1. Immunocytochemical detection of hMSH2 and hMLH1 expression in oral melanoma
- Author
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Lorenzo Lo Muzio, Nocini P, Md, Mignogna, Pannone G, Staibano S, Procaccini M, Rubini C, Fioroni M, Fanali S, Piattelli A, Lo Muzio, L, Nocini, P, Mignogna, MICHELE DAVIDE, Pannone, G, Staibano, Stefania, Procaccini, M, Rubinic, Fioroni, M, Fanali, S, Piattelli, A., and Rubini, C
- Subjects
Male ,Cytoplasm ,DNA Repair ,Base Pair Mismatch ,analysis ,pathology, Cytoplasm ,Proto-Oncogene Proteins ,80 and over ,Humans ,pathology/secondary ,pathology/secondary, Middle Aged, Mouth Mucosa ,Neoplasm Metastasis ,pathology, Humans, Immunohistochemistry, Lip Neoplasm ,Melanoma ,Adaptor Proteins, Signal Transducing ,Aged ,Aged, 80 and over ,Cell Nucleus ,Gingival Neoplasms ,Palatal Neoplasms ,pathology, DNA Repair, DNA-Binding Proteins, Female, Gingival Neoplasm ,pathology, Mouth Neoplasm ,Signal Transducing ,Mouth Mucosa ,Adaptor Proteins ,Nuclear Proteins ,Middle Aged ,analysis, Nuclear Proteins, Palatal Neoplasm ,Immunohistochemistry ,pathology, MutS Homolog 2 Protein, Neoplasm Metastasis, Neoplasm Protein ,Neoplasm Proteins ,DNA-Binding Proteins ,80 and over, Base Pair Mismatch, Carrier Proteins, Cell Nucleu ,MutS Homolog 2 Protein ,Adaptor Protein ,Signal Transducing, Aged, Aged ,pathology, Proto-Oncogene Protein ,Lip Neoplasms ,Melanocytes ,pathology, Melanoma ,pathology ,Female ,Mouth Neoplasms ,MutL Protein Homolog 1 ,Carrier Proteins ,pathology, Male, Melanocyte - Abstract
BACKGROUND: The DNA mismatch repair system (MMR) plays an important role in the maintenance of genomic stability. To date few studies have been performed on hMSH2 and hMLH1 expression and melanoma of the head and neck region. A study of two cases revealed no mutations of the mismatch repair genes hMSH2 and hMLH1. MATERIALS AND METHODS: To verify the possibility of implication of hMSH2 and hMLH1 alterations in melanocytic cancerogenesis, the authors examined the protein expression pattern of hMSH2 and hMLH1 by immunohistochemistry in 9 paraffin-embedded oral melanoma. RESULTS: One case (11%) showed nuclear positivity for hMSH2, 3 cases (33%) showed cytoplasmic positivity, and five cases (55%) showed no staining in the tumoral cells, even if normal squamous epithelium available in this section showed a nuclear positivity. Four cases (44%) showed no hMLH1 staining in the tumoral cells, even if normal squamous epithelium available in this section showed a nuclear positivity. Two cases (22%) showed nuclear positivity, and three cases (33%) showed cytoplasmic positivity. CONCLUSIONS: The analysis of mismatch repair genes can be a new molecular diagnostic tools for the detection of patients at high risk of developing melanoma and other neoplasia, or metastasis and recurrences.
- Published
- 2000