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The aim of this paper was to define and quantify the most important attributes of entrepreneurs in the segment of small and medium-sized enterprises. The partial objective was to compare the opinions of the entrepreneurs in relation to their gender, age and level of education. Scientific questions, defined in this paper, were verified through empirical research conducted in the Czech Republic in 2015 on a sample of 1,141 entrepreneurs. In our research, we found that entrepreneurs consider expertise, responsibility and perseverance to be the most important personal characteristics and skills. Propensity to risk and decisiveness are ranked right after them. The same group of three most important attributes is presented by all defined groups of entrepreneurs, which means that the assessment of the importance of these attributes remains the same regardless of gender, age and level of education of the entrepreneur. It is interesting that the weight of the expertise increases with the age of the entrepreneur. This trend is caused by the fact that men significantly more intensively presented this opinion. When evaluating the attitudes of entrepreneurs to other characteristics and skills no significant differences in relation to gender, age and education were identified.