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1. Contribution of adipose stem cells from obese subjects to hepato-or breast-carcinoma tumorogenesis, through promotion of Th17 cells

Author

CHEHIMI, Marwa, Delort, Laetitia, Vidal, Hubert, Caldefie-Chezet, Florence, Eljaafari, Assia, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Cancéropôle Lyon Auvergne Rhône-Alpes (CLARA). FRA., ProdInra, Archive Ouverte, Hospices Civils de Lyon (HCL), Centre de Recherche en Nutrition Humaine (CRNH). FRA., Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), and Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université

Subjects
obesity, culture cellulaire, Th17 lymphocytes, cellule humaine, hepatic cancer, cancer du sein, [SDV]Life Sciences [q-bio], obese adipose stem cells, tissu adipeux, breast cancer, mammary malignant tumor, adipose tissue, [SDV] Life Sciences [q-bio], [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, obésité, inflammation, Alimentation et Nutrition, Food and Nutrition, cancer, microbial culture, cancer du foie, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, patient obèse
Abstract

SESSION 4 - BREAST CANCER: FROM EXPERIMENTAL APPROACH TO CLINICAL; IntroductionAs opposed with lean adipose tissues (AT), obese AT are heavily infiltrated with variety of inflammatory cells. Among them, Th17 cells are found not only within AT, but also in the periphery in obese subjects. We have demonstrated that AT-derived stem cells (ASC), or their progenitors, contribute to inflammation through promotion of Th-17 cells, provided that they are issued from obese-, but not lean-AT (Diabetes, 2015; Adipocyte, 2016). Because obesity is associated with increased prevalence of various cancers, including hepatic or breast cancer, we postulated herein that ASC-mediated promotion of Th17 cells might result in tumorogenesis progression.Materials and Methods: Human ASC were isolated from WAT of obese donors (obASC). Mononuclear cells (MNC) werecollected from blood donors. PHA-activated co-cultures of obASC/MNC, which increase secretion of IL-17A, IL-1b and IL-6, were performed. Conditioned media (CM) were collected from such cultures, and added to HuH7 (hepato-carcinoma cell line) or MCF-7 / MDA-MB-231 (breast carcinoma cell line) cultures for 24h. mRNA profiles were measured by qRTPCR. Expression of CXCR4 was measured by flow cytometry. Results: CM from 48 hr PHA-activated-ASC/MNC co-cultures enhanced IL-1b, VEGFa, IL-8 TNFa and IL-6 mRNA expression in HUH7 by almost 700, 2, 3, 3, and 6-fold, respectively. A putative effect of CM on HUH7 invasiveness was supported by 2a –fold, and 3-fold increase in MMP9, and CXCR4 expression, respectively. In addition, CM also increased IL-1b, IL-6, IL-8 and VEGF-a mRNA expression in both MCF-7 and MDA-MB-231 cell lines.Conclusion: Our results suggest that the interaction of ob ASC with immune cells contribute to an inflammatory environment, able to impact hepato- or breast-carcinoma cell secretion profile, and/or invasiveness, either through propagation of inflammatory cytokines outside adipose tissues, or ASC migration inside tumors

Published
2017

2. Leptin induces ROS via NOX5 in healthy and neoplastic mammary epithelial cells

Author

Sophie Ortega, S. Rougé, Marie-Paule Vasson, Adrien Rossary, Sinda Mahbouli, Audrey der Vartanian, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Université Clermont Auvergne (UCA), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Conseil Regional d'Auvergne, and European research fund (FEDER)

Subjects
0301 basic medicine, Leptin, Cancer Research, Cell, Adipokine, Breast Neoplasms, [SDV.CAN]Life Sciences [q-bio]/Cancer, Biology, medicine.disease_cause, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, medicine, Humans, cancer, cellule épithéliale mammaire, RNA, Small Interfering, Mammary Glands, Human, patient obèse, NADPH oxidase, Epithelial Cells, ROS, General Medicine, Cell cycle, stress oxydant, Gene Expression Regulation, Neoplastic, Oxidative Stress, 030104 developmental biology, medicine.anatomical_structure, Oncology, NADPH Oxidase 5, Verapamil, Apoptosis, 030220 oncology & carcinogenesis, biology.protein, Cancer research, MCF-7 Cells, Female, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Reactive Oxygen Species, Oxidative stress
Abstract

NADPH oxidase (NOX) complexes (a family of seven isoforms) drive cellular ROS production in patho-logical processes such as cancer. NOX-driven ROS production is involved in cell mechanisms from signalling to oxidative stress. Leptin, an adipokine overexpressed in obese patients, has been investigated in studies on breast carcinogenesis, but its effects on oxidative stress remain largely unexplored, especially in breast cancer. The study used three human mammary epithelial cell models presenting different neoplastic status (healthy primary HMECs, neoplastic MCF-7 cells and neoplastic MDA-MB-231 cells) to determine the effects of leptin on short-term ROS production and to characterize the enzymes involved. All three cell models significantly expressed NADPH oxidase isoform 5 (NOX5) in our culture conditions. All models showed induced ROS production regardless of leptin concentration (10 ng/ml mimicking good health, 100 ng/ml mimicking obesity). Cell treatment with either siRNA against NOX5, NOX inhibitor DPI or a calcium channel blocker (verapamil) confirmed the putative involvement of the NOX5 isoenzyme in ROS production. Moreover, cell treatments suppressed ROS production under leptin at both concentrations. Neoplastic cells appeared unable to downregulate NOX5 mRNA expression under leptin. Leptin emerged as a potential activator of ROS production in human epithelial mammary cells, where the ROS production was apparently linked to NOX5 activation. This novel finding could shed light on the potential role of obesity-associated hyperleptinemia in mammary cells via the activation of NOX enzymes.

Published
2017

3. A Novel Smartphone Accelerometer Application for Low-Intensity Activity and Energy Expenditure Estimations in Overweight and Obese Adults

Author

Yves Boirie, Sylvie Rousset, Damien Saboul, Martine Duclos, N. Farigon, Clément Lahaye, Ludivine Paris, Romain Guidoux, M. Miolanne, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Service Nutrition Clinique, CHU Clermont-Ferrand, Service Médecine du Sport et des Explorations Fonctionnelles, Almerys, Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM ), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet [Saint-Étienne] (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), almerys 101,923-581, Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Centre Hospitalier Universitaire de Clermont-Ferrand, Service Médecine du Sport et Explorations Fonctionnelles [CHU Clermont-Ferrand], CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand-CHU Clermont-Ferrand-CHU Estaing [Clermont-Ferrand], and Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])

Subjects
Adult, medicine.medical_specialty, Medicine (miscellaneous), Health Informatics, Overweight, Motor Activity, Accelerometer, Obese, 03 medical and health sciences, 0302 clinical medicine, Health Information Management, dépense d'énergie, accelerometry, Medicine, Humans, 030212 general & internal medicine, Obesity, Activity intensity, patient obèse, Mobility, business.industry, Life style, Specific function, 030229 sport sciences, accelerométrie, medicine.disease, Intensity (physics), Activity estimation, Energy expenditure, Physical therapy, Smartphone, medicine.symptom, business, Energy Metabolism, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, mobilité, Information Systems
Abstract

Physical inactivity and sedentary behaviors are on the rise worldwide and contribute to the current overweight and obesity scourge. The loss of healthy life style benchmarks and the lack of the need to move make it necessary to provide feedback about physical and sedentary activities in order to promote active ways of life. The aim of this study was to develop a specific function adapted to overweight and obese people to identify four physical activity (PA) categories and to estimate the associated total energy expenditure (TEE). This function used accelerometry data collected from a smartphone to evaluate activity intensity and length, and TEE. The performance of the proposed function was estimated according to two references (Armband® and FitmatePro®) under controlled conditions (CC) for a 1.5-h scenario, and to the Armband® device in free-living conditions (FLC) over a 12-h monitoring period. The experiments were carried out with overweight and obese volunteers: 13 in CC and 27 in FLC. The evaluation differences in time spent in each category were lower than 7% in CC and 6% in FLC, in comparison to the Armband® and FitmatePro® references. The TEE mean gap in absolute value between the function and the two references was 9.3% and 11.5% in CC, and 8.5% according to Armband® in FLC.

Published
2017

4. Weight for gestational age and metabolically healthy obesity in adults from the Haguenau cohort

Author

Mélanie Pétéra, Claire Carette, Estelle Pujos-Guillot, Joane Matta, Marie Zins, Sébastien Czernichow, Claire Levy Marchal, Blandine Comte, Julien Bertrand, ProdInra, Migration, UMS011, Institut National de la Santé et de la Recherche Médicale (INSERM), Département Nutrition, Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Unité d'épidémiologie clinique, Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, and Université Paris Descartes - Paris 5 (UPD5)

Subjects
Blood Glucose, Male, Pediatrics, Epidemiology, body-mass index, cardiovascular-disease, 030204 cardiovascular system & hematology, Cohort Studies, 0302 clinical medicine, Risk Factors, Metabolically healthy obesity, Medicine, health care economics and organizations, reproductive and urinary physiology, 2. Zero hunger, education.field_of_study, Gestational age, General Medicine, female genital diseases and pregnancy complications, Santé publique et épidémiologie, trouble métabolique, Quartile, Alimentation et Nutrition, Cohort, Infant, Small for Gestational Age, Female, France, PUBLIC HEALTH, diabète, Cohort study, Adult, medicine.medical_specialty, education, Population, NUTRITION & DIETETICS, 030209 endocrinology & metabolism, Gestational Age, 03 medical and health sciences, Young Adult, Food and Nutrition, Humans, patient obèse, Obesity, Metabolically Benign, business.industry, Research, Body Weight, Infant, Newborn, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, âge gestationnel, [SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie, Relative risk, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Insulin Resistance, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Body mass index
Abstract

Background An obesity subphenotype, named ‘metabolically healthy obese’ (MHO) has been recently defined to characterise a subgroup of obese individuals with less risk for cardiometabolic abnormalities. To date no data are available on participants born with small weight for gestational age (SGA) and the risk of metabolically unhealthy obesity (MUHO). Objective Assess the risk of MUHO in SGA versus appropriate for gestational age (AGA) adult participants. Methods 129 young obese individuals (body mass index ≥30 kg/m²) from data of an 8-year follow-up Haguenau cohort (France), were identified out of 1308 participants and were divided into 2 groups: SGA (n=72) and AGA (n=57). Metabolic characteristics were analysed and compared using unpaired t-test. The HOMA-IR index was determined for the population and divided into quartiles. Obese participants within the first 3 quartiles were considered as MHO and those in the fourth quartile as MUHO. Relative risks (RRs) and 95% CI for being MUHO in SGA versus AGA participants were computed. Results The SGA-obese group had a higher risk of MUHO versus the AGA-obese group: RR=1.27 (95% CI 1.10 to 1.6) independently of age and sex. Conclusions In case of obesity, SGA might confer a higher risk of MUHO compared with AGA.

Published
2016

5. L'invalidation génétique des protéines 4E-BP1/2 chez la souris induit l'insulino-résistance et une lipotoxicité au niveau musculaire

Author

Le Bacquer, Olivier, Combe, Kristell, Salles, Jérôme, Giraudet, Christophe, Patrac, Véronique, Montaurier, Christophe, Berry, Alexandre, Pion, Angélique, Rossignon, F, Domingues Faria, Carla, Guillet, Christelle, Boirie, Yves, Louche, Katie, Moro, Cédric, Walrand, Stephane, Unité de Nutrition Humaine (UNH), Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA), Institut des Maladies Métaboliques et Cardiovasculaires (I2MC), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche en Nutrition Humaine (CRNH). Clermont-Ferrand, FRA., Société Francophone Nutrition Clinique et Métabolisme (SFNEP). FRA., Société Française de Nutrition (SFN). FRA., Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), and ProdInra, Migration

Subjects
[SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, régime hyperlipidique, Alimentation et Nutrition, Food and Nutrition, désordre métabolique, souris, métabolisme des lipides, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS, patient obèse
Abstract

Flash Posters : Axe 1; National audience; mTOR (mammalian Target of rapamycin) est un noeud métabolique qui en réponse aux nutriments et facteurs de croissance régule de nombreux processus cellulaires. Une suractivation de mTOR est souvent observée dans les tissus de patients obèses ou les modèles animaux d’obésité et associée à l’insulinorésistance. Cependant, le rôle précis des différentes cibles de mTOR dans l’installation des désordres métaboliques est encore peu exploré. Chez la souris, la délétion des protéines 4EBP1 et 4EBP2 (cibles de mTOR) au niveau du corps entier provoque une sensibilité accrue à l’induction de l’obésité en favorisant l’adipogenèse et l’insulino-résistance. L’objectif de cette étude était de déterminer chez la souris l’impact de la délétion de 4EBP1 et 4EBP2 sur la lipotoxicité musculaire en réponse à un régime riche en graisses. Des souris sauvages (WT) et invalidées pour les protéines 4EBP1 et 2 (4EBP1/2 Double KO, DKO) ont reçu un régime standard (STD. 3,79kcal/g de régime) ou riche en graisses (HFD. 4,60kcal/g) pendant 20 semaines. A la fin de ce régime des tests ITT et ipGTT ont été réalisés pour mesurer le degré d’insulinorésistance et de tolérance au glucose. Les contenus intramusculaires en lipides, céramides et sphingomyélines ont été mesurés par chromatographie gazeuse et HPLC-MS afin de caractériser les atteintes lipidiques musculaires. L’expression des gènes impliqués dans le transport des acides gras, le métabolisme des lipides et la bêta-oxydation a été mesurée dans le muscle par PCR quantitative. L’expression d’ATGL a été mesurée par western blot et l’activité ATGL mesurée. Les résultats ont été analysés par ANOVA à 2 voies. Le régime HFD induit une prise de poids chez les souris WT et DKO, avec une prise de masse musculaire plus importante chez les souris DKO (p

Published
2016

6. A strict selection of obese patients minimizes post renal transplant complications but increases waiting time

Author

Lukaszewicz, A, Aniort, Julien, Garrouste, C., Tiple, A., Philipponnet, C., Boirie, Yves, Heng, Anne-Elisabeth, Néphrologie, Centre Hospitalier Universitaire (CHU), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, and Nutrition

Subjects
transplantation rénale, Alimentation et Nutrition, Food and Nutrition, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, patient obèse
Abstract

A strict selection of obese patients minimizes post renal transplant complications but increases waiting time

Published
2015

7. Dépense énergétique des adolescents non-obèses, obèses ou post-obèses

Author

Michel Vermorel, Lazzeri, S., Bitar, A., Christophe Montaurier, Nicole Fellmann, Jacques Coudert, Meyer, M., Yves Boirie, Unité de Recherches sur les Herbivores (URH), Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine (UNH), Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA), Laboratoire de Physiologie-Biologie du Sport, Université d'Auvergne - Clermont-Ferrand I (UdA), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Recherche Nutrition Humaine, Hôpital de l'Hôtel-Dieu de Clermont-Ferrand, Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), Unité de Nutrition Humaine - Clermont Auvergne ( UNH ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes ), Université d'Auvergne - Clermont-Ferrand I ( UdA ), and ProdInra, Archive Ouverte

Subjects
[SDV] Life Sciences [q-bio], [ SDV ] Life Sciences [q-bio], adolescent, [SDV]Life Sciences [q-bio], dépense énergétique, energy expenses, patient obèse
Abstract

Dépense énergétique des adolescents non-obèses, obèses ou post-obèses. 1. Congrès de la Société Française de Nutrition (SFN)

Published
2003

8. Energy expenditure in relation to sex, age, body composition and physical activity in obese and non-obese adolescents

Author

Vermorel, Michel, Bitar, A., Ribeyre, J., Lazzeri, S., Montaurier, Christophe, Vernet, Jean, Fellmann, Nicole, Boirie, Yves, Meyer, M., Petit, I., Taillardat, Michel, Coudert, Jean, Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Faculté de Médecine, Physiologie et Biologie du Sport, Centre Hospitalier Universitaire de Clermont-Ferrand, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Laboratoire de Physiologie-Biologie du Sport, Université d'Auvergne - Clermont-Ferrand I (UdA), Recherche Nutrition Humaine, Hôpital de l'Hôtel-Dieu de Clermont-Ferrand, Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques - Clermont Auvergne (AME2P), Faculté des Sciences du SportFaculté des Sciences du Sport, UFR STAPS-Université Clermont Auvergne (UCA), Métabolisme protéino-énergétique (UMPE), Université d'Auvergne - Clermont-Ferrand I (UdA)-Institut National de la Recherche Agronomique (INRA), Unité de Recherches sur les Herbivores (URH), Institut National de la Recherche Agronomique (INRA), CHU Clermont-Ferrand, Unité de Nutrition Humaine (UNH), Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques (AME2P), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-UFR Sciences et Techniques des Activités Physiques et Sportives - Clermont-Auvergne (UFR STAPS - UCA), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), Unité de Nutrition Humaine - Clermont Auvergne ( UNH ), Université Clermont Auvergne ( UCA ) -Institut national de la recherche agronomique [Auvergne/Rhône-Alpes] ( INRA Auvergne/Rhône-Alpes ), Université d'Auvergne - Clermont-Ferrand I ( UdA ), Laboratoire des Adaptations Métaboliques à l'Exercice en Conditions Physiologiques et Pathologiques - Clermont Auvergne ( AME2P ), Faculté des Sciences du SportFaculté des Sciences du Sport, UFR STAPS-Université Clermont Auvergne ( UCA ), UMR : UMR INRA / Univ. Clermont 1 : Métabolisme protéino-énergétique, Clermont-Ferrand, and ProdInra, Archive Ouverte

Subjects
body composition, [ SDV ] Life Sciences [q-bio], composition corporelle, [SDV]Life Sciences [q-bio], education, energy expenses, activité physique, sexe, [SDV] Life Sciences [q-bio], physical exercise, adolescent, dépense énergétique, health care economics and organizations, patient obèse
Abstract

Energy expenditure in relation to sex, age, body composition and physical activity in obese and non-obese adolescents. Réunion du Comité Scientifique International du CRNH d'Auvergne (poster)

Published
2001

9. Contribution of obese adipose tissue-derived stem cells to hepato-or breast-carcinoma inflammation, through promotion of Th17 cells and activation of IL-1b by monocytes

Author

Marwa Chehimi, Laetitia Delort, Robert, M., Hubert Vidal, Florence Caldefie-Chezet, Assia Eljaafari, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Hospices Civils de Lyon (HCL), ARC1 Sante-Region Rhone Alpes, Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), and European Association for the Study of Diabetes (EASD). GBR.

Subjects
inflammation, cellule humaine, tissu adipeux, cancer, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, adipose tissue, patient obèse
Abstract

Background and aims:As opposed with lean adipose tissues (AT), obese AT are heavily infiltrated with variety of inflammatory cells such as macrophages and Th17 cells. Obesity-mediated chronic low-grade inflammation is known to contribute to tumor progression in various cancers, including hepatic and breast cancers. Because we have previous-ly demonstrated, using co-culture experiments, that obese AT-derived stem cells (obASC) contribute to AT inflammation through promotion of Th17 cells, and activation of IL-1β-secreting monocytes, we postulated herein that such inflammatory environment could contribute to tumor progression in cancer-suffering obese patients.Materials and methods:Human ASC were isolated from AT of obese donors. Mononuclear cells (MNC) were collected from healthy blood donors. Co-cultures of obASC and MNC were activated for 48 hours with phytohemagglutinin A ( PHA), a T cell mitogen, or not. Conditionned media (CM) were collected, and added for 24h to cultures of HuH7 (hepatocarinoma cell line) or of two breast carcinoma cell lines, i.e MCF-7, or MDA-MB-231. Levels of inflammatory or angiogenic gene expression were evaluated by qRT-PCR. Expression of CXCR4 (a marker of invasiveness) was measured by flow cytometry in the HuH7 cell line.Results:CM from PHA-activated-obASC/MNC co-cultures enhanced IL-1 β ,IL-8andVEGF α mRNA expression in HuH7 cells by 1942.2, 45.7 and 6.1 -fold, respectively, as compared with no treatment. A putative effect of CM on HuH7 invasiveness was supported by a 2- and 3-fold increase in MMP-9, and CXCR4 expression, respectively. In addition, IL-1β, IL-8 and VEGF-α mRNA expression were increased by 34.3, 33.2, and 2.97 fold respectively in MCF-7 cells, and by 85.8, 52.0 , and 1.34 fold respectively, in MDA-MB-231 cells. These results indicated thus a differential sensitivity of cancer cell lines to CM from PHA-activated-ob ASC/MNC co-cultures, with a preponderant increase of IL-1 β mRNA levels in hepatocarcinoma cells versus a similar increase of IL-1β and IL-8 gene expression in breast carcinoma cells.Conclusion:Our results suggest that in cancer-suffering obese patients, interaction of obASC with AT-infiltrating immune cells contribute to the establishment of an inflammatory environment, propitious to tumor inflammation and/or tumor migration. Whether this inflammation could occur through propagation of obese AT inflammatory environment towards tumors, or through migration of ASC inside tumors and then inter-action with tumor infiltrating immune cells, remain to be explored.

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