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Your search keyword '"null Jianmin Wang"' showing total 2 results
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2 results on '"null Jianmin Wang"'

1. Author response for 'Rocky Mountain forests are poised to recover following bark beetle outbreaks, but with altered composition'

Author

null Kyle C. Rodman, null Robert A. Andrus, null Amanda R. Carlson, null Trevor A. Carter, null Teresa B. Chapman, null Jonathan D. Coop, null Paula J. Fornwalt, null Nathan S. Gill, null Brian J. Harvey, null Ashley E. Hoffman, null Katharine C. Kelsey, null Dominik Kulakowski, null Daniel C. Laughlin, null Jenna E. Morris, null José F. Negrón, null Katherine M. Nigro, null Gregory S. Pappas, null Miranda D. Redmond, null Charles C. Rhoades, null Monique E. Rocca, null Zoe H. Schapira, null Jason S. Sibold, null Camille S. Stevens‐Rumann, null Thomas T. Veblen, null Jianmin Wang, null Xiaoyang Zhang, and null Sarah J. Hart

Published
2022
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2. Isolation and Characterization of Matrix Attachment Regions from Green Alga:Dunaliella salina

Author

null Tianyun Wang, null Yurong Chai, null Weihong Hou, null Jianmin Wang, and null Lexun Xue

Subjects
biology, biology.organism_classification, Nuclear matrix, Yeast, Microbiology, Restriction enzyme, chemistry.chemical_compound, Algae, Biochemistry, chemistry, Gene expression, Dunaliella salina, Scaffold/matrix attachment region, DNA
Abstract

Matrix attachment region (MAR) is the DNA fragment that could bind to the nuclear matrix. Recently, many experiments have demonstrated that MAR can increase overall levels of foreign gene expression and decrease variability of expression. MARs have been isolated from yeast, plants, animals and human, but not from algae. In order to isolation the MAR from unicellular alga: Dunaliella salina, we first created a random MAR library as follows: The nuclear matrix were prepared from isolated nuclei by extracting with 25mM lithium diiodosalicylate (LIS) and then digested with restriction enzyme, the residual DNA fragments in the nuclear matrices were cloned into the pUC18 to construct the MAR library. Secondly, the interactions of several of the cloned DNA fragments with nuclear matrices were tested by an in vitro binding assay in which E.coli DNA was used as competitor. Some of DNA fragments could bind specifically to the nuclear matrices and four of them contained sequences related to known consensus motifs and a hairpin loop structure of MAR.

Published
2004
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