Search

Your search keyword '"mice db/db"' showing total 2 results
Start Over Descriptor "mice db/db"
2 results on '"mice db/db"'

1. Lowered levels of nicotinic acetylcholine receptors and elevated apoptosis in the hippocampus of brains from patients with type 2 diabetes mellitus and db/db mice

Author

Yang-Ting Dong, Yi Xu, Zhi-Zhong Guan, Wen-Feng Yu, Kun Cao, Jie Xiang, Yan Xiao, Xiao-Lan Qi, and Bing Guo

Subjects
Male, Aging, medicine.medical_specialty, alpha7 Nicotinic Acetylcholine Receptor, type 2 diabetes mellitus, Spatial Learning, Hippocampus, Morris water navigation task, Apoptosis, Receptors, Nicotinic, Pathogenesis, Mice, mice db/db, Memory, Internal medicine, medicine, Animals, Humans, Cognitive Dysfunction, Maze Learning, Aged, postmortem human brain, Acetylcholine receptor, TUNEL assay, business.industry, Type 2 Diabetes Mellitus, Cell Biology, Endocrinology, Nicotinic agonist, Diabetes Mellitus, Type 2, nervous system, Female, nicotinic acetylcholine receptors, Autopsy, learning and memory, business, Research Paper
Abstract

Cognitive impairment caused by diabetes has been gradually recognized. Generally, nicotinic acetylcholine receptors (nAChRs) play an important role in the pathogenesis in dementia disorders including Alzheimer's disease (AD). However, the expression of nAChRs in the brains of type 2 diabetes mellitus (T2DM) is unexplored. This study explored the alterations of nAChRs in the postmortem brains of patients with T2DM and brains of db/db mice. Morris water maze test was used to appraise the ability of spatial learning and memory; Western blotting and RT-qPCR were performed to determine the expressions of target protein and mRNA, respectively; TUNEL was used to detect the apoptosis of neurons. We found that the protein levels of nAChR α7 and α4 subunits were significantly decreased and the apoptosis rates in neurons elevated in the hippocampus of T2DM patients and db/db mice as comparison to controls. Furthermore, the db/db mice exhibited the impaired cognition, the elevated level of pro-apoptotic protein and the reduced level of anti-apoptotic and synaptic proteins. This study shows the lowered level of nAChR α7 and α4 subunits and the elevated apoptosis in the hippocampus of T2DM patients and db/db mice, which might help explain the impaired cognition in T2DM.

Published
2020

2. Lowered levels of nicotinic acetylcholine receptors and elevated apoptosis in the hippocampus of brains from patients with type 2 diabetes mellitus and db/db mice.

Author

Xu Y, Cao K, Guo B, Xiang J, Dong YT, Qi XL, Yu WF, Xiao Y, and Guan ZZ

Subjects
Aged, Animals, Autopsy, Cognitive Dysfunction, Female, Humans, Male, Maze Learning, Memory, Mice, Spatial Learning, alpha7 Nicotinic Acetylcholine Receptor metabolism, Apoptosis, Diabetes Mellitus, Type 2 metabolism, Diabetes Mellitus, Type 2 pathology, Hippocampus pathology, Receptors, Nicotinic metabolism
Abstract

Cognitive impairment caused by diabetes has been gradually recognized. Generally, nicotinic acetylcholine receptors (nAChRs) play an important role in the pathogenesis in dementia disorders including Alzheimer's disease (AD). However, the expression of nAChRs in the brains of type 2 diabetes mellitus (T2DM) is unexplored. This study explored the alterations of nAChRs in the postmortem brains of patients with T2DM and brains of db/db mice. Morris water maze test was used to appraise the ability of spatial learning and memory; Western blotting and RT-qPCR were performed to determine the expressions of target protein and mRNA, respectively; TUNEL was used to detect the apoptosis of neurons. We found that the protein levels of nAChR α7 and α4 subunits were significantly decreased and the apoptosis rates in neurons elevated in the hippocampus of T2DM patients and db/db mice as comparison to controls. Furthermore, the db/db mice exhibited the impaired cognition, the elevated level of pro-apoptotic protein and the reduced level of anti-apoptotic and synaptic proteins. This study shows the lowered level of nAChR α7 and α4 subunits and the elevated apoptosis in the hippocampus of T2DM patients and db/db mice, which might help explain the impaired cognition in T2DM.

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results