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Background: Vitamin B12 is a crucial B-group vitamin, first isolated from the liver due to its role in combating pernicious anemia. It is distinguished by its unique and complex structure, which makes its chemical synthesis challenging and expensive. Consequently, vitamin B12 is alternatively obtained through microbial fermentations. Molasses, an affordable and safe agro-industrial waste, can be used as a carbon source for vitamin B12 production, offering a cost-effective alternative to expensive sugars in the production medium. Results: A total of 87 yeast, actinomycete, and bacterial isolates were screened for vitamin B12 production, with 15 isolates showing high productivity. Bacillus isolates were selected for further analysis using MALDI-TOF and molecular identification. These isolates were identified as four strains of Bacillus subtilis (MZ08, JT10, BY11, and JT17), one strains of Bacillus sp. (CB09), and one strain of Peribacillus acanthi (MZ01). Genetic circuits associated with vitamin B12 production were demonstrated in a closely related strain of Peribacillus acanthi MZ01 strain. Three strains (MZ01, MZ08, and JT17) were selected for further evaluation of vitamin B12 productivity under different sugar types (glucose, sucrose, fructose, lactose, and galactose) and varying inoculum sizes. The inoculum size significantly impacted vitamin B12 production, with an increase from 5 to 10% enhancing yields. The ability of the strains to produce vitamin B12 varied depending on the type of sugar used. Peribacillus acanthi MZ01 strain showed the highest productivity and subsequently, selected for optimizing vitamin B12 production conditions using response surface methodology. Furthermore, the optimized conditions were then applied to molasses-based medium to achieve high vitamin B12 yields by MZ01 strain. Conclusion: In this study, Peribacillus acanthi was characterized for the first time as a vitamin B12 producer, demonstrating high productivity among various tested strains. The optimization of production conditions using response surface methodology, further enhanced vitamin B12 yields, showcasing the strain's efficiency in microbial fermentations. This research also highlights the potential of using molasses as a cost-effective alternative carbon source, significantly reducing production costs. [ABSTRACT FROM AUTHOR]

Interest in biosurfactants application has increased considerably due to unique properties, such as diversity, biodegradability and environmentally friendly concerns. Therefore, using abundant and low-cost raw materials and optimizing the biosurfactant production process are economically attractive. In this study, the suitability of sugar beet molasses was compared with some other common carbon sources for rhamnolipids production by Pseudomonas putida KT2440. The effects of different inorganic nitrogen sources, C/N ratios, and incubation time were also investigated to optimize biosurfactant production. The results showed that the maximum biosurfactant precipitated in acetone (5.4 g.L-1) was obtained using molasses and sodium nitrate. In the application of these carbon and nitrogen sources, minimum surface tension was established at C/N ratio of 10 and 8 days of incubation time. Affected by various sources of carbon and nitrogen, the final pH values varied between 2.9 and 8.3. Although the minimum surface tension (41.78 m.Nm-1) was measured using olive oil and ammonium chloride, this treatment did not produce the maximum biosurfactant weight. The minimum pH of cell-free supernatants was related to this treatment. In other words, the final pH of the culture medium was effective in measuring parameters related to biosurfactant. Acetone-extracted biosurfactant was pH-dependent and increased with the increasing pH of the culture mediums. The results indicated the potential of sugar beet molasses as a hydrophilic agro-industrial wastes to rhamnolipid production by Pseudomonas putida KT2440. [ABSTRACT FROM AUTHOR]

Background: Formaldehyde is a reactive chemical that bonds randomly with various cellular elements. This substance, which is also found in the natural structure of the organism, is used in many areas, from industry to household materials, from the production of coatings in dentistry to the fixation of cadavers in laboratories. Formaldehyde is commonly used in laboratories to fix tissues. Objectives: In this study, the staining properties of tissues fixed with date molasses, a natural sugar, using the May-Grunwald-Giemsa (MGG) staining method were compared with the staining properties of tissues fixed with buffered formol-saline. Methods: Liver, spleen, kidney, skin, testicle, small intestine, large intestine, brain, cerebellum, and lung tissue samples of 4 rats of both genders were used as materials in the study. Each tissue underwent routine histological tissue tracing and was stained with MGG. Results: When all tissues were evaluated anatomically, the general morphology of the tissues showed that the tissue integrity was preserved. There was no color change in the tissues. In addition, tissue hardness was at the required level, supporting previous studies. Histologically, regarding chromatin distribution, nucleus separation and cytoplasm staining, tissues fixed with date molasses and tissues fixed with buffered formal saline showed similar properties, except for the tissues subjected to intense maceration (skin and small intestines). Pale staining was observed in some tissues fixed with buffered formal saline (liver, skin, heart), but the nucleus and cytoplasm structures were better distinguished at higher magnifications. Considering the chromatin distribution, nuclear separation and cytoplasm staining in other tissues (spleen, kidney, testicle, large intestine, brain, cerebellum and lung), tissues fixed with date molasses showed similar properties to tissues fixed with buffered formal-saline. Conclusion: We recommend that researchers interested in the subject prolong the fixation period (6 months) and compare these results with those of tissues fixed for 24 hours. [ABSTRACT FROM AUTHOR]

Sugar beet is important for amino acids like glycine betaine beside sugar because it has medicinal effects in poultry, fish and also in human as it is used for anti-cancer drugs. This design was conducted in order to optimize betaine extraction from sugar beet molasses in ion exchange chromatography method. Firstly, an experiment was conducted in factorial based on completely randomized design with three replications for optimizing suitable extraction condition of different injected materials to chromatography column. The first factor was injected materials including potassium chloride, pure sucrose and betaine, the second factor was related to column temperature including 25, 50 and 75°C and the third factor was different resins including experimental, commercial 1 and 2 resins. The extracted volumes of these injected materials were significantly different. The best temperature for betaine extraction was 75°C.the best resin for betaine extraction was commercial resin 1 in which betaine was extracted with more quantity and efficiency and faster in comparison with other resins. Secondly betaine of sugar beet molasses was extracted based on optimum condition at first step. The purity of extracted betaine was 54, 64 and 79% with mean of 65.66. This experiment was conducted in experimental level which would be useful for performing at industrial level in different sugar factories of the country to extract betaine from sugar beet molasses. [ABSTRACT FROM AUTHOR]

Globally escalating ethanol demand necessitates the use of hybrid technologies integrating first- and second-generation biofuel feedstocks for achieving the futuristic targets of gasoline replacement with bioethanol. In present study, an optimized two-step sequential pre-treatment (first dilute alkali, then dilute acid) of Pine forest litter (PFL) was developed. Furthermore, the saccharification of pre-treated PFL was optimized through Response Surface Methodology using Box-Behnken Design, wherein 0.558 g/g of reducing sugar was released under the optimized conditions (12.5% w/v of biomass loading, 10 FPU/g of PFL enzyme loading, 0.15% v/v Tween-80 and 48 h incubation time). Moreover, during hydrolysate fermentation using Saccharomyces cerevisiae NCIM 3288 strain, 22.51 ± 1.02 g/L ethanol was produced. Remarkably, hydrophobic resin (XAD-4) treatment of PFL hydrolysate, significantly removed inhibitors (Furfural, 5-hydroxymethylfurfural and phenolics) and increased ethanol production to 27.38 ± 1.18 g/L. Furthermore, during fermentation of molasses supplemented PFL hydrolysate (total initial sugar: 100 ± 3.27 g/L), a maximum of 46.02 ± 2.08 g/L ethanol was produced with 0.482 g/g yield and 1.92 g/l/h productivity. These findings indicated that the integration of molasses to lignocellulosic hydrolysate, would be a promising hybrid technology for industrial ethanol production within existing bio-refinery infrastructure. [ABSTRACT FROM AUTHOR]

Biomass from willow and poplar harvested for feed during the growing season may be preserved by ensiling; however, little research has focused on ensiling of these biomasses. This study focuses on the use of ensiling additives to reduce the pH to around 4.0 to secure stable storage. Lab-scale ensiling experiments were conducted with different willow and poplar clones, shoot ages, and harvest times (June or September). Ensiling without additives often resulted in limited pH reduction. The pH could be reduced in the biomass of both species by adding formic acid, and the required dose to reduce the pH to 4.0 (buffering capacity, BC) ranged significantly between biomass types but was in the range of 2–5 kg formic acid (78%) per ton fresh weight. BC decreased with increasing dry matter (DM) content and decreasing crude protein content. The pH could also be reduced during ensiling by applying molasses and/or lactic acid bacteria, although not sufficiently in poplar. Willow biomass was ensiled effectively at the pilot scale with less than 7% DM loss by adding formic acid or by mixing with grass biomass. Comparable pH results were obtained at the lab scale and pilot scale. The study demonstrates how willow and poplar can be ensiled; however, more research is needed on quality changes during ensiling. [ABSTRACT FROM AUTHOR]

The widespread adoption of Poly(3-hydroxybutyrate) (PHB) encounters challenges due to its higher production costs compared to conventional plastics. To overcome this obstacle, this study investigates the use of low-cost raw materials and optimized production methods. Specifically, food processing byproducts such as corn germ and corn bran were utilized as solid substrates through solid-state fermentation, enriched with molasses and cheese whey. Employing the One Factor at a Time technique, we examined the effects of substrate composition, temperature, initial substrate moisture, molasses, and cheese whey on PHB production at the flask scale. Subsequently, experiments were conducted at the bioreactor scale to evaluate the influence of aeration. In flask-scale experiments, the highest PHB yield, reaching 4.1 (g/kg Initial Dry Weight Substrate) (IDWS) after 72 hours, was achieved using a substrate comprising a 1:1 mass ratio of corn germ to corn bran supplemented with 20 % (v/w) cheese whey. Furthermore, PHB production in a 0.5-L packed-bed bioreactor yielded a maximum of 8.4 (g/kg IDWS), indicating a more than 100 % increase in yield after 72 hours, with optimal results achieved at an aeration rate of 0.5 l/(kg IDWS. h). • PHB was synthesized using a mixture of agricultural and food waste substrates. • The impact of operational variables on PHA production in SSF was determined. • Temperature, moisture, substrate composition and cheese whey affected on process. • SSF was scaled up to a packed-bed bioreactor to investigate aeration rate effect. • Maximum PHB production reached 8.4 (g/kg IDWS) using Cupriavidus necator. [ABSTRACT FROM AUTHOR]

Combined drying, an energy-efficient method that includes osmotic pretreatment in molasses and shortened successive lyophilization, was used to obtain celery root powder and incorporate it in the formulation of cookies, with the aim of obtaining a new product. Wheat flour was substituted with combinedly dehydrated celery root powder at levels from 0 to 30%, and optimization of the amount of wheat flour substitution regarding technological, sensory and nutritive characteristics was performed. The optimal level of 20% substitution was determined using Z-score analysis, from the aspect of the best nutritive improvement and the mildest adverse impact on the technological and sensory quality. In the second research phase, comparison of the cookies with the 20% celery root powder substitution, dehydrated by different methods, indicated that combined dehydration showed upgraded results in terms of the overall quality of the final product, for 28.85 percentile points higher than cookies with lyophilized and for 65.24 percentile points higher than cookies with the addition of convectively dried celery root powder. The cookie containing celery powder previously osmodehydrated in molasses had higher contents of analyzed minerals (1.2–3.3 times), total phenols (10.8%) and antioxidant activities (14% for DPPH and 4% for ABTS) compared to the cookie with lyophilized powder. [ABSTRACT FROM AUTHOR]

Abstract Background Vitamin B12 is a crucial B-group vitamin, first isolated from the liver due to its role in combating pernicious anemia. It is distinguished by its unique and complex structure, which makes its chemical synthesis challenging and expensive. Consequently, vitamin B12 is alternatively obtained through microbial fermentations. Molasses, an affordable and safe agro-industrial waste, can be used as a carbon source for vitamin B12 production, offering a cost-effective alternative to expensive sugars in the production medium. Results A total of 87 yeast, actinomycete, and bacterial isolates were screened for vitamin B12 production, with 15 isolates showing high productivity. Bacillus isolates were selected for further analysis using MALDI-TOF and molecular identification. These isolates were identified as four strains of Bacillus subtilis (MZ08, JT10, BY11, and JT17), one strains of Bacillus sp. (CB09), and one strain of Peribacillus acanthi (MZ01). Genetic circuits associated with vitamin B12 production were demonstrated in a closely related strain of Peribacillus acanthi MZ01 strain. Three strains (MZ01, MZ08, and JT17) were selected for further evaluation of vitamin B12 productivity under different sugar types (glucose, sucrose, fructose, lactose, and galactose) and varying inoculum sizes. The inoculum size significantly impacted vitamin B12 production, with an increase from 5 to 10% enhancing yields. The ability of the strains to produce vitamin B12 varied depending on the type of sugar used. Peribacillus acanthi MZ01 strain showed the highest productivity and subsequently, selected for optimizing vitamin B12 production conditions using response surface methodology. Furthermore, the optimized conditions were then applied to molasses-based medium to achieve high vitamin B12 yields by MZ01 strain. Conclusion In this study, Peribacillus acanthi was characterized for the first time as a vitamin B12 producer, demonstrating high productivity among various tested strains. The optimization of production conditions using response surface methodology, further enhanced vitamin B12 yields, showcasing the strain’s efficiency in microbial fermentations. This research also highlights the potential of using molasses as a cost-effective alternative carbon source, significantly reducing production costs.

This study endeavoured to capitalize on prickly pear by-products for the optimization of molasses formulation, targeting elevated antioxidant capacities and superior sugar contents. Through robust statistical modelling, the optimal cooking parameters—temperature (70-80 °C) and duration (60–90 min)—were determined, guided by responses of antioxidant activity and Brix value. A D-Optimal mixture design further delineated the ideal proportions of molasses components (pulp, peel, and seeds). Characterization revealed that peel harboured the highest concentrations of total polyphenols (396.41 mg GAE/100g FW) and flavonoids (234.26 mg CE/100g FW), emphasizing its antioxidant potential (DPPH inhibition IC50: 12.72 µg/ml). The optimal cooking conditions were established at 78.35 °C for 79.70 min, with predictive equations guiding ingredient proportions (0.265 g pulp, 0.710 g peel, 0.025 g seed). Intriguingly, while peel inclusion enhanced total sugar content and antioxidant activity, seed incorporation exerted a contrasting effect by reducing total sugar content and limiting antioxidant activity. [ABSTRACT FROM AUTHOR]

The article presents the results of experimental studies on the production of fuel briquettes from carbon-containing technogenic raw materials with a certain energy potential, flotation concentrate of coal enrichment sludge and wood and plant residues with binders. The technical characteristics of fuel briquettes of experimental batches are presented. The applicability of the selected binders for obtaining high-quality fuel briquettes was demonstrated. Aspects of the formation of the structure and properties of fuel briquettes are considered. [ABSTRACT FROM AUTHOR]

Abstract This study explores the differentiation of sugar and molasses produced from sugar beet and cane, which are susceptible to fraudulent labeling due to differing production costs. The research aimed to authenticate these products by botanical origin using novel analytical techniques. Utilizing ethanol isotopic measurement–isotope ratio mass spectrometry (IRMS) for non‐exchangeable hydrogen stable isotopes alongside carbon stable isotopes analysis through elemental analyzer–IRMS, the study accurately identified the origin of various sugar and molasses samples, pinpointed mislabeled goods, and determined the source of products with previously unknown provenance. These methods were also effective in revealing sugar and molasses adulteration and quantifying the extent of such fraud. The combined isotope analyses demonstrated their potential as robust tools for combating misrepresentation and adulteration in the sugar industry.

Abstract Background An important step in replacing petrochemical products with sustainable, cost-effective alternatives is the use of feedstocks other than, e.g., pure glucose in the fermentative production of platform chemicals. Ustilaginaceae offer the advantages of a wide substrate spectrum and naturally produce a versatile range of value-added compounds under nitrogen limitation. A promising candidate is the dicarboxylic acid malic acid, which may be applied as an acidulant in the food industry, a chelating agent in pharmaceuticals, or in biobased polymer production. However, fermentable residue streams from the food and agricultural industry with high nitrogen content, e.g., sugar beet molasses, are unsuited for processes with Ustilaginaceae, as they result in low product yields due to high biomass and low product formation. Results This study uncovers challenges in evaluating complex feedstock applicability for microbial production processes, highlighting the role of secondary substrate limitations, internal storage molecules, and incomplete assimilation of these substrates. A microliter-scale screening method with online monitoring of microbial respiration was developed using malic acid production with Ustilago trichophora on molasses as an application example. Investigation into nitrogen, phosphate, sulphate, and magnesium limitations on a defined minimal medium demonstrated successful malic acid production under nitrogen and phosphate limitation. Furthermore, a reduction of nitrogen and phosphate in the elemental composition of U. trichophora was revealed under the respective secondary substrate limitation. These adaptive changes in combination with the intricate metabolic response hinder mathematical prediction of product formation and make the presented screening methodology for complex feedstocks imperative. In the next step, the screening was transferred to a molasses-based complex medium. It was determined that the organism assimilated only 25% and 50% of the elemental nitrogen and phosphorus present in molasses, respectively. Due to the overall low content of bioavailable phosphorus in molasses, the replacement of the state-of-the-art nitrogen limitation was shown to increase malic acid production by 65%. Conclusion The identification of phosphate as a superior secondary substrate limitation for enhanced malic acid production opens up new opportunities for the effective utilization of molasses as a more sustainable and cost-effective substrate than, e.g., pure glucose for biobased platform chemical production.

The fabrication of charcoal briquettes was considered using two distinct bases: palmyra palm and oil palm shells. The critical role of binders – namely tapioca starch, molasses, and termite mound clay (TMC) – were emphasized in influencing the properties of the briquettes. ANOVA results revealed that both the type of binder and charcoal significantly impacted various characteristics, such as proximate components like volatile matter content, and physical properties including combustion time. Briquettes made from palmyra palm shells notably demonstrated superior performance in terms of combustion time and onset time of saturation (OTS). Among the binders, tapioca starch was distinguished for contributing to the lowest ash content and the highest fixed carbon in the briquettes. Conversely, briquettes bound with TMC, despite having the lowest volatile matter percentage, also exhibited the highest ash content and fragility, in addition to the shortest combustion time. These findings highlight the importance of selecting appropriate binders to enhance the efficiency and sustainability of charcoal briquettes, aligning with the increasing demand for environmentally conscious energy solutions in the face of escalating global energy needs.

A factorial experiment was conducted at the Research Station of the College of Agriculture, Anbar University, using the RCBD factorial experiments to determine the effect of the type of carrier and bacterial inoculum on the growth and yield of potatoes. Three carrier materials were used: pre-ripened date seeds, molasses, and zeolite as a control. B. megaterium and A. lipoferum carriers and inoculum were prepared and incubated for five days. The treatments were distributed randomly, adding the full fertilizer recommendation to the field, except P and N, which were added at a rate of 60% of the recommendation. The results showed that the carrier made from pre-ripe date seeds was superior to the zeolite, as the highest plant height and potato yield were recorded at 73.22 cm and 42.95 tons ha-1, with a significant difference from the lowest rate recorded for zeolite, 67.17 cm and 38.18 tons ha-1, respectively. Dpbm and molasses carriers recorded the highest percentages of N and P in soil, reaching 47.0, 45.98, 11.77, and 11.18 mg kg-1 soil, respectively. B. megaterium recorded the highest yields of potato and phosphorus in the soil, reaching 44 ton ha-1 and 9.86 mg Pkg-1. The interaction DpbB-B. megaterium recorded the highest average soil microbial content at 8.65 log cfu g-1. The molasses-A followed the lipoferum interaction with an average microbial content of 6.831 and 6.35.65 log cfu g-1.

Bacterial nanocellulose (BNC) is a unique biopolymer with remarkable purity and material properties that distinguish it from plant-derived cellulose by its absence of lignin and hemicellulose. Recent research has focused on using organic waste materials as alternative substrates to reduce the cost of BNC production. This study examines the efficacy of Siwalan neer and sugarcane molasses as novel media for cultivating Komagataeibacter xylinus by comparing their performance with that of Hestrin and Schramm (HS) medium. The BNC harvested from these alternative media was rigorously characterized using scanning electron microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), and Thermogravimetric Analysis (TGA) to evaluate its structural and thermal properties. Notably, the use of Siwalan neer and sugarcane molasses as growth substrates enabled K. xylinus to produce BNC at a yield of 3.83 g/L, significantly exceeding the yield from the HS medium, which was 1.46 g/L. These findings highlight the potential of leveraging alternative substrates to enhance BNC production yield, cost-effectiveness, and environmental sustainability.

The aim of the current case study is to sequentially assess the heavy metals (HMs) cadmium (Cd), iron (Fe), lead (Pb) and zinc (Zn) in five products (raw juice, bagasse, mud-cake, refined sugar and molasses) along with the effluents of the selected sugar industry (SSI) Sindh, Pakistan. Estimations of daily intake and possible toxic risk of HMs by the consumption of sugar of SSI to end-user (families of SSI workers) of age group of 2.0–5.0, 6.0–15.0, 16.0–30.0 and 31.0–50.0 years were conducted. The high contents of HMs were observed in molasses followed by mud-cake and bagasse (raw waste). The raw juice of sugarcane and refined/white sugar showed the lowest levels of these HMs. The low levels of HMs in effluents of SSI might be to their separation at different stages of sugar processing and refining into the mud-cake, bagasse and molasses. The calculated daily intake of Fe and Zn from refined/white sugar to various study groups was found to be adequate based on the recommended levels by Food and Nutrition Board (FNB). The estimated weekly intake (WI) for Cd and Pb by the consumption of SSI sugar was significantly lower (2–5 times) than the provisional acceptable WI of Cd and Pb by WHO from foodstuff. The potential toxic risk study of Cd and Pb revealed that the studied subjects were not at potential toxic risk by the consumption of SSI sugar. [ABSTRACT FROM AUTHOR]

Ectoine, a biologically significant compound, was successfully produced by a strain of bacteria capable of utilizing sucrose. In a ground-breaking approach, we harnessed the potential of sugar beet molasses, a by-product rich in sucrose, amino acid, and vitamins, as a growth medium for this purpose. Through meticulous investigation, we identified the ideal conditions for maximizing ectoine synthesis. This remarkable milestone was reached by introducing only 1 g of (NH₄)₂SO₄ and 5 mL of molasses per liter, maintaining a pH level of 8.0, upholding a 7.5% NaCl concentration, employing agitation at 120 rpm, and sustaining a temperature of 30 °C. This study marks a pioneering endeavour as it represents the first instance where molasses has been effectively employed to produce ectoine through the cultivation of Nesterenkonia sp. We showcased the production of 75.56 g of the valuable compound ectoine utilizing 1 L of waste molasses with this specific bacterial strain. These findings hold tremendous promise, not only in terms of resource utilization but also for the potential applications of ectoine in various biological contexts. [ABSTRACT FROM AUTHOR]

Molasses of foredeeps are important indicators of the newest orogenic uplifts, as well as the source of data on climate and landscape changes. One of the fullest sections of Neogene–Quaternary deposits is studied in valleys of the Belaya, Pshekha, and Psekups rivers at the junction of the Western and Northwestern Caucasus with the Eastern Kuban and Western Kuban foredeeps. The formation of the deposits corresponds to the main evolution stages of the Great Caucasus orogen, as well as the foredeeps. A summary of extensive published and original tectonostratigraphic materials has shown that the lowland and then hilly relief in an axial zone of Western Caucasus existed at least from the Middle Miocene. At the same time, the northern flank of the present-day orogen and the foredeeps were located at sea level and were repeatedly flooded by the seas up to the Kuyalnikian (Piacenzian–Gelasian) time, and the Western Kuban Foredeep was flooded even later. The main data on stratigraphy of the upper molasses and Pliocene–Quaternary tectonic movements of the region are based on facies analysis and bio- and magnetostratigraphic studies of the Upper Pliocene–Lower Pleistocene Belorechensk Formation. Its sedimentation started at the beginning of the Kuyalnikian as a result of an increase in the energy of mountain rivers due to the uplift of the riverheads. It is stated that the minimum average rate of uplift of the Western Caucasus in the basin of the Belaya River is 0.8 mm/year over last 4 mln years with acceleration up to 1.7 mm/year from the beginning of the Calabrian. The Belorechensk Formation includes three subformations, which successively become coarser clastic and correspond to the main stages of the accumulation of upper molasses in the Late Pliocene and Early Pleistocene during the intensification of uplifts and landscape-climate changes of Western Caucasus and Ciscaucasia. [ABSTRACT FROM AUTHOR]

Kombucha is a functional drink which is obtained from the symbiotic culture of acetic acid bacteria and osmophilic yeasts in sweet tea and has a sour taste. During fermentation of this product, in addition to the many metabolites that are produced, a cellulose layer is formed by Acetobacter xyleneum in the fermented liquid. This drink has many benefits like probiotic and antioxidant properties, prevention and treatment of cancer and diabetes, increment the body’s metabolism, and etc. Also, the cellulose layer produced is used to remove metal contaminants from the effluent, treat wounds and burns, and as poultry dietary supplement. In this study, optimization of kombucha bacterial cellulose using sugarcane molasses as a source of carbon and energy, and in different environmental conditions (temperature (25, 27.5, 30 °C), incubation time (7, 11, 15 days), initial pH (5.5, 6, 6.5)) analyzed using central composite statistical plan. The results showed that concentration of sugarcane molasses, incubation time, and initial pH have significant effect on production of kombucha bacterial cellulose (p<0.05). [ABSTRACT FROM AUTHOR]

Organic waste valorization is one of the principal goals of the circular economy. Bioprocesses offer a promising approach to achieve this goal by employing microorganisms to convert organic feedstocks into high value products through their metabolic activities. In this study, a fermentation process for yeast cultivation and extracellular lipase production was developed by utilizing food waste. Lipases are versatile enzymes that can be applied in a wide range of industrial fields, from detergent, leather, and biodiesel production to food and beverage manufacturing. Among several oleaginous yeast species screened, Saitozyma flava was found to exhibit the highest secreted lipase activity on pNP‐butyrate, pNP‐caproate, and pNP‐caprylate. The production medium was composed of molasses, a by‐product of the sugar industry, which provided nutrients for yeast biomass formation. At the same time, waste cooking oil was employed to induce and enhance extracellular lipase production. After 48 h of process, 20 g/L of yeast biomass and 150 mU/mgdw of lipase activity were achieved, with a productivity of 3 mU/mgdw/h. The purified lipase from S. flava showed optimal performances at temperature 28°C and pH 8.0, exhibiting a specific activity of 62 U/mg when using p‐NPC as substrate. [ABSTRACT FROM AUTHOR]

The present research studies the effect of adding sugar factory waste sugarcane molasses as an alternative to chemical inhibitory additives manufactured on concrete cast during hot weather in the summer of Iraq. The current study includes a study of the setting time for cement paste and workability for fresh concrete and some mechanical properties of hardened concrete with sugarcane molasses using percentage 0–0.3% of cement weight. The study also included studying the value of pH of concrete to investigate the effect of sugarcane molasses on reinforced concrete. The results showed that the sugarcane molasses can be used by about 0.1% of cement weight for medium projects and between 0.1 and 0.2% for large projects. It was also found that the compression strength increased by about 11.5% and the indirect tensile strength increased by about 7.5 and 7.4% for splitting and flexural test, respectively for concrete mixtures containing 0.2% of sugarcane molasses. [ABSTRACT FROM AUTHOR]

Molasses can serve as a natural adhesive for plywood and particleboard. However, several disadvantages remain, including lower dimensional stability and low bonding strength compared to other adhesives. Therefore, modifications are needed to use molasses as an adhesive for plywood. This research aims to improve bio-based molasses (MO) adhesive for plywood using citric acid (CA) adhesive. In addition, this research aims to analyze the effect of adding citric acid and to investigate the optimum hot-pressing temperature to produce the best quality plywood. In the first stage, the molasses and citric acid were combined in a ratio of 100:0, 75:25, 50:50, 25:75, 0:100 w/w%. Then, the second stage focuses on analyzing the influences of pressing temperature based on an optimum first stage. The research demonstrated that the addition of CA altered the gelation time, solid content, viscosity, and pH of the molasses adhesives. In addition, the thermal properties of molasses adhesives were changed after mixing with citric acid. These phenomena indicate changes in characteristics, such as the curing of adhesive. Overall, the characteristics of plywood showed a steady improvement as the CA ratio increased but revealed a significant decline for the 25:75 MO-CA ratio. By raising the pressing temperature from 180°C to 200°C, the quality of plywood was effectively improved. The plywood that was bonded using adhesives with a 50:50 MO-CA ratio exhibited superior mechanical properties and improved dimensional stability compared to the plywood bonded solely with MO. Furthermore, the optimal mechanical and physical properties resulted in plywood bonded with a 50:50 MO-CA ratio when subjected to a pressing temperature of 200°C. The Thermal and FTIR measurements revealed that CA established ester bonds with both the MO and wood veneers. In conclusion, the mechanical characteristics of plywood were improved, while maintaining its excellent dimensional stability. [ABSTRACT FROM AUTHOR]

The ASEAN nations play a significant role in the global sugar trade, collectively contributing approximately 10% of the world's sugar production. These countries produce over 17 million tons of sugar annually, with imports and exports amounting to 9,108,624 tons and 7,421,737 tons, respectively, while domestic consumption stands at 15,836,900 tons. Thailand holds the position of the second-largest sugar exporter globally, commanding a 60% share of exports after Brazil. Indonesia ranks as the world's third-largest sugar consumer and a significant importer. The Philippines, another sugarcane-producing country, focuses primarily on domestic consumption but aims to transition into a net sugar exporter. Southeast Asian countries represent 13% of global centrifugal raw sugar exports and 14% of global sugar imports, thereby exerting considerable influence on the global sugar trade. The annual import volume across ASEAN countries typically ranges from 5 to 6 MT, with cane sugar being the primary internationally traded sugar commodity from the region. Despite having favorable conditions, cane productivity, sugar production, and the overall status of the sugar industry in these nations face region-specific challenges. Sugarcane cultivation across ASEAN nations have been affected by climatic changes such as the El Niño phenomenon over the years. The governments of ASEAN Nations are taking initiatives to overcome this challenge. Recent economic developments, such as the establishment of the ASEAN Economic Community (AEC) and the ASEAN Free Trade Area (AFTA), alongside related reforms, have spurred these countries to adapt to emerging circumstances and enhance global competitiveness. This review highlights the current state of the sugar industry in the ASEAN countries, the challenges encountered by these nations in this sector, government initiatives to boost sugarcane production, and the way forward, particularly in light of new economic developments in the region. [ABSTRACT FROM AUTHOR]

The challenge of sustainability has emerged as a primary concern across numerous industries globally including the sugar sector. The importance of adopting sustainable practices is increasingly acknowledged on a global scale. The sugar industry occupies a unique position and has the potential to make significant contributions to various vital Sustainable Development Goals. Over the years, the sugar industry has supported and advanced these goals through diverse strategies, including the utilization of sugarcane byproducts for alternative purposes such as ethanol production, cogeneration of energy from cane bagasse, and the development of bio-based products. In this endeavor, ASEAN countries are united in harnessing the versatility of sugarcane bio-products for a greener and more resilient future. Sugarcane offers a sustainable alternative to traditional fossil-based byproducts through the production of biofuels, biochemicals, and bioplastics. This transition to a bio-based economy not only conserves limited resources by reducing usage but also diminishes the carbon footprint. By embracing and preserving these renewable resources, the various initiatives and efforts undertaken by ASEAN sugar industries can promote economic growth, environmental stewardship, and social well-being, thereby paving the way for a more sustainable and resilient future. [ABSTRACT FROM AUTHOR]

Deposition inside sugarcane factory evaporators (SFE) significantly lowers the heat transmission and can cause corrosion. This consequently reduces their efficiency, which is clearly a severe issue in the industrial sector. Up to the present time, caustic soda remains the most widely used reagent for descaling sugar industry evaporators. Understanding the scale's composition assists in determining which kinds of cleaners can effectively clean the evaporators. Scales are built in sugar evaporators as a result of the phosphitation or sulfitation processes used to chemically clean juice. In this perspective, molasses is used to descale SFE as a green manner. Molasses includes significant amounts of organic acids and nitrogenous chemical compounds, as shown by GC-mass analysis, and can be utilized as cleaning agents. XRD patterns for four scales from different sugar evaporators indicated that calcium sulfate and calcium phosphate were the predominant components at Egypt's Quos Sugarcane Factory and Dishina Sugarcane Factory, respectively. Actually, dispersed molasses demonstrated an acceptable removal effectiveness of up to 65% in all tested evaporative bodies. Furthermore, molasses solutions were tested in both basic and acidic settings and did not promote corrosion through the body's evaporative tubes. The oxidation of the molasses mixture with air or hydrogen peroxide showed that the efficacy of scale removal decreased. The findings suggested that molasses, as byproduct of sugarcane factories can be successfully employed in descaling as a green cleaning agent. This could be helpful in the development of descaling materials for the industrial sector. [ABSTRACT FROM AUTHOR]

Soy molasses, a by-product from the processing of soy protein concentrate, is a low-cost feedstock for fermentation processes due to its high content of fermentable sugars. This work investigates the use of soy molasses for growing Bacillus species, aiming at their potential application as plant growth promoters. Firstly, six Bacillus strains were screened for their ability to grow in increasing concentrations of soy molasses in a microplate assay. Following this, shaken-flask assays for growth and γ-polyglutamic acid (γ-PGA) production by three Bacillus strains in medium E and soy molasses media with 28 and 56 g L−1 of total reducing sugars (TRS) were carried out. An in vivo experiment evaluated the effect of the bacterial fermented broths on the germination and initial development of maize. Soy molasses supported the growth of Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus licheniformis in concentrations of 28 and 56 g L−1 TRS, but it was inhibitory at 112 and 224 g L−1 TRS. In soy molasses media, growth was not always associated with γ-PGA production, which was a maximum of 56 g L−1 TRS for B. amyloliquefaciens and B. licheniformis. Fermented broths with B. subtilis and B. licheniformis in soy molasses media (56 and 28 g L−1 TRS, respectively) applied to maize seeds resulted in the highest Vigor Indexes of the seedlings, which correlated negatively with the broth pH and were not impacted by the γ-PGA and indole acetic acid produced by the bacteria. The low-cost and easily available feedstock soy molasses constitutes a potential culture medium for the growth of plant growth-promoting bacteria. [ABSTRACT FROM AUTHOR]

Anaerobic digestion technology is a well-established technology for energy generation as well as an efficient and economical way of waste disposal. The research was focused on the design, development, and commissioning of a continuous stirring batch reactor to calculate the biodegradability and potential of biomethane production through biowaste (molasses). The reactor was designed using Solid Work software, and fabricated using locally available market components. The continuous stirring batch reactor (CSBR) operation was analyzed through a 30-day batch digestion experiment to estimate the methane percentage and biodegradability of the molasses at 37 °C. The maximum biomethane production was recorded as 52% in the biogas mixture produced from the anaerobic digestion of molasses with a 42% biodegradability of the feedstock. The performance of the CSBR experiment was then compared with the biogas simulator (AMPTS II) experiment using the same kind of cow dung inoculum and de-sugar molasses. The maximum biomethane potential was observed as 221L CH4 kg VS−1 produced using the bio-simulator. In contrast, a lower biomethane potential of 210L CH4 kg VS−1 was recorded using CSBR by adding VS-based 40-mL molasses. [ABSTRACT FROM AUTHOR]

The experiment aimed to investigate the impact of lactic acid bacteria, cellulase, molasses, lactic acid bacteria + cellulase, and lactic acid bacteria + molasses on the fermentation effect of the soybean-corn whole plant mixed (2∶3) wrapped silage feed in the southeastern region of Gansu. The experiment was divided into six groups, with three replicates in each group. The control group had no additives, while the additive groups were supplemented with 0.01 g/kg lactic acid bacteria, 0.005 g/kg cellulase, 50 g/kg molasses, 0.01 g/kg lactic acid bacteria + 0.005 g/kg cellulase, and 0.01 g/kg lactic acid bacteria + 50 g/kg molasses, respectively. After 60 days of aerobic storage at room temperature, the nutritional quality, fermentation quality, and aerobic stability of the mixed wrapped silage feed were tested and analyzed. The results showed that compared to the control group, the dry matter, crude protein, crude fat, phosphorus, and lactic acid content of the silage feed in the additive groups all increased, while the content of neutral detergent fiber, acid detergent fiber, pH value, and the ratio of ammonia nitrogen to total nitrogen all decreased, with the largest change observed in the lactic acid bacteria + molasses group, which had the best fermentation effect and the longest aerobic stability maintenance time, significantly different from the control group (P<0.05). The study indicates that the addition of lactic acid bacteria, cellulase, molasses, lactic acid bacteria + cellulase, and lactic acid bacteria + molasses to the soybean-corn whole plant mixed wrapped silage feed can improve the nutritional quality of the mixed silage feed, enhance the fermentation effect, and increase the aerobic stability maintenance time of the feed, with the addition of lactic acid bacteria + molasses showing the best effect. [ABSTRACT FROM AUTHOR]

Strawberry (Fragaria x ananassa) is a popular fruit consumed for its desirable flavour, aroma and colour and is considered a functional food due to its antioxidant properties. Noting the need for farmers to increase productivity to meet increasing consumer demands, this study aimed to explore the potential of biostimulants to enhance strawberry cultivation. Herein, a field trial was conducted on a commercial strawberry farm, wherein 1944 strawberry plants received fertigation supplementation with either a control solution (nutrients only) or nutrients combined with one of two biostimulant complexes (BCs). Biostimulant complex 1 (BC1) was composed of molasses, Aloe vera extract and fish-hydrolysate, and biostimulant complex 2 (BC2) was composed of potassium silicate and potassium phosphite. Results indicated that BC1 maintained total yields but increased the sellable proportion and decreased the proportion of waste. In contrast, BC2 increased total yields with both sellable yields and waste increased. No significant changes were observed for indicators of consumer preference (flavour, aroma and fruit colour), however, on-average large increases were observed to indicators of fruit antioxidant properties (phenolic and flavonoid content). Accordingly, these results support the utilisation of biostimulants as a strategy to improve strawberry on-farm productivity without compromising consumer quality measures. [ABSTRACT FROM AUTHOR]

Background: An important step in replacing petrochemical products with sustainable, cost-effective alternatives is the use of feedstocks other than, e.g., pure glucose in the fermentative production of platform chemicals. Ustilaginaceae offer the advantages of a wide substrate spectrum and naturally produce a versatile range of value-added compounds under nitrogen limitation. A promising candidate is the dicarboxylic acid malic acid, which may be applied as an acidulant in the food industry, a chelating agent in pharmaceuticals, or in biobased polymer production. However, fermentable residue streams from the food and agricultural industry with high nitrogen content, e.g., sugar beet molasses, are unsuited for processes with Ustilaginaceae, as they result in low product yields due to high biomass and low product formation. Results: This study uncovers challenges in evaluating complex feedstock applicability for microbial production processes, highlighting the role of secondary substrate limitations, internal storage molecules, and incomplete assimilation of these substrates. A microliter-scale screening method with online monitoring of microbial respiration was developed using malic acid production with Ustilago trichophora on molasses as an application example. Investigation into nitrogen, phosphate, sulphate, and magnesium limitations on a defined minimal medium demonstrated successful malic acid production under nitrogen and phosphate limitation. Furthermore, a reduction of nitrogen and phosphate in the elemental composition of U. trichophora was revealed under the respective secondary substrate limitation. These adaptive changes in combination with the intricate metabolic response hinder mathematical prediction of product formation and make the presented screening methodology for complex feedstocks imperative. In the next step, the screening was transferred to a molasses-based complex medium. It was determined that the organism assimilated only 25% and 50% of the elemental nitrogen and phosphorus present in molasses, respectively. Due to the overall low content of bioavailable phosphorus in molasses, the replacement of the state-of-the-art nitrogen limitation was shown to increase malic acid production by 65%. Conclusion: The identification of phosphate as a superior secondary substrate limitation for enhanced malic acid production opens up new opportunities for the effective utilization of molasses as a more sustainable and cost-effective substrate than, e.g., pure glucose for biobased platform chemical production. [ABSTRACT FROM AUTHOR]

Introduction: Ethanol production from sugarcane generates significant by-products, particularly vinasse, which poses environmental challenges. Objective: To evaluate the use of vinasse as a diluent in molasses fermentation to reduce water consumption and enhance the sustainability of the ethanol production process. Materials and Methods: Molasses from the “Heriberto Duquesne” Sugar Agroindustrial Company in Villa Clara, Cuba, was used, and the vinasse was extracted from the distillation column. Five treatments were tested, with vinasse dilutions between 10% to 50%. Fermentations were conducted in duplicate and monitoring the brix and pH for 40 hours. Results and Discussion: The best fermentation efficiencies were achieved with 10% and 20% vinasse, resulting in 77.67% and 77.28% efficiencies, respectively. The highest vinasse concentrations resulted in a decrease in pH and lower ethanol production. The optimal vinasse recirculation rate was determined to be 18%, reaching an efficiency of 78.1%. This recirculation reduced water consumption by approximately 20%, which is equivalent to a daily saving of 13.7 m³ of fresh water. Conclusions: The study concludes that using vinasse as a diluent in molasses fermentation can reduce water usage without negatively affecting fermentation efficiency. It is feasible to replace up to 20% of water with vinasse for more sustainable ethanol production. [ABSTRACT FROM AUTHOR]

This study investigated the effects of sumac and molasses on nutrient composition, in vitro degradability and fermentation quality of alfalfa silage. Alfalfa was ensiled in quadruplicate in vacuum jars untreated group (A) or after the following treatments: sumac group at 10% (AS), molasses group at 5% (AM), and sumac (10%) and molasses (5%) group (ASM). Silos (n = 64) were stored for 0, 21, 45 or 60 days. The results showed that dry matter (DM) contents of the AS, AM and ASM groups were statistically higher than the control group (p < 0.001). Only on the 21st day of fermentation the crude ash content of the AS group was found to be significantly higher than the other groups (p < 0.05). In vitro, DM and organic matter degradation values of the AMS group increased significantly (p < 0.001). A significant decrease in alfalfa silage's pH values was determined with sumac and molasses additives (p < 0.001). The ammonia nitrogen (NH3‐N) values of the control, AS, AM and ASM groups at Day 60 were determined as 9.08%, 7.22%, 7.00% and 6.81% respectively (p < 0.05). The water‐soluble carbohydrate (WSC) values of all groups on the 60th day were significantly decreased compared to the 0th day (p < 0.001). When the groups were evaluated within themselves, there was a statistically significant difference between the 0th and 60th day lactic acid values. The acetic acid content of the A group on the 60th day was found to be significantly higher than the other groups (p < 0.01). There was a significant decrease in propionic acid levels on Days 21, 45 and 60 compared to Day 0 of fermentation (p < 0.001). The highest butyric acid (BA) level was determined in the A group on the 21st, 45th and 60th days of fermentation (p < 0.05). In conclusion, sumac prevents proteolysis depending on its tannin content. It improves silage fermentation positively thanks to its organic acid content, while the molasses additive is effective in silage fermentation, mainly depending on the WSC level. However, it was determined that neither additive could reduce the silage pH to the appropriate value ranges due to the low doses, and they could not mainly prevent the formation of BA. [ABSTRACT FROM AUTHOR]

Pomegranate molasses is made from concentrated pomegranate juice with nothing added. Due to its nutritional value, limitation in production, and high production cost, this product may be adulterated by date syrup. This study was done to differentiate various types of pomegranate molasses and investigate the possibility of nonauthenticity detection in pomegranate molasses samples using the hyperspectral imaging (HSI) technique compared with physicochemical measurement analysis. The physicochemical properties (brix index, sucrose, acidity, ash content, pH, and formalin index) of 24 samples were measured as the reference analysis method, and it was found that the formalin index was a good factor for pomegranate molasses authenticity evaluation. Additionally, an HSI system (400–1000 nm) was used as a nondestructive and rapid screening method to capture spectral data of the samples. The evolutionary wavelength selection algorithm was applied to select effective wavelengths in sample clustering based on the obtained Davies–Bouldin index. Next, principal component analysis was used to visually interpret the spectral data of the sample when using the selected wavelengths and the whole spectra of the samples. Finally, an automatic clustering algorithm by the artificial bee colony as an unsupervised method was developed for the clustering of the authentic and nonauthentic samples. The method did not need descriptively labeled samples and obtained agreed satisfactorily with the degree of nonauthenticity in the samples. This study showed that the developed HSI technique coupled with an automatic clustering algorithm could detect date syrup nonauthenticity in pomegranate samples from the level of 5% adulteration. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the effects of additives on the nutritional value of fermented vinegar residue. Molasses, urea, and wheat bran were added to the vinegar residue fermentation system as a carbon source, nitrogen source, and a combined source of both carbon and nitrogen, respectively. Molasses was added at the following proportions: 0, 1.5, 3 and 4.5%, urea at 0, 0.5, 1.0 and 1.5%, and wheat bran at 0, 5, 10 and 15%. Fermentation was performed using five strains of fermenting bacteria in laboratory silage bags. After four weeks, the quality of vinegar residue fermentation was evaluated using sensory index, pH value, raw ash, and crude protein contents, as well as levels of ammonia-nitrogen and short-chain fatty acids (including lactic, acetic, propionic, and butyric acids). The results revealed that the addition of 3 and 4.5% molasses significantly improved the pH value, and lactic acid concentration in the vinegar residue (P < 0.05). The addition of 4.5% urea significantly increased the pH value, lactic acid concentration, ash and crude protein contents, while decreasing the acetic acid concentration in the vinegar residue (P < 0.05). The inclusion of 15% wheat bran significantly elevated the sensory index and crude protein content, but reduced the pH value, dry matter content and acetic acid concentration in the vinegar residue (P < 0.05). So, the nutritional quality of vinegar residue can be improved by incorporating carbon and nitrogen sources into the fermentation system. The addition of 15% wheat bran appears to be an optimal choice for stimulating vinegar residue fermentation, surpassing the effectiveness of utilising carbon (molasses) and nitrogen (urea) sources individually. [ABSTRACT FROM AUTHOR]

This study compared the behavior of the biomass in two fixed-film anaerobic reactors operated under equivalent organic loading rates but at different temperatures, i.e., 30 °C (RMM) and 55 °C (RMT). The reactors were fed with sugarcane vinasse and molasses (both fermented) in a simulation of sequential periods of season and off-season. The dynamics of biomass growth and retention, as well as the microbial composition, were assessed throughout 171 days of continuous operation, coupled with an additional 10-day test assessing the microbial activity in the bed region. Despite the different inoculum sources used (mesophilic granular vs. thermophilic flocculent sludge types), the biomass growth yield was identical (0.036–0.038 g VSS g−1COD) in both systems. The retention rates (higher in RMT) were regulated according to the initial amount of biomass provided in the inoculation, resulting in similar amounts of total retained biomass (46.8 vs. 43.3 g VSS in RMT and RMM) and biomass distribution patterns (30–35% in the feeding zone) at the end of the operation. Meanwhile, hydrogenotrophic methanogenesis mediated by Methanothermobacter coupled to syntrophic acetate oxidation prevailed in RMT, while the Methanosaeta-mediated acetoclastic pathway occurred in RMM. The results show that different anaerobic consortia can behave similarly in quantitative terms when subjected to equivalent organic loads, regardless of the prevailing methane-producing pathway. The community grows and reaches a balance (or a given cell activity level) defined by the amount of substrate available for conversion. In other words, while the metabolic pathway may differ, the endpoint (the amount of biomass) remains the same if operational stability is maintained. [ABSTRACT FROM AUTHOR]

In the present study, the nutritional and sensory properties of spelt cookies without wild garlic, cookies with fresh wild garlic, cookies with osmodehydrated wild garlic in sugar beet molasses, and cookies with osmodehydrated wild garlic in an aqueous solution of sucrose and salt were evaluated and compared. The tested cookie samples were characterized in terms of total antioxidative activity, the total content of phenols, flavonoids, and thiosulfates, the presence of dominant phenols, the content of betaine and dietary fiber, antioxidant activity after in vitro digestion, and sensory attributes for appearance, taste, smell, and texture. The results proved that the addition of wild garlic leaves osmodehydrated in molasses provided the cookies with the best nutritional and bioactive properties: 1.75 times higher total phenols content, 2.4 times higher total flavonoids content, 1.52 times higher total thiosulfates content, and 1.56 times higher betaine content, and a total quality increase of 54% compared to the control cookies. The cookies enriched with osmodehydrated wild garlic in molasses were rated as pleasant and acceptable, but also more complex compared to other cookies. The production of this nutritionally and sensory-improved cookie would contribute to expanding the assortment of flour confectionery products, especially for consumers who care about health and nutrition. [ABSTRACT FROM AUTHOR]

Background: Japanese quails have the potentials of being cheap source of animal protein. This study was conducted to determine the growth pattern and physiological response of Japanese quails to administration of aqueous solution of egg lime molasses mixture (ELM). Methods: ELM was prepared by placing fresh eggs in a bowl after which 1 liter of lime juice and 500 g of molasses were added and left for 10 days at temperature of 27°C and relative humidity of 61%. The entire solution was then blended together. Two hundred day old Japanese quails were assigned to five treatments (4 replicates/treatment) with forty birds in a completely randomized design (CRD). The control (T1) having no administration of ELM, T2 had an inclusion level of 10 ml, T3: 20 ml, T4: 30 ml and T5: 40 ml ELM all into 500 ml of water. Feed and water were offered ad libitum. The study was carried out for 49 days. Data were collected on growth, morphometrics and key physiology parameters. Data were subjected to descriptive statistics and Analysis of Variance using SPSS Version 22. Result: Significant difference (p<0.05) was observed only for feed conversion ratio at the starter phase, however, birds administered 20 ml ELM/500 ml of water had the best FCR. Significant differences (p<0.05) were observed for live weight and weight gain at the finisher phase. Significant differences (p<0.05) were observed in body morphometrics with birds administered 20 ml/500 ml of water having the highest values. There were significant differences (p<0.05) in rectal temperature and respiratory rate at the starter phase while at the finisher phase, significant difference (p<0.05) was only observed in rectal temperature. It can be concluded from this study that aqueous administration of egg lime molasses solution in the diet of Japanese quails has no detrimental effect on the growth pattern and physiological response. [ABSTRACT FROM AUTHOR]

Production of carotenoids by yeast fermentation is an advantaged technology due to its easy scaling and safety. Nevertheless, carotenoid production needs an economic culture medium and other efficient yeast stains. The study aims to isolate and identify a yeast strain capable of producing carotenoids using a cost-effective substrate. A new strain was identified as Rhodotorula toruloides L/24-26-1, which can produce carotenoids at different pretreated and unpretreated sugarcane molasses concentrations (40 and 80 g/L). The highest biomass concentration (18.6 ± 0.6 g/L) was reached in the culture using 80 g/L of hydrolyzed molasses. On the other hand, the carotenoid accumulation reached the maximum value using pretreated molasses at 40 g/L (715.4 ± 15.1 µg/g d.w). In this case, the β-carotene was 1.5 times higher than that on the control medium. The yeast growth in molasses was not correlated with carotenoid production. The most outstanding production of The DPPH, ABTS, and FRAP tests demonstrated the antioxidant activity of the obtained carotenogenic extracts. This research demonstrated the R. toruloides L/24-26-1 strain biotechnological potential for carotenoid compounds. The yeast produces carotenoids with antioxidant activity in an inexpensive medium, such as sulfuric acid pretreated and unpretreated molasses. [ABSTRACT FROM AUTHOR]

The production of citric acid, a vital agricultural commodity utilized across various industries such as food, beverages, pharmaceuticals, agriculture, detergents, and cosmetics, predominantly relies on microbial fermentation, with Aspergillus niger accounting for approximately 90% of global production. In this study, we aimed to optimize the key factors influencing citric acid production, with a focus on strains, fermentation techniques, and carbon sources, particularly sugarcane molasses. A. niger, sourced from the Botany department/Biotechnology laboratories at Govt. College of Science, Lahore, was employed for citric acid production. The process involved inoculum preparation through spore collection from 3 to 5 days of cultured PDA slants. The fermentation medium, comprising cane molasses with a 15% sugar concentration, was meticulously prepared and optimized for various factors, including magnesium sulfate, potassium ferrocyanide, time of addition of potassium ferrocyanide, ammonium oxalate, and calcium chloride. Our optimization results shed light on the significant impact of different factors on citric acid production. For instance, the addition of 0.4 g/L magnesium sulfate led to a maximum yield of 75%, while 2 g/L potassium ferrocyanide, added at 24 h, achieved a yield of 78%. Remarkably, ammonium oxalate, at a concentration of 10 g/L, resulted in a notable 77% yield. Conversely, the addition of calcium chloride exhibited negligible effects on citric acid production, with the control group yielding more at 78%. Our study underscores the potential for optimizing factors to enhance citric acid production by A. niger in submerged fermentation. These findings highlight the pivotal role of magnesium sulfate, potassium ferrocyanide, and ammonium oxalate in augmenting citric acid yields while emphasizing the minimal impact of calcium chloride. Ultimately, these insights contribute to advancing our understanding of microbial citric acid biosynthesis, providing valuable implications for industrial applications and future research endeavors. [ABSTRACT FROM AUTHOR]

Sugarcane is one of the most important crops in India, which meets the sweeteners requirement of people. India is one of the largest sugarcane producers in the world. Jaggery is a conventional sugar that is unrefined and often eaten all over the world. It is regarded as the healthiest sugar in the world because of its mineral and vitamin content as present in sugar cane juice. It is produced in India by the concentration of sugar cane juice, without separating molasses. The Jaggery making plants are placed in decentralized sectors in India. The efficiency of traditional plants producing jaggery is very poor because they are manufactured by local artisans. The use of multi-pan plants with a heat pump based freeze concentration system is found to be a successful approach for efficient jaggery production from the available literature in the manufacture of jaggery manufacturing plants. Due to the presence of inverted sugars and mineral salts that are hygroscopic in nature, the storage of Jaggery is difficult. The storage of jaggery in a whey coated drying cum storage bin is found to be most efficient in preserving the consistency of jaggery. The greenhouse drying of jaggery is an effective way to extract and preserve the moisture content of jaggery for a longer period of time. [ABSTRACT FROM AUTHOR]

A novel ch让osan aerogel (QACL) was prepared by free radical polymerization using alkaline lignin (SL), chitosan (CS) and polyvinyl imide (PEI) as raw materials. The morphology, structure and thermal properties of QauL adsorbent were observed and characterized by scanning electron microscopy, Fourier transform infrared spectrometer and thermogravimetric analyzer. The effects of changing reaction time, temperature, pH and adsorbent dosage on the absorption of molasses pigment were studied. The results showed that the optimal conditions were pH 7, reaction temperature 60 °C, reaction time 30 min and adsorbent dosage 1. 1 g/L, the removal rate of molasses pigment by QACL reached 94%, and the adsorption capacity was 128. 18 mg/g. [ABSTRACT FROM AUTHOR]

In Brazilian distilleries, bioethanol is mainly produced using musts obtained from sugarcane, with production restricted to the sugarcane harvest period. The present work evaluated ethanol production using fermentation musts prepared from a mixture of sorghum juice and diluted sugarcane molasses, with the aim of shortening the off-season of ethanol production. Firstly, kinetic modeling of ethanol fermentation was performed employing musts prepared solely with diluted sugarcane molasses and sweet sorghum juice. Subsequently, ethanol fermentations were carried out with musts prepared by mixing diluted sugarcane molasses and sweet sorghum juice in different proportions. The hybrid Andrews–Levenspiel model provided excellent fits to the experimental data for the fermentations performed with the individual musts. In all the fermentations with mixed musts, there was faster substrate uptake and earlier substrate exhaustion, compared to the fermentation using only diluted sugarcane molasses. The fermentation initiated with a mixture of 25% diluted molasses and 75% sweet sorghum juice resulted in around 17% higher ethanol productivity (in units of g L−1 h−1), compared to the fermentation with sugarcane molasses alone. [ABSTRACT FROM AUTHOR]

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This study investigated the effect of molasses addition on the fermentation quality, chemical composition, and bacterial community of seed pumpkin peel residue (SPPR) mixed with sunflower straw (SS) in microstorage feed. Molasses additions on a dry matter basis (DM) were divided into three groups: 0% control (CON), 1% (MA), and 2% (MB), and the raw materials underwent mixed microstorage for a period of 60 days. MA exhibited the highest content of dry matter (DM), the lowest content of neutral detergent fiber (NDF), acid detergent fiber (ADF), and ammoniacal nitrogen (NH3-N), as well as the lowest microbial diversity abundance and the highest relative abundance of lactobacilli (p < 0.05). MB demonstrated the highest crude protein (CP) content and acetic acid (AA) and propionic acid (PA) concentrations, with the lowest pH. In conclusion, the addition of molasses could enhance the quality of mixed microsilage feeds composed of seeded pumpkin peel pomace (SPPR) and sunflower straw (SS), with the optimal addition of molasses being 1% on a DM basis. [ABSTRACT FROM AUTHOR]

2-phenylethanol (2-PE) is a valuable aromatic alcohol with diverse applications in cosmetics, food, beverages, and pharmaceutical industries. Currently, 2-PE is produced either through chemical synthesis or by extraction from plant materials. However, both conventional production methods have their own limitations. Therefore, there is a need for more eco-friendly and cost-effective approaches to produce natural 2-PE. Biotechnological routes, particularly microbial fermentations, hold promise for natural 2-PE production, especially when using low-cost substrates. In this study, 2-PE was produced by de novo synthesis via the shikimate pathway, using the yeast Yarrowia lipolytica in a medium composed of sugar beet molasses (SBM) and yeast extract (YE) as carbon and nitrogen sources, respectively. A genetically engineered strain was generated, in which the SUC2 gene was transformed, expressing the invertase enzyme, enabling Y. lipolytica to efficiently utilize SBM as a cost-effective substrate. A central composite design allowed for the optimization of the concentrations of the carbon and nitrogen sources, resulting in approximately 0.71 g(2-PE)/L(culture medium). The results obtained highlight the potential of utilizing SBM as a low-cost substrate for 2-PE production, advancing biotechnological approaches in fragrance synthesis. [ABSTRACT FROM AUTHOR]

This study investigates the applicability of the Peleg model to the osmotic dehydration of various sweet potato variety samples in sugar beet molasses, addressing a notable gap in the existing literature. The osmotic dehydration was performed using an 80% sugar beet molasses solution at temperatures of 20 °C, 35 °C, and 50 °C for periods of 1, 3, and 5 h. The sample-to-solution ratio was 1:5. The objectives encompassed evaluating the Peleg equation's suitability for modeling mass transfer during osmotic dehydration and determining equilibrium water and solid contents at various temperatures. With its modified equation, the Peleg model accurately described water loss and solid gain dynamics during osmotic treatment, as evidenced by a high coefficient of determination value (r2) ranging from 0.990 to 1.000. Analysis of Peleg constants revealed temperature and concentration dependencies, aligning with previous observations. The Guggenheim, Anderson, and de Boer (GAB) model was employed to characterize sorption isotherms, yielding coefficients comparable to prior studies. Effective moisture diffusivity and activation energy calculations further elucidated the drying kinetics, with effective moisture diffusivity values ranging from 1.85 × 10−8 to 4.83 × 10−8 m2/s and activation energy between 7.096 and 16.652 kJ/mol. These findings contribute to understanding the complex kinetics of osmotic dehydration and provide insights into the modeling and optimization of dehydration processes for sweet potato samples, with implications for food processing and preservation methodologies. [ABSTRACT FROM AUTHOR]

The world faces significant challenges in managing plastic waste, which does not decompose and releases toxic gases when burned, posing severe health hazards. Concurrently, Nepal's heavy burnt-clay brick walls in reinforced concrete building structures urgently need replacement with lightweight walls. To tackle these dual challenges, this paper uniquely explores the innovative reuse of post-consumer plastic waste aggregate (PWA) as a sustainable alternative to sand, aiming to produce lightweight mortar for enhanced building applications. The control mortar used a water: cement: sand ratio of 1:2:4 (by weight). Shredded PWA was incorporated at varying contents of 0.00 %, 2.50 %, 5.00 %, 7.50 %, and 10.00 % by weight of sand. The study also examined the effect of adding 0.05 % molasses by weight of cement on the mortar's properties. The physical, mechanical, and water absorption properties of each mortar mix were evaluated using standard tests. The table flow value and water absorption of the mortar with 10.00 % PWA content increased by 20.83 % and 148.72 %, respectively, compared to the control mortar. However, the 28-day compressive, flexural, and splitting tensile strengths decreased by 92.29 %, 41.70 %, and 83.26 %, respectively. It was feasible to use 7.50 % and 10.00 % PWA to produce lightweight panels for walls, with and without molasses, respectively. The use of PWA in concrete addresses critical plastic waste management challenges while enabling the production of sustainable structural and non-structural concrete. PWA mortar can replace heavy brick walls, reducing building weight and enhancing seismic resistance. Additionally, substituting brick walls lowers reliance on brick kilns, leading to reduced CO₂ emissions and land degradation, while also cutting concrete production costs.