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The purpose of this study was to determine the impact of feeding time on NPY/AgRP protein and mRNA expression in the brain during prenatal and postnatal periods. Feeding at different times of the day during pregnancy may have lasting effects on the hypothalamic circuitry in offspring energy homeostasis. In the experimental design, adult female hamsters were randomly assigned to receive three different feeding conditions: ad libitum, night-time feeding and day-time feeding groups. After the lactation period, feeding regimens were continued for the offspring born from mothers who were given nutritional regimens, until they were 30 days old. RT-PCR for NPY/AgRP mRNA expression and ELISA analysis for protein levels were performed on hypothalamus tissues of hamsters at 10, 20 and 30 days of postnatal stage. There was no difference between the groups in terms of the daily measured food consumption of the offspring's. Body weights were significantly decreased in both night-time and day-time feeding groups compared to the ad libitum group (p<0.05). The highest increase in mRNA expression of NPY/AgRP was seen in the samples taken at 10. day of lactation in the night-time and day-time feeding groups of the offspring treated with three different feeding regimens. When the feeding regimes were compared, it was observed that the protein and mRNA expression of both NPY and AGRP increased the most in the offspring groups in which the feeding was restricted night -time only. Early stages of development have shown that maternal factors have significantly affected the offspring NPY and AgRP mRNA expression and protein levels. These results show that the metabolic regulation of energy balance may change with maternal factors during the very early stages of development. [ABSTRACT FROM AUTHOR]

Benzotriazole ultraviolet stabilizers (BUVSs) are a group of widely used chemicals added to a variety of consumer (e.g., plastics) and industrial (e.g., metal coating) goods. Although detected globally as an environmentally persistent pollutant, BUVSs have received relatively little toxicological attention and only recently have been acknowledged to affect development and the endocrine system in vivo. In our previous study, altered behavior, indicative of potential neurotoxicity, was observed among rainbow trout alevins (day 14 posthatching) that were microinjected as embryos with a single environmentally relevant dose of 2,4‐di‐tert‐butyl–6‐(5‐chloro‐2H‐benzotriazol‐2‐yl) phenol (UV‐327). In the present follow‐up study, we performed whole‐transcriptome profiling (RNA sequencing) of newly hatched alevins from the same batch. The primary aim was to identify biomarkers related to behavior and neurology. Dose‐specifically, 1 to 176 differentially expressed genes (DEGs) were identified. In the group presenting altered behavior (273.4 ng g−1), 176 DEGs were identified, yet only a fraction was related to neurological functions, including water, calcium, and potassium homeostasis; acetylcholine transmission and signaling; as well insulin and energy metabolism. The second objective was to estimate the transcriptomic point of departure (tPOD) and assess if point estimate(s) are protective of altered behavior. A tPOD was established at 35 to 94 ng UV‐327 g−1 egg, making this tPOD protective of behavioral alterations. Holistically, these transcriptomic alterations provide a foundation for future research on how BUVSs can influence rainbow trout alevin development, while providing support to the hypothesis that UV‐327 can influence neurogenesis and subsequent behavioral endpoints. The exact structural and functional changes caused by embryonic exposure to UV‐327 remain enigmatic and will require extensive investigation before being deciphered and understood toxicologically. Environ Toxicol Chem 2024;43:2026–2038. © 2024 The Author(s). Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. [ABSTRACT FROM AUTHOR]

Persistent organic pollutants pose a great threat to amphibian populations, but information on the bioaccumulation of contaminants in amphibians remains scarce. To examine the tissue distribution and maternal transfer of organic halogenated pollutants (OHPs) in frogs, seven types of tissues from black‐spotted frog (muscle, liver, kidney, stomach, intestine, heart, and egg) were collected from an e‐waste–polluted area in South China. Among the seven frog tissues, median total OHP concentrations of 2.3 to 9.7 μg/g lipid weight were found (in 31 polychlorinated biphenyl [PCB] individuals and 15 polybrominated diphenyl ether [PBDE], dechlorane plus [syn‐DP and anti‐DP], bexabromobenzene [HBB], polybrominated biphenyl] PBB153 and ‐209], and decabromodiphenyl ethane [DBDPE] individuals). Sex‐specific differences in contaminant concentration and compound compositions were observed among the frog tissues, and eggs had a significantly higher contaminant burden on the whole body of female frogs. In addition, a significant sex difference in the concentration ratios of other tissues to the liver was observed in most tissues except for muscle. These results suggest that egg production may involve the mobilization of other maternal tissues besides muscle, which resulted in the sex‐specific distribution. Different parental tissues had similar maternal transfer mechanisms; factors other than lipophilicity (e.g., molecular size and proteinophilic characteristics) could influence the maternal transfer of OHPs in frogs. Environ Toxicol Chem 2024;43:1557–1568. © 2024 SETAC [ABSTRACT FROM AUTHOR]

In this study, concentrations of Fe, Zn, As, Cd, Hg, Pb, and Cu in blood and muscle samples and maternal transfer of these elements to eggs and hatchlings were investigated for hawksbill turtles on Ommolgorm, Nakhiloo, and Kharko Islands from the Persian Gulf. The results indicate a significant difference (p≤0.05) between the accumulations of the elements between the rookeries. On three islands, the highest and lowest concentrations of Fe were observed in the yolk and albumin, respectively. The highest concentrations of Zn were found in yolk and maternal muscle from Ommolgorm and Nakhiloo islands, and the lowest concentration of Zn was found in hatchling and abnormal albumin at Kharko Island. The highest amount of As was present in maternal blood, and the lowest was in an abnormal egg on three islands. The highest amount of Hg was in maternal blood and muscle, and the lowest was in normal and abnormal eggs. The highest Pb concentration was in yolk and the lowest was in maternal muscle and blood in Ommolgorm and Nakhiloo Island; however, at Kharko Island, the highest and lowest concentrations were in yolk and normal albumin, respectively. Cd and Cu were ND in the most tissues. Fe, As, Hg, and Pb were higher than Cd and Cu in the maternal blood. The accumulation of heavy metals in the yolk, blood, and hatchling was higher than in other tissues. The accumulation of some elements in samples from Kharko Island was higher than on the other two islands. Based on the results, a significant relationship was found among the concentrations of elements in the maternal blood, yolk, and hatchling tissue. Our results showed a positive correlation between the concentrations in the maternal blood and yolk and hatchling tissue, which confirms maternal transfer of the elements. [ABSTRACT FROM AUTHOR]

Clopidol is extensively used in livestock farming and residues of this antibiotic can persist in animal tissues, posing a risk to humans and the environment. In this study, we investigated the depletion of clopidol in various edible tissues of chickens (muscle, liver, kidney, fat, and eggs) using liquid chromatography–tandem mass spectrometry after the administration of a clopidol-contaminated diet (at 250 mg kg−1 for the high (1x) dose). After 14 d of exposure, the clopidol concentrations were highest in eggs (median: 9.83 mg/kg), followed by liver (3.56 mg/kg), kidney (3.01 mg/kg), muscle (1.56 mg/kg), and fat (0.727 mg/kg) at low exposure group, indicating that clopidol accumulated primarily in eggs rather than the other edible tissues. In addition, the maternal transfer ratios were estimated, and the transfer efficiencies of clopidol in muscle (egg-to-tissue ratio, ETR:1.81) and fat (2.06–58.2) were higher than those in liver (0.731–31.1) and kidney (0.832–38.9). Furthermore, we conducted a cumulative risk assessment for clopidol in edible chicken tissues using the hazard quotient (HQ) method. This assessment revealed that the exposure levels for Korean consumers pose an acceptable risk. However, for eggs from the 1x dose exposure group, the HQ values were greater than 1 for all age groups, particularly for young children (<18 y), suggesting that the higher daily consumption of eggs combined with the higher clopidol residues in eggs resulted in higher HQ values, which requires further attention. The findings of this study can assist in the management and monitoring of clopidol residues in chicken tissues and eggs [ABSTRACT FROM AUTHOR]

Maternal signals shape embryonic development, and in turn post-natal phenotypes. RNA deposition is one such method of maternal signalling and circadian rhythms are one trait thought to be maternally inherited, through this mechanism. These maternal circadian gene transcripts aid development of a functioning circadian system. There is increasing evidence that maternal signals can be modified, depending on prevailing environmental conditions to optimise offspring fitness. However, currently, it is unknown if maternal circadian gene transcripts, and consequently early embryonic gene transcription, are altered by maternal developmental conditions. Here, using avian mothers who experienced either pre-natal corticosterone exposure, and/or post-natal stress as juveniles we were able to determine the effects of the timing of stress on downstream circadian RNA deposition in offspring. We demonstrated that maternal developmental history does indeed affect transfer of offspring circadian genes, but the timing of stress was important. Avian mothers who experienced stress during the first 2 weeks of post-natal life increased maternally deposited transcript levels of two core circadian clock genes, BMAL1 and PER2. These differences in transcript levels were transient and disappeared at the point of embryonic genome transcription. Pre-natal maternal stress alone was found to elicit delayed changes in circadian gene expression. After activation of the embryonic genome, both BMAL1 and PER2 expression were significantly decreased. If both pre-natal and post-natal stress occurred, then initial maternal transcript levels of BMAL1 were significantly increased. Taken together, these results suggest that developmental stress differentially produces persistent transgenerational effects on offspring circadian genes. [ABSTRACT FROM AUTHOR]

Benzotriazole ultraviolet (UV) stabilizers (BUVSs) are used in great quantities during industrial production of a variety of consumer and industrial goods. As a result of leaching and spill, BUVSs are detectable ubiquitously in the environment. As of May 2023, citing concerns related to bioaccumulation, biomagnification, and environmental persistence, (B)UV(S)‐328 was recommended to be listed under Annex A of the Stockholm Convention on Persistent Organic Pollutants. However, a phaseout of UV‐328 could result in a regrettable substitution because the replacement chemical(s) could cause similar or unpredicted toxicity in vivo, relative to UV‐328. Therefore, the influence of UV‐327, a potential replacement of UV‐328, was investigated with respect to early life development of newly fertilized rainbow trout embryos (Oncorhynchus mykiss), microinjected with environmentally relevant concentrations of UV‐327. Developmental parameters (standard length), energy consumption (yolk area), heart function, blue sac disease, mortality, and behavior were investigated. Alevins at 14 days posthatching, exposed to 107 ng UV‐327 g−1 egg, presented significant signs of hyperactivity; they moved on average 1.8‐fold the distance and at 1.5‐fold the velocity of controls. Although a substantial reduction in body burden of UV‐327 was observed at hatching, it is postulated that UV‐327, due to its lipophilic properties, interfered with neurological development and signaling from the onset of neurogenesis. If these results hold true across multiple taxa and species, a potential contributor to neurodevelopmental disorders might have been identified. These findings suggest that UV‐327 poses an unknown hazard to rainbow trout embryos and alevins, rendering UV‐327 a potential regrettable substitution to UV‐328. However, a qualified statement on a regrettable substitution requires a comparative investigation on the teratogenic effects between the two BUVSs. Environ Toxicol Chem 2024;43:762–771. © 2023 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. [ABSTRACT FROM AUTHOR]

Since 2013, loggerhead sea turtle (Caretta caretta, Linnaeus 1758) nesting has been observed further north along the Italian coast, reaching the Tuscan coast (NW Mediterranean Sea). The four nesting events that occurred in Tuscany in the summer of 2019 spurred the scientific community to monitor these occurrences more carefully, following them from egg deposition to hatching. This provided an opportunity to collect samples for conducting multidisciplinary investigations, including the toxicological investigations of the biological material collected from the four nests. The aim of this study was to conduct an initial assessment of persistent organic pollutants in the eggs laid in Tuscany, aiming to establish a baseline on this topic for subsequent nesting events that have occurred until today. Organochlorine compounds (OCs), specifically polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethane (DDT) and its metabolites, and hexachlorobenzene (HCB), were analyzed and detected in unhatched eggs, embryos, and chorio-allantoic membranes (CAMs). OCs were detected in all samples, with PCBs > DDTs » HCB. A significant spatial variation in pollutant levels and profiles among sea turtle nesting locations was found. Embryos showed higher levels of contamination than egg contents regardless of the developmental stages. Depth of the laying chamber and egg mass were not significant factors in OC bioaccumulation. For the first time in the Mediterranean Sea, this study assessed the role of CAM in the transfer of contaminants to the embryo. Overall, the OC levels found were lower compared to the results from other studies conducted worldwide on loggerhead sea turtle biological material. This was the first assessment of nest biological material for the North-Western coast of Italy. [ABSTRACT FROM AUTHOR]

Since 2013, loggerhead sea turtle (Caretta caretta, Linnaeus 1758) nesting has been observed further north along the Italian coast, reaching the Tuscan coast (NW Mediterranean Sea). The four nesting events that occurred in Tuscany in the summer of 2019 spurred the scientific community to monitor these occurrences more carefully, following them from egg deposition to hatching. This provided an opportunity to collect samples for conducting multidisciplinary investigations, including the toxicological investigations of the biological material collected from the four nests. The aim of this study was to conduct an initial assessment of persistent organic pollutants in the eggs laid in Tuscany, aiming to establish a baseline on this topic for subsequent nesting events that have occurred until today. Organochlorine compounds (OCs), specifically polychlorinated biphenyls (PCBs), dichlorodiphenyltrichloroethane (DDT) and its metabolites, and hexachlorobenzene (HCB), were analyzed and detected in unhatched eggs, embryos, and chorio-allantoic membranes (CAMs). OCs were detected in all samples, with PCBs > DDTs ≫ HCB. A significant spatial variation in pollutant levels and profiles among sea turtle nesting locations was found. Embryos showed higher levels of contamination than egg contents regardless of the developmental stages. Depth of the laying chamber and egg mass were not significant factors in OC bioaccumulation. For the first time in the Mediterranean Sea, this study assessed the role of CAM in the transfer of contaminants to the embryo. Overall, the OC levels found were lower compared to the results from other studies conducted worldwide on loggerhead sea turtle biological material. This was the first assessment of nest biological material for the North-Western coast of Italy.

Mercury (Hg) is known to be maternally transferred during embryonic development in sharks; however, Hg concentrations in embryos of filter feeding shark species have not previously been reported. This study measured the total Hg (THg) concentration in muscle tissue of 27 embryos taken from a pregnant whale shark (Rhincodon typus) landed in Taiwan in 1995 and the mean THg concentration compared to the mean muscle THg concentration in embryos from other shark species. The mean (± standard deviation) THg concentration in whale shark embryos was 0.0762 ± 0.0163 µg/g dry weight (0.0224 ± 0.0054 µg/g wet weight). There was no relationship between muscle THg concentration and body length and no significant difference in THg concentration between male and female embryos (p > 0.05). Whale shark embryos have the lowest reported muscle THg concentrations compared to literature values for muscle THg concentrations for embryos from other shark species. [ABSTRACT FROM AUTHOR]

The contamination of uranium in aquatic ecosystems has raised growing global concern. However, the understanding of its chronic effects on aquatic organisms is limited, particularly with regards to transgenerational toxicity. In this study, we evaluated the maternal transfer risk of uranium using zebrafish. Sexually mature female zebrafish were exposed to 2 and 20 ng/g of uranium-spiked food for 28 days. The induced bioconcentration, thyroid disruption, and oxidative stress in both the adults (F0) and their embryos (F1) were further investigated. Element analysis showed that uranium was present in both F0 and F1, with higher concentrations observed in F1, indicating significant maternal offloading to the offspring. Meanwhile, an increased malformation and decreased swim speed were observed in the F1. Thyroid hormone analysis revealed significant decreases in the levels of triiodothyronine (T3) in both the F0 adults and F1 embryos, but thyroxine (T4) was not significantly affected. Additionally, the activities of antioxidant defenses, including catalase (CAT) and superoxide dismutase (SOD), and the expression of glutathione (GSH) and malondialdehyde (MDA) were significantly altered in the F0 and F1 larvae at 120 hpf. The hypothalamic-pituitary-thyroid (HPT) axis, oxidative stress, and apoptosis-related gene transcription expression were also significantly affected in both generations. Taken together, these findings highlight the importance of considering maternal transfer in uranium risk assessments.

Hexabromocyclododecane (HBCD) is a persistent organic pollutant that has been characterized as an endocrine disruptor, undergoes maternal transfer, and hinders development and growth in oviparous organisms. The present study examined the apical effects of dietary HBCD (11.5, 36.4, 106 mg/kg, wet wt) on adult fathead minnow exposed for 49 days and the subsequent accumulation and maternal transfer kinetics in adult tissue and eggs, respectively. Exposed adults displayed a significant increase in egg production in the medium treatment group, but no other significant effects were noted. Maternal transfer of dietary HBCD had a similar egg‐to‐muscle ratios (EMR) in the low and medium treatment groups (1.65 and 1.27 [wet wt], respectively). However, the high treatment group deviated from other treatments with an EMR of 4.2 (wet wt), potentially due to differences in total lipid content in food and/or reaching diffusion/lipid saturation limits in adult tissue, resulting in lower accumulation in the adult muscle tissue. A positive correlation was observed between egg HBCD concentration and time of exposure, which indicates that maternal transfer of HBCD is of concern in fish, and further studies should be conducted to fully elucidate the potential adverse effects that may be observed in the early life stage of oviparous organisms. Environ Toxicol Chem 2023;42:143–153. © 2022 SETAC [ABSTRACT FROM AUTHOR]

Environmental contaminants with chemical origins, such as organochlorine pesticides (OCPs) have major impacts on the health of marine animals, including sea turtles, due to the bioaccumulation of those substances by transference throughout the food chain. The effects of environmental pollution on the health of marine turtles are very important for management strategies and conservation. During recent decades, the south Gulf of Mexico and the Yucatan Peninsula have suffered from increasingly frequent disturbances from continental landmasses, river systems, urban wastewater runoff, port areas, tourism, industrial activities, pesticides from agricultural use, and other pollutants, such as metals, persistent organic pollutants (POPs) and hydrocarbons (from the oil industry activities), which contaminate water and sediments and worsen the environmental quality of the marine ecosystem in this region. In this study, we assessed the concentrations of OCPs in the blood and eggs of 60 hawksbill turtles (Eretmochelys imbricata) nesting at the Punta Xen turtle camp, and their effects on the nesting population's reproductive performance: specifically, maternal transfer and embryonic development were analyzed. Hematologic characteristics, including packed cell volume, white blood cell count, red blood cell count, and haemoglobin levels, and plasma chemistry values, including creatinine, blood urea nitrogen, uric acid, triglyceride, total cholesterol and glucose, were also measured. The general health of the turtles in this study, as well as their levels of urea, serum creatinine, glucose, uric, acid, cholesterol, and triglyceride, fell within normal ranges and was similar to other normal values, which could indicate the turtles' good energy levels and body conditions for nest-building activity, with all of the turtles able to successfully come ashore to nest. All the same, the obtained results also indicate that OCPs affect the nesting and reproductive performance of the hawksbill turtles, as well as their fertility and the development of the population of eggs and reproductive performance, specifically in terms of maternal transference and embryonic development. There were significant differences in the concentrations of OCPs (ΣHCHs and ΣDienes) between maternal blood and eggs, indicating that these chemicals are transferred from nesting females to eggs and, ultimately, to hatchlings. OCPs may, therefore, have an effect on the health and reproductive performance of hawksbill turtles, both in terms of their fertility and egg development. Conservation strategies need to be species-specific, due to differences in feeding, and address the reasons for any decline, focusing on regional assessments. Thus, accurate and comparable monitoring data are necessary, which requires the standardization of monitoring protocols. [ABSTRACT FROM AUTHOR]

Contaminant monitoring programs use wild bird eggs, but determining whether measured concentrations elicit adverse effects relies on extrapolation from toxicity studies with avian model species. Here, we directly evaluated the relationships between whole embryo contaminant concentrations and mRNA expression in liver tissue of the double-crested cormorant ( Nannopterum auritum ). Eggs collected from three North American sites (one from Lake Erie and two from the Salish Sea) were artificially incubated until pipping. Hepatic mRNA was analyzed with an EcoToxChip quantitative polymerase chain reaction (qPCR) array containing 354 target genes. The remaining embryo was analyzed for mercury and 95 persistent organic contaminants. Lake Erie embryos had higher concentrations of most organic contaminants than those from the Salish Sea. Sparse partial least-squares regression analysis of contaminant and gene expression data indicated that chlorinated hydrocarbons, especially polychlorinated biphenyls (PCBs), were associated with variation in gene expression. Linear correlations revealed consistent pairwise associations between chlorinated contaminants and the expression of nine genes (seven genes with PCBs and two with β-hexachlorocyclohexane). Partial least-squares discriminant analysis identified embryos from the Lake Erie site accurately. Overall, gene expression in embryos from wild-collected eggs was associated with tissue contaminant concentrations, and thus, transcriptomic measurements may serve to identify individuals or populations affected biologically by contaminants.

To enable risk characterization of perfluorooctane sulfonic acid (PFOS) in extended chronic and multi-generational exposures, we assessed PFOS bioconcentration in zebrafish (Danio rerio) exposed continuously to environmentally-relevant PFOS concentrations (0, 0.1, 0.6, 3.2, 20, and 100 µg/L PFOS) through 180 days postfertilization (dpf) in parental (P) and first filial generation (F1) fish. Exposures included five replicate tanks per treatment where whole-body PFOS concentrations were measured using 20-35 fish per replicate at 14 and 29 dpf in the P generation and one fish of each sex per replicate at 180 dpf for the P and F1 generations. Perfluorooctane sulfonic acid accumulation reached an apparent steady state at ≤ 14 dpf where whole-body wet-weight concentrations remained constant through 180 dpf in the P and F1 generations. The median bioconcentration factor (BCF) of 934 L/kg was observed for all PFOS exposures with a range from 255 to 2,136 L/kg which varied with PFOS exposure concentration and sex of adult fish. Significantly lower BCFs were observed in 20 and 100 µg/L PFOS exposures versus 0.1 and 0.6 µg/L indicating exposure-concentration dependance. Additionally, males had significantly increased (∼2×) PFOS accumulation and BCFs versus females in both P and F1 generations. Maternal transfer of PFOS was observed from P females to F1 eggs where maternal whole-body and egg PFOS burdens were equivalent, suggesting PFOS transfer to eggs was not a depuration pathway. Finally, a toxicokinetic model was developed that reliably reproduced PFOS whole-body burdens (data within 1.64-fold of predicted values) across all exposure durations spanning the P and F1 generations, providing a tool for PFOS bioaccumulation predictions relevant for risk assessment of acute, chronic, and multi-generational exposures., (© The Author(s) 2025. Published by Oxford University Press on behalf of the Society of Environmental Toxicology and Chemistry.)

Pesticides could induce long-term impacts on aquatic ecosystem via transgenerational toxicity. However, for many chiral pesticides, the potential enantioselectivity of transgenerational toxicity has yet to be fully understood. In this study, we used zebrafish as models to evaluate the maternal transfer risk of tebuconazole (TEB), which is a chiral triazole fungicide currently used worldwide and has been frequently detected in surface waters. After 28-day food exposure (20 and 400 ng/g) to the two enantiomers of TEB (S- and R-TEB) in adult female zebrafish (F0), increased malformation rate and decreased swimming speed were found in F1 larvae, with R-TEB showing higher impacts than S-enantiomer. Additionally, enantioselective effects on the secretion of thyroid hormones (THs) and expression of TH-related key genes along the hypothalamic-pituitary-thyroid (HPT) axis were found in both F0 and F1 after maternal exposure. Both the two enantiomers significantly disrupted the triiodothyronine (T3) and thyroxine (T4) contents in F0 with different degrees, whereas in F1, significant effects were only found in R-TEB groups with decreasing of both T3 and T4 contents. Most of the HPT axis related genes in F0 were upregulated by TEB and more sensitive to R-TEB than to S-TEB. In contrast, most of the genes in F1 were downregulated by both R- and S-TEB, especially the genes that are primarily responsible for thyroid development and growth (Nkx2–1), TH synthesis (NIS and TSHꞵ) and metabolism (Deio1). Findings from this study highlight the key role of enantioselectivity in the ecological risk assessment of chiral pesticides through maternal transfer.

The maternal transfer of 15 elements was examined in Peruvian fur seal (Arctocephalus australis) dam and pup paired vibrissae (whiskers), serum, and milk samples collected from 2009 to 2019. Pup vibrissae, grown in utero, represented gestational transfer, while milk represented lactational transfer. Element concentrations, except arsenic, were highest in vibrissae compared to serum and milk for both dams and pups. Mean arsenic concentrations in pup vibrissae (0.44 μg/g) and milk (0.41 μg/g) were twice as high as dam vibrissae concentrations (0.19 μg/g) and nearly ten times higher than dam (0.06 μg/g) and pup serum (0.04 μg/g) concentrations. Mean arsenic concentrations from 2011 to 2019 increased in dam vibrissae (0.026 μg/g to 0.262 μg/g) and milk (0.361 μg/g to 0.484 μg/g). Pup vibrissae had significantly higher concentrations for 11 of the 15 elements analyzed compared to dam vibrissae, suggesting that element transfer is occurring through recent exposure and remobilization of elements from dam body stores. Potentially high concentrations of aluminum, arsenic, copper, and lead in pup tissues may impact their survival and population health. The impact of regional mining activities can contribute to elevated trace elements through runoff and pose a possible threat to local marine environments. [ABSTRACT FROM AUTHOR]

A pregnant hamster's exposure to changes in environmental factors, such as light, temperature and nutrition, may influence behavioural and physiological changes in offspring. In this study, dietary methyl donor supplementation was employed to examine the role of maternal diet on appetite, body weight, serum leptin levels and locomotor activity in male Syrian hamster offspring. Dams were fed a standard control (SC) or methyl donor‐supplemented (MDSD) diet through pregnancy and lactation. At birth, offspring were cross‐fostered to dams fed an SC or MDSD diet (SC‐MDSD and MDSD‐SC) or remained with their birth mothers (SC‐SC and MDSD‐MDSD). At weaning, offspring were fed a SC or MDSD diet until 60 days of age. Food intake, serum leptin levels and locomotor activity were measured from 30–60 days of age. Offspring fed a MDSD diet post‐weaning (MDSD‐MDSD and SC‐MDSD) consumed more than double the amount of food daily compared with offspring fed a SC diet post‐weaning (SC‐SC, MDSD‐SC). Interestingly, there were no observed differences in body weight among all four groups. Serum leptin levels at 60 days of age were depressed in offspring fed a MDSD diet post‐weaning (MDSD‐MDSD and SC‐MDSD). There were no observed differences in wheel running activity between the SC‐SC and MDSC‐SC groups. Wheel running activity was at least twice the amount in offspring fed a MDSD diet post‐weaning (SC‐MDSD and MDSD‐MDSD). Taken together, these results indicate that the timing of methyl donor supplementation appears to be an important factor during the development of offspring. [ABSTRACT FROM AUTHOR]

Abstract Background The breakdown of dead organic matter is driven by a diverse array of organisms and is an important process increasingly impacted by a range of contaminants. While many studies have documented how contaminants affect food webs that are fueled by decaying plant litters, much less is known about how contaminants affect organisms that rely on dead animal material. Here, we begin to explore the effects of food contamination—using silver nanoparticles (AgNPs) as a model contaminant—on the carrion beetle Nicrophorus vespilloides that buries carcasses of small vertebrates in soils as food source and larval nursing grounds. Results Our data show that a single ingestion of a non-lethal dose of 1 μg mL−1 AgNPs by adult female beetles does not affect overall gut microbial activity but results in shifts in the gut microbial community composition towards pathogens including Alcaligenes, Morganella, and Pseudomonas. While no effects were observed in offspring clutch size, some reductions were visible in clutch weight, number of larvae, and number of eclosing pupae in exposed N. vespilloides in comparison with controls. Repeated ingestion of AgNPs over several weeks led to a decrease in survival of adult beetles, suggesting that more environmentally realistic exposure scenarios can directly affect the success of carcass-feeding animals. Conclusions Sub-lethal carcass contamination with a model pollutant can affect the gut microbial composition in female beetles and reduce offspring fitness. This encourages consideration of currently overlooked propagation routes of contaminants through necrophagous food webs and inherent consequences for ecological and evolutionary processes.

Mercury (Hg) is a toxic contaminant of global concern and the impact on Arctic ecosystems, particularly in seabirds, is critical due to large-scale Hg transport towards polar regions and its biomagnification in marine trophic systems. While the adverse effects of Hg on reproductive processes in seabirds are established, the understanding of Hg maternal transfer pathways and their control on Hg reproductive toxicity is limited. The combination of Hg compounds speciation (inorganic mercury and monomethylmercury MMHg) and Hg stable isotope composition in the different egg compartments (yolk, albumen, membrane, and shell) before embryo development was investigated to provide information on (i) Hg maternal transfer mechanisms, (ii) influence of egg biochemical composition on Hg organotropism and (iii) proxies of inputs of Hg contamination. Eggs of three seabird species (the common eider, the black-legged kittiwake and the little auk) collected within the same breeding period (summer 2020) in East Greenland were investigated. For all seabirds, albumen and membrane, the most protein-rich compartments, were the most contaminated (from 1.2 to 2.7 μg g−1 for albumen and from 0.3 to 0.7 μg g−1 for membrane). In these two compartments, more than 82% of the total Hg amount was in the form of MMHg. Additionally, mass-dependent fractionation values (δ 202Hg) were higher in albumen and membrane in the three species. This result was mainly due the organotropism of MMHg as influenced by the biochemical properties and chemical binding affinity of these proteinous compartments. Among the different egg compartments, individuals and species, mass-independent fractionation values were comparable (mean ± sd were 0.99 ± 0.11‰, 0.78 ± 0.11‰, 0.03 ± 0.05‰, 0.04 ± 0.10‰ for Δ199Hg, Δ201Hg, Δ200Hg and Δ204Hg, respectively). We conclude that initial MMHg accumulated in the three species originated from Arctic environmental reservoirs exhibiting similar and low photodemethylation extent. This result suggests a unique major source of MMHg in those ecosystems, potentially influenced by sea ice cover. [Display omitted] • Protein-rich egg compartments exhibited highest Hg contamination, mostly as MMHg. • MDF values likely reflected Hg organotropism for protein-rich compartments. • Odd-isotopes MIF indicated a single origin of MMHg contamination among seabirds. • Egg membranes/shells are non-invasive indicators of the source of Hg contamination. [ABSTRACT FROM AUTHOR]

Mercury (Hg) is a naturally occurring heavy metal, but human activities and natural processes have led to increased pollution with Hg in the environment. Organic mercury, such as methyl mercury (MeHg), is considered more toxic than most inorganic mercury compounds. MeHg is rapidly taken up by aquatic organisms and bioaccumulates through the aquatic food web. The bioaccumulation causes high levels of MeHg in apex predators, such as pilot whales. Pilot whale meat is a traditional food source on the Faroe Islands; thus the consumption of pilot whale meat can lead to high Hg exposures in humans. The majority of the total Hg in pilot whale and fish is generally assumed to be MeHg. However, the relative amount of MeHg to total Hg can be highly variable. For risk assessment, it is relevant to know both the MeHg and the total Hg content. This study summarizes the knowledge of muscle MeHg concentrations relative to total Hg concentrations in pilot whales in the Faroe Islands. The pilot whale tissue was sampled during 1977–78, 1986–87, 2009–2010, and 2015. The 2015 samples included two pairs of fetuses/mothers. The results showed that the 1977–78 pilot whale muscle samples had lower relative concentrations of MeHg to total Hg compared to samples from the subsequent years. This discrepancy between early and later years could not solely be explained by increased demethylation related to concentration differences. Instead, the difference is more likely explained by variations in relative amounts of MeHg in prey of the pilot whales. In the fetuses the total Hg concentration was 20% of the Hg concentration in the mother. The relative MeHg concentrations in the fetuses were also lower (∼20%–30%) than in the mother. However, the MeHg to total Hg fraction in the fetus was similar or higher than in the mother. [Display omitted] • MeHg/TotHg ratios were analyzed in pilot whale muscle from Faroe Islands. • Higher relative ratios were observed in 1980's and 2009–2015 than in late 1970's. • Variation was likely related to different MeHg concentrations in prey. • MeHg concentrations were similar or higher in fetus compared to mother. [ABSTRACT FROM AUTHOR]

Environmental contaminants with chemical origins, such as organochlorine pesticides (OCPs) have major impacts on the health of marine animals, including sea turtles, due to the bioaccumulation of those substances by transference throughout the food chain. The effects of environmental pollution on the health of marine turtles are very important for management strategies and conservation. During recent decades, the south Gulf of Mexico and the Yucatan Peninsula have suffered from increasingly frequent disturbances from continental landmasses, river systems, urban wastewater runoff, port areas, tourism, industrial activities, pesticides from agricultural use, and other pollutants, such as metals, persistent organic pollutants (POPs) and hydrocarbons (from the oil industry activities), which contaminate water and sediments and worsen the environmental quality of the marine ecosystem in this region. In this study, we assessed the concentrations of OCPs in the blood and eggs of 60 hawksbill turtles (Eretmochelys imbricata) nesting at the Punta Xen turtle camp, and their effects on the nesting population’s reproductive performance: specifically, maternal transfer and embryonic development were analyzed. Hematologic characteristics, including packed cell volume, white blood cell count, red blood cell count, and haemoglobin levels, and plasma chemistry values, including creatinine, blood urea nitrogen, uric acid, triglyceride, total cholesterol and glucose, were also measured. The general health of the turtles in this study, as well as their levels of urea, serum creatinine, glucose, uric, acid, cholesterol, and triglyceride, fell within normal ranges and was similar to other normal values, which could indicate the turtles’ good energy levels and body conditions for nest-building activity, with all of the turtles able to successfully come ashore to nest. All the same, the obtained results also indicate that OCPs affect the nesting and reproductive performance of the hawksbill turtles, as well as their fertility and the development of the population of eggs and reproductive performance, specifically in terms of maternal transference and embryonic development. There were significant differences in the concentrations of OCPs (ΣHCHs and ΣDienes) between maternal blood and eggs, indicating that these chemicals are transferred from nesting females to eggs and, ultimately, to hatchlings. OCPs may, therefore, have an effect on the health and reproductive performance of hawksbill turtles, both in terms of their fertility and egg development. Conservation strategies need to be species-specific, due to differences in feeding, and address the reasons for any decline, focusing on regional assessments. Thus, accurate and comparable monitoring data are necessary, which requires the standardization of monitoring protocols.

Microbiota acquired during labor and through the first days of life contributes to the newborn’s immune maturation and development. Mother provides probiotics and prebiotics factors through colostrum and maternal milk to shape the first neonatal microbiota. Previous works have reported that immunoglobulin A (IgA) secreted in colostrum is coating a fraction of maternal microbiota. Thus, to better characterize this IgA-microbiota association, we used flow cytometry coupled with 16S rRNA gene sequencing (IgA-Seq) in human colostrum and neonatal feces. We identified IgA bound bacteria (IgA+) and characterized their diversity and composition shared in colostrum fractions and neonatal fecal bacteria. We found that IgA2 is mainly associated with Bifidobacterium, Pseudomonas, Lactobacillus, and Paracoccus, among other genera shared in colostrum and neonatal fecal samples. We found that metabolic pathways related to epithelial adhesion and carbohydrate consumption are enriched within the IgA2+ fecal microbiota. The association of IgA2 with specific bacteria could be explained because these antibodies recognize common antigens expressed on the surface of these bacterial genera. Our data suggest a preferential targeting of commensal bacteria by IgA2, revealing a possible function of maternal IgA2 in the shaping of the fecal microbial composition in the neonate during the first days of life.

Hindgut microorganisms in newborn calves play an important role in the development of immunity and metabolism, and optimization of performance. However, knowledge of the extent to which microbiome colonization of the calf intestine is dependent on maternal characteristics is limited. In this study, placenta, umbilical cord, amniotic fluid, colostrum, cow feces, and calf meconium samples were collected from 6 Holstein cow-calf pairs. Microbial composition was analyzed by 16S rRNA gene high-throughput sequencing, and maternal transfer characteristics assessed using SourceTracker based on Gibbs sampling to fit the joint distribution using the mean proportions of each sample with meconium as the “sink” and other sample types as different “sources.” Alpha and beta diversity analyses revealed sample type-specific microbiome features: microbial composition of the placenta, umbilical cord, amniotic fluid, colostrum, and calf feces were similar, but differed from cow feces (p < 0.05). Compared with profiles of meconium vs. placenta, meconium vs. umbilical cord, and meconium vs. colostrum, differences between the meconium and amniotic fluid were most obvious. SourceTracker analysis revealed that 23.8 ± 2.21% of the meconium OTUs matched those of umbilical cord samples, followed by the meconium-placenta pair (15.57 ± 2.2%), meconium-colostrum pair (14.4 ± 1.9%), and meconium-amniotic fluid pair (11.2 ± 1.7%). The matching ratio between meconium and cow feces was the smallest (10.5 ± 1%). Overall, our data indicated that the composition of the meconium microflora was similar compared with multiple maternal sites including umbilical cord, placenta, colostrum, and amniotic fluid. The umbilical cord microflora seemed to contribute the most to colonization of the fecal microflora of calves. Bacteria with digestive functions such as cellulose decomposition and rumen fermentation were mainly transmitted during the maternal transfer process.

Microbiota acquired during labor and through the first days of life contributes to the newborn's immune maturation and development. Mother provides probiotics and prebiotics factors through colostrum and maternal milk to shape the first neonatal microbiota. Previous works have reported that immunoglobulin A (IgA) secreted in colostrum is coating a fraction of maternal microbiota. Thus, to better characterize this IgA-microbiota association, we used flow cytometry coupled with 16S rRNA gene sequencing (IgA-Seq) in human colostrum and neonatal feces. We identified IgA bound bacteria (IgA+) and characterized their diversity and composition shared in colostrum fractions and neonatal fecal bacteria. We found that IgA2 is mainly associated with Bifidobacterium , Pseudomonas, Lactobacillus , and Paracoccus , among other genera shared in colostrum and neonatal fecal samples. We found that metabolic pathways related to epithelial adhesion and carbohydrate consumption are enriched within the IgA2+ fecal microbiota. The association of IgA2 with specific bacteria could be explained because these antibodies recognize common antigens expressed on the surface of these bacterial genera. Our data suggest a preferential targeting of commensal bacteria by IgA2, revealing a possible function of maternal IgA2 in the shaping of the fecal microbial composition in the neonate during the first days of life. [ABSTRACT FROM AUTHOR]

Abstract Background Cryptosporidium parvum is a major cause of diarrhea in children and ruminants at the earliest stages of life. Maternal antibodies represent the main shield of neonate mammals for most of the infections. Two recombinant antigens (SA35 and SA40), portions of two C. parvum proteins, were tested for their ability to induce immune responses in adult mice and for protection on neonate BALB/c mice born from females immunised by mucosal delivery of both peptides. Methods Adult BALB/c mice were intraperitoneally immunised with SA35 and SA40, separately or mixed, and their immune response was characterised. Furthermore, BALB/c pregnant mice were immunised by mucosal delivery with an SA35/40 mix, before and during pregnancy. Soon after birth, their offspring were infected with two doses (1 × 105 and 5 × 103) of C. parvum oocysts and the parasitic burden was determined at 5 and 9 days post-infection. Results Intraperitoneal immunisation with SA35 and SA40 induced specific IgG and IgG1 in serum, specific IgA in the intestinal mucosa, increase of CD3+/CD4+ and CD30+ cells in splenocytes, which produced IFN-γ. Neonates born from immunised mice and infected with 1 × 105 oocysts showed a significant reduction of oocysts and intestinal forms (23 and 42%, respectively). A reduction of all parasitic forms (96%; P

Urban herring gulls (Larus argentatus) are exposed to contaminants from aquatic, terrestrial and anthropogenic sources. We aim to assess if differences in urbanisation affect ecological niche and contaminant concentrations in female herring gulls. Furthermore, we investigated maternal transfer from mothers to eggs for all the target compounds, including chlorinated paraffins (CPs) and cyclic volatile methyl siloxane (cVMSs), which to our knowledge have not been assessed in herring gulls previously. We compare concentrations of legacy and emerging contaminants and metals in blood and eggs between two herring gull colonies located 51 km apart, in the urban influenced Norwegian Oslofjord. While both colonies are within an urbanised area, the inner fjord is more so, as it is surrounded by Oslo, the capital and largest city in Norway Stable isotopes of carbon and nitrogen indicated a more marine ecological niche in the outer than the inner fjord colony, although with overlap. Persistent organic pollutant (POP) concentrations were similar in the inner and outer fjord colonies, while the short-chained chlorinated paraffins (SCCP), which are recently added to the Stockholm convention and contaminants of emerging concern (CECs) varied, with higher concentrations of SCCP and the cVMS decamethylcyclopentasiloxane (D5) in females and eggs of the inner fjord colony. Per- and polyfluorinated substances (PFAS) concentrations were higher in the outer fjord colony, likely linked to releases from a point-source (airport and waste management facility with open access to food waste). In blood, chlorinated paraffins contributed most the total lipophilic contaminants (inner: 78%, outer: 56%), while polychlorinated biphenyls (PCBs) were the most abundant lipophilic contaminants in eggs (inner: 62%, outer: 46%). Dechloranes and brominated flame retardants (BFRs) were detected in few samples. Maternal transfer, assessed by egg to blood ratios, of cVMSs were similar to the POPs with mean log ratio 0.39 (D5), while it was lower for SCCPs, with log ratios-0.77. Our results indicate comparable POP exposure of the herring gulls in the inner and outer Oslofjord, likely due to overlap in ecological niches between the colonies and wide distribution of POPs. The differences between the colonies in concentrations of PFAS, cVMS and CPs shows that point source exposures and urban influence may be more important than ecological niche for these compounds.

Background: The breakdown of dead organic matter is driven by a diverse array of organisms and is an important process increasingly impacted by a range of contaminants. While many studies have documented how contaminants affect food webs that are fueled by decaying plant litters, much less is known about how contaminants affect organisms that rely on dead animal material. Here, we begin to explore the effects of food contamination—using silver nanoparticles (AgNPs) as a model contaminant—on the carrion beetle Nicrophorus vespilloides that buries carcasses of small vertebrates in soils as food source and larval nursing grounds. Results: Our data show that a single ingestion of a non-lethal dose of 1 μg mL−1 AgNPs by adult female beetles does not affect overall gut microbial activity but results in shifts in the gut microbial community composition towards pathogens including Alcaligenes, Morganella, and Pseudomonas. While no effects were observed in offspring clutch size, some reductions were visible in clutch weight, number of larvae, and number of eclosing pupae in exposed N. vespilloides in comparison with controls. Repeated ingestion of AgNPs over several weeks led to a decrease in survival of adult beetles, suggesting that more environmentally realistic exposure scenarios can directly affect the success of carcass-feeding animals. Conclusions: Sub-lethal carcass contamination with a model pollutant can affect the gut microbial composition in female beetles and reduce offspring fitness. This encourages consideration of currently overlooked propagation routes of contaminants through necrophagous food webs and inherent consequences for ecological and evolutionary processes. [ABSTRACT FROM AUTHOR]

Little is known of the movement or presence of unregulated, emerging contaminants in top predators. The aim of the present study was to conduct the first screening of legacy and emerging contaminants in multiple tissues of killer whales (Orcinus orca) from Norway and investigate tissue partitioning and maternal transfer. Blubber was collected from 8 killer whales in 2015 to 2017, in addition to muscle from 5 of the individuals, and kidney, liver, heart, and spleen from a neonate. We screened for 4 unregulated brominated flame retardants and found pentabromotoluene (PBT) and hexabromobenzene (HBB) at low levels in the blubber of all individuals (median PBT 0.091 ng/g lipid wt, median HBB 1.4 ng/g lipid wt). Levels of PBT and HBB (wet wt) were twice as high in the blubber than the muscle for each individual, confirming preferential accumulation in lipid‐rich tissues. Perfluoroalkyl substances and total mercury levels were lower in the neonate than adults, suggesting less efficient maternal transfer of these substances. Polychlorinated biphenyl levels in blubber exceeded the threshold for onset of physiological effects (9 µg/g lipid wt) in 7 of the 8 whales, including the neonate. The presence of PBT and HBB in the neonate is the first evidence of maternal transfer of these unregulated contaminants in marine mammals. Our results are relevant for the continued environmental monitoring of contaminants in the Arctic. Environ Toxicol Chem 2021;40:1850–1860. © 2021 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. [ABSTRACT FROM AUTHOR]

In oviparous species, pesticides may affect embryo survival via maternal transfer or contaminant absorption from the soil, thus representing an important cause of population decline. Maternal transfer is a source of contamination during vitellogenesis and oviductal egg retention. Currently, there is still limited evidence of the potential risk of embryonic exposure routes in reptiles. We investigated whether different agricultural treatments affect embryo, egg, and hatchling development and survival in the Italian wall lizard. We kept gravid females from conventional and control fields in captivity until oviposition and, after eggs being incubated in a pesticide-free environment, offspring morphology and performance (running speed) were assessed. Our results showed that the size of the mother positively influences the number of the eggs and offspring, as well as hatching body condition. Bigger females from conventional treatments tended to lay more eggs. Moreover, at equal female body conditions, eggs and hatchlings from conventional treatments had worse quality (i.e., smaller size and lower body condition) than those from control areas. No effect of treatment was observed on hatchling locomotor performance. In conclusion, our study provided new insights of the direct and indirect effects of field management (i.e., pesticide exposure) on females' reproductive success through the alteration of female's behaviour, which in turn may affect offspring development and health. [ABSTRACT FROM AUTHOR]

Early-life microbiota has been linked to the development of chronic inflammatory diseases. It has been hypothesized that maternal vaginal microbiota is an important initial seeding source and therefore might have lifelong effects on disease risk. To understand maternal vaginal microbiota’s role in seeding the child’s microbiota and the extent of delivery mode-dependent transmission, we studied 665 mother–child dyads from the COPSAC2010 cohort. The maternal vaginal microbiota was evaluated twice in the third trimester and compared with the children’s fecal (at 1 week, 1 month, and 1 year of age) and airway microbiota (at 1 week, 1 month, and 3 months). Based on the concept of weighted transfer ratios (WTRs), we have identified bacterial orders for which the WTR displays patterns indicate persistent or transient transfer from the maternal vaginal microbiome, as well as orders that are shared at later time points independent of delivery mode, indicating a common reservoir.

As a new group of persistent organic pollutants of concern, chlorinated paraffins (CPs) have been widely detected in the environment and biota, but their occurrence, partitioning, and transfer in humans have been not well documented. In this study, 32 pairs of maternal blood, cord blood, and placenta samples were collected from pregnant women in South China, and the blood was further separated into plasma and red blood cells (RBCs) for blood partitioning study. Short- and medium-chain CPs (SCCPs and MCCPs, respectively) were detected in all the five human biological matrices, suggesting prevalent exposure and maternal transfer of CPs in the pregnant women. Discrepant congener group profiles of CPs were observed in different human biological matrices. Significant differences in the plasma-RBC partitioning of CPs in the maternal and cord bloods were identified (p

There is growing recognition of the ways in which maternal effects can influence offspring size, physiological performance, and survival. Additionally, environmental contaminants increasingly act as stressors in maternal environments, possibly leading to maternal effects on subsequent offspring. Thus, it is important to determine whether contaminants and other stressors can contribute to maternal effects, particularly under varied ecological conditions that encompass the range under which offspring develop. We used aquatic mesocosms to determine whether maternal effects of mercury (Hg) exposure shape offspring phenotype in the American toad (Bufo americanus) in the presence or absence of larval predators (dragonfly naiads). We found significant maternal effects of Hg exposure and significant effects of predators on several offspring traits, but there was little evidence that maternal effects altered offspring interactions with predators. Offspring from Hg-exposed mothers were 18% smaller than those of reference mothers. Offspring reared with predators were 23% smaller at metamorphosis than those reared without predators. There was also evidence of reduced larval survival when larvae were reared with predators, but this was independent of maternal effects. Additionally, 5 times more larvae had spinal malformations when reared without predators, suggesting selective predation of malformed larvae by predators. Lastly, we found a significant negative correlation between offspring survival and algal density in mesocosms, indicating a role for top-down effects of predators on periphyton communities. Our results demonstrate that maternal exposure to an environmental stressor can induce phenotypic responses in offspring in a direction similar to that produced by direct exposure of offspring to predators.

Turtles are a potential sentinel species of aquatic ecosystem health as they inhabit aquatic ecosystems, are long lived, and potentially have high exposure to anthropogenic chemicals via food and water. This study investigated per- and polyfluoroalkyl substances (PFAS) tissue partitioning in female Emydura macquarii macquarii turtle, and the maternal offloading of (PFAS) into eggs and then hatchlings as well as the accumulation of PFAS in male and female Emydura macquarii macquarii serum. Significantly higher levels of perfluorosulfonic acids (PFSAs) and perfluorocarboxylic acids (PFCAs) were measured in the male serum compared to the female turtle serum, whereas perfluoroalkane sulfonamides (FASAs) were significantly higher in the female turtle serum. Perfluorooctane sulfonate (PFOS) was the predominant PFAS in the turtles whereas PFHxA was the predominant PFAS found in the surrounding water. PFHxA was not reported in any turtle tissue or the serum. The short-chain PFSAs and FASAs appeared to be highly associated with blood; long-chain PFSAs and PFCAs were more likely to be associated with tissue. Half of the PFHxS and all the long-chain PFSAs and PFCAs reported in the yolks were transferred into the hatchlings (by mass), suggesting a potential intergenerational effect. [Display omitted] • Half the PFHxS and all long-chain PFSAs and PFCAs were transferred from yolk to hatchlings (by mass). • PFOS was the dominant PFAS in the turtle body, making up approximately 90% of the total PFAS. • PFOS was found at the highest concentration in the ovaries and the lowest in the muscle. • PFSAs and PFCAs were found at higher concentration in the serum of males, FASAs in the females. • Elevated PFHxA, PFPeA and PFHpA were found in the water but were not in any serum or tissue samples. [ABSTRACT FROM AUTHOR]

Over the last two decades, the discovery of antibodies directed against neuronal surface antigens (NSA-Abs) in patients with different forms of encephalitis has provided a basis for immunotherapies in previously undefined disorders. Nevertheless, despite the circumstantial clinical evidence of the pathogenic role of these antibodies in classical autoimmune encephalitis, specific criteria need to be applied in order to establish the autoimmune nature of a disease. A growing number of studies have begun to provide proof of the pathogenicity of NSA-Abs and insights into their pathogenic mechanisms through passive transfer or, more rarely, through active immunization animal models. Moreover, the increasing evidence that NSA-Abs in the maternal circulation can reach the fetal brain parenchyma during gestation, causing long-term effects, has led to models of antibody-induced neurodevelopmental disorders. This review summarizes different methodological approaches and the results of the animal models of N-methyl-d-aspartate receptor (NMDAR), leucine-rich glioma-inactivated 1 (LGI1), contactin-associated protein 2 (CASPR2), and α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptor (AMPAR) antibody-mediated disorders and discuss the results and the limitations. We also summarize recent experiments that demonstrate that maternal antibodies to NMDAR and CASPR2 can alter development in the offspring with potential lifelong susceptibility to neurological or psychiatric disorders.

Despite the increasing health risks shown by the continuous detection of organophosphate esters (OPEs) in biota in recent years, information on the occurrence and potential risks of OPEs in marine mammals remains limited. This study conducted the first investigation into the body burdens and potential risks of 10 traditional OPEs (tOPEs) and five emerging OPEs (eOPEs) in 10 cetacean species ( n = 84) from the northern South China Sea (NSCS) during 2005-2021. All OPEs, except for 2-ethylhexyl diphenyl phosphate (EHDPHP), were detected in these cetaceans, indicating their widespread occurrence in the NSCS. Although the levels of the ∑ 10 tOPEs in humpback dolphins remained stable from 2005 to 2021, the concentrations of the ∑ 5 eOPEs showed a significant increase, suggesting a growing demand for these new-generation OPEs in South China. Dolphins in proximity to urban regions generally exhibited higher OPE concentrations than those from rural areas, mirroring the environmental trends of OPEs occurring in this area. All OPE congeners, except for EHDPHP, in humpback dolphins exhibited a maternal transfer ratio >1, indicating that the dolphin placenta may not be an efficient barrier for OPEs. The observed significant correlations between levels of OPEs and hormones (triiodothyronine, thyroxine, and testosterone) in humpback dolphins indicated that OPE exposures might have endocrine disruption effects on the dolphin population.

In the present research, two studies were performed to determine the effects of specific botanical compounds (BCs) on the innate feed preference and feed intake of piglets, as follows: Exp. 1 studied the innate feed preferences of post-weaning piglets using a double-choice feeding test. A total of 828 weaned piglets were distributed into 36 pens (23 pigs/pen) and assigned to three dietary pair choice feeding options (n = 12): unsupplemented prestarter diets (reference) versus reference plus D-limonene, trans-anethole, or eucalyptol. Piglets showed a preference for diets with D-limonene (53.8%) and trans-anethole (54.5%), and an aversion to eucalyptol (41.6%) (p < 0.05). Exp. 2 studied whether the prenatal and perinatal exposure to D-limonene, trans-anethole, and eucalyptol influences the feed intake and growth of newly-weaned piglets. Twenty-eight gestating and lactating sows were distributed into two dietary treatments (n = 14): unsupplemented Control diets or Control plus a blend of BCs (BBC; containing D-limonene, trans-anethole, and eucalyptol). D-limonene, trans-anethole, and eucalyptol were transferred into the placental fluid, and D-limonene and trans-anethole into the milk (p < 0.05). Furthermore, weanling piglets (n = 200; Control) and (n = 203; BBC) received the same treatment as their mothers in prestarter diets. The early response after weaning showed that piglets’ post-weaning BW gain was higher in the Control (p < 0.05) group than in those exposed to BBC. In conclusion, prenatal exposure to preferred D-limonene and trans-anethole, or familiarity to eucalyptol did not help to overcome the innate aversion to eucalyptol and its negative effect on weanling piglets’ BW.