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Intensive use of pesticides is among the main drivers of biodiversity loss, especially of insects. Here, field concentrations of chemical synthetic pesticides were measured in soil and carabid beetles in a vineyard (VP) and olive grove (OP), in two consecutive years. The aim was to determine if active ingredients in pesticides applied in the field accumulate in carabids and how this correlates with treatment intensity. Carabids and soil samples were collected at a vineyard and olive grove in Zadar County in Croatia, soil in 2018 and 2019 and carabids in 2019. Both were under integrated pest management (IPM), with a total of 34 pesticides applied, between January and August in the two years of this study. Using LC-MS/MS, a broad range of pesticides, mainly fungicides, was detected in the soil and carabids. In soil samples, boscalid (0.047 mg/kg), mandipropamid (0.08 mg/kg), fluopyram (0.09 mg/kg), cyprodinil (0.09 mg/kg) and tebuconazole (0.13 mg/kg) were detected in the highest amounts. In addition, nine substances were detected in carabids, with valiphenalate (0.048 mg/kg), difenoconazole (0.051 mg/kg) and azoxystrobin (0.064 mg/kg) in the highest concentrations. Bioaccumulation factor (BAF) indicated the accumulation of valiphenalate, metalaxyl-M, spiroxamine and difenoconazole in carabids. Data measured directly in the field revealed the accumulation of pesticides in carabids, which indicates they could be good bioindicators in IPM and contribute to a better understanding of the distribution of pesticides in Mediterranean agroecosystems.

Intensive use of pesticides is among the main drivers of biodiversity loss, especially of insects. Here, field concentrations of chemical synthetic pesticides were measured in soil and carabid beetles in a vineyard (VP) and olive grove (OP), in two consecutive years. The aim was to determine if active ingredients in pesticides applied in the field accumulate in carabids and how this correlates with treatment intensity. Carabids and soil samples were collected at a vineyard and olive grove in Zadar County in Croatia, soil in 2018 and 2019 and carabids in 2019. Both were under integrated pest management (IPM), with a total of 34 pesticides applied, between January and August in the two years of this study. Using LC-MS/MS, a broad range of pesticides, mainly fungicides, was detected in the soil and carabids. In soil samples, boscalid (0.047 mg/kg), mandipropamid (0.08 mg/kg), fluopyram (0.09 mg/kg), cyprodinil (0.09 mg/kg) and tebuconazole (0.13 mg/kg) were detected in the highest amounts. In addition, nine substances were detected in carabids, with valiphenalate (0.048 mg/kg), difenoconazole (0.051 mg/kg) and azoxystrobin (0.064 mg/kg) in the highest concentrations. Bioaccumulation factor (BAF) indicated the accumulation of valiphenalate, metalaxyl-M, spiroxamine and difenoconazole in carabids. Data measured directly in the field revealed the accumulation of pesticides in carabids, which indicates they could be good bioindicators in IPM and contribute to a better understanding of the distribution of pesticides in Mediterranean agroecosystems. [ABSTRACT FROM AUTHOR]

Currently, testing of acute inhalation toxicity in animals is required for regulation of pesticide active ingredients and formulated plant protection products. The main outcome of the regulatory tests is "lethal concentration 50″ (LC 50), i.e. the concentration that will kill 50% of the exposed animals. However, ongoing work aims to identify New Approach Methods (NAMs) to replace animal experiments. To this end, we studied 11 plant protection products, sold in the European Union (EU), for their ability to inhibit lung surfactant function in vitro in the constrained drop surfactometer (CDS). In vivo, inhibition of lung surfactant function can lead to alveolar collapse and reduction of tidal volume. Therefore, we also assessed changes in breathing patterns of mice during exposure to the same products. Six of the eleven products inhibited lung surfactant function, and six products reduced tidal volume in mice. In vitro inhibition of lung surfactant function predicted reduction in tidal volume in exposed mice with a sensitivity of 67% and a specificity of 60%. Two products were labelled as "harmful if inhaled", both inhibited surfactant function in vitro and reduced tidal volume in mice. Lung surfactant function inhibition in vitro predicted reduction in tidal volume for plant protection products to a lesser degree than for previously tested substances. This could owe to the requirement for rigorous testing of plant protection products prior to approval that might have selected against substances that could potentially inhibit lung surfactant, e.g. due to severe adverse effects during inhalation. • Testing of plant protection products requires inhalation toxicity testing in animals. • New approach methods are needed in this field. • We tested effects of plant protection products on lung surfactant function in vitro. • In vitro prediction of in vivo results was 63% - lower than other tested chemicals. • Rigorous testing of these products might select against lung surfactant inhibitors. [ABSTRACT FROM AUTHOR]

Spodoptera litura Fabricius is one of polyphagous insects that often attacks so many plant species either annual and perennial crops. .Severe infestation of the pest may decrease crop production. The farmers who grow agricultural crops generally control the insect pest using synthetic chemical insecticides. However, improper use of such pesticides may cause negative impacts to the environment. Insect pest control using botanical insecticide is an alternative which is relatively safe to the environment. A research to test the lethal effect of crude extract of bintaro leaf (Cerbera odollam gaertn.) to S. litura larvae was conducted. The research was conducted at the Laboratory of Forest Product Chemistry and the Laboratory of Forest Entomology, Faculty of Forestry, IPB in 2009. The results shows that the crude extract of bintaro leaf has a strong knock down effect to S. litura larvae, with LC50 at 0,6 % to the second instar and 0,28 % to the second and the third instar. It is proven that the leaf extract, contains active chemical compounds pesticidal, e.g. flavonoid, steroid, saponin and tannin. Further tests are suggested under field condition researches should be conducted to test the efficacy of the extract in controlling S. litura at the nursery and the field