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Abstract Background Wohlfahrtia magnifica is an obligatory parasite that causes myiasis in several warm-blooded vertebrates. Adult females deposit the first-stage larvae directly onto wounds or natural body orifices (e.g., genitalia) of the host, from where they quickly colonize the host tissue and feed on it for development. The infestation of W. magnifica can lead to health issues, welfare concerns, and substantial economic losses. To date, little is known about the molecular mechanisms of the W. magnifica-causing myiasis. Results In this study, we collected parasitic-stage larvae of W. magnifica from wounds of naturally infested Bactrian camels, as well as pupae and adult flies reared in vitro from the wound-collected larvae, for investigating the gene expression profiles of the different developmental stages of W. magnifica, with a particular focus on examining gene families closely related to the parasitism of the wound-collected larvae. As key proteins related to the parasite-host interaction, 2049 excretory/secretory (ES) proteins were identified in W. magnifica through the integration of multiple bioinformatics approaches. Functional analysis indicates that these ES proteins are primarily involved in cuticle development, peptidase activity, immune response, and metabolic processes. The global investigation of gene expression at different developmental stages using pairwise comparisons and weighted correlation network analysis (WGCNA) showed that the upregulated genes during second-stage larvae were related to cuticle development, peptidase activity, and RNA transcription and translation; during third-stage larvae to peptidase inhibitor activity and nutrient reservoir activity; during pupae to cell and tissue morphogenesis and cell and tissue development; and during adult flies to signal perception, many of them involved in light perception, and adult behavior, e.g., feeding, mating, and locomotion. Specifically, the expression level analysis of the likely parasitism-related genes in parasitic wound-collected larvae revealed a significant upregulation of 88 peptidase genes (including 47 serine peptidase genes), 110 cuticle protein genes, and 21 heat shock protein (hsp) genes. Interestingly, the expression of 2 antimicrobial peptide (AMP) genes, including 1 defensin and 1 diptericin, was also upregulated in the parasitic larvae. Conclusions We identified ES proteins in W. magnifica and investigated their functional distribution. In addition, gene expression profiles at different developmental stages of W. magnifica were examined. Specifically, we focused on gene families closely related to parasitism of wound-collected larvae. These findings shed light on the molecular mechanisms underlying the life cycle of the myiasis-causing fly, especially during the parasitic larval stages, and provide guidance for the development of control measures against W. magnifica.

To solve the multi-objective, flexible job-shop scheduling problem, the biogeography-based optimization (BBO) algorithm can easily fall into premature convergence, local optimum and destroy the optimal solution. Furthermore, the symbiotic organisms search (SOS) strategy can be introduced, which integrates the mutualism strategy and commensalism strategy to propose a new migration operator. To address the problem that the optimal solution is easily destroyed, a parasitic natural enemy insect mechanism is introduced, and predator mutation and parasitic mutation strategies with symmetry are defined, which can be guided according to the iterative characteristics of the population. By comparing with eight multi-objective benchmark test functions with four multi-objective algorithms, the results show that the algorithm outperforms other comparative algorithms in terms of the convergence of the solution set and the uniformity of distribution. Finally, the algorithm is applied to multi-objective, flexible job-shop scheduling (FJSP) to test its practical application value, and it is shown through experiments that the algorithm is effective in solving the multi-objective FJSP problem. [ABSTRACT FROM AUTHOR]

To solve the multi-objective, flexible job-shop scheduling problem, the biogeography-based optimization (BBO) algorithm can easily fall into premature convergence, local optimum and destroy the optimal solution. Furthermore, the symbiotic organisms search (SOS) strategy can be introduced, which integrates the mutualism strategy and commensalism strategy to propose a new migration operator. To address the problem that the optimal solution is easily destroyed, a parasitic natural enemy insect mechanism is introduced, and predator mutation and parasitic mutation strategies with symmetry are defined, which can be guided according to the iterative characteristics of the population. By comparing with eight multi-objective benchmark test functions with four multi-objective algorithms, the results show that the algorithm outperforms other comparative algorithms in terms of the convergence of the solution set and the uniformity of distribution. Finally, the algorithm is applied to multi-objective, flexible job-shop scheduling (FJSP) to test its practical application value, and it is shown through experiments that the algorithm is effective in solving the multi-objective FJSP problem.

Simple Summary: Obligate brood parasite birds such as the common cuckoo (Cuculus canorus) can trick their hosts via a variety of means. Cuckoos are threats to the host nests but not to the host adults themselves. To successfully parasitize the host nests, female cuckoos have been hypothesized to distract the hosts' attention from their nests by mimicking the calls of sparrowhawks (Accipiter nisus), a predator of the hosts. We performed playback experiments in two populations of the host oriental reed warbler (Acrocephalus orientalis) that have experienced different levels of parasitic pressure. We found that female cuckoo calls evoked both populations of the hosts to leave their nests more frequently than did the calls of male cuckoos or doves that do not pose threats to the hosts. This indicated that the call of the female cuckoo functions to deceive the host and thus favors host brood parasitism. However, we propose that such a deceiving effect of the female cuckoo call is due to the rapid cadence of the call rather than sparrowhawk mimicry. The common cuckoo (Cuculus canorus) is an obligate brood parasite that has evolved a series of strategies to trick its hosts. The female cuckoo has been hypothesized to mimic the appearance and sounds of several raptors to deceive the hosts into exhibiting anti-predator behavior. Such behavior would relax the protection of the host nest and thus allow the female cuckoo to approach the host nest unopposed. Many anti-parasite strategies have been found to vary among geographical populations due to different parasitic pressures from cuckoos. However, the effect of female cuckoo calls related to different levels of parasitic pressure has not been examined. Here, we studied the effect of female cuckoo calls on the oriental reed warbler (Acrocephalus orientalis), one of the major hosts of the common cuckoo, in two geographical populations experiencing different levels of parasite pressure. Four kinds of sounds were played back to the hosts: the calls from female common cuckoos, male common cuckoos, sparrowhawks (Accipiter nisus), and oriental turtle doves (Streptopelia orientalis). The results showed that the female cuckoo calls induced the hosts to leave their nests more frequently than the male cuckoo or dove calls in both populations, and two populations of the hosts reacted similarly to the female cuckoo calls, implying that the function of female cuckoo calls would not be affected by the difference in parasitism rate. This study indicates that female cuckoo calls function to distract the hosts' attention from protecting their nests. However, we propose that such a deception by the female cuckoo call may not be due to the mimicry of sparrowhawk calls, but rather that the rapid cadence of the call that causes a sense of anxiety in the hosts. [ABSTRACT FROM AUTHOR]

The common cuckoo (Cuculus canorus) is an obligate brood parasite that has evolved a series of strategies to trick its hosts. The female cuckoo has been hypothesized to mimic the appearance and sounds of several raptors to deceive the hosts into exhibiting anti-predator behavior. Such behavior would relax the protection of the host nest and thus allow the female cuckoo to approach the host nest unopposed. Many anti-parasite strategies have been found to vary among geographical populations due to different parasitic pressures from cuckoos. However, the effect of female cuckoo calls related to different levels of parasitic pressure has not been examined. Here, we studied the effect of female cuckoo calls on the oriental reed warbler (Acrocephalus orientalis), one of the major hosts of the common cuckoo, in two geographical populations experiencing different levels of parasite pressure. Four kinds of sounds were played back to the hosts: the calls from female common cuckoos, male common cuckoos, sparrowhawks (Accipiter nisus), and oriental turtle doves (Streptopelia orientalis). The results showed that the female cuckoo calls induced the hosts to leave their nests more frequently than the male cuckoo or dove calls in both populations, and two populations of the hosts reacted similarly to the female cuckoo calls, implying that the function of female cuckoo calls would not be affected by the difference in parasitism rate. This study indicates that female cuckoo calls function to distract the hosts’ attention from protecting their nests. However, we propose that such a deception by the female cuckoo call may not be due to the mimicry of sparrowhawk calls, but rather that the rapid cadence of the call that causes a sense of anxiety in the hosts.

In host-parasitoid interactions, antagonistic relationship drives parasitoids to vary in virulence in facing different hosts, which makes these systems excellent models for stress-induced evolutionary studies. Venom compositions varied between two strains of Tetrastichus brontispae, Tb-Bl and Tb-On. Tb-Bl targets Brontispa longissima pupae as hosts, and Tb-On is a sub-population of Tb-Bl, which has been experimentally adapted to a new host, Octodonta nipae. Aiming to examine variation in parasitoid virulence of the two strains toward two hosts, we used reciprocal injection experiments to compare effect of venom/ovarian fluids from the two strains on cytotoxicity, inhibition of immunity and fat body lysis of the two hosts. We found that Tb-Onvenom was more virulent towards plasmatocyte spreading, granulocyte function and phenoloxidase activity than Tb-Blvenom. Tb-Blovary was able to suppress encapsulation and phagocytosis in both hosts; however, Tb-Onovary inhibition targeted only B. longissima. Our data suggest that the venom undergoes rapid evolution when facing different hosts, and that the wasp has good evolutionary plasticity. [ABSTRACT FROM AUTHOR]