signaling molecules. Accordingly, simulation of herbivory by exogenous application of phytohormones and actual herbivory by the two-spotted spider mite Tetranychus urticae affected transcript levels of a defense gene involved in indirect defense in Lima bean. However, two other genes involved in defense were not affected at the time point investigated. Moreover, application of a low dose of JA followed by minor herbivory by T. urticae spider mites affected gene transcript levels and emissions of plant volatiles commonly associated with herbivory. Only endogenous phytohormone levels of jasmonic acid (JA), but not salicylic acid (SA), were affected by treatments. Nevertheless, the low-dose JA application resulted in a synergistic effect on gene transcription and an increased emission of a volatile compound involved in indirect defense after herbivore infestation. Caterpillar feeding as well as application of caterpillar oral secretion on mechanically inflicted wounds are frequently used to induce plant defense against biting-chewing insects, which is JA
- Published
- 2014
41. Resistentie toets van aardappel tegen Meloidogyne Chitwoodi 2010-2013 : overzicht van het onderzoek medegefinancierd door Productschap Akkerbouw
- Published
- 2014
42. Genetics of insect resistance to plant defence
- Abstract
Plants are chemically defended against insect herbivory in various ways. They produce a broad range of secondary metabolites that may be toxic or deterrent to insects. Specialist insects, however, are often capable of overcoming these defences. The yellow striped flea beetle (Phyllotreta nemorumL.) is a specialist that feeds on crucifers (Brassicaceae) such as Sinapis arvensisand Barbarea vulgaris. In Denmark, two types of Barbarea vulgarisvar. arcuataare distinguished: one with pubescent leaves (P-type) and one with glabrous leaves (G-type). All individuals of P. nemorumcan feed on B. vulgarisP-type. Barbarea vulgarisG-type, on the other hand, is chemically defended against most P. nemorumindividuals during the flea beetle reproductive season. The defence compounds are hypothesized to be saponins, a class of compounds with various biological effects and insecticidal properties. Despite high levels of these saponins during summer, some flea beetles can and do feed on B. vulgaris G-type. The ability of P. nemorumto feed on B. vulgarisG-type is heritable; resistance against the defence of B. vulgarisG-type is controlled by dominant major resistance genes (R-genes). One dominant R-allele of an R-gene is enough to convert a susceptible beetle into a resistant one. Despite knowledge of the inheritance patterns of resistance in the flea beetles, which have been demonstrated to be variable, the underlying mechanism of flea beetle resistance has, so far, remained unclear. This prompted me to investigate, as an initial part of my thesis, the genetic basis of the flea beetle adaptation to the defence of B. vulgarisG-type. The interaction between B. vulgarisand the flea beetle is a unique natural model system to study chemical defences in plants and counter-adaptations in insects. Plant and insect are both polymorphic with respect to the trait involved in resistance and hereby provide an excellent opportunity to study the geographic aspects of the evolution of the resistance t
- Published
- 2014
43. Whitefly resistance in tomato: from accessions to mechanisms
- Abstract
Tomato (Solanum lycopersicum) is affected by a wide range of biotic stresses, of which Bemisia tabaci is one of the most important.Bemisia tabaci affects tomato directly through phloem sap feeding, and indirectly through its ability to be the vector of a large number of viruses. Different methods are available for whitefly control, and although several biological control agents are used against whiteflies in greenhouse cultivation, chemical control still is an essential component in open field tomato production. Breeding for host plant resistance is considered as one of the most promising methods in insect pest control in crop plants, and especially it is a promising alternative in whitefly control. Resistance to whiteflies was found in several wild relatives of tomato like Solanum peruvianum, S. pennellii, S. habrochaites, S. lycopersicum var. cerasiforme, S. pimpinellifolium andS. galapagense. In spite of previous breeding efforts, whiteflies are still a problem in tomato cultivation. The aim of my research was to identify and understand resistance mechanisms targeting specific stages of the whitefly life cycle in order to provide breeders with tools for developing whitefly resistant varieties. I assessed the natural variation and whitefly resistance in Solanum galapagense and S. cheesmaniae, two wild tomato species endemic to the Galapagos Islands. Previously, Solanum galapagense and S. cheesmaniae were classified as two species based on a morphological species concept, but with molecular markers no clear separation could be made. So far, only a limited number of accessions/populations of S. galapagense and S. cheesmaniae have been evaluated for insect resistance and therefore it was unknown if the insect resistance coincides with the morphological species boundaries. Neither was there any knowledge about the relation between geographical and climatic conditions today on the Galapagos and the occurrence of the two species. We characterized twelve accessions of S.
- Published
- 2014
44. Ben Vosman, specialist insectenresistentie bij Wageningen UR: ‘wegvallen insecticiden vraagt om resistent ras’
- Abstract
Hoe houd je met steeds minder middelen plaaginsecten onder de duim? Een deel van de oplossing ligt in minder gevoelige plantenrassen, meent Ben Vosman, specialist insectenresistentie bij Wageningen UR Plant Breeding. „Maar minder afhankelijk worden van chemie vraagt ook wat van de teler.”
- Published
- 2014
45. Inzicht door het 'videotracken' van insecten
- Abstract
Maarten Jongsma is senior onderzoeker en werkt bij Plant Research International (PRI), onderdeel van Wageningen Universiteit en Research Centrum. Hij is moleculair bioloog en is gepromoveerd op 'plant-insectrelaties'. Hij weet veel over hoe planten zich weerbaar maken tegen plagen zoals trips, bladluis en witte vlieg. "Wij bedenken innovatieve tools voor veredelaars om snel resistentie-eigenschappen te vinden."
- Published
- 2014
46. Resistentie en financiële opbrengst bepalen rassenkeuze
- Abstract
Op de aanbevelende rassenlijst 2015 staan negen nieuwe rassen. Samen met de bekende rassen kan de teler in principe kiezen uit in totaal 22 rassen. Echter, centraal bij de keuze staat de resistentie die nodig is. Daarna is de financiële opbrengst bepalend.
- Published
- 2014
47. Op zoek naar de ideale aardappel : introductie van nieuwe generatie resistente aardappelrassen kost veel tijd en moeite
- Abstract
Na jaren van kruizen en selecteren groeit het aanbod van phytophthoraresistente aardappelrassen. De meesten hiervan hebben zich afgelopen jaar in veldproeven bewezen. Nu is het tijd dat deze nieuwe rassen ook een plek in het aardappelschap veroveren. De Groene Winkel in Zeist neemt het voortouw.
- Published
- 2014
48. Bruine rat resistent tegen rattengif : alternatieven zijn dringend gewenst
- Author
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Schoelitsz, B. and Meerburg, B.G.
- Subjects
rats ,plaagresistentie ,pesticidal action ,Emissie & Mestverwaarding ,Emissions & Manure Valorisation ,Laboratory of Entomology ,Laboratorium voor Entomologie ,ratten ,fauna ,pest resistance ,pesticidenwerking - Abstract
Overlast door ratten wordt sinds jaar en dag tegengegaan met rodenticiden (beter bekend als rattengif). Ze zijn toegelaten voor de bestrijding van deze knaagdieren. Maar wat als bruine ratten niet meer doodgaan van deze middelen en hiervoor dus resistent zijn? Wat weten we allemaal over resistentie bij deze soort? En wat zijn de consequenties hiervan voor de bestrijding van de bruine rat?
- Published
- 2013
49. Resistance mechanisms against Bemisia tabaci in wild relatives of tomato
- Subjects
insect pests ,EPS-2 ,defence mechanisms ,food and beverages ,plantenveredeling ,Laboratorium voor Entomologie ,insectenplagen ,pest resistance ,PRI Biodiversity and Breeding ,Plant Breeding ,Laboratorium voor Plantenveredeling ,wild relatives ,wilde verwanten ,bemisia tabaci ,solanum lycopersicum ,PRI Biodiversiteit en Veredeling ,plaagresistentie ,Laboratory of Entomology ,verdedigingsmechanismen - Abstract
The silverleaf whitefly (Bemisia tabaciGenn.) poses a serious threat to tomato cultivation. A large part of the damage is done directly through heavy host plant colonization. Colonization has a negative impact on the plant, as the whitefly takes up nutrients from the phloem and induces phytotoxic responses, which result in irregular ripening of the fruits. However, most damage is done indirectly as the silverleaf whitefly vectors a broad range of plant pathogenic viruses. The silverleaf whitefly can successfully be controlled biologically in greenhouse cultivations, but control of the whitefly in the field is mainly based on the application of pesticides. The use of pesticides can have a negative effect on non-harmful or beneficial organisms in the field. Moreover, the effectiveness of pesticides can decline or even completely disappear through adaptation of the whitefly. An effective alternative for the use of pesticides could be the deployment of resistant cultivars. Nowadays, genetic factors responsible for whitefly resistance can be transferred faster and more efficiently into tomato cultivars through marker-assisted backcross breeding programs. Complete resistance against the whitefly is present in some crossable wild relatives of the cultivated tomato and the literature reports extensively about accessions with a high level of resistance against the whitefly. In this work, I have studied different populations that were developed by interspecific crosses between cultivated tomato and the tomato wild relativesS. habrochaitesLYC4 and S. pennelliiLA3791. By integrating datasets from different research disciplines, I have studied the background of whitefly resistance in these populations. Furthermore, these data were used to identify the chromosomal loci in the wild tomato relatives that harbor genes responsible for the resistance and that can be bred into cultivated tomato. The mechanisms underlying the resistance in S. pennelliiLA3791 were studied through phenotypic resistance assays that demonstrated that survival and oviposition of the whitefly were not possible on this wild relative. Through removal of glandular cells, present on the leaf trichomes, the resistance was almost completely lost and only adult survival was still significantly different from the wild type. This result led to the hypothesis that glandular trichomes play an important role in the resistance. This was confirmed in a segregating population based on a cross between S. pennelliiLA3791 and a susceptible cultivated tomato. Plants that lacked glandular trichomes type I and IV, had the same resistance level as the susceptible parent. Further analyses of the segregating population showed that the presence of glandular trichomes was not the only factor determining resistance, but that the composition and quantity of the metabolites in the glandular trichomes also played an important role. To gain more knowledge on the role of individual metabolites on whitefly resistance and susceptibility, we analyzed the total metabolite content of extreme phenotypes of the F2 population. Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography Time-Of-Flight Mass Spectrometry (LC-TOF-MS) were employed for the analyses of the total metabolite content. Analyses revealed that on basis of the total metabolite profiles the extreme phenotypes (susceptible versus resistant for the silverleaf whitefly) could be discriminated into two groups that were correlated with resistance or susceptibility. A number of these metabolites could be annotated, but for the majority of the components this was not possible on the basis of available literature and databases. Subsequently, I have studied the genetic basis of the phenotypic resistance parameters as well as the genetic basis of the metabolites from the GC-MS and LC-TOF-MS analyses. A genetic linkage map of the F2 mapping population was developed using DNA markers (Amplification Fragment Length Polymorphisms,AFLPs and Single Nucleotide Polymorphisms, SNPs). QTLs (Quantitative Trait Loci) were identified between the majority of the metabolites and the genetic markers (>90%) and also we found genetic linkages between whitefly resistance parameters and markers. The QTLs for metabolites and phenotypic parameters partly co-localized at the same positions on the genetic map. Several metabolite QTLs (mQTLs) co-localized with each other in so-called ‘hotspots’. Remarkably, the results of the individual phenotypic QTLs (phQTLs) for adult survival and oviposition as well as the mQTLs for the individual components did not give high explained variances (
- Published
- 2013
50. Resistance to thrips in pepper
- Subjects
sweet peppers ,ziekten ,interspecific hybridization ,plantenveredeling ,capsicum annuum ,thrips ,Laboratorium voor Plantenveredeling ,plaagresistentie ,spaanse pepers ,capsicum chinense ,paprika's ,vectoren ,chillies ,insect pests ,EPS-2 ,fungi ,food and beverages ,insectenplagen ,pest resistance ,Plant Breeding ,quantitative trait loci ,loci voor kwantitatief kenmerk ,capsicum ,disease vectors ,frankliniella occidentalis ,soortkruising - Abstract
Pepper (Capsicum) production is constrained by heavy infestations of thrips, causing direct and indirect (by transmitting viruses) damage. Thrips control using chemical insecticides, biological agents, culture practices and integrated pest management has limited success. The availability of thrips-resistant varieties would increase the effectiveness of thrips control and may also delay and reduce the transmission of viruses. This thesis is aimed at obtaining more knowledge regarding thrips resistance in pepper, including the identification of new sources of resistance, the elucidation of resistance mechanisms, identification of factors contributing to resistance and a QTL analysis. We developed several test methods to evaluate plant resistance to thrips and showed that in vitro tests correlate well with greenhouse tests. We used these methods to test a collection of Capsicum accessions of widely different origin and crop types. This resulted in the identification of a few accessions (mostly C. annuum) with high levels of resistance to two thrips species: Frankliniella occidentalis and Thrips parvispinus. Since C. annuum is the most widely cultivated species, the finding of resistance in C. annuum is means that the resistance can be easily introgressed through conventional crossing and selection. The effect of resistance in pepper on thrips reproduction and development was studied using three highly resistant, three medium resistant and three susceptible accessions selected based on damage ratings. Adult and pre-adult survival, developmental time and reproduction rate were assessed in a detached leaf system. Resistance factors in leaves of resistant pepper accessions were shown to have significant effects on oviposition rate, larval mortality and life-cycle period, indicating that this resistance is based on antibiosis. In order to map QTL for resistance we developed an F2 population from the cross between a susceptible C. chinense accession and the resistant C. annuum AC 1979. A genetic linkage map for this population was based on AFLP and SSR markers, where the SSR markers served to assign and orient most linkage groups to pepper chromosomes. As larval stages were highly affected by resistance in pepper leaves, damage caused by larvae and larval survival were used as parameters to detect QTLs conferring resistance to thrips. Interval mapping detected one QTL for each of these parameters, all co-localizing near the same marker on chromosome 6. This QTL explained about 50% of the genetic variation, and the resistance allele of this QTL was inherited from the resistant parent. No other resistance QTLs were detected in this population. Since resistance to thrips was clearly expressed in pepper leaves we proceeded to study leaf traits that may contribute to resistance. Morphological leaf characters and metabolites have frequently been linked with resistance to thrips in other plant species. However, we found no convincing evidence that any of these traits played a role in thrips resistance in pepper. In the F2 mapping population we found no correlation and no QTL co-localization of resistance with leaf morphological characters previously linked to resistance in pepper against insect pest and in other plant species against thrips e.g. color, toughness, trichome density, and cuticula thickness. GC-MS (Gass Chromatography – Mass Spectrometry) analysis of the three resistant, three intermediate and three susceptible accessions mentioned above showed that seven metabolites were correlated with resistance to thrips and six compounds with susceptibility. However, when we applied GC-MS and LC-MS (Liquid Chromatography – Mass Spectrometry) to leaves of the F2 mapping population, we found no strong correlation between resistance and any detected metabolites. Two metabolite QTLs co-localized with the resistance QTL. However, these QTLs explained only a small proportion of the variance and the co-localization was not supported by strong correlations of the metabolites with resistance. This suggests that the major resistance factor(s) in pepper against thrips may not or only partially be determined by the presence or absence of specific metabolites. This thesis provides a strong basis for the development of thrips resistant pepper varieties through introgression of the resistance QTL region on chromosome 6 originating from resistant C. annuum accessions. However, the effect of resistance QTL on chromosome 6 should be confirmed in another population such as a population of F3 lines. In vitro leaf assay can be used as evaluation methods in pepper breeding program. This has the advantages of minimizing the risk of contamination and of controlled environmental conditions. Elucidation of factors contributing to resistance should be continued by giving attention to other possibilities such as proteins, specifically proteinase inhibitors, or other leaf anatomical and morphological traits. Also other extraction and detection methods may be used to discover other metabolites that might be related to resistance. Finally, for practical applications it is necessary study how to use the antibiosis based mechanism against thrips found in this thesis in thrips control and/or management practices.
- Published
- 2013
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