1. miR-6745-TIMP1 axis inhibits cell growth and metastasis in gastric cancer
- Author
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Tong-Cun Zhang, Hui Liu, Shi-Qiang Fang, Zhi-Wen Wang, Xing-Hua Liao, Yuan Xiang, Xiao-Yu Zhang, and Qi-Bei Zong
- Subjects
Male ,TIMP1 ,Aging ,miR-6745 ,Mice, Nude ,medicine.disease_cause ,Metastasis ,Mice ,Stomach Neoplasms ,oncogenesis ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Wnt/β-catenin pathway ,Gene silencing ,Neoplasm Metastasis ,Wnt Signaling Pathway ,Cell Proliferation ,Tissue Inhibitor of Metalloproteinase-1 ,Oncogene ,Chemistry ,Cell growth ,gastric cancer ,Stomach ,Wnt signaling pathway ,Cancer ,Cell Biology ,medicine.disease ,MicroRNAs ,Cancer research ,Carcinogenesis ,Research Paper - Abstract
Tissue inhibitor matrix metalloproteinase 1 (TIMP1) has been reported to act as a tumor oncogene in colon cancer. However, little is known about the biological role of TIMP1 in gastric cancer. In this study, we found that the expression of TIMP1 in GC tissues was upregulated compared with the normal gastric tissues. TIMP1 was confirmed as a direct target of miR-6745 and silencing TIMP1 mimicked the effects of miR-6745 in GC cells. Further mechanism studies have shown that miR-6745 inhibits the Wnt/β-catenin pathway by targeting TIMP1, thereby inhibiting cell proliferation, migration and invasion. In addition, through the analysis of GC tissues, a negative correlation between miR-6745 and TIMP1 was found in 42 GC tissues. Our findings indicate that the miR-6745-TIMP1 axis regulates Wnt/βcatenin signaling and participates in GC tumorigenesis and provide a potential therapeutic target for preventing GC progression.
- Published
- 2021
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