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Background: The microbiomes on the surface of unclean removable prostheses are complex and yet largely underexplored using metagenomic sequencing technology. Objectives: To characterize the microbiome of removable prostheses with different levels of cleanliness using Type IIB Restriction-site Associated DNA for Microbiome (2bRAD-M) sequencing and compare the Microbial Index of Pathogenic Bacteria (MIP) between clean and unclean prostheses. Materials and Methods: Ninety-seven removable prostheses were classified into 'clean' and 'unclean' groups. All prosthesis plaque samples underwent 2bRAD metagenomic sequencing to characterize the species-resolved microbial composition. MIPs for clean and unclean prostheses were calculated based on the sum of the relative abundance of pathogenic bacteria in a microbiome using a reference database that contains opportunistic pathogenic bacteria and disease-associated information. Results: Beta diversity analyses based on Jaccard qualitative and Bray-Curtis quantitative distance matrices identified significant differences between the two groups (p < 0.05). There was a significant enrichment of many pathogenic bacteria in the unclean prosthesis group. The MIP for unclean prostheses (0.47 ± 0.25) was significantly higher than for clean prostheses (0.37 ± 0.29), p = 0.029. Conclusions: The microbial community of plaque samples from 'unclean' prostheses demonstrated compositional differences compared with 'clean' prostheses. In addition, the pathogenic microbiome in the 'unclean' versus 'clean' group differed. KEY MESSAGES: The pathogenic microbiome in the unclean removable prosthesis group tends to be more abundant than that of the clean counterpart among participants with the majority being elders attending a teaching hospital. This finding is worrying because a general decline in systemic health among community-dwelling elders may predispose them to life-threatening diseases. By understanding the characteristics of the microbiome of removable prostheses with different levels of cleanliness and the related microbial-infection risks after a comprehensive whole metagenomic sequencing, appropriate prosthesis hygiene care should be emphasized. This study introduced a comprehensive and novel method of microbiological investigation of plaque using 2bRAD-M. The taxonomic profile of the microbiome of whole genomes was demonstrated and resolved at the species-level taxonomy for prosthesis biofilms. The biggest advantage of this method included overcoming the DNA sample problems particularly the low-biomass nature of 'clean' prosthesis plaque samples. The findings will add to our understanding of the microbiological aspect of removable prosthesis plaque. [ABSTRACT FROM AUTHOR]

[Objective] To reveal the vertical distribution characteristics of soil organic carbon? nitrogen? and phosphorus content, soil stoichiometric characteristics (C/N, C/1y, N/P), enzyme activity, microorganisms, and their seasonal responses of forest stand types in Liziping Nature Reserve. _Methods］ The soils of Cyclobalanopsis and populus szechuanica broad-leaved mixed forest, Oriental oak deciduous broad-leaved forest, Fir-spruce coniferous mixed forest, and Yushania lineolata forest in the dry and rainy seasons were selected as the study objects to elucidate the effects of forest stand types on the stoichiometric characteristics of the soils, the enzyme activities, the microorganisms, and their seasonal dynamic response characteristics. Results] (1) The soil physicochemical properties and enzyme activity of the four forest stand types are higher in the rainy season than in the dry season, (2) The soil C/N in the Cyclobalanopsis-populus szechuanica broad-leaved mixed forest is higher in the dry season than in the rainy season, while the other three stands show the opposite trend. Dry season's C/1y are higher than the rainy season in Cyclobalanopsis and populus szechuanica broad-leaved mixed forest and Fir-spruce coniferous mixed forest, while the other two stands are the opposite. Dry season's N/P are higher than the rainy season in Oriental oak deciduous broad-leaved forest and Fir-spruce coniferous mixed forest, while the other two stands are the opposite. (3) Different forest types' value variation of soil C/N, C/I\and N/P are 17.0()〜37.33, 62.72〜114.5(), 3.09〜 5.18 in the dry season, and 17.72〜39.16, 63.21 〜9().72, 2.39〜5.8() in the rainy season. (4) The C/N and C/1y values during the dry and rainy seasons are higher than the national average (11.90, 61.00), indicating that nitrogen is limited during nutrient cycling and the potential for releasing I〉is low； The N/P values for both seasons were lower than the national average soil level (5.20)，indicating that the soil is more susceptible to N limitation； (5) The soil microbial activity of the four forest stand types is better in the rainy season than in the dry season, and the soil ecological stoichiometric characteristics are mainly influenced by enzyme activity and microorganisms. Conclusion] The results of this study can provide important theoretical basis for exploring the seasonal response mechanisms of regional soil nutrient decomposition, supply and limitation, as well as nutrient cycling processes. [ABSTRACT FROM AUTHOR]

Agricultural drainage water is a significant source of return water due to its decentralization, large volume, and poor quality. This study examined the effectiveness of constructed wetlands in improving sugarcane field's drainage water in the south of Khuzestan province of Iran, located approximately 17 km north of Khorramshahr city at latitude 303,805.94′ N and longitude 480,759.86′ E, by removing agricultural herbicides (atrazine, glyphosate, paraquat, and 2, 4-D) and Total Coliforms (TC) and Fecal Coliforms (FC) from April 2019 to March 2020. A reed length was divided into three equal parts at three consecutive stations, ST1, ST2, and ST3. Natural reeds had a dimension of 3.5 km long, 1.2 km wide, and 0.5 m deep between two consecutive stations. According to the study, natural reeds were effective in removing agricultural herbicides and improving agricultural drainage water quality. ST3, which was the furthest from the entry point, had the largest difference in agricultural herbicides concentrations compared to their initial concentrations, as well as the highest removal efficiency (100%) during all four retention times (1.26, 1.10, 1.30, and 1.60 days). Natural reed at this distance from the entry point significantly reduced TC and FC. The ST3 station had the highest removal efficiency of TC and FC, with average values of 62 and 83%, respectively, while ST1 had the lowest (with values of 9 and 34%). Due to the differences in performance between ST1, ST2 and ST3 stations, ST2 is considered the most efficient and cost-effective station. The Khuzestan province has a water shortage and agricultural drainage water problems. Treating drainage water with environmentally friendly methods, like natural reeds, will prevent contamination of water sources and result in a significant amount of water entering the system. [ABSTRACT FROM AUTHOR]

Background The microbiomes on the surface of unclean removable prostheses are complex and yet largely underexplored using metagenomic sequencing technology.Objectives To characterize the microbiome of removable prostheses with different levels of cleanliness using Type IIB Restriction-site Associated DNA for Microbiome (2bRAD-M) sequencing and compare the Microbial Index of Pathogenic Bacteria (MIP) between clean and unclean prostheses.Materials and Methods Ninety-seven removable prostheses were classified into ‘clean’ and ‘unclean’ groups. All prosthesis plaque samples underwent 2bRAD metagenomic sequencing to characterize the species-resolved microbial composition. MIPs for clean and unclean prostheses were calculated based on the sum of the relative abundance of pathogenic bacteria in a microbiome using a reference database that contains opportunistic pathogenic bacteria and disease-associated information.Results Beta diversity analyses based on Jaccard qualitative and Bray-Curtis quantitative distance matrices identified significant differences between the two groups (p

Removable denture wearers are at an increased risk of developing periodontal diseases due to biofilm deposition and microbial colonization on the denture surface. This study aimed to characterize and compare the metagenomic composition of saliva in denture wearers with different periodontal statuses. Twenty-four community-dwelling elders were recruited and grouped into denture wearers with active periodontitis (APD), non-denture wearers with active periodontitis (APXD), denture wearers with stable periodontal health conditions (SPCD), and non-denture wearers with stable periodontal health conditions (SPCXD). Saliva samples were collected and underwent Type IIB restriction-site-associated DNA for microbiome (2bRAD-M) metagenomic sequencing to characterize the species-resolved microbial composition. Alpha diversity analysis based on the Shannon index revealed no significant difference between groups. Beta diversity analysis using the Jaccard distance matrix was nearly significantly different between denture-wearing and non-denture-wearing groups (p = 0.075). Some respiratory pathogens, including Streptococcus agalactiae and Streptococcus pneumoniae, were detected as the top 30 species in saliva samples. Additionally, LEfSe analysis revealed a substantial presence of pathogenic bacteria in denture groups. In the cohort of saliva samples collected from community-dwelling elders, a remarkable abundance of certain opportunistic pathogens was detected in the microbial community. [ABSTRACT FROM AUTHOR]

Copyright of China Condiment is the property of China Condiment and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Removable denture wearers are at an increased risk of developing periodontal diseases due to biofilm deposition and microbial colonization on the denture surface. This study aimed to characterize and compare the metagenomic composition of saliva in denture wearers with different periodontal statuses. Twenty-four community-dwelling elders were recruited and grouped into denture wearers with active periodontitis (APD), non-denture wearers with active periodontitis (APXD), denture wearers with stable periodontal health conditions (SPCD), and non-denture wearers with stable periodontal health conditions (SPCXD). Saliva samples were collected and underwent Type IIB restriction-site-associated DNA for microbiome (2bRAD-M) metagenomic sequencing to characterize the species-resolved microbial composition. Alpha diversity analysis based on the Shannon index revealed no significant difference between groups. Beta diversity analysis using the Jaccard distance matrix was nearly significantly different between denture-wearing and non-denture-wearing groups (p = 0.075). Some respiratory pathogens, including Streptococcus agalactiae and Streptococcus pneumoniae, were detected as the top 30 species in saliva samples. Additionally, LEfSe analysis revealed a substantial presence of pathogenic bacteria in denture groups. In the cohort of saliva samples collected from community-dwelling elders, a remarkable abundance of certain opportunistic pathogens was detected in the microbial community.

Tillage has been reported to induce seasonal changes of organic carbon (Cmicro) and nitrogen (Nmicro) in the biomass of microorganisms. Soil microorganisms execute such ecosystem functions as it is an immediate sink of labile biophil elements; it is an agent of a conversion, catalysis and synthesis of humus substances; it transforms soil contaminants into nonhazardous wastes and it participates in soil aggregation and pedogenesis as a whole. However, the seasonal turnover of microorganisms on arable lands in temperate ecosystems has not been studied at a relevant level. Hence, we are aimed at studying the dynamics of such soil microbial biomass patterns as Cmicro, Nmicro, microbial index (MI = (Cmicro/CTOC)·100%) and CO2-C emissions against the background of 9 years of tillage and 22 years of abandoned (Ab) and fallow (F) usage. Our study was conducted on a long-term experimental site on a Mollisol in Northeast China. The maximum Cmicro and Nmicro contents were recorded at the beginning of the growing season at the 0–10-cm layer and mid-July at the 20–40-cm layer, while the minimum content was during August–October. The Cmicro content ranged from 577.79 to 381.79 mg−1 kg−1 using Ab in the spring to 229.53 to 272.86 mg−1 kg−1 in the autumn using CT (conventional tillage) and F in the 0–10- and 10–20-cm layers, respectively. The amplitude of Nmicro content changes were several times lower as compared with the Cmicro. The smallest quartile range (IQR0.25–0.75) of such changes was shown when using the following treatments: no till (NT) and Ab in the 0–10-, NT and F in the 10–20- and CT in the 20–40-cm layer. The widest Cmicro:Nmicro ratio was recorded at F and CT in the 0–20- and CT and rotational tillage (Rot) in the 20–40-cm layer. The MI dynamics were similar to the trends of Cmicro and Nmicro and changed from 0.72 ± 0.168 to 2.00 ± 0.030%. The highest share of Cmicro in CTOC was at Ab (1.82 ± 1.85%) and NT (1.66 ± 1.52 %) in the 0–10-, Ab (1.23 ± 1.27%) and NT (1.29 ± 1.32%) in the 10–20- and Ab (1.19 ± 1.09%) and F (1.11 ± 1.077%) in the 20–40-cm layer, correspondingly. The Pearson's correlation coefficient between Cmicro and CTOC increased from the upper 0–10- to the lower 20–40-cm layer; it was "strong" and "high" between Cmicro and CTOC. Different uses of Mollisol affected the amplitude of the Cmicro and Nmicro seasonal changes, but it did not change their trend. Our results suggest the key role of Ab and NT technologies in Cmicro accumulation in the total organic carbon (TOC). [ABSTRACT FROM AUTHOR]

The purpose of this study was to establish reference ranges for gut microbial indices by collecting real-world Japanese microbiome data from a Mykinso cohort. Although several large cohort studies have focused on the human gut microbiome, large cohort studies of the gut microbiome from Japanese populations are scarce, especially from healthy or non-diseased individuals. We collected stool samples and original survey lifestyle information from 5,843 Japanese individuals through the Mykinso gut microbiome testing service. From the obtained 16S rRNA sequence data derived from stool samples, the ratio and distribution of each taxon were analyzed. The relationship between different epidemiological attributes and gut microbial indicators were statistically analyzed. The qualitative and quantitative indicators of these common gut microbiota were confirmed to be strongly correlated with age, sex, constipation/diarrhea, and history of lifestyle-related diseases. Therefore, we set up a healthy sub-cohort that controlled for these attribute factors and defined reference ranges from the distribution of gut microbial index in that population. Taken together, these results show that the gut microbiota of Japanese people had high beta-diversity, with no single "typical" gut microbiota type. We believe that the reference ranges for the gut microbial indices obtained in this study can be new reference values for determining the balance and health of the gut microbiota of an individual. In the future, it is necessary to clarify the clinical validity of these reference values by comparing them with a clinical disease cohort. [ABSTRACT FROM AUTHOR]

Tillage has been reported to induce seasonal changes of organic carbon (Cmicro) and nitrogen (Nmicro) in the biomass of microorganisms. Soil microorganisms execute such ecosystem functions as it is an immediate sink of labile biophil elements; it is an agent of a conversion, catalysis and synthesis of humus substances; it transforms soil contaminants into nonhazardous wastes and it participates in soil aggregation and pedogenesis as a whole. However, the seasonal turnover of microorganisms on arable lands in temperate ecosystems has not been studied at a relevant level. Hence, we are aimed at studying the dynamics of such soil microbial biomass patterns as Cmicro, Nmicro, microbial index (MI = (Cmicro/CTOC)·100%) and CO2-C emissions against the background of 9 years of tillage and 22 years of abandoned (Ab) and fallow (F) usage. Our study was conducted on a long-term experimental site on a Mollisol in Northeast China. The maximum Cmicro and Nmicro contents were recorded at the beginning of the growing season at the 0–10-cm layer and mid-July at the 20–40-cm layer, while the minimum content was during August–October. The Cmicro content ranged from 577.79 to 381.79 mg−1 kg−1 using Ab in the spring to 229.53 to 272.86 mg−1 kg−1 in the autumn using CT (conventional tillage) and F in the 0–10- and 10–20-cm layers, respectively. The amplitude of Nmicro content changes were several times lower as compared with the Cmicro. The smallest quartile range (IQR0.25–0.75) of such changes was shown when using the following treatments: no till (NT) and Ab in the 0–10-, NT and F in the 10–20- and CT in the 20–40-cm layer. The widest Cmicro:Nmicro ratio was recorded at F and CT in the 0–20- and CT and rotational tillage (Rot) in the 20–40-cm layer. The MI dynamics were similar to the trends of Cmicro and Nmicro and changed from 0.72 ± 0.168 to 2.00 ± 0.030%. The highest share of Cmicro in CTOC was at Ab (1.82 ± 1.85%) and NT (1.66 ± 1.52 %) in the 0–10-, Ab (1.23 ± 1.27%) and NT (1.29 ± 1.32%) in the 10–20- and Ab (1.19 ± 1.09%) and F (1.11 ± 1.077%) in the 20–40-cm layer, correspondingly. The Pearson’s correlation coefficient between Cmicro and CTOC increased from the upper 0–10- to the lower 20–40-cm layer; it was “strong” and “high” between Cmicro and CTOC. Different uses of Mollisol affected the amplitude of the Cmicro and Nmicro seasonal changes, but it did not change their trend. Our results suggest the key role of Ab and NT technologies in Cmicro accumulation in the total organic carbon (TOC).

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The purpose of this research was to (1) identify an existing water quality index methodology that could be used in Abu Dhabi, United Arab Emirates, and (2) apply the water quality index to Abu Dhabi's waters to communicate water quality conditions and track changes in conditions over time. The available monitoring data were reviewed to establish the types and frequencies of data available to populate an index, and a literature search was performed to identify and review existing water quality ind in use around the world. The Canadian Council of Ministers of the Environment Water Quality Index was selected and customized for use in Abu Dhabi. Using the Canadian Council of Ministers Water Quality Index, two different data aggregation methods were investigated for developing a single Emirate-wide Water Quality Index score. Finally, an alternative method for calculating indices when there are fewer than four parameters was investigated. Three Water Quality Indexs were developed for use in Abu Dhabi: Eutrophication Index, Microbial Index, and Heavy Metals (in sediment) Index. The Canadian Council of Ministers Water Quality Index methodology was found to be relatively easy to use and flexible as a building block for tailored indices, which are used to communicate marine water quality conditions to various stakeholders, including scientists, managers, policymakers, and the public. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Food Safety & Quality is the property of Journal of Food Safety & Quality Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Microbial indexes of filamentous bacterial density were evaluated for their potential to act as indicators of sludge settling characteristics for sequencing batch reactor (SBR) and membrane bioreactor (MBR) treatment options. These options were operated using settled sewage over a range of aerated loading rates from 0.05 to 0.4/d and the evolution of protozoan and metazoan populations analysed. A filament density score ranging between 0 and 5, which has previously been applied to conventional activated sludge processes, was shown also to be a useful tool for the SBR and MBR, and was correlated to the settleability of the mixed liquor from both reactors. Due to the hydrodynamics of both systems and the subsequent differences in mixed liquors, optimum performance for each bioreactor was obtained under different operating conditions. Although there was no correlation between the numbers of any given protozoan species and plant operating conditions, there was a clear dependence between operating conditions and protozoan diversity. The highest diversity was found when operating conditions were optimum for both the SBR and MBR. [ABSTRACT FROM PUBLISHER]

Abstract: Severe soil and water loss has led to widespread land degradation of the Loess Plateau, China. During the past two decades, significant efforts have been made to revegetate degraded soil in the region. However, a better understanding of the ability of plants to promote soil microbial processes is important for successful revegetation. The current study was conducted at the Dunshan watershed on the Loess Plateau, with the aim of evaluating the effect of different vegetation types on the microbial properties of rhizosphere soil. Six vegetation types were evaluated: two natural grassland (Artemisia capillaries and Heteropappus altaicus), two artificial (i.e. planted by humans) grassland (Astragalus adsurgens and Panicum virgatum) and two artificial shrubland (Caragana korshinskii and Hippophae rhamnoides) species; an area without vegetation cover was used as a control. The results indicated that the highest values of soil organic C, total nitrogen (TN), microbial biomass carbon (MBC), MBN, saccharase, urease, catalase and peroxidase were recorded for H. altaicus and A. capillaries; of basal respiration (BR), the MBN: TN ratio, alkaline phosphatase and polyphenol oxidase for A. adsurgens; and of the BR: MBC ratio for P. virgatum. A rhizosphere soil microbial index (RSMI) was obtained using principal component analysis, and metabolic quotient (BR/MBC), BR, urease, water-soluble C and cellulase were found to be most important for assessing rhizosphere soil quality. All revegetated soil showed higher RSMI compared with control soil, with soil under natural grassland species (H. altaicus and A. capillaries) recording the highest value. It was concluded that plant species under arid climatic conditions have different effects on the microbial properties of rhizosphere soil owing to their different root exudates and plant debris. In addition, natural grasslands are most effective for revegetation of the Loess Plateau. [Copyright &y& Elsevier]

Abstract: Microbial characterization of the tree rhizosphere provides important information relating to the screening of tree species for re-vegetation of degraded land. Rhizosphere soil samples collected from a few predominant tree species growing in the coal mining ecosystem of Dhanbad, India, were analyzed for soil organic carbon (SOC), mineralizable N, microbial biomass carbon (MBC), active microbial biomass carbon (AMBC), basal soil respiration (BSR), and soil enzyme activities (dehydrogenase, urease, catalase, phenol oxidase, and peroxidase). Among the tree species studied, Aegle marmelos recorded the highest value for MBC (590mg kg−1), urease (190.5μg NH4 +-N g−1 h−1), catalase (513μg H2O2 g−1 h−1), dehydrogenase (92.3μg TPF g−1 h−1), phenol oxidase (0.057μM g−1 h−1) and BSR/AMBC (0.498mg CO2-C mg biomass−1 day−1); Tamarindus indica for mineralizable N (69.5mg kg−1); Morus alba for catalase (513μg H2O2 g−1 h−1) and phenol oxidase (0.058μM g−1 h−1); Tectona grandis for peroxidase (0.276μM g−1 h−1), AMBC/MBC (99.4%), and BSR/MBC (0.108mg CO2-C mg biomass−1 day−1); Ficus religiosa for AMBC (128.4mg kg−1) and BSR (12.85mg CO2-C kg−1 day−1); Eugenia jambolana for MBC/SOC (8.03%); Butea monosoperma for AMBC/SOC (1.32%) and Azadirachta indica for BSR/AMBC (0.1134mg CO2-C mg biomass−1 day−1). Principal component analysis was employed to derive a rhizosphere soil microbial index (RSMI) and accordingly, dehydrogenase, BSR/MBC, MBC/SOC, EC, phenol oxidase and AMBC were found to be the most critical properties. The observed values for the above properties were converted into a unitless score (0–1.00) and the scores were integrated into RSMI. The tree species could be arranged in decreasing order of the RSMI as: A. marmelos (0.718), A. indica (0.715), Bauhinia bauhinia (0.693), B. monosperma (0.611), E. jambolana (0.601), Moringa oleifera (0.565), Dalbergia sissoo (0.498), T. indica (0.488), Morus alba (0.415), F. religiosa (0.291), Eucalyptus sp. (0.232) and T. grandis (0.181). It was concluded that tree species in coal mining areas had diverse effects on their respective rhizosphere microbial processes, which could directly or indirectly determine the survival and performance of the planted tree species in degraded coal mining areas. Tree species with higher RSMI values could be recommended for re-vegetation of degraded coal mining area. [Copyright &y& Elsevier]

The purpose of this study was to establish reference ranges for gut microbial indices by collecting real-world Japanese microbiome data from a Mykinso cohort. Although several large cohort studies have focused on the human gut microbiome, large cohort studies of the gut microbiome from Japanese populations are scarce, especially from healthy or non-diseased individuals. We collected stool samples and original survey lifestyle information from 5,843 Japanese individuals through the Mykinso gut microbiome testing service. From the obtained 16S rRNA sequence data derived from stool samples, the ratio and distribution of each taxon were analyzed. The relationship between different epidemiological attributes and gut microbial indicators were statistically analyzed. The qualitative and quantitative indicators of these common gut microbiota were confirmed to be strongly correlated with age, sex, constipation/diarrhea, and history of lifestyle-related diseases. Therefore, we set up a healthy sub-cohort that controlled for these attribute factors and defined reference ranges from the distribution of gut microbial index in that population. Taken together, these results show that the gut microbiota of Japanese people had high beta-diversity, with no single "typical" gut microbiota type. We believe that the reference ranges for the gut microbial indices obtained in this study can be new reference values for determining the balance and health of the gut microbiota of an individual. In the future, it is necessary to clarify the clinical validity of these reference values by comparing them with a clinical disease cohort., (©2021 BMFH Press.)