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Molecular studies on odorant receptors (ORs), odorant‐binding proteins (OBPs) and the functioning of the receptor and pheromone signal transduction in fruit fly Bactrocera species have expanded exponentially during the past few decades. OBPs contribute to the sensing of the olfactory system (OS) via the transduction of odorants through the sensillum lymph. However, ORs, a family of G‐protein‐coupled receptors in Bactrocera and various other species, exhibit heightened responsiveness to multiple chemical odours such as hormones, sensory stimuli and neurotransmitters. The apparent mechanism involves a combinatorial code encompassing both peripheral and antennal lobe processing, facilitating the reception of sexual pheromones and environmental cues. The OS is specifically designed to recognize and process information from volatile chemical signals, and these chemical signals play an important function in various flies. Insects rely on these chemicals to navigate and comprehend their surroundings. A mature insect OS is composed of two pairs of sensillae‐covered palps, antennae and two primary pairs of olfactory appendages on the anterior head. It has been shown that chemosensory gene families contribute in odour perception. These include various neuroreceptor families, such as OBPs, chemosensory proteins and sensory neuron membrane proteins. Additionally, there are three divergent chemoreceptors, namely ORs, ionotropic receptors and gustatory receptors. Methods based on systematic biology, molecular biology and bioinformatics tools have rapidly emerged to investigate the insect communication systems and provide new insights for the management of many agricultural pest. Several aromatic compounds, including semiochemicals and pheromones, have been employed to defend crops and animals from destructive fruit flies and other invasive and frugivorous species. To promote the expansion of the cropping system, the utilization of phytochemical lures can be convenient for sustainable agriculture production and enhance food security. Hence, this review examined the state of the art in chemical communication of insects with a focus on fruit fly pest species to identify OS and their semiochemical receptors, protein receptors and chemosensory receptors (CSRs), as well as their practical applications for biological control and integrated pest management are highlighted. [ABSTRACT FROM AUTHOR]

The spectrum resource is always a critical issue for wireless communications since it directly impacts the data rate and capacity. However, the problem of spectrum resource scarcity always exists. Moreover, spectrum resource scarcity becomes more severe as new communication technologies and wireless applications sprout. Noncontiguous orthogonal frequency division multiplexing (NC-OFDM) is a multicarrier method for bandwidth utilization. Unfortunately, this system has two fatal defects: high peak-to-average power ratio (PAPR) and considerable out-of-band power (OBP), which are detrimental to the system's performance. To solve these two problems, we propose a convex optimization-based method for joint PAPR and OBP reduction in NC-OFDM Systems. The strategy is to permit the secondary user to utilize the unoccupied spectrum of the primary user with dynamic spectrum sharing (DSS) based on a cognitive radio network (CRN). To this end, a flexible system operating over noncontiguous bands and DSS scenarios is necessary. The simulation results have shown that our method could effectively improve the overall performance and outperform other schemes, i.e., projections onto convex sets (POCS) and alternating projections onto convex and non-convex sets (APOCNCS), without harming the transmission of the primary system. The collaboration between secondary and primary systems is viable with the proposed method.

In order to cater long distance optical network units (subscribers), optical networks with prolonged reach are needed. In this study, an optical back propagation (OBP) dual polarization (DP)-128 QAM-based system is proposed to cope with nonlinear impairments in wavelength division multiplexing (WDM) systems. In pre, post and symmetrical configurations, the OBP module consisting of the optical phase conjugator (OPC), Raman fiber amplifier (RFA) and erbium doped fiber amplifier (EDFAs) is examined. Using dispersion compensation fiber (DCF) as RFA with dual directional pumping, ideal OBP conditions are simulated. DP-128 quadrature amplitude modulation (QAM) system is proposed with symmetrical OBP and compared with single channel digital back propagation (DBP), wideband DBP, pre OBP-forward pumping, pre OBP with backward pumping, pre OBP with dual directional pumping, post OBP with dual directional pumping. It is observed that symmetrical OBP configuration with dual direction pumping provide enhanced results and successfully cover 6500 km at bit error rate (BER) 10−3. [ABSTRACT FROM AUTHOR]

This review paper scrutinizes the staging of dense wavelength division multiplexing soliton system in return to zero and non-return to zero format. In-line optical phase conjugator (OPC) is used to depreciate the power of four wave mixing (FWM). Destructive interference between both the halves of in-line OPC plummets the FWM power sturdily. This paper concludes with the indication that return-to-zero with OPC gives out better performance with FWM suppression. [ABSTRACT FROM AUTHOR]

Sea level rise has important implications for coastal regions. In this study, seasonal and interannual variations of sea level in the Bohai Sea and Yellow Sea (BYS) from 1993 to 2017 are investigated using the reanalysis product Estimating the Circulation and Climate of the Ocean (ECCO). Sea level is comprised of two major components: steric sea level and ocean bottom pressure (OBP). On seasonal time scales, steric sea level and OBP have considerable contributions to the sea level variations in the BYS. The sea level in the deep water is dominated by steric sea level, while OBP controls the coastal sea level. Diagnostic analysis further suggests that the steric change is owing to temperature change. On interannual timescales, the contribution of steric effect is indiscernible, and OBP dominates the variability of sea level in the BYS. The seasonal and interannual variability of OBP is forced by wind-induced Ekman transport, and is closely related to the North Pacific Oscillation (NPO) during wintertime. Moreover, Kuroshio modulates the interannual variability of sea level in the BYS via controlling water volume transport from the Pacific Ocean to the BYS. This study sheds light on the mechanism of sea level low-frequency variability in the BYS, and complements the dynamic processes along the coast. [ABSTRACT FROM AUTHOR]

Simple Summary: The Asian longhorned beetle (ALB), Anoplophora glabripennis Motschulsky, is a destructive pest in its native habitat and one of the most serious invasive alien species in North America and Europe, causing substantial economic and ecological losses. In order to explore effective monitoring and management strategies, we summarize and create a comprehensive list of host plants, including 209 species (cultivars) that have been damaged by ALBs. Thus far, 143 olfactory protein genes have been found in ALBs. Host kairomones were preferentially bound to ALB recombinant odorant-binding proteins (OBPs), but the function of most OBPs is still unclear. Microbial communities may help ALBs degrade host plants. We analyzed the trapping effect of combined host kairomones and sex pheromones and found that trapping numbers are limited in the field. Therefore, we discussed host plant location behavioral processes from new perspectives and found that multiple cues are used to locate and recognize host plants. Overall, we suggest that further research should contribute to understanding the host resistance mechanism, microbial community influence mechanism, and visual cue recognition mechanism of host plants. This research may provide effective monitoring and management strategies for ALBs. The Asian longhorn beetle (ALB), Anoplophora glabripennis Motschulsky, is a polyphagous xylophage with dozens of reported host tree species. However, the mechanisms by which individuals locate and recognize host plants are still unknown. We summarize the current knowledge of the host plant list, host kairomones, odorant-binding proteins (OBPs) and microbial symbionts of this beetle and their practical applications, and finally discuss the host localization and recognition mechanisms. A total of 209 species (or cultivars) were reported as ALB host plants, including 101 species of higher sensitivity; host kairomones were preferentially bound to ALB recombinant OBPs, including cis-3-hexen-1-ol, δ-3-carene, nonanal, linalool, and β-caryophyllene. In addition, microbial symbionts may help ALB degrade their host. Complementarity of tree species with different levels of resistance may reduce damage, but trapping effectiveness for adults was limited using a combination of host kairomones and sex pheromones in the field. Therefore, we discuss host location behavior from a new perspective and show that multiple cues are used by ALB to locate and recognize host plants. Further research into host resistance mechanisms and visual signal recognition, and the interaction of sex pheromone synthesis, symbiont microbiota, and host plants may help reveal the host recognition mechanisms of ALBs. [ABSTRACT FROM AUTHOR]

The Asian longhorn beetle (ALB), Anoplophora glabripennis Motschulsky, is a polyphagous xylophage with dozens of reported host tree species. However, the mechanisms by which individuals locate and recognize host plants are still unknown. We summarize the current knowledge of the host plant list, host kairomones, odorant-binding proteins (OBPs) and microbial symbionts of this beetle and their practical applications, and finally discuss the host localization and recognition mechanisms. A total of 209 species (or cultivars) were reported as ALB host plants, including 101 species of higher sensitivity; host kairomones were preferentially bound to ALB recombinant OBPs, including cis-3-hexen-1-ol, δ-3-carene, nonanal, linalool, and β-caryophyllene. In addition, microbial symbionts may help ALB degrade their host. Complementarity of tree species with different levels of resistance may reduce damage, but trapping effectiveness for adults was limited using a combination of host kairomones and sex pheromones in the field. Therefore, we discuss host location behavior from a new perspective and show that multiple cues are used by ALB to locate and recognize host plants. Further research into host resistance mechanisms and visual signal recognition, and the interaction of sex pheromone synthesis, symbiont microbiota, and host plants may help reveal the host recognition mechanisms of ALBs.

Noncontiguous orthogonal frequency division multiplexing (NC‐OFDM)‐based cognitive radio (CR) systems achieve highly efficient spectrum utilization by transmitting unlicensed users' data on subcarriers of licensed users’ data when they are free. However, there are two disadvantages to the NC‐OFDM system: out‐of‐band power (OBP) and a high peak‐to‐average power ratio (PAPR). OBP arises due to side lobes of an NC‐OFDM signal in the frequency domain, and it interferes with the spectrum for unlicensed users. A high PAPR occurs due to the inverse fast Fourier transform (IFFT) block used in an NC‐OFDM system, and it induces nonlinear effects in power amplifiers. In this study, we propose an algorithm called “Alternative Projections onto Convex and Non‐Convex Sets” that reduces the OBP and PAPR simultaneously. The alternate projections are performed onto these sets to form an iteration, and it converges to the specified limits of in‐band‐power, peak amplitude, and OBP. Furthermore, simulations show that the bit error rate performance is not degraded while reducing OBP and PAPR.

Abstract Background Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms, especially for masson pine. So far, less literature was reported on this pest. In the present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time. Results Totally, 26 odorant-binding protein (OBP) genes, 19 chemosensory protein (CSP) genes, 55 odorant receptor (OR) genes and 20 ionotropic receptor (IR) genes were identified from the C. pinicolalis antennae transcriptome and amino sequences were annotated against homologs of C. punctiferalis. The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis. Furthermore, the reference genes were selected, and we recommended the phosphate dehydrogenase gene (GAPDH) or ribosomal protein 49 gene (RP49) to verify the target gene expression during larval development stages and RP49 or ribosomal protein L13 gene (RPL13) for adult tissues. Conclusions Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis, which might be supportive for pest management studies in future.

Odorant binding proteins (OBPs) are a group of soluble proteins functioning as odorant carriers in insect antennae, mouth parts and other chemosensory organs. However, multiple insect OBPs have been detected in other tissues and various functions have been proposed. Therefore, a detailed expression profile including stages, tissues and sexes where OBPs are expressed will assist in building the links to their potential functions, enhancing the functional studies of insect OBPs. Here, we identified 39 putative OBP genes from its genome and transcriptome sequences of diamondback moth (DBM), Plutella xylostella. The expression patterns of identified PxylOBPs were further investigated from eggs, larvae, pupae, virgin adults, mated adults, larval midgut, larval heads, adult antennae, adult heads and adult tarsi. Moreover, P. xylostella larvae and adults with and without host plants for 5 h were utilized to study the interactions between OBP expression and host plants. The results showed that most PxylOBPs were highly expressed in male and female adult antennae. The expression levels of certain PxyOBPs could be regulated by mating activities and feeding host plants. This study advances our knowledge of P. xylostella OBPs, which may help develop new strategies for more environmentally sustainable management of P. xylostella. [ABSTRACT FROM AUTHOR]

Penelitian ini bertujuan untuk menganalisis jaringan informasi dan komunikasi organisasi dagang terbesar di abad 17 dan 18, Vereenigde Oost-Indische Compagnie (VOC), khususnya organisasi VOC di Sulawesi (Makasar). Arsip yang diteliti adalah khazanah arsip VOC yang terdapat di Nationaal Archief (NA) Belanda, yaitu arsip Overgekomen Brieven en Papieren (OBP) nomor 2381. Metode yang digunakan adalah deskriptif analisis dengan menggunakan sumber primer arsip OBP dan sumber sekunder literatur yang berkaitan dengan jaringan dan VOC. Hasil penelitian ini menunjukan bahwa arsip OBP memiliki struktur yang dapat menggambarkan sistem komunikasi VOC, dan topik yang paling banyak didiskusikan dalam korespondensi antara organisasi VOC di Makasar dengan pos-pos terluar adalah mengenai bajak laut dan perang.

Noncontiguous orthogonal frequency division multiplexing (NC‐OFDM)‐based cognitive radio (CR) systems achieve highly efficient spectrum utilization by transmitting unlicensed users' data on subcarriers of licensed users' data when they are free. However, there are two disadvantages to the NC‐OFDM system: out‐of‐band power (OBP) and a high peak‐to‐average power ratio (PAPR). OBP arises due to side lobes of an NC‐OFDM signal in the frequency domain, and it interferes with the spectrum for unlicensed users. A high PAPR occurs due to the inverse fast Fourier transform (IFFT) block used in an NC‐OFDM system, and it induces nonlinear effects in power amplifiers. In this study, we propose an algorithm called "Alternative Projections onto Convex and Non‐Convex Sets" that reduces the OBP and PAPR simultaneously. The alternate projections are performed onto these sets to form an iteration, and it converges to the specified limits of in‐band‐power, peak amplitude, and OBP. Furthermore, simulations show that the bit error rate performance is not degraded while reducing OBP and PAPR. [ABSTRACT FROM AUTHOR]

• OBP1/OBP2 complex formed a large binding pocket. • Interacted with the ligands by hydrogen bonds and hydrophobic interactions. • Thirteen mutants generated by site-directed mutagenesis. • Hydrogen bonds played a key role in response to odors in H. oblita. Insect odorant-binding proteins (OBPs) play key roles in transport odors to receptors and contribute to insect survival. The cooperative interaction of HoblOBP1 and HoblOBP2 in Holotrichia oblita Faldermann (Coleoptera: Melolonthidae) could increase their binding capacity for ligands. In present study, molecular docking results showed that OBP1/OBP2 complex formed a large binding pocket and interacted with the ligands by hydrogen bonds and hydrophobic interactions. Then, nine amino acids for single site mutations, three paired for double sites, and negative control were mutated into alanine successfully by site-directed mutagenesis. Finally, fluorescence binding assays of these mutants showed that breaking one or two pairs of hydrogen bonds between HoblOBP1 and HoblOBP2 or formed with the ligands significantly decrease the binding affinity with the ligands. However, hydrophobic site mutants still showed slight binding affinity to the ligands. Therefore, the three pairs of hydrogen bonds involved in heterodimer formation and the five hydrogen bonding sites in binding pocket played a key role in response to odors in H. oblita. Our findings may promote further understanding of the mechanisms underlying OBP dimer formation and the role of OBP dimers in odor perception and discrimination. [ABSTRACT FROM AUTHOR]

Background: Conogethes pinicolalis (Lepidoptera: Crambidae), is similar to Conogethes punctiferalis (yellow peach moth) and its host plant is gymnosperms, especially for masson pine. So far, less literature was reported on this pest. In the present study, we sequenced and characterized the antennal transcriptomes of male and female C. pinicolalis for the first time. Results: Totally, 26 odorant-binding protein (OBP) genes, 19 chemosensory protein (CSP) genes, 55 odorant receptor (OR) genes and 20 ionotropic receptor (IR) genes were identified from the C. pinicolalis antennae transcriptome and amino sequences were annotated against homologs of C. punctiferalis. The neighbor-joining tree indicated that the amino acid sequence of olfactory related genes is highly homologous with C. punctiferalis. Furthermore, the reference genes were selected, and we recommended the phosphate dehydrogenase gene (GAPDH) or ribosomal protein 49 gene (RP49) to verify the target gene expression during larval development stages and RP49 or ribosomal protein L13 gene (RPL13) for adult tissues. Conclusions: Our study provides a starting point on the molecular level characterization between C. pinicolalis and C. punctiferalis, which might be supportive for pest management studies in future. [ABSTRACT FROM AUTHOR]

Abstract Pharmaceutical residues are polluting the surface water environments worldwide. Sewage and wastewater treatment, therefore, needs to be improved in order to remove pharmaceutical residues from the effluent. One such treatment improvement is effluent ozonation. Even though ozonation has proven to be very efficient in reducing pharmaceutical parent compound concentrations in wastewater effluents, much remains unclear regarding potentially toxic ozonation by-product (OBP) formation. In this study, we sought to elucidate the aquatic toxicity of ozonated pharmaceuticals in zebrafish (Danio rerio) embryos in a static 144 h post fertilization (hpf) fish embryotoxicity (ZFET) assay. Three pharmaceuticals commonly detected in wastewater effluents, i.e. carbamazepine, diclofenac, and oxazepam, were selected for testing. Toxicity was assessed before and after 1 min ozonation (0.053 mg L−1 peak O 3 concentration) and 10 min ozonation (0.147 mg L−1 peak O 3 concentration). Chemical analysis showed that carbamazepine and diclofenac were largely removed by ozone (90 ± 11% and 97 ± 3.8%), whereas oxazepam was removed to a lesser extent (19 ± 5.7%). The ZFET assay revealed diverging toxicities. Diclofenac embryotoxicity decreased with increasing ozonation. Oxazepam did not cause embryotoxicity in the ZFET assay either pre- or post ozonation, but larvae swimming activity was affected at 144 hpf. Carbamazepine embryotoxicity, on the other hand, increased with increasing ozonation. Chemical analysis showed the formation of two OBPs (carbamazepine-10,11-epoxide and 10,11-dihydrocarbamazepine), possibly explaining the increased embryotoxicity. The results of this study highlight the importance of new chemical and toxicological knowledge regarding the formation of OBPs in post-ozonated effluents. Highlights • First time evaluation of Danio rerio embryo toxicity of ozonated pharmaceuticals. • Ozonation of carbamazepine increased the toxicity in all tested endpoints. • Three analyzed carbamazepine ozonation by-products were formed. • Diclofenac toxicity was completely eliminated by ozonation. • Ozonated oxazepam exposure elicited swimming behavioral changes in 6 day old larvae. [ABSTRACT FROM AUTHOR]

Mammalian olfaction depends on chemosensory neurons of the main olfactory epithelia (MOE), and/or of the accessory olfactory epithelia in the vomeronasal organ (VNO). Thus, we have generated the VNO and MOE transcriptomes and the nasal cavity proteome of the house mouse, Mus musculus musculus. Both transcriptomes had low levels of sexual dimorphisms, while the soluble proteome of the nasal cavity revealed high levels of sexual dimorphism similar to that previously reported in tears and saliva. Due to low levels of sexual dimorphism in the olfactory receptors in MOE and VNO, the sex-specific sensing seems less likely to be dependent on receptor repertoires. However, olfaction may also depend on a continuous removal of background compounds from the sites of detection. Odorant binding proteins (OBPs) are thought to be involved in this process and in our study Obp transcripts were most expressed along other lipocalins (e.g., Lcn13, Lcn14) and antimicrobial proteins. At the level of proteome, OBPs were highly abundant with only few being sexually dimorphic. We have, however, detected the major urinary proteins MUP4 and MUP5 in males and females and the male-biased central/group-B MUPs that were thought to be abundant mainly in the urine. The exocrine gland-secreted peptides ESP1 and ESP22 were male-biased but not male-specific in the nose. For the first time, we demonstrate that the expression of nasal lipocalins correlates with antimicrobial proteins thus suggesting that their individual variation may be linked to evolvable mechanisms that regulate natural microbiota and pathogens that regularly enter the body along the ‘eyes-nose-oral cavity’ axis.

Olfaction, a primal and effective sense, profoundly impacts our emotions and instincts. This sensory system plays a crucial role in detecting volatile organic compounds (VOCs) and realizing the chemical environment. Animals possess superior olfactory systems compared to humans. Thus, taking inspiration from nature, artificial olfaction aims to achieve a similar level of excellence in VOC detection. In this study, we present the development of an artificial olfaction sensor utilizing a nanostructured bio-field-effect transistor (bio-FET) based on transition metal dichalcogenides and the Drosophila odor-binding protein LUSH. To create an effective sensing platform, we prepared a hexagonal nanoporous structure of molybdenum disulfide (MoS 2 ) using block copolymer lithography and selective etching techniques. This structure provides plenty of active sites for the integration of the LUSH protein, enabling enhanced binding with ethanol (EtOH) for detection purposes. The coupling of the biomolecule with EtOH influences the bio-FETs potential, which generates indicative electrical signals. By mimicking the sniffing techniques observed in Drosophila , these bio-FETs exhibit an impressive limit of detection of 10 -6 % for EtOH, with high selectivity, sensitivity, and detection ability even in realistic environments. This bioelectric sensor demonstrates substantial potential in the field of artificial olfaction, offering advancements in VOC detection.

Mammalian olfaction depends on chemosensory neurons of the main olfactory epithelia (MOE), and/or of the accessory olfactory epithelia in the vomeronasal organ (VNO). Thus, we have generated the VNO and MOE transcriptomes and the nasal cavity proteome of the house mouse, Mus musculus musculus. Both transcriptomes had low levels of sexual dimorphisms, while the soluble proteome of the nasal cavity revealed high levels of sexual dimorphism similar to that previously reported in tears and saliva. Due to low levels of sexual dimorphism in the olfactory receptors in MOE and VNO, the sexspecific sensing seems less likely to be dependent on receptor repertoires. However, olfaction may also depend on a continuous removal of background compounds from the sites of detection. Odorant binding proteins (OBPs) are thought to be involved in this process and in our study Obp transcripts were most expressed along other lipocalins (e.g., Lcn13, Lcn14) and antimicrobial proteins. At the level of proteome, OBPs were highly abundant with only few being sexually dimorphic. We have, however, detected the major urinary proteins MUP4 and MUP5 in males and females and the male-biased central/group-B MUPs that were thought to be abundant mainly in the urine. The exocrine gland-secreted peptides ESP1 and ESP22 were male-biased but not male-specific in the nose. For the first time, we demonstrate that the expression of nasal lipocalins correlates with antimicrobial proteins thus suggesting that their individual variation may be linked to evolvable mechanisms that regulate natural microbiota and pathogens that regularly enter the body along the 'eyes-nose-oral cavity' axis. [ABSTRACT FROM AUTHOR]

Insect Odorant Binding Proteins (OBPs) constitute important components of their olfactory apparatus, as they are essential for odor recognition. OBPs undergo conformational changes upon pH change, altering their interactions with odorants. Moreover, they can form heterodimers with novel binding characteristics. Anopheles gambiae OBP1 and OBP4 were found capable of forming heterodimers possibly involved in the specific perception of the attractant indole. In order to understand how these OBPs interact in the presence of indole and to investigate the likelihood of a pH-dependent heterodimerization mechanism, the crystal structures of OBP4 at pH 4.6 and 8.5 were determined. Structural comparison to each other and with the OBP4-indole complex (3Q8I, pH 6.85) revealed a flexible N-terminus and conformational changes in the α4-loop-α5 region at acidic pH. Fluorescence competition assays showed a weak binding of indole to OBP4 that becomes further impaired at acidic pH. Additional Molecular Dynamic and Differential Scanning Calorimetry studies displayed that the influence of pH on OBP4 stability is significant compared to the modest effect of indole. Furthermore, OBP1-OBP4 heterodimeric models were generated at pH 4.5, 6.5, and 8.5, and compared concerning their interface energy and cross-correlated motions in the absence and presence of indole. The results indicate that the increase in pH may induce the stabilization of OBP4 by increasing its helicity, thereby enabling indole binding at neutral pH that further stabilizes the protein and possibly promotes the creation of a binding site for OBP1. A decrease in interface stability and loss of correlated motions upon transition to acidic pH may provoke the heterodimeric dissociation allowing indole release. Finally, we propose a potential OBP1-OBP4 heterodimer formation/disruption mechanism induced by pH change and indole binding. [Display omitted] • A remarkable destabilization of the N-terminus and α4-loop-α5 region of OBP4 was observed at acidic pH. • Increase of pH from acidic to neutral significantly stabilizes the OBP4 structure, while the stabilizing effect of indole is modest. • Binding studies showed a weak binding of indole to OBP4 that becomes further impaired at acidic pH. • pH and indole binding to OBP4, together, promote the formation of a functional OBP1-OBP4 heterodimer interface. • A decrease in interface stability and loss of correlated motions upon transition to acidic pH may provoke the heterodimeric dissociation allowing indole release. [ABSTRACT FROM AUTHOR]

Personal protection measures against the mosquitoes like the use of repellents constitute valuable tools in the effort to prevent the transmission of vector-borne diseases. Therefore, the discovery of novel repellent molecules which will be effective at lower concentrations and provide a longer duration of protection remains an urgent need. Mosquito Odorant-Binding Proteins (OBPs) involved in the initial steps of the olfactory signal transduction cascade have been recognized not only as passive carriers of odors and pheromones but also as the first molecular filter to discriminate semiochemicals, hence serving as molecular targets for the design of novel pest control agents. Among the three-dimensional structures of mosquito OBPs solved in the last decades, the OBP1 complexes with known repellents have been widely used as reference structures in docking analysis and molecular dynamics simulation studies for the structure-based discovery of new molecules with repellent activity. Herein, ten compounds known to be active against mosquitoes and/or displaying a binding affinity for Anopheles gambiae AgamOBP1 were used as queries in an in silico screening of over 96 million chemical samples in order to detect molecules with structural similarity. Further filtering of the acquired hits on the basis of toxicity, vapor pressure, and commercial availability resulted in 120 unique molecules that were subjected to molecular docking studies against OBP1. For seventeen potential OBP1-binders, the free energy of binding (FEB) and mode of interaction with the protein were further estimated by molecular docking simulations leading to the selection of eight molecules exhibiting the highest similarity with their parental compounds and favorable energy values. The in vitro determination of their binding affinity to AgamOBP1 and the evaluation of their repellent activity against female Aedes albopictus mosquitoes revealed that our combined ligand similarity screening and OBP1 structure-based molecular docking successfully detected three molecules with enhanced repellent properties. A novel DEET-like repellent with lower volatility (8.55 × 10−4 mmHg) but a higher binding affinity for OBP1 than DEET (1.35 × 10−3 mmHg). A highly active repellent molecule that is predicted to bind to the secondary Icaridin (sIC)-binding site of OBP1 with higher affinity than to the DEET-site and, therefore, represents a new scaffold to be exploited for the discovery of binders targeting multiple OBP sites. Finally, a third potent repellent exhibiting a high degree of volatility was found to be a strong DEET-site binder of OBP1 that could be used in slow-release formulations. [Display omitted] • A combined OBP1-based in silico and in vitro screening protocol led to three potent repellents and a potential attractant. • Low volatility and toxicity, commercial availability and low cost should be considered when discovering novel repellents. • A low-volatile compound with 2D-fingerprint similarity to Chrysanthenyl acetate exhibits DEET-like activity in bioassays. • Ligands with high in vitro affinity and specificity for female antennal OBPs are more likely to display strong repellence. • The discovery of compounds that bind in multiple protein sites will increase the repertoire of "olfactophore" features. [ABSTRACT FROM AUTHOR]

Reception of odorants is essential in insects’ life since the chemical signals in the environment (=semiochemicals) convey information about availability of hosts for a blood meal, mates for reproduction, sites for oviposition and other relevant information for fitness in the environment. Once they reach the antennae, these semiochemicals bind to odorant-binding proteins and are transported through the sensillar lymph until reach the odorant receptors. Such perireceptor events, particularly the interactions with transport proteins, are the liaison between the external environment and the entire neuroethological system and, therefore, a potential target to disrupt insect chemical communication. In this study, a proteomic profile of female and male antennae of Rhodnius prolixus, a vector of Chagas disease, was obtained in an attempt to unravel the entire repertoire of olfactory proteins involved in perireceptor events. Using shotgun proteomics and two-dimensional gel electrophoresis approaches followed by nano liquid chromatography coupled with tandem LTQ Velos Orbitrap mass spectrometry, we have identified 581 unique proteins. Putative olfactory proteins, including 17 odorant binding proteins, 6 chemosensory proteins, 2 odorant receptors, 3 transient receptor channels and 1 gustatory receptor were identified. Proteins involved in general cellular functions such as generation of precursor metabolites, energy generation and catabolism were expressed at high levels. Additionally, proteins that take part in signal transduction, ion binding, and stress response, kinase and oxidoreductase activity were frequent in antennae from both sexes. This proteome strategy unraveled for the first time the complex nature of perireceptor and other olfactory events that occur in R. prolixus antennae, including evidence for phosphorylation of odorant-binding and chemosensory proteins. These findings not only increase our understanding of the olfactory process in triatomine species, but also identify potential molecular targets to be explored for population control of such insect vectors. [ABSTRACT FROM AUTHOR]

Among several proteins participating in the olfactory perception process of insects, Odorant Binding Proteins (OBPs) are today considered valid targets for the discovery of compounds that interfere with their host-detection behavior. The 3D structures of Anopheles gambiae mosquito AgamOBP1 in complex with the known synthetic repellents DEET and Icaridin have provided valuable information on the structural characteristics that govern their selective binding. However, no structure of a plant-derived repellent bound to an OBP has been available until now. Herein, we present the novel three-dimensional crystal structures of AgamOBP5 in complex with two natural phenolic monoterpenoid repellents, Carvacrol and Thymol, and the MPD molecule. Structural analysis revealed that both monoterpenoids occupy a binding site (Site-1) by adopting two alternative conformations. An additional Carvacrol was also bound to a secondary site (Site-2) near the central cavity entrance. A protein-ligand hydrogen-bond network supplemented by van der Waals interactions spans the entire binding cavity, bridging α4, α6, and α3 helices and stabilizing the overall structure. Fluorescence competition and Differential Scanning Calorimetry experiments verified the presence of two binding sites and the stabilization effect on AgamOBP5. While Carvacrol and Thymol bind to Site-1 with equal affinity in the submicromolar range, they exhibit a significantly lower and distinct binding capacity for Site-2 with Kd's of ~7 μΜ and ~18 μΜ, respectively. Finally, a comparison of AgamOBP5 complexes with the AgamOBP4-Indole structure revealed that variations of ligand-interacting aminoacids such as A109T, I72M, A112L, and A105T cause two structurally similar and homologous proteins to display different binding specificities. [Display omitted] • The isomeric monoterpenoid phenols Carvacrol and Thymol were found to bind to AgamOBP5 with enhanced affinity. • The interaction of AgamOBP5 with both Carvacrol and Thymol induces considerable protein stabilization. • Structural analysis of AgamOBP5-ligand complexes revealed two binding sites with distinct binding capacities. • Carvacrol and Thymol occur in two alternative conformations in the primary high-affinity binding site. • Variations of AgamOBP5 and AgamOBP4 ligand-interacting aminoacids might account for their different binding specificities. • AgamOBP5 constitutes a novel molecular target for OBP-structure-based approaches toward developing efficient behavior disruptors to control insects. [ABSTRACT FROM AUTHOR]

Los proyectos son el medio estratégico por el cual las organizaciones implementan sus iniciativas de mejora; sin embargo, no todas son capaces de gestionarlos de manera tal que se asegure su desempeño. Este artículo analiza la incidencia de la cultura de las organizaciones, su adaptabilidad al cambio y su capacidad de aprendizaje, como elementos claves para que estas puedan basar su gestión en proyectos, a la par que propone buenas prácticas que permitan incrementar sus posibilidades de éxito en este desafío.

The tsUL9 gene products exhibit a strongly reduced ATPase function at the nonpermissive temperature, an effect that can be compensated by the addition of unspecific DNA. Here it is demonstrated, that the reduced ATPase activity is caused by altered ATP binding of the tsOBP at the nonpermissive temperature. Furthermore the addition of unspecific DNA to the ATPase reaction not only compensates the ts phenotype at the nonpermissive temperature but also enhances the ATPase activity at the permissive temperature of both wild type and tsOBP. Thereby, the OBP becomes autophosphorylated. Moreover, addition of subgenomic HSV-1 DNA has no further enhancing effect, supporting the inchworm model for the HSV-OBP function. [ABSTRACT FROM AUTHOR]

Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae) is one of the preponderant egg parasitoids of Dendrolimus spp., which are important defoliators of coniferous forests. This parasitoid wasp has been widely released to control pine caterpillar and other lepidopteran pests, but its control efficiency needs to be improved. Sensory systems are crucial for T. dendrolimi to locate hosts, and immunity is probably involved after egg deposition in the host cavity; however, few reports have focused on the molecular mechanism of olfactory detection and survival of T. dendrolimi. It is necessary to identify these genes before further functional research is conducted. In this study, we assembled and analyzed the transcriptome of T. dendrolimi using next-generation sequencing technology. The sequencing and assembly resulted in 38 565 contigs with N50 of 3422 bp. Sequence comparison indicate that T. dendrolimi sequences are very similar to those of another parasitoid Nasonia vitripennis. Then the olfactory, vision, and immune-related gene families were identified, and phylogenetic analyses were performed with these genes from T. dendrolimi and other model insect species. Furthermore, phylogenetic tree with odorant binding proteins of T. dendrolimi and their host Dendrolimus was constructed to determine whether convergent evolution exists. These genes can be valid targets for further gene function research. The present study may help us to understand host location and survival mechanisms of T. dendrolimi and to use them more efficiently for pest control in the future. [ABSTRACT FROM AUTHOR]

High Throughput Sequencing capabilities have made the process of assembling a transcriptome easier, whether or not there is a reference genome. But the quality of a transcriptome assembly must be good enough to capture the most comprehensive catalog of transcripts and their variations, and to carry out further experiments on transcriptomics. There is currently no consensus on which of the many sequencing technologies and assembly tools are the most effective. Many non-model organisms lack a reference genome to guide the transcriptome assembly. One question, therefore, is whether or not a reference-based genome assembly gives better results than de novo assembly. The blood-sucking insect Rhodnius prolixus —a vector for Chagas disease—has a reference genome. It is therefore a good model on which to compare reference-based and de novo transcriptome assemblies. In this study, we compared de novo and reference-based genome assembly strategies using three datasets (454, Illumina, 454 combined with Illumina) and various assembly software. We developed criteria to compare the resulting assemblies: the size distribution and number of transcripts, the proportion of potentially chimeric transcripts, how complete the assembly was (completeness evaluated both through CEGMA software and R. prolixus proteome fraction retrieved). Moreover, we looked for the presence of two chemosensory gene families (Odorant-Binding Proteins and Chemosensory Proteins) to validate the assembly quality. The reference-based assemblies after genome annotation were clearly better than those generated using de novo strategies alone. Reference-based strategies revealed new transcripts, including new isoforms unpredicted by automatic genome annotation. However, a combination of both de novo and reference-based strategies gave the best result, and allowed us to assemble fragmented transcripts. [ABSTRACT FROM AUTHOR]

Butterflies and moths differ significantly in their daily activities: butterflies are diurnal while moths are largely nocturnal or crepuscular. This life history difference is presumably reflected in their sensory biology, and especially the balance between the use of chemical versus visual signals. Odorant Binding Proteins (OBP) are a class of insect proteins, at least some of which are thought to orchestrate the transfer of odor molecules within an olfactory sensillum (olfactory organ), between the air and odor receptor proteins (ORs) on the olfactory neurons. A Lepidoptera specific subclass of OBPs are the GOBPs and PBPs; these were the first OBPs studied and have well documented associations with olfactory sensilla. We have used the available genomes of two moths, Manduca sexta and Bombyx mori , and two butterflies, Danaus plexippus and Heliconius melpomene , to characterize the GOBP/PBP genes, attempting to identify gene orthologs and document specific gene gain and loss. First, we identified the full repertoire of OBPs in the M. sexta genome, and compared these with the full repertoire of OBPs from the other three lepidopteran genomes, the OBPs of Drosophila melanogaster and select OBPs from other Lepidoptera. We also evaluated the tissue specific expression of the M. sexta OBPs using an available RNAseq databases. In the four lepidopteran species, GOBP2 and all PBPs reside in single gene clusters; in two species GOBP1 is documented to be nearby, about 100 kb from the cluster; all GOBP/PBP genes share a common gene structure indicating a common origin. As such, the GOBP/PBP genes form a gene complex. Our findings suggest that (1) the lepidopteran GOBP/PBP complex is a monophyletic lineage with origins deep within Lepidoptera phylogeny, (2) within this lineage PBP gene evolution is much more dynamic than GOBP gene evolution, and (3) butterflies may have lost a PBP gene that plays an important role in moth pheromone detection, correlating with a shift from olfactory (moth) to visual (butterfly) communication, at least regarding long distance mate recognition. These findings will be clarified by additional lepidopteran genomic data, but the observation that moths and butterflies share most of the PBP/GOBP genes suggests that they also share common chemosensory-based behavioral pathways. [ABSTRACT FROM AUTHOR]

We extend the observed best prediction (OBP; Jiang, Nguyen, and Rao 2011) method to small area estimation when the responses are counts at the area level. We show via a simulation study that the OBP outperforms the empirical best prediction method when the underlying model is mis-specified. A bootstrap method is proposed for estimating the area-specific mean squared prediction error of the OBP conditioning on the small area mean counts. Two real data examples are considered. [ABSTRACT FROM AUTHOR]

Background: The complex societies of ants and other social insects rely on sophisticated chemical communication. Two families of small soluble proteins, the odorant binding and chemosensory proteins (OBPs and CSPs), are believed to be important in insect chemosensation. To better understand the role of these proteins in ant olfaction, we examined their evolution and expression across the ants using phylogenetics and sex- and tissue-specific RNA-seq. Results: We find that subsets of both OBPs and CSPs are expressed in the antennae, contradicting the previous hypothesis that CSPs have replaced OBPs in ant olfaction. Both protein families have several highly conserved clades with a single ortholog in all eusocial hymenopterans, as well as clades with more dynamic evolution and many taxon-specific radiations. The dynamically evolving OBPs and CSPs have been hypothesized to function in chemical communication. Intriguingly, we find that seven members of the conserved clades are expressed specifically in the antennae of the clonal raider ant Cerapachys biroi, whereas only one dynamically evolving CSP is antenna specific. The orthologs of the conserved, antenna-specific C. biroi genes are also expressed in antennae of the ants Camponotus floridanus and Harpegnathos saltator, indicating that antenna-specific expression of these OBPs and CSPs is conserved across ants. Most members of the dynamically evolving clades in both protein families are expressed primarily in non-chemosensory tissues and thus likely do not fulfill chemosensory functions. Conclusions: Our results identify candidate OBPs and CSPs that are likely involved in conserved aspects of ant olfaction, and suggest that OBPs and CSPs may not rapidly evolve to recognize species-specific signals. [ABSTRACT FROM AUTHOR]

The global positioning system (GPS) is one of the most powerful tools available for observation of Earth's surface deformation. In particular, coseismic, postseismic, slow transient, and interseismic deformation have all been observed globally by GPS over the past two decades, especially in subduction zones. Moreover, GPS-acoustic techniques have been developed for practical use in the past decade, allowing observation of offshore deformation immediately above slip regions. Here, we describe the application of GPS and GPS-acoustic observations to the detection of deformation due to plate boundary slip for interplate earthquakes as well as afterslip and slow slip events in subduction zones around Japan, where geodetic data coverage is particularly dense. The data demonstrate temporally variable strain accumulation in the source region of the 2011 Mw 9.0 Tohoku-oki earthquake, and observation of the huge slip of the Tohoku-oki earthquake near the trench using GPS-acoustic methods has considerably advanced our knowledge of stress release and accumulation in this subduction zone. [ABSTRACT FROM AUTHOR]

Since the publication of Michael Lewis's book, Moneyball, there has been a commonly held belief that getting on-base percentage (OBP) is more important in run production than hitting for power slugging percentage (SLG). Implicit in that conclusion is an assumption that OBP and SLG are drawn from similar distributions. The author finds, however, that they are drawn from meaningfully different distributions. Controlling for those differences, the author finds no evidence that OBP is more important in run production than SLG. In fact, the author finds that from 1980 to 2007 SLG was more important in run production than OBP. [ABSTRACT FROM PUBLISHER]

Background Aedes aegypti mosquitoes are the main vectors of dengue viruses. Despite global efforts to reduce the prevalence of dengue using integrated vector management strategies, innovative alternatives are necessary to help prevent virus transmission. Detailed characterizations of Ae. aegypti genes and their products provide information about the biology of mosquitoes and may serve as foundations for the design of new vector control methods. Findings We studied the Ae. aegypti gene, AAEL010714, that encodes a two-domain odorant-binding protein, AaegOBP45. The predicted gene structure and sequence were validated, although single nucleotide polymorphisms were observed. Transcriptional and translational products accumulate in the ovaries of blood fed females and are not detected or are at low abundance in other tissues. Conclusions We validated the Ae. aegypti AAEL010714 gene sequence and characterized the expression profile of a two-domain OBP expressed in ovaries. We propose that AaegOBP45 function as a component of the mosquito eggshell. [ABSTRACT FROM AUTHOR]

About 1 million people in the world die each year from diseases spread by mosquitoes, and understanding the mechanism of host identification by the mosquitoes through olfaction is at stake. The role of odorant binding proteins (OBPs) in the primary molecular events of olfaction in mosquitoes is becoming an important focus of biological research in this area. Here, we present a comprehensive comparative genomics study of OBPs in the three disease-transmitting mosquito species Anopheles gambiae, Aedes aegypti, and Culex quinquefasciatus starting with the identification of 110 new OBPs in these three genomes. We have characterized their genomic distribution and orthologous and phylogenetic relationships. The diversity and expansion observed with respect to the Aedes and Culex genomes suggests that the OBP gene family acquired functional diversity concurrently with functional constraints posed on these two species. Sequences with unique features have been characterized such as the “two-domain OBPs” (previously known as Atypical OBPs) and “MinusC OBPs” in mosquito genomes. The extensive comparative genomics featured in this work hence provides useful primary insights into the role of OBPs in the molecular adaptations of mosquito olfactory system and could provide more clues for the identification of potential targets for insect repellants and attractants. [ABSTRACT FROM AUTHOR]

Chemoreception is a biological process essential for the survival of animals, as it allows the recognition of important volatile cues for the detection of food, egg-laying substrates, mates, or predators, among other purposes. Furthermore, its role in pheromone detection may contribute to evolutionary processes, such as reproductive isolation and speciation. This key role in several vital biological processes makes chemoreception a particularly interesting system for studying the role of natural selection in molecular adaptation. Two major gene families are involved in the perireceptor events of the chemosensory system: the odorantbinding protein (OBP) and chemosensory protein (CSP) families. Here, we have conducted an exhaustive comparative genomic analysis of these gene families in 20 Arthropoda species. We show that the evolution of the OBP and CSP gene families is highly dynamic, with a high number of gains and losses of genes, pseudogenes, and independent origins of subfamilies. Taken together, our data clearly support the birth-and-death model for the evolution of these gene families with an overall high gene turnover rate. Moreover, we show that the genome organization of the two families is significantly more clustered than expected by chance and, more important, that this pattern appears to be actively maintained across the Drosophila phylogeny. Finally, we suggest the homologous nature of the OBP and CSP gene families, dating back their most recent common ancestor after the terrestrialization of Arthropoda (380–450 Ma) and we propose a scenario for the origin and diversification of these families. [ABSTRACT FROM AUTHOR]