1. Cell Membrane-Based Nanoreactor To Mimic the Bio-Compartmentalization Strategy of a Cell
- Author
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Hongbo Zhang, Hélder A. Santos, Andrea Poillucci, Alexandra Correia, Vimalkumar Balasubramanian, Christian Celia, Faculty of Pharmacy, Division of Pharmaceutical Chemistry and Technology, Drug Research Program, Preclinical Drug Formulation and Analysis group, and Nanomedicines and Biomedical Engineering
- Subjects
ta221 ,Cell ,Biomedical Engineering ,ENZYMATIC-REACTIONS ,LIPOSOMES ,enzyme reaction ,self-assembled membrane vesicles ,02 engineering and technology ,Nanoreactor ,010402 general chemistry ,01 natural sciences ,Horseradish peroxidase ,Article ,VESICLES ,Biomaterials ,Cell membrane ,RED-BLOOD-CELLS ,colloidal nanoparticles ,BIODISTRIBUTION ,Organelle ,medicine ,ta318 ,DRUG-DELIVERY ,nanoerythrosomes ,biology ,CATALYSIS ,Chemistry ,Vesicle ,HORSERADISH-PEROXIDASE ,021001 nanoscience & nanotechnology ,PARTICLE-SIZE ,POLYMERIC NANOPARTICLES ,0104 chemical sciences ,medicine.anatomical_structure ,Membrane ,317 Pharmacy ,Enzyme model ,Biophysics ,biology.protein ,1182 Biochemistry, cell and molecular biology ,0210 nano-technology ,nanoparticle reactors - Abstract
Organelles of eukaryotic cells are structures made up of membranes, which carry out a majority of functions necessary for the surviving of the cell itself. Organelles also differentiate the prokaryotic and eukaryotic cells, and are arranged to form different compartments guaranteeing the activities for which eukaryotic cells are programmed. Cell membranes, containing organelles, are isolated from cancer cells and erythrocytes and used to form biocompatible and long circulating ghost nanoparticles delivering payloads or catalyzing enzymatic reactions as nanoreactors. In this attempt, red blood cell membranes were isolated from erythrocytes, and engineered to form nanoerythrosomes (NERs) of 150 nm. The horseradish peroxidase, used as an enzyme model, was loaded inside the aqueous compartment of NERs, and its catalytic reaction with Resorufm was monitored. The resulting nanoreactor protected the enzyme from proteolytic degradation, and potentiated the enzymatic reaction in situ as demonstrated by maximal velocity (V-max) and Michaelis constant (K-m), thus suggesting the high catalytic activity of nanoreactors compared to the pure enzymes.
- Published
- 2018
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