1. Non-Affinity Purification of Antibodies.
- Author
-
Arakawa, Tsutomu, Tomioka, Yui, Nakagawa, Masataka, Sakuma, Chiaki, Kurosawa, Yasunori, Ejima, Daisuke, Tsumoto, Kouhei, and Akuta, Teruo
- Subjects
- *
IMMUNOGLOBULINS , *COLUMN chromatography , *GEL electrophoresis , *AGAROSE - Abstract
Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review. As an alternative, we propose a simple small-scale purification protocol without Protein A that uses novel agarose native gel electrophoresis and protein extraction. For large-scale antibody purification, we suggest mixed-mode chromatography that can in part mimic the properties of Protein A resin, focusing on 4-Mercapto-ethyl-pyridine (MEP) column chromatography. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF