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3 results on '"neural transcriptional dynamics"'

1. Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates

Author

Nicola Micali, Suel-Kee Kim, Marcelo Diaz-Bustamante, Genevieve Stein-O’Brien, Seungmae Seo, Joo-Heon Shin, Brian G. Rash, Shaojie Ma, Yanhong Wang, Nicolas A. Olivares, Jon I. Arellano, Kristen R. Maynard, Elana J. Fertig, Alan J. Cross, Roland W. Bürli, Nicholas J. Brandon, Daniel R. Weinberger, Joshua G. Chenoweth, Daniel J. Hoeppner, Nenad Sestan, Pasko Rakic, Carlo Colantuoni, and Ronald D. McKay

Subjects
neural stem cell, FGF2, BMP, neural transcriptional dynamics, neurogenesis, EGFR, Biology (General), QH301-705.5
Abstract

Summary: Better understanding of the progression of neural stem cells (NSCs) in the developing cerebral cortex is important for modeling neurogenesis and defining the pathogenesis of neuropsychiatric disorders. Here, we use RNA sequencing, cell imaging, and lineage tracing of mouse and human in vitro NSCs and monkey brain sections to model the generation of cortical neuronal fates. We show that conserved signaling mechanisms regulate the acute transition from proliferative NSCs to committed glutamatergic excitatory neurons. As human telencephalic NSCs develop from pluripotency in vitro, they transition through organizer states that spatially pattern the cortex before generating glutamatergic precursor fates. NSCs derived from multiple human pluripotent lines vary in these early patterning states, leading differentially to dorsal or ventral telencephalic fates. This work furthers systematic analyses of the earliest patterning events that generate the major neuronal trajectories of the human telencephalon.

Published
2020
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2. Variation of human neural stem cells generating organizer statesin vitrobefore committing to cortical excitatory or inhibitory neuronal fates

Author

Daniel R. Weinberger, Marcelo Diaz-Bustamante, Nicolas A. Olivares, Pasko Rakic, Suel Kee Kim, Roland Bürli, Seungmae Seo, Shaojie Ma, Nicholas J. Brandon, Josh G. Chenoweth, Nicola Micali, Daniel J. Hoeppner, Elana J. Fertig, Yanhong Wang, Jon I. Arellano, Brian G. Rash, Genevieve Stein-O’Brien, Alan J. Cross, Kristen R. Maynard, Carlo Colantuoni, Nenad Sestan, Joo Heon Shin, and Ronald D.G. McKay

Subjects
0301 basic medicine, FGF2, EGFR, Neurogenesis, Induced Pluripotent Stem Cells, Biology, Inhibitory postsynaptic potential, Article, General Biochemistry, Genetics and Molecular Biology, neural stem cell, 03 medical and health sciences, Glutamatergic, 0302 clinical medicine, Neural Stem Cells, medicine, BMP, Humans, lcsh:QH301-705.5, Cells, Cultured, Embryonic Stem Cells, reproductive and urinary physiology, 030304 developmental biology, Neurons, 0303 health sciences, Cerebrum, Cell Differentiation, Neural stem cell, Cortex (botany), 030104 developmental biology, medicine.anatomical_structure, lcsh:Biology (General), nervous system, neural transcriptional dynamics, Cerebral cortex, Excitatory postsynaptic potential, Neuroscience, 030217 neurology & neurosurgery, Signal Transduction
Abstract

SUMMARY Better understanding of the progression of neural stem cells (NSCs) in the developing cerebral cortex is important for modeling neurogenesis and defining the pathogenesis of neuropsychiatric disorders. Here, we use RNA sequencing, cell imaging, and lineage tracing of mouse and human in vitro NSCs and monkey brain sections to model the generation of cortical neuronal fates. We show that conserved signaling mechanisms regulate the acute transition from proliferative NSCs to committed glutamatergic excitatory neurons. As human telencephalic NSCs develop from pluripotency in vitro, they transition through organizer states that spatially pattern the cortex before generating glutamatergic precursor fates. NSCs derived from multiple human pluripotent lines vary in these early patterning states, leading differentially to dorsal or ventral telencephalic fates. This work furthers systematic analyses of the earliest patterning events that generate the major neuronal trajectories of the human telencephalon., In Brief Micali et al. report that human telencephalic NSCs in vitro transition through the organizer states that pattern the neocortex. Human pluripotent lines vary in organizer formation, generating divergent neuronal differentiation trajectories biased toward dorsal or ventral telencephalic fates and opening further analysis of the earliest cortical specification events., Graphical Abstract

Published
2019

3. Variation of Human Neural Stem Cells Generating Organizer States In Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates.

Author

Micali N, Kim SK, Diaz-Bustamante M, Stein-O'Brien G, Seo S, Shin JH, Rash BG, Ma S, Wang Y, Olivares NA, Arellano JI, Maynard KR, Fertig EJ, Cross AJ, Bürli RW, Brandon NJ, Weinberger DR, Chenoweth JG, Hoeppner DJ, Sestan N, Rakic P, Colantuoni C, and McKay RD

Subjects
Cell Differentiation physiology, Cells, Cultured, Humans, Induced Pluripotent Stem Cells metabolism, Signal Transduction physiology, Embryonic Stem Cells metabolism, Neural Stem Cells cytology, Neurogenesis physiology, Neurons metabolism
Abstract

Better understanding of the progression of neural stem cells (NSCs) in the developing cerebral cortex is important for modeling neurogenesis and defining the pathogenesis of neuropsychiatric disorders. Here, we use RNA sequencing, cell imaging, and lineage tracing of mouse and human in vitro NSCs and monkey brain sections to model the generation of cortical neuronal fates. We show that conserved signaling mechanisms regulate the acute transition from proliferative NSCs to committed glutamatergic excitatory neurons. As human telencephalic NSCs develop from pluripotency in vitro, they transition through organizer states that spatially pattern the cortex before generating glutamatergic precursor fates. NSCs derived from multiple human pluripotent lines vary in these early patterning states, leading differentially to dorsal or ventral telencephalic fates. This work furthers systematic analyses of the earliest patterning events that generate the major neuronal trajectories of the human telencephalon., Competing Interests: Declaration of Interests The authors declare no competing interests., (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)

Published
2020
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