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201 results on '"michelet, Lorraine"'

6. Experimental infection of goats with Mycobacterium microti induces subclinical pulmonary tuberculosis and mild responses to tuberculin skin tests

Author

Melgarejo, Cristian, primary, Cobos, Alex, additional, Domingo, Mariano, additional, Cantero, Guillermo, additional, Moll, Xavier, additional, Sevilla, Iker A., additional, Garrido, Joseba M., additional, Michelet, Lorraine, additional, Boschiroli, Maria Laura, additional, Vidal, Enric, additional, and de Val, Bernat Pérez, additional

Published
2024
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8. Mycobacterium microti Infection in Free-Ranging Wild Boar, Spain, 2017-2019

Author

de Val, Bernat Perez, Sanz, Albert, Soler, Merce, Allepuz, Alberto, Michelet, Lorraine, Boschiroli, Maria Laura, and Vidal, Enric

Subjects
Health aspects, Infection -- Health aspects, Tuberculosis -- Health aspects, Pets, Beef cattle, Rodents, Wildlife
Abstract

A Mycobacterium microti is a member of the Mycobacterium tuberculosis complex (MTBC), which also includes M. tuberculosis and M. bovis, the main causes of human and animal tuberculosis (TB), respectively. [...]

Published
2019
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9. Phylogenomic Perspective on a Unique Mycobacterium bovis Clade Dominating Bovine Tuberculosis Infections among Cattle and Buffalos in Northern Brazil

Author

Conceição, Marília Lima da, Conceição, Emilyn Costa, Furlaneto, Ismari Perini, Silva, Sandro Patroca da, Guimarães, Arthur Emil dos Santos, Gomes, Pedro, Boschiroli, María Laura, Michelet, Lorraine, Kohl, Thomas Andreas, Kranzer, Katharina, Francez, Loreno da Costa, Lima, Luana Nepomuceno Gondim Costa, Portugal, Isabel, Perdigão, João, and Lima, Karla Valéria Batista

Published
2020
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10. Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria

Author

Fernández Veiga, Leire, Fuertes, Miguel, Geijo, María V., Pérez de Val, Bernat, Vidal, Enric, Michelet, Lorraine, Boschiroli, María Laura, Gómez Buendía, Alberto, Bezos Garrido, Javier, Jones, Gareth J., Vordermeier, Martin, Juste, Ramón A., Garrido, Joseba M., Sevilla, Iker A., Fernández Veiga, Leire, Fuertes, Miguel, Geijo, María V., Pérez de Val, Bernat, Vidal, Enric, Michelet, Lorraine, Boschiroli, María Laura, Gómez Buendía, Alberto, Bezos Garrido, Javier, Jones, Gareth J., Vordermeier, Martin, Juste, Ramón A., Garrido, Joseba M., and Sevilla, Iker A.

Abstract

Author contributions J.M.G. and I.A.S. conceived and planned the study and obtained the funding. B.P.V., L.M., M.L.B., J.B., G.J.J., H.M.V., J.M.G. and I.A.S. contributed to choosing isolates and/or deciding skin test protocols and antigens. B.P.V., L.M. and M.L.B. provided isolates. G.J.J. and H.M.V. provided antigens. L.F.V., M.F., M.V.G., J.M.G. and I.A.S. performed the experiments and laboratory analysis and compiled the data. R.A.J., J.M.G. and I.A.S. analyzed and interpreted the results. R.A.J., J.M.G. and I.A.S. wrote the original draf of the manuscript. All authors reviewed the original draft and contributed to writing and editing the final version of the manuscript. Additional information Correspondence and requests for materials should be addressed to I.A.S., The single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than Mycobacterium tuberculosis complex (MTC) can result in nonspecific reactions to these tests. We evaluated the performance of the skin test with PPDs and new defined antigens in the guinea pig model. A standard dose (SD) of Rhodococcus equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis, M. avium subsp. avium, M. avium subsp. hominissuis, M. scrofulaceum, M. persicum, M. microti, M. caprae and M. bovis, and a higher dose (HD) of M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis were tested using PPD-B, PPD-A, P22, ESAT-6-CFP-10-Rv3615c peptide cocktail long (PCL) and fusion protein (FP). The SD of R. equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare and M. avium subsp. paratuberculosis did not cause any reactions. The HD of M. nonchromogenicum, M. monacense, M. intracellulare, and M. avium subsp. paratuberculosis and the SD of M. avium subsp. hominissuis, M. scrofulaceum and M. persicum, caused nonspecific reactions (SIT). A CITT interpretation would have considered M. avium complex and M. scrofulaceum groups negative, but not all individuals from M. nonchromogenicum HD, M. monacense HD and M. persicum SD groups. Only animals exposed to M. bovis and M. caprae reacted to PCL and FP. These results support the advantage of complementing or replacing PPD-B to improve specificity without losing sensitivity., Ministerio de Ciencia, Innovación y Universidades, INTERREG POCTEFA, European Regional Development Fund, Depto. de Sanidad Animal, Centro de Vigilancia Sanitaria Veterinaria (VISAVET), TRUE, pub

Published
2023

11. Genetic Diversity and Population Structure of Mycobacterium bovis at the Human-Animal-Ecosystem Interface in France: “A One Health approach”

Author

Appegren, Anaïs, primary, Boschiroli, Maria Laura, additional, Cruz, Krystel De, additional, Michelet, Lorraine, additional, Héry-Arnaud, Geneviève, additional, Kempf, Marie, additional, Lanotte, Philippe, additional, Bemer, Pascale, additional, Peuchant, Olivia, additional, Pestel-Caron, Martine, additional, Skalli, Soumaya, additional, Brasme, Lucien, additional, Martin, Christian, additional, Enault, Cecilia, additional, Carricajo, Anne, additional, Guet-Revillet, Hélène, additional, Ponsoda, Michaël, additional, Jacomo, Véronique, additional, Bourgoin, Anne, additional, Trombert-Paolantoni, Sabine, additional, Carrière, Christian, additional, Dupont, Chloé, additional, Conquet, Guilhem, additional, Galal, Lokman, additional, Banuls, Anne-Laure, additional, and Godreuil, Sylvain, additional

Published
2023
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13. Mycobacterium bovis Infection of Red Fox, France

Author

Michelet, Lorraine, De Cruz, Krystel, Henault, Sylvie, Tambosco, Jennifer, Richomme, Celine, Reveillaud, Edouard, Gares, Helene, Moyen, Jean-Louis, and Boschiroli, Maria Laura

Subjects
Distribution, Models, Health aspects, Company distribution practices, Red fox -- Health aspects, Epidemics -- Models -- France, Medical research, Mycobacterium infections -- Distribution
Abstract

A Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex (MTBC), is the main etiologic agent of bovine tuberculosis (TB). In France and other countries in Europe, this ancient zoonotic [...]

Published
2018
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18. ASMOHEJP2020 poster

Author

CHARLES, Ciriac, MICHELET, Lorraine, CONDE, Cyril, BRANGER, Maxime, COCHARD, Thierry, BIET, Franck, and BOSCHIROLI, Maria Laura

Abstract

ASMOHEJP 2020 POSTER PRESENTATION PRAGUE (CZECH REPUBLIC) &nbsp

Published
2022
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20. New Mycobacterium bovis complete genomes of different clonal complexes to improve molecular epidemiology of french field strains

Author

Charles, Ciriac, Michelet, Lorraine, Vorimore, Fabien, Conde, Cyril, Cochard, Thierry, Biet, Franck, Boschiroli, Maria‐laura, Unité Zoonoses Bactériennes (UZB), Laboratoire de santé animale, sites de Maisons-Alfort et de Normandie, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Infectiologie et Santé Publique (UMR ISP), Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Paris-Est (UPE), Laboratoire de sécurité des aliments de Maisons-Alfort (LSAl), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), One health EJP, and European Project: 773830, H2020-SFS-2017-1 ,One Health EJP(2018)

Subjects
mycobacterium bovis, lineages, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], genome
Abstract

International audience; Bovine Tuberculosis (bTB) is a zoonotic disease due to Mycobacterium bovis (M. bovis). France has a bTB-free status, but the disease has not been eradicated yet and a worryingly steady increase of bTB outbreaks has been observed in some regions (1). This could be explained by the detection of bTB in wildlife that spills it back to livestock in the same territories. The transmission link within these multi-host systems remains difficult to establish given that they share the same M. bovis genotypes (2). Obtaining new reference genomes for each of these genotypes could improve the knowledge of clonal groups and refine molecular field epidemiology studies based on whole genome sequencing.

Published
2022

21. Experimental Mycobacterium microti Infection in Bank Voles (Myodes glareolus)

Author

Vidal Barba, Enric, Burgaya, Judit, Michelet, Lorraine, Arrieta-Villegas, Claudia, Cantero, Guillermo, de Cruz, Krystel, Tambosco, Jennifer, Di Bari, Michelle, Romolo, Nonno, Boschiroli, Maria Laura, Pérez de Val, Bernat, Vidal Barba, Enric, Burgaya, Judit, Michelet, Lorraine, Arrieta-Villegas, Claudia, Cantero, Guillermo, de Cruz, Krystel, Tambosco, Jennifer, Di Bari, Michelle, Romolo, Nonno, Boschiroli, Maria Laura, and Pérez de Val, Bernat

Abstract

Voles are maintenance hosts of Mycobacterium microti. In line with the goal to eradicate tuberculosis (TB) in livestock, the role of this mycobacteria needs to be assessed since it might interfere with current M. bovis / M. caprae surveillance strategies. To better understand the pathogenesis of TB in voles, an experimental infection model was set up to reproduce M. microti infection in laboratory Bank voles (Myodes glareolus). Two infection routes (intragastric and intraperitoneal) and doses (10 5 and 10 6 CFU/0.1 mL) were assessed. Voles were culled at different post-infection time points. Serology, histopathology, acid-fast bacilli staining, qPCR, and mycobacterial culture from tissues were performed. In addition, qPCR from feces and oral swabs were conducted to assess bacterial shedding. The model allowed us to faithfully reproduce the disease phenotype described in free-ranging voles and characterize the pathogenesis of the infection. Most animals showed multifocal and diffuse granulomatous lesions in the liver and spleen, respectively. Less frequently, granulomas were observed in lungs, lymph nodes, muscles, and salivary gland. Mycobacterial DNA was detected in feces from a few animals but not in oral swabs. However, one contact uninfected vole seroconverted and showed incipient TB compatible lesions, suggesting horizontal transmission between voles.

Published
2022

23. Additional file 2 of A Bayesian evolutionary model towards understanding wildlife contribution to F4-family Mycobacterium bovis transmission in the South-West of France

Author

Duault, Hélène, Michelet, Lorraine, Boschiroli, Maria-Laura, Durand, Benoit, and Canini, Laetitia

Abstract

Additional file 2: Parameters estimation (median and 95% High Posterior Density (HPD) interval) and effective sample size (ESS). Ne is the effective population size and ESS stands for effective sample size.

Published
2022
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24. Additional file 1 of A Bayesian evolutionary model towards understanding wildlife contribution to F4-family Mycobacterium bovis transmission in the South-West of France

Author

Duault, Hélène, Michelet, Lorraine, Boschiroli, Maria-Laura, Durand, Benoit, and Canini, Laetitia

Abstract

Additional file 1: Model selection with the Bayes Factor. N corresponds to the number of particles, ML1 (2) to the log maximum likelihood of model 1 (2) and SD to the standard deviation. Log(BF) is the difference between ML1 and ML2. If (BF) > 0 (< 0) than model 1 (2) is favored. “-” means that the results were inconclusive. Subpopulations defined by host-species are not taken into account.

Published
2022
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25. Additional file 5 of A Bayesian evolutionary model towards understanding wildlife contribution to F4-family Mycobacterium bovis transmission in the South-West of France

Author

Duault, Hélène, Michelet, Lorraine, Boschiroli, Maria-Laura, Durand, Benoit, and Canini, Laetitia

Abstract

Additional file 5: Number of inter-species transitions per tree calculated over 1004 sampled trees. Number of transitions are represented according to transition type and various probability thresholds (0.7, 0.8 and 0.9).

Published
2022
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26. Experimental Infection of Captive Red Foxes (Vulpes vulpes) with Mycobacterium bovis

Author

Richomme, Céline, primary, Lesellier, Sandrine, additional, Salguero, Francisco Javier, additional, Barrat, Jacques Laurent, additional, Boucher, Jean-Marc, additional, Reyes-Reyes, Jennifer Danaidae, additional, Hénault, Sylvie, additional, De Cruz, Krystel De, additional, Tambosco, Jennifer, additional, Michelet, Lorraine, additional, Boutet, Justine, additional, Elahi, Rubyat, additional, Lyashchenko, Konstantin P., additional, O’Halloran, Conor, additional, Balseiro, Ana, additional, and Boschiroli, Maria Laura, additional

Published
2022
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27. Experimental Mycobacterium microti Infection in Bank Voles (Myodes glareolus)

Author

Vidal, Enric, primary, Burgaya, Judit, additional, Michelet, Lorraine, additional, Arrieta-Villegas, Claudia, additional, Cantero, Guillermo, additional, de Cruz, Krystel, additional, Tambosco, Jennifer, additional, Di Bari, Michelle, additional, Romolo, Nonno, additional, Boschiroli, Maria Laura, additional, and Pérez de Val, Bernat, additional

Published
2022
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38. IS 6110 Copy Number in Multi-Host Mycobacterium bovis Strains Circulating in Bovine Tuberculosis Endemic French Regions.

Author

Charles, Ciriac, Conde, Cyril, Biet, Franck, Boschiroli, Maria Laura, and Michelet, Lorraine

Subjects
TUBERCULOSIS in cattle, TUBERCULOSIS, MYCOBACTERIUM tuberculosis, MYCOBACTERIUM bovis, BACTERIAL evolution, BACTERIAL genomes, GENETIC variation
Abstract

IS 6110 is an insertion sequence found in the Mycobacterium tuberculosis complex, to which Mycobacterium bovis belongs, which can play a role in genome plasticity and in bacterial evolution. In this study, the abundance and location of IS 6110 on M. bovis genomic data of French animal field strains were studied. A first analysis was performed on a panel of 81 strains that reflect the national M. bovis population's genetic diversity. The results show that more than one-third of them are IS 6110 multicopy and that 10% have IS 6110 in a high copy number (more than 6 copies). Multicopy strains are those circulating in the regions where prevalence was above the national average. Further study of 93 such strains, with an IS 6110 copy number of 10-12, showed stability of IS 6110 copy number and genome location over time and between host species. The correlation between M. bovis multicopy strains and high bovine tuberculosis (bTB) prevalence leads us to consider whether their epidemiological success could be partly due to genetic changes originated by IS 6110 transposition. [ABSTRACT FROM AUTHOR]

Published
2022
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39. MOESM1 of Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum

Author

Lesellier, Sandrine, Maria-Laura Boschiroli, Barrat, Jacques, Wanke, Christoph, Salguero, Francisco, Garcia-Jimenez, Waldo, Nunez, Alex, Godinho, Ana, Spiropoulos, John, Palmer, Simonette, Dipesh Dave, Anderson, Paul, Jean-Marc Boucher, Krystel De Cruz, Henault, Sylvie, Michelet, Lorraine, Gowtage, Sonya, Williams, Gareth, Nadian, Allan, Monchâtre-Leroy, Elodie, Boué, Frank, Chambers, Mark, and Richomme, Céline

Subjects
bacterial infections and mycoses
Abstract

Additional file 1: Table S1. Primers used for the detection of mycobacteria by RT-PCR

Published
2019
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40. Accurate phylogenetic relationships among Mycobacterium bovis strains circulating in France based on whole genome sequencing and single nucleotide polymorphism analysis

Author

Hauer, Amandine, Michelet, Lorraine, Cochard, Thierry, Branger, Maxime, Nunez, Javier, Boschiroli, Maria-Laura, and Biet, Franck

Subjects
génotypage, Microbiology and Parasitology, tuberculose bovine, séquençage du génome complet, Mycobacterium bovis, bovine tuberculosis, phylogenetic, SNP, WGS, evolution, analyse phylogénétique, Microbiologie et Parasitologie
Abstract

In recent years the diversity of the French Mycobacterium bovis population responsible for bovine tuberculosis (bTB) outbreaks since 1970 has been described in detail. To further understand bTB evolution in France, we used single nucleotide polymorphisms (SNPs) based on whole genome sequence versus classical genotyping methods in order to identify accurate phylogenetic relationships between M. bovis strains. Whole genome sequencing was carried out on a selection of 87 strains which reflect the French M. bovis population’s genetic diversity. Sequences were compared to the M. bovis reference genome AF2122/97. Comparison among the 87 genomes revealed 9,170 sites where at least one strain shows a SNP with respect to the reference genome; 1,172 are intergenic and 7,998 in coding sequences, of which 2,880 are synonymous and 5,118 non-synonymous. SNP-based phylogenetic analysis using these 9,170 SNP is congruent with the cluster defined by spoligotyping and multilocus variable number of tandem repeat analysis typing. In addition, some SNPs were identified as specific to genotypic groups. These findings suggest new SNP targets that can be used for the development of high-resolving methods for genotyping as well as for studying M. bovis evolution and transmission patterns. The detection of non-synonymous SNPs on virulence genes enabled us to distinguish different clusters. Our results seem to indicate that genetically differentiated clusters could also display distinctive phenotypic traits.

Published
2019

41. Séquençage et analyse du génome complet de la souche de Mycobacterium bovis emblématique des cas de tuberculose bovine contemporaine en France

Author

BRANGER, Maxime, Michelet, Lorraine, Karoui, Claudine, Cochard, Thierry, De Cruz, Krystel, Hénault, Sylvie, Biet, Franck, Boschiroli, María Laura, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, séquençage, Tuberculose, spoligotype, Mycobacterium bovis, ComputingMilieux_MISCELLANEOUS, génomique
Abstract

National audience

Published
2018

42. Tuberculose bovine en France : évolution des souches de Mycobacterium bovis étudiée par approche génomique

Author

Michelet, Lorraine, Hauer, Amandine, BRANGER, Maxime, Karoui, Claudine, Cochard, Thierry, De Cruz, Krystel, Hénault, Sylvie, Boschiroli, María Laura, Biet, Franck, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), and Institut National de la Recherche Agronomique (INRA)-Université de Tours

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Animaux de rente, Tuberculose, Zoonose, Epidémiologie génomique, ComputingMilieux_MISCELLANEOUS, Faune sauvage
Abstract

National audience

Published
2018

44. Detection of live M. bovis BCG in tissues and IFN-γ responses in European badgers (Meles meles) vaccinated by oropharyngeal instillation or directly in the ileum

Author

Lesellier, Sandrine, primary, Boschiroli, Maria-Laura, additional, Barrat, Jacques, additional, Wanke, Christoph, additional, Salguero, Francisco J., additional, Garcia-Jimenez, Waldo L., additional, Nunez, Alex, additional, Godinho, Ana, additional, Spiropoulos, John, additional, Palmer, Simonette, additional, Dave, Dipesh, additional, Anderson, Paul, additional, Boucher, Jean-Marc, additional, de Cruz, Krystel, additional, Henault, Sylvie, additional, Michelet, Lorraine, additional, Gowtage, Sonya, additional, Williams, Gareth A., additional, Nadian, Allan K., additional, Monchâtre-Leroy, Elodie, additional, Boué, Frank, additional, Chambers, Mark A., additional, and Richomme, Céline, additional

Published
2019
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47. Diversité génétique des souches françaises de M. bovis d’origine animale: contribution du WGS

Author

michelet, Lorraine, BRANGER, Maxime, Cochard, Thierry, De Cruz, Krystel, Karoui, Claudine, Hénault, Sylvie, Biet, Franck, Boschiroli, María Laura, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours, Société Française de Microbiologie (SFM). FRA., and Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2017

50. Prevalence of tick-borne viruses inIxodes ricinusassessed by high-throughput real-time PCR

Author

Gondard, Mathilde, primary, Michelet, Lorraine, additional, Nisavanh, Athinna, additional, Devillers, Elodie, additional, Delannoy, Sabine, additional, Fach, Patrick, additional, Aspan, Anna, additional, Ullman, Karin, additional, Chirico, Jan, additional, Hoffmann, Bernd, additional, van der Wal, Fimme Jan, additional, de Koeijer, Aline, additional, van Solt-Smits, Conny, additional, Jahfari, Seta, additional, Sprong, Hein, additional, Mansfield, Karen L, additional, Fooks, Anthony R, additional, Klitgaard, Kirstine, additional, Bødker, Rene, additional, and Moutailler, Sara, additional

Published
2018
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