1. Effect of ampicillin on formed biofilm of Escherichia coli
- Author
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Nagode, Anja and Podgornik, Aleš
- Subjects
coil bioreactor ,resistance to antibiotics ,E. coli ,cevni bioreaktor ,biofilm ,odpornost na antibiotike - Abstract
Biofilmi so združbe bakterijskih celic, ki živijo pritrjene na podlago v vlažnih okoljih. Znotraj združbe prihaja tako do sodelovanja kot do izkoriščanja med bakterijami. Celice so med seboj povezane z zunajceličnim matriksom, ki omogoča komunikacijo, prav tako pa pripomore k zaščiti pred zunanjimi vplivi, imunskim sistemom in protimikrobnimi sredstvi. Celice imajo tudi upočasnjen metabolizem in spremenjeno izražanje genov, kar le še pripomore k težavni odstranitvi okužb z biofilmi. Ker le te predstavljajo velik problem v zdravstvu, je bistvenega pomena poznavanje odziva biofilmov na terapije z antibiotiki. V diplomskem delu smo vzpostavili bioreaktorski sistem, v katerem smo različno dolgo tvorili biofilme bakterije Escherichia coli ter jih spirali z dvema različnima koncentracijama ampicilina v SM pufru. Tekom daljšega časa spiranja se je dovzetnost bakterij za antibiotik nižala. Višja koncentracija ampicilina je imela večji vpliv tako na biofilme kot na planktonske celice. Tudi čas tvorjenja biofilma je imel vpliv na kočno koncentracijo celic, saj so bile pri daljšem nalaganju pri vseh vzorčenjih gostote celic višje. Biofilms are communities of bacterial cells that live attached to a surface in moist environments. Within a community, both cooperation and exploitation can occur. Cells are connected to each other with extracelullar matrix that enables communication and at the same time protects cells against external influences, the immune system and antimicrobial agents. Cells in biofilms have slower metabolism and altered gene expression, which contribute to the difficult removal of biofilm infections. The latter represent a major problem in healthcare, so it is essential to understand the responses of biofilms to antibiotic therapy. In our thesis, we formed Escherichia coli biofilms in coiled bioreactor and tested how different loading times as well as different concentrations of antibiotic ampicilin in SM buffer affect biofilms and planktonic cells. During the rinsing of biofilms, the susceptibility to the antibiotic decreased. We showed that a higher concentration of ampicilin has a greater effect on both biofilms and planktonic cells. Longer loading time had an impact on the final concentration of cells, as the cell densities were higher with longer loading period.
- Published
- 2023